Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28454.t1 | XP_003630160.2 | 99.5 | 438 | 2 | 0 | 1 | 438 | 4 | 441 | 2.60E-239 | 837.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28454.t1 | Q9FIB6 | 82.1 | 435 | 77 | 1 | 4 | 437 | 7 | 441 | 5.4e-201 | 701.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28454.t1 | B7FLC5 | 99.5 | 438 | 2 | 0 | 1 | 438 | 4 | 441 | 1.9e-239 | 837.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene28454 | 0.874272 | 7.69E-68 | -1.69E-46 |
| MS.gene04918 | MS.gene28454 | 0.81799 | 2.48E-52 | -1.69E-46 |
| MS.gene049193 | MS.gene28454 | 0.802972 | 4.35E-49 | -1.69E-46 |
| MS.gene04920 | MS.gene28454 | 0.810962 | 8.88E-51 | -1.69E-46 |
| MS.gene049254 | MS.gene28454 | 0.822754 | 2.00E-53 | -1.69E-46 |
| MS.gene049283 | MS.gene28454 | 0.836072 | 1.16E-56 | -1.69E-46 |
| MS.gene049343 | MS.gene28454 | 0.803743 | 3.01E-49 | -1.69E-46 |
| MS.gene049350 | MS.gene28454 | 0.807844 | 4.15E-50 | -1.69E-46 |
| MS.gene049368 | MS.gene28454 | 0.81887 | 1.56E-52 | -1.69E-46 |
| MS.gene049499 | MS.gene28454 | 0.816101 | 6.58E-52 | -1.69E-46 |
| MS.gene049711 | MS.gene28454 | 0.804985 | 1.66E-49 | -1.69E-46 |
| MS.gene049744 | MS.gene28454 | 0.824374 | 8.36E-54 | -1.69E-46 |
| MS.gene049807 | MS.gene28454 | 0.82305 | 1.71E-53 | -1.69E-46 |
| MS.gene049983 | MS.gene28454 | 0.831501 | 1.61E-55 | -1.69E-46 |
| MS.gene050213 | MS.gene28454 | 0.826171 | 3.14E-54 | -1.69E-46 |
| MS.gene050257 | MS.gene28454 | 0.80321 | 3.89E-49 | -1.69E-46 |
| MS.gene050258 | MS.gene28454 | 0.822946 | 1.80E-53 | -1.69E-46 |
| MS.gene050414 | MS.gene28454 | 0.801906 | 7.22E-49 | -1.69E-46 |
| MS.gene050551 | MS.gene28454 | 0.809595 | 1.75E-50 | -1.69E-46 |
| MS.gene050556 | MS.gene28454 | 0.803292 | 3.74E-49 | -1.69E-46 |
| MS.gene050646 | MS.gene28454 | 0.819491 | 1.13E-52 | -1.69E-46 |
| MS.gene050654 | MS.gene28454 | 0.826147 | 3.18E-54 | -1.69E-46 |
| MS.gene050901 | MS.gene28454 | 0.803256 | 3.80E-49 | -1.69E-46 |
| MS.gene050945 | MS.gene28454 | 0.800938 | 1.14E-48 | -1.69E-46 |
| MS.gene051003 | MS.gene28454 | 0.804511 | 2.09E-49 | -1.69E-46 |
| MS.gene051198 | MS.gene28454 | 0.806245 | 9.03E-50 | -1.69E-46 |
| MS.gene051234 | MS.gene28454 | 0.812573 | 3.96E-51 | -1.69E-46 |
| MS.gene051343 | MS.gene28454 | 0.855608 | 5.63E-62 | -1.69E-46 |
| MS.gene051744 | MS.gene28454 | 0.860714 | 1.70E-63 | -1.69E-46 |
| MS.gene051975 | MS.gene28454 | 0.839932 | 1.18E-57 | -1.69E-46 |
| MS.gene052016 | MS.gene28454 | 0.80417 | 2.46E-49 | -1.69E-46 |
| MS.gene052037 | MS.gene28454 | 0.823255 | 1.53E-53 | -1.69E-46 |
| MS.gene052118 | MS.gene28454 | 0.81273 | 3.66E-51 | -1.69E-46 |
| MS.gene052307 | MS.gene28454 | 0.854502 | 1.18E-61 | -1.69E-46 |
| MS.gene052463 | MS.gene28454 | 0.839159 | 1.87E-57 | -1.69E-46 |
| MS.gene052609 | MS.gene28454 | 0.888572 | 5.24E-73 | -1.69E-46 |
| MS.gene052717 | MS.gene28454 | 0.803882 | 2.82E-49 | -1.69E-46 |
| MS.gene052775 | MS.gene28454 | 0.839915 | 1.19E-57 | -1.69E-46 |
| MS.gene052776 | MS.gene28454 | 0.83699 | 6.77E-57 | -1.69E-46 |
| MS.gene052855 | MS.gene28454 | 0.829405 | 5.24E-55 | -1.69E-46 |
| MS.gene052932 | MS.gene28454 | 0.800999 | 1.11E-48 | -1.69E-46 |
| MS.gene053079 | MS.gene28454 | 0.839242 | 1.78E-57 | -1.69E-46 |
| MS.gene053143 | MS.gene28454 | 0.858296 | 9.08E-63 | -1.69E-46 |
| MS.gene053226 | MS.gene28454 | 0.818744 | 1.67E-52 | -1.69E-46 |
| MS.gene053620 | MS.gene28454 | 0.825102 | 5.63E-54 | -1.69E-46 |
| MS.gene054142 | MS.gene28454 | 0.807051 | 6.10E-50 | -1.69E-46 |
| MS.gene054178 | MS.gene28454 | 0.805603 | 1.23E-49 | -1.69E-46 |
| MS.gene054576 | MS.gene28454 | 0.820511 | 6.60E-53 | -1.69E-46 |
| MS.gene05458 | MS.gene28454 | 0.803419 | 3.52E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene28454 | 0.806873 | 6.66E-50 | -1.69E-46 |
| MS.gene054709 | MS.gene28454 | 0.817895 | 2.60E-52 | -1.69E-46 |
| MS.gene054824 | MS.gene28454 | 0.807605 | 4.66E-50 | -1.69E-46 |
| MS.gene054892 | MS.gene28454 | 0.812969 | 3.24E-51 | -1.69E-46 |
| MS.gene054893 | MS.gene28454 | 0.809491 | 1.84E-50 | -1.69E-46 |
| MS.gene054943 | MS.gene28454 | 0.801444 | 8.98E-49 | -1.69E-46 |
| MS.gene055231 | MS.gene28454 | 0.805713 | 1.17E-49 | -1.69E-46 |
| MS.gene055313 | MS.gene28454 | 0.81441 | 1.56E-51 | -1.69E-46 |
| MS.gene055513 | MS.gene28454 | 0.912784 | 1.31E-83 | -1.69E-46 |
| MS.gene055864 | MS.gene28454 | 0.807628 | 4.61E-50 | -1.69E-46 |
| MS.gene056045 | MS.gene28454 | 0.824668 | 7.12E-54 | -1.69E-46 |
| MS.gene056050 | MS.gene28454 | 0.81874 | 1.67E-52 | -1.69E-46 |
| MS.gene056174 | MS.gene28454 | 0.824272 | 8.83E-54 | -1.69E-46 |
| MS.gene056181 | MS.gene28454 | 0.826872 | 2.14E-54 | -1.69E-46 |
| MS.gene056308 | MS.gene28454 | 0.867098 | 1.76E-65 | -1.69E-46 |
| MS.gene056507 | MS.gene28454 | 0.843046 | 1.78E-58 | -1.69E-46 |
| MS.gene056519 | MS.gene28454 | 0.824598 | 7.40E-54 | -1.69E-46 |
| MS.gene056699 | MS.gene28454 | 0.882687 | 8.45E-71 | -1.69E-46 |
| MS.gene056787 | MS.gene28454 | 0.807298 | 5.41E-50 | -1.69E-46 |
| MS.gene056831 | MS.gene28454 | 0.826618 | 2.46E-54 | -1.69E-46 |
| MS.gene05686 | MS.gene28454 | 0.820653 | 6.12E-53 | -1.69E-46 |
| MS.gene056973 | MS.gene28454 | 0.83301 | 6.82E-56 | -1.69E-46 |
| MS.gene056986 | MS.gene28454 | 0.80439 | 2.21E-49 | -1.69E-46 |
| MS.gene057034 | MS.gene28454 | 0.876709 | 1.13E-68 | -1.69E-46 |
| MS.gene057102 | MS.gene28454 | 0.846457 | 2.15E-59 | -1.69E-46 |
| MS.gene057208 | MS.gene28454 | 0.813495 | 2.49E-51 | -1.69E-46 |
| MS.gene057329 | MS.gene28454 | 0.805438 | 1.33E-49 | -1.69E-46 |
| MS.gene057855 | MS.gene28454 | 0.801714 | 7.91E-49 | -1.69E-46 |
| MS.gene057893 | MS.gene28454 | -0.802631 | 5.12E-49 | -1.69E-46 |
| MS.gene057919 | MS.gene28454 | 0.803532 | 3.33E-49 | -1.69E-46 |
| MS.gene058010 | MS.gene28454 | 0.821225 | 4.52E-53 | -1.69E-46 |
| MS.gene058289 | MS.gene28454 | 0.840438 | 8.69E-58 | -1.69E-46 |
| MS.gene058292 | MS.gene28454 | 0.814005 | 1.92E-51 | -1.69E-46 |
| MS.gene058330 | MS.gene28454 | 0.81699 | 4.16E-52 | -1.69E-46 |
| MS.gene05847 | MS.gene28454 | 0.813359 | 2.66E-51 | -1.69E-46 |
| MS.gene058649 | MS.gene28454 | 0.810983 | 8.79E-51 | -1.69E-46 |
| MS.gene058781 | MS.gene28454 | 0.809511 | 1.83E-50 | -1.69E-46 |
| MS.gene059146 | MS.gene28454 | 0.810408 | 1.17E-50 | -1.69E-46 |
| MS.gene059170 | MS.gene28454 | 0.816653 | 4.95E-52 | -1.69E-46 |
| MS.gene059439 | MS.gene28454 | 0.834399 | 3.07E-56 | -1.69E-46 |
| MS.gene059570 | MS.gene28454 | 0.809933 | 1.48E-50 | -1.69E-46 |
| MS.gene059778 | MS.gene28454 | 0.84367 | 1.21E-58 | -1.69E-46 |
| MS.gene05985 | MS.gene28454 | -0.852389 | 4.77E-61 | -1.69E-46 |
| MS.gene059965 | MS.gene28454 | 0.832709 | 8.10E-56 | -1.69E-46 |
| MS.gene060435 | MS.gene28454 | 0.800544 | 1.37E-48 | -1.69E-46 |
| MS.gene060522 | MS.gene28454 | 0.816976 | 4.19E-52 | -1.69E-46 |
| MS.gene060527 | MS.gene28454 | 0.810139 | 1.34E-50 | -1.69E-46 |
| MS.gene060777 | MS.gene28454 | 0.829294 | 5.58E-55 | -1.69E-46 |
| MS.gene060932 | MS.gene28454 | 0.80829 | 3.33E-50 | -1.69E-46 |
| MS.gene060933 | MS.gene28454 | 0.852054 | 5.94E-61 | -1.69E-46 |
| MS.gene060935 | MS.gene28454 | 0.842206 | 2.98E-58 | -1.69E-46 |
| MS.gene060937 | MS.gene28454 | 0.857605 | 1.46E-62 | -1.69E-46 |
| MS.gene061006 | MS.gene28454 | 0.810071 | 1.38E-50 | -1.69E-46 |
| MS.gene061100 | MS.gene28454 | 0.872436 | 3.19E-67 | -1.69E-46 |
| MS.gene061160 | MS.gene28454 | 0.801228 | 9.94E-49 | -1.69E-46 |
| MS.gene061188 | MS.gene28454 | 0.820214 | 7.72E-53 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28454.t1 | MTR_8g092450 | 99.543 | 438 | 2 | 0 | 1 | 438 | 4 | 441 | 0.0 | 887 |
| MS.gene28454.t1 | MTR_8g092390 | 97.489 | 438 | 11 | 0 | 1 | 438 | 1 | 438 | 0.0 | 870 |
| MS.gene28454.t1 | MTR_5g072475 | 48.352 | 91 | 47 | 0 | 98 | 188 | 35 | 125 | 1.16e-14 | 72.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28454.t1 | AT5G09900 | 82.069 | 435 | 77 | 1 | 4 | 437 | 7 | 441 | 0.0 | 724 |
| MS.gene28454.t1 | AT5G09900 | 82.069 | 435 | 77 | 1 | 4 | 437 | 7 | 441 | 0.0 | 724 |
| MS.gene28454.t1 | AT5G64760 | 79.310 | 435 | 89 | 1 | 4 | 437 | 7 | 441 | 0.0 | 694 |
| MS.gene28454.t1 | AT5G64760 | 79.310 | 435 | 89 | 1 | 4 | 437 | 7 | 441 | 0.0 | 694 |
| MS.gene28454.t1 | AT5G64760 | 80.048 | 421 | 83 | 1 | 18 | 437 | 1 | 421 | 0.0 | 679 |
| MS.gene28454.t1 | AT5G09900 | 80.178 | 338 | 66 | 1 | 4 | 340 | 7 | 344 | 0.0 | 545 |
Find 93 sgRNAs with CRISPR-Local
Find 276 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACCTTCAAAGCTGCTTTA+TGG | 0.134030 | 4.4:-8454241 | None:intergenic |
| AAACAACGATCTGATCATTT+AGG | 0.167639 | 4.4:-8449838 | None:intergenic |
| GACCATATTATCACCTTCTT+TGG | 0.222140 | 4.4:-8451276 | None:intergenic |
| TGAAATTTGCCGCTGCTATA+AGG | 0.251024 | 4.4:+8453116 | MS.gene28454:CDS |
| CATTGCTATCCTTTGCTGTT+TGG | 0.295822 | 4.4:-8454130 | None:intergenic |
| AACAACGATCTGATCATTTA+GGG | 0.304111 | 4.4:-8449837 | None:intergenic |
| CAAGAAACTTGCCAAGATTA+AGG | 0.314974 | 4.4:+8450821 | MS.gene28454:CDS |
| TCTTATCAGGTGGAAACTTT+TGG | 0.318210 | 4.4:+8450999 | MS.gene28454:intron |
| TCATCCAATGGACAGCTCTT+TGG | 0.322854 | 4.4:+8453549 | MS.gene28454:CDS |
| TGCCTCCTCAATATGTGATA+TGG | 0.330493 | 4.4:+8454441 | MS.gene28454:CDS |
| CATCCAATGGACAGCTCTTT+GGG | 0.333432 | 4.4:+8453550 | MS.gene28454:CDS |
| CCCCTGTTCTTCCTTAATCT+TGG | 0.345178 | 4.4:-8450832 | None:intergenic |
| ACCATATTATCACCTTCTTT+GGG | 0.346638 | 4.4:-8451275 | None:intergenic |
| AGGATAACATACTGGAAGTT+TGG | 0.347252 | 4.4:-8453407 | None:intergenic |
| TATGGACTGAAGTTGCCTTA+TGG | 0.366194 | 4.4:+8454459 | MS.gene28454:CDS |
| GTTCAGGCCATTATGCGTTC+AGG | 0.367944 | 4.4:+8454559 | MS.gene28454:CDS |
| ATGGACTGAAGTTGCCTTAT+GGG | 0.381336 | 4.4:+8454460 | MS.gene28454:CDS |
| TAACTCATGGGCAGCAAACT+TGG | 0.387347 | 4.4:+8454160 | MS.gene28454:CDS |
| TGCCAAGATTAAGGAAGAAC+AGG | 0.388343 | 4.4:+8450830 | MS.gene28454:CDS |
| TTTATTACGAGCTAATGATT+AGG | 0.390801 | 4.4:+8451345 | MS.gene28454:CDS |
| ATTCGACATAGATGCATCTA+AGG | 0.393010 | 4.4:+8451241 | MS.gene28454:CDS |
| TGCAGATATTCCGTCTCTAC+TGG | 0.398939 | 4.4:+8451310 | MS.gene28454:CDS |
| TATCTACCCTGAACGCATAA+TGG | 0.404083 | 4.4:-8454566 | None:intergenic |
| GCCCAAAGAAGGTGATAATA+TGG | 0.427248 | 4.4:+8451274 | MS.gene28454:CDS |
| TTCAAGATAATCATTGTTGT+GGG | 0.433017 | 4.4:-8453097 | None:intergenic |
| GGTCCATAAAGCAGCTTTGA+AGG | 0.436788 | 4.4:+8454238 | MS.gene28454:CDS |
| TGAGAAGCATCTGTCTGATA+TGG | 0.444884 | 4.4:+8454052 | MS.gene28454:CDS |
| ATAATGGCCTGAACGCATAA+TGG | 0.449078 | 4.4:-8454550 | None:intergenic |
| TCAATACGAGTTTCAATATC+CGG | 0.449985 | 4.4:-8450617 | None:intergenic |
| ATGTTGAGATTGAGAGAGCT+AGG | 0.451064 | 4.4:+8450793 | MS.gene28454:CDS |
| GCATCTATGTCGAATACTCT+TGG | 0.459620 | 4.4:-8451233 | None:intergenic |
| TTTCAAGATAATCATTGTTG+TGG | 0.472189 | 4.4:-8453098 | None:intergenic |
| TATGGTTGTTACCAAGGCTC+TGG | 0.489637 | 4.4:+8454070 | MS.gene28454:CDS |
| TCGATTGTGCCTAGACCGCC+AGG | 0.490883 | 4.4:+8451172 | MS.gene28454:CDS |
| GGAGAAACTGCTTGATCTTG+TGG | 0.501917 | 4.4:+8454181 | MS.gene28454:CDS |
| CAAGGTGGAATTTAAAAGAC+TGG | 0.502902 | 4.4:-8453360 | None:intergenic |
| TTTGACAATGAGATGGCCTC+TGG | 0.504799 | 4.4:+8453590 | MS.gene28454:CDS |
| AATCTTTGCAACCAGAGCCT+TGG | 0.507062 | 4.4:-8454081 | None:intergenic |
| GCAAAGATTGACAGACCAAT+GGG | 0.508334 | 4.4:+8454095 | MS.gene28454:CDS |
| TTCAAATGAGCATCCCCATA+AGG | 0.512098 | 4.4:-8454474 | None:intergenic |
| TTGTATCAAAATACTATGCA+AGG | 0.512334 | 4.4:+8453790 | MS.gene28454:CDS |
| GCAATGATGTACTTAACTCA+TGG | 0.517832 | 4.4:+8454147 | MS.gene28454:CDS |
| TTCAGGCCATTATGCGTTCA+GGG | 0.522927 | 4.4:+8454560 | MS.gene28454:CDS |
| ACATTGAACAGTGTATCTGC+TGG | 0.524074 | 4.4:+8450650 | MS.gene28454:CDS |
| GGACATAATCCTGGCGGTCT+AGG | 0.525396 | 4.4:-8451181 | None:intergenic |
| TCTCCCAAAGAGCTGTCCAT+TGG | 0.527502 | 4.4:-8453553 | None:intergenic |
| CAAAGCTGCTTTATGGACCA+TGG | 0.531327 | 4.4:-8454234 | None:intergenic |
| ACGACGCGGTCAGCTCAAGC+AGG | 0.533278 | 4.4:+8449868 | MS.gene28454:CDS |
| TTTGTGCACGGACATAATCC+TGG | 0.535226 | 4.4:-8451190 | None:intergenic |
| GTCTGATATGGTTGTTACCA+AGG | 0.537128 | 4.4:+8454064 | MS.gene28454:CDS |
| TGTAACAGCTATGGTACAGC+AGG | 0.546142 | 4.4:+8450571 | MS.gene28454:CDS |
| TGAAATGTTGCTCACTGTGT+TGG | 0.548481 | 4.4:+8454493 | MS.gene28454:CDS |
| TGGAAACAGACTATTCCCAT+TGG | 0.557174 | 4.4:-8454110 | None:intergenic |
| TGAGATTGAGAGAGCTAGGT+TGG | 0.557330 | 4.4:+8450797 | MS.gene28454:CDS |
| TTCTTGAGAGTATTTGTGCA+CGG | 0.563823 | 4.4:-8451202 | None:intergenic |
| AGTAGAGACGGAATATCTGC+AGG | 0.564085 | 4.4:-8451308 | None:intergenic |
| CATTGAACAGTGTATCTGCT+GGG | 0.568762 | 4.4:+8450651 | MS.gene28454:CDS |
| TGCAAAGATTGACAGACCAA+TGG | 0.572504 | 4.4:+8454094 | MS.gene28454:CDS |
| GAGGAAAATATGTTGGTGCT+TGG | 0.574826 | 4.4:+8453310 | MS.gene28454:CDS |
| GAGAAACAAATGCGTCTCGC+CGG | 0.580321 | 4.4:+8449743 | MS.gene28454:CDS |
| CGTCTCGCCGGCGAAATAGT+CGG | 0.582458 | 4.4:+8449755 | MS.gene28454:CDS |
| ATCACAGGCTGTAACAGCTA+TGG | 0.585065 | 4.4:+8450562 | MS.gene28454:intron |
| CAATGATGTACTTAACTCAT+GGG | 0.587731 | 4.4:+8454148 | MS.gene28454:CDS |
| TCTAGTGCCGACTATTTCGC+CGG | 0.590784 | 4.4:-8449762 | None:intergenic |
| TTTAAAAGACTGGACTGCAT+CGG | 0.598019 | 4.4:-8453350 | None:intergenic |
| TGATTTGATGCAAGAAATTG+CGG | 0.598896 | 4.4:+8450872 | MS.gene28454:CDS |
| AGATCCTGAGGAAAATATGT+TGG | 0.600984 | 4.4:+8453303 | MS.gene28454:intron |
| CCTACAGGAATAAAATCAGT+AGG | 0.603669 | 4.4:-8453171 | None:intergenic |
| AGATAGATTTCTATCCTCCA+AGG | 0.603704 | 4.4:-8453378 | None:intergenic |
| TTTGAAGCAGCTGGTCACCA+TGG | 0.604172 | 4.4:+8453523 | MS.gene28454:CDS |
| TGTTTCAACTCCAGTAGAGA+CGG | 0.610635 | 4.4:-8451320 | None:intergenic |
| CATATATTGCCTTATAGCAG+CGG | 0.613022 | 4.4:-8453125 | None:intergenic |
| GCAATATATTGACGAGACAC+CGG | 0.613740 | 4.4:+8450598 | MS.gene28454:CDS |
| TCAGTCCATATCACATATTG+AGG | 0.615157 | 4.4:-8454446 | None:intergenic |
| AGGTGATAATATGGTCGAAG+AGG | 0.618539 | 4.4:+8451283 | MS.gene28454:CDS |
| GAACGAGTTTGACAATGAGA+TGG | 0.619618 | 4.4:+8453583 | MS.gene28454:CDS |
| GCCAAGATTAAGGAAGAACA+GGG | 0.626902 | 4.4:+8450831 | MS.gene28454:CDS |
| AATTTCTTGCATCAAATCAG+CGG | 0.629438 | 4.4:-8450868 | None:intergenic |
| TCACCATGGAAGTCATCCAA+TGG | 0.630891 | 4.4:+8453537 | MS.gene28454:CDS |
| GAACAGTGTATCTGCTGGGA+AGG | 0.631990 | 4.4:+8450655 | MS.gene28454:CDS |
| AGTCTGTTTCCAAACAGCAA+AGG | 0.634017 | 4.4:+8454121 | MS.gene28454:CDS |
| CTGGACTGCATCGGTGAATG+TGG | 0.634478 | 4.4:-8453341 | None:intergenic |
| GTCCATATCACATATTGAGG+AGG | 0.639905 | 4.4:-8454443 | None:intergenic |
| TCAAATACACAAGGAAACCA+TGG | 0.640379 | 4.4:+8454217 | MS.gene28454:CDS |
| AGAACAGGGGCTTATAGCTG+AGG | 0.642737 | 4.4:+8450845 | MS.gene28454:CDS |
| TGGACTGAAGTTGCCTTATG+GGG | 0.647179 | 4.4:+8454461 | MS.gene28454:CDS |
| TGTTGCTCACTGTGTTGGTG+AGG | 0.668735 | 4.4:+8454498 | MS.gene28454:CDS |
| TCTTGCATATACAGATCCTG+AGG | 0.670391 | 4.4:+8453291 | MS.gene28454:intron |
| GAGCTGTCATCAAATACACA+AGG | 0.670564 | 4.4:+8454208 | MS.gene28454:CDS |
| TAGATTTCTATCCTCCAAGG+TGG | 0.684299 | 4.4:-8453375 | None:intergenic |
| TGTCCATTGGATGACTTCCA+TGG | 0.689019 | 4.4:-8453540 | None:intergenic |
| CCAAGATTAAGGAAGAACAG+GGG | 0.732046 | 4.4:+8450832 | MS.gene28454:CDS |
| GTGCACGGACATAATCCTGG+CGG | 0.759607 | 4.4:-8451187 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTTATTTATTTGTGTTT+GGG | + | chr4.4:8452800-8452819 | MS.gene28454:intron | 10.0% |
| !! | TAAATTTATTTATTTGTGTT+TGG | + | chr4.4:8452799-8452818 | MS.gene28454:intron | 10.0% |
| !!! | TAGTTTATTTCTTTTTTAAA+GGG | + | chr4.4:8453991-8454010 | MS.gene28454:intron | 10.0% |
| !!! | TTAGTTTATTTCTTTTTTAA+AGG | + | chr4.4:8453990-8454009 | MS.gene28454:intron | 10.0% |
| !!! | TTTGAAAATGATTTAATTTT+TGG | - | chr4.4:8452746-8452765 | None:intergenic | 10.0% |
| !! | AAAAAAGAAATAAACTAAGA+TGG | - | chr4.4:8453988-8454007 | None:intergenic | 15.0% |
| !! | AAAAACAATAAAAATACAGA+AGG | - | chr4.4:8450926-8450945 | None:intergenic | 15.0% |
| !! | AAGTTCATAAAATTACTAAT+AGG | - | chr4.4:8450100-8450119 | None:intergenic | 15.0% |
| !! | AATATTACTTCAGAAATAAA+TGG | - | chr4.4:8454374-8454393 | None:intergenic | 15.0% |
| !! | GTTAATAATTAAAAACTTGT+TGG | - | chr4.4:8451922-8451941 | None:intergenic | 15.0% |
| !!! | AGTTTATTTCTTTTTTAAAG+GGG | + | chr4.4:8453992-8454011 | MS.gene28454:intron | 15.0% |
| !!! | TTCTGTATTTTTATTGTTTT+TGG | + | chr4.4:8450925-8450944 | MS.gene28454:intron | 15.0% |
| !! | AACTTATATCATTAGTAACA+AGG | - | chr4.4:8452006-8452025 | None:intergenic | 20.0% |
| !! | AATAAGTTGTTTATCCAAAA+AGG | - | chr4.4:8450201-8450220 | None:intergenic | 20.0% |
| !! | AATTATACAACAACAGTATA+TGG | - | chr4.4:8450486-8450505 | None:intergenic | 20.0% |
| !! | ATATTTGAACATGATTAGAA+AGG | - | chr4.4:8453449-8453468 | None:intergenic | 20.0% |
| !! | CAAATATAAATAATTGCAGT+CGG | - | chr4.4:8451676-8451695 | None:intergenic | 20.0% |
| !! | TACTATAAGATTCTGTTATA+CGG | + | chr4.4:8453228-8453247 | MS.gene28454:intron | 20.0% |
| !! | TTAATCATAATGCTTATTTC+TGG | + | chr4.4:8454408-8454427 | MS.gene28454:intron | 20.0% |
| !! | TTTCAATTGTAGATTTCTTA+TGG | + | chr4.4:8449992-8450011 | MS.gene28454:intron | 20.0% |
| !!! | AAAACATATGTCCTTTAAAA+TGG | - | chr4.4:8450739-8450758 | None:intergenic | 20.0% |
| !!! | AAGTTCAAACTCATTTTATA+TGG | - | chr4.4:8452387-8452406 | None:intergenic | 20.0% |
| !!! | AGTATGTAATTTTTGAAGAA+TGG | + | chr4.4:8454337-8454356 | MS.gene28454:intron | 20.0% |
| !!! | ATAAAAAAGTGTAAGGAATT+CGG | - | chr4.4:8453679-8453698 | None:intergenic | 20.0% |
| !!! | ATTTTTAAGAACAGTATCAA+TGG | - | chr4.4:8452470-8452489 | None:intergenic | 20.0% |
| !!! | CTTAATTAAGTTTTATCTGT+AGG | + | chr4.4:8450269-8450288 | MS.gene28454:intron | 20.0% |
| !!! | TTCTAATAAGTTACTGATTT+GGG | + | chr4.4:8451123-8451142 | MS.gene28454:intron | 20.0% |
| !!! | TTTCTAATAAGTTACTGATT+TGG | + | chr4.4:8451122-8451141 | MS.gene28454:intron | 20.0% |
| ! | AAGATTTGATACAAAATCGA+TGG | - | chr4.4:8452210-8452229 | None:intergenic | 25.0% |
| ! | ACACAATACAACATACAAAT+TGG | - | chr4.4:8450763-8450782 | None:intergenic | 25.0% |
| ! | AGATTTGATACAAAATCGAT+GGG | - | chr4.4:8452209-8452228 | None:intergenic | 25.0% |
| ! | AGCATAAGGAAAAAAAAATC+AGG | - | chr4.4:8453047-8453066 | None:intergenic | 25.0% |
| ! | AGTAATGTAGAGTAAAGAAT+TGG | + | chr4.4:8452851-8452870 | MS.gene28454:intron | 25.0% |
| ! | CAAAGAAAAAACTATATCCT+TGG | + | chr4.4:8453927-8453946 | MS.gene28454:intron | 25.0% |
| ! | GAGAAGATAATAAAACAGAA+TGG | - | chr4.4:8450704-8450723 | None:intergenic | 25.0% |
| ! | GATTCCAAAATTAACAGTAT+AGG | - | chr4.4:8451619-8451638 | None:intergenic | 25.0% |
| ! | GTATATGCAAGAACAATATT+TGG | - | chr4.4:8453283-8453302 | None:intergenic | 25.0% |
| ! | GTTATCTTGTACTGTATTTA+TGG | + | chr4.4:8452078-8452097 | MS.gene28454:intron | 25.0% |
| ! | TCTTCTACGTTAAATTGTTT+AGG | + | chr4.4:8451148-8451167 | MS.gene28454:intron | 25.0% |
| ! | TGGAATTGGAAAGAAAATAA+GGG | - | chr4.4:8452265-8452284 | None:intergenic | 25.0% |
| ! | TTATGCAAGAGTAATTGATT+AGG | - | chr4.4:8454022-8454041 | None:intergenic | 25.0% |
| ! | TTCAAGATAATCATTGTTGT+GGG | - | chr4.4:8453100-8453119 | None:intergenic | 25.0% |
| ! | TTGGAATTGGAAAGAAAATA+AGG | - | chr4.4:8452266-8452285 | None:intergenic | 25.0% |
| ! | TTGTATCAAAATACTATGCA+AGG | + | chr4.4:8453790-8453809 | MS.gene28454:CDS | 25.0% |
| ! | TTTATTACGAGCTAATGATT+AGG | + | chr4.4:8451345-8451364 | MS.gene28454:CDS | 25.0% |
| ! | TTTCAAGATAATCATTGTTG+TGG | - | chr4.4:8453101-8453120 | None:intergenic | 25.0% |
| ! | TTTCAGAATAGTTAAGAACT+GGG | - | chr4.4:8452341-8452360 | None:intergenic | 25.0% |
| !! | ATCCTTTTTATCTATATCTG+TGG | + | chr4.4:8451812-8451831 | MS.gene28454:intron | 25.0% |
| !! | ATGTCCTATACTGTTAATTT+TGG | + | chr4.4:8451612-8451631 | MS.gene28454:intron | 25.0% |
| !! | CTTAGCATTATCCATTTTAA+AGG | + | chr4.4:8450725-8450744 | MS.gene28454:intron | 25.0% |
| !! | TCCTTTTTATCTATATCTGT+GGG | + | chr4.4:8451813-8451832 | MS.gene28454:intron | 25.0% |
| !!! | AACTTTGGAAATTTTGGAAT+TGG | - | chr4.4:8452279-8452298 | None:intergenic | 25.0% |
| !!! | AACTTTTTCTTATAGTGTGT+GGG | + | chr4.4:8453893-8453912 | MS.gene28454:intron | 25.0% |
| !!! | ATTTTGGAATCTGAAAGATA+AGG | + | chr4.4:8451628-8451647 | MS.gene28454:intron | 25.0% |
| !!! | GTTTTATTTAATCGATTGGT+AGG | + | chr4.4:8449930-8449949 | MS.gene28454:intron | 25.0% |
| !!! | TAACTTTTTCTTATAGTGTG+TGG | + | chr4.4:8453892-8453911 | MS.gene28454:intron | 25.0% |
| !!! | TATTCTGAAACAAAGCATTA+TGG | + | chr4.4:8452351-8452370 | MS.gene28454:intron | 25.0% |
| !!! | TGATTTTTTTTTCCTTATGC+TGG | + | chr4.4:8453046-8453065 | MS.gene28454:intron | 25.0% |
| !!! | TGTTTTAATTCTTAGCTTGA+TGG | + | chr4.4:8451942-8451961 | MS.gene28454:intron | 25.0% |
| !!! | TTACGTTGTTTTAGACTTAA+AGG | + | chr4.4:8450056-8450075 | MS.gene28454:intron | 25.0% |
| !!! | TTGGGTTTTATTTAATCGAT+TGG | + | chr4.4:8449926-8449945 | MS.gene28454:intron | 25.0% |
| AAACAACGATCTGATCATTT+AGG | - | chr4.4:8449841-8449860 | None:intergenic | 30.0% | |
| AACAACGATCTGATCATTTA+GGG | - | chr4.4:8449840-8449859 | None:intergenic | 30.0% | |
| AAGAAATACTACAACGAAAC+AGG | - | chr4.4:8451388-8451407 | None:intergenic | 30.0% | |
| AATTTCTTGCATCAAATCAG+CGG | - | chr4.4:8450871-8450890 | None:intergenic | 30.0% | |
| AATTTGAGTAAACACAAGCA+AGG | - | chr4.4:8451737-8451756 | None:intergenic | 30.0% | |
| ACAGTGAGAAAATTTGAGAA+AGG | - | chr4.4:8454292-8454311 | None:intergenic | 30.0% | |
| ACCATATTATCACCTTCTTT+GGG | - | chr4.4:8451278-8451297 | None:intergenic | 30.0% | |
| AGCAGCATAAATATAAACGA+CGG | - | chr4.4:8452584-8452603 | None:intergenic | 30.0% | |
| AGTAAGAAATATCATGAACC+AGG | - | chr4.4:8453018-8453037 | None:intergenic | 30.0% | |
| AGTATCAATGGTAAAAGACA+AGG | - | chr4.4:8452458-8452477 | None:intergenic | 30.0% | |
| ATGTAATGTGCAATCTTATC+AGG | + | chr4.4:8450986-8451005 | MS.gene28454:intron | 30.0% | |
| CAATGATGTACTTAACTCAT+GGG | + | chr4.4:8454148-8454167 | MS.gene28454:CDS | 30.0% | |
| CAATTACTCTTGCATAATTC+AGG | + | chr4.4:8454025-8454044 | MS.gene28454:intron | 30.0% | |
| CCTAAGAAACTTAGATCATT+AGG | - | chr4.4:8452056-8452075 | None:intergenic | 30.0% | |
| CCTAATGATCTAAGTTTCTT+AGG | + | chr4.4:8452053-8452072 | MS.gene28454:intron | 30.0% | |
| GTTTCAGAATAGTTAAGAAC+TGG | - | chr4.4:8452342-8452361 | None:intergenic | 30.0% | |
| TCAATACGAGTTTCAATATC+CGG | - | chr4.4:8450620-8450639 | None:intergenic | 30.0% | |
| TCCCACAGATATAGATAAAA+AGG | - | chr4.4:8451817-8451836 | None:intergenic | 30.0% | |
| TGAAAGATAGGATAACATAC+TGG | - | chr4.4:8453418-8453437 | None:intergenic | 30.0% | |
| TGATTTGATGCAAGAAATTG+CGG | + | chr4.4:8450872-8450891 | MS.gene28454:CDS | 30.0% | |
| TTAATCGATTGGTAGGTTTA+GGG | + | chr4.4:8449937-8449956 | MS.gene28454:intron | 30.0% | |
| TTAGTGATGACAGAAATAGT+AGG | + | chr4.4:8451083-8451102 | MS.gene28454:intron | 30.0% | |
| TTTAATCGATTGGTAGGTTT+AGG | + | chr4.4:8449936-8449955 | MS.gene28454:intron | 30.0% | |
| ! | AAATTGATGCGTTTTGTGTT+AGG | + | chr4.4:8449901-8449920 | MS.gene28454:intron | 30.0% |
| ! | AATTGATGCGTTTTGTGTTA+GGG | + | chr4.4:8449902-8449921 | MS.gene28454:intron | 30.0% |
| ! | ATCAGTAGGATTTTCTTTGA+TGG | - | chr4.4:8453160-8453179 | None:intergenic | 30.0% |
| ! | GTGAGAATCGTGATATTTTA+CGG | + | chr4.4:8450516-8450535 | MS.gene28454:intron | 30.0% |
| ! | GTGTAGTTAGAAAGAGTTTT+GGG | + | chr4.4:8450032-8450051 | MS.gene28454:intron | 30.0% |
| ! | TACAACAAGAATGTTCTGAA+AGG | - | chr4.4:8453775-8453794 | None:intergenic | 30.0% |
| ! | TATTTATGGATTGCTAGAGT+TGG | + | chr4.4:8452092-8452111 | MS.gene28454:intron | 30.0% |
| ! | TGTGTAGTTAGAAAGAGTTT+TGG | + | chr4.4:8450031-8450050 | MS.gene28454:intron | 30.0% |
| ! | TGTTTAGAATTCCTTGTCAT+TGG | + | chr4.4:8453471-8453490 | MS.gene28454:intron | 30.0% |
| !! | AATTGTTGTTTGTCTACTGT+TGG | + | chr4.4:8452692-8452711 | MS.gene28454:intron | 30.0% |
| !! | ACTTTTTCTTATAGTGTGTG+GGG | + | chr4.4:8453894-8453913 | MS.gene28454:intron | 30.0% |
| !! | AGGGATAACTTTGGAAATTT+TGG | - | chr4.4:8452285-8452304 | None:intergenic | 30.0% |
| !! | ATAAGTTTTGTGAGACAGTA+TGG | - | chr4.4:8450426-8450445 | None:intergenic | 30.0% |
| !! | CTACATATTTTTCTAGTACC+TGG | + | chr4.4:8452997-8453016 | MS.gene28454:intron | 30.0% |
| !! | CTATGATTTGTTTCTATGCT+TGG | + | chr4.4:8451837-8451856 | MS.gene28454:intron | 30.0% |
| !! | GTTGTTTTCATATGCTATCT+TGG | + | chr4.4:8450334-8450353 | MS.gene28454:intron | 30.0% |
| !! | TAAGTTTTGTGAGACAGTAT+GGG | - | chr4.4:8450425-8450444 | None:intergenic | 30.0% |
| !! | TGTCATAAGCTGTTTTCATA+AGG | + | chr4.4:8450396-8450415 | MS.gene28454:intron | 30.0% |
| !! | TTCAGTTTCATTCACTTTTG+AGG | + | chr4.4:8451528-8451547 | MS.gene28454:intron | 30.0% |
| !! | TTCATTCACTTTTGAGGATA+TGG | + | chr4.4:8451534-8451553 | MS.gene28454:intron | 30.0% |
| AAAACATGTTGCAAGAGTGA+AGG | - | chr4.4:8452721-8452740 | None:intergenic | 35.0% | |
| AAAGAGAATAGAAAGCCTAC+AGG | - | chr4.4:8453189-8453208 | None:intergenic | 35.0% | |
| AACCCAAGTTTAGAAAGCTA+TGG | - | chr4.4:8452908-8452927 | None:intergenic | 35.0% | |
| AAGAACACATTTACAGAGAG+TGG | - | chr4.4:8452503-8452522 | None:intergenic | 35.0% | |
| AAGAAGGTAGGTCCATATAT+CGG | + | chr4.4:8454264-8454283 | MS.gene28454:intron | 35.0% | |
| AAGGAATCCAAATGTTAGGA+AGG | - | chr4.4:8452439-8452458 | None:intergenic | 35.0% | |
| AAGGATGAGAGATGAAAGAT+AGG | - | chr4.4:8453430-8453449 | None:intergenic | 35.0% | |
| ACCCAAGTTTAGAAAGCTAT+GGG | - | chr4.4:8452907-8452926 | None:intergenic | 35.0% | |
| ACTGTTCAGTTCAGATACTA+TGG | + | chr4.4:8450174-8450193 | MS.gene28454:intron | 35.0% | |
| AGACAAGGAATCCAAATGTT+AGG | - | chr4.4:8452443-8452462 | None:intergenic | 35.0% | |
| AGATAGATTTCTATCCTCCA+AGG | - | chr4.4:8453381-8453400 | None:intergenic | 35.0% | |
| AGATCCTGAGGAAAATATGT+TGG | + | chr4.4:8453303-8453322 | MS.gene28454:intron | 35.0% | |
| AGATGAAAAGTAGGGATGAA+AGG | - | chr4.4:8452305-8452324 | None:intergenic | 35.0% | |
| AGCAGACAAAGATGAAAAGT+AGG | - | chr4.4:8452314-8452333 | None:intergenic | 35.0% | |
| AGCCTACTATATACTATCAC+AGG | + | chr4.4:8450547-8450566 | MS.gene28454:intron | 35.0% | |
| AGCCTGTGATAGTATATAGT+AGG | - | chr4.4:8450552-8450571 | None:intergenic | 35.0% | |
| AGGATAACATACTGGAAGTT+TGG | - | chr4.4:8453410-8453429 | None:intergenic | 35.0% | |
| ATATCCTTGGAAAGTGAATG+TGG | + | chr4.4:8453940-8453959 | MS.gene28454:intron | 35.0% | |
| ATTCGACATAGATGCATCTA+AGG | + | chr4.4:8451241-8451260 | MS.gene28454:CDS | 35.0% | |
| CAAGAAACTTGCCAAGATTA+AGG | + | chr4.4:8450821-8450840 | MS.gene28454:CDS | 35.0% | |
| CAAGGTGGAATTTAAAAGAC+TGG | - | chr4.4:8453363-8453382 | None:intergenic | 35.0% | |
| CATATATTGCCTTATAGCAG+CGG | - | chr4.4:8453128-8453147 | None:intergenic | 35.0% | |
| CCTACAGGAATAAAATCAGT+AGG | - | chr4.4:8453174-8453193 | None:intergenic | 35.0% | |
| GAAAAGAAAAAGCCCAAAGA+AGG | + | chr4.4:8451263-8451282 | MS.gene28454:CDS | 35.0% | |
| GACATTAATGCATCACTGTT+AGG | - | chr4.4:8451597-8451616 | None:intergenic | 35.0% | |
| GACCATATTATCACCTTCTT+TGG | - | chr4.4:8451279-8451298 | None:intergenic | 35.0% | |
| GATGAAAAGTAGGGATGAAA+GGG | - | chr4.4:8452304-8452323 | None:intergenic | 35.0% | |
| GCAATGATGTACTTAACTCA+TGG | + | chr4.4:8454147-8454166 | MS.gene28454:CDS | 35.0% | |
| GCAGACAAAGATGAAAAGTA+GGG | - | chr4.4:8452313-8452332 | None:intergenic | 35.0% | |
| GTAGATACTCTAAATTGTCC+TGG | + | chr4.4:8451494-8451513 | MS.gene28454:intron | 35.0% | |
| TAATGTGCAATCTTATCAGG+TGG | + | chr4.4:8450989-8451008 | MS.gene28454:intron | 35.0% | |
| TCAAATACACAAGGAAACCA+TGG | + | chr4.4:8454217-8454236 | MS.gene28454:CDS | 35.0% | |
| TCAGTCCATATCACATATTG+AGG | - | chr4.4:8454449-8454468 | None:intergenic | 35.0% | |
| TTAAATAAGCCACTCCAAAC+AGG | + | chr4.4:8450460-8450479 | MS.gene28454:intron | 35.0% | |
| TTCACTCAAAAACTTTCCAG+AGG | - | chr4.4:8453609-8453628 | None:intergenic | 35.0% | |
| TTTAAAAGACTGGACTGCAT+CGG | - | chr4.4:8453353-8453372 | None:intergenic | 35.0% | |
| ! | AAACCTTCAAAGCTGCTTTA+TGG | - | chr4.4:8454244-8454263 | None:intergenic | 35.0% |
| ! | ACTTGTAGTTACAAGTACTG+TGG | + | chr4.4:8451417-8451436 | MS.gene28454:intron | 35.0% |
| ! | CAGTCTTTTAAATTCCACCT+TGG | + | chr4.4:8453361-8453380 | MS.gene28454:CDS | 35.0% |
| ! | CTTTGAAGGTTTAAGAAGGT+AGG | + | chr4.4:8454252-8454271 | MS.gene28454:intron | 35.0% |
| ! | GGTTTTCTGTCTGTGATTTT+AGG | + | chr4.4:8453067-8453086 | MS.gene28454:intron | 35.0% |
| ! | GTAGGATTTTCTTTGATGGA+CGG | - | chr4.4:8453156-8453175 | None:intergenic | 35.0% |
| ! | TATTTTGCCATTATGCGTTC+AGG | + | chr4.4:8454543-8454562 | MS.gene28454:CDS | 35.0% |
| ! | TCAGATACTATGGTCCTTTT+TGG | + | chr4.4:8450184-8450203 | MS.gene28454:intron | 35.0% |
| ! | TCCTGTTCATTTGAAGTGAA+GGG | + | chr4.4:8452615-8452634 | MS.gene28454:intron | 35.0% |
| ! | TCTTATCAGGTGGAAACTTT+TGG | + | chr4.4:8450999-8451018 | MS.gene28454:intron | 35.0% |
| ! | TCTTTTAAATTCCACCTTGG+AGG | + | chr4.4:8453364-8453383 | MS.gene28454:CDS | 35.0% |
| ! | TTTGGTTCGCTTGAAACTAT+CGG | + | chr4.4:8449961-8449980 | MS.gene28454:intron | 35.0% |
| !! | AAACGACGGTTGATAAGAAT+TGG | - | chr4.4:8452570-8452589 | None:intergenic | 35.0% |
| !! | ATTTAGGGTTTTCCATGCTT+TGG | - | chr4.4:8449825-8449844 | None:intergenic | 35.0% |
| !! | TGCTTATTTCTGGTCATTTC+AGG | + | chr4.4:8454418-8454437 | MS.gene28454:intron | 35.0% |
| !! | TTCTTGAGAGTATTTGTGCA+CGG | - | chr4.4:8451205-8451224 | None:intergenic | 35.0% |
| !! | TTGTTTCTATGCTTGGCATT+TGG | + | chr4.4:8451844-8451863 | MS.gene28454:intron | 35.0% |
| !!! | CCTACTGATTTTATTCCTGT+AGG | + | chr4.4:8453171-8453190 | MS.gene28454:intron | 35.0% |
| !!! | TGAGGATATGGTTTTGATGT+TGG | + | chr4.4:8451546-8451565 | MS.gene28454:intron | 35.0% |
| AAAGTGCACAATTACAGTGC+AGG | - | chr4.4:8452173-8452192 | None:intergenic | 40.0% | |
| AACAGGTACAACCAATGACA+AGG | - | chr4.4:8453485-8453504 | None:intergenic | 40.0% | |
| AACTGAAAACGCAGAAAACC+AGG | - | chr4.4:8451515-8451534 | None:intergenic | 40.0% | |
| ACAAAACTCCTGTCATGACA+AGG | + | chr4.4:8452650-8452669 | MS.gene28454:intron | 40.0% | |
| ACAACAGTATATGGCCTGTT+TGG | - | chr4.4:8450477-8450496 | None:intergenic | 40.0% | |
| ACAGACAGAAAACCAGCATA+AGG | - | chr4.4:8453061-8453080 | None:intergenic | 40.0% | |
| ACATTGAACAGTGTATCTGC+TGG | + | chr4.4:8450650-8450669 | MS.gene28454:CDS | 40.0% | |
| AGGGATGAAAGGGATAACTT+TGG | - | chr4.4:8452294-8452313 | None:intergenic | 40.0% | |
| AGGTGATAATATGGTCGAAG+AGG | + | chr4.4:8451283-8451302 | MS.gene28454:CDS | 40.0% | |
| AGTCTGTTTCCAAACAGCAA+AGG | + | chr4.4:8454121-8454140 | MS.gene28454:CDS | 40.0% | |
| ATAATGGCCTGAACGCATAA+TGG | - | chr4.4:8454553-8454572 | None:intergenic | 40.0% | |
| ATGTTGAGATTGAGAGAGCT+AGG | + | chr4.4:8450793-8450812 | MS.gene28454:CDS | 40.0% | |
| CATTGAACAGTGTATCTGCT+GGG | + | chr4.4:8450651-8450670 | MS.gene28454:CDS | 40.0% | |
| CCAAGATTAAGGAAGAACAG+GGG | + | chr4.4:8450832-8450851 | MS.gene28454:CDS | 40.0% | |
| CCCAAGTTTAGAAAGCTATG+GGG | - | chr4.4:8452906-8452925 | None:intergenic | 40.0% | |
| CCCCATAGCTTTCTAAACTT+GGG | + | chr4.4:8452903-8452922 | MS.gene28454:intron | 40.0% | |
| CTTGGAAAGTGAATGTGGTA+GGG | + | chr4.4:8453945-8453964 | MS.gene28454:intron | 40.0% | |
| GAACGAGTTTGACAATGAGA+TGG | + | chr4.4:8453583-8453602 | MS.gene28454:CDS | 40.0% | |
| GAGCTGTCATCAAATACACA+AGG | + | chr4.4:8454208-8454227 | MS.gene28454:CDS | 40.0% | |
| GAGGTGTGTAATGCTAGTAA+TGG | + | chr4.4:8453855-8453874 | MS.gene28454:intron | 40.0% | |
| GAGTAAAGAATTGGTCACAG+TGG | + | chr4.4:8452860-8452879 | MS.gene28454:intron | 40.0% | |
| GCAAAGATTGACAGACCAAT+GGG | + | chr4.4:8454095-8454114 | MS.gene28454:CDS | 40.0% | |
| GCAATATATTGACGAGACAC+CGG | + | chr4.4:8450598-8450617 | MS.gene28454:CDS | 40.0% | |
| GCATCTATGTCGAATACTCT+TGG | - | chr4.4:8451236-8451255 | None:intergenic | 40.0% | |
| GCCAAGATTAAGGAAGAACA+GGG | + | chr4.4:8450831-8450850 | MS.gene28454:CDS | 40.0% | |
| GCCCAAAGAAGGTGATAATA+TGG | + | chr4.4:8451274-8451293 | MS.gene28454:CDS | 40.0% | |
| GTCATGACAAGGTTTAGGAA+AGG | + | chr4.4:8452661-8452680 | MS.gene28454:intron | 40.0% | |
| GTCCATATCACATATTGAGG+AGG | - | chr4.4:8454446-8454465 | None:intergenic | 40.0% | |
| GTCTGATATGGTTGTTACCA+AGG | + | chr4.4:8454064-8454083 | MS.gene28454:CDS | 40.0% | |
| TACTAGCATTACACACCTCT+AGG | - | chr4.4:8453854-8453873 | None:intergenic | 40.0% | |
| TAGATTTCTATCCTCCAAGG+TGG | - | chr4.4:8453378-8453397 | None:intergenic | 40.0% | |
| TATCTACCCTGAACGCATAA+TGG | - | chr4.4:8454569-8454588 | None:intergenic | 40.0% | |
| TCCCCATAGCTTTCTAAACT+TGG | + | chr4.4:8452902-8452921 | MS.gene28454:intron | 40.0% | |
| TCTTGCATATACAGATCCTG+AGG | + | chr4.4:8453291-8453310 | MS.gene28454:intron | 40.0% | |
| TGAAATGTTGCTCACTGTGT+TGG | + | chr4.4:8454493-8454512 | MS.gene28454:CDS | 40.0% | |
| TGAAATTTGCCGCTGCTATA+AGG | + | chr4.4:8453116-8453135 | MS.gene28454:CDS | 40.0% | |
| TGCAAAGATTGACAGACCAA+TGG | + | chr4.4:8454094-8454113 | MS.gene28454:CDS | 40.0% | |
| TGCCAAGATTAAGGAAGAAC+AGG | + | chr4.4:8450830-8450849 | MS.gene28454:CDS | 40.0% | |
| TGCCTCCTCAATATGTGATA+TGG | + | chr4.4:8454441-8454460 | MS.gene28454:CDS | 40.0% | |
| TGGAAACAGACTATTCCCAT+TGG | - | chr4.4:8454113-8454132 | None:intergenic | 40.0% | |
| TGGTCATCCTTCCTAACATT+TGG | + | chr4.4:8452429-8452448 | MS.gene28454:intron | 40.0% | |
| TGTTTCAACTCCAGTAGAGA+CGG | - | chr4.4:8451323-8451342 | None:intergenic | 40.0% | |
| TTCAAATGAGCATCCCCATA+AGG | - | chr4.4:8454477-8454496 | None:intergenic | 40.0% | |
| TTCCTAAACCTTGTCATGAC+AGG | - | chr4.4:8452661-8452680 | None:intergenic | 40.0% | |
| TTGAGAAAGGAGCCGATATA+TGG | - | chr4.4:8454279-8454298 | None:intergenic | 40.0% | |
| TTGGAAAGTGAATGTGGTAG+GGG | + | chr4.4:8453946-8453965 | MS.gene28454:intron | 40.0% | |
| ! | AGCACCAACATATTTTCCTC+AGG | - | chr4.4:8453310-8453329 | None:intergenic | 40.0% |
| ! | ATGCGTTTTGTGTTAGGGTT+TGG | + | chr4.4:8449907-8449926 | MS.gene28454:intron | 40.0% |
| ! | ATGGACTGAAGTTGCCTTAT+GGG | + | chr4.4:8454460-8454479 | MS.gene28454:CDS | 40.0% |
| ! | GAGGAAAATATGTTGGTGCT+TGG | + | chr4.4:8453310-8453329 | MS.gene28454:CDS | 40.0% |
| ! | GCACTGTAATTGTGCACTTT+TGG | + | chr4.4:8452173-8452192 | MS.gene28454:intron | 40.0% |
| ! | GTCCTGTTCATTTGAAGTGA+AGG | + | chr4.4:8452614-8452633 | MS.gene28454:intron | 40.0% |
| ! | TATGGACTGAAGTTGCCTTA+TGG | + | chr4.4:8454459-8454478 | MS.gene28454:CDS | 40.0% |
| ! | TGCGTTTTGTGTTAGGGTTT+GGG | + | chr4.4:8449908-8449927 | MS.gene28454:intron | 40.0% |
| ! | TTATGGATTGCTAGAGTTGG+TGG | + | chr4.4:8452095-8452114 | MS.gene28454:intron | 40.0% |
| !! | ACTTTAGAGCTGTCAAACTC+TGG | + | chr4.4:8452403-8452422 | MS.gene28454:intron | 40.0% |
| !! | CATTGCTATCCTTTGCTGTT+TGG | - | chr4.4:8454133-8454152 | None:intergenic | 40.0% |
| !! | GATTGGTAGGTTTAGGGTTT+TGG | + | chr4.4:8449943-8449962 | MS.gene28454:intron | 40.0% |
| !! | GCAGCTTTGAAGGTTTAAGA+AGG | + | chr4.4:8454248-8454267 | MS.gene28454:intron | 40.0% |
| !! | GTTGTTTTGTCTAAACGACG+CGG | + | chr4.4:8449854-8449873 | MS.gene28454:CDS | 40.0% |
| !! | TGAGAAGCATCTGTCTGATA+TGG | + | chr4.4:8454052-8454071 | MS.gene28454:CDS | 40.0% |
| !!! | TCTGTTTTGAAGCCAAAGCA+TGG | + | chr4.4:8449810-8449829 | MS.gene28454:CDS | 40.0% |
| AATCTTTGCAACCAGAGCCT+TGG | - | chr4.4:8454084-8454103 | None:intergenic | 45.0% | |
| AGTAGAGACGGAATATCTGC+AGG | - | chr4.4:8451311-8451330 | None:intergenic | 45.0% | |
| AGTATATGGCCTGTTTGGAG+TGG | - | chr4.4:8450472-8450491 | None:intergenic | 45.0% | |
| ATCACAGGCTGTAACAGCTA+TGG | + | chr4.4:8450562-8450581 | MS.gene28454:intron | 45.0% | |
| CATCCAATGGACAGCTCTTT+GGG | + | chr4.4:8453550-8453569 | MS.gene28454:CDS | 45.0% | |
| CCCCTGTTCTTCCTTAATCT+TGG | - | chr4.4:8450835-8450854 | None:intergenic | 45.0% | |
| CCTACCACATTCACTTTCCA+AGG | - | chr4.4:8453947-8453966 | None:intergenic | 45.0% | |
| CCTTGGAAAGTGAATGTGGT+AGG | + | chr4.4:8453944-8453963 | MS.gene28454:intron | 45.0% | |
| CGATTTCACAGTCAGTGAAG+TGG | - | chr4.4:8452943-8452962 | None:intergenic | 45.0% | |
| CTCCTGTCATGACAAGGTTT+AGG | + | chr4.4:8452656-8452675 | MS.gene28454:intron | 45.0% | |
| GAGCTGTCAAACTCTGGTTA+TGG | + | chr4.4:8452409-8452428 | MS.gene28454:intron | 45.0% | |
| GCCCTTCACTTCAAATGAAC+AGG | - | chr4.4:8452619-8452638 | None:intergenic | 45.0% | |
| GGAGAAACTGCTTGATCTTG+TGG | + | chr4.4:8454181-8454200 | MS.gene28454:CDS | 45.0% | |
| TAACTCATGGGCAGCAAACT+TGG | + | chr4.4:8454160-8454179 | MS.gene28454:CDS | 45.0% | |
| TATGGTTGTTACCAAGGCTC+TGG | + | chr4.4:8454070-8454089 | MS.gene28454:CDS | 45.0% | |
| TCACCATGGAAGTCATCCAA+TGG | + | chr4.4:8453537-8453556 | MS.gene28454:CDS | 45.0% | |
| TCATCCAATGGACAGCTCTT+TGG | + | chr4.4:8453549-8453568 | MS.gene28454:CDS | 45.0% | |
| TGAATGTGGTAGGGGAATTG+TGG | + | chr4.4:8453954-8453973 | MS.gene28454:intron | 45.0% | |
| TGAGATTGAGAGAGCTAGGT+TGG | + | chr4.4:8450797-8450816 | MS.gene28454:CDS | 45.0% | |
| TGCAGATATTCCGTCTCTAC+TGG | + | chr4.4:8451310-8451329 | MS.gene28454:CDS | 45.0% | |
| TGGACTGAAGTTGCCTTATG+GGG | + | chr4.4:8454461-8454480 | MS.gene28454:CDS | 45.0% | |
| TGGTCACAGTGGAGTATATG+TGG | + | chr4.4:8452871-8452890 | MS.gene28454:intron | 45.0% | |
| TGGTTCCATAGACGAAAGTG+CGG | + | chr4.4:8451864-8451883 | MS.gene28454:intron | 45.0% | |
| TGTAACAGCTATGGTACAGC+AGG | + | chr4.4:8450571-8450590 | MS.gene28454:CDS | 45.0% | |
| TGTACCTGTTACACGTGTCT+AGG | + | chr4.4:8453495-8453514 | MS.gene28454:intron | 45.0% | |
| TGTCCATTGGATGACTTCCA+TGG | - | chr4.4:8453543-8453562 | None:intergenic | 45.0% | |
| TTCAGGCCATTATGCGTTCA+GGG | + | chr4.4:8454560-8454579 | MS.gene28454:CDS | 45.0% | |
| TTTGACAATGAGATGGCCTC+TGG | + | chr4.4:8453590-8453609 | MS.gene28454:CDS | 45.0% | |
| TTTGTGCACGGACATAATCC+TGG | - | chr4.4:8451193-8451212 | None:intergenic | 45.0% | |
| ! | ATTTGAAGTGAAGGGCAACC+AGG | + | chr4.4:8452623-8452642 | MS.gene28454:intron | 45.0% |
| ! | CAAAGCTGCTTTATGGACCA+TGG | - | chr4.4:8454237-8454256 | None:intergenic | 45.0% |
| ! | CGGATTGGAGTACATGTTTG+TGG | + | chr4.4:8451884-8451903 | MS.gene28454:intron | 45.0% |
| ! | GGTCCATAAAGCAGCTTTGA+AGG | + | chr4.4:8454238-8454257 | MS.gene28454:CDS | 45.0% |
| !! | GGTGGAAACTTTTGGTGCAA+TGG | + | chr4.4:8451007-8451026 | MS.gene28454:intron | 45.0% |
| !!! | TAGGCTACTTTTGAAGCAGC+TGG | + | chr4.4:8453514-8453533 | MS.gene28454:intron | 45.0% |
| AGAACAGGGGCTTATAGCTG+AGG | + | chr4.4:8450845-8450864 | MS.gene28454:CDS | 50.0% | |
| CCAATCCGCACTTTCGTCTA+TGG | - | chr4.4:8451872-8451891 | None:intergenic | 50.0% | |
| GAACAGTGTATCTGCTGGGA+AGG | + | chr4.4:8450655-8450674 | MS.gene28454:CDS | 50.0% | |
| GAGAAACAAATGCGTCTCGC+CGG | + | chr4.4:8449743-8449762 | MS.gene28454:CDS | 50.0% | |
| GTAGCCTAGACACGTGTAAC+AGG | - | chr4.4:8453502-8453521 | None:intergenic | 50.0% | |
| GTTCAGGCCATTATGCGTTC+AGG | + | chr4.4:8454559-8454578 | MS.gene28454:CDS | 50.0% | |
| TCTCCCAAAGAGCTGTCCAT+TGG | - | chr4.4:8453556-8453575 | None:intergenic | 50.0% | |
| ! | CCATAGACGAAAGTGCGGAT+TGG | + | chr4.4:8451869-8451888 | MS.gene28454:intron | 50.0% |
| ! | GCTTTTGTGTCTCAGCCTAG+AGG | + | chr4.4:8453836-8453855 | MS.gene28454:CDS | 50.0% |
| ! | TGTTGCTCACTGTGTTGGTG+AGG | + | chr4.4:8454498-8454517 | MS.gene28454:CDS | 50.0% |
| ! | TTTGAAGCAGCTGGTCACCA+TGG | + | chr4.4:8453523-8453542 | MS.gene28454:CDS | 50.0% |
| !! | TCTAGTGCCGACTATTTCGC+CGG | - | chr4.4:8449765-8449784 | None:intergenic | 50.0% |
| GCAGTCGGACAGACAGTCAT+TGG | - | chr4.4:8451661-8451680 | None:intergenic | 55.0% | |
| GGACATAATCCTGGCGGTCT+AGG | - | chr4.4:8451184-8451203 | None:intergenic | 55.0% | |
| GTGCACGGACATAATCCTGG+CGG | - | chr4.4:8451190-8451209 | None:intergenic | 55.0% | |
| ! | CTGGACTGCATCGGTGAATG+TGG | - | chr4.4:8453344-8453363 | None:intergenic | 55.0% |
| !! | GACAGGAGTTTTGTAGCGCC+TGG | - | chr4.4:8452644-8452663 | None:intergenic | 55.0% |
| CGTCTCGCCGGCGAAATAGT+CGG | + | chr4.4:8449755-8449774 | MS.gene28454:CDS | 60.0% | |
| TCGATTGTGCCTAGACCGCC+AGG | + | chr4.4:8451172-8451191 | MS.gene28454:CDS | 60.0% | |
| ACGACGCGGTCAGCTCAAGC+AGG | + | chr4.4:8449868-8449887 | MS.gene28454:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 8449701 | 8454637 | 8449701 | ID=MS.gene28454 |
| chr4.4 | mRNA | 8449701 | 8454637 | 8449701 | ID=MS.gene28454.t1;Parent=MS.gene28454 |
| chr4.4 | exon | 8449701 | 8449889 | 8449701 | ID=MS.gene28454.t1.exon1;Parent=MS.gene28454.t1 |
| chr4.4 | CDS | 8449701 | 8449889 | 8449701 | ID=cds.MS.gene28454.t1;Parent=MS.gene28454.t1 |
| chr4.4 | exon | 8450569 | 8450676 | 8450569 | ID=MS.gene28454.t1.exon2;Parent=MS.gene28454.t1 |
| chr4.4 | CDS | 8450569 | 8450676 | 8450569 | ID=cds.MS.gene28454.t1;Parent=MS.gene28454.t1 |
| chr4.4 | exon | 8450789 | 8450893 | 8450789 | ID=MS.gene28454.t1.exon3;Parent=MS.gene28454.t1 |
| chr4.4 | CDS | 8450789 | 8450893 | 8450789 | ID=cds.MS.gene28454.t1;Parent=MS.gene28454.t1 |
| chr4.4 | exon | 8451008 | 8451064 | 8451008 | ID=MS.gene28454.t1.exon4;Parent=MS.gene28454.t1 |
| chr4.4 | CDS | 8451008 | 8451064 | 8451008 | ID=cds.MS.gene28454.t1;Parent=MS.gene28454.t1 |
| chr4.4 | exon | 8451170 | 8451366 | 8451170 | ID=MS.gene28454.t1.exon5;Parent=MS.gene28454.t1 |
| chr4.4 | CDS | 8451170 | 8451366 | 8451170 | ID=cds.MS.gene28454.t1;Parent=MS.gene28454.t1 |
| chr4.4 | exon | 8453089 | 8453188 | 8453089 | ID=MS.gene28454.t1.exon6;Parent=MS.gene28454.t1 |
| chr4.4 | CDS | 8453089 | 8453188 | 8453089 | ID=cds.MS.gene28454.t1;Parent=MS.gene28454.t1 |
| chr4.4 | exon | 8453305 | 8453417 | 8453305 | ID=MS.gene28454.t1.exon7;Parent=MS.gene28454.t1 |
| chr4.4 | CDS | 8453305 | 8453417 | 8453305 | ID=cds.MS.gene28454.t1;Parent=MS.gene28454.t1 |
| chr4.4 | exon | 8453517 | 8453667 | 8453517 | ID=MS.gene28454.t1.exon8;Parent=MS.gene28454.t1 |
| chr4.4 | CDS | 8453517 | 8453667 | 8453517 | ID=cds.MS.gene28454.t1;Parent=MS.gene28454.t1 |
| chr4.4 | exon | 8453780 | 8453857 | 8453780 | ID=MS.gene28454.t1.exon9;Parent=MS.gene28454.t1 |
| chr4.4 | CDS | 8453780 | 8453857 | 8453780 | ID=cds.MS.gene28454.t1;Parent=MS.gene28454.t1 |
| chr4.4 | exon | 8454047 | 8454259 | 8454047 | ID=MS.gene28454.t1.exon10;Parent=MS.gene28454.t1 |
| chr4.4 | CDS | 8454047 | 8454259 | 8454047 | ID=cds.MS.gene28454.t1;Parent=MS.gene28454.t1 |
| chr4.4 | exon | 8454440 | 8454637 | 8454440 | ID=MS.gene28454.t1.exon11;Parent=MS.gene28454.t1 |
| chr4.4 | CDS | 8454440 | 8454637 | 8454440 | ID=cds.MS.gene28454.t1;Parent=MS.gene28454.t1 |
| Gene Sequence |
| Protein sequence |
