Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066941.t1 | XP_013445245.1 | 95.2 | 62 | 3 | 0 | 1 | 62 | 1 | 62 | 7.50E-27 | 129 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066941.t1 | A0A072TPY0 | 95.2 | 62 | 3 | 0 | 1 | 62 | 1 | 62 | 5.4e-27 | 129.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene066941 | 0.833575 | 4.93E-56 | -1.69E-46 |
| MS.gene049151 | MS.gene066941 | 0.846187 | 2.54E-59 | -1.69E-46 |
| MS.gene049153 | MS.gene066941 | 0.815515 | 8.89E-52 | -1.69E-46 |
| MS.gene049233 | MS.gene066941 | 0.800536 | 1.38E-48 | -1.69E-46 |
| MS.gene049260 | MS.gene066941 | 0.829513 | 4.93E-55 | -1.69E-46 |
| MS.gene049297 | MS.gene066941 | 0.822178 | 2.72E-53 | -1.69E-46 |
| MS.gene04933 | MS.gene066941 | 0.816097 | 6.59E-52 | -1.69E-46 |
| MS.gene049346 | MS.gene066941 | 0.812944 | 3.29E-51 | -1.69E-46 |
| MS.gene049378 | MS.gene066941 | 0.831735 | 1.41E-55 | -1.69E-46 |
| MS.gene049758 | MS.gene066941 | 0.868343 | 7.01E-66 | -1.69E-46 |
| MS.gene049910 | MS.gene066941 | 0.805463 | 1.32E-49 | -1.69E-46 |
| MS.gene049915 | MS.gene066941 | 0.812613 | 3.88E-51 | -1.69E-46 |
| MS.gene050226 | MS.gene066941 | 0.843936 | 1.03E-58 | -1.69E-46 |
| MS.gene050227 | MS.gene066941 | 0.833589 | 4.89E-56 | -1.69E-46 |
| MS.gene050228 | MS.gene066941 | 0.834373 | 3.11E-56 | -1.69E-46 |
| MS.gene050301 | MS.gene066941 | 0.821186 | 4.61E-53 | -1.69E-46 |
| MS.gene050330 | MS.gene066941 | -0.811053 | 8.49E-51 | -1.69E-46 |
| MS.gene050558 | MS.gene066941 | 0.815105 | 1.10E-51 | -1.69E-46 |
| MS.gene050586 | MS.gene066941 | 0.80936 | 1.97E-50 | -1.69E-46 |
| MS.gene050623 | MS.gene066941 | -0.824508 | 7.77E-54 | -1.69E-46 |
| MS.gene050642 | MS.gene066941 | 0.818071 | 2.37E-52 | -1.69E-46 |
| MS.gene050724 | MS.gene066941 | 0.801537 | 8.59E-49 | -1.69E-46 |
| MS.gene050762 | MS.gene066941 | 0.825695 | 4.07E-54 | -1.69E-46 |
| MS.gene050763 | MS.gene066941 | 0.829101 | 6.21E-55 | -1.69E-46 |
| MS.gene050987 | MS.gene066941 | 0.805761 | 1.14E-49 | -1.69E-46 |
| MS.gene05119 | MS.gene066941 | -0.841763 | 3.90E-58 | -1.69E-46 |
| MS.gene051662 | MS.gene066941 | 0.811072 | 8.41E-51 | -1.69E-46 |
| MS.gene051663 | MS.gene066941 | 0.838756 | 2.38E-57 | -1.69E-46 |
| MS.gene051860 | MS.gene066941 | 0.838842 | 2.26E-57 | -1.69E-46 |
| MS.gene051983 | MS.gene066941 | 0.818582 | 1.82E-52 | -1.69E-46 |
| MS.gene052077 | MS.gene066941 | 0.813746 | 2.19E-51 | -1.69E-46 |
| MS.gene052176 | MS.gene066941 | 0.837858 | 4.06E-57 | -1.69E-46 |
| MS.gene052188 | MS.gene066941 | 0.811593 | 6.48E-51 | -1.69E-46 |
| MS.gene052207 | MS.gene066941 | 0.807723 | 4.40E-50 | -1.69E-46 |
| MS.gene052255 | MS.gene066941 | 0.811146 | 8.10E-51 | -1.69E-46 |
| MS.gene052302 | MS.gene066941 | 0.821763 | 3.40E-53 | -1.69E-46 |
| MS.gene052309 | MS.gene066941 | -0.808753 | 2.65E-50 | -1.69E-46 |
| MS.gene052340 | MS.gene066941 | 0.819186 | 1.33E-52 | -1.69E-46 |
| MS.gene052376 | MS.gene066941 | 0.827317 | 1.67E-54 | -1.69E-46 |
| MS.gene052856 | MS.gene066941 | 0.80675 | 7.07E-50 | -1.69E-46 |
| MS.gene053184 | MS.gene066941 | 0.810461 | 1.14E-50 | -1.69E-46 |
| MS.gene053630 | MS.gene066941 | 0.802451 | 5.58E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene066941 | 0.884848 | 1.35E-71 | -1.69E-46 |
| MS.gene054184 | MS.gene066941 | 0.825403 | 4.78E-54 | -1.69E-46 |
| MS.gene054185 | MS.gene066941 | 0.827131 | 1.85E-54 | -1.69E-46 |
| MS.gene054379 | MS.gene066941 | 0.830798 | 2.40E-55 | -1.69E-46 |
| MS.gene054473 | MS.gene066941 | 0.837853 | 4.07E-57 | -1.69E-46 |
| MS.gene054542 | MS.gene066941 | 0.836056 | 1.17E-56 | -1.69E-46 |
| MS.gene054930 | MS.gene066941 | 0.889307 | 2.72E-73 | -1.69E-46 |
| MS.gene054942 | MS.gene066941 | 0.818268 | 2.14E-52 | -1.69E-46 |
| MS.gene054983 | MS.gene066941 | 0.800067 | 1.71E-48 | -1.69E-46 |
| MS.gene055133 | MS.gene066941 | 0.817616 | 3.01E-52 | -1.69E-46 |
| MS.gene055358 | MS.gene066941 | 0.80953 | 1.81E-50 | -1.69E-46 |
| MS.gene055422 | MS.gene066941 | 0.833482 | 5.20E-56 | -1.69E-46 |
| MS.gene055428 | MS.gene066941 | 0.821314 | 4.31E-53 | -1.69E-46 |
| MS.gene055604 | MS.gene066941 | 0.815617 | 8.43E-52 | -1.69E-46 |
| MS.gene055712 | MS.gene066941 | 0.805124 | 1.55E-49 | -1.69E-46 |
| MS.gene056069 | MS.gene066941 | -0.837191 | 6.01E-57 | -1.69E-46 |
| MS.gene056875 | MS.gene066941 | 0.825971 | 3.50E-54 | -1.69E-46 |
| MS.gene056979 | MS.gene066941 | 0.819402 | 1.18E-52 | -1.69E-46 |
| MS.gene057121 | MS.gene066941 | 0.812957 | 3.26E-51 | -1.69E-46 |
| MS.gene057260 | MS.gene066941 | 0.829975 | 3.81E-55 | -1.69E-46 |
| MS.gene057439 | MS.gene066941 | 0.821227 | 4.52E-53 | -1.69E-46 |
| MS.gene057448 | MS.gene066941 | 0.80812 | 3.62E-50 | -1.69E-46 |
| MS.gene057450 | MS.gene066941 | 0.81653 | 5.27E-52 | -1.69E-46 |
| MS.gene057469 | MS.gene066941 | 0.834021 | 3.81E-56 | -1.69E-46 |
| MS.gene057750 | MS.gene066941 | 0.834563 | 2.79E-56 | -1.69E-46 |
| MS.gene057754 | MS.gene066941 | 0.856618 | 2.85E-62 | -1.69E-46 |
| MS.gene058002 | MS.gene066941 | 0.829864 | 4.06E-55 | -1.69E-46 |
| MS.gene05811 | MS.gene066941 | 0.82228 | 2.58E-53 | -1.69E-46 |
| MS.gene058142 | MS.gene066941 | 0.818003 | 2.46E-52 | -1.69E-46 |
| MS.gene058182 | MS.gene066941 | 0.82345 | 1.38E-53 | -1.69E-46 |
| MS.gene058236 | MS.gene066941 | 0.800525 | 1.38E-48 | -1.69E-46 |
| MS.gene058399 | MS.gene066941 | 0.814016 | 1.91E-51 | -1.69E-46 |
| MS.gene058710 | MS.gene066941 | 0.847426 | 1.17E-59 | -1.69E-46 |
| MS.gene058749 | MS.gene066941 | 0.849408 | 3.30E-60 | -1.69E-46 |
| MS.gene058833 | MS.gene066941 | 0.823045 | 1.71E-53 | -1.69E-46 |
| MS.gene05907 | MS.gene066941 | 0.800093 | 1.69E-48 | -1.69E-46 |
| MS.gene059124 | MS.gene066941 | 0.827373 | 1.62E-54 | -1.69E-46 |
| MS.gene059128 | MS.gene066941 | 0.810853 | 9.37E-51 | -1.69E-46 |
| MS.gene059285 | MS.gene066941 | 0.834085 | 3.68E-56 | -1.69E-46 |
| MS.gene059518 | MS.gene066941 | 0.827682 | 1.37E-54 | -1.69E-46 |
| MS.gene05984 | MS.gene066941 | 0.843869 | 1.07E-58 | -1.69E-46 |
| MS.gene059894 | MS.gene066941 | 0.831009 | 2.13E-55 | -1.69E-46 |
| MS.gene059899 | MS.gene066941 | 0.823266 | 1.52E-53 | -1.69E-46 |
| MS.gene060084 | MS.gene066941 | 0.813637 | 2.31E-51 | -1.69E-46 |
| MS.gene06037 | MS.gene066941 | 0.825526 | 4.47E-54 | -1.69E-46 |
| MS.gene060404 | MS.gene066941 | 0.806886 | 6.62E-50 | -1.69E-46 |
| MS.gene060476 | MS.gene066941 | 0.837192 | 6.01E-57 | -1.69E-46 |
| MS.gene060480 | MS.gene066941 | 0.802997 | 4.30E-49 | -1.69E-46 |
| MS.gene060495 | MS.gene066941 | 0.802879 | 4.55E-49 | -1.69E-46 |
| MS.gene060683 | MS.gene066941 | 0.820653 | 6.12E-53 | -1.69E-46 |
| MS.gene060685 | MS.gene066941 | 0.808621 | 2.83E-50 | -1.69E-46 |
| MS.gene060998 | MS.gene066941 | 0.817967 | 2.50E-52 | -1.69E-46 |
| MS.gene061049 | MS.gene066941 | 0.851419 | 8.98E-61 | -1.69E-46 |
| MS.gene061071 | MS.gene066941 | 0.805179 | 1.51E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066941.t1 | MTR_8g045665 | 95.161 | 62 | 3 | 0 | 1 | 62 | 1 | 62 | 3.89e-38 | 123 |
| MS.gene066941.t1 | MTR_8g045695 | 87.097 | 62 | 8 | 0 | 1 | 62 | 1 | 62 | 6.84e-35 | 115 |
| MS.gene066941.t1 | MTR_8g045640 | 87.097 | 62 | 8 | 0 | 1 | 62 | 1 | 62 | 9.39e-35 | 115 |
| MS.gene066941.t1 | MTR_8g045560 | 83.871 | 62 | 10 | 0 | 1 | 62 | 1 | 62 | 3.93e-33 | 110 |
| MS.gene066941.t1 | MTR_8g045520 | 82.258 | 62 | 11 | 0 | 1 | 62 | 1 | 62 | 1.50e-32 | 109 |
| MS.gene066941.t1 | MTR_8g045735 | 79.032 | 62 | 13 | 0 | 1 | 62 | 1 | 62 | 8.03e-32 | 107 |
| MS.gene066941.t1 | MTR_8g045400 | 80.645 | 62 | 12 | 0 | 1 | 62 | 1 | 62 | 9.30e-31 | 105 |
| MS.gene066941.t1 | MTR_8g045490 | 74.194 | 62 | 16 | 0 | 1 | 62 | 1 | 62 | 6.76e-30 | 102 |
| MS.gene066941.t1 | MTR_8g045570 | 69.355 | 62 | 19 | 0 | 1 | 62 | 1 | 62 | 6.57e-27 | 95.1 |
| MS.gene066941.t1 | MTR_8g045300 | 65.574 | 61 | 21 | 0 | 1 | 61 | 1 | 61 | 3.68e-25 | 90.9 |
| MS.gene066941.t1 | MTR_8g045555 | 62.903 | 62 | 23 | 0 | 1 | 62 | 1 | 62 | 4.97e-24 | 87.8 |
| MS.gene066941.t1 | MTR_1g030810 | 43.548 | 62 | 34 | 1 | 1 | 62 | 1 | 61 | 3.42e-12 | 57.4 |
| MS.gene066941.t1 | MTR_1g030820 | 37.097 | 62 | 38 | 1 | 1 | 62 | 1 | 61 | 3.68e-12 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACAAACTTTGTGCACTTCG+TGG | 0.405990 | 8.4:-55340149 | None:intergenic |
| ATCTTCACCTTCATGAAGCT+TGG | 0.441586 | 8.4:-55340187 | None:intergenic |
| GGGAAACTTATCACTGAACT+TGG | 0.445682 | 8.4:+55340078 | MS.gene066941:CDS |
| AAACACTGGACTTATGTCAT+AGG | 0.470664 | 8.4:+55340231 | MS.gene066941:CDS |
| AGGTGAAGATTGGCATCAAA+AGG | 0.482747 | 8.4:+55340200 | MS.gene066941:CDS |
| TTTCCAAATATGGTGCTCGC+TGG | 0.484997 | 8.4:+55340057 | None:intergenic |
| AGCTTCATGAAGGTGAAGAT+TGG | 0.494334 | 8.4:+55340190 | MS.gene066941:CDS |
| TTCCAAATATGGTGCTCGCT+GGG | 0.503897 | 8.4:+55340058 | None:intergenic |
| AGATTGGCATCAAAAGGATT+CGG | 0.511486 | 8.4:+55340206 | MS.gene066941:CDS |
| GAACTTGGGATCAAATCACC+TGG | 0.553911 | 8.4:+55340093 | MS.gene066941:CDS |
| AACTTGGGATCAAATCACCT+GGG | 0.581574 | 8.4:+55340094 | MS.gene066941:CDS |
| CATCATGCCAAGCTTCATGA+AGG | 0.583004 | 8.4:+55340180 | MS.gene066941:CDS |
| AAAAGGATTCGGTTAAACAC+TGG | 0.591334 | 8.4:+55340217 | MS.gene066941:CDS |
| AGTTTGAAGAACTTCTCCCC+AGG | 0.610513 | 8.4:-55340111 | None:intergenic |
| GGAAACTTATCACTGAACTT+GGG | 0.624297 | 8.4:+55340079 | MS.gene066941:CDS |
| ACTTGGGATCAAATCACCTG+GGG | 0.705917 | 8.4:+55340095 | MS.gene066941:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGATTGGCATCAAAAGGATT+CGG | + | chr8.4:55340206-55340225 | MS.gene066941:CDS | 35.0% | |
| GGAAACTTATCACTGAACTT+GGG | + | chr8.4:55340079-55340098 | MS.gene066941:CDS | 35.0% | |
| ! | AAAAGGATTCGGTTAAACAC+TGG | + | chr8.4:55340217-55340236 | MS.gene066941:CDS | 35.0% |
| AACTTGGGATCAAATCACCT+GGG | + | chr8.4:55340094-55340113 | MS.gene066941:CDS | 40.0% | |
| AGCTTCATGAAGGTGAAGAT+TGG | + | chr8.4:55340190-55340209 | MS.gene066941:CDS | 40.0% | |
| AGGTGAAGATTGGCATCAAA+AGG | + | chr8.4:55340200-55340219 | MS.gene066941:CDS | 40.0% | |
| ATCTTCACCTTCATGAAGCT+TGG | - | chr8.4:55340190-55340209 | None:intergenic | 40.0% | |
| GGGAAACTTATCACTGAACT+TGG | + | chr8.4:55340078-55340097 | MS.gene066941:CDS | 40.0% | |
| ACTTGGGATCAAATCACCTG+GGG | + | chr8.4:55340095-55340114 | MS.gene066941:CDS | 45.0% | |
| AGTTTGAAGAACTTCTCCCC+AGG | - | chr8.4:55340114-55340133 | None:intergenic | 45.0% | |
| CACAAACTTTGTGCACTTCG+TGG | - | chr8.4:55340152-55340171 | None:intergenic | 45.0% | |
| CATCATGCCAAGCTTCATGA+AGG | + | chr8.4:55340180-55340199 | MS.gene066941:CDS | 45.0% | |
| GAACTTGGGATCAAATCACC+TGG | + | chr8.4:55340093-55340112 | MS.gene066941:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 55340066 | 55340252 | 55340066 | ID=MS.gene066941 |
| chr8.4 | mRNA | 55340066 | 55340252 | 55340066 | ID=MS.gene066941.t1;Parent=MS.gene066941 |
| chr8.4 | exon | 55340066 | 55340252 | 55340066 | ID=MS.gene066941.t1.exon1;Parent=MS.gene066941.t1 |
| chr8.4 | CDS | 55340066 | 55340252 | 55340066 | ID=cds.MS.gene066941.t1;Parent=MS.gene066941.t1 |
| Gene Sequence |
| Protein sequence |
