AlfalfaGEDB | MS.gene033232

M. sativa cultivar XinJiangDaYe / MS.gene033232

KEGG: K10881
GO: GO:0005575 GO:0006405 GO:0006406 GO:0006810 GO:0006913 GO:0008150 GO:0008541 GO:0009987 GO:0015931 GO:0016043 GO:0022607 GO:0032991 GO:0034622 GO:0043248 GO:0043933 GO:0046907 GO:0050657 GO:0050658 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0065003 GO:0071702 GO:0071705 GO:0071840
PFAM: PF05160.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene033232.t1	GAU19652.1	91.2	34	3	0	1	34	1	34	3.40E-07	63.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene033232.t1	Q9FL96	77.8	36	7	1	1	35	1	36	5.5e-06	51.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene033232.t1	A0A2Z6LNG4	91.2	34	3	0	1	34	1	34	2.5e-07	63.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene033232	MS.gene89672	PPI
MS.gene033232	MS.gene69373	PPI
MS.gene033232	MS.gene57218	PPI
MS.gene67099	MS.gene033232	PPI
MS.gene033232	MS.gene024828	PPI
MS.gene033232	MS.gene70283	PPI
MS.gene033232	MS.gene70282	PPI
MS.gene033232	MS.gene030685	PPI
MS.gene28454	MS.gene033232	PPI
MS.gene24542	MS.gene033232	PPI
MS.gene09974	MS.gene033232	PPI
MS.gene033232	MS.gene21169	PPI
MS.gene48400	MS.gene033232	PPI
MS.gene033232	MS.gene24375	PPI
MS.gene072221	MS.gene033232	PPI
MS.gene033232	MS.gene21801	PPI
MS.gene033232	MS.gene64789	PPI
MS.gene033232	MS.gene48279	PPI
MS.gene033232	MS.gene36633	PPI
MS.gene033232	MS.gene014393	PPI
MS.gene69461	MS.gene033232	PPI
MS.gene033232	MS.gene01697	PPI
MS.gene064694	MS.gene033232	PPI
MS.gene017622	MS.gene033232	PPI
MS.gene013607	MS.gene033232	PPI
MS.gene033232	MS.gene057145	PPI
MS.gene002306	MS.gene033232	PPI
MS.gene68079	MS.gene033232	PPI
MS.gene033232	MS.gene36746	PPI
MS.gene033232	MS.gene030686	PPI
MS.gene02265	MS.gene033232	PPI
MS.gene033232	MS.gene058061	PPI
MS.gene014485	MS.gene033232	PPI
MS.gene033232	MS.gene012127	PPI
MS.gene06729	MS.gene033232	PPI
MS.gene033232	MS.gene58136	PPI
MS.gene062770	MS.gene033232	PPI
MS.gene033232	MS.gene033335	PPI
MS.gene58134	MS.gene033232	PPI
MS.gene033232	MS.gene42312	PPI
MS.gene033232	MS.gene59485	PPI
MS.gene033232	MS.gene002648	PPI
MS.gene025808	MS.gene033232	PPI
MS.gene072220	MS.gene033232	PPI
MS.gene40486	MS.gene033232	PPI
MS.gene06946	MS.gene033232	PPI
MS.gene033232	MS.gene48281	PPI
MS.gene28183	MS.gene033232	PPI
MS.gene033232	MS.gene057144	PPI
MS.gene033232	MS.gene064786	PPI
MS.gene011376	MS.gene033232	PPI
MS.gene033232	MS.gene004328	PPI
MS.gene32974	MS.gene033232	PPI
MS.gene044013	MS.gene033232	PPI
MS.gene44230	MS.gene033232	PPI
MS.gene033232	MS.gene52815	PPI
MS.gene011065	MS.gene033232	PPI
MS.gene051630	MS.gene033232	PPI
MS.gene67460	MS.gene033232	PPI
MS.gene01284	MS.gene033232	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 17 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGACGTTCTCGGAAGGTTT+TGG	0.276388	8.4:-20599550	None:intergenic
CTCAGCAATGGGAAGATGAT+TGG	0.389575	8.4:+20599805	MS.gene033232:CDS
TCAGCTAAAGAGAGAGTTAG+AGG	0.407568	8.4:+20599860	MS.gene033232:CDS
GAGATCAACCTTGACGTTCT+CGG	0.418361	8.4:-20599560	None:intergenic
AGACAAGGAGGAAGGGAGAG+AGG	0.431303	8.4:+20599779	MS.gene033232:CDS
TGAAGACGACGACGAGTTCG+AGG	0.463513	8.4:+20599585	MS.gene033232:CDS
TCAGCAATGGGAAGATGATT+GGG	0.477084	8.4:+20599806	MS.gene033232:CDS
GGAGAGAGGTAGCTCAGCAA+TGG	0.520450	8.4:+20599793	MS.gene033232:CDS
GGTTGGGAAGACAAGGAGGA+AGG	0.522923	8.4:+20599771	MS.gene033232:intron
GAGAGAGGTAGCTCAGCAAT+GGG	0.525728	8.4:+20599794	MS.gene033232:CDS
GTTGGGAAGACAAGGAGGAA+GGG	0.529450	8.4:+20599772	MS.gene033232:CDS
TCAACCTTGACGTTCTCGGA+AGG	0.583809	8.4:-20599556	None:intergenic
ACTTGCAGGTTGGGAAGACA+AGG	0.596138	8.4:+20599764	MS.gene033232:intron
CAGCTAAAGAGAGAGTTAGA+GGG	0.638489	8.4:+20599861	MS.gene033232:CDS
GAGGAGTTTGAAATCAACGA+AGG	0.657612	8.4:+20599604	MS.gene033232:CDS
TGCAGGTTGGGAAGACAAGG+AGG	0.659645	8.4:+20599767	MS.gene033232:intron
AAAACCTTCCGAGAACGTCA+AGG	0.660047	8.4:+20599552	MS.gene033232:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TACTAACATGAATTCAAATC+TGG	-	chr8.4:20599711-20599730	None:intergenic	25.0%
	AAATTGATTGCAACAACGAT+TGG	-	chr8.4:20599668-20599687	None:intergenic	30.0%
	AACAACGATTGGAATGAAGA+GGG	-	chr8.4:20599657-20599676	None:intergenic	35.0%
!	ATCTTATGTAACTTGCAGGT+TGG	+	chr8.4:20599754-20599773	MS.gene033232:intron	35.0%
!	TCTTATGTAACTTGCAGGTT+GGG	+	chr8.4:20599755-20599774	MS.gene033232:intron	35.0%
!	TGCAATCTTATGTAACTTGC+AGG	+	chr8.4:20599750-20599769	MS.gene033232:intron	35.0%
	AATGAAGAGGGGTTAAAGGT+TGG	-	chr8.4:20599645-20599664	None:intergenic	40.0%
	ACAACGATTGGAATGAAGAG+GGG	-	chr8.4:20599656-20599675	None:intergenic	40.0%
	CAACAACGATTGGAATGAAG+AGG	-	chr8.4:20599658-20599677	None:intergenic	40.0%
	CAGCTAAAGAGAGAGTTAGA+GGG	+	chr8.4:20599861-20599880	MS.gene033232:CDS	40.0%
	TCAGCAATGGGAAGATGATT+GGG	+	chr8.4:20599806-20599825	MS.gene033232:CDS	40.0%
	TCAGCTAAAGAGAGAGTTAG+AGG	+	chr8.4:20599860-20599879	MS.gene033232:CDS	40.0%
	TTGGAATGAAGAGGGGTTAA+AGG	-	chr8.4:20599649-20599668	None:intergenic	40.0%
!	GAGGAGTTTGAAATCAACGA+AGG	+	chr8.4:20599604-20599623	MS.gene033232:CDS	40.0%
	AAAACCTTCCGAGAACGTCA+AGG	+	chr8.4:20599552-20599571	MS.gene033232:CDS	45.0%
	CTCAGCAATGGGAAGATGAT+TGG	+	chr8.4:20599805-20599824	MS.gene033232:CDS	45.0%
	GAGATCAACCTTGACGTTCT+CGG	-	chr8.4:20599563-20599582	None:intergenic	45.0%
!	TTGACGTTCTCGGAAGGTTT+TGG	-	chr8.4:20599553-20599572	None:intergenic	45.0%
	GAGAGAGGTAGCTCAGCAAT+GGG	+	chr8.4:20599794-20599813	MS.gene033232:CDS	50.0%
	GTTGGGAAGACAAGGAGGAA+GGG	+	chr8.4:20599772-20599791	MS.gene033232:CDS	50.0%
	TCAACCTTGACGTTCTCGGA+AGG	-	chr8.4:20599559-20599578	None:intergenic	50.0%
!	ACTTGCAGGTTGGGAAGACA+AGG	+	chr8.4:20599764-20599783	MS.gene033232:intron	50.0%
!!!	GTTCTCGGAAGGTTTTGGCT+CGG	-	chr8.4:20599548-20599567	None:intergenic	50.0%
	AGACAAGGAGGAAGGGAGAG+AGG	+	chr8.4:20599779-20599798	MS.gene033232:CDS	55.0%
	GGAGAGAGGTAGCTCAGCAA+TGG	+	chr8.4:20599793-20599812	MS.gene033232:CDS	55.0%
	GGTTGGGAAGACAAGGAGGA+AGG	+	chr8.4:20599771-20599790	MS.gene033232:intron	55.0%
	TGAAGACGACGACGAGTTCG+AGG	+	chr8.4:20599585-20599604	MS.gene033232:CDS	55.0%
!	TGCAGGTTGGGAAGACAAGG+AGG	+	chr8.4:20599767-20599786	MS.gene033232:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	20599538	20599902	20599538	ID=MS.gene033232
chr8.4	mRNA	20599538	20599902	20599538	ID=MS.gene033232.t1;Parent=MS.gene033232
chr8.4	exon	20599538	20599625	20599538	ID=MS.gene033232.t1.exon1;Parent=MS.gene033232.t1
chr8.4	CDS	20599538	20599625	20599538	ID=cds.MS.gene033232.t1;Parent=MS.gene033232.t1
chr8.4	exon	20599772	20599902	20599772	ID=MS.gene033232.t1.exon2;Parent=MS.gene033232.t1
chr8.4	CDS	20599772	20599902	20599772	ID=cds.MS.gene033232.t1;Parent=MS.gene033232.t1

Gene Sequence	>MS.gene033232 ATGGCAACCGAGCCAAAACCTTCCGAGAACGTCAAGGTTGATCTCTTTGAAGACGACGACGAGTTCGAGGAGTTTGAAATCAACGAAGGTAACTTCAAATCGTTCCAACCTTTAACCCCTCTTCATTCCAATCGTTGTTGCAATCAATTTCAGCTTCATTCAATTTCGATCCAGATTTGAATTCATGTTAGTAGTTTCTTATTGTTTATTTTTGCAATCTTATGTAACTTGCAGGTTGGGAAGACAAGGAGGAAGGGAGAGAGGTAGCTCAGCAATGGGAAGATGATTGGGATGATGATGATGTTAGTGATGATTTCTCTCTTCAGCTAAAGAGAGAGTTAGAGGGCAATGCTGAGAAGAAATAA
Protein sequence	>MS.gene033232.t1 MATEPKPSENVKVDLFEDDDEFEEFEINEGWEDKEEGREVAQQWEDDWDDDDVSDDFSLQLKRELEGNAEKK