Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34412.t1 | XP_013469722.1 | 85.9 | 262 | 10 | 2 | 1 | 235 | 1 | 262 | 1.70E-95 | 359 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34412.t1 | A0A072W0C2 | 85.9 | 262 | 10 | 2 | 1 | 235 | 1 | 262 | 1.2e-95 | 359.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene34412 | -0.858448 | 8.18E-63 | -1.69E-46 |
| MS.gene049181 | MS.gene34412 | 0.814838 | 1.26E-51 | -1.69E-46 |
| MS.gene049217 | MS.gene34412 | -0.858381 | 8.57E-63 | -1.69E-46 |
| MS.gene049222 | MS.gene34412 | -0.845972 | 2.91E-59 | -1.69E-46 |
| MS.gene049232 | MS.gene34412 | -0.821873 | 3.20E-53 | -1.69E-46 |
| MS.gene049281 | MS.gene34412 | 0.861588 | 9.23E-64 | -1.69E-46 |
| MS.gene049285 | MS.gene34412 | -0.869283 | 3.48E-66 | -1.69E-46 |
| MS.gene049312 | MS.gene34412 | 0.832176 | 1.10E-55 | -1.69E-46 |
| MS.gene049313 | MS.gene34412 | 0.844473 | 7.40E-59 | -1.69E-46 |
| MS.gene049320 | MS.gene34412 | -0.878305 | 3.13E-69 | -1.69E-46 |
| MS.gene049483 | MS.gene34412 | 0.838028 | 3.67E-57 | -1.69E-46 |
| MS.gene049609 | MS.gene34412 | -0.827959 | 1.17E-54 | -1.69E-46 |
| MS.gene049627 | MS.gene34412 | -0.853022 | 3.14E-61 | -1.69E-46 |
| MS.gene049633 | MS.gene34412 | -0.806714 | 7.19E-50 | -1.69E-46 |
| MS.gene05010 | MS.gene34412 | -0.857593 | 1.47E-62 | -1.69E-46 |
| MS.gene050129 | MS.gene34412 | 0.859683 | 3.49E-63 | -1.69E-46 |
| MS.gene050163 | MS.gene34412 | -0.862166 | 6.14E-64 | -1.69E-46 |
| MS.gene050165 | MS.gene34412 | -0.854837 | 9.43E-62 | -1.69E-46 |
| MS.gene050170 | MS.gene34412 | -0.810729 | 9.97E-51 | -1.69E-46 |
| MS.gene050216 | MS.gene34412 | -0.801604 | 8.33E-49 | -1.69E-46 |
| MS.gene050333 | MS.gene34412 | -0.829126 | 6.13E-55 | -1.69E-46 |
| MS.gene050353 | MS.gene34412 | 0.814791 | 1.29E-51 | -1.69E-46 |
| MS.gene050363 | MS.gene34412 | 0.854606 | 1.10E-61 | -1.69E-46 |
| MS.gene050381 | MS.gene34412 | 0.864524 | 1.14E-64 | -1.69E-46 |
| MS.gene050493 | MS.gene34412 | 0.822979 | 1.77E-53 | -1.69E-46 |
| MS.gene050496 | MS.gene34412 | -0.833506 | 5.13E-56 | -1.69E-46 |
| MS.gene050504 | MS.gene34412 | -0.891197 | 4.95E-74 | -1.69E-46 |
| MS.gene050518 | MS.gene34412 | -0.841338 | 5.05E-58 | -1.69E-46 |
| MS.gene050577 | MS.gene34412 | -0.84243 | 2.60E-58 | -1.69E-46 |
| MS.gene050612 | MS.gene34412 | -0.873826 | 1.09E-67 | -1.69E-46 |
| MS.gene050675 | MS.gene34412 | -0.850866 | 1.29E-60 | -1.69E-46 |
| MS.gene050699 | MS.gene34412 | -0.866642 | 2.46E-65 | -1.69E-46 |
| MS.gene050747 | MS.gene34412 | -0.80788 | 4.07E-50 | -1.69E-46 |
| MS.gene050772 | MS.gene34412 | -0.845472 | 3.98E-59 | -1.69E-46 |
| MS.gene050868 | MS.gene34412 | -0.828492 | 8.72E-55 | -1.69E-46 |
| MS.gene050871 | MS.gene34412 | -0.830161 | 3.43E-55 | -1.69E-46 |
| MS.gene050883 | MS.gene34412 | 0.863782 | 1.95E-64 | -1.69E-46 |
| MS.gene050950 | MS.gene34412 | -0.802124 | 6.51E-49 | -1.69E-46 |
| MS.gene050986 | MS.gene34412 | -0.876524 | 1.30E-68 | -1.69E-46 |
| MS.gene05100 | MS.gene34412 | -0.834209 | 3.42E-56 | -1.69E-46 |
| MS.gene051044 | MS.gene34412 | 0.800806 | 1.21E-48 | -1.69E-46 |
| MS.gene051127 | MS.gene34412 | 0.856952 | 2.27E-62 | -1.69E-46 |
| MS.gene051364 | MS.gene34412 | 0.816174 | 6.34E-52 | -1.69E-46 |
| MS.gene051366 | MS.gene34412 | 0.801474 | 8.85E-49 | -1.69E-46 |
| MS.gene05141 | MS.gene34412 | 0.84173 | 3.98E-58 | -1.69E-46 |
| MS.gene051551 | MS.gene34412 | -0.836435 | 9.37E-57 | -1.69E-46 |
| MS.gene051564 | MS.gene34412 | -0.84582 | 3.20E-59 | -1.69E-46 |
| MS.gene051566 | MS.gene34412 | -0.822637 | 2.13E-53 | -1.69E-46 |
| MS.gene051694 | MS.gene34412 | -0.831098 | 2.02E-55 | -1.69E-46 |
| MS.gene051810 | MS.gene34412 | -0.8413 | 5.17E-58 | -1.69E-46 |
| MS.gene051858 | MS.gene34412 | -0.824203 | 9.17E-54 | -1.69E-46 |
| MS.gene051946 | MS.gene34412 | 0.864639 | 1.05E-64 | -1.69E-46 |
| MS.gene052164 | MS.gene34412 | -0.872542 | 2.94E-67 | -1.69E-46 |
| MS.gene052482 | MS.gene34412 | -0.84863 | 5.42E-60 | -1.69E-46 |
| MS.gene052486 | MS.gene34412 | -0.855426 | 6.36E-62 | -1.69E-46 |
| MS.gene052503 | MS.gene34412 | -0.820243 | 7.60E-53 | -1.69E-46 |
| MS.gene052615 | MS.gene34412 | -0.813661 | 2.29E-51 | -1.69E-46 |
| MS.gene052670 | MS.gene34412 | -0.817212 | 3.71E-52 | -1.69E-46 |
| MS.gene052823 | MS.gene34412 | -0.823264 | 1.52E-53 | -1.69E-46 |
| MS.gene052825 | MS.gene34412 | -0.814952 | 1.19E-51 | -1.69E-46 |
| MS.gene052936 | MS.gene34412 | 0.825753 | 3.95E-54 | -1.69E-46 |
| MS.gene052937 | MS.gene34412 | 0.83196 | 1.24E-55 | -1.69E-46 |
| MS.gene052966 | MS.gene34412 | -0.817599 | 3.03E-52 | -1.69E-46 |
| MS.gene053203 | MS.gene34412 | -0.853537 | 2.24E-61 | -1.69E-46 |
| MS.gene053207 | MS.gene34412 | -0.807421 | 5.10E-50 | -1.69E-46 |
| MS.gene053213 | MS.gene34412 | -0.861783 | 8.05E-64 | -1.69E-46 |
| MS.gene053286 | MS.gene34412 | -0.864049 | 1.61E-64 | -1.69E-46 |
| MS.gene053420 | MS.gene34412 | 0.866111 | 3.62E-65 | -1.69E-46 |
| MS.gene053436 | MS.gene34412 | -0.822466 | 2.33E-53 | -1.69E-46 |
| MS.gene053549 | MS.gene34412 | 0.874307 | 7.48E-68 | -1.69E-46 |
| MS.gene053561 | MS.gene34412 | 0.848029 | 7.95E-60 | -1.69E-46 |
| MS.gene053574 | MS.gene34412 | -0.837578 | 4.79E-57 | -1.69E-46 |
| MS.gene053632 | MS.gene34412 | -0.821777 | 3.37E-53 | -1.69E-46 |
| MS.gene053824 | MS.gene34412 | -0.810213 | 1.29E-50 | -1.69E-46 |
| MS.gene053826 | MS.gene34412 | 0.813949 | 1.98E-51 | -1.69E-46 |
| MS.gene054595 | MS.gene34412 | -0.829885 | 4.01E-55 | -1.69E-46 |
| MS.gene054600 | MS.gene34412 | -0.829913 | 3.95E-55 | -1.69E-46 |
| MS.gene054616 | MS.gene34412 | 0.849966 | 2.30E-60 | -1.69E-46 |
| MS.gene054642 | MS.gene34412 | -0.859568 | 3.78E-63 | -1.69E-46 |
| MS.gene054706 | MS.gene34412 | 0.814627 | 1.40E-51 | -1.69E-46 |
| MS.gene054708 | MS.gene34412 | 0.847887 | 8.70E-60 | -1.69E-46 |
| MS.gene05470 | MS.gene34412 | -0.849814 | 2.54E-60 | -1.69E-46 |
| MS.gene054927 | MS.gene34412 | -0.825685 | 4.10E-54 | -1.69E-46 |
| MS.gene054954 | MS.gene34412 | -0.875612 | 2.69E-68 | -1.69E-46 |
| MS.gene054959 | MS.gene34412 | 0.855669 | 5.40E-62 | -1.69E-46 |
| MS.gene055154 | MS.gene34412 | 0.855091 | 7.96E-62 | -1.69E-46 |
| MS.gene055362 | MS.gene34412 | -0.893477 | 6.06E-75 | -1.69E-46 |
| MS.gene055502 | MS.gene34412 | -0.86778 | 1.06E-65 | -1.69E-46 |
| MS.gene055577 | MS.gene34412 | -0.862695 | 4.22E-64 | -1.69E-46 |
| MS.gene055722 | MS.gene34412 | -0.81688 | 4.40E-52 | -1.69E-46 |
| MS.gene055751 | MS.gene34412 | -0.804538 | 2.06E-49 | -1.69E-46 |
| MS.gene055776 | MS.gene34412 | -0.810186 | 1.31E-50 | -1.69E-46 |
| MS.gene05589 | MS.gene34412 | -0.839958 | 1.16E-57 | -1.69E-46 |
| MS.gene055941 | MS.gene34412 | -0.811052 | 8.49E-51 | -1.69E-46 |
| MS.gene056046 | MS.gene34412 | -0.809099 | 2.24E-50 | -1.69E-46 |
| MS.gene056083 | MS.gene34412 | -0.823859 | 1.10E-53 | -1.69E-46 |
| MS.gene056310 | MS.gene34412 | 0.820563 | 6.42E-53 | -1.69E-46 |
| MS.gene056311 | MS.gene34412 | 0.859004 | 5.58E-63 | -1.69E-46 |
| MS.gene056313 | MS.gene34412 | 0.801434 | 9.02E-49 | -1.69E-46 |
| MS.gene056374 | MS.gene34412 | -0.84045 | 8.63E-58 | -1.69E-46 |
| MS.gene056408 | MS.gene34412 | 0.846786 | 1.74E-59 | -1.69E-46 |
| MS.gene056409 | MS.gene34412 | -0.837415 | 5.27E-57 | -1.69E-46 |
| MS.gene056504 | MS.gene34412 | -0.863583 | 2.25E-64 | -1.69E-46 |
| MS.gene056510 | MS.gene34412 | -0.838478 | 2.81E-57 | -1.69E-46 |
| MS.gene056550 | MS.gene34412 | 0.87833 | 3.06E-69 | -1.69E-46 |
| MS.gene056610 | MS.gene34412 | -0.831284 | 1.82E-55 | -1.69E-46 |
| MS.gene056657 | MS.gene34412 | -0.864284 | 1.36E-64 | -1.69E-46 |
| MS.gene056692 | MS.gene34412 | -0.849666 | 2.79E-60 | -1.69E-46 |
| MS.gene056761 | MS.gene34412 | 0.821356 | 4.22E-53 | -1.69E-46 |
| MS.gene057014 | MS.gene34412 | 0.859863 | 3.08E-63 | -1.69E-46 |
| MS.gene05726 | MS.gene34412 | 0.813094 | 3.05E-51 | -1.69E-46 |
| MS.gene057416 | MS.gene34412 | -0.815147 | 1.07E-51 | -1.69E-46 |
| MS.gene057499 | MS.gene34412 | 0.801336 | 9.45E-49 | -1.69E-46 |
| MS.gene057831 | MS.gene34412 | -0.82487 | 6.39E-54 | -1.69E-46 |
| MS.gene058068 | MS.gene34412 | 0.807217 | 5.63E-50 | -1.69E-46 |
| MS.gene058157 | MS.gene34412 | -0.805935 | 1.05E-49 | -1.69E-46 |
| MS.gene058268 | MS.gene34412 | 0.80297 | 4.36E-49 | -1.69E-46 |
| MS.gene058269 | MS.gene34412 | 0.809059 | 2.28E-50 | -1.69E-46 |
| MS.gene058338 | MS.gene34412 | -0.817051 | 4.03E-52 | -1.69E-46 |
| MS.gene05837 | MS.gene34412 | -0.848335 | 6.54E-60 | -1.69E-46 |
| MS.gene058419 | MS.gene34412 | -0.864789 | 9.44E-65 | -1.69E-46 |
| MS.gene058493 | MS.gene34412 | 0.82084 | 5.54E-53 | -1.69E-46 |
| MS.gene058494 | MS.gene34412 | 0.815645 | 8.31E-52 | -1.69E-46 |
| MS.gene058544 | MS.gene34412 | -0.846968 | 1.56E-59 | -1.69E-46 |
| MS.gene058723 | MS.gene34412 | -0.805612 | 1.23E-49 | -1.69E-46 |
| MS.gene058773 | MS.gene34412 | -0.855305 | 6.90E-62 | -1.69E-46 |
| MS.gene058838 | MS.gene34412 | -0.808338 | 3.25E-50 | -1.69E-46 |
| MS.gene05883 | MS.gene34412 | -0.816146 | 6.43E-52 | -1.69E-46 |
| MS.gene058978 | MS.gene34412 | 0.813703 | 2.24E-51 | -1.69E-46 |
| MS.gene059022 | MS.gene34412 | -0.907541 | 4.54E-81 | -1.69E-46 |
| MS.gene059023 | MS.gene34412 | -0.849718 | 2.70E-60 | -1.69E-46 |
| MS.gene059029 | MS.gene34412 | -0.80398 | 2.69E-49 | -1.69E-46 |
| MS.gene059052 | MS.gene34412 | 0.832201 | 1.08E-55 | -1.69E-46 |
| MS.gene059223 | MS.gene34412 | 0.807942 | 3.95E-50 | -1.69E-46 |
| MS.gene059677 | MS.gene34412 | -0.880388 | 5.72E-70 | -1.69E-46 |
| MS.gene059718 | MS.gene34412 | -0.870526 | 1.37E-66 | -1.69E-46 |
| MS.gene059756 | MS.gene34412 | -0.818326 | 2.08E-52 | -1.69E-46 |
| MS.gene059801 | MS.gene34412 | -0.816932 | 4.28E-52 | -1.69E-46 |
| MS.gene059837 | MS.gene34412 | -0.821908 | 3.14E-53 | -1.69E-46 |
| MS.gene059865 | MS.gene34412 | -0.862173 | 6.11E-64 | -1.69E-46 |
| MS.gene059902 | MS.gene34412 | -0.829159 | 6.02E-55 | -1.69E-46 |
| MS.gene059916 | MS.gene34412 | 0.819808 | 9.56E-53 | -1.69E-46 |
| MS.gene06000 | MS.gene34412 | -0.806427 | 8.27E-50 | -1.69E-46 |
| MS.gene060337 | MS.gene34412 | 0.818074 | 2.37E-52 | -1.69E-46 |
| MS.gene060482 | MS.gene34412 | -0.826481 | 2.65E-54 | -1.69E-46 |
| MS.gene060595 | MS.gene34412 | 0.824403 | 8.22E-54 | -1.69E-46 |
| MS.gene060602 | MS.gene34412 | 0.821471 | 3.97E-53 | -1.69E-46 |
| MS.gene060703 | MS.gene34412 | -0.873676 | 1.22E-67 | -1.69E-46 |
| MS.gene060762 | MS.gene34412 | -0.850447 | 1.69E-60 | -1.69E-46 |
| MS.gene060778 | MS.gene34412 | -0.833621 | 4.80E-56 | -1.69E-46 |
| MS.gene06077 | MS.gene34412 | -0.902871 | 6.22E-79 | -1.69E-46 |
| MS.gene06092 | MS.gene34412 | -0.821098 | 4.84E-53 | -1.69E-46 |
| MS.gene060982 | MS.gene34412 | -0.845805 | 3.23E-59 | -1.69E-46 |
| MS.gene061150 | MS.gene34412 | -0.839131 | 1.90E-57 | -1.69E-46 |
| MS.gene061226 | MS.gene34412 | 0.812484 | 4.14E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34412.t1 | MTR_1g102510 | 86.260 | 262 | 9 | 2 | 1 | 235 | 1 | 262 | 1.92e-153 | 427 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34412.t1 | AT5G51680 | 29.080 | 337 | 131 | 11 | 4 | 235 | 10 | 343 | 5.35e-15 | 73.6 |
Find 52 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACCTTCAATTCCTTTCATT+TGG | 0.224391 | 1.2:-76318867 | MS.gene34412:CDS |
| AAGAAAGGAAGAAACATATT+AGG | 0.233439 | 1.2:-76318894 | MS.gene34412:CDS |
| TTGCCTCAATTCCCTTTGTT+TGG | 0.279099 | 1.2:-76318759 | MS.gene34412:CDS |
| TGAAAAGTTAAGTTCAAGTT+TGG | 0.340393 | 1.2:-76318401 | MS.gene34412:CDS |
| ACCTTCAATTCCTTTCATTT+GGG | 0.345813 | 1.2:-76318866 | MS.gene34412:CDS |
| TATCAAAACTAAATGATTCT+AGG | 0.360427 | 1.2:+76318665 | None:intergenic |
| ACCACGAGAAAGCAAGCTTT+TGG | 0.370273 | 1.2:-76317932 | MS.gene34412:CDS |
| TGCCTCAATTCCCTTTGTTT+GGG | 0.372035 | 1.2:-76318758 | MS.gene34412:CDS |
| AAGCAACTCAAATGAACAAA+TGG | 0.378323 | 1.2:-76318264 | MS.gene34412:CDS |
| TTCAAGTTTGGTGGAACTAA+AGG | 0.388380 | 1.2:-76318389 | MS.gene34412:CDS |
| AACTGAGTTAAATGATTCAT+TGG | 0.396407 | 1.2:+76318642 | None:intergenic |
| AAACTAGGGAGGGGTATTCC+AGG | 0.409764 | 1.2:+76318727 | None:intergenic |
| TTCTAGGTCCAAGCTAGTTA+TGG | 0.418005 | 1.2:+76318681 | None:intergenic |
| AGAAAGGAAGAAACATATTA+GGG | 0.423493 | 1.2:-76318893 | MS.gene34412:CDS |
| ATGGAACAAGTTACCTTTGG+TGG | 0.430078 | 1.2:+76318238 | None:intergenic |
| TCAAGTTTGGTGGAACTAAA+GGG | 0.439502 | 1.2:-76318388 | MS.gene34412:CDS |
| CAGGCATTCCTATAAAAGAT+TGG | 0.447195 | 1.2:-76318834 | MS.gene34412:CDS |
| AAAGTTAAGTTCAAGTTTGG+TGG | 0.448826 | 1.2:-76318398 | MS.gene34412:CDS |
| CTCTCCAGCTTCGTCGGAGA+TGG | 0.451790 | 1.2:-76318545 | MS.gene34412:CDS |
| TTCCCAAACAAAGGGAATTG+AGG | 0.478406 | 1.2:+76318756 | None:intergenic |
| CTTTAGACTAGTTTCAGCCT+AGG | 0.480571 | 1.2:+76317848 | None:intergenic |
| AGAGAGTTTGCAAGTAAAGA+AGG | 0.480592 | 1.2:+76318619 | None:intergenic |
| CTATCCATCTCCGACGAAGC+TGG | 0.483008 | 1.2:+76318541 | None:intergenic |
| TTTAGACTAGTTTCAGCCTA+GGG | 0.498911 | 1.2:+76317849 | None:intergenic |
| TCCCAAATGAAAGGAATTGA+AGG | 0.504204 | 1.2:+76318865 | None:intergenic |
| AGCAACTCAAATGAACAAAT+GGG | 0.509084 | 1.2:-76318263 | MS.gene34412:CDS |
| TCATCAACATGTGAGAAACT+AGG | 0.514045 | 1.2:+76318712 | None:intergenic |
| ACAAGCAGCAGTATCATTGA+AGG | 0.522761 | 1.2:-76317893 | MS.gene34412:CDS |
| TCCGACGAAGCTGGAGAGGA+TGG | 0.522845 | 1.2:+76318550 | None:intergenic |
| ATAATTAACTCTCCAAGTGT+TGG | 0.527351 | 1.2:+76318316 | None:intergenic |
| GAGGATGGAGTTTGCTGTAG+TGG | 0.531543 | 1.2:+76318565 | None:intergenic |
| TCCATCCTCTCCAGCTTCGT+CGG | 0.537624 | 1.2:-76318551 | MS.gene34412:CDS |
| TTCATTTGGGAAACAAAACC+AGG | 0.538522 | 1.2:-76318853 | MS.gene34412:CDS |
| TCAAGGAAGCAACCAACACT+TGG | 0.542435 | 1.2:-76318328 | MS.gene34412:CDS |
| TTGCTTCCTTGACATATCAC+AGG | 0.543111 | 1.2:+76318339 | None:intergenic |
| TCCAAAAGCTTGCTTTCTCG+TGG | 0.546204 | 1.2:+76317931 | None:intergenic |
| CATCAACATGTGAGAAACTA+GGG | 0.549198 | 1.2:+76318713 | None:intergenic |
| AGTATAAGCAGCTATGAGAC+CGG | 0.561394 | 1.2:-76318517 | MS.gene34412:CDS |
| ATTATACCTGTGATATGTCA+AGG | 0.573360 | 1.2:-76318345 | MS.gene34412:CDS |
| GAGTGAAACCATAACTAGCT+TGG | 0.574998 | 1.2:-76318689 | MS.gene34412:CDS |
| TGACAAAATGATGAGTCCAC+TGG | 0.586915 | 1.2:+76318808 | None:intergenic |
| TGAAGAGAAAGTACTACCCT+AGG | 0.596271 | 1.2:-76317865 | MS.gene34412:CDS |
| CATCTCCGACGAAGCTGGAG+AGG | 0.596381 | 1.2:+76318546 | None:intergenic |
| TGGAGTTTGCTGTAGTGGCA+TGG | 0.598354 | 1.2:+76318570 | None:intergenic |
| CAAATGAAAGGAATTGAAGG+TGG | 0.607064 | 1.2:+76318868 | None:intergenic |
| GAACAAATGGGATCCACCAA+AGG | 0.609336 | 1.2:-76318251 | MS.gene34412:intron |
| ACATGTGAGAAACTAGGGAG+GGG | 0.616121 | 1.2:+76318718 | None:intergenic |
| TATAAAAGATTGGAAGCCAG+TGG | 0.619766 | 1.2:-76318824 | MS.gene34412:CDS |
| AACATGTGAGAAACTAGGGA+GGG | 0.639709 | 1.2:+76318717 | None:intergenic |
| CAAGCAGCAGTATCATTGAA+GGG | 0.643098 | 1.2:-76317892 | MS.gene34412:CDS |
| TAAGCAGCTATGAGACCGGA+AGG | 0.643457 | 1.2:-76318513 | MS.gene34412:intron |
| CAACATGTGAGAAACTAGGG+AGG | 0.699559 | 1.2:+76318716 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACGTTTATTGACATAAAAA+TGG | + | chr1.2:76318538-76318557 | None:intergenic | 20.0% |
| !! | ATTCAAATTAGCATCTAAAT+TGG | - | chr1.2:76318697-76318716 | MS.gene34412:CDS | 20.0% |
| !! | TATCAAAACTAAATGATTCT+AGG | + | chr1.2:76318092-76318111 | None:intergenic | 20.0% |
| !!! | AATTGGTTACTTTTTAAGTA+TGG | - | chr1.2:76318714-76318733 | MS.gene34412:CDS | 20.0% |
| ! | AACTGAGTTAAATGATTCAT+TGG | + | chr1.2:76318115-76318134 | None:intergenic | 25.0% |
| ! | AAGAAAGGAAGAAACATATT+AGG | - | chr1.2:76317860-76317879 | MS.gene34412:CDS | 25.0% |
| ! | AGAAAGGAAGAAACATATTA+GGG | - | chr1.2:76317861-76317880 | MS.gene34412:CDS | 25.0% |
| ! | ATAATCTCATCGAGAAAAAT+TGG | + | chr1.2:76318679-76318698 | None:intergenic | 25.0% |
| ! | CTAAAACGTTGAATTAAGAT+CGG | - | chr1.2:76318596-76318615 | MS.gene34412:CDS | 25.0% |
| ! | TATGTCAATAAACGTTTCTT+TGG | - | chr1.2:76318542-76318561 | MS.gene34412:CDS | 25.0% |
| !! | TGAAAAGTTAAGTTCAAGTT+TGG | - | chr1.2:76318353-76318372 | MS.gene34412:CDS | 25.0% |
| !!! | ATTTGTTTTAGTGGAGTTTT+TGG | + | chr1.2:76317976-76317995 | None:intergenic | 25.0% |
| AAAATGGAACAAGTTACCTT+TGG | + | chr1.2:76318522-76318541 | None:intergenic | 30.0% | |
| AAGCAACTCAAATGAACAAA+TGG | - | chr1.2:76318490-76318509 | MS.gene34412:intron | 30.0% | |
| ACCTTCAATTCCTTTCATTT+GGG | - | chr1.2:76317888-76317907 | MS.gene34412:CDS | 30.0% | |
| AGCAACTCAAATGAACAAAT+GGG | - | chr1.2:76318491-76318510 | MS.gene34412:intron | 30.0% | |
| ATAATTAACTCTCCAAGTGT+TGG | + | chr1.2:76318441-76318460 | None:intergenic | 30.0% | |
| ATTATACCTGTGATATGTCA+AGG | - | chr1.2:76318409-76318428 | MS.gene34412:CDS | 30.0% | |
| ! | AAAGTTAAGTTCAAGTTTGG+TGG | - | chr1.2:76318356-76318375 | MS.gene34412:CDS | 30.0% |
| ! | GACTTTGTATTTGGAATACT+TGG | + | chr1.2:76318646-76318665 | None:intergenic | 30.0% |
| !!! | AAAGTCTGGTTTTCTTGAAA+GGG | - | chr1.2:76318317-76318336 | MS.gene34412:CDS | 30.0% |
| !!! | ATTGAGGCAATTTGTTTTAG+TGG | + | chr1.2:76317985-76318004 | None:intergenic | 30.0% |
| !!! | TTTAACTGGTTCTGCTTTTT+TGG | - | chr1.2:76318269-76318288 | MS.gene34412:CDS | 30.0% |
| AGAGAGTTTGCAAGTAAAGA+AGG | + | chr1.2:76318138-76318157 | None:intergenic | 35.0% | |
| CAAATGAAAGGAATTGAAGG+TGG | + | chr1.2:76317889-76317908 | None:intergenic | 35.0% | |
| CACCTTCAATTCCTTTCATT+TGG | - | chr1.2:76317887-76317906 | MS.gene34412:CDS | 35.0% | |
| CAGGCATTCCTATAAAAGAT+TGG | - | chr1.2:76317920-76317939 | MS.gene34412:CDS | 35.0% | |
| CATCAACATGTGAGAAACTA+GGG | + | chr1.2:76318044-76318063 | None:intergenic | 35.0% | |
| CTTTCAAGAAAACCAGACTT+TGG | + | chr1.2:76318318-76318337 | None:intergenic | 35.0% | |
| TATAAAAGATTGGAAGCCAG+TGG | - | chr1.2:76317930-76317949 | MS.gene34412:CDS | 35.0% | |
| TCATCAACATGTGAGAAACT+AGG | + | chr1.2:76318045-76318064 | None:intergenic | 35.0% | |
| TCCCAAATGAAAGGAATTGA+AGG | + | chr1.2:76317892-76317911 | None:intergenic | 35.0% | |
| TCGTAGTCTGACTTTGTATT+TGG | + | chr1.2:76318655-76318674 | None:intergenic | 35.0% | |
| TGAAGCAGAAGAGTAAATGT+TGG | + | chr1.2:76318791-76318810 | None:intergenic | 35.0% | |
| TTCATTTGGGAAACAAAACC+AGG | - | chr1.2:76317901-76317920 | MS.gene34412:CDS | 35.0% | |
| TTTGTTTGGGAAGAAAAACC+TGG | - | chr1.2:76318009-76318028 | MS.gene34412:intron | 35.0% | |
| ! | AGGTTTTTCTTCCCAAACAA+AGG | + | chr1.2:76318010-76318029 | None:intergenic | 35.0% |
| ! | CAATCTTTTATAGGAATGCC+TGG | + | chr1.2:76317922-76317941 | None:intergenic | 35.0% |
| ! | GGTTTTCTTGAAAGGGATAT+TGG | - | chr1.2:76318324-76318343 | MS.gene34412:CDS | 35.0% |
| ! | GGTTTTTCTTCCCAAACAAA+GGG | + | chr1.2:76318009-76318028 | None:intergenic | 35.0% |
| ! | TCAAGTTTGGTGGAACTAAA+GGG | - | chr1.2:76318366-76318385 | MS.gene34412:CDS | 35.0% |
| ! | TTCAAGTTTGGTGGAACTAA+AGG | - | chr1.2:76318365-76318384 | MS.gene34412:CDS | 35.0% |
| !! | ACTGGCTTCCAATCTTTTAT+AGG | + | chr1.2:76317931-76317950 | None:intergenic | 35.0% |
| !!! | CAAAGTCTGGTTTTCTTGAA+AGG | - | chr1.2:76318316-76318335 | MS.gene34412:CDS | 35.0% |
| !!! | GGTTTTGTTTCCCAAATGAA+AGG | + | chr1.2:76317901-76317920 | None:intergenic | 35.0% |
| AACATGTGAGAAACTAGGGA+GGG | + | chr1.2:76318040-76318059 | None:intergenic | 40.0% | |
| ACAAGCAGCAGTATCATTGA+AGG | - | chr1.2:76318861-76318880 | MS.gene34412:CDS | 40.0% | |
| ACCAGTTAAACTTGACCTTC+CGG | + | chr1.2:76318259-76318278 | None:intergenic | 40.0% | |
| AGTATAAGCAGCTATGAGAC+CGG | - | chr1.2:76318237-76318256 | MS.gene34412:intron | 40.0% | |
| ATGGAACAAGTTACCTTTGG+TGG | + | chr1.2:76318519-76318538 | None:intergenic | 40.0% | |
| CAAGCAGCAGTATCATTGAA+GGG | - | chr1.2:76318862-76318881 | MS.gene34412:CDS | 40.0% | |
| GAGTGAAACCATAACTAGCT+TGG | - | chr1.2:76318065-76318084 | MS.gene34412:intron | 40.0% | |
| TCAAGAAAACCAGACTTTGG+TGG | + | chr1.2:76318315-76318334 | None:intergenic | 40.0% | |
| TGACAAAATGATGAGTCCAC+TGG | + | chr1.2:76317949-76317968 | None:intergenic | 40.0% | |
| TTCCCAAACAAAGGGAATTG+AGG | + | chr1.2:76318001-76318020 | None:intergenic | 40.0% | |
| TTCTAGGTCCAAGCTAGTTA+TGG | + | chr1.2:76318076-76318095 | None:intergenic | 40.0% | |
| TTGCCTCAATTCCCTTTGTT+TGG | - | chr1.2:76317995-76318014 | MS.gene34412:intron | 40.0% | |
| TTGCTTCCTTGACATATCAC+AGG | + | chr1.2:76318418-76318437 | None:intergenic | 40.0% | |
| ! | TGCCTCAATTCCCTTTGTTT+GGG | - | chr1.2:76317996-76318015 | MS.gene34412:intron | 40.0% |
| !! | TGAAGAGAAAGTACTACCCT+AGG | - | chr1.2:76318889-76318908 | MS.gene34412:CDS | 40.0% |
| ACATGTGAGAAACTAGGGAG+GGG | + | chr1.2:76318039-76318058 | None:intergenic | 45.0% | |
| ACCGGAAGGTCAAGTTTAAC+TGG | - | chr1.2:76318255-76318274 | MS.gene34412:CDS | 45.0% | |
| CAACATGTGAGAAACTAGGG+AGG | + | chr1.2:76318041-76318060 | None:intergenic | 45.0% | |
| CCAGACTTTGGTGGAAACAA+AGG | + | chr1.2:76318306-76318325 | None:intergenic | 45.0% | |
| GAACAAATGGGATCCACCAA+AGG | - | chr1.2:76318503-76318522 | MS.gene34412:intron | 45.0% | |
| TCAAGGAAGCAACCAACACT+TGG | - | chr1.2:76318426-76318445 | MS.gene34412:intron | 45.0% | |
| ! | ACCACGAGAAAGCAAGCTTT+TGG | - | chr1.2:76318822-76318841 | MS.gene34412:CDS | 45.0% |
| ! | CCTTTGTTTCCACCAAAGTC+TGG | - | chr1.2:76318303-76318322 | MS.gene34412:CDS | 45.0% |
| ! | TCCAAAAGCTTGCTTTCTCG+TGG | + | chr1.2:76318826-76318845 | None:intergenic | 45.0% |
| TAAGCAGCTATGAGACCGGA+AGG | - | chr1.2:76318241-76318260 | MS.gene34412:intron | 50.0% | |
| !! | AAACTAGGGAGGGGTATTCC+AGG | + | chr1.2:76318030-76318049 | None:intergenic | 50.0% |
| !! | GAGGATGGAGTTTGCTGTAG+TGG | + | chr1.2:76318192-76318211 | None:intergenic | 50.0% |
| !! | TGGAGTTTGCTGTAGTGGCA+TGG | + | chr1.2:76318187-76318206 | None:intergenic | 50.0% |
| CTATCCATCTCCGACGAAGC+TGG | + | chr1.2:76318216-76318235 | None:intergenic | 55.0% | |
| TCCATCCTCTCCAGCTTCGT+CGG | - | chr1.2:76318203-76318222 | MS.gene34412:intron | 55.0% | |
| CATCTCCGACGAAGCTGGAG+AGG | + | chr1.2:76318211-76318230 | None:intergenic | 60.0% | |
| CTCTCCAGCTTCGTCGGAGA+TGG | - | chr1.2:76318209-76318228 | MS.gene34412:intron | 60.0% | |
| TCCGACGAAGCTGGAGAGGA+TGG | + | chr1.2:76318207-76318226 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 76317850 | 76318926 | 76317850 | ID=MS.gene34412 |
| chr1.2 | mRNA | 76317850 | 76318926 | 76317850 | ID=MS.gene34412.t1;Parent=MS.gene34412 |
| chr1.2 | exon | 76318514 | 76318926 | 76318514 | ID=MS.gene34412.t1.exon1;Parent=MS.gene34412.t1 |
| chr1.2 | CDS | 76318514 | 76318926 | 76318514 | ID=cds.MS.gene34412.t1;Parent=MS.gene34412.t1 |
| chr1.2 | exon | 76318252 | 76318438 | 76318252 | ID=MS.gene34412.t1.exon2;Parent=MS.gene34412.t1 |
| chr1.2 | CDS | 76318252 | 76318438 | 76318252 | ID=cds.MS.gene34412.t1;Parent=MS.gene34412.t1 |
| chr1.2 | exon | 76317850 | 76317957 | 76317850 | ID=MS.gene34412.t1.exon3;Parent=MS.gene34412.t1 |
| chr1.2 | CDS | 76317850 | 76317957 | 76317850 | ID=cds.MS.gene34412.t1;Parent=MS.gene34412.t1 |
| Gene Sequence |
| Protein sequence |
