AlfalfaGEDB | MS.gene070164

M. sativa cultivar XinJiangDaYe / MS.gene070164

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070164.t1	XP_013470035.1	95.8	72	3	0	1	72	219	290	1.50E-31	144.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070164.t1	Q66PF4	52.8	72	29	3	1	72	218	284	2.1e-13	75.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070164.t1	A0A072VRK6	95.8	72	3	0	1	72	219	290	1.1e-31	144.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049351	MS.gene070164	0.813432	2.57E-51	-1.69E-46
MS.gene049356	MS.gene070164	0.854685	1.04E-61	-1.69E-46
MS.gene049369	MS.gene070164	0.822928	1.82E-53	-1.69E-46
MS.gene049491	MS.gene070164	0.800796	1.22E-48	-1.69E-46
MS.gene049681	MS.gene070164	0.827395	1.60E-54	-1.69E-46
MS.gene049758	MS.gene070164	0.816853	4.46E-52	-1.69E-46
MS.gene049907	MS.gene070164	0.82836	9.39E-55	-1.69E-46
MS.gene050122	MS.gene070164	0.809966	1.46E-50	-1.69E-46
MS.gene050128	MS.gene070164	0.821044	4.98E-53	-1.69E-46
MS.gene050301	MS.gene070164	0.842458	2.55E-58	-1.69E-46
MS.gene050397	MS.gene070164	0.806002	1.02E-49	-1.69E-46
MS.gene050670	MS.gene070164	0.836234	1.05E-56	-1.69E-46
MS.gene050971	MS.gene070164	0.82616	3.16E-54	-1.69E-46
MS.gene05101	MS.gene070164	0.804934	1.70E-49	-1.69E-46
MS.gene05105	MS.gene070164	0.827409	1.59E-54	-1.69E-46
MS.gene051255	MS.gene070164	0.847448	1.15E-59	-1.69E-46
MS.gene051360	MS.gene070164	0.861783	8.05E-64	-1.69E-46
MS.gene051525	MS.gene070164	0.810373	1.19E-50	-1.69E-46
MS.gene051860	MS.gene070164	0.836248	1.05E-56	-1.69E-46
MS.gene052011	MS.gene070164	-0.811707	6.12E-51	-1.69E-46
MS.gene052077	MS.gene070164	0.800476	1.42E-48	-1.69E-46
MS.gene052188	MS.gene070164	0.865265	6.70E-65	-1.69E-46
MS.gene052253	MS.gene070164	0.838332	3.06E-57	-1.69E-46
MS.gene052310	MS.gene070164	0.812114	4.99E-51	-1.69E-46
MS.gene052340	MS.gene070164	0.842646	2.28E-58	-1.69E-46
MS.gene052485	MS.gene070164	0.850857	1.29E-60	-1.69E-46
MS.gene053169	MS.gene070164	0.829909	3.95E-55	-1.69E-46
MS.gene05316	MS.gene070164	0.842145	3.09E-58	-1.69E-46
MS.gene053209	MS.gene070164	0.817776	2.77E-52	-1.69E-46
MS.gene053449	MS.gene070164	0.810331	1.22E-50	-1.69E-46
MS.gene053758	MS.gene070164	0.815778	7.76E-52	-1.69E-46
MS.gene053813	MS.gene070164	0.851536	8.33E-61	-1.69E-46
MS.gene053836	MS.gene070164	0.823435	1.39E-53	-1.69E-46
MS.gene053928	MS.gene070164	0.819057	1.42E-52	-1.69E-46
MS.gene054010	MS.gene070164	0.845148	4.87E-59	-1.69E-46
MS.gene054011	MS.gene070164	0.853627	2.11E-61	-1.69E-46
MS.gene054012	MS.gene070164	0.846426	2.19E-59	-1.69E-46
MS.gene054013	MS.gene070164	0.848357	6.45E-60	-1.69E-46
MS.gene054123	MS.gene070164	0.813365	2.66E-51	-1.69E-46
MS.gene054183	MS.gene070164	0.806262	8.96E-50	-1.69E-46
MS.gene054349	MS.gene070164	0.843696	1.20E-58	-1.69E-46
MS.gene054556	MS.gene070164	0.853417	2.42E-61	-1.69E-46
MS.gene055116	MS.gene070164	0.841618	4.26E-58	-1.69E-46
MS.gene055277	MS.gene070164	0.82784	1.25E-54	-1.69E-46
MS.gene055422	MS.gene070164	0.817945	2.53E-52	-1.69E-46
MS.gene055428	MS.gene070164	0.802602	5.19E-49	-1.69E-46
MS.gene055604	MS.gene070164	0.844222	8.64E-59	-1.69E-46
MS.gene055666	MS.gene070164	0.848833	4.76E-60	-1.69E-46
MS.gene055909	MS.gene070164	0.809378	1.95E-50	-1.69E-46
MS.gene055911	MS.gene070164	0.816614	5.05E-52	-1.69E-46
MS.gene056191	MS.gene070164	0.855282	7.00E-62	-1.69E-46
MS.gene056407	MS.gene070164	0.827633	1.40E-54	-1.69E-46
MS.gene056410	MS.gene070164	0.824463	7.96E-54	-1.69E-46
MS.gene056511	MS.gene070164	0.825982	3.48E-54	-1.69E-46
MS.gene056659	MS.gene070164	0.811592	6.48E-51	-1.69E-46
MS.gene056902	MS.gene070164	0.848552	5.70E-60	-1.69E-46
MS.gene05702	MS.gene070164	0.816936	4.28E-52	-1.69E-46
MS.gene057330	MS.gene070164	0.805886	1.08E-49	-1.69E-46
MS.gene057448	MS.gene070164	0.839351	1.67E-57	-1.69E-46
MS.gene057449	MS.gene070164	0.8383	3.12E-57	-1.69E-46
MS.gene057450	MS.gene070164	0.841667	4.14E-58	-1.69E-46
MS.gene057469	MS.gene070164	0.835657	1.48E-56	-1.69E-46
MS.gene057749	MS.gene070164	0.858603	7.36E-63	-1.69E-46
MS.gene057796	MS.gene070164	0.810768	9.78E-51	-1.69E-46
MS.gene057823	MS.gene070164	0.861491	9.88E-64	-1.69E-46
MS.gene057826	MS.gene070164	0.814576	1.44E-51	-1.69E-46
MS.gene058002	MS.gene070164	0.842237	2.92E-58	-1.69E-46
MS.gene058007	MS.gene070164	0.812208	4.76E-51	-1.69E-46
MS.gene05811	MS.gene070164	0.864651	1.04E-64	-1.69E-46
MS.gene058365	MS.gene070164	0.813937	1.99E-51	-1.69E-46
MS.gene058660	MS.gene070164	0.81664	4.98E-52	-1.69E-46
MS.gene058700	MS.gene070164	0.80463	1.97E-49	-1.69E-46
MS.gene05886	MS.gene070164	0.820352	7.18E-53	-1.69E-46
MS.gene058928	MS.gene070164	0.819662	1.03E-52	-1.69E-46
MS.gene058948	MS.gene070164	0.801033	1.09E-48	-1.69E-46
MS.gene058949	MS.gene070164	0.847256	1.30E-59	-1.69E-46
MS.gene058995	MS.gene070164	0.833904	4.08E-56	-1.69E-46
MS.gene05907	MS.gene070164	0.875257	3.55E-68	-1.69E-46
MS.gene059294	MS.gene070164	0.843561	1.30E-58	-1.69E-46
MS.gene059366	MS.gene070164	0.836234	1.05E-56	-1.69E-46
MS.gene059420	MS.gene070164	0.842203	2.98E-58	-1.69E-46
MS.gene059518	MS.gene070164	0.802047	6.75E-49	-1.69E-46
MS.gene059597	MS.gene070164	0.837209	5.95E-57	-1.69E-46
MS.gene059674	MS.gene070164	0.848916	4.52E-60	-1.69E-46
MS.gene059676	MS.gene070164	0.84648	2.12E-59	-1.69E-46
MS.gene059688	MS.gene070164	0.838491	2.79E-57	-1.69E-46
MS.gene059692	MS.gene070164	0.837771	4.27E-57	-1.69E-46
MS.gene059695	MS.gene070164	0.836568	8.67E-57	-1.69E-46
MS.gene059711	MS.gene070164	0.855212	7.34E-62	-1.69E-46
MS.gene059807	MS.gene070164	0.838956	2.11E-57	-1.69E-46
MS.gene05984	MS.gene070164	0.838947	2.12E-57	-1.69E-46
MS.gene059876	MS.gene070164	0.814531	1.47E-51	-1.69E-46
MS.gene059974	MS.gene070164	0.846894	1.63E-59	-1.69E-46
MS.gene060005	MS.gene070164	0.811182	7.96E-51	-1.69E-46
MS.gene06037	MS.gene070164	0.844676	6.52E-59	-1.69E-46
MS.gene060403	MS.gene070164	0.812779	3.57E-51	-1.69E-46
MS.gene060495	MS.gene070164	0.817896	2.60E-52	-1.69E-46
MS.gene060569	MS.gene070164	0.822127	2.80E-53	-1.69E-46
MS.gene060837	MS.gene070164	0.848762	4.99E-60	-1.69E-46
MS.gene060986	MS.gene070164	0.81805	2.40E-52	-1.69E-46
MS.gene061049	MS.gene070164	0.839485	1.54E-57	-1.69E-46
MS.gene061051	MS.gene070164	-0.802928	4.45E-49	-1.69E-46
MS.gene061154	MS.gene070164	0.821558	3.79E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 10 sgRNAs with CRISPR-Local

Find 14 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTGTAGTTTACGTCTCTTT+TGG	0.299710	1.2:+79902150	MS.gene070164:CDS
CGAAGGACCAACAGTTCTTA+TGG	0.342677	1.2:-79902022	None:intergenic
TTTATCATCATCATTGCTCT+TGG	0.430813	1.2:-79902094	None:intergenic
ACGTAAACTACAGAACCTTT+TGG	0.440135	1.2:-79902141	None:intergenic
AGTGGATACCTATGAAGAAC+TGG	0.455383	1.2:+79901960	None:intergenic
AAAGGAGCAAGCAACATTCA+TGG	0.490192	1.2:+79902063	MS.gene070164:CDS
ATCATGCTCCAGTTCTTCAT+AGG	0.507272	1.2:-79901968	None:intergenic
TCAATCACCATAAGAACTGT+TGG	0.548504	1.2:+79902015	MS.gene070164:CDS
TGGCTAGACACAAAGCCAAA+AGG	0.554363	1.2:+79902126	MS.gene070164:CDS
ATGATGATAAAATTATAGAG+TGG	0.618296	1.2:+79902106	MS.gene070164:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATGATGATAAAATTATAGAG+TGG	+	chr1.2:79902106-79902125	MS.gene070164:CDS	20.0%
!!	TTTAAAAATCCAAATGCAAA+AGG	+	chr1.2:79902045-79902064	MS.gene070164:CDS	20.0%
	TTTATCATCATCATTGCTCT+TGG	-	chr1.2:79902097-79902116	None:intergenic	30.0%
!	GCATTTGGATTTTTAAACGA+AGG	-	chr1.2:79902042-79902061	None:intergenic	30.0%
	TCAATCACCATAAGAACTGT+TGG	+	chr1.2:79902015-79902034	MS.gene070164:CDS	35.0%
!	ACGTAAACTACAGAACCTTT+TGG	-	chr1.2:79902144-79902163	None:intergenic	35.0%
!	CTGTAGTTTACGTCTCTTTT+GGG	+	chr1.2:79902151-79902170	MS.gene070164:CDS	35.0%
!	TCTGTAGTTTACGTCTCTTT+TGG	+	chr1.2:79902150-79902169	MS.gene070164:CDS	35.0%
	AAAGGAGCAAGCAACATTCA+TGG	+	chr1.2:79902063-79902082	MS.gene070164:CDS	40.0%
	ATCATGCTCCAGTTCTTCAT+AGG	-	chr1.2:79901971-79901990	None:intergenic	40.0%
!	TTGCTTGCTCCTTTTGCATT+TGG	-	chr1.2:79902057-79902076	None:intergenic	40.0%
	CGAAGGACCAACAGTTCTTA+TGG	-	chr1.2:79902025-79902044	None:intergenic	45.0%
	TGGCTAGACACAAAGCCAAA+AGG	+	chr1.2:79902126-79902145	MS.gene070164:CDS	45.0%
!!!	ACGTCTCTTTTGGGACTCTA+TGG	+	chr1.2:79902160-79902179	MS.gene070164:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	79901962	79902179	79901962	ID=MS.gene070164
chr1.2	mRNA	79901962	79902179	79901962	ID=MS.gene070164.t1;Parent=MS.gene070164
chr1.2	exon	79901962	79902179	79901962	ID=MS.gene070164.t1.exon1;Parent=MS.gene070164.t1
chr1.2	CDS	79901962	79902179	79901962	ID=cds.MS.gene070164.t1;Parent=MS.gene070164.t1

Gene Sequence	>MS.gene070164 TGGATACCTATGAAGAACTGGAGCATGATTTCATAGATTATATTTCGAAAAAATCAATCACCATAAGAACTGTTGGTCCTTCGTTTAAAAATCCAAATGCAAAAGGAGCAAGCAACATTCATGGTGATTTCGCCAAGAGCAATGATGATGATAAAATTATAGAGTGGCTAGACACAAAGCCAAAAGGTTCTGTAGTTTACGTCTCTTTTGGGACTCTA
Protein sequence	>MS.gene070164.t1 WIPMKNWSMISUIIFRKNQSPUELLVLRLKIQMQKEQATFMVISPRAMMMIKLUSGUTQSQKVLUFTSLLGL