AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32974

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32974.t1 MTR_1g016750 100.000 267 0 0 1 267 1 267 0.0 548
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32974.t1 AT1G64520 83.521 267 44 0 1 267 1 267 4.37e-159 442
MS.gene32974.t1 AT5G42040 62.921 267 65 2 1 267 1 233 6.97e-113 324

Find 67 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGAGAAACTTCTGTGAATT+TGG 0.038934 1.1:+5660319 None:intergenic
CCGTTCTTGATTTCCCATTC+AGG 0.264023 1.1:+5656532 None:intergenic
CACTTGCAGGTGTTACTAAC+AGG 0.267676 1.1:-5659124 MS.gene32974:intron
GGAGTTGGAGCAATCCTTTA+TGG 0.271340 1.1:-5657284 MS.gene32974:CDS
TGTGTATTTCATGGATCTTT+TGG 0.284808 1.1:-5657206 MS.gene32974:CDS
TTCCAAGAGTTCCTGGTCTT+TGG 0.286082 1.1:+5656751 None:intergenic
GTTAGCTCTTGAACTGCATT+AGG 0.332972 1.1:+5659067 None:intergenic
GGAACTCTTGGAATATATTA+AGG 0.340067 1.1:-5656741 MS.gene32974:CDS
TGCTGAATTCCATACTGAAT+TGG 0.350776 1.1:-5657356 MS.gene32974:CDS
TGAGAAACTTCTGTGAATTT+GGG 0.351752 1.1:+5660320 None:intergenic
CCCTAGCATAACTAAGTGTT+TGG 0.361635 1.1:+5656440 None:intergenic
TAATATATTCCAAGAGTTCC+TGG 0.370117 1.1:+5656744 None:intergenic
TGGGTACTCCTGAGGAGATT+GGG 0.370608 1.1:+5657423 None:intergenic
GTGTTTGGTTGATGAGTTGC+AGG 0.382114 1.1:+5656455 None:intergenic
TGAGACTTCTTGTGCAGAAT+AGG 0.396052 1.1:-5657381 MS.gene32974:CDS
CGTTCTTGATTTCCCATTCA+GGG 0.400779 1.1:+5656533 None:intergenic
TGAAACATATGTGTATTTCA+TGG 0.407221 1.1:-5657215 MS.gene32974:CDS
AAAGCAATTCCAATTCAGTA+TGG 0.408550 1.1:+5657347 None:intergenic
TTGGGTACTCCTGAGGAGAT+TGG 0.426130 1.1:+5657422 None:intergenic
CACCAAAGACCAGGAACTCT+TGG 0.427127 1.1:-5656753 MS.gene32974:CDS
TACCGTGCATCAGTGTAGTA+AGG 0.430307 1.1:+5657702 None:intergenic
ATTGCTTTCATCCACTGCTC+TGG 0.436359 1.1:-5657332 MS.gene32974:CDS
GTTGTATGCCCCTTCCATAA+AGG 0.451259 1.1:+5657270 None:intergenic
CCTCAGGAGTACCCAATACT+TGG 0.457349 1.1:-5657415 MS.gene32974:CDS
ACAGGTTGAGACCAAGTATT+GGG 0.478577 1.1:+5657404 None:intergenic
TCTGCACAAGAAGTCTCAAC+AGG 0.487747 1.1:+5657386 None:intergenic
ACTTAGTTATGCTAGGGAGT+TGG 0.495838 1.1:-5656434 MS.gene32974:CDS
GGTTAGAGTGTACCTTGAGC+TGG 0.497473 1.1:+5660231 None:intergenic
TATTAAGCATGCCGTGGAGT+TGG 0.497949 1.1:-5657299 MS.gene32974:CDS
GATTTCAGGAGCACCCTGAA+TGG 0.499374 1.1:-5656546 MS.gene32974:intron
ATTTCAGGAGCACCCTGAAT+GGG 0.504260 1.1:-5656545 MS.gene32974:intron
TCGTCTTCCCCAATCTCCTC+AGG 0.513848 1.1:-5657431 MS.gene32974:CDS
AGGGAGGGTATTTCTTTGCA+AGG 0.516901 1.1:+5656475 None:intergenic
TTGAAAGAAATCTCTTTCAA+AGG 0.524033 1.1:+5657731 None:intergenic
TGGTTGATGAGTTGCAGGGA+GGG 0.525378 1.1:+5656460 None:intergenic
GGAGCAATCCTTTATGGAAG+GGG 0.535947 1.1:-5657278 MS.gene32974:CDS
TTGGAGCAATCCTTTATGGA+AGG 0.551519 1.1:-5657280 MS.gene32974:CDS
ATTAGGTGTATCTGCAAACA+AGG 0.554484 1.1:+5659084 None:intergenic
AACAGGTTGAGACCAAGTAT+TGG 0.562540 1.1:+5657403 None:intergenic
GAATCTCCTTTCCCAGCTCA+AGG 0.562925 1.1:-5660243 MS.gene32974:intron
TGGAGCAATCCTTTATGGAA+GGG 0.575444 1.1:-5657279 MS.gene32974:CDS
ACGTTCAGAGATGAGATAGC+AGG 0.577525 1.1:-5656836 MS.gene32974:intron
AGTGTACCTTGAGCTGGGAA+AGG 0.581779 1.1:+5660237 None:intergenic
GATAGCAGGATGCAGTGAGA+AGG 0.587670 1.1:-5656822 MS.gene32974:CDS
CCAAACACTTAGTTATGCTA+GGG 0.588753 1.1:-5656440 MS.gene32974:CDS
GTTAGAGTGTACCTTGAGCT+GGG 0.589939 1.1:+5660232 None:intergenic
TATCTGCAAACAAGGGTGGA+AGG 0.590332 1.1:+5659092 None:intergenic
GGGTACTCCTGAGGAGATTG+GGG 0.592535 1.1:+5657424 None:intergenic
ACCAAACACTTAGTTATGCT+AGG 0.593116 1.1:-5656441 MS.gene32974:CDS
TCCACGGCATGCTTAATACA+AGG 0.594862 1.1:+5657304 None:intergenic
TTGGTTGATGAGTTGCAGGG+AGG 0.604694 1.1:+5656459 None:intergenic
TGTTTGGTTGATGAGTTGCA+GGG 0.615391 1.1:+5656456 None:intergenic
GGTGTATCTGCAAACAAGGG+TGG 0.618117 1.1:+5659088 None:intergenic
ACAAGGATTCTCCAGAGCAG+TGG 0.619513 1.1:+5657321 None:intergenic
AAAGGATTGCTCCAACTCCA+CGG 0.630188 1.1:+5657288 None:intergenic
GTTATGCTAGGGAGTTGGAG+AGG 0.631284 1.1:-5656429 MS.gene32974:CDS
ACTTGCAGGTGTTACTAACA+GGG 0.633305 1.1:-5659123 MS.gene32974:intron
AACCTTACTACACTGATGCA+CGG 0.640807 1.1:-5657704 MS.gene32974:intron
TCTGTCGAGCACTCAAGACA+CGG 0.643446 1.1:+5657248 None:intergenic
TCCTTGTATTAAGCATGCCG+TGG 0.644930 1.1:-5657305 MS.gene32974:CDS
CCAAGTATTGGGTACTCCTG+AGG 0.648239 1.1:+5657415 None:intergenic
GTTGCTGTTCACCAAAGACC+AGG 0.651601 1.1:-5656762 MS.gene32974:CDS
AAATACACATATGTTTCATG+TGG 0.652663 1.1:+5657220 None:intergenic
ACTCTTGGAATATATTAAGG+AGG 0.657443 1.1:-5656738 MS.gene32974:intron
CCTGAATGGGAAATCAAGAA+CGG 0.657945 1.1:-5656532 MS.gene32974:CDS
CTGGGAAAGGAGATTCGAAG+CGG 0.699565 1.1:+5660250 None:intergenic
TTAGGTGTATCTGCAAACAA+GGG 0.724231 1.1:+5659085 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTAAAAAAAAAAATA+CGG + chr1.1:5657840-5657859 None:intergenic 0.0%
!! AAGTAAAAAAAATAAATAGA+TGG + chr1.1:5659650-5659669 None:intergenic 10.0%
!! ATTTGTATAAAAAAATTAAG+AGG - chr1.1:5656654-5656673 MS.gene32974:intron 10.0%
!! TATATGTATATTACTAAAAT+AGG + chr1.1:5658350-5658369 None:intergenic 10.0%
!! TTTGTATAAAAAAATTAAGA+GGG - chr1.1:5656655-5656674 MS.gene32974:intron 10.0%
!!! ATAGTTTTTATTTCTAAAAT+TGG - chr1.1:5658125-5658144 MS.gene32974:intron 10.0%
!!! TTTATTTTGTATAATTGATA+TGG + chr1.1:5658799-5658818 None:intergenic 10.0%
!! AATCTTAAATTAAATTAGGT+TGG - chr1.1:5658563-5658582 MS.gene32974:intron 15.0%
!! AATTATTTCATAATCAATTC+TGG - chr1.1:5658495-5658514 MS.gene32974:intron 15.0%
!! AGTGAATCTTAAATTAAATT+AGG - chr1.1:5658559-5658578 MS.gene32974:intron 15.0%
!! ATTACTAATAACACAAAATA+CGG + chr1.1:5658282-5658301 None:intergenic 15.0%
!! TTGTATAAAAAAATTAAGAG+GGG - chr1.1:5656656-5656675 MS.gene32974:intron 15.0%
!!! AAGAGTTTTTATACTAAAAT+TGG - chr1.1:5658724-5658743 MS.gene32974:intron 15.0%
!!! CACTAAAATCTTTTATTTTT+AGG + chr1.1:5657119-5657138 None:intergenic 15.0%
!!! CTAAAAATAAAAGATTTTAG+TGG - chr1.1:5657117-5657136 MS.gene32974:intron 15.0%
!!! CTAAAATATGTTTCTTTTAT+AGG + chr1.1:5656882-5656901 None:intergenic 15.0%
!!! TAACATAATTGATTTATGTT+TGG + chr1.1:5658439-5658458 None:intergenic 15.0%
!!! TATTGTAAATGACATTTTAA+TGG - chr1.1:5657909-5657928 MS.gene32974:intron 15.0%
!! AAATTCTCGACAAATAATTA+TGG + chr1.1:5657507-5657526 None:intergenic 20.0%
!! AGATATACTAATTATAGAAC+CGG + chr1.1:5660177-5660196 None:intergenic 20.0%
!! ATATGAGTGAATCTTAAATT+AGG - chr1.1:5657883-5657902 MS.gene32974:intron 20.0%
!! CAAAGAAAAAGTAAAAATAC+AGG + chr1.1:5657791-5657810 None:intergenic 20.0%
!! TAATAAACTCAGTTATTGTA+GGG - chr1.1:5657462-5657481 MS.gene32974:intron 20.0%
!! TGTATAATTGATATGGAAAA+TGG + chr1.1:5658792-5658811 None:intergenic 20.0%
!! TGTGTTAATTTATGATGTTT+GGG + chr1.1:5658395-5658414 None:intergenic 20.0%
!! TTAATAAACTCAGTTATTGT+AGG - chr1.1:5657461-5657480 MS.gene32974:intron 20.0%
!! TTATGGAAAACTAAATGATA+AGG + chr1.1:5657490-5657509 None:intergenic 20.0%
!! TTGTGTTAATTTATGATGTT+TGG + chr1.1:5658396-5658415 None:intergenic 20.0%
!!! AAAAGAAACATATTTTAGAG+AGG - chr1.1:5656884-5656903 MS.gene32974:intron 20.0%
!!! ATTTTTTTTACTTCTGAAGT+TGG - chr1.1:5659657-5659676 MS.gene32974:intron 20.0%
!!! GATAGATAAATTTTTCTATC+TGG - chr1.1:5658628-5658647 MS.gene32974:intron 20.0%
! AAATACACATATGTTTCATG+TGG + chr1.1:5659529-5659548 None:intergenic 25.0%
! AACACACAACATTGAAATTA+GGG + chr1.1:5656566-5656585 None:intergenic 25.0%
! AATTAATATGATCGAGTCAT+GGG + chr1.1:5658838-5658857 None:intergenic 25.0%
! ATAACCTAATGACCTAAATA+AGG - chr1.1:5656768-5656787 MS.gene32974:CDS 25.0%
! CACTCATATGATATTTGATA+AGG + chr1.1:5657872-5657891 None:intergenic 25.0%
! CTACAAGGTAATATACTATA+AGG + chr1.1:5659176-5659195 None:intergenic 25.0%
! GATATTCTTGTGAAGTAAAA+TGG - chr1.1:5656927-5656946 MS.gene32974:intron 25.0%
! TAATTAATATGATCGAGTCA+TGG + chr1.1:5658839-5658858 None:intergenic 25.0%
! TCATATGATATTTGATAAGG+TGG + chr1.1:5657869-5657888 None:intergenic 25.0%
! TGAAACATATGTGTATTTCA+TGG - chr1.1:5659531-5659550 MS.gene32974:intron 25.0%
! TGTTGAGCTATTGTAATTTA+TGG - chr1.1:5659269-5659288 MS.gene32974:intron 25.0%
! TTATAATAATGGTTGTGCTT+CGG + chr1.1:5659805-5659824 None:intergenic 25.0%
! TTGAAAGAAATCTCTTTCAA+AGG + chr1.1:5659018-5659037 None:intergenic 25.0%
!! ATTGTGTACTTGTTAACAAA+GGG + chr1.1:5659082-5659101 None:intergenic 25.0%
!! CTTCTTGTTGAAAATTTTCA+GGG - chr1.1:5658940-5658959 MS.gene32974:intron 25.0%
!! CTTGATACTTGTTAGTTATT+GGG - chr1.1:5657225-5657244 MS.gene32974:CDS 25.0%
!! TATTGTGTACTTGTTAACAA+AGG + chr1.1:5659083-5659102 None:intergenic 25.0%
!! TCTTCTTGTTGAAAATTTTC+AGG - chr1.1:5658939-5658958 MS.gene32974:intron 25.0%
!! TCTTGATACTTGTTAGTTAT+TGG - chr1.1:5657224-5657243 MS.gene32974:CDS 25.0%
!! TTCTTGTTGAAAATTTTCAG+GGG - chr1.1:5658941-5658960 MS.gene32974:intron 25.0%
!!! AAATAAAAGATTTTAGTGGC+AGG - chr1.1:5657121-5657140 MS.gene32974:intron 25.0%
!!! AAATTAAATTAGGTTGGAAG+AGG - chr1.1:5658569-5658588 MS.gene32974:intron 25.0%
!!! TGACAAATACTGTATTTTAG+AGG - chr1.1:5658689-5658708 MS.gene32974:intron 25.0%
!!! TTAGTATATCTACTGATTTC+AGG - chr1.1:5660186-5660205 MS.gene32974:intron 25.0%
AAAACACCATAGAAACTGAA+TGG + chr1.1:5657728-5657747 None:intergenic 30.0%
AAACTTTGTCTAACCGTTTA+AGG + chr1.1:5657387-5657406 None:intergenic 30.0%
AAAGAAACTAGGGTTTAGTA+GGG + chr1.1:5656539-5656558 None:intergenic 30.0%
AAAGCAATTCCAATTCAGTA+TGG + chr1.1:5659402-5659421 None:intergenic 30.0%
AACTAGTTAGCAAATTGACA+TGG + chr1.1:5659879-5659898 None:intergenic 30.0%
ACATAAGTGTTTGTGAGAAA+CGG - chr1.1:5657022-5657041 MS.gene32974:intron 30.0%
ACTCTTGGAATATATTAAGG+AGG - chr1.1:5660008-5660027 MS.gene32974:intron 30.0%
AGATAGATAACTGTTCTATC+TGG - chr1.1:5658153-5658172 MS.gene32974:intron 30.0%
AGCAATAGTTTCCTTTCTAT+TGG + chr1.1:5657190-5657209 None:intergenic 30.0%
ATCTATCTTTCTTACTACCA+TGG + chr1.1:5658615-5658634 None:intergenic 30.0%
ATTGAACTCAACCGTTAAAT+TGG - chr1.1:5659620-5659639 MS.gene32974:intron 30.0%
CAACACACAACATTGAAATT+AGG + chr1.1:5656567-5656586 None:intergenic 30.0%
CAAGAAAGCAAATAATGAGT+TGG + chr1.1:5659251-5659270 None:intergenic 30.0%
CTAATGACCTAAATAAGGAA+CGG - chr1.1:5656773-5656792 MS.gene32974:CDS 30.0%
GAGCTTCAAGGTTATAATAA+TGG + chr1.1:5659816-5659835 None:intergenic 30.0%
GATAATACTTTCACGTTTCT+TGG + chr1.1:5659205-5659224 None:intergenic 30.0%
GCAATAGTTTCCTTTCTATT+GGG + chr1.1:5657189-5657208 None:intergenic 30.0%
GGAAAAACAATATACAACAG+AGG + chr1.1:5659844-5659863 None:intergenic 30.0%
GGAACTCTTGGAATATATTA+AGG - chr1.1:5660005-5660024 MS.gene32974:intron 30.0%
GTTTCTTGGTATATACTACA+AGG + chr1.1:5659191-5659210 None:intergenic 30.0%
TAAGATAATAACTGCTTGTC+AGG - chr1.1:5658866-5658885 MS.gene32974:intron 30.0%
TAATATATTCCAAGAGTTCC+TGG + chr1.1:5660005-5660024 None:intergenic 30.0%
TAGATGGAGTTCCAATTTAA+CGG + chr1.1:5659634-5659653 None:intergenic 30.0%
TAGTTCCTGCAATGAAAATT+TGG - chr1.1:5659779-5659798 MS.gene32974:intron 30.0%
TGACAACAATTATTACCTGA+TGG + chr1.1:5659572-5659591 None:intergenic 30.0%
TGAGAAACTTCTGTGAATTT+GGG + chr1.1:5656429-5656448 None:intergenic 30.0%
TTAAAATAGCACCTAATTGC+TGG - chr1.1:5657548-5657567 MS.gene32974:intron 30.0%
TTCATTCCATTCAGTTTCTA+TGG - chr1.1:5657719-5657738 MS.gene32974:CDS 30.0%
TTCCTTTCTATTGGGTATTA+GGG + chr1.1:5657181-5657200 None:intergenic 30.0%
TTGTGAAGTAAAATGGTTGT+GGG - chr1.1:5656934-5656953 MS.gene32974:intron 30.0%
TTGTTAGTTATTGGGCTTAT+AGG - chr1.1:5657233-5657252 MS.gene32974:CDS 30.0%
TTTAAAGACTATAGCCAGTT+TGG + chr1.1:5657533-5657552 None:intergenic 30.0%
TTTCCTTTCTATTGGGTATT+AGG + chr1.1:5657182-5657201 None:intergenic 30.0%
TTTCTCACAAACACTTATGT+GGG + chr1.1:5657022-5657041 None:intergenic 30.0%
! AATTTTTACACTAAGGACGA+CGG + chr1.1:5658982-5659001 None:intergenic 30.0%
! GATCCTCAATTTTTACACTA+AGG + chr1.1:5658989-5659008 None:intergenic 30.0%
! TGAATGTAATTGCGCATTTT+GGG - chr1.1:5656976-5656995 MS.gene32974:intron 30.0%
! TGTCTATTTTCAAAAGGCAA+AGG - chr1.1:5660240-5660259 MS.gene32974:intron 30.0%
! TGTGTATTTCATGGATCTTT+TGG - chr1.1:5659540-5659559 MS.gene32974:intron 30.0%
! TGTTAGAGAAATTAGTTTCG+AGG + chr1.1:5659710-5659729 None:intergenic 30.0%
! TTCTCAATGTGACTTTTTAG+TGG - chr1.1:5657592-5657611 MS.gene32974:intron 30.0%
! TTGTGTACTTGTTAACAAAG+GGG + chr1.1:5659081-5659100 None:intergenic 30.0%
!! AAGATGGTTTTTCAGAAACT+GGG - chr1.1:5656804-5656823 MS.gene32974:CDS 30.0%
!! CATTTTTCCGTTCCTTATTT+AGG + chr1.1:5656783-5656802 None:intergenic 30.0%
!! TAAGATGGTTTTTCAGAAAC+TGG - chr1.1:5656803-5656822 MS.gene32974:CDS 30.0%
!!! CATGTCTATTGTTTTAGATC+CGG + chr1.1:5659133-5659152 None:intergenic 30.0%
!!! GACATTTTAATGGATTATCC+TGG - chr1.1:5657919-5657938 MS.gene32974:intron 30.0%
AAACTGGGTTTACTCTTGAT+TGG - chr1.1:5656819-5656838 MS.gene32974:CDS 35.0%
AACCCTAATACCCAATAGAA+AGG - chr1.1:5657176-5657195 MS.gene32974:intron 35.0%
AAGGCAATAGTTAAGTGATC+AGG + chr1.1:5659157-5659176 None:intergenic 35.0%
ACCAAACACTTAGTTATGCT+AGG - chr1.1:5660305-5660324 MS.gene32974:CDS 35.0%
AGAAAGAATCACCAGCAATT+AGG + chr1.1:5657562-5657581 None:intergenic 35.0%
AGGAACGGAAAAATGTAAGA+TGG - chr1.1:5656788-5656807 MS.gene32974:CDS 35.0%
AGGAGAATGAACAAAGAGAA+AGG + chr1.1:5658244-5658263 None:intergenic 35.0%
ATAAGTGTTTGTGAGAAACG+GGG - chr1.1:5657024-5657043 MS.gene32974:intron 35.0%
ATTAGGGTAGGAAAGAAACT+AGG + chr1.1:5656550-5656569 None:intergenic 35.0%
ATTAGGTGTATCTGCAAACA+AGG + chr1.1:5657665-5657684 None:intergenic 35.0%
CACAACATTGAAATTAGGGT+AGG + chr1.1:5656562-5656581 None:intergenic 35.0%
CATAAGTGTTTGTGAGAAAC+GGG - chr1.1:5657023-5657042 MS.gene32974:intron 35.0%
CCAAACACTTAGTTATGCTA+GGG - chr1.1:5660306-5660325 MS.gene32974:CDS 35.0%
CGTCCTTAGTGTAAAAATTG+AGG - chr1.1:5658983-5659002 MS.gene32974:intron 35.0%
CTAGAAGCTAAATCCTAACT+GGG + chr1.1:5657302-5657321 None:intergenic 35.0%
CTGAGAAACTTCTGTGAATT+TGG + chr1.1:5656430-5656449 None:intergenic 35.0%
CTTGTGAAGTAAAATGGTTG+TGG - chr1.1:5656933-5656952 MS.gene32974:intron 35.0%
GAAAGAAACTAGGGTTTAGT+AGG + chr1.1:5656540-5656559 None:intergenic 35.0%
GGAGAATGAACAAAGAGAAA+GGG + chr1.1:5658243-5658262 None:intergenic 35.0%
GGCTTATCAAGTAAAAGCAA+AGG + chr1.1:5660123-5660142 None:intergenic 35.0%
GTTTCTCACAAACACTTATG+TGG + chr1.1:5657023-5657042 None:intergenic 35.0%
TAATCTCAGACTGTTGAATC+CGG - chr1.1:5659111-5659130 MS.gene32974:CDS 35.0%
TGATTCTTTGCCTATGAGAA+AGG - chr1.1:5657361-5657380 MS.gene32974:CDS 35.0%
TGCTGAATTCCATACTGAAT+TGG - chr1.1:5659390-5659409 MS.gene32974:intron 35.0%
TTAGGGTAGGAAAGAAACTA+GGG + chr1.1:5656549-5656568 None:intergenic 35.0%
TTAGGTGTATCTGCAAACAA+GGG + chr1.1:5657664-5657683 None:intergenic 35.0%
TTGACATGGATGTGCAAATA+TGG + chr1.1:5659865-5659884 None:intergenic 35.0%
TTTCTTACTACCATGGTTTG+AGG + chr1.1:5658608-5658627 None:intergenic 35.0%
! AACGGTGTTTTACTCTTGAT+TGG - chr1.1:5656687-5656706 MS.gene32974:intron 35.0%
! CGGTTCTGTCTATTTTCAAA+AGG - chr1.1:5660234-5660253 MS.gene32974:intron 35.0%
! CGTTCCTTATTTAGGTCATT+AGG + chr1.1:5656775-5656794 None:intergenic 35.0%
! GTGAATGTAATTGCGCATTT+TGG - chr1.1:5656975-5656994 MS.gene32974:intron 35.0%
! TAACCTTTCTGTCTGGTTTA+AGG - chr1.1:5658045-5658064 MS.gene32974:intron 35.0%
! TGGGTAGTTTGCATCAAAAT+TGG + chr1.1:5658376-5658395 None:intergenic 35.0%
!! AGGATTTAGCTTCTAGCTTA+TGG - chr1.1:5657306-5657325 MS.gene32974:CDS 35.0%
!! CATGCTTTGAAGAACAAAAC+AGG + chr1.1:5658012-5658031 None:intergenic 35.0%
!! CTGTTTTGTTCTTCAAAGCA+TGG - chr1.1:5658010-5658029 MS.gene32974:intron 35.0%
!! GGATTTAGCTTCTAGCTTAT+GGG - chr1.1:5657307-5657326 MS.gene32974:CDS 35.0%
!! TACGGATTAGAAAACAATGC+AGG + chr1.1:5658264-5658283 None:intergenic 35.0%
!! TTGTTTTGTTCCTCAAACCA+TGG - chr1.1:5658595-5658614 MS.gene32974:intron 35.0%
!! TTTACTTCTGAAGTTGGACT+TGG - chr1.1:5659663-5659682 MS.gene32974:intron 35.0%
AAAAGACCAGAGCAACTGAT+TGG + chr1.1:5658219-5658238 None:intergenic 40.0%
AACCTTACTACACTGATGCA+CGG - chr1.1:5659042-5659061 MS.gene32974:intron 40.0%
AACGGTTGAGTTCAATCATC+AGG + chr1.1:5659616-5659635 None:intergenic 40.0%
AATGTCGTGCAAAGAGATTC+AGG + chr1.1:5660144-5660163 None:intergenic 40.0%
ACTTAGTTATGCTAGGGAGT+TGG - chr1.1:5660312-5660331 MS.gene32974:CDS 40.0%
AGTTCAATCATCAGGTAGGT+AGG + chr1.1:5659608-5659627 None:intergenic 40.0%
CCCTAGCATAACTAAGTGTT+TGG + chr1.1:5660309-5660328 None:intergenic 40.0%
CCTATGAGAAAGGCCTTAAA+CGG - chr1.1:5657371-5657390 MS.gene32974:CDS 40.0%
CCTGAATGGGAAATCAAGAA+CGG - chr1.1:5660214-5660233 MS.gene32974:intron 40.0%
CGTTCTTGATTTCCCATTCA+GGG + chr1.1:5660216-5660235 None:intergenic 40.0%
GCTAGAAGCTAAATCCTAAC+TGG + chr1.1:5657303-5657322 None:intergenic 40.0%
GGATAGATAACCTTTCTGTC+TGG - chr1.1:5658038-5658057 MS.gene32974:intron 40.0%
GTTGAGTTCAATCATCAGGT+AGG + chr1.1:5659612-5659631 None:intergenic 40.0%
TAACTGCTTGTCAGGTGAAT+AGG - chr1.1:5658874-5658893 MS.gene32974:intron 40.0%
TAAGTGTTTGTGAGAAACGG+GGG - chr1.1:5657025-5657044 MS.gene32974:intron 40.0%
TACAGGTAAGAAAACAACGC+AGG + chr1.1:5657774-5657793 None:intergenic 40.0%
TACGGGTAAGAAAACAATGC+AGG + chr1.1:5657822-5657841 None:intergenic 40.0%
TGAGACTTCTTGTGCAGAAT+AGG - chr1.1:5659365-5659384 MS.gene32974:intron 40.0%
TGGAGCAATCCTTTATGGAA+GGG - chr1.1:5659467-5659486 MS.gene32974:intron 40.0%
TGGTTTATACTGACCCAGTT+AGG - chr1.1:5657286-5657305 MS.gene32974:CDS 40.0%
TTAAGAGGGGTCGTTAGAAA+CGG - chr1.1:5656669-5656688 MS.gene32974:intron 40.0%
TTCATTCCAATCAGTTGCTC+TGG - chr1.1:5658210-5658229 MS.gene32974:intron 40.0%
TTGGAGCAATCCTTTATGGA+AGG - chr1.1:5659466-5659485 MS.gene32974:intron 40.0%
TTTGTCATGCAGCAAGACAA+AGG + chr1.1:5658676-5658695 None:intergenic 40.0%
! AACAGGTTGAGACCAAGTAT+TGG + chr1.1:5659346-5659365 None:intergenic 40.0%
! ACAGGTTGAGACCAAGTATT+GGG + chr1.1:5659345-5659364 None:intergenic 40.0%
! ATCTTTTGGCAAAGACCATC+AGG - chr1.1:5659554-5659573 MS.gene32974:intron 40.0%
! GTCGAGAATTTTGTCCAAAC+TGG - chr1.1:5657516-5657535 MS.gene32974:intron 40.0%
! GTTAGCTCTTGAACTGCATT+AGG + chr1.1:5657682-5657701 None:intergenic 40.0%
! TAGTGGCATTTTTCACTTGC+AGG - chr1.1:5657609-5657628 MS.gene32974:intron 40.0%
! TTCGGCCAAATTTTCATTGC+AGG + chr1.1:5659787-5659806 None:intergenic 40.0%
!! ACTTGCAGGTGTTACTAACA+GGG - chr1.1:5657623-5657642 MS.gene32974:intron 40.0%
!! AGATTTTAGTGGCAGGTTGT+TGG - chr1.1:5657128-5657147 MS.gene32974:intron 40.0%
!! CACGACATTGTTTTGTTCTC+CGG - chr1.1:5660155-5660174 MS.gene32974:intron 40.0%
!! CTCTTGATTGGTTGTTTCTG+TGG - chr1.1:5656831-5656850 MS.gene32974:CDS 40.0%
!! GAAGTGTGTGTTTAGTTCTG+CGG + chr1.1:5658524-5658543 None:intergenic 40.0%
!! GATTTTAGTGGCAGGTTGTT+GGG - chr1.1:5657129-5657148 MS.gene32974:intron 40.0%
!! TGTTTGGTTGATGAGTTGCA+GGG + chr1.1:5660293-5660312 None:intergenic 40.0%
AAAGGATTGCTCCAACTCCA+CGG + chr1.1:5659461-5659480 None:intergenic 45.0%
AACGGGGGTTTACTCTTGAT+TGG - chr1.1:5657040-5657059 MS.gene32974:intron 45.0%
ACGTTCAGAGATGAGATAGC+AGG - chr1.1:5659910-5659929 MS.gene32974:intron 45.0%
ACTGTTCTATCTGGTTGTGC+TGG - chr1.1:5658162-5658181 MS.gene32974:intron 45.0%
AGGTTGTTGGGTGGCAATTT+GGG - chr1.1:5657141-5657160 MS.gene32974:intron 45.0%
ATCACAAAGAGCTCATAGCC+AGG + chr1.1:5657940-5657959 None:intergenic 45.0%
ATTGCTTTCATCCACTGCTC+TGG - chr1.1:5659414-5659433 MS.gene32974:intron 45.0%
CCACCTTAAACCAGACAGAA+AGG + chr1.1:5658051-5658070 None:intergenic 45.0%
CCGTTCTTGATTTCCCATTC+AGG + chr1.1:5660217-5660236 None:intergenic 45.0%
GATCCACTTCCAACTATGAC+AGG + chr1.1:5657990-5658009 None:intergenic 45.0%
GCAGCTTGAATTCATAGCTC+TGG - chr1.1:5657266-5657285 MS.gene32974:CDS 45.0%
GGAGCAATCCTTTATGGAAG+GGG - chr1.1:5659468-5659487 MS.gene32974:intron 45.0%
GGAGTTGGAGCAATCCTTTA+TGG - chr1.1:5659462-5659481 MS.gene32974:intron 45.0%
GTTAGAGTGTACCTTGAGCT+GGG + chr1.1:5656517-5656536 None:intergenic 45.0%
GTTCTTCAAAGCATGGCAGT+AGG - chr1.1:5658017-5658036 MS.gene32974:intron 45.0%
GTTGTATGCCCCTTCCATAA+AGG + chr1.1:5659479-5659498 None:intergenic 45.0%
TACCGTGCATCAGTGTAGTA+AGG + chr1.1:5659047-5659066 None:intergenic 45.0%
TATCTGCAAACAAGGGTGGA+AGG + chr1.1:5657657-5657676 None:intergenic 45.0%
TATTAAGCATGCCGTGGAGT+TGG - chr1.1:5659447-5659466 MS.gene32974:intron 45.0%
TCCAACTATGACAGGAGGTA+TGG + chr1.1:5657982-5658001 None:intergenic 45.0%
TCCACGGCATGCTTAATACA+AGG + chr1.1:5659445-5659464 None:intergenic 45.0%
TCCATACCTCCTGTCATAGT+TGG - chr1.1:5657978-5657997 MS.gene32974:intron 45.0%
TCCTTGTATTAAGCATGCCG+TGG - chr1.1:5659441-5659460 MS.gene32974:intron 45.0%
TCTGCACAAGAAGTCTCAAC+AGG + chr1.1:5659363-5659382 None:intergenic 45.0%
! CCGTTTAAGGCCTTTCTCAT+AGG + chr1.1:5657374-5657393 None:intergenic 45.0%
! CCTTTCTGTCTGGTTTAAGG+TGG - chr1.1:5658048-5658067 MS.gene32974:intron 45.0%
! TTCCAAGAGTTCCTGGTCTT+TGG + chr1.1:5659998-5660017 None:intergenic 45.0%
!! AGGGAGGGTATTTCTTTGCA+AGG + chr1.1:5660274-5660293 None:intergenic 45.0%
!! ATTTCAGGAGCACCCTGAAT+GGG - chr1.1:5660201-5660220 MS.gene32974:intron 45.0%
!! CACTTGCAGGTGTTACTAAC+AGG - chr1.1:5657622-5657641 MS.gene32974:intron 45.0%
!! GTGTTTGGTTGATGAGTTGC+AGG + chr1.1:5660294-5660313 None:intergenic 45.0%
!! TTTATTAAAAAAAAAAATAC+GGG + chr1.1:5657839-5657858 None:intergenic 5.0%
ACAAGGATTCTCCAGAGCAG+TGG + chr1.1:5659428-5659447 None:intergenic 50.0%
AGTGTACCTTGAGCTGGGAA+AGG + chr1.1:5656512-5656531 None:intergenic 50.0%
CACCAAAGACCAGGAACTCT+TGG - chr1.1:5659993-5660012 MS.gene32974:intron 50.0%
CAGGTTGTTGGGTGGCAATT+TGG - chr1.1:5657140-5657159 MS.gene32974:intron 50.0%
CCACTTCCAACTATGACAGG+AGG + chr1.1:5657987-5658006 None:intergenic 50.0%
CCTCCTGTCATAGTTGGAAG+TGG - chr1.1:5657984-5658003 MS.gene32974:intron 50.0%
CTGGGAAAGGAGATTCGAAG+CGG + chr1.1:5656499-5656518 None:intergenic 50.0%
GAATCTCCTTTCCCAGCTCA+AGG - chr1.1:5656503-5656522 MS.gene32974:CDS 50.0%
GATAGCAGGATGCAGTGAGA+AGG - chr1.1:5659924-5659943 MS.gene32974:intron 50.0%
GGTGTATCTGCAAACAAGGG+TGG + chr1.1:5657661-5657680 None:intergenic 50.0%
GGTTAGAGTGTACCTTGAGC+TGG + chr1.1:5656518-5656537 None:intergenic 50.0%
GTTATGCTAGGGAGTTGGAG+AGG - chr1.1:5660317-5660336 MS.gene32974:CDS 50.0%
TCTGTCGAGCACTCAAGACA+CGG + chr1.1:5659501-5659520 None:intergenic 50.0%
TGGGTACTCCTGAGGAGATT+GGG + chr1.1:5659326-5659345 None:intergenic 50.0%
TTGGGTACTCCTGAGGAGAT+TGG + chr1.1:5659327-5659346 None:intergenic 50.0%
TTTAGTGGCAGGTTGTTGGG+TGG - chr1.1:5657132-5657151 MS.gene32974:intron 50.0%
! GTTGCTGTTCACCAAAGACC+AGG - chr1.1:5659984-5660003 MS.gene32974:intron 50.0%
!! CCAAGTATTGGGTACTCCTG+AGG + chr1.1:5659334-5659353 None:intergenic 50.0%
!! CCTCAGGAGTACCCAATACT+TGG - chr1.1:5659331-5659350 MS.gene32974:intron 50.0%
!! GATTTCAGGAGCACCCTGAA+TGG - chr1.1:5660200-5660219 MS.gene32974:intron 50.0%
!! TGGTTGATGAGTTGCAGGGA+GGG + chr1.1:5660289-5660308 None:intergenic 50.0%
!! TTGGTTGATGAGTTGCAGGG+AGG + chr1.1:5660290-5660309 None:intergenic 50.0%
GGGTACTCCTGAGGAGATTG+GGG + chr1.1:5659325-5659344 None:intergenic 55.0%
TCGTCTTCCCCAATCTCCTC+AGG - chr1.1:5659315-5659334 MS.gene32974:intron 55.0%
! ACAGAGGTGTGCGAGCTTCA+AGG + chr1.1:5659828-5659847 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 5656420 5660348 5656420 ID=MS.gene32974
chr1.1 mRNA 5656420 5660348 5656420 ID=MS.gene32974.t1;Parent=MS.gene32974
chr1.1 exon 5660244 5660348 5660244 ID=MS.gene32974.t1.exon1;Parent=MS.gene32974.t1
chr1.1 CDS 5660244 5660348 5660244 ID=cds.MS.gene32974.t1;Parent=MS.gene32974.t1
chr1.1 exon 5659059 5659137 5659059 ID=MS.gene32974.t1.exon2;Parent=MS.gene32974.t1
chr1.1 CDS 5659059 5659137 5659059 ID=cds.MS.gene32974.t1;Parent=MS.gene32974.t1
chr1.1 exon 5657705 5657807 5657705 ID=MS.gene32974.t1.exon3;Parent=MS.gene32974.t1
chr1.1 CDS 5657705 5657807 5657705 ID=cds.MS.gene32974.t1;Parent=MS.gene32974.t1
chr1.1 exon 5657193 5657456 5657193 ID=MS.gene32974.t1.exon4;Parent=MS.gene32974.t1
chr1.1 CDS 5657193 5657456 5657193 ID=cds.MS.gene32974.t1;Parent=MS.gene32974.t1
chr1.1 exon 5656739 5656850 5656739 ID=MS.gene32974.t1.exon5;Parent=MS.gene32974.t1
chr1.1 CDS 5656739 5656850 5656739 ID=cds.MS.gene32974.t1;Parent=MS.gene32974.t1
chr1.1 exon 5656420 5656560 5656420 ID=MS.gene32974.t1.exon6;Parent=MS.gene32974.t1
chr1.1 CDS 5656420 5656560 5656420 ID=cds.MS.gene32974.t1;Parent=MS.gene32974.t1
Gene Sequence

>MS.gene32974

ATGGATCCCAAATTCACAGAAGTTTCTCAGTTGTTCGATCGATTCAAAGCTGCTTTTCTCAGAAACGACTTCGATTCCGCTTCGAATCTCCTTTCCCAGCTCAAGGTACACTCTAACCCTACTAAACCCTAGTTTCTTTCCTACCCTAATTTCAATGTTGTGTGTTGCTTTAGTTTTAGATCGTTGTATTAAATTAATTGTGAAATTCATATGTAATTTTGATTTAATTAGTTAATTTGTATAAAAAAATTAAGAGGGGTCGTTAGAAACGGTGTTTTACTCTTGATTGGTTGTTTGTGTTGATTTTGTGCTGTATAGTGTGTATGTTAGATTTGAATTTTAAAGTATATAACCTAATGACCTAAATAAGGAACGGAAAAATGTAAGATGGTTTTTCAGAAACTGGGTTTACTCTTGATTGGTTGTTTCTGTGGTAGATTTGAATTGTGAAGCATATAACCTATAAAAGAAACATATTTTAGAGAGGAACTGTTAGAAAAACTTGTAGATATTCTTGTGAAGTAAAATGGTTGTGGGTTGATTCAGTGCGAGATTGTGAATGTAATTGCGCATTTTGGGTCAGATGTATTACTGTCACACCCACATAAGTGTTTGTGAGAAACGGGGGTTTACTCTTGATTGGTACTGATTGAGTTGAATTTTTCGTGTGCTAGATTTGAAATTTAAAATATTTAGCCTAAAAATAAAAGATTTTAGTGGCAGGTTGTTGGGTGGCAATTTGGGACTTTCTCATCAAACCCTAATACCCAATAGAAAGGAAACTATTGCTTTGAAAGCATTGTGTCTTGATACTTGTTAGTTATTGGGCTTATAGGATGACTTCGAGCAGCTTGAATTCATAGCTCTGGTTTATACTGACCCAGTTAGGATTTAGCTTCTAGCTTATGGGATTTTTTTTGTTTGAATAAGATTATGAATTGTGATTCTTTGCCTATGAGAAAGGCCTTAAACGGTTAGACAAAGTTTTTGAAAAAAATTGAGATGTAGAAGTTAATTACAACTTATACAAGAAACTGTTGTTTAATAAACTCAGTTATTGTAGGGTTCCTTATCATTTAGTTTTCCATAATTATTTGTCGAGAATTTTGTCCAAACTGGCTATAGTCTTTAAAATAGCACCTAATTGCTGGTGATTCTTTCTGTTTTTTCAATTCTCAATGTGACTTTTTAGTGGCATTTTTCACTTGCAGGTGTTACTAACAGGGTTCAGAAGCCTTCCACCCTTGTTTGCAGATACACCTAATGCAGTTCAAGAGCTAACTATTGCAAGTAATACTCTTCATTCCATTCAGTTTCTATGGTGTTTTTTTACTTTTTCTTTGTTCATTCTCCTGCGTTGTTTTCTTACCTGTATTTTTACTTTTTCTTTGTTCATTCTCCTGCATTGTTTTCTTACCCGTATTTTTTTTTTTAATAAAAGAAGATCCACCTTATCAAATATCATATGAGTGAATCTTAAATTAGGTTGTATTGTAAATGACATTTTAATGGATTATCCTGGCTATGAGCTCTTTGTGATATAAATTTGAATTATTCTTCCATACCTCCTGTCATAGTTGGAAGTGGATCCTGTTTTGTTCTTCAAAGCATGGCAGTAGGATAGATAACCTTTCTGTCTGGTTTAAGGTGGAATTTCAAGTTAGCGTACTGCATGACAAAACTGTATATTAGAAGAGACAAGAAAATAGTTTTTATTTCTAAAATTGGAAGTAAGATAGATAACTGTTCTATCTGGTTGTGCTGGAATTGCAAATTAGCAAGTAATATTCTTCATTCCAATCAGTTGCTCTGGTCTTTTTCCCTTTCTCTTTGTTCATTCTCCTGCATTGTTTTCTAATCCGTATTTTGTGTTATTAGTAATAAAAGAAGATAAACTTCTTATATAGTTTTAAATATATTAATAAAACCTATTTTAGTAATATACATATAAAACCAATTTTGATGCAAACTACCCAAACATCATAAATTAACACAATCACTTTTATGTTAATGTATCCAAACATAAATCAATTATGTTAAACTCAATTTTAATCAAAATCAATTATCTCAAAATCAATTATTTCATAATCAATTCTGGCAACCGCAGAACTAAACACACACTTCATCAAATATCATGCGAGTGAATCTTAAATTAAATTAGGTTGGAAGAGGATCTTGTTTTGTTCCTCAAACCATGGTAGTAAGAAAGATAGATAAATTTTTCTATCTGGTTGTTGTAGAACTGCAAGTTAACCTTTGTCTTGCTGCATGACAAATACTGTATTTTAGAGGAGACAAAAATACAAGAGTTTTTATACTAAAATTGGAAACTGTTTGATGCTTCAAGTTCAGTGAAATAAAATGATGAGCCATTTTCCATATCAATTATACAAAATAAAATACTGAAACTTTTCTCCCATGACTCGATCATATTAATTAGAATGAATAAGATAATAACTGCTTGTCAGGTGAATAGGAAAAAGTTGCTAGCTATTAGTTCTTTGTCTGAAATGTTTTTTTCTTCTTGTTGAAAATTTTCAGGGGATATATACGAACATGCCGTCGTCCTTAGTGTAAAAATTGAGGATCAAGATGCCTTTGAAAGAGATTTCTTTCAATTGAAACCTTACTACACTGATGCACGGTAAATGATTCTTTCCCCTTTGTTAACAAGTACACAATATATGTTTCTAATCTCAGACTGTTGAATCCGGATCTAAAACAATAGACATGTCCTGATCACTTAACTATTGCCTTATAGTATATTACCTTGTAGTATATACCAAGAAACGTGAAAGTATTATCTATAACAGACTGTGATTCTATATCCAACTCATTATTTGCTTTCTTGTTGAGCTATTGTAATTTATGGTGACTTTTCATGTGTTGCAGTAATCGTCTTCCCCAATCTCCTCAGGAGTACCCAATACTTGGTCTCAACCTGTTGAGACTTCTTGTGCAGAATAGGATTGCTGAATTCCATACTGAATTGGAATTGCTTTCATCCACTGCTCTGGAGAATCCTTGTATTAAGCATGCCGTGGAGTTGGAGCAATCCTTTATGGAAGGGGCATACAACCGTGTCTTGAGTGCTCGACAGACAGTACCACATGAAACATATGTGTATTTCATGGATCTTTTGGCAAAGACCATCAGGTAATAATTGTTGTCATTATTTCTTATAGCCTACCTACCTGATGATTGAACTCAACCGTTAAATTGGAACTCCATCTATTTATTTTTTTTACTTCTGAAGTTGGACTTGGTTACTTTGTTTTTATTGTTCTCCTCGAAACTAATTTCTCTAACATAATTACTATAAACTTAAAGATATCTGTAGTATTGAAGTCTATTTTTCGTAGTTCCTGCAATGAAAATTTGGCCGAAGCACAACCATTATTATAACCTTGAAGCTCGCACACCTCTGTTGTATATTGTTTTTCCATATTTGCACATCCATGTCAATTTGCTAACTAGTTTGTATCTTTAAACGTTCAGAGATGAGATAGCAGGATGCAGTGAGAAGGCATATGACTATCTTTCAATTAATGATGCTAAGCAGATGTTGCTGTTCACCAAAGACCAGGAACTCTTGGAATATATTAAGGAGGTGAGAAATGTCACTGTACATTGATGTTGTATAGTAAATATTAATTTCTGCTGTGAATGCGTAATTTTATCTTCTACGTTTCATGTTGCTCCTTTGCTTTTACTTGATAAGCCTGAATCTCTTTGCACGACATTGTTTTGTTCTCCGGTTCTATAATTAGTATATCTACTGATTTCAGGAGCACCCTGAATGGGAAATCAAGAACGGTTCTGTCTATTTTCAAAAGGCAAAGGATTCTGCACCTTGCAAAGAAATACCCTCCCTGCAACTCATCAACCAAACACTTAGTTATGCTAGGGAGTTGGAGAGGATTGTCTGA
Protein sequence

>MS.gene32974.t1

MDPKFTEVSQLFDRFKAAFLRNDFDSASNLLSQLKVLLTGFRSLPPLFADTPNAVQELTIARDIYEHAVVLSVKIEDQDAFERDFFQLKPYYTDARNRLPQSPQEYPILGLNLLRLLVQNRIAEFHTELELLSSTALENPCIKHAVELEQSFMEGAYNRVLSARQTVPHETYVYFMDLLAKTIRDEIAGCSEKAYDYLSINDAKQMLLFTKDQELLEYIKEEHPEWEIKNGSVYFQKAKDSAPCKEIPSLQLINQTLSYARELERIV