AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene24025


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24025.t1 MTR_7g099390 66.892 148 34 5 1 144 1 137 4.91e-59 179
MS.gene24025.t1 MTR_1g069235 92.754 138 7 1 11 145 11 148 8.63e-55 169
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24025.t1 AT2G20260 62.329 146 46 3 5 144 2 144 1.60e-44 143
MS.gene24025.t1 AT4G28750 58.621 145 50 3 5 144 2 141 9.12e-35 118

Find 50 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGGCGTAGTTCACTTTC+TGG 0.135317 1.3:-48528180 None:intergenic
CCAATATTGTTGGACTTTAA+AGG 0.153877 1.3:-48527276 None:intergenic
ATTGGTGGTGGCTTGGGTTT+AGG 0.254441 1.3:-48527396 None:intergenic
GGGGCCAATTGGTGGTGGCT+TGG 0.265521 1.3:-48527403 None:intergenic
GGGCCAATTGGTGGTGGCTT+GGG 0.275299 1.3:-48527402 None:intergenic
CAATATTGTTGGACTTTAAA+GGG 0.305571 1.3:-48527275 None:intergenic
GTGGAGGCAGCAGGTGCAGC+CGG 0.318522 1.3:-48527345 None:intergenic
CCTTTAAAGTCCAACAATAT+TGG 0.324065 1.3:+48527276 MS.gene24025:CDS
CTTCTTCTAGACTTGTTGTC+AGG 0.342347 1.3:+48527304 MS.gene24025:CDS
CTCAACAGGTGAAGATTCTT+AGG 0.381804 1.3:+48527968 MS.gene24025:intron
GAACCTCTCTTGGGGCCAAT+TGG 0.393160 1.3:-48527414 None:intergenic
CTCTTGGGGCCAATTGGTGG+TGG 0.405813 1.3:-48527408 None:intergenic
TGGCCCCAAGAGAGGTTCTA+AGG 0.412793 1.3:+48527419 MS.gene24025:CDS
ACGACAGGGTAGCGAGTCTT+GGG 0.413817 1.3:-48528148 None:intergenic
AATTACCTTAGAACCTCTCT+TGG 0.417720 1.3:-48527424 None:intergenic
AGAAGAAGAACCAATATTGT+TGG 0.421736 1.3:-48527286 None:intergenic
TGCAGCCGGGGCAGCCTCAT+CGG 0.425226 1.3:-48527331 None:intergenic
ATCGACAAATAACTATGCTT+TGG 0.426234 1.3:+48528204 MS.gene24025:CDS
CCTTGATGAAGTTGTGTTGT+TGG 0.428946 1.3:-48527235 None:intergenic
GGTGGCTTGGGTTTAGGAGT+AGG 0.430314 1.3:-48527390 None:intergenic
CCTCTCTTGGGGCCAATTGG+TGG 0.430678 1.3:-48527411 None:intergenic
GCTGGAGCAGTGGAGGCAGC+AGG 0.442829 1.3:-48527354 None:intergenic
TTCTGGAATCGAACCACGAC+AGG 0.446735 1.3:-48528163 None:intergenic
ATTACCTTAGAACCTCTCTT+GGG 0.450802 1.3:-48527423 None:intergenic
CACGACAGGGTAGCGAGTCT+TGG 0.452960 1.3:-48528149 None:intergenic
GGCAACCGATGAGGCTGCCC+CGG 0.460044 1.3:+48527326 MS.gene24025:CDS
GCTGCCTCCACTGCTCCAGC+TGG 0.461366 1.3:+48527357 MS.gene24025:CDS
AAACCCAAGCCACCACCAAT+TGG 0.463084 1.3:+48527399 MS.gene24025:CDS
GGAGGCAGCAGGTGCAGCCG+GGG 0.477518 1.3:-48527343 None:intergenic
TTCTTCTAGACTTGTTGTCA+GGG 0.490708 1.3:+48527305 MS.gene24025:CDS
GTAGGAGCAGCATCACCAGC+TGG 0.494338 1.3:-48527372 None:intergenic
TGGAGGCAGCAGGTGCAGCC+GGG 0.495138 1.3:-48527344 None:intergenic
AGCATGGCATCAGCAGCTAC+TGG 0.498675 1.3:+48527186 MS.gene24025:CDS
ATCACCAGCTGGAGCAGTGG+AGG 0.508802 1.3:-48527361 None:intergenic
AGCATCACCAGCTGGAGCAG+TGG 0.519718 1.3:-48527364 None:intergenic
TCCTACTGGTACAAAGGCAC+TGG 0.520039 1.3:+48527997 MS.gene24025:CDS
ATAGTTATTTGTCGATACAT+TGG 0.524557 1.3:-48528197 None:intergenic
TTCTTAGGAAGGAATCCTAC+TGG 0.542855 1.3:+48527983 MS.gene24025:CDS
ACAGGTGAAGATTCTTAGGA+AGG 0.576965 1.3:+48527972 MS.gene24025:intron
AAGGAATCCTACTGGTACAA+AGG 0.577304 1.3:+48527991 MS.gene24025:CDS
CCACCAATTGGCCCCAAGAG+AGG 0.582857 1.3:+48527411 MS.gene24025:CDS
CAAGACTCGCTACCCTGTCG+TGG 0.613483 1.3:+48528150 MS.gene24025:CDS
CCAACAACACAACTTCATCA+AGG 0.613961 1.3:+48527235 MS.gene24025:CDS
AGTCATGGCTAGCAGCAGCA+TGG 0.629935 1.3:+48527170 None:intergenic
TGTTGTCAGGGCAACCGATG+AGG 0.640147 1.3:+48527317 MS.gene24025:CDS
CTTTAAAGGGAACATCAACA+TGG 0.644075 1.3:-48527262 None:intergenic
TTACCTTAGAACCTCTCTTG+GGG 0.647944 1.3:-48527422 None:intergenic
TCCAGTGCCTTTGTACCAGT+AGG 0.656987 1.3:-48527998 None:intergenic
CGACAGGGTAGCGAGTCTTG+GGG 0.696532 1.3:-48528147 None:intergenic
TCTGGAATCGAACCACGACA+GGG 0.707301 1.3:-48528162 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATGAGGAAAAAACTTAA+AGG - chr1.3:48528077-48528096 None:intergenic 20.0%
!!! AAGATATATTAGAGTGTTTT+TGG + chr1.3:48527651-48527670 MS.gene24025:intron 20.0%
!!! GTTTAGTTTGAATTTACTTA+TGG + chr1.3:48527535-48527554 MS.gene24025:intron 20.0%
! ATAGTTATTTGTCGATACAT+TGG - chr1.3:48528200-48528219 None:intergenic 25.0%
! ATTTCAACTAAAACTCACTT+GGG + chr1.3:48527695-48527714 MS.gene24025:intron 25.0%
! CAATATTGTTGGACTTTAAA+GGG - chr1.3:48527278-48527297 None:intergenic 25.0%
! TAGCTAAAGATAGTTGAAAT+AGG - chr1.3:48527835-48527854 None:intergenic 25.0%
! TATTTCAACTAAAACTCACT+TGG + chr1.3:48527694-48527713 MS.gene24025:intron 25.0%
! TTCTTGTTGCAATTATACAT+TGG + chr1.3:48527513-48527532 MS.gene24025:intron 25.0%
!!! AAGTGAGTTTTAGTTGAAAT+AGG - chr1.3:48527695-48527714 None:intergenic 25.0%
AGAAGAAGAACCAATATTGT+TGG - chr1.3:48527289-48527308 None:intergenic 30.0%
ATCGACAAATAACTATGCTT+TGG + chr1.3:48528204-48528223 MS.gene24025:CDS 30.0%
CCAATATTGTTGGACTTTAA+AGG - chr1.3:48527279-48527298 None:intergenic 30.0%
GAGGAAAAAACTTAAAGGTT+TGG - chr1.3:48528072-48528091 None:intergenic 30.0%
TGATACTACTTATGACTTTG+TGG + chr1.3:48527609-48527628 MS.gene24025:intron 30.0%
! TTGGTTGAAATTTGAGTTTG+AGG - chr1.3:48528053-48528072 None:intergenic 30.0%
!! CCTTTAAAGTCCAACAATAT+TGG + chr1.3:48527276-48527295 MS.gene24025:CDS 30.0%
!!! GTTTTTTCCTCATAATCCAT+TGG + chr1.3:48528081-48528100 MS.gene24025:intron 30.0%
AATCTCCGTAGTGTCAATTT+AGG + chr1.3:48527779-48527798 MS.gene24025:intron 35.0%
AATTACCTTAGAACCTCTCT+TGG - chr1.3:48527427-48527446 None:intergenic 35.0%
ATTACCTTAGAACCTCTCTT+GGG - chr1.3:48527426-48527445 None:intergenic 35.0%
GATATTTCTTTGCACTCAAC+AGG + chr1.3:48527954-48527973 MS.gene24025:intron 35.0%
! CTATCTTTAGCTAGTCTACA+CGG + chr1.3:48527842-48527861 MS.gene24025:intron 35.0%
! CTTTAAAGGGAACATCAACA+TGG - chr1.3:48527265-48527284 None:intergenic 35.0%
! GTAACTCAGCTCTTGTTTAA+CGG + chr1.3:48528103-48528122 MS.gene24025:intron 35.0%
!! TGAGTTACCAATGGATTATG+AGG - chr1.3:48528091-48528110 None:intergenic 35.0%
!! TTCTTCTAGACTTGTTGTCA+GGG + chr1.3:48527305-48527324 MS.gene24025:CDS 35.0%
AACAAGAGCTGAGTTACCAA+TGG - chr1.3:48528100-48528119 None:intergenic 40.0%
AAGGAATCCTACTGGTACAA+AGG + chr1.3:48527991-48528010 MS.gene24025:CDS 40.0%
ACAGGTGAAGATTCTTAGGA+AGG + chr1.3:48527972-48527991 MS.gene24025:intron 40.0%
AGGTCTCAAGTCAATATCAC+CGG - chr1.3:48527726-48527745 None:intergenic 40.0%
ATAACCGGTCTAAATTGACG+AGG + chr1.3:48527885-48527904 MS.gene24025:intron 40.0%
CAACTAAAACTCACTTGGGT+TGG + chr1.3:48527699-48527718 MS.gene24025:intron 40.0%
CCAACAACACAACTTCATCA+AGG + chr1.3:48527235-48527254 MS.gene24025:CDS 40.0%
CCTTGATGAAGTTGTGTTGT+TGG - chr1.3:48527238-48527257 None:intergenic 40.0%
CTCAACAGGTGAAGATTCTT+AGG + chr1.3:48527968-48527987 MS.gene24025:intron 40.0%
GTCCACCTAAATTGACACTA+CGG - chr1.3:48527787-48527806 None:intergenic 40.0%
TAACCGGTCTAAATTGACGA+GGG + chr1.3:48527886-48527905 MS.gene24025:intron 40.0%
TTACCTTAGAACCTCTCTTG+GGG - chr1.3:48527425-48527444 None:intergenic 40.0%
TTCTTAGGAAGGAATCCTAC+TGG + chr1.3:48527983-48528002 MS.gene24025:CDS 40.0%
!! CTTCTTCTAGACTTGTTGTC+AGG + chr1.3:48527304-48527323 MS.gene24025:CDS 40.0%
!! TCGGTGATTAGTCTCATAAC+CGG + chr1.3:48527870-48527889 MS.gene24025:intron 40.0%
CATTGGCGTAGTTCACTTTC+TGG - chr1.3:48528183-48528202 None:intergenic 45.0%
CTCCGTAGTGTCAATTTAGG+TGG + chr1.3:48527782-48527801 MS.gene24025:intron 45.0%
GGTCTCAAGTCAATATCACC+GGG - chr1.3:48527725-48527744 None:intergenic 45.0%
TCACCCTCGTCAATTTAGAC+CGG - chr1.3:48527892-48527911 None:intergenic 45.0%
! CGGATTTCTCTTTGTTGTGC+AGG + chr1.3:48528123-48528142 MS.gene24025:intron 45.0%
! GCTAGTCTACACGGTCAAAT+CGG + chr1.3:48527851-48527870 MS.gene24025:intron 45.0%
AAACCCAAGCCACCACCAAT+TGG + chr1.3:48527399-48527418 MS.gene24025:CDS 50.0%
TCCAGTGCCTTTGTACCAGT+AGG - chr1.3:48528001-48528020 None:intergenic 50.0%
TCGAACCTGAGACCTTGAAG+AGG - chr1.3:48527760-48527779 None:intergenic 50.0%
! TCCTACTGGTACAAAGGCAC+TGG + chr1.3:48527997-48528016 MS.gene24025:CDS 50.0%
! TCTGGAATCGAACCACGACA+GGG - chr1.3:48528165-48528184 None:intergenic 50.0%
! TGAGAGTGTGCTCCTCTTCA+AGG + chr1.3:48527745-48527764 MS.gene24025:intron 50.0%
! TTGAAGAGGAGCACACTCTC+AGG - chr1.3:48527746-48527765 None:intergenic 50.0%
!! AAAACTCACTTGGGTTGGCC+CGG + chr1.3:48527704-48527723 MS.gene24025:intron 50.0%
!! ATTGGTGGTGGCTTGGGTTT+AGG - chr1.3:48527399-48527418 None:intergenic 50.0%
!! TTCTGGAATCGAACCACGAC+AGG - chr1.3:48528166-48528185 None:intergenic 50.0%
ACGACAGGGTAGCGAGTCTT+GGG - chr1.3:48528151-48528170 None:intergenic 55.0%
AGCATGGCATCAGCAGCTAC+TGG + chr1.3:48527186-48527205 MS.gene24025:CDS 55.0%
GAACCTCTCTTGGGGCCAAT+TGG - chr1.3:48527417-48527436 None:intergenic 55.0%
GTGCTCCTCTTCAAGGTCTC+AGG + chr1.3:48527752-48527771 MS.gene24025:intron 55.0%
TGGCCCCAAGAGAGGTTCTA+AGG + chr1.3:48527419-48527438 MS.gene24025:CDS 55.0%
TGTTGTCAGGGCAACCGATG+AGG + chr1.3:48527317-48527336 MS.gene24025:CDS 55.0%
! GGTGGCTTGGGTTTAGGAGT+AGG - chr1.3:48527393-48527412 None:intergenic 55.0%
AGCATCACCAGCTGGAGCAG+TGG - chr1.3:48527367-48527386 None:intergenic 60.0%
ATCACCAGCTGGAGCAGTGG+AGG - chr1.3:48527364-48527383 None:intergenic 60.0%
CAAGACTCGCTACCCTGTCG+TGG + chr1.3:48528150-48528169 MS.gene24025:CDS 60.0%
CACGACAGGGTAGCGAGTCT+TGG - chr1.3:48528152-48528171 None:intergenic 60.0%
CCACCAATTGGCCCCAAGAG+AGG + chr1.3:48527411-48527430 MS.gene24025:CDS 60.0%
CCTCTCTTGGGGCCAATTGG+TGG - chr1.3:48527414-48527433 None:intergenic 60.0%
CGACAGGGTAGCGAGTCTTG+GGG - chr1.3:48528150-48528169 None:intergenic 60.0%
CTCTTGGGGCCAATTGGTGG+TGG - chr1.3:48527411-48527430 None:intergenic 60.0%
GTAGGAGCAGCATCACCAGC+TGG - chr1.3:48527375-48527394 None:intergenic 60.0%
!! GGGCCAATTGGTGGTGGCTT+GGG - chr1.3:48527405-48527424 None:intergenic 60.0%
!! GGGGCCAATTGGTGGTGGCT+TGG - chr1.3:48527406-48527425 None:intergenic 65.0%
GCTGCCTCCACTGCTCCAGC+TGG + chr1.3:48527357-48527376 MS.gene24025:CDS 70.0%
GCTGGAGCAGTGGAGGCAGC+AGG - chr1.3:48527357-48527376 None:intergenic 70.0%
GGCAACCGATGAGGCTGCCC+CGG + chr1.3:48527326-48527345 MS.gene24025:CDS 70.0%
GTGGAGGCAGCAGGTGCAGC+CGG - chr1.3:48527348-48527367 None:intergenic 70.0%
TGCAGCCGGGGCAGCCTCAT+CGG - chr1.3:48527334-48527353 None:intergenic 70.0%
TGGAGGCAGCAGGTGCAGCC+GGG - chr1.3:48527347-48527366 None:intergenic 70.0%
GGAGGCAGCAGGTGCAGCCG+GGG - chr1.3:48527346-48527365 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 48527174 48528249 48527174 ID=MS.gene24025
chr1.3 mRNA 48527174 48528249 48527174 ID=MS.gene24025.t1;Parent=MS.gene24025
chr1.3 exon 48527174 48527440 48527174 ID=MS.gene24025.t1.exon1;Parent=MS.gene24025.t1
chr1.3 CDS 48527174 48527440 48527174 ID=cds.MS.gene24025.t1;Parent=MS.gene24025.t1
chr1.3 exon 48527976 48528041 48527976 ID=MS.gene24025.t1.exon2;Parent=MS.gene24025.t1
chr1.3 CDS 48527976 48528041 48527976 ID=cds.MS.gene24025.t1;Parent=MS.gene24025.t1
chr1.3 exon 48528145 48528249 48528145 ID=MS.gene24025.t1.exon3;Parent=MS.gene24025.t1
chr1.3 CDS 48528145 48528249 48528145 ID=cds.MS.gene24025.t1;Parent=MS.gene24025.t1
Gene Sequence

>MS.gene24025

ATGGCTAGCAGCAGCATGGCATCAGCAGCTACTGGATTTTTGTTGTCACTTAATGTTGCTTCCAACAACACAACTTCATCAAGGAGTACCATGTTGATGTTCCCTTTAAAGTCCAACAATATTGGTTCTTCTTCTTCTAGACTTGTTGTCAGGGCAACCGATGAGGCTGCCCCGGCTGCACCTGCTGCCTCCACTGCTCCAGCTGGTGATGCTGCTCCTACTCCTAAACCCAAGCCACCACCAATTGGCCCCAAGAGAGGTTCTAAGGTAATTCATTTCATTATCATATTAAGATAGACTACATAGAATTATATATTTAAAATTTTGTATCAAAGTAGTTTCTTGTTGCAATTATACATTGGTTTAGTTTGAATTTACTTATGGTGTATCAAAGCTTTTCAATTCAAATTAATTTATGATTCTTCATTTATAAAATGATACTACTTATGACTTTGTGGATTCATAGATCTTCATGAAAAGATATATTAGAGTGTTTTTGGAAACATTAAGATGCATAACCTATTTCAACTAAAACTCACTTGGGTTGGCCCGGTGATATTGACTTGAGACCTGAGAGTGTGCTCCTCTTCAAGGTCTCAGGTTCGAATCTCCGTAGTGTCAATTTAGGTGGACTAGTTTAGTTTCTTAAAAAAAATTACCTATTTCAACTATCTTTAGCTAGTCTACACGGTCAAATCGGTGATTAGTCTCATAACCGGTCTAAATTGACGAGGGTGAAAATTATATTGAACTAGCTCTCTAGCAACAAGTATAGCTTATGATATTTCTTTGCACTCAACAGGTGAAGATTCTTAGGAAGGAATCCTACTGGTACAAAGGCACTGGATCAGTTGTTGCTGTTGATCAAGTGAGTGACCTCAAACTCAAATTTCAACCAAACCTTTAAGTTTTTTCCTCATAATCCATTGGTAACTCAGCTCTTGTTTAACGGATTTCTCTTTGTTGTGCAGGACCCCAAGACTCGCTACCCTGTCGTGGTTCGATTCCAGAAAGTGAACTACGCCAATGTATCGACAAATAACTATGCTTTGGATGAGATTGAAGAAGTTGTTTAA

Protein sequence

>MS.gene24025.t1

MASSSMASAATGFLLSLNVASNNTTSSRSTMLMFPLKSNNIGSSSSRLVVRATDEAAPAAPAASTAPAGDAAPTPKPKPPPIGPKRGSKVKILRKESYWYKGTGSVVAVDQDPKTRYPVVVRFQKVNYANVSTNNYALDEIEEVV