AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072424


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072424.t1 MTR_7g114210 97.143 175 5 0 1 175 1 175 5.13e-128 357
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072424.t1 AT3G05000 79.429 175 33 2 1 175 1 172 2.63e-101 289

Find 46 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCTCGGTTTGGTGATCATT+TGG 0.323253 7.3:+2824171 MS.gene072424:CDS
GATCGAAGCCATTGGTTATC+AGG 0.347972 7.3:+2823781 MS.gene072424:CDS
ATCCTGCGAGCAGCGAGTTC+AGG 0.362814 7.3:-2823761 None:intergenic
TTCCCATGCGGGATCATTAG+AGG 0.368149 7.3:+2824582 MS.gene072424:CDS
GATATACGATGGAGCGGCCT+CGG 0.415640 7.3:+2824155 MS.gene072424:intron
TAGAGGAGCTCTTTCCAACT+TGG 0.425869 7.3:+2824599 MS.gene072424:CDS
TGCACCTACGGCTGAAAGTA+AGG 0.438829 7.3:+2824530 MS.gene072424:CDS
CTAGAGTAGTTCATACTCAA+TGG 0.454113 7.3:+2824874 MS.gene072424:CDS
GGCAATTAAGTTCATTTGCA+AGG 0.458740 7.3:+2824195 MS.gene072424:CDS
AGCAGCGAGTTCAGGTTTGT+TGG 0.468848 7.3:-2823753 None:intergenic
GGCTGAAAGTAAGGCAGCAC+AGG 0.488115 7.3:+2824539 MS.gene072424:CDS
ACGATGGAGCGGCCTCGGTT+TGG 0.496970 7.3:+2824160 MS.gene072424:CDS
ATAAATTTCCGTGGCTTGCA+CGG 0.497008 7.3:+2824457 MS.gene072424:CDS
AGAGGAGCTCTTTCCAACTT+GGG 0.498177 7.3:+2824600 MS.gene072424:CDS
TGCATCTTTATTTCCCATGC+GGG 0.509752 7.3:+2824571 MS.gene072424:CDS
TAGAGTAGTTCATACTCAAT+GGG 0.517531 7.3:+2824875 MS.gene072424:CDS
TCCGTGGCTTGCACGGATGT+CGG 0.520572 7.3:+2824464 MS.gene072424:CDS
TCGGTTTGGTGATCATTTGG+AGG 0.520959 7.3:+2824174 MS.gene072424:CDS
ACAACTTGAAAACTAACCAT+AGG 0.528003 7.3:+2824251 MS.gene072424:CDS
AACCTGAACTCGCTGCTCGC+AGG 0.543806 7.3:+2823759 MS.gene072424:CDS
CAACTTGAAAACTAACCATA+GGG 0.547452 7.3:+2824252 MS.gene072424:CDS
TGATAATGTTAGTTCAGTTG+AGG 0.553497 7.3:+2824500 MS.gene072424:CDS
TGCACAGGGAATTCCCAAGT+TGG 0.556321 7.3:-2824613 None:intergenic
TGAGGATTATTCTGCACCTA+CGG 0.558019 7.3:+2824518 MS.gene072424:CDS
GCTGCCTTACTTTCAGCCGT+AGG 0.558260 7.3:-2824534 None:intergenic
ACCGACATCCGTGCAAGCCA+CGG 0.566327 7.3:-2824465 None:intergenic
TATGTCTGCAGAAACTGCAC+AGG 0.568585 7.3:-2824628 None:intergenic
TTCAGAGAGTGAAAATGGTG+AGG 0.569906 7.3:+2823660 None:intergenic
GAACTAACATTATCAGTGGA+TGG 0.571137 7.3:-2824492 None:intergenic
TTGCGTTGATAGCTTGATGA+CGG 0.587460 7.3:+2823697 MS.gene072424:CDS
GCTCCTCTAATGATCCCGCA+TGG 0.591236 7.3:-2824585 None:intergenic
CTCCTCTAATGATCCCGCAT+GGG 0.596153 7.3:-2824584 None:intergenic
TGCAAGATAATAAATTTCCG+TGG 0.603647 7.3:+2824448 MS.gene072424:CDS
TCAGAGAGTGAAAATGGTGA+GGG 0.605160 7.3:+2823661 None:intergenic
TCGGTCAACAGCTCTCTGAA+AGG 0.608978 7.3:+2823804 MS.gene072424:CDS
GCTCGCAGGATCGAAGCCAT+TGG 0.613190 7.3:+2823773 MS.gene072424:CDS
GGGGATACACACATGCTGGA+AGG 0.619761 7.3:-2824656 None:intergenic
GAAGCCATTGGTTATCAGGT+CGG 0.622295 7.3:+2823785 MS.gene072424:CDS
AACTGAACTAACATTATCAG+TGG 0.624516 7.3:-2824496 None:intergenic
TTGACCGACCTGATAACCAA+TGG 0.626958 7.3:-2823789 None:intergenic
TGTGTAATTCAGATATACGA+TGG 0.627817 7.3:+2824144 MS.gene072424:intron
TGATAGCTTGATGACGGAGA+TGG 0.641800 7.3:+2823703 MS.gene072424:CDS
ATGCATCTTTATTTCCCATG+CGG 0.650443 7.3:+2824570 MS.gene072424:CDS
AATTCAGATATACGATGGAG+CGG 0.678919 7.3:+2824149 MS.gene072424:intron
TCCAAATGATCACCAAACCG+AGG 0.695841 7.3:-2824172 None:intergenic
ATGTCTGCAGAAACTGCACA+GGG 0.724210 7.3:-2824627 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATATATATATATATA+TGG + chr7.3:2824295-2824314 MS.gene072424:intron 0.0%
!!! TTCTTGATATTAATTATTTT+TGG + chr7.3:2823902-2823921 MS.gene072424:intron 10.0%
!!! TGATATTAATTATTTTTGGT+AGG + chr7.3:2823906-2823925 MS.gene072424:intron 15.0%
!! TCATGAAATTGTAATCAAAT+TGG + chr7.3:2823951-2823970 MS.gene072424:intron 20.0%
!!! TTTTCATTTTTAGTGTTTTC+TGG + chr7.3:2824783-2824802 MS.gene072424:intron 20.0%
! TGTGAATAATTTGATCTGTT+TGG + chr7.3:2824834-2824853 MS.gene072424:intron 25.0%
! TGTTTGGAAATGTTTAAACA+GGG + chr7.3:2824850-2824869 MS.gene072424:intron 25.0%
!! ATTTTCTCGAATTACTACAT+AGG - chr7.3:2824019-2824038 None:intergenic 25.0%
!! CATAGAAGAGAAGATTTTTA+AGG + chr7.3:2824745-2824764 MS.gene072424:intron 25.0%
!!! TTTTTGTGTTTTGATTTCCA+TGG + chr7.3:2824388-2824407 MS.gene072424:intron 25.0%
AACTGAACTAACATTATCAG+TGG - chr7.3:2824499-2824518 None:intergenic 30.0%
ACAACTTGAAAACTAACCAT+AGG + chr7.3:2824251-2824270 MS.gene072424:CDS 30.0%
AGATGAAGAAAGAGAGAATT+AGG - chr7.3:2823845-2823864 None:intergenic 30.0%
CAACTTGAAAACTAACCATA+GGG + chr7.3:2824252-2824271 MS.gene072424:CDS 30.0%
CTGTTTGGAAATGTTTAAAC+AGG + chr7.3:2824849-2824868 MS.gene072424:intron 30.0%
GATGAAGAAAGAGAGAATTA+GGG - chr7.3:2823844-2823863 None:intergenic 30.0%
TAGAGTAGTTCATACTCAAT+GGG + chr7.3:2824875-2824894 MS.gene072424:CDS 30.0%
TGCAAGATAATAAATTTCCG+TGG + chr7.3:2824448-2824467 MS.gene072424:CDS 30.0%
TGTGTAATTCAGATATACGA+TGG + chr7.3:2824144-2824163 MS.gene072424:intron 30.0%
! AGTTCATTTGCAAGGATTTT+TGG + chr7.3:2824203-2824222 MS.gene072424:CDS 30.0%
! ATTTCCATGGTTGTGTTATT+TGG + chr7.3:2824401-2824420 MS.gene072424:intron 30.0%
! TGATAATGTTAGTTCAGTTG+AGG + chr7.3:2824500-2824519 MS.gene072424:CDS 30.0%
AATTCAGATATACGATGGAG+CGG + chr7.3:2824149-2824168 MS.gene072424:intron 35.0%
AGAACCAAATAACACAACCA+TGG - chr7.3:2824408-2824427 None:intergenic 35.0%
CTAGAGTAGTTCATACTCAA+TGG + chr7.3:2824874-2824893 MS.gene072424:CDS 35.0%
CTGTTATCACACAACATAGT+AGG + chr7.3:2823869-2823888 MS.gene072424:intron 35.0%
GAACTAACATTATCAGTGGA+TGG - chr7.3:2824495-2824514 None:intergenic 35.0%
GGCAATTAAGTTCATTTGCA+AGG + chr7.3:2824195-2824214 MS.gene072424:CDS 35.0%
! ATGCATCTTTATTTCCCATG+CGG + chr7.3:2824570-2824589 MS.gene072424:CDS 35.0%
AACCAATTCAACTCACCCTA+TGG - chr7.3:2824270-2824289 None:intergenic 40.0%
GAATGTAGTCACATGTGTCA+GGG + chr7.3:2824048-2824067 MS.gene072424:intron 40.0%
TGAATGTAGTCACATGTGTC+AGG + chr7.3:2824047-2824066 MS.gene072424:intron 40.0%
TGAGGATTATTCTGCACCTA+CGG + chr7.3:2824518-2824537 MS.gene072424:CDS 40.0%
! AACCATAGGGTGAGTTGAAT+TGG + chr7.3:2824265-2824284 MS.gene072424:intron 40.0%
! ATAAATTTCCGTGGCTTGCA+CGG + chr7.3:2824457-2824476 MS.gene072424:CDS 40.0%
! GTCAGATAGGTTATGATGGA+TGG + chr7.3:2824070-2824089 MS.gene072424:intron 40.0%
! TGCATCTTTATTTCCCATGC+GGG + chr7.3:2824571-2824590 MS.gene072424:CDS 40.0%
!! TGTTATTTGGTTCTGCTGCA+GGG + chr7.3:2824414-2824433 MS.gene072424:intron 40.0%
!! TTGCGTTGATAGCTTGATGA+CGG + chr7.3:2823697-2823716 MS.gene072424:CDS 40.0%
!!! TTGCAAGGATTTTTGGTCTG+AGG + chr7.3:2824210-2824229 MS.gene072424:CDS 40.0%
ACTACATAGGTCGTGCTTCA+TGG - chr7.3:2824006-2824025 None:intergenic 45.0%
AGAGGAGCTCTTTCCAACTT+GGG + chr7.3:2824600-2824619 MS.gene072424:CDS 45.0%
ATGTCTGCAGAAACTGCACA+GGG - chr7.3:2824630-2824649 None:intergenic 45.0%
CAGGGTCAGATAGGTTATGA+TGG + chr7.3:2824066-2824085 MS.gene072424:intron 45.0%
TAGAGGAGCTCTTTCCAACT+TGG + chr7.3:2824599-2824618 MS.gene072424:CDS 45.0%
TATGTCTGCAGAAACTGCAC+AGG - chr7.3:2824631-2824650 None:intergenic 45.0%
TCCAAATGATCACCAAACCG+AGG - chr7.3:2824175-2824194 None:intergenic 45.0%
TTGACCGACCTGATAACCAA+TGG - chr7.3:2823792-2823811 None:intergenic 45.0%
! GAAGCCATTGGTTATCAGGT+CGG + chr7.3:2823785-2823804 MS.gene072424:CDS 45.0%
! GATCGAAGCCATTGGTTATC+AGG + chr7.3:2823781-2823800 MS.gene072424:CDS 45.0%
! TCAACAGCTCTCTGAAAGGT+TGG + chr7.3:2823808-2823827 MS.gene072424:intron 45.0%
!! GTGTTATTTGGTTCTGCTGC+AGG + chr7.3:2824413-2824432 MS.gene072424:intron 45.0%
!! TCGGTTTGGTGATCATTTGG+AGG + chr7.3:2824174-2824193 MS.gene072424:CDS 45.0%
!! TGATAGCTTGATGACGGAGA+TGG + chr7.3:2823703-2823722 MS.gene072424:CDS 45.0%
AGAGAGAGAGAGAGAGAGAG+AGG - chr7.3:2824687-2824706 None:intergenic 50.0%
AGAGAGAGAGAGAGGGAGAT+GGG - chr7.3:2824681-2824700 None:intergenic 50.0%
CACATGTGTCAGGGTCAGAT+AGG + chr7.3:2824057-2824076 MS.gene072424:intron 50.0%
CTCCTCTAATGATCCCGCAT+GGG - chr7.3:2824587-2824606 None:intergenic 50.0%
GAGAGAGAGAGAGAGAGAGA+GGG - chr7.3:2824688-2824707 None:intergenic 50.0%
TCGGTCAACAGCTCTCTGAA+AGG + chr7.3:2823804-2823823 MS.gene072424:CDS 50.0%
TGCACAGGGAATTCCCAAGT+TGG - chr7.3:2824616-2824635 None:intergenic 50.0%
TGCACCTACGGCTGAAAGTA+AGG + chr7.3:2824530-2824549 MS.gene072424:CDS 50.0%
TTCCCATGCGGGATCATTAG+AGG + chr7.3:2824582-2824601 MS.gene072424:CDS 50.0%
! AGCAGCGAGTTCAGGTTTGT+TGG - chr7.3:2823756-2823775 None:intergenic 50.0%
!! GCCTCGGTTTGGTGATCATT+TGG + chr7.3:2824171-2824190 MS.gene072424:CDS 50.0%
AGAGAGAGAGAGGGAGATGG+GGG - chr7.3:2824679-2824698 None:intergenic 55.0%
ATGGGGGGATACACACATGC+TGG - chr7.3:2824663-2824682 None:intergenic 55.0%
GAGAGAGAGAGAGAGGGAGA+TGG - chr7.3:2824682-2824701 None:intergenic 55.0%
GAGAGAGAGAGAGGGAGATG+GGG - chr7.3:2824680-2824699 None:intergenic 55.0%
GATATACGATGGAGCGGCCT+CGG + chr7.3:2824155-2824174 MS.gene072424:intron 55.0%
GCTCCTCTAATGATCCCGCA+TGG - chr7.3:2824588-2824607 None:intergenic 55.0%
GCTGCCTTACTTTCAGCCGT+AGG - chr7.3:2824537-2824556 None:intergenic 55.0%
GGCTGAAAGTAAGGCAGCAC+AGG + chr7.3:2824539-2824558 MS.gene072424:CDS 55.0%
GGGGATACACACATGCTGGA+AGG - chr7.3:2824659-2824678 None:intergenic 55.0%
AACCTGAACTCGCTGCTCGC+AGG + chr7.3:2823759-2823778 MS.gene072424:CDS 60.0%
ACCGACATCCGTGCAAGCCA+CGG - chr7.3:2824468-2824487 None:intergenic 60.0%
ATCCTGCGAGCAGCGAGTTC+AGG - chr7.3:2823764-2823783 None:intergenic 60.0%
GAGAGAGAGAGGGAGATGGG+GGG - chr7.3:2824678-2824697 None:intergenic 60.0%
GCTCGCAGGATCGAAGCCAT+TGG + chr7.3:2823773-2823792 MS.gene072424:CDS 60.0%
! TCCGTGGCTTGCACGGATGT+CGG + chr7.3:2824464-2824483 MS.gene072424:CDS 60.0%
ACGATGGAGCGGCCTCGGTT+TGG + chr7.3:2824160-2824179 MS.gene072424:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 2823674 2824923 2823674 ID=MS.gene072424
chr7.3 mRNA 2823674 2824923 2823674 ID=MS.gene072424.t1;Parent=MS.gene072424
chr7.3 exon 2823674 2823825 2823674 ID=MS.gene072424.t1.exon1;Parent=MS.gene072424.t1
chr7.3 CDS 2823674 2823825 2823674 ID=cds.MS.gene072424.t1;Parent=MS.gene072424.t1
chr7.3 exon 2824156 2824273 2824156 ID=MS.gene072424.t1.exon2;Parent=MS.gene072424.t1
chr7.3 CDS 2824156 2824273 2824156 ID=cds.MS.gene072424.t1;Parent=MS.gene072424.t1
chr7.3 exon 2824435 2824666 2824435 ID=MS.gene072424.t1.exon3;Parent=MS.gene072424.t1
chr7.3 CDS 2824435 2824666 2824435 ID=cds.MS.gene072424.t1;Parent=MS.gene072424.t1
chr7.3 exon 2824871 2824923 2824871 ID=MS.gene072424.t1.exon4;Parent=MS.gene072424.t1
chr7.3 CDS 2824871 2824923 2824871 ID=cds.MS.gene072424.t1;Parent=MS.gene072424.t1
Gene Sequence

>MS.gene072424

ATGGTGAGGGAAGTTTCAGAGAGTTGCGTTGATAGCTTGATGACGGAGATGGTTGCTTGTTATTGCAATCGATTCTATGCCAACAAACCTGAACTCGCTGCTCGCAGGATCGAAGCCATTGGTTATCAGGTCGGTCAACAGCTCTCTGAAAGGTTGGTTGTAACAAACCCTAATTCTCTCTTTCTTCATCTAGATCTGTTATCACACAACATAGTAGGATTATTCTATTTCTTGATATTAATTATTTTTGGTAGGTTGATTCTGTATTGCTTTTGTATCATGAAATTGTAATCAAATTGGTTAGACTATGATTGATTAGTTGTTATTATCCATGAAGCACGACCTATGTAGTAATTCGAGAAAATGAAATAATTGAATGTAGTCACATGTGTCAGGGTCAGATAGGTTATGATGGATGGTTCACTGAAGTAAGTTATGATTAAATTGCAATGAATTGAATAAATGTGATGTGTGTAATTCAGATATACGATGGAGCGGCCTCGGTTTGGTGATCATTTGGAGGCAATTAAGTTCATTTGCAAGGATTTTTGGTCTGAGGTGTTTAAGAAGCAGATAGACAACTTGAAAACTAACCATAGGGTGAGTTGAATTGGTTGAATGTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATGGTATTTTTGTGTTTTGATTTCCATGGTTGTGTTATTTGGTTCTGCTGCAGGGTACATTTGTGTTGCAAGATAATAAATTTCCGTGGCTTGCACGGATGTCGGTTGATCCATCCACTGATAATGTTAGTTCAGTTGAGGATTATTCTGCACCTACGGCTGAAAGTAAGGCAGCACAGGCAATGAGCATGCATCTTTATTTCCCATGCGGGATCATTAGAGGAGCTCTTTCCAACTTGGGAATTCCCTGTGCAGTTTCTGCAGACATATCAAGCCTTCCAGCATGTGTGTATCCCCCCATCTCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCACACACACACACACACACACACATAGAAGAGAAGATTTTTAAGGATAGCAAAGAGTTTCTTTTCATTTTTAGTGTTTTCTGGATGCACAATTTGCATGTGACAAGTCTGATGTGAATAATTTGATCTGTTTGGAAATGTTTAAACAGGGTCTAGAGTAGTTCATACTCAATGGGTTACAGTGAGAAGTTCAAGAGTGTGA

Protein sequence

>MS.gene072424.t1

MVREVSESCVDSLMTEMVACYCNRFYANKPELAARRIEAIGYQVGQQLSERYTMERPRFGDHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKFPWLARMSVDPSTDNVSSVEDYSAPTAESKAAQAMSMHLYFPCGIIRGALSNLGIPCAVSADISSLPAWSRVVHTQWVTVRSSRV