AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene94806


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94806.t1 MTR_1g069235 95.270 148 4 1 1 145 1 148 5.56e-97 276
MS.gene94806.t1 MTR_7g099390 66.216 148 35 5 1 144 1 137 1.30e-58 178
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94806.t1 AT2G20260 63.014 146 45 3 5 144 2 144 5.86e-45 144
MS.gene94806.t1 AT4G28750 57.931 145 51 3 5 144 2 141 8.82e-35 118

Find 50 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGGCGTAGTTCACTTTC+TGG 0.135317 1.4:-55676969 None:intergenic
CCAATATTGTTGGACTTTAA+AGG 0.153877 1.4:-55676065 None:intergenic
ATTGGTGGTGGCTTGGGTTT+AGG 0.254441 1.4:-55676185 None:intergenic
GGGGCCAATTGGTGGTGGCT+TGG 0.265521 1.4:-55676192 None:intergenic
GGGCCAATTGGTGGTGGCTT+GGG 0.275299 1.4:-55676191 None:intergenic
CAATATTGTTGGACTTTAAA+GGG 0.305571 1.4:-55676064 None:intergenic
GTGGAGGCAGCAGGTGCAGC+CGG 0.318522 1.4:-55676134 None:intergenic
CCTTTAAAGTCCAACAATAT+TGG 0.324065 1.4:+55676065 MS.gene94806:CDS
CTTCTTCTAGACTTGTTGTC+AGG 0.342347 1.4:+55676093 MS.gene94806:CDS
CTCAACAGGTGAAGATTCTT+AGG 0.381804 1.4:+55676757 MS.gene94806:intron
GAACCTCTCTTGGGGCCAAT+TGG 0.393160 1.4:-55676203 None:intergenic
CTCTTGGGGCCAATTGGTGG+TGG 0.405813 1.4:-55676197 None:intergenic
CCTTGATGAAGTTGTGTTGT+TGG 0.411874 1.4:-55676024 None:intergenic
TGGCCCCAAGAGAGGTTCTA+AGG 0.412793 1.4:+55676208 MS.gene94806:CDS
ACGACAGGGTAGCGAGTCTT+GGG 0.413817 1.4:-55676937 None:intergenic
AATTACCTTAGAACCTCTCT+TGG 0.417720 1.4:-55676213 None:intergenic
AGAAGAAGAACCAATATTGT+TGG 0.421736 1.4:-55676075 None:intergenic
TGCAGCCGGGGCAGCCTCAT+CGG 0.425226 1.4:-55676120 None:intergenic
ATCGACAAATAACTATGCTT+TGG 0.426234 1.4:+55676993 MS.gene94806:CDS
GGTGGCTTGGGTTTAGGAGT+AGG 0.430314 1.4:-55676179 None:intergenic
CCTCTCTTGGGGCCAATTGG+TGG 0.430678 1.4:-55676200 None:intergenic
GCTGGAGCAGTGGAGGCAGC+AGG 0.442829 1.4:-55676143 None:intergenic
TTCTGGAATCGAACCACGAC+AGG 0.446735 1.4:-55676952 None:intergenic
ATTACCTTAGAACCTCTCTT+GGG 0.450802 1.4:-55676212 None:intergenic
CACGACAGGGTAGCGAGTCT+TGG 0.452960 1.4:-55676938 None:intergenic
GGCAACCGATGAGGCTGCCC+CGG 0.460044 1.4:+55676115 MS.gene94806:CDS
GCTGCCTCCACTGCTCCAGC+TGG 0.461366 1.4:+55676146 MS.gene94806:CDS
AAACCCAAGCCACCACCAAT+TGG 0.463084 1.4:+55676188 MS.gene94806:CDS
GGAGGCAGCAGGTGCAGCCG+GGG 0.477518 1.4:-55676132 None:intergenic
TTCTTCTAGACTTGTTGTCA+GGG 0.490708 1.4:+55676094 MS.gene94806:CDS
GTAGGAGCAGCATCACCAGC+TGG 0.494338 1.4:-55676161 None:intergenic
TGGAGGCAGCAGGTGCAGCC+GGG 0.495138 1.4:-55676133 None:intergenic
AGCATGGCATCAGCAGCTAC+TGG 0.498675 1.4:+55675975 MS.gene94806:CDS
ATCACCAGCTGGAGCAGTGG+AGG 0.508802 1.4:-55676150 None:intergenic
AGCATCACCAGCTGGAGCAG+TGG 0.519718 1.4:-55676153 None:intergenic
TCCTACTGGTACAAAGGCAC+TGG 0.520039 1.4:+55676786 MS.gene94806:CDS
ATAGTTATTTGTCGATACAT+TGG 0.524557 1.4:-55676986 None:intergenic
TTCTTAGGAAGGAATCCTAC+TGG 0.542855 1.4:+55676772 MS.gene94806:CDS
ACAGGTGAAGATTCTTAGGA+AGG 0.576965 1.4:+55676761 MS.gene94806:intron
AAGGAATCCTACTGGTACAA+AGG 0.577304 1.4:+55676780 MS.gene94806:CDS
CCACCAATTGGCCCCAAGAG+AGG 0.582857 1.4:+55676200 MS.gene94806:CDS
CAAGACTCGCTACCCTGTCG+TGG 0.613483 1.4:+55676939 MS.gene94806:CDS
CCAACAACACAACTTCATCA+AGG 0.613961 1.4:+55676024 MS.gene94806:CDS
AGTCATGGCTAGCAGCAGCA+TGG 0.629935 1.4:+55675959 None:intergenic
TGTTGTCAGGGCAACCGATG+AGG 0.640147 1.4:+55676106 MS.gene94806:CDS
CTTTAAAGGGAACATCAACA+TGG 0.644075 1.4:-55676051 None:intergenic
TTACCTTAGAACCTCTCTTG+GGG 0.647944 1.4:-55676211 None:intergenic
TCCAGTGCCTTTGTACCAGT+AGG 0.656987 1.4:-55676787 None:intergenic
CGACAGGGTAGCGAGTCTTG+GGG 0.696532 1.4:-55676936 None:intergenic
TCTGGAATCGAACCACGACA+GGG 0.707301 1.4:-55676951 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATGAGGAAAAAACTTAA+AGG - chr1.4:55676866-55676885 None:intergenic 20.0%
!!! AAGATATATTAGAGTGTTTT+TGG + chr1.4:55676440-55676459 MS.gene94806:intron 20.0%
!!! GTTTAGTTTGAATTTACTTA+TGG + chr1.4:55676324-55676343 MS.gene94806:intron 20.0%
! ATAGTTATTTGTCGATACAT+TGG - chr1.4:55676989-55677008 None:intergenic 25.0%
! ATTTCAACTAAAACTCACTT+GGG + chr1.4:55676484-55676503 MS.gene94806:intron 25.0%
! CAATATTGTTGGACTTTAAA+GGG - chr1.4:55676067-55676086 None:intergenic 25.0%
! TAGCTAAAGATAGTTGAAAT+AGG - chr1.4:55676624-55676643 None:intergenic 25.0%
! TATTTCAACTAAAACTCACT+TGG + chr1.4:55676483-55676502 MS.gene94806:intron 25.0%
! TTCTTGTTGCAATTATACAT+TGG + chr1.4:55676302-55676321 MS.gene94806:intron 25.0%
!!! AAGTGAGTTTTAGTTGAAAT+AGG - chr1.4:55676484-55676503 None:intergenic 25.0%
AGAAGAAGAACCAATATTGT+TGG - chr1.4:55676078-55676097 None:intergenic 30.0%
ATCGACAAATAACTATGCTT+TGG + chr1.4:55676993-55677012 MS.gene94806:CDS 30.0%
CCAATATTGTTGGACTTTAA+AGG - chr1.4:55676068-55676087 None:intergenic 30.0%
GAGGAAAAAACTTAAAGGTT+TGG - chr1.4:55676861-55676880 None:intergenic 30.0%
TGATACTACTTATGACTTTG+TGG + chr1.4:55676398-55676417 MS.gene94806:intron 30.0%
! TTGGTTGAAATTTGAGTTTG+AGG - chr1.4:55676842-55676861 None:intergenic 30.0%
!! CCTTTAAAGTCCAACAATAT+TGG + chr1.4:55676065-55676084 MS.gene94806:CDS 30.0%
!!! GTTTTTTCCTCATAATCCAT+TGG + chr1.4:55676870-55676889 MS.gene94806:intron 30.0%
AATCTCCGTAGTGTCAATTT+AGG + chr1.4:55676568-55676587 MS.gene94806:intron 35.0%
AATTACCTTAGAACCTCTCT+TGG - chr1.4:55676216-55676235 None:intergenic 35.0%
ATTACCTTAGAACCTCTCTT+GGG - chr1.4:55676215-55676234 None:intergenic 35.0%
GATATTTCTTTGCACTCAAC+AGG + chr1.4:55676743-55676762 MS.gene94806:intron 35.0%
! CTATCTTTAGCTAGTCTACA+CGG + chr1.4:55676631-55676650 MS.gene94806:intron 35.0%
! CTTTAAAGGGAACATCAACA+TGG - chr1.4:55676054-55676073 None:intergenic 35.0%
! GTAACTCAGCTCTTGTTTAA+CGG + chr1.4:55676892-55676911 MS.gene94806:intron 35.0%
!! TGAGTTACCAATGGATTATG+AGG - chr1.4:55676880-55676899 None:intergenic 35.0%
!! TTCTTCTAGACTTGTTGTCA+GGG + chr1.4:55676094-55676113 MS.gene94806:CDS 35.0%
AACAAGAGCTGAGTTACCAA+TGG - chr1.4:55676889-55676908 None:intergenic 40.0%
AAGGAATCCTACTGGTACAA+AGG + chr1.4:55676780-55676799 MS.gene94806:CDS 40.0%
ACAGGTGAAGATTCTTAGGA+AGG + chr1.4:55676761-55676780 MS.gene94806:intron 40.0%
AGGTCTCAAGTCAATATCAC+CGG - chr1.4:55676515-55676534 None:intergenic 40.0%
ATAACCGGTCTAAATTGACG+AGG + chr1.4:55676674-55676693 MS.gene94806:intron 40.0%
CAACTAAAACTCACTTGGGT+TGG + chr1.4:55676488-55676507 MS.gene94806:intron 40.0%
CCAACAACACAACTTCATCA+AGG + chr1.4:55676024-55676043 MS.gene94806:CDS 40.0%
CCTTGATGAAGTTGTGTTGT+TGG - chr1.4:55676027-55676046 None:intergenic 40.0%
CTCAACAGGTGAAGATTCTT+AGG + chr1.4:55676757-55676776 MS.gene94806:intron 40.0%
GTCCACCTAAATTGACACTA+CGG - chr1.4:55676576-55676595 None:intergenic 40.0%
TAACCGGTCTAAATTGACGA+GGG + chr1.4:55676675-55676694 MS.gene94806:intron 40.0%
TTACCTTAGAACCTCTCTTG+GGG - chr1.4:55676214-55676233 None:intergenic 40.0%
TTCTTAGGAAGGAATCCTAC+TGG + chr1.4:55676772-55676791 MS.gene94806:CDS 40.0%
!! CGGATTTTTCTTTGTTGTGC+AGG + chr1.4:55676912-55676931 MS.gene94806:intron 40.0%
!! CTTCTTCTAGACTTGTTGTC+AGG + chr1.4:55676093-55676112 MS.gene94806:CDS 40.0%
!! TCGGTGATTAGTCTCATAAC+CGG + chr1.4:55676659-55676678 MS.gene94806:intron 40.0%
CATTGGCGTAGTTCACTTTC+TGG - chr1.4:55676972-55676991 None:intergenic 45.0%
CTCCGTAGTGTCAATTTAGG+TGG + chr1.4:55676571-55676590 MS.gene94806:intron 45.0%
GGTCTCAAGTCAATATCACC+GGG - chr1.4:55676514-55676533 None:intergenic 45.0%
TCACCCTCGTCAATTTAGAC+CGG - chr1.4:55676681-55676700 None:intergenic 45.0%
! GCTAGTCTACACGGTCAAAT+CGG + chr1.4:55676640-55676659 MS.gene94806:intron 45.0%
AAACCCAAGCCACCACCAAT+TGG + chr1.4:55676188-55676207 MS.gene94806:CDS 50.0%
TCCAGTGCCTTTGTACCAGT+AGG - chr1.4:55676790-55676809 None:intergenic 50.0%
TCGAACCTGAGACCTTGAAG+AGG - chr1.4:55676549-55676568 None:intergenic 50.0%
! TCCTACTGGTACAAAGGCAC+TGG + chr1.4:55676786-55676805 MS.gene94806:CDS 50.0%
! TCTGGAATCGAACCACGACA+GGG - chr1.4:55676954-55676973 None:intergenic 50.0%
! TGAGAGTGTGCTCCTCTTCA+AGG + chr1.4:55676534-55676553 MS.gene94806:intron 50.0%
! TTGAAGAGGAGCACACTCTC+AGG - chr1.4:55676535-55676554 None:intergenic 50.0%
!! AAAACTCACTTGGGTTGGCC+CGG + chr1.4:55676493-55676512 MS.gene94806:intron 50.0%
!! ATTGGTGGTGGCTTGGGTTT+AGG - chr1.4:55676188-55676207 None:intergenic 50.0%
!! TTCTGGAATCGAACCACGAC+AGG - chr1.4:55676955-55676974 None:intergenic 50.0%
ACGACAGGGTAGCGAGTCTT+GGG - chr1.4:55676940-55676959 None:intergenic 55.0%
AGCATGGCATCAGCAGCTAC+TGG + chr1.4:55675975-55675994 MS.gene94806:CDS 55.0%
GAACCTCTCTTGGGGCCAAT+TGG - chr1.4:55676206-55676225 None:intergenic 55.0%
GTGCTCCTCTTCAAGGTCTC+AGG + chr1.4:55676541-55676560 MS.gene94806:intron 55.0%
TGGCCCCAAGAGAGGTTCTA+AGG + chr1.4:55676208-55676227 MS.gene94806:CDS 55.0%
TGTTGTCAGGGCAACCGATG+AGG + chr1.4:55676106-55676125 MS.gene94806:CDS 55.0%
! GGTGGCTTGGGTTTAGGAGT+AGG - chr1.4:55676182-55676201 None:intergenic 55.0%
AGCATCACCAGCTGGAGCAG+TGG - chr1.4:55676156-55676175 None:intergenic 60.0%
ATCACCAGCTGGAGCAGTGG+AGG - chr1.4:55676153-55676172 None:intergenic 60.0%
CAAGACTCGCTACCCTGTCG+TGG + chr1.4:55676939-55676958 MS.gene94806:CDS 60.0%
CACGACAGGGTAGCGAGTCT+TGG - chr1.4:55676941-55676960 None:intergenic 60.0%
CCACCAATTGGCCCCAAGAG+AGG + chr1.4:55676200-55676219 MS.gene94806:CDS 60.0%
CCTCTCTTGGGGCCAATTGG+TGG - chr1.4:55676203-55676222 None:intergenic 60.0%
CGACAGGGTAGCGAGTCTTG+GGG - chr1.4:55676939-55676958 None:intergenic 60.0%
CTCTTGGGGCCAATTGGTGG+TGG - chr1.4:55676200-55676219 None:intergenic 60.0%
GTAGGAGCAGCATCACCAGC+TGG - chr1.4:55676164-55676183 None:intergenic 60.0%
!! GGGCCAATTGGTGGTGGCTT+GGG - chr1.4:55676194-55676213 None:intergenic 60.0%
!! GGGGCCAATTGGTGGTGGCT+TGG - chr1.4:55676195-55676214 None:intergenic 65.0%
GCTGCCTCCACTGCTCCAGC+TGG + chr1.4:55676146-55676165 MS.gene94806:CDS 70.0%
GCTGGAGCAGTGGAGGCAGC+AGG - chr1.4:55676146-55676165 None:intergenic 70.0%
GGCAACCGATGAGGCTGCCC+CGG + chr1.4:55676115-55676134 MS.gene94806:CDS 70.0%
GTGGAGGCAGCAGGTGCAGC+CGG - chr1.4:55676137-55676156 None:intergenic 70.0%
TGCAGCCGGGGCAGCCTCAT+CGG - chr1.4:55676123-55676142 None:intergenic 70.0%
TGGAGGCAGCAGGTGCAGCC+GGG - chr1.4:55676136-55676155 None:intergenic 70.0%
GGAGGCAGCAGGTGCAGCCG+GGG - chr1.4:55676135-55676154 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 55675963 55677038 55675963 ID=MS.gene94806
chr1.4 mRNA 55675963 55677038 55675963 ID=MS.gene94806.t1;Parent=MS.gene94806
chr1.4 exon 55675963 55676229 55675963 ID=MS.gene94806.t1.exon1;Parent=MS.gene94806.t1
chr1.4 CDS 55675963 55676229 55675963 ID=cds.MS.gene94806.t1;Parent=MS.gene94806.t1
chr1.4 exon 55676765 55676830 55676765 ID=MS.gene94806.t1.exon2;Parent=MS.gene94806.t1
chr1.4 CDS 55676765 55676830 55676765 ID=cds.MS.gene94806.t1;Parent=MS.gene94806.t1
chr1.4 exon 55676934 55677038 55676934 ID=MS.gene94806.t1.exon3;Parent=MS.gene94806.t1
chr1.4 CDS 55676934 55677038 55676934 ID=cds.MS.gene94806.t1;Parent=MS.gene94806.t1
Gene Sequence

>MS.gene94806

ATGGCTAGCAGCAGCATGGCATCAGCAGCTACTGGATTTTTGTTGTCACTTAATGTTGCTGCCAACAACACAACTTCATCAAGGAGTACCATGTTGATGTTCCCTTTAAAGTCCAACAATATTGGTTCTTCTTCTTCTAGACTTGTTGTCAGGGCAACCGATGAGGCTGCCCCGGCTGCACCTGCTGCCTCCACTGCTCCAGCTGGTGATGCTGCTCCTACTCCTAAACCCAAGCCACCACCAATTGGCCCCAAGAGAGGTTCTAAGGTAATTCATTTCATTATCATATTAAGATAGACTACATAGACTTATATATTTAAAATTTTGTATCAAAGTAGTTTCTTGTTGCAATTATACATTGGTTTAGTTTGAATTTACTTATGGTGTATCAAAGCTTTTCAATTCAAATTAATTTATTATTCTTCATTTATAAAATGATACTACTTATGACTTTGTGGATTCATAGATCTTCATGAAAAGATATATTAGAGTGTTTTTGGAAACATTAAGATGCATAACCTATTTCAACTAAAACTCACTTGGGTTGGCCCGGTGATATTGACTTGAGACCTGAGAGTGTGCTCCTCTTCAAGGTCTCAGGTTCGAATCTCCGTAGTGTCAATTTAGGTGGACTAGTTTAGTTTCTTAAAAAAAATTACCTATTTCAACTATCTTTAGCTAGTCTACACGGTCAAATCGGTGATTAGTCTCATAACCGGTCTAAATTGACGAGGGTGAAAATTATATTGAACTAGCTCTCTAGCAACAAGTATAGCTTATGATATTTCTTTGCACTCAACAGGTGAAGATTCTTAGGAAGGAATCCTACTGGTACAAAGGCACTGGATCAGTTGTTGCTGTTGATCAAGTGAGTGACCTCAAACTCAAATTTCAACCAAACCTTTAAGTTTTTTCCTCATAATCCATTGGTAACTCAGCTCTTGTTTAACGGATTTTTCTTTGTTGTGCAGGACCCCAAGACTCGCTACCCTGTCGTGGTTCGATTCCAGAAAGTGAACTACGCCAATGTATCGACAAATAACTATGCTTTGGATGAGATTGAAGAAGTTGTTTAA

Protein sequence

>MS.gene94806.t1

MASSSMASAATGFLLSLNVAANNTTSSRSTMLMFPLKSNNIGSSSSRLVVRATDEAAPAAPAASTAPAGDAAPTPKPKPPPIGPKRGSKVKILRKESYWYKGTGSVVAVDQDPKTRYPVVVRFQKVNYANVSTNNYALDEIEEVV