AlfalfaGEDB | MS.gene59860

M. sativa cultivar XinJiangDaYe / MS.gene59860

KEGG: -
GO: GO:0006950 GO:0006952 GO:0008150 GO:0050896
PFAM: PF00407.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59860.t1	XP_013445246.1	93.6	156	10	0	1	156	1	156	3.00E-80	307.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59860.t1	Q941R6	35.3	153	95	4	3	152	20	171	7.7e-21	101.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59860.t1	I3SJ62	93.6	156	10	0	1	156	1	156	2.1e-80	307.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049711	MS.gene59860	0.80762	4.63E-50	-1.69E-46
MS.gene049807	MS.gene59860	0.825818	3.81E-54	-1.69E-46
MS.gene050559	MS.gene59860	0.807258	5.52E-50	-1.69E-46
MS.gene050562	MS.gene59860	0.802318	5.94E-49	-1.69E-46
MS.gene052533	MS.gene59860	0.811061	8.45E-51	-1.69E-46
MS.gene054490	MS.gene59860	0.813975	1.95E-51	-1.69E-46
MS.gene055141	MS.gene59860	0.803558	3.29E-49	-1.69E-46
MS.gene056677	MS.gene59860	0.820908	5.35E-53	-1.69E-46
MS.gene058492	MS.gene59860	0.80534	1.40E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene59862	MS.gene59860	PPI
MS.gene016336	MS.gene59860	PPI
MS.gene59860	MS.gene016317	PPI
MS.gene74358	MS.gene59860	PPI
MS.gene74344	MS.gene59860	PPI
MS.gene99548	MS.gene59860	PPI
MS.gene066940	MS.gene59860	PPI
MS.gene016315	MS.gene59860	PPI
MS.gene016326	MS.gene59860	PPI
MS.gene59860	MS.gene74341	PPI
MS.gene016337	MS.gene59860	PPI
MS.gene83129	MS.gene59860	PPI
MS.gene59860	MS.gene77781	PPI
MS.gene59140	MS.gene59860	PPI
MS.gene74353	MS.gene59860	PPI
MS.gene77771	MS.gene59860	PPI
MS.gene59869	MS.gene59860	PPI
MS.gene74354	MS.gene59860	PPI
MS.gene016314	MS.gene59860	PPI
MS.gene016330	MS.gene59860	PPI
MS.gene77758	MS.gene59860	PPI
MS.gene026605	MS.gene59860	PPI
MS.gene058383	MS.gene59860	PPI
MS.gene59860	MS.gene74346	PPI
MS.gene74341	MS.gene59860	PPI
MS.gene59860	MS.gene74367	PPI
MS.gene058382	MS.gene59860	PPI
MS.gene77780	MS.gene59860	PPI
MS.gene59860	MS.gene59847	PPI
MS.gene62988	MS.gene59860	PPI
MS.gene74365	MS.gene59860	PPI
MS.gene59860	MS.gene59859	PPI
MS.gene77768	MS.gene59860	PPI
MS.gene74346	MS.gene59860	PPI
MS.gene59863	MS.gene59860	PPI
MS.gene59841	MS.gene59860	PPI
MS.gene77770	MS.gene59860	PPI
MS.gene74342	MS.gene59860	PPI
MS.gene016307	MS.gene59860	PPI
MS.gene59860	MS.gene016307	PPI
MS.gene59860	MS.gene59840	PPI
MS.gene59860	MS.gene058386	PPI
MS.gene058384	MS.gene59860	PPI
MS.gene74355	MS.gene59860	PPI
MS.gene058386	MS.gene59860	PPI
MS.gene74343	MS.gene59860	PPI
MS.gene77774	MS.gene59860	PPI
MS.gene77761	MS.gene59860	PPI
MS.gene77763	MS.gene59860	PPI
MS.gene77769	MS.gene59860	PPI
MS.gene59857	MS.gene59860	PPI
MS.gene74364	MS.gene59860	PPI
MS.gene016327	MS.gene59860	PPI
MS.gene74352	MS.gene59860	PPI
MS.gene74367	MS.gene59860	PPI
MS.gene59849	MS.gene59860	PPI
MS.gene59860	MS.gene016315	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59860.t1	MTR_8g045695	93.590	156	10	0	1	156	1	156	2.51e-106	300
MS.gene59860.t1	MTR_8g045735	85.065	154	23	0	1	154	1	154	5.55e-98	279
MS.gene59860.t1	MTR_8g045665	83.974	156	25	0	1	156	1	156	7.07e-96	274
MS.gene59860.t1	MTR_8g045640	82.051	156	28	0	1	156	1	156	1.76e-92	265
MS.gene59860.t1	MTR_8g045560	80.769	156	30	0	1	156	1	156	3.26e-91	262
MS.gene59860.t1	MTR_8g045520	76.433	157	36	1	1	156	1	157	2.48e-85	247
MS.gene59860.t1	MTR_8g045400	75.159	157	37	2	1	156	1	156	5.58e-85	246
MS.gene59860.t1	MTR_8g045490	72.611	157	41	2	1	156	1	156	2.06e-81	237
MS.gene59860.t1	MTR_8g045555	70.395	152	44	1	1	151	1	152	7.79e-77	225
MS.gene59860.t1	MTR_8g045570	69.079	152	46	1	1	151	1	152	5.92e-76	223
MS.gene59860.t1	MTR_8g045300	66.667	156	51	1	1	155	1	156	7.88e-76	223
MS.gene59860.t1	MTR_1g030810	45.098	153	80	3	1	150	1	152	2.41e-40	133
MS.gene59860.t1	MTR_1g030820	39.869	153	88	3	1	150	1	152	1.54e-37	126
MS.gene59860.t1	MTR_1g030840	40.385	156	84	5	1	148	1	155	1.04e-30	108
MS.gene59860.t1	MTR_8g012550	32.667	150	97	4	6	153	8	155	4.61e-25	94.4
MS.gene59860.t1	MTR_1g031640	38.514	148	81	6	7	151	7	147	5.45e-23	89.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59860.t1	AT1G70840	35.294	153	95	4	3	152	20	171	1.90e-25	95.9
MS.gene59860.t1	AT5G28010	35.294	153	95	4	3	152	15	166	2.08e-25	95.5
MS.gene59860.t1	AT5G28010	35.294	153	95	4	3	152	15	166	2.08e-25	95.5
MS.gene59860.t1	AT1G70830	36.601	153	93	4	3	152	22	173	3.85e-25	95.1
MS.gene59860.t1	AT1G70830	36.364	154	94	4	3	153	22	174	5.88e-25	95.5
MS.gene59860.t1	AT1G70890	35.948	153	94	4	3	152	7	158	1.33e-24	93.2
MS.gene59860.t1	AT1G70830	36.364	154	94	4	3	153	22	174	4.26e-24	94.4
MS.gene59860.t1	AT1G70830	36.364	154	94	4	3	153	22	174	7.52e-24	95.1
MS.gene59860.t1	AT1G70830	35.294	153	95	4	3	152	184	335	2.42e-22	91.3
MS.gene59860.t1	AT1G70830	35.294	153	95	4	3	152	137	288	7.36e-23	91.7
MS.gene59860.t1	AT5G28000	32.026	153	100	4	3	152	13	164	5.78e-22	86.7
MS.gene59860.t1	AT4G23670	34.641	153	96	4	1	151	1	151	8.91e-22	85.9
MS.gene59860.t1	AT1G70850	34.000	150	95	4	3	149	9	157	2.31e-21	87.0
MS.gene59860.t1	AT1G14930	31.210	157	104	4	1	155	1	155	3.46e-21	84.3
MS.gene59860.t1	AT1G70850	34.000	150	95	4	3	149	9	157	5.54e-21	87.4
MS.gene59860.t1	AT1G70850	35.664	143	88	4	13	152	175	316	9.08e-21	86.7
MS.gene59860.t1	AT1G70850	34.000	150	95	4	3	149	9	157	5.54e-21	87.4
MS.gene59860.t1	AT1G70850	35.664	143	88	4	13	152	175	316	9.08e-21	86.7
MS.gene59860.t1	AT2G01520	31.373	153	101	4	1	151	1	151	1.36e-20	82.8
MS.gene59860.t1	AT2G01530	32.680	153	99	4	1	151	1	151	1.35e-19	80.1
MS.gene59860.t1	AT1G14940	29.299	157	107	4	1	155	1	155	1.39e-18	77.8
MS.gene59860.t1	AT1G70870	35.484	155	78	5	1	150	1	138	2.64e-18	76.6
MS.gene59860.t1	AT1G14950	29.936	157	106	4	1	155	1	155	9.03e-18	75.5
MS.gene59860.t1	AT4G14060	30.065	153	103	4	1	151	1	151	1.03e-17	75.5
MS.gene59860.t1	AT1G23130	28.188	149	104	3	3	149	10	157	1.98e-17	74.7
MS.gene59860.t1	AT4G23680	30.263	152	102	4	1	150	1	150	5.11e-17	73.6
MS.gene59860.t1	AT1G70880	28.758	153	105	4	3	152	8	159	8.31e-17	73.2
MS.gene59860.t1	AT1G35260	29.861	144	96	4	9	149	8	149	1.10e-16	72.8
MS.gene59860.t1	AT1G30990	29.801	151	101	5	1	149	1	148	8.49e-16	70.5
MS.gene59860.t1	AT1G23120	27.333	150	101	5	3	149	1	145	1.52e-15	69.7
MS.gene59860.t1	AT1G23120	26.241	141	98	4	10	149	60	195	3.80e-15	69.7
MS.gene59860.t1	AT1G14960	28.387	155	107	4	1	153	1	153	7.32e-15	68.2
MS.gene59860.t1	AT1G24020	30.968	155	98	4	1	150	1	151	5.07e-14	65.9
MS.gene59860.t1	AT1G24020	30.968	155	98	4	1	150	1	151	5.07e-14	65.9
MS.gene59860.t1	AT3G26460	26.797	153	109	3	1	151	1	152	1.31e-13	64.7
MS.gene59860.t1	AT1G35310	28.058	139	96	4	13	149	12	148	2.09e-13	63.9
MS.gene59860.t1	AT3G26450	25.325	154	110	5	1	151	1	152	1.10e-11	59.7
MS.gene59860.t1	AT1G14940	26.115	157	96	4	1	155	1	139	1.39e-11	58.9

Find 29 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCACTTACAAGCTATT+TGG	0.220413	8.3:+53714124	MS.gene59860:CDS
GTCTTTAAGCTCATCTTAGA+AGG	0.291250	8.3:+53714169	MS.gene59860:CDS
ATCTTCACCTTCATGAAGTT+TGG	0.299460	8.3:-53712589	None:intergenic
AACATTAATGAAAGAGTTCA+TGG	0.350725	8.3:+53712564	MS.gene59860:CDS
ATGGAAGTTCTGCTGTTAGA+TGG	0.407851	8.3:+53714206	MS.gene59860:CDS
GGTAAACTTATTACTGAACT+TGG	0.415223	8.3:+53712480	MS.gene59860:CDS
TGGAGATATTGAAAACTATA+AGG	0.439418	8.3:+53714147	MS.gene59860:CDS
AGGAAATCCATCCATCCATT+TGG	0.440781	8.3:-53714268	None:intergenic
AAACACTGGACTTATGTCAT+AGG	0.470664	8.3:+53712633	MS.gene59860:CDS
ATCACTTACAAGCTATTTGG+TGG	0.474679	8.3:+53714127	MS.gene59860:CDS
AAAAGCACTAGAGATATTGA+TGG	0.477335	8.3:+53714295	MS.gene59860:CDS
AACTTCATGAAGGTGAAGAT+TGG	0.494334	8.3:+53712592	MS.gene59860:CDS
TCATCTTGTCAAAGGGGAAA+AGG	0.504175	8.3:+53714318	MS.gene59860:CDS
AGATTGGCATCATACTGATT+CGG	0.504369	8.3:+53712608	MS.gene59860:CDS
TTGATGGTCATCTTGTCAAA+GGG	0.506740	8.3:+53714311	MS.gene59860:CDS
GAGGATATCGATACTCCAAA+TGG	0.515854	8.3:+53714253	MS.gene59860:CDS
CGATACTCCAAATGGATGGA+TGG	0.534738	8.3:+53714261	MS.gene59860:CDS
GTAAACTTATTACTGAACTT+GGG	0.542207	8.3:+53712481	MS.gene59860:CDS
ATTGATGGTCATCTTGTCAA+AGG	0.543582	8.3:+53714310	MS.gene59860:CDS
TCTTTAAGCTCATCTTAGAA+GGG	0.545692	8.3:+53714170	MS.gene59860:CDS
AGTTTGTAGAACTTGTCAGC+TGG	0.569465	8.3:-53712513	None:intergenic
GAAGGGCTTAACAAGGCTGA+TGG	0.584478	8.3:+53714187	MS.gene59860:CDS
ATATCGATACTCCAAATGGA+TGG	0.593139	8.3:+53714257	MS.gene59860:CDS
ATACTGATTCGGTTAAACAC+TGG	0.606046	8.3:+53712619	MS.gene59860:CDS
CATCTTAGAAGGGCTTAACA+AGG	0.611102	8.3:+53714180	MS.gene59860:CDS
CATGGTGCCAAACTTCATGA+AGG	0.632424	8.3:+53712582	MS.gene59860:CDS
TGATGGTCATCTTGTCAAAG+GGG	0.652198	8.3:+53714312	MS.gene59860:CDS
TGACGTAATAACAGATGGCG+AGG	0.668621	8.3:+53714054	MS.gene59860:intron
TGATTATGAGAAGAACAATG+AGG	0.681814	8.3:+53714234	MS.gene59860:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CTTAATTATCAAAAAAATAA+TGG	-	chr8.3:53713029-53713048	None:intergenic	10.0%
!!!	ATTTTTAAAAAAATGTGAAA+GGG	+	chr8.3:53713684-53713703	MS.gene59860:intron	10.0%
!!	AAATTCTAAATTATGTCAAT+AGG	-	chr8.3:53712861-53712880	None:intergenic	15.0%
!!	AAATTTACTATGAAATTGTA+GGG	+	chr8.3:53713355-53713374	MS.gene59860:intron	15.0%
!!	GAATAATATATATTATGAGT+GGG	-	chr8.3:53713066-53713085	None:intergenic	15.0%
!!	TAAATTTACTATGAAATTGT+AGG	+	chr8.3:53713354-53713373	MS.gene59860:intron	15.0%
!!	TATAGTAATAAAAAGAAGAT+TGG	+	chr8.3:53712747-53712766	MS.gene59860:intron	15.0%
!!	TGAATAATATATATTATGAG+TGG	-	chr8.3:53713067-53713086	None:intergenic	15.0%
!!!	ATCTTTAAATAGTATTTTAG+AGG	+	chr8.3:53713776-53713795	MS.gene59860:intron	15.0%
!!!	CATTTTTAAAAAAATGTGAA+AGG	+	chr8.3:53713683-53713702	MS.gene59860:intron	15.0%
!!!	GGATTTATATTTTGTTTTTT+AGG	+	chr8.3:53713596-53713615	MS.gene59860:intron	15.0%
!!!	GTTTTCTATTACAAATTTTT+TGG	+	chr8.3:53712716-53712735	MS.gene59860:intron	15.0%
!!!	CAATAAAATACGTTTTTCAA+AGG	-	chr8.3:53714014-53714033	None:intergenic	20.0%
!!!	CCTTAAAGTTTTTAGTAAAA+CGG	+	chr8.3:53713286-53713305	MS.gene59860:intron	20.0%
!!!	GTATAATTTTTTCTTTACGA+TGG	+	chr8.3:53713180-53713199	MS.gene59860:intron	20.0%
!!!	TATGTATTTGATTTATGCTT+AGG	+	chr8.3:53713385-53713404	MS.gene59860:intron	20.0%
!!!	TTTTGTTTTTTAGGATACAA+AGG	+	chr8.3:53713605-53713624	MS.gene59860:intron	20.0%
!	AAAAAATTATACTCGTACCA+AGG	-	chr8.3:53713172-53713191	None:intergenic	25.0%
!	AAAATCACTTACAAGCTATT+TGG	+	chr8.3:53714124-53714143	MS.gene59860:CDS	25.0%
!	AACAATGCATAATATATGCA+AGG	+	chr8.3:53713840-53713859	MS.gene59860:intron	25.0%
!	AACATTAATGAAAGAGTTCA+TGG	+	chr8.3:53712564-53712583	MS.gene59860:CDS	25.0%
!	AGTATCATTTGTTTCCTTTA+AGG	-	chr8.3:53713131-53713150	None:intergenic	25.0%
!	GTAAACTTATTACTGAACTT+GGG	+	chr8.3:53712481-53712500	MS.gene59860:CDS	25.0%
!	GTTTGTATAATATGATCCTT+CGG	+	chr8.3:53713245-53713264	MS.gene59860:intron	25.0%
!	TGGAGATATTGAAAACTATA+AGG	+	chr8.3:53714147-53714166	MS.gene59860:CDS	25.0%
!	TTATCTCTAGACAAAAAATC+TGG	-	chr8.3:53713217-53713236	None:intergenic	25.0%
!	TTGTCTAGAGATAAAAAGTA+GGG	+	chr8.3:53713223-53713242	MS.gene59860:intron	25.0%
!	TTTGTCTAGAGATAAAAAGT+AGG	+	chr8.3:53713222-53713241	MS.gene59860:intron	25.0%
!!	CCGTTTTACTAAAAACTTTA+AGG	-	chr8.3:53713289-53713308	None:intergenic	25.0%
!!	TCATTTTACAACAGTAATGT+GGG	+	chr8.3:53713575-53713594	MS.gene59860:intron	25.0%
!!!	GTGCTTTATTTAAAGATCTA+AGG	-	chr8.3:53713721-53713740	None:intergenic	25.0%
	AAAGCAACAAATGACCTTAA+AGG	+	chr8.3:53713114-53713133	MS.gene59860:intron	30.0%
	AAATGATACTCAACCATCTT+TGG	+	chr8.3:53713141-53713160	MS.gene59860:intron	30.0%
	AGCCATGAAATTACAAATCA+TGG	-	chr8.3:53713461-53713480	None:intergenic	30.0%
	GGTAAACTTATTACTGAACT+TGG	+	chr8.3:53712480-53712499	MS.gene59860:CDS	30.0%
	TAATTTCATGGCTCTTTCTT+GGG	+	chr8.3:53713468-53713487	MS.gene59860:intron	30.0%
	TCTTCGTTATAGATCTCATT+TGG	-	chr8.3:53712787-53712806	None:intergenic	30.0%
	TCTTTAAGCTCATCTTAGAA+GGG	+	chr8.3:53714170-53714189	MS.gene59860:CDS	30.0%
	TGATTATGAGAAGAACAATG+AGG	+	chr8.3:53714234-53714253	MS.gene59860:CDS	30.0%
!	TGCCATGATTTGTAATTTCA+TGG	+	chr8.3:53713456-53713475	MS.gene59860:intron	30.0%
!!	AAAAGCACTAGAGATATTGA+TGG	+	chr8.3:53714295-53714314	MS.gene59860:CDS	30.0%
!!	GTCATTTTACAACAGTAATG+TGG	+	chr8.3:53713574-53713593	MS.gene59860:intron	30.0%
	AAACACTGGACTTATGTCAT+AGG	+	chr8.3:53712633-53712652	MS.gene59860:CDS	35.0%
	AACTTCATGAAGGTGAAGAT+TGG	+	chr8.3:53712592-53712611	MS.gene59860:CDS	35.0%
	ATATCGATACTCCAAATGGA+TGG	+	chr8.3:53714257-53714276	MS.gene59860:CDS	35.0%
	ATCACTTACAAGCTATTTGG+TGG	+	chr8.3:53714127-53714146	MS.gene59860:CDS	35.0%
	ATCTTCACCTTCATGAAGTT+TGG	-	chr8.3:53712592-53712611	None:intergenic	35.0%
	GCATAATATATGCAAGGTTC+AGG	+	chr8.3:53713846-53713865	MS.gene59860:intron	35.0%
	GTAATTTCATGGCTCTTTCT+TGG	+	chr8.3:53713467-53713486	MS.gene59860:intron	35.0%
	GTCTTTAAGCTCATCTTAGA+AGG	+	chr8.3:53714169-53714188	MS.gene59860:CDS	35.0%
	TATTGAAATCATTGCACACG+AGG	+	chr8.3:53712964-53712983	MS.gene59860:intron	35.0%
	TGATTTATGCTTAGGTGTTC+AGG	+	chr8.3:53713393-53713412	MS.gene59860:intron	35.0%
!	AGATTGGCATCATACTGATT+CGG	+	chr8.3:53712608-53712627	MS.gene59860:CDS	35.0%
!	ATTGATGGTCATCTTGTCAA+AGG	+	chr8.3:53714310-53714329	MS.gene59860:CDS	35.0%
!	TATCTCTAGTGCTTTTGCTA+AGG	-	chr8.3:53714291-53714310	None:intergenic	35.0%
!	TTGATGGTCATCTTGTCAAA+GGG	+	chr8.3:53714311-53714330	MS.gene59860:CDS	35.0%
!!	ATACTGATTCGGTTAAACAC+TGG	+	chr8.3:53712619-53712638	MS.gene59860:CDS	35.0%
!!!	GAATGCCTCTTTCTTTTTTG+TGG	-	chr8.3:53713887-53713906	None:intergenic	35.0%
!!!	TCTTTCTTTTTTGTGGTGGT+CGG	-	chr8.3:53713880-53713899	None:intergenic	35.0%
	AACCATCTTTGGATTCACCT+TGG	+	chr8.3:53713152-53713171	MS.gene59860:intron	40.0%
	AGGAAATCCATCCATCCATT+TGG	-	chr8.3:53714271-53714290	None:intergenic	40.0%
	AGTTTGTAGAACTTGTCAGC+TGG	-	chr8.3:53712516-53712535	None:intergenic	40.0%
	ATGGAAGTTCTGCTGTTAGA+TGG	+	chr8.3:53714206-53714225	MS.gene59860:CDS	40.0%
	CATCTTAGAAGGGCTTAACA+AGG	+	chr8.3:53714180-53714199	MS.gene59860:CDS	40.0%
	CGAGACTGATCTAACAATGA+AGG	+	chr8.3:53712824-53712843	MS.gene59860:intron	40.0%
	GACCACCACAAAAAAGAAAG+AGG	+	chr8.3:53713879-53713898	MS.gene59860:intron	40.0%
	GAGGATATCGATACTCCAAA+TGG	+	chr8.3:53714253-53714272	MS.gene59860:CDS	40.0%
	TACCAAGGTGAATCCAAAGA+TGG	-	chr8.3:53713157-53713176	None:intergenic	40.0%
	TCATCTTGTCAAAGGGGAAA+AGG	+	chr8.3:53714318-53714337	MS.gene59860:CDS	40.0%
	TGATGGTCATCTTGTCAAAG+GGG	+	chr8.3:53714312-53714331	MS.gene59860:CDS	40.0%
!	CGTCTTTAGACATTTTCCGA+AGG	-	chr8.3:53713264-53713283	None:intergenic	40.0%
!!!	CTTTCTTTTTTGTGGTGGTC+GGG	-	chr8.3:53713879-53713898	None:intergenic	40.0%
!!!	TGCCTCTTTCTTTTTTGTGG+TGG	-	chr8.3:53713884-53713903	None:intergenic	40.0%
	CGATACTCCAAATGGATGGA+TGG	+	chr8.3:53714261-53714280	MS.gene59860:CDS	45.0%
	CTTGGGTGCAATGTATGCTT+GGG	+	chr8.3:53713485-53713504	MS.gene59860:intron	45.0%
	GCCATCTGTTATTACGTCAG+AGG	-	chr8.3:53714053-53714072	None:intergenic	45.0%
	GCCTCTGACGTAATAACAGA+TGG	+	chr8.3:53714049-53714068	MS.gene59860:intron	45.0%
	TCTTGGGTGCAATGTATGCT+TGG	+	chr8.3:53713484-53713503	MS.gene59860:intron	45.0%
	TGACGTAATAACAGATGGCG+AGG	+	chr8.3:53714054-53714073	MS.gene59860:intron	45.0%
!	CATGGTGCCAAACTTCATGA+AGG	+	chr8.3:53712582-53712601	MS.gene59860:CDS	45.0%
!!	TATATATTATTCAAAAAAAA+AGG	+	chr8.3:53713074-53713093	MS.gene59860:intron	5.0%
!!	TTATAATAAGTTAATAATAA+AGG	-	chr8.3:53713955-53713974	None:intergenic	5.0%
!!!	CTATTATTATTTTATTTTTT+TGG	-	chr8.3:53713543-53713562	None:intergenic	5.0%
!!!	TAACTTATTATAATATTTTT+TGG	+	chr8.3:53713962-53713981	MS.gene59860:intron	5.0%
!!!	TAATTTAGAATTTTTTTAAT+TGG	+	chr8.3:53712868-53712887	MS.gene59860:intron	5.0%
!!!	TATTATATATTTTTTTAATG+CGG	+	chr8.3:53713918-53713937	MS.gene59860:intron	5.0%
	CTTCGTGTGCTTAGCTCAGT+TGG	+	chr8.3:53713813-53713832	MS.gene59860:intron	50.0%
	GAAGGGCTTAACAAGGCTGA+TGG	+	chr8.3:53714187-53714206	MS.gene59860:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	53712468	53714351	53712468	ID=MS.gene59860
chr8.3	mRNA	53712468	53714351	53712468	ID=MS.gene59860.t1;Parent=MS.gene59860
chr8.3	exon	53712468	53712654	53712468	ID=MS.gene59860.t1.exon1;Parent=MS.gene59860.t1
chr8.3	CDS	53712468	53712654	53712468	ID=cds.MS.gene59860.t1;Parent=MS.gene59860.t1
chr8.3	exon	53714068	53714351	53714068	ID=MS.gene59860.t1.exon2;Parent=MS.gene59860.t1
chr8.3	CDS	53714068	53714351	53714068	ID=cds.MS.gene59860.t1;Parent=MS.gene59860.t1

Gene Sequence

>MS.gene59860

ATGGTGCTTGCTGGTAAACTTATTACTGAACTTGGGATCAAAACACCAGCTGACAAGTTCTACAAACTATTTGCATCAGAACTTCACGAAGTGCAAAACATTAATGAAAGAGTTCATGGTGCCAAACTTCATGAAGGTGAAGATTGGCATCATACTGATTCGGTTAAACACTGGACTTATGTCATAGGTAAATATTGTGTTTCTAATGTTTCGATAACTAAATCATCATCTTTGTTTGATTTGAAAAAGTTTTCTATTACAAATTTTTTGGATGATAATTATAGTAATAAAAAGAAGATTGGTGAGTTAAAGAATACCAAATGAGATCTATAACGAAGAATAAAGATTTGATTCATCGAGACTGATCTAACAATGAAGGCAATATTTCAGCCTATTGACATAATTTAGAATTTTTTTAATTGGTTAGAAAGCTTTTAAGAGTTTTAGATATATAAATTGTTAAAATAAAAAGTTAAATTCATGAAAAAAATTCAGATATTGAAATCATTGCACACGAGGTGAAGTACTTGATCTCTAAAAATAGTACTTAAACTTTATCCATTATTTTTTTGATAATTAAGAGTGAGCTTGAACACCCACTCATAATATATATTATTCAAAAAAAAAGGAAGAGAAAAAAAAAAAAAAAGCAACAAATGACCTTAAAGGAAACAAATGATACTCAACCATCTTTGGATTCACCTTGGTACGAGTATAATTTTTTCTTTACGATGGTGACTACATTTCCAGATTTTTTGTCTAGAGATAAAAAGTAGGGTTTGTATAATATGATCCTTCGGAAAATGTCTAAAGACGAACCTTAAAGTTTTTAGTAAAACGGATATTGAATGTTTTTTTACGAGAAAAACATATTAAACATGTAATTTAAATTTACTATGAAATTGTAGGGTTCATACTATGTATTTGATTTATGCTTAGGTGTTCAGGTGTATGCTTGACTCAAAATTTTCTCTGCTAATACTAATAGTGCCATGATTTGTAATTTCATGGCTCTTTCTTGGGTGCAATGTATGCTTGGGTTTGAGTAGTTTTTATATATTTTTGCATATTTCCAAAAAAATAAAATAATAATAGTGACATGAATTGTCATTTTACAACAGTAATGTGGGATTTATATTTTGTTTTTTAGGATACAAAGGCAAGCTCATAACACTAAGTTAAATCTTGAGCACAAAAAAAAAAAATACATAAATTCATTTTTAAAAAAATGTGAAAGGGAAATAAAGCATCCTTAGATCTTTAAATAAAGCACAAAATTATAAATTTTCATAAAAATCAGCATGTAAGATCTTTAAATAGTATTTTAGAGGCATTTGTTAGATGTCTTCGTGTGCTTAGCTCAGTTGGTATGAACAATGCATAATATATGCAAGGTTCAGGTTCAAATCCCGACCACCACAAAAAAGAAAGAGGCATTCGTTAGATAGTGTATTATATATTTTTTTAATGCGGAAATAAAGCATCCTTTATTATTAACTTATTATAATATTTTTTGGTGCAACTAATCTTTATTATATTAATTCCTTTGAAAAACGTATTTTATTGTTATTATTATTAATTGCCTCTGACGTAATAACAGATGGCGAGGTACACACATGCAATGAGAGTGTTGAAGAAGTTGATGATCAGAACAAAAAAATCACTTACAAGCTATTTGGTGGAGATATTGAAAACTATAAGGTCTTTAAGCTCATCTTAGAAGGGCTTAACAAGGCTGATGGAAGTTCTGCTGTTAGATGGACTATTGATTATGAGAAGAACAATGAGGATATCGATACTCCAAATGGATGGATGGATTTCCTTAGCAAAAGCACTAGAGATATTGATGGTCATCTTGTCAAAGGGGAAAAGGTAGCTCTATAA

Protein sequence

>MS.gene59860.t1

MVLAGKLITELGIKTPADKFYKLFASELHEVQNINERVHGAKLHEGEDWHHTDSVKHWTYVIDGEVHTCNESVEEVDDQNKKITYKLFGGDIENYKVFKLILEGLNKADGSSAVRWTIDYEKNNEDIDTPNGWMDFLSKSTRDIDGHLVKGEKVAL