Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43447.t1 | XP_024641902.1 | 97.3 | 111 | 3 | 0 | 1 | 111 | 330 | 440 | 2.70E-53 | 218 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43447.t1 | Q53RK8 | 91.9 | 111 | 9 | 0 | 1 | 111 | 397 | 507 | 1.4e-53 | 210.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43447.t1 | G7KQ16 | 97.3 | 111 | 3 | 0 | 1 | 111 | 330 | 440 | 2.0e-53 | 218.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene43447 | 0.836721 | 7.93E-57 | -1.69E-46 |
| MS.gene049159 | MS.gene43447 | 0.833593 | 4.88E-56 | -1.69E-46 |
| MS.gene04918 | MS.gene43447 | 0.800836 | 1.20E-48 | -1.69E-46 |
| MS.gene04920 | MS.gene43447 | 0.816883 | 4.40E-52 | -1.69E-46 |
| MS.gene049254 | MS.gene43447 | 0.826732 | 2.31E-54 | -1.69E-46 |
| MS.gene049283 | MS.gene43447 | 0.845924 | 3.00E-59 | -1.69E-46 |
| MS.gene049368 | MS.gene43447 | 0.835163 | 1.97E-56 | -1.69E-46 |
| MS.gene049404 | MS.gene43447 | 0.80164 | 8.19E-49 | -1.69E-46 |
| MS.gene049452 | MS.gene43447 | 0.83912 | 1.92E-57 | -1.69E-46 |
| MS.gene049499 | MS.gene43447 | 0.840751 | 7.20E-58 | -1.69E-46 |
| MS.gene049801 | MS.gene43447 | 0.801962 | 7.03E-49 | -1.69E-46 |
| MS.gene049983 | MS.gene43447 | 0.855858 | 4.76E-62 | -1.69E-46 |
| MS.gene050085 | MS.gene43447 | 0.801368 | 9.31E-49 | -1.69E-46 |
| MS.gene050105 | MS.gene43447 | 0.807008 | 6.23E-50 | -1.69E-46 |
| MS.gene050273 | MS.gene43447 | 0.806077 | 9.80E-50 | -1.69E-46 |
| MS.gene050414 | MS.gene43447 | 0.815736 | 7.93E-52 | -1.69E-46 |
| MS.gene050646 | MS.gene43447 | 0.820063 | 8.36E-53 | -1.69E-46 |
| MS.gene050654 | MS.gene43447 | 0.835286 | 1.83E-56 | -1.69E-46 |
| MS.gene050689 | MS.gene43447 | 0.807737 | 4.37E-50 | -1.69E-46 |
| MS.gene050759 | MS.gene43447 | 0.813547 | 2.42E-51 | -1.69E-46 |
| MS.gene050941 | MS.gene43447 | 0.805578 | 1.25E-49 | -1.69E-46 |
| MS.gene050962 | MS.gene43447 | 0.802135 | 6.48E-49 | -1.69E-46 |
| MS.gene051066 | MS.gene43447 | 0.829674 | 4.51E-55 | -1.69E-46 |
| MS.gene051198 | MS.gene43447 | 0.847783 | 9.30E-60 | -1.69E-46 |
| MS.gene051279 | MS.gene43447 | 0.825513 | 4.50E-54 | -1.69E-46 |
| MS.gene051343 | MS.gene43447 | 0.804489 | 2.11E-49 | -1.69E-46 |
| MS.gene051363 | MS.gene43447 | 0.814188 | 1.75E-51 | -1.69E-46 |
| MS.gene051368 | MS.gene43447 | 0.826915 | 2.09E-54 | -1.69E-46 |
| MS.gene051597 | MS.gene43447 | 0.818628 | 1.78E-52 | -1.69E-46 |
| MS.gene051628 | MS.gene43447 | 0.802599 | 5.20E-49 | -1.69E-46 |
| MS.gene051744 | MS.gene43447 | 0.803246 | 3.82E-49 | -1.69E-46 |
| MS.gene051800 | MS.gene43447 | 0.806412 | 8.33E-50 | -1.69E-46 |
| MS.gene052037 | MS.gene43447 | 0.811358 | 7.29E-51 | -1.69E-46 |
| MS.gene052307 | MS.gene43447 | 0.800651 | 1.30E-48 | -1.69E-46 |
| MS.gene052342 | MS.gene43447 | 0.808603 | 2.86E-50 | -1.69E-46 |
| MS.gene052463 | MS.gene43447 | 0.824971 | 6.05E-54 | -1.69E-46 |
| MS.gene052516 | MS.gene43447 | 0.819073 | 1.41E-52 | -1.69E-46 |
| MS.gene052518 | MS.gene43447 | 0.804282 | 2.33E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene43447 | 0.817092 | 3.94E-52 | -1.69E-46 |
| MS.gene052534 | MS.gene43447 | 0.805118 | 1.56E-49 | -1.69E-46 |
| MS.gene052582 | MS.gene43447 | 0.807188 | 5.71E-50 | -1.69E-46 |
| MS.gene052584 | MS.gene43447 | 0.805927 | 1.05E-49 | -1.69E-46 |
| MS.gene052677 | MS.gene43447 | 0.800117 | 1.68E-48 | -1.69E-46 |
| MS.gene052954 | MS.gene43447 | 0.822736 | 2.02E-53 | -1.69E-46 |
| MS.gene053008 | MS.gene43447 | 0.817163 | 3.80E-52 | -1.69E-46 |
| MS.gene053058 | MS.gene43447 | 0.801488 | 8.79E-49 | -1.69E-46 |
| MS.gene053079 | MS.gene43447 | 0.847256 | 1.30E-59 | -1.69E-46 |
| MS.gene05308 | MS.gene43447 | 0.81586 | 7.45E-52 | -1.69E-46 |
| MS.gene053226 | MS.gene43447 | 0.817451 | 3.28E-52 | -1.69E-46 |
| MS.gene05364 | MS.gene43447 | 0.821568 | 3.77E-53 | -1.69E-46 |
| MS.gene053931 | MS.gene43447 | 0.833624 | 4.79E-56 | -1.69E-46 |
| MS.gene05415 | MS.gene43447 | 0.802241 | 6.16E-49 | -1.69E-46 |
| MS.gene054236 | MS.gene43447 | 0.803991 | 2.68E-49 | -1.69E-46 |
| MS.gene054479 | MS.gene43447 | 0.833052 | 6.66E-56 | -1.69E-46 |
| MS.gene05458 | MS.gene43447 | 0.815036 | 1.14E-51 | -1.69E-46 |
| MS.gene054641 | MS.gene43447 | 0.800276 | 1.55E-48 | -1.69E-46 |
| MS.gene054651 | MS.gene43447 | 0.833902 | 4.08E-56 | -1.69E-46 |
| MS.gene054657 | MS.gene43447 | 0.812889 | 3.38E-51 | -1.69E-46 |
| MS.gene054893 | MS.gene43447 | 0.800719 | 1.26E-48 | -1.69E-46 |
| MS.gene05517 | MS.gene43447 | 0.81391 | 2.01E-51 | -1.69E-46 |
| MS.gene055222 | MS.gene43447 | 0.805979 | 1.03E-49 | -1.69E-46 |
| MS.gene055228 | MS.gene43447 | 0.811421 | 7.06E-51 | -1.69E-46 |
| MS.gene055513 | MS.gene43447 | 0.826964 | 2.03E-54 | -1.69E-46 |
| MS.gene055818 | MS.gene43447 | 0.80368 | 3.11E-49 | -1.69E-46 |
| MS.gene055841 | MS.gene43447 | 0.844073 | 9.48E-59 | -1.69E-46 |
| MS.gene055926 | MS.gene43447 | 0.813362 | 2.66E-51 | -1.69E-46 |
| MS.gene056045 | MS.gene43447 | 0.830421 | 2.97E-55 | -1.69E-46 |
| MS.gene056174 | MS.gene43447 | 0.820734 | 5.87E-53 | -1.69E-46 |
| MS.gene056308 | MS.gene43447 | 0.813275 | 2.78E-51 | -1.69E-46 |
| MS.gene056366 | MS.gene43447 | 0.804985 | 1.66E-49 | -1.69E-46 |
| MS.gene056776 | MS.gene43447 | 0.81608 | 6.65E-52 | -1.69E-46 |
| MS.gene056831 | MS.gene43447 | 0.812795 | 3.54E-51 | -1.69E-46 |
| MS.gene05686 | MS.gene43447 | 0.837345 | 5.49E-57 | -1.69E-46 |
| MS.gene05690 | MS.gene43447 | 0.8152 | 1.04E-51 | -1.69E-46 |
| MS.gene056973 | MS.gene43447 | 0.814743 | 1.32E-51 | -1.69E-46 |
| MS.gene057034 | MS.gene43447 | 0.805733 | 1.16E-49 | -1.69E-46 |
| MS.gene057208 | MS.gene43447 | 0.876221 | 1.66E-68 | -1.69E-46 |
| MS.gene057371 | MS.gene43447 | 0.817633 | 2.98E-52 | -1.69E-46 |
| MS.gene057444 | MS.gene43447 | 0.806558 | 7.76E-50 | -1.69E-46 |
| MS.gene057598 | MS.gene43447 | 0.813476 | 2.51E-51 | -1.69E-46 |
| MS.gene057855 | MS.gene43447 | 0.80011 | 1.68E-48 | -1.69E-46 |
| MS.gene058008 | MS.gene43447 | 0.826382 | 2.80E-54 | -1.69E-46 |
| MS.gene058009 | MS.gene43447 | 0.840837 | 6.83E-58 | -1.69E-46 |
| MS.gene058010 | MS.gene43447 | 0.854675 | 1.05E-61 | -1.69E-46 |
| MS.gene058054 | MS.gene43447 | 0.801472 | 8.86E-49 | -1.69E-46 |
| MS.gene058126 | MS.gene43447 | 0.812524 | 4.06E-51 | -1.69E-46 |
| MS.gene058159 | MS.gene43447 | 0.80096 | 1.13E-48 | -1.69E-46 |
| MS.gene058292 | MS.gene43447 | 0.82213 | 2.79E-53 | -1.69E-46 |
| MS.gene058427 | MS.gene43447 | 0.806224 | 9.13E-50 | -1.69E-46 |
| MS.gene058548 | MS.gene43447 | 0.816363 | 5.75E-52 | -1.69E-46 |
| MS.gene058595 | MS.gene43447 | 0.820779 | 5.73E-53 | -1.69E-46 |
| MS.gene058781 | MS.gene43447 | 0.869195 | 3.71E-66 | -1.69E-46 |
| MS.gene058783 | MS.gene43447 | 0.860422 | 2.09E-63 | -1.69E-46 |
| MS.gene058979 | MS.gene43447 | 0.845595 | 3.68E-59 | -1.69E-46 |
| MS.gene059146 | MS.gene43447 | 0.803767 | 2.98E-49 | -1.69E-46 |
| MS.gene05952 | MS.gene43447 | 0.80806 | 3.73E-50 | -1.69E-46 |
| MS.gene059842 | MS.gene43447 | 0.823937 | 1.06E-53 | -1.69E-46 |
| MS.gene060011 | MS.gene43447 | 0.841496 | 4.59E-58 | -1.69E-46 |
| MS.gene060244 | MS.gene43447 | 0.812854 | 3.44E-51 | -1.69E-46 |
| MS.gene060310 | MS.gene43447 | 0.82727 | 1.72E-54 | -1.69E-46 |
| MS.gene060365 | MS.gene43447 | 0.813866 | 2.06E-51 | -1.69E-46 |
| MS.gene060435 | MS.gene43447 | 0.803081 | 4.13E-49 | -1.69E-46 |
| MS.gene060527 | MS.gene43447 | 0.800079 | 1.70E-48 | -1.69E-46 |
| MS.gene06052 | MS.gene43447 | 0.828747 | 7.57E-55 | -1.69E-46 |
| MS.gene060932 | MS.gene43447 | 0.809321 | 2.01E-50 | -1.69E-46 |
| MS.gene060933 | MS.gene43447 | 0.839833 | 1.25E-57 | -1.69E-46 |
| MS.gene060935 | MS.gene43447 | 0.872539 | 2.94E-67 | -1.69E-46 |
| MS.gene060937 | MS.gene43447 | 0.869299 | 3.43E-66 | -1.69E-46 |
| MS.gene061100 | MS.gene43447 | 0.821665 | 3.58E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43447.t1 | MTR_6g005320 | 97.297 | 111 | 3 | 0 | 1 | 111 | 330 | 440 | 6.24e-71 | 224 |
| MS.gene43447.t1 | MTR_4g134170 | 97.297 | 111 | 3 | 0 | 1 | 111 | 368 | 478 | 2.43e-70 | 224 |
| MS.gene43447.t1 | MTR_3g467550 | 50.000 | 102 | 50 | 1 | 1 | 101 | 164 | 265 | 1.22e-28 | 109 |
| MS.gene43447.t1 | MTR_3g467550 | 50.000 | 102 | 50 | 1 | 1 | 101 | 195 | 296 | 1.38e-28 | 109 |
| MS.gene43447.t1 | MTR_3g467550 | 50.000 | 102 | 50 | 1 | 1 | 101 | 195 | 296 | 2.15e-28 | 108 |
| MS.gene43447.t1 | MTR_7g109720 | 54.839 | 93 | 42 | 0 | 2 | 94 | 311 | 403 | 1.34e-27 | 107 |
| MS.gene43447.t1 | MTR_6g005550 | 50.505 | 99 | 49 | 0 | 1 | 99 | 242 | 340 | 3.29e-27 | 105 |
| MS.gene43447.t1 | MTR_1g054630 | 49.485 | 97 | 49 | 0 | 1 | 97 | 237 | 333 | 5.41e-27 | 105 |
| MS.gene43447.t1 | MTR_3g083690 | 47.423 | 97 | 51 | 0 | 1 | 97 | 229 | 325 | 1.64e-26 | 103 |
| MS.gene43447.t1 | MTR_4g105100 | 51.546 | 97 | 47 | 0 | 1 | 97 | 280 | 376 | 2.10e-26 | 103 |
| MS.gene43447.t1 | MTR_4g105100 | 51.546 | 97 | 47 | 0 | 1 | 97 | 280 | 376 | 4.10e-26 | 102 |
| MS.gene43447.t1 | MTR_2g007000 | 43.636 | 110 | 54 | 2 | 1 | 107 | 582 | 686 | 1.11e-25 | 101 |
| MS.gene43447.t1 | MTR_2g100230 | 46.729 | 107 | 52 | 1 | 1 | 107 | 174 | 275 | 4.43e-25 | 99.4 |
| MS.gene43447.t1 | MTR_4g114120 | 41.071 | 112 | 63 | 1 | 1 | 112 | 229 | 337 | 4.93e-25 | 99.4 |
| MS.gene43447.t1 | MTR_2g032630 | 45.918 | 98 | 53 | 0 | 1 | 98 | 573 | 670 | 3.41e-23 | 94.4 |
| MS.gene43447.t1 | MTR_3g449250 | 45.455 | 99 | 52 | 1 | 1 | 99 | 169 | 265 | 5.64e-22 | 90.9 |
| MS.gene43447.t1 | MTR_3g449250 | 45.455 | 99 | 52 | 1 | 1 | 99 | 169 | 265 | 7.95e-22 | 90.5 |
| MS.gene43447.t1 | MTR_2g039960 | 41.414 | 99 | 58 | 0 | 1 | 99 | 114 | 212 | 2.78e-19 | 82.8 |
| MS.gene43447.t1 | MTR_5g027840 | 44.681 | 94 | 52 | 0 | 1 | 94 | 183 | 276 | 3.71e-19 | 82.8 |
| MS.gene43447.t1 | MTR_2g099620 | 39.474 | 114 | 59 | 3 | 1 | 105 | 98 | 210 | 9.52e-19 | 81.3 |
| MS.gene43447.t1 | MTR_4g125970 | 38.053 | 113 | 62 | 1 | 1 | 105 | 131 | 243 | 1.05e-18 | 81.3 |
| MS.gene43447.t1 | MTR_4g125970 | 38.053 | 113 | 62 | 1 | 1 | 105 | 114 | 226 | 1.14e-18 | 80.9 |
| MS.gene43447.t1 | MTR_2g099620 | 39.474 | 114 | 59 | 3 | 1 | 105 | 114 | 226 | 1.17e-18 | 80.9 |
| MS.gene43447.t1 | MTR_7g057250 | 36.283 | 113 | 69 | 1 | 1 | 110 | 258 | 370 | 4.21e-18 | 79.7 |
| MS.gene43447.t1 | MTR_5g090120 | 38.384 | 99 | 59 | 1 | 1 | 99 | 160 | 256 | 4.91e-18 | 79.3 |
| MS.gene43447.t1 | MTR_8g018400 | 38.596 | 114 | 60 | 3 | 1 | 105 | 114 | 226 | 6.08e-18 | 79.0 |
| MS.gene43447.t1 | MTR_3g049990 | 38.384 | 99 | 60 | 1 | 1 | 98 | 228 | 326 | 2.79e-17 | 77.4 |
| MS.gene43447.t1 | MTR_6g033070 | 35.052 | 97 | 63 | 0 | 1 | 97 | 220 | 316 | 2.32e-16 | 74.7 |
| MS.gene43447.t1 | MTR_1g095810 | 32.593 | 135 | 77 | 3 | 3 | 124 | 228 | 361 | 1.30e-15 | 72.4 |
| MS.gene43447.t1 | MTR_7g076820 | 30.000 | 100 | 70 | 0 | 1 | 100 | 223 | 322 | 4.51e-15 | 70.9 |
| MS.gene43447.t1 | MTR_6g056080 | 39.362 | 94 | 57 | 0 | 1 | 94 | 186 | 279 | 6.47e-15 | 70.5 |
| MS.gene43447.t1 | MTR_2g438110 | 34.694 | 98 | 64 | 0 | 1 | 98 | 107 | 204 | 6.48e-15 | 70.5 |
| MS.gene43447.t1 | MTR_4g079320 | 41.053 | 95 | 55 | 1 | 1 | 94 | 94 | 188 | 6.98e-15 | 70.5 |
| MS.gene43447.t1 | MTR_7g056830 | 37.374 | 99 | 61 | 1 | 1 | 98 | 85 | 183 | 7.49e-15 | 70.5 |
| MS.gene43447.t1 | MTR_6g082080 | 37.374 | 99 | 61 | 1 | 1 | 98 | 85 | 183 | 7.49e-15 | 70.5 |
| MS.gene43447.t1 | MTR_7g076830 | 29.000 | 100 | 71 | 0 | 1 | 100 | 216 | 315 | 1.16e-14 | 69.7 |
| MS.gene43447.t1 | MTR_7g076800 | 29.000 | 100 | 71 | 0 | 1 | 100 | 214 | 313 | 1.28e-14 | 69.7 |
| MS.gene43447.t1 | MTR_0915s0010 | 29.000 | 100 | 71 | 0 | 1 | 100 | 62 | 161 | 1.93e-14 | 68.6 |
| MS.gene43447.t1 | MTR_1g026830 | 31.852 | 135 | 78 | 3 | 3 | 124 | 230 | 363 | 3.80e-14 | 68.6 |
| MS.gene43447.t1 | MTR_1g102410 | 39.362 | 94 | 57 | 0 | 1 | 94 | 143 | 236 | 7.93e-14 | 67.4 |
| MS.gene43447.t1 | MTR_2g041140 | 36.000 | 100 | 62 | 2 | 1 | 98 | 217 | 316 | 3.29e-13 | 65.9 |
| MS.gene43447.t1 | MTR_4g459350 | 33.663 | 101 | 65 | 2 | 1 | 99 | 121 | 221 | 3.82e-13 | 64.7 |
| MS.gene43447.t1 | MTR_8g027780 | 36.190 | 105 | 58 | 4 | 1 | 98 | 187 | 289 | 5.80e-13 | 65.1 |
| MS.gene43447.t1 | MTR_8g097000 | 34.259 | 108 | 56 | 4 | 1 | 98 | 369 | 471 | 6.35e-13 | 65.1 |
| MS.gene43447.t1 | MTR_4g059460 | 33.663 | 101 | 65 | 2 | 1 | 99 | 124 | 224 | 7.01e-13 | 64.7 |
| MS.gene43447.t1 | MTR_2g043360 | 33.663 | 101 | 65 | 2 | 1 | 99 | 121 | 221 | 8.02e-13 | 64.7 |
| MS.gene43447.t1 | MTR_8g027780 | 36.190 | 105 | 58 | 4 | 1 | 98 | 187 | 289 | 8.26e-13 | 64.7 |
| MS.gene43447.t1 | MTR_6g039840 | 30.189 | 106 | 74 | 0 | 1 | 106 | 16 | 121 | 1.34e-12 | 63.9 |
| MS.gene43447.t1 | MTR_6g039840 | 30.189 | 106 | 74 | 0 | 1 | 106 | 192 | 297 | 1.40e-12 | 63.9 |
| MS.gene43447.t1 | MTR_3g071620 | 35.644 | 101 | 62 | 3 | 1 | 98 | 94 | 194 | 1.71e-12 | 63.5 |
| MS.gene43447.t1 | MTR_8g027770 | 37.143 | 105 | 57 | 4 | 1 | 98 | 282 | 384 | 2.26e-12 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43447.t1 | AT2G33730 | 91.346 | 104 | 9 | 0 | 1 | 104 | 395 | 498 | 9.43e-62 | 201 |
| MS.gene43447.t1 | AT1G28180 | 76.842 | 95 | 22 | 0 | 1 | 95 | 55 | 149 | 4.11e-48 | 158 |
| MS.gene43447.t1 | AT1G28180 | 73.016 | 63 | 17 | 0 | 1 | 63 | 55 | 117 | 2.19e-28 | 101 |
| MS.gene43447.t1 | AT2G47330 | 52.688 | 93 | 44 | 0 | 2 | 94 | 308 | 400 | 4.10e-27 | 105 |
| MS.gene43447.t1 | AT3G58510 | 49.485 | 97 | 49 | 0 | 1 | 97 | 235 | 331 | 4.29e-27 | 105 |
| MS.gene43447.t1 | AT3G58510 | 49.485 | 97 | 49 | 0 | 1 | 97 | 235 | 331 | 4.29e-27 | 105 |
| MS.gene43447.t1 | AT3G58510 | 49.485 | 97 | 49 | 0 | 1 | 97 | 235 | 331 | 4.29e-27 | 105 |
| MS.gene43447.t1 | AT5G63120 | 48.598 | 107 | 50 | 1 | 1 | 107 | 244 | 345 | 2.44e-26 | 103 |
| MS.gene43447.t1 | AT2G42520 | 48.454 | 97 | 50 | 0 | 1 | 97 | 243 | 339 | 2.72e-26 | 103 |
| MS.gene43447.t1 | AT2G42520 | 48.454 | 97 | 50 | 0 | 1 | 97 | 243 | 339 | 2.72e-26 | 103 |
| MS.gene43447.t1 | AT2G42520 | 48.454 | 97 | 50 | 0 | 1 | 97 | 243 | 339 | 2.72e-26 | 103 |
| MS.gene43447.t1 | AT3G58570 | 48.454 | 97 | 50 | 0 | 1 | 97 | 230 | 326 | 9.16e-26 | 101 |
| MS.gene43447.t1 | AT5G63120 | 48.598 | 107 | 50 | 1 | 1 | 107 | 244 | 345 | 1.17e-25 | 100 |
| MS.gene43447.t1 | AT5G63120 | 48.598 | 107 | 50 | 1 | 1 | 107 | 244 | 345 | 1.35e-25 | 100 |
| MS.gene43447.t1 | AT5G63120 | 48.598 | 107 | 50 | 1 | 1 | 107 | 244 | 345 | 1.35e-25 | 100 |
| MS.gene43447.t1 | AT5G63120 | 48.598 | 107 | 50 | 1 | 1 | 107 | 244 | 345 | 1.35e-25 | 100 |
| MS.gene43447.t1 | AT3G01540 | 48.485 | 99 | 51 | 0 | 1 | 99 | 236 | 334 | 2.01e-25 | 100 |
| MS.gene43447.t1 | AT3G01540 | 48.485 | 99 | 51 | 0 | 1 | 99 | 236 | 334 | 2.01e-25 | 100 |
| MS.gene43447.t1 | AT3G01540 | 48.485 | 99 | 51 | 0 | 1 | 99 | 236 | 334 | 2.01e-25 | 100 |
| MS.gene43447.t1 | AT3G01540 | 48.485 | 99 | 51 | 0 | 1 | 99 | 236 | 334 | 2.07e-25 | 100 |
| MS.gene43447.t1 | AT5G14610 | 47.475 | 99 | 52 | 0 | 1 | 99 | 247 | 345 | 3.43e-25 | 99.8 |
| MS.gene43447.t1 | AT5G14610 | 47.475 | 99 | 52 | 0 | 1 | 99 | 239 | 337 | 4.37e-25 | 99.8 |
| MS.gene43447.t1 | AT5G14610 | 47.475 | 99 | 52 | 0 | 1 | 99 | 307 | 405 | 4.39e-25 | 99.8 |
| MS.gene43447.t1 | AT5G14610 | 47.475 | 99 | 52 | 0 | 1 | 99 | 239 | 337 | 4.41e-25 | 99.8 |
| MS.gene43447.t1 | AT5G14610 | 47.475 | 99 | 52 | 0 | 1 | 99 | 247 | 345 | 4.65e-25 | 99.8 |
| MS.gene43447.t1 | AT5G14610 | 47.475 | 99 | 52 | 0 | 1 | 99 | 108 | 206 | 8.23e-25 | 98.6 |
| MS.gene43447.t1 | AT5G14610 | 47.475 | 99 | 52 | 0 | 1 | 99 | 108 | 206 | 8.23e-25 | 98.6 |
| MS.gene43447.t1 | AT1G20920 | 42.727 | 110 | 55 | 2 | 1 | 107 | 270 | 374 | 8.98e-25 | 99.0 |
| MS.gene43447.t1 | AT1G55150 | 46.729 | 107 | 52 | 1 | 1 | 107 | 178 | 279 | 1.07e-24 | 98.2 |
| MS.gene43447.t1 | AT1G20920 | 42.727 | 110 | 55 | 2 | 1 | 107 | 608 | 712 | 1.40e-24 | 98.2 |
| MS.gene43447.t1 | AT1G20920 | 42.727 | 110 | 55 | 2 | 1 | 107 | 608 | 712 | 1.40e-24 | 98.2 |
| MS.gene43447.t1 | AT1G20920 | 42.727 | 110 | 55 | 2 | 1 | 107 | 608 | 712 | 1.40e-24 | 98.2 |
| MS.gene43447.t1 | AT1G20920 | 42.727 | 110 | 55 | 2 | 1 | 107 | 608 | 712 | 1.40e-24 | 98.2 |
| MS.gene43447.t1 | AT1G20920 | 42.727 | 110 | 55 | 2 | 1 | 107 | 608 | 712 | 1.40e-24 | 98.2 |
| MS.gene43447.t1 | AT1G20920 | 42.727 | 110 | 55 | 2 | 1 | 107 | 608 | 712 | 1.40e-24 | 98.2 |
| MS.gene43447.t1 | AT1G55150 | 46.729 | 107 | 52 | 1 | 1 | 107 | 203 | 304 | 1.60e-24 | 97.8 |
| MS.gene43447.t1 | AT3G06480 | 45.918 | 98 | 53 | 0 | 1 | 98 | 513 | 610 | 5.73e-24 | 96.7 |
| MS.gene43447.t1 | AT3G06480 | 45.918 | 98 | 53 | 0 | 1 | 98 | 513 | 610 | 5.86e-24 | 96.7 |
| MS.gene43447.t1 | AT3G09620 | 42.727 | 110 | 55 | 2 | 1 | 107 | 476 | 580 | 1.09e-23 | 95.9 |
| MS.gene43447.t1 | AT3G09620 | 42.727 | 110 | 55 | 2 | 1 | 107 | 475 | 579 | 1.10e-23 | 95.9 |
| MS.gene43447.t1 | AT5G26742 | 42.424 | 99 | 55 | 1 | 1 | 99 | 186 | 282 | 2.42e-20 | 86.3 |
| MS.gene43447.t1 | AT5G26742 | 42.424 | 99 | 55 | 1 | 1 | 99 | 186 | 282 | 2.42e-20 | 86.3 |
| MS.gene43447.t1 | AT5G26742 | 42.424 | 99 | 55 | 1 | 1 | 99 | 93 | 189 | 3.27e-20 | 85.9 |
| MS.gene43447.t1 | AT1G31970 | 47.872 | 94 | 49 | 0 | 1 | 94 | 197 | 290 | 6.77e-20 | 84.7 |
| MS.gene43447.t1 | AT3G13920 | 38.053 | 113 | 62 | 1 | 1 | 105 | 103 | 215 | 1.99e-19 | 83.2 |
| MS.gene43447.t1 | AT3G13920 | 38.053 | 113 | 62 | 1 | 1 | 105 | 108 | 220 | 2.42e-19 | 82.8 |
| MS.gene43447.t1 | AT3G13920 | 38.053 | 113 | 62 | 1 | 1 | 105 | 113 | 225 | 2.42e-19 | 82.8 |
| MS.gene43447.t1 | AT3G13920 | 38.053 | 113 | 62 | 1 | 1 | 105 | 113 | 225 | 2.55e-19 | 82.8 |
| MS.gene43447.t1 | AT3G13920 | 38.053 | 113 | 62 | 1 | 1 | 105 | 117 | 229 | 2.62e-19 | 82.8 |
| MS.gene43447.t1 | AT1G72730 | 40.404 | 99 | 59 | 0 | 1 | 99 | 115 | 213 | 3.26e-19 | 82.8 |
| MS.gene43447.t1 | AT1G54270 | 38.053 | 113 | 62 | 1 | 1 | 105 | 108 | 220 | 6.00e-18 | 79.0 |
| MS.gene43447.t1 | AT1G54270 | 38.053 | 113 | 62 | 1 | 1 | 105 | 113 | 225 | 6.47e-18 | 79.0 |
| MS.gene43447.t1 | AT3G02065 | 39.175 | 97 | 59 | 0 | 1 | 97 | 55 | 151 | 1.19e-17 | 78.2 |
| MS.gene43447.t1 | AT3G16840 | 35.965 | 114 | 70 | 1 | 1 | 111 | 286 | 399 | 1.25e-17 | 78.6 |
| MS.gene43447.t1 | AT3G16840 | 35.965 | 114 | 70 | 1 | 1 | 111 | 285 | 398 | 1.28e-17 | 78.6 |
| MS.gene43447.t1 | AT3G02065 | 39.175 | 97 | 59 | 0 | 1 | 97 | 192 | 288 | 2.47e-17 | 77.4 |
| MS.gene43447.t1 | AT3G02065 | 39.175 | 97 | 59 | 0 | 1 | 97 | 192 | 288 | 2.47e-17 | 77.4 |
| MS.gene43447.t1 | AT3G22310 | 37.374 | 99 | 60 | 1 | 1 | 99 | 196 | 292 | 1.35e-16 | 75.5 |
| MS.gene43447.t1 | AT3G22310 | 37.374 | 99 | 60 | 1 | 1 | 99 | 196 | 292 | 1.35e-16 | 75.5 |
| MS.gene43447.t1 | AT3G22330 | 37.755 | 98 | 59 | 1 | 1 | 98 | 184 | 279 | 1.69e-16 | 75.1 |
| MS.gene43447.t1 | AT4G16630 | 34.483 | 145 | 88 | 2 | 1 | 138 | 244 | 388 | 2.70e-16 | 74.7 |
| MS.gene43447.t1 | AT5G51280 | 37.864 | 103 | 58 | 1 | 3 | 99 | 230 | 332 | 3.79e-16 | 74.3 |
| MS.gene43447.t1 | AT4G33370 | 36.190 | 105 | 61 | 1 | 1 | 99 | 179 | 283 | 9.82e-16 | 72.8 |
| MS.gene43447.t1 | AT4G00660 | 31.000 | 100 | 69 | 0 | 1 | 100 | 205 | 304 | 1.51e-15 | 72.4 |
| MS.gene43447.t1 | AT4G00660 | 31.000 | 100 | 69 | 0 | 1 | 100 | 205 | 304 | 1.51e-15 | 72.4 |
| MS.gene43447.t1 | AT3G61240 | 30.000 | 100 | 70 | 0 | 1 | 100 | 198 | 297 | 1.55e-15 | 72.4 |
| MS.gene43447.t1 | AT3G61240 | 30.000 | 100 | 70 | 0 | 1 | 100 | 198 | 297 | 1.55e-15 | 72.4 |
| MS.gene43447.t1 | AT1G77030 | 37.755 | 98 | 60 | 1 | 1 | 97 | 104 | 201 | 1.99e-15 | 72.0 |
| MS.gene43447.t1 | AT2G45810 | 31.000 | 100 | 69 | 0 | 1 | 100 | 228 | 327 | 9.50e-15 | 70.1 |
| MS.gene43447.t1 | AT5G62190 | 36.000 | 100 | 64 | 0 | 1 | 100 | 180 | 279 | 4.64e-14 | 68.2 |
| MS.gene43447.t1 | AT3G19760 | 33.673 | 98 | 65 | 0 | 1 | 98 | 109 | 206 | 9.11e-14 | 67.4 |
| MS.gene43447.t1 | AT1G51380 | 33.673 | 98 | 65 | 0 | 1 | 98 | 96 | 193 | 2.99e-13 | 65.5 |
| MS.gene43447.t1 | AT1G51380 | 33.673 | 98 | 65 | 0 | 1 | 98 | 96 | 193 | 3.40e-13 | 65.1 |
| MS.gene43447.t1 | AT1G63250 | 35.577 | 104 | 60 | 4 | 1 | 98 | 411 | 513 | 3.47e-13 | 65.9 |
| MS.gene43447.t1 | AT4G09730 | 29.907 | 107 | 75 | 0 | 1 | 107 | 195 | 301 | 4.20e-13 | 65.5 |
| MS.gene43447.t1 | AT1G51380 | 33.673 | 98 | 65 | 0 | 1 | 98 | 96 | 193 | 4.35e-13 | 64.7 |
| MS.gene43447.t1 | AT1G51380 | 33.333 | 99 | 66 | 0 | 1 | 99 | 96 | 194 | 5.61e-13 | 65.1 |
| MS.gene43447.t1 | AT2G07750 | 35.577 | 104 | 60 | 4 | 1 | 98 | 458 | 560 | 6.42e-13 | 65.1 |
| MS.gene43447.t1 | AT3G18600 | 32.673 | 101 | 67 | 1 | 1 | 100 | 167 | 267 | 1.72e-12 | 63.5 |
| MS.gene43447.t1 | AT5G05450 | 36.275 | 102 | 60 | 4 | 1 | 98 | 96 | 196 | 2.20e-12 | 63.5 |
| MS.gene43447.t1 | AT5G65900 | 30.693 | 101 | 69 | 1 | 1 | 100 | 232 | 332 | 2.35e-12 | 63.2 |
| MS.gene43447.t1 | AT1G71370 | 34.653 | 101 | 63 | 3 | 1 | 98 | 96 | 196 | 2.67e-12 | 63.2 |
| MS.gene43447.t1 | AT1G16280 | 33.010 | 103 | 66 | 1 | 1 | 100 | 132 | 234 | 2.90e-12 | 63.2 |
| MS.gene43447.t1 | AT5G60990 | 32.323 | 99 | 66 | 1 | 1 | 98 | 95 | 193 | 3.77e-11 | 59.7 |
Find 40 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGATGAGAAGAGGATTTAT+AGG | 0.256546 | 8.4:-612353 | MS.gene43447:CDS |
| GGTTAAATTTGCTCAGTATA+TGG | 0.266751 | 8.4:-612639 | MS.gene43447:CDS |
| ATTAAAGTTGTTTCCATTGT+TGG | 0.333853 | 8.4:-612614 | MS.gene43447:CDS |
| GTTAAATTTGCTCAGTATAT+GGG | 0.349756 | 8.4:-612638 | MS.gene43447:CDS |
| AAGTTCCTCATCTCATTCTC+TGG | 0.363983 | 8.4:+612374 | None:intergenic |
| GGCTGATCGTATGATTGATA+TGG | 0.372547 | 8.4:-612453 | MS.gene43447:CDS |
| TGGTCGTTTGATTGATTGCT+TGG | 0.389906 | 8.4:-612525 | MS.gene43447:CDS |
| GGGGTTTGAGCCGCAGGTCA+TGG | 0.418088 | 8.4:-612432 | MS.gene43447:CDS |
| CTTAAACCCTTGCTCCTCAA+TGG | 0.420410 | 8.4:+612583 | None:intergenic |
| TCTGGTTTGAGATTACTAGA+AGG | 0.435140 | 8.4:+612392 | None:intergenic |
| TGATATGGGGTTTGAGCCGC+AGG | 0.441038 | 8.4:-612438 | MS.gene43447:CDS |
| GGGTTTGAGCCGCAGGTCAT+GGG | 0.480370 | 8.4:-612431 | MS.gene43447:CDS |
| GCAAGGGTTTAAGATAAGAC+AGG | 0.485716 | 8.4:-612573 | MS.gene43447:CDS |
| GGCTTGCTAGAAAGTATTTG+AGG | 0.491288 | 8.4:-612287 | None:intergenic |
| GCTGATCGTATGATTGATAT+GGG | 0.495582 | 8.4:-612452 | MS.gene43447:CDS |
| CGAGCTTGCTCAGCAGATTG+AGG | 0.503683 | 8.4:-612669 | MS.gene43447:CDS |
| TGCTACCATGCCTCCTGCGG+TGG | 0.509902 | 8.4:-612313 | MS.gene43447:CDS |
| CCATGCCTCCTGCGGTGGAG+AGG | 0.521827 | 8.4:-612308 | MS.gene43447:CDS |
| ATGAGGAACTTGATGAGAAG+AGG | 0.531706 | 8.4:-612362 | MS.gene43447:CDS |
| CTAGCAAGCCTCTCCACCGC+AGG | 0.541300 | 8.4:+612300 | None:intergenic |
| TGCTGAGCAAGCTCGCGAGT+CGG | 0.542852 | 8.4:+612677 | None:intergenic |
| TTAAATTTGCTCAGTATATG+GGG | 0.545312 | 8.4:-612637 | MS.gene43447:CDS |
| AATGGACTGACCACCAACAA+TGG | 0.547761 | 8.4:+612601 | None:intergenic |
| TGTTGGTGGTCAGTCCATTG+AGG | 0.549272 | 8.4:-612597 | MS.gene43447:CDS |
| CCTCTCCACCGCAGGAGGCA+TGG | 0.551441 | 8.4:+612308 | None:intergenic |
| GTCAGTCCATTGAGGAGCAA+GGG | 0.551504 | 8.4:-612589 | MS.gene43447:CDS |
| AAAGTTGTTTCCATTGTTGG+TGG | 0.555528 | 8.4:-612611 | MS.gene43447:CDS |
| GCAAGCCTCTCCACCGCAGG+AGG | 0.558194 | 8.4:+612303 | None:intergenic |
| GAAATTGTGATTGCGACTCC+TGG | 0.569519 | 8.4:-612545 | MS.gene43447:CDS |
| GCCGCAGGTCATGGGTGTGT+TGG | 0.578251 | 8.4:-612423 | MS.gene43447:CDS |
| GGTCAGTCCATTGAGGAGCA+AGG | 0.586693 | 8.4:-612590 | MS.gene43447:CDS |
| TAATTATGTTGTTCTTGATG+AGG | 0.610318 | 8.4:-612474 | MS.gene43447:CDS |
| CAAGGGTTTAAGATAAGACA+GGG | 0.610952 | 8.4:-612572 | MS.gene43447:CDS |
| TTGCTTGGAGCGTCGCTATG+CGG | 0.622750 | 8.4:-612510 | MS.gene43447:CDS |
| TCCAACACACCCATGACCTG+CGG | 0.624778 | 8.4:+612422 | None:intergenic |
| AAGCAATCAATCAAACGACC+AGG | 0.636109 | 8.4:+612527 | None:intergenic |
| CAGTGCTACCATGCCTCCTG+CGG | 0.636466 | 8.4:-612316 | MS.gene43447:CDS |
| TCAGCAGATTGAGGATGAGA+CGG | 0.638997 | 8.4:-612660 | MS.gene43447:CDS |
| TCAAACCAGAGAATGAGATG+AGG | 0.650755 | 8.4:-612379 | MS.gene43447:CDS |
| CTGATCGTATGATTGATATG+GGG | 0.672880 | 8.4:-612451 | MS.gene43447:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TAATTATGTTGTTCTTGATG+AGG | - | chr8.4:612498-612517 | MS.gene43447:CDS | 25.0% |
| !! | ATTAAAGTTGTTTCCATTGT+TGG | - | chr8.4:612358-612377 | MS.gene43447:CDS | 25.0% |
| !! | GTTAAATTTGCTCAGTATAT+GGG | - | chr8.4:612334-612353 | MS.gene43447:CDS | 25.0% |
| !! | TTAAATTTGCTCAGTATATG+GGG | - | chr8.4:612335-612354 | MS.gene43447:CDS | 25.0% |
| TTGATGAGAAGAGGATTTAT+AGG | - | chr8.4:612619-612638 | MS.gene43447:CDS | 30.0% | |
| ! | GGTTAAATTTGCTCAGTATA+TGG | - | chr8.4:612333-612352 | MS.gene43447:CDS | 30.0% |
| CTGATCGTATGATTGATATG+GGG | - | chr8.4:612521-612540 | MS.gene43447:CDS | 35.0% | |
| GCTGATCGTATGATTGATAT+GGG | - | chr8.4:612520-612539 | MS.gene43447:CDS | 35.0% | |
| ! | AAAGTTGTTTCCATTGTTGG+TGG | - | chr8.4:612361-612380 | MS.gene43447:CDS | 35.0% |
| ! | CAAGGGTTTAAGATAAGACA+GGG | - | chr8.4:612400-612419 | MS.gene43447:CDS | 35.0% |
| ! | TCTGGTTTGAGATTACTAGA+AGG | + | chr8.4:612583-612602 | None:intergenic | 35.0% |
| AAGCAATCAATCAAACGACC+AGG | + | chr8.4:612448-612467 | None:intergenic | 40.0% | |
| AAGTTCCTCATCTCATTCTC+TGG | + | chr8.4:612601-612620 | None:intergenic | 40.0% | |
| ATGAGGAACTTGATGAGAAG+AGG | - | chr8.4:612610-612629 | MS.gene43447:CDS | 40.0% | |
| GGCTGATCGTATGATTGATA+TGG | - | chr8.4:612519-612538 | MS.gene43447:CDS | 40.0% | |
| TCAAACCAGAGAATGAGATG+AGG | - | chr8.4:612593-612612 | MS.gene43447:CDS | 40.0% | |
| TGGTCGTTTGATTGATTGCT+TGG | - | chr8.4:612447-612466 | MS.gene43447:CDS | 40.0% | |
| ! | GCAAGGGTTTAAGATAAGAC+AGG | - | chr8.4:612399-612418 | MS.gene43447:CDS | 40.0% |
| CTTAAACCCTTGCTCCTCAA+TGG | + | chr8.4:612392-612411 | None:intergenic | 45.0% | |
| GAAATTGTGATTGCGACTCC+TGG | - | chr8.4:612427-612446 | MS.gene43447:CDS | 45.0% | |
| TCAGCAGATTGAGGATGAGA+CGG | - | chr8.4:612312-612331 | MS.gene43447:CDS | 45.0% | |
| !! | AATGGACTGACCACCAACAA+TGG | + | chr8.4:612374-612393 | None:intergenic | 45.0% |
| GTCAGTCCATTGAGGAGCAA+GGG | - | chr8.4:612383-612402 | MS.gene43447:CDS | 50.0% | |
| !! | TGTTGGTGGTCAGTCCATTG+AGG | - | chr8.4:612375-612394 | MS.gene43447:CDS | 50.0% |
| GGTCAGTCCATTGAGGAGCA+AGG | - | chr8.4:612382-612401 | MS.gene43447:CDS | 55.0% | |
| TCCAACACACCCATGACCTG+CGG | + | chr8.4:612553-612572 | None:intergenic | 55.0% | |
| TGATATGGGGTTTGAGCCGC+AGG | - | chr8.4:612534-612553 | MS.gene43447:CDS | 55.0% | |
| ! | CGAGCTTGCTCAGCAGATTG+AGG | - | chr8.4:612303-612322 | MS.gene43447:CDS | 55.0% |
| !! | TTGCTTGGAGCGTCGCTATG+CGG | - | chr8.4:612462-612481 | MS.gene43447:CDS | 55.0% |
| CAGTGCTACCATGCCTCCTG+CGG | - | chr8.4:612656-612675 | MS.gene43447:CDS | 60.0% | |
| GGGTTTGAGCCGCAGGTCAT+GGG | - | chr8.4:612541-612560 | MS.gene43447:CDS | 60.0% | |
| TGCTGAGCAAGCTCGCGAGT+CGG | + | chr8.4:612298-612317 | None:intergenic | 60.0% | |
| CTAGCAAGCCTCTCCACCGC+AGG | + | chr8.4:612675-612694 | None:intergenic | 65.0% | |
| GCCGCAGGTCATGGGTGTGT+TGG | - | chr8.4:612549-612568 | MS.gene43447:CDS | 65.0% | |
| GGGGTTTGAGCCGCAGGTCA+TGG | - | chr8.4:612540-612559 | MS.gene43447:CDS | 65.0% | |
| TGCTACCATGCCTCCTGCGG+TGG | - | chr8.4:612659-612678 | MS.gene43447:CDS | 65.0% | |
| CCATGCCTCCTGCGGTGGAG+AGG | - | chr8.4:612664-612683 | MS.gene43447:CDS | 70.0% | |
| CCTCTCCACCGCAGGAGGCA+TGG | + | chr8.4:612667-612686 | None:intergenic | 70.0% | |
| GCAAGCCTCTCCACCGCAGG+AGG | + | chr8.4:612672-612691 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 612289 | 612705 | 612289 | ID=MS.gene43447 |
| chr8.4 | mRNA | 612289 | 612705 | 612289 | ID=MS.gene43447.t1;Parent=MS.gene43447 |
| chr8.4 | exon | 612289 | 612705 | 612289 | ID=MS.gene43447.t1.exon1;Parent=MS.gene43447.t1 |
| chr8.4 | CDS | 612289 | 612705 | 612289 | ID=cds.MS.gene43447.t1;Parent=MS.gene43447.t1 |
| Gene Sequence |
| Protein sequence |
