AlfalfaGEDB | MS.gene070950

M. sativa cultivar XinJiangDaYe / MS.gene070950

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070950.t1	RHN81973.1	98.6	72	1	0	1	72	1	72	2.40E-32	147.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070950.t1	B7FF93	98.6	72	1	0	1	72	1	72	1.7e-32	147.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049356	MS.gene070950	0.86928	3.49E-66	-1.69E-46
MS.gene049369	MS.gene070950	0.860343	2.21E-63	-1.69E-46
MS.gene049396	MS.gene070950	0.828008	1.14E-54	-1.69E-46
MS.gene049488	MS.gene070950	0.800152	1.65E-48	-1.69E-46
MS.gene049661	MS.gene070950	0.808991	2.36E-50	-1.69E-46
MS.gene049662	MS.gene070950	0.806745	7.08E-50	-1.69E-46
MS.gene049758	MS.gene070950	0.800262	1.57E-48	-1.69E-46
MS.gene050128	MS.gene070950	0.821868	3.21E-53	-1.69E-46
MS.gene050301	MS.gene070950	0.817048	4.04E-52	-1.69E-46
MS.gene050432	MS.gene070950	0.816077	6.66E-52	-1.69E-46
MS.gene050670	MS.gene070950	0.843994	9.95E-59	-1.69E-46
MS.gene050971	MS.gene070950	0.813676	2.27E-51	-1.69E-46
MS.gene05105	MS.gene070950	0.83593	1.26E-56	-1.69E-46
MS.gene051360	MS.gene070950	0.827275	1.71E-54	-1.69E-46
MS.gene051692	MS.gene070950	0.813262	2.80E-51	-1.69E-46
MS.gene051860	MS.gene070950	0.805918	1.06E-49	-1.69E-46
MS.gene052135	MS.gene070950	0.806462	8.13E-50	-1.69E-46
MS.gene052136	MS.gene070950	0.806924	6.49E-50	-1.69E-46
MS.gene052188	MS.gene070950	0.847864	8.83E-60	-1.69E-46
MS.gene052253	MS.gene070950	0.851978	6.24E-61	-1.69E-46
MS.gene052310	MS.gene070950	0.812163	4.87E-51	-1.69E-46
MS.gene052340	MS.gene070950	0.838956	2.11E-57	-1.69E-46
MS.gene052414	MS.gene070950	0.819118	1.37E-52	-1.69E-46
MS.gene052421	MS.gene070950	0.817967	2.50E-52	-1.69E-46
MS.gene052481	MS.gene070950	0.805833	1.10E-49	-1.69E-46
MS.gene052485	MS.gene070950	0.845914	3.02E-59	-1.69E-46
MS.gene053073	MS.gene070950	0.806987	6.30E-50	-1.69E-46
MS.gene053103	MS.gene070950	0.805883	1.08E-49	-1.69E-46
MS.gene05316	MS.gene070950	0.841075	5.92E-58	-1.69E-46
MS.gene053209	MS.gene070950	0.827115	1.87E-54	-1.69E-46
MS.gene053449	MS.gene070950	0.864496	1.17E-64	-1.69E-46
MS.gene053557	MS.gene070950	0.814756	1.31E-51	-1.69E-46
MS.gene053758	MS.gene070950	0.815305	9.90E-52	-1.69E-46
MS.gene053813	MS.gene070950	0.833854	4.20E-56	-1.69E-46
MS.gene053912	MS.gene070950	0.856354	3.41E-62	-1.69E-46
MS.gene054183	MS.gene070950	0.81914	1.36E-52	-1.69E-46
MS.gene054918	MS.gene070950	0.804444	2.15E-49	-1.69E-46
MS.gene05505	MS.gene070950	0.813564	2.40E-51	-1.69E-46
MS.gene055116	MS.gene070950	0.865924	4.15E-65	-1.69E-46
MS.gene055666	MS.gene070950	0.817534	3.14E-52	-1.69E-46
MS.gene055909	MS.gene070950	0.819236	1.29E-52	-1.69E-46
MS.gene055911	MS.gene070950	0.818007	2.45E-52	-1.69E-46
MS.gene05592	MS.gene070950	0.802381	5.76E-49	-1.69E-46
MS.gene056191	MS.gene070950	0.815226	1.03E-51	-1.69E-46
MS.gene056407	MS.gene070950	0.816383	5.69E-52	-1.69E-46
MS.gene056422	MS.gene070950	0.860319	2.24E-63	-1.69E-46
MS.gene056511	MS.gene070950	0.879731	9.80E-70	-1.69E-46
MS.gene056659	MS.gene070950	0.816427	5.56E-52	-1.69E-46
MS.gene05702	MS.gene070950	0.827218	1.77E-54	-1.69E-46
MS.gene057124	MS.gene070950	0.817354	3.44E-52	-1.69E-46
MS.gene057146	MS.gene070950	0.800824	1.20E-48	-1.69E-46
MS.gene057310	MS.gene070950	0.81085	9.39E-51	-1.69E-46
MS.gene057396	MS.gene070950	0.825578	4.34E-54	-1.69E-46
MS.gene057448	MS.gene070950	0.853767	1.92E-61	-1.69E-46
MS.gene057449	MS.gene070950	0.861233	1.18E-63	-1.69E-46
MS.gene057450	MS.gene070950	0.852438	4.62E-61	-1.69E-46
MS.gene057537	MS.gene070950	0.823459	1.37E-53	-1.69E-46
MS.gene057734	MS.gene070950	0.826604	2.48E-54	-1.69E-46
MS.gene057749	MS.gene070950	0.814659	1.38E-51	-1.69E-46
MS.gene057823	MS.gene070950	0.814337	1.62E-51	-1.69E-46
MS.gene058007	MS.gene070950	0.821813	3.31E-53	-1.69E-46
MS.gene058075	MS.gene070950	0.821485	3.94E-53	-1.69E-46
MS.gene05842	MS.gene070950	0.808547	2.94E-50	-1.69E-46
MS.gene058459	MS.gene070950	0.833688	4.62E-56	-1.69E-46
MS.gene058928	MS.gene070950	0.863751	1.99E-64	-1.69E-46
MS.gene058995	MS.gene070950	0.809212	2.12E-50	-1.69E-46
MS.gene05907	MS.gene070950	0.815682	8.16E-52	-1.69E-46
MS.gene059082	MS.gene070950	0.803221	3.87E-49	-1.69E-46
MS.gene059285	MS.gene070950	0.813543	2.43E-51	-1.69E-46
MS.gene059294	MS.gene070950	0.803427	3.50E-49	-1.69E-46
MS.gene059366	MS.gene070950	0.868016	8.93E-66	-1.69E-46
MS.gene059368	MS.gene070950	0.811122	8.20E-51	-1.69E-46
MS.gene059420	MS.gene070950	0.855369	6.61E-62	-1.69E-46
MS.gene059597	MS.gene070950	0.807639	4.58E-50	-1.69E-46
MS.gene059674	MS.gene070950	0.820223	7.68E-53	-1.69E-46
MS.gene059676	MS.gene070950	0.80167	8.07E-49	-1.69E-46
MS.gene059688	MS.gene070950	0.862832	3.83E-64	-1.69E-46
MS.gene059692	MS.gene070950	0.845264	4.53E-59	-1.69E-46
MS.gene059695	MS.gene070950	0.842488	2.51E-58	-1.69E-46
MS.gene059711	MS.gene070950	0.855481	6.13E-62	-1.69E-46
MS.gene059807	MS.gene070950	0.906663	1.17E-80	-1.69E-46
MS.gene05984	MS.gene070950	0.804234	2.38E-49	-1.69E-46
MS.gene059876	MS.gene070950	0.832029	1.19E-55	-1.69E-46
MS.gene059933	MS.gene070950	0.803067	4.16E-49	-1.69E-46
MS.gene060247	MS.gene070950	0.80785	4.13E-50	-1.69E-46
MS.gene06037	MS.gene070950	0.803602	3.22E-49	-1.69E-46
MS.gene060403	MS.gene070950	0.829475	5.04E-55	-1.69E-46
MS.gene060495	MS.gene070950	0.84378	1.14E-58	-1.69E-46
MS.gene060818	MS.gene070950	0.813641	2.31E-51	-1.69E-46
MS.gene060837	MS.gene070950	0.864329	1.32E-64	-1.69E-46
MS.gene060927	MS.gene070950	0.800553	1.37E-48	-1.69E-46
MS.gene060986	MS.gene070950	0.810205	1.29E-50	-1.69E-46
MS.gene061041	MS.gene070950	0.805493	1.30E-49	-1.69E-46
MS.gene061049	MS.gene070950	0.841488	4.61E-58	-1.69E-46
MS.gene061051	MS.gene070950	-0.833755	4.45E-56	-1.69E-46
MS.gene061154	MS.gene070950	0.823622	1.25E-53	-1.69E-46
MS.gene061159	MS.gene070950	0.844348	7.99E-59	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070950.t1	MTR_1g103050	98.611	72	1	0	1	72	1	72	3.90e-48	149

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 16 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTGCATCCAATGATTCCTTT+AGG	0.352281	1.4:+79715791	None:intergenic
CTCTTACCTAAAGGAATCAT+TGG	0.362848	1.4:-79715797	MS.gene070950:CDS
TTTGAAAGCAGTTTCATCTA+AGG	0.366099	1.4:+79715747	None:intergenic
TTTCTTCTGTAGGCGTTGTT+TGG	0.381475	1.4:+79715723	None:intergenic
TGAATGGCTGGTAGGCTACC+TGG	0.496595	1.4:-79715892	MS.gene070950:CDS
CAACGCCTACAGAAGAAACT+TGG	0.500663	1.4:-79715718	MS.gene070950:CDS
GCTGGTAGGCTACCTGGCGT+TGG	0.518244	1.4:-79715886	MS.gene070950:CDS
GGATTTGATGTACCTGGAAG+AGG	0.518796	1.4:+79715684	None:intergenic
GGTAAAGAAGAGTGAATGGC+TGG	0.520572	1.4:-79715904	None:intergenic
CTACAGAGACTCTTACCTAA+AGG	0.529350	1.4:-79715806	MS.gene070950:CDS
AGTGACCAAGTTTCTTCTGT+AGG	0.561380	1.4:+79715713	None:intergenic
CTTGGTGACAATCCAACGCC+AGG	0.563291	1.4:+79715874	None:intergenic
GTAGTAGTCATTGAAGTCCA+TGG	0.592320	1.4:+79715769	None:intergenic
AAGAAGAGTGAATGGCTGGT+AGG	0.606425	1.4:-79715900	None:intergenic
CTTTAGGTAAGAGTCTCTGT+AGG	0.619274	1.4:+79715807	None:intergenic
AGGAATCATTGGATGCACCA+TGG	0.643184	1.4:-79715786	MS.gene070950:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAACTGTATTATTATGT+AGG	+	chr1.4:79715663-79715682	None:intergenic	15.0%
!!!	ATTTATTTTTTTTCTCTCTA+AGG	-	chr1.4:79715886-79715905	MS.gene070950:CDS	15.0%
!!!	TTTTTTGTTACAAAAAGTTT+AGG	-	chr1.4:79715752-79715771	MS.gene070950:CDS	15.0%
!!	AGAATTTATACAAATTGTCT+AGG	-	chr1.4:79715804-79715823	MS.gene070950:CDS	20.0%
!!!	TAATTTTCTGATCTTTTTCT+TGG	+	chr1.4:79715470-79715489	None:intergenic	20.0%
!!!	TTTTTGAAGAAACAAGAAAT+TGG	-	chr1.4:79715686-79715705	MS.gene070950:intron	20.0%
!	TTGTTACAAAAAGTTTAGGT+GGG	-	chr1.4:79715756-79715775	MS.gene070950:CDS	25.0%
!	TTTGTTACAAAAAGTTTAGG+TGG	-	chr1.4:79715755-79715774	MS.gene070950:CDS	25.0%
!!	AGTATAAATTCTAGCTAGTT+AGG	+	chr1.4:79715729-79715748	None:intergenic	25.0%
!!	ATTCTAGCTAGTTAACAAAA+AGG	+	chr1.4:79715789-79715808	None:intergenic	25.0%
	TTTGAAAGCAGTTTCATCTA+AGG	+	chr1.4:79715579-79715598	None:intergenic	30.0%
	CTCTTACCTAAAGGAATCAT+TGG	-	chr1.4:79715526-79715545	MS.gene070950:intron	35.0%
	GAGTGTGCATAAGAAATATG+TGG	+	chr1.4:79715845-79715864	None:intergenic	35.0%
	TATGTAGGATTTGATGTACC+TGG	+	chr1.4:79715648-79715667	None:intergenic	35.0%
!	ACAAAAAGTTTAGGTGGGTT+CGG	-	chr1.4:79715761-79715780	MS.gene070950:CDS	35.0%
	AGTGACCAAGTTTCTTCTGT+AGG	+	chr1.4:79715613-79715632	None:intergenic	40.0%
	CTACAGAGACTCTTACCTAA+AGG	-	chr1.4:79715517-79715536	MS.gene070950:intron	40.0%
	CTTTAGGTAAGAGTCTCTGT+AGG	+	chr1.4:79715519-79715538	None:intergenic	40.0%
	GTAGTAGTCATTGAAGTCCA+TGG	+	chr1.4:79715557-79715576	None:intergenic	40.0%
	GTGCATCCAATGATTCCTTT+AGG	+	chr1.4:79715535-79715554	None:intergenic	40.0%
!	TTTCTTCTGTAGGCGTTGTT+TGG	+	chr1.4:79715603-79715622	None:intergenic	40.0%
	AGGAATCATTGGATGCACCA+TGG	-	chr1.4:79715537-79715556	MS.gene070950:intron	45.0%
	CAACGCCTACAGAAGAAACT+TGG	-	chr1.4:79715605-79715624	MS.gene070950:intron	45.0%
	GCACACTCACATGCATAGTA+AGG	-	chr1.4:79715857-79715876	MS.gene070950:CDS	45.0%
	GGATTTGATGTACCTGGAAG+AGG	+	chr1.4:79715642-79715661	None:intergenic	45.0%
!	GTCACTTCTTTTCCTCTTCC+AGG	-	chr1.4:79715627-79715646	MS.gene070950:intron	45.0%
!	CTTGGTGACAATCCAACGCC+AGG	+	chr1.4:79715452-79715471	None:intergenic	55.0%
	GCTGGTAGGCTACCTGGCGT+TGG	-	chr1.4:79715437-79715456	MS.gene070950:intron	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	79715434	79715911	79715434	ID=MS.gene070950
chr1.4	mRNA	79715434	79715911	79715434	ID=MS.gene070950.t1;Parent=MS.gene070950
chr1.4	exon	79715697	79715911	79715697	ID=MS.gene070950.t1.exon1;Parent=MS.gene070950.t1
chr1.4	CDS	79715697	79715911	79715697	ID=cds.MS.gene070950.t1;Parent=MS.gene070950.t1
chr1.4	exon	79715434	79715437	79715434	ID=MS.gene070950.t1.exon2;Parent=MS.gene070950.t1
chr1.4	CDS	79715434	79715437	79715434	ID=cds.MS.gene070950.t1;Parent=MS.gene070950.t1

Gene Sequence

>MS.gene070950

ATGGCTGGTAGGCTACCTGGCGTTGGATTGTCACCAAGAAAAAGATCAGAAAATTATCACAACAGACATGAACATTATGCTACCTACAGAGACTCTTACCTAAAGGAATCATTGGATGCACCATGGACTTCAATGACTACTACCTTAGATGAAACTGCTTTCAAAGCCAAACAACGCCTACAGAAGAAACTTGGTCACTTCTTTTCCTCTTCCAGGTACATCAAATCCTACATAATAATACAGTTTTTTTATTTTTTGAAGAAACAAGAAATTGGTTACTATTAATTTTGAGCCTAACTAGCTAGAATTTATACTTTTTTTTTTGTTACAAAAAGTTTAGGTGGGTTCGGCTCCTTTTTGTTAACTAGCTAGAATTTATACAAATTGTCTAGGCTTTTATTTAATTTTCCACATATTTCTTATGCACACTCACATGCATAGTAAGGAATTTAATTTATTTTTTTTCTCTCTAAGGTGA

Protein sequence

>MS.gene070950.t1

MAGRLPGVGLSPRKRSENYHNRHEHYATYRDSYLKESLDAPWTSMTTTLDETAFKAKQRLQKKLGHFFSSSR