AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene09022


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09022.t1 MTR_8g080750 90.698 43 4 0 1 43 181 223 5.76e-20 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09022.t1 AT1G27330 84.906 53 8 0 1 53 1 53 3.23e-27 93.6
MS.gene09022.t1 AT1G27350 84.906 53 8 0 1 53 1 53 3.23e-27 93.6

Find 16 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTTCTTAACAGTTGTTTC+TGG 0.223249 4.4:-15330259 None:intergenic
TTCTTCGTCTTTGTTGTCAT+TGG 0.272950 4.4:+15330316 MS.gene09022:CDS
ACGAAGAAACCAAGCAAAAC+GGG 0.342450 4.4:-15330301 None:intergenic
CAGCTCTCTTTCAGATAATC+AGG 0.365308 4.4:+15331264 MS.gene09022:intron
CCAAGCAAAACGGGGCCAAC+AGG 0.391773 4.4:-15330292 None:intergenic
GACGAAGAAACCAAGCAAAA+CGG 0.413008 4.4:-15330302 None:intergenic
CATCAAAGCGCCTTGCAGTT+AGG 0.424154 4.4:+15330191 MS.gene09022:CDS
CGAGTGGAGGCATGGCATAA+TGG 0.448620 4.4:+15331294 MS.gene09022:CDS
CCAGAAACAACTGTTAAGAA+AGG 0.524644 4.4:+15330259 MS.gene09022:CDS
AAAGGAAATGACTATCCTGT+TGG 0.571369 4.4:+15330277 MS.gene09022:CDS
GACAGCAACGAGTGGAGGCA+TGG 0.571659 4.4:+15331286 MS.gene09022:CDS
AAAGCGCCTTGCAGTTAGGA+AGG 0.575086 4.4:+15330195 MS.gene09022:CDS
ATCAGGACAGCAACGAGTGG+AGG 0.608196 4.4:+15331281 MS.gene09022:CDS
CGAAGAAACCAAGCAAAACG+GGG 0.617394 4.4:-15330300 None:intergenic
TCTCAACCTTCCTAACTGCA+AGG 0.647474 4.4:-15330201 None:intergenic
ATAATCAGGACAGCAACGAG+TGG 0.658965 4.4:+15331278 MS.gene09022:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTAAATAAAAAAAAATTACA+GGG - chr4.4:15330713-15330732 None:intergenic 10.0%
!! TAAATAAAAAAAAATTACAG+GGG - chr4.4:15330712-15330731 None:intergenic 10.0%
!! AAAAATTAGTAAAACACATA+AGG - chr4.4:15330374-15330393 None:intergenic 15.0%
!! AAAAATTTACTATATTACAG+AGG - chr4.4:15330670-15330689 None:intergenic 15.0%
!! AAAATTTACTATATTACAGA+GGG - chr4.4:15330669-15330688 None:intergenic 15.0%
!! AAATAAAAAAAAATTACAGG+GGG - chr4.4:15330711-15330730 None:intergenic 15.0%
!! ATCAGAAATTTATCAAAATT+AGG + chr4.4:15329980-15329999 MS.gene09022:intron 15.0%
!! CAATATTTCAAAAATTGAAT+CGG - chr4.4:15330624-15330643 None:intergenic 15.0%
!! GGTAAATAAAAAAAAATTAC+AGG - chr4.4:15330714-15330733 None:intergenic 15.0%
!! TCAATTTCAAAACAAAAATT+CGG - chr4.4:15329917-15329936 None:intergenic 15.0%
!!! AAGAAATTTTTGTTTTTGTT+TGG - chr4.4:15330776-15330795 None:intergenic 15.0%
!!! AATTTTTGAAATATTGTTGT+GGG + chr4.4:15330628-15330647 MS.gene09022:intron 15.0%
!!! ATAATTTTCAATTCAATTCA+AGG - chr4.4:15330927-15330946 None:intergenic 15.0%
!!! TATATTAATGTGTTTGAAAT+TGG - chr4.4:15330844-15330863 None:intergenic 15.0%
!!! TGTTAAATTTTGATTGAATT+TGG + chr4.4:15330102-15330121 MS.gene09022:intron 15.0%
!! AAAACAAAAAATTTGTACAG+GGG - chr4.4:15330735-15330754 None:intergenic 20.0%
!! CAAAACAAAAAATTTGTACA+GGG - chr4.4:15330736-15330755 None:intergenic 20.0%
!! TATTGTTTGTGTGATTTATT+TGG + chr4.4:15330443-15330462 MS.gene09022:intron 20.0%
!! TCAAAACAAAAAATTTGTAC+AGG - chr4.4:15330737-15330756 None:intergenic 20.0%
!!! ATTTTTGAAATATTGTTGTG+GGG + chr4.4:15330629-15330648 MS.gene09022:intron 20.0%
!!! CAATTTTTGAAATATTGTTG+TGG + chr4.4:15330627-15330646 MS.gene09022:intron 20.0%
! AATTAGTAAAACACATAAGG+AGG - chr4.4:15330371-15330390 None:intergenic 25.0%
! CTGAGTACATAATTTATTCT+CGG + chr4.4:15330900-15330919 MS.gene09022:intron 25.0%
! GAGAAAAATATCACAAAAAG+AGG + chr4.4:15330229-15330248 MS.gene09022:CDS 25.0%
! GTATATCAAACATTTGATCA+CGG - chr4.4:15331110-15331129 None:intergenic 25.0%
! TGAGTACATAATTTATTCTC+GGG + chr4.4:15330901-15330920 MS.gene09022:intron 25.0%
! TGTTGAATGAAATTGAGATT+TGG + chr4.4:15330133-15330152 MS.gene09022:intron 25.0%
!! GTCAAATTTTATGTAGCTTA+TGG + chr4.4:15330002-15330021 MS.gene09022:intron 25.0%
!!! GAAATTGAGATTTGGAATTT+TGG + chr4.4:15330141-15330160 MS.gene09022:intron 25.0%
!!! TTACATTGATTTTCAGCTTA+TGG + chr4.4:15330036-15330055 MS.gene09022:intron 25.0%
AATTAACAATAGTTCAGCTC+TGG - chr4.4:15330524-15330543 None:intergenic 30.0%
ACTGAACCGATAGTTAAAAT+CGG + chr4.4:15330493-15330512 MS.gene09022:intron 30.0%
ATGTTTGATATACCTTCTCA+AGG + chr4.4:15331117-15331136 MS.gene09022:intron 30.0%
CCTAATTCTTGTGATGAATT+GGG + chr4.4:15330414-15330433 MS.gene09022:intron 30.0%
CGAGAATAAATTATGTACTC+AGG - chr4.4:15330902-15330921 None:intergenic 30.0%
GAGCTGAACTATTGTTAATT+CGG + chr4.4:15330524-15330543 MS.gene09022:intron 30.0%
GTAGAATCATGACAAAAACA+AGG - chr4.4:15329873-15329892 None:intergenic 30.0%
TCCTAATTCTTGTGATGAAT+TGG + chr4.4:15330413-15330432 MS.gene09022:intron 30.0%
TGTTAATTCGGATATTTGAG+AGG + chr4.4:15330536-15330555 MS.gene09022:intron 30.0%
TTAACAATTCCCTATGGAAT+TGG + chr4.4:15331080-15331099 MS.gene09022:intron 30.0%
!! ATTGAGATTTGGAATTTTGG+TGG + chr4.4:15330144-15330163 MS.gene09022:intron 30.0%
!! TGTTTTTGTTTGGCCTATTA+GGG - chr4.4:15330766-15330785 None:intergenic 30.0%
!!! TTGTTTTTGTTTGGCCTATT+AGG - chr4.4:15330767-15330786 None:intergenic 30.0%
AAAGGAAATGACTATCCTGT+TGG + chr4.4:15330277-15330296 MS.gene09022:CDS 35.0%
AATCGTATTATAGTGCAGAG+AGG + chr4.4:15331027-15331046 MS.gene09022:intron 35.0%
AGGGATTTAACAATTCCCTA+TGG + chr4.4:15331074-15331093 MS.gene09022:intron 35.0%
ATAAGGAGGACAACATTGAA+AGG - chr4.4:15330357-15330376 None:intergenic 35.0%
ATCGTATTATAGTGCAGAGA+GGG + chr4.4:15331028-15331047 MS.gene09022:intron 35.0%
ATGAACATGCTATGGAAGAA+AGG - chr4.4:15331161-15331180 None:intergenic 35.0%
CCAGAAACAACTGTTAAGAA+AGG + chr4.4:15330259-15330278 MS.gene09022:CDS 35.0%
CCCAATTCATCACAAGAATT+AGG - chr4.4:15330417-15330436 None:intergenic 35.0%
GAATGCAAGAAATAGAAGAG+AGG - chr4.4:15329801-15329820 None:intergenic 35.0%
GATATACCTTCTCAAGGATT+AGG + chr4.4:15331123-15331142 MS.gene09022:intron 35.0%
TAAGGAGGACAACATTGAAA+GGG - chr4.4:15330356-15330375 None:intergenic 35.0%
TATCATCCTAATCCTTGAGA+AGG - chr4.4:15331132-15331151 None:intergenic 35.0%
TCAAGCTGTTGAACTATTTG+AGG + chr4.4:15330581-15330600 MS.gene09022:intron 35.0%
! CCTTTCTTAACAGTTGTTTC+TGG - chr4.4:15330262-15330281 None:intergenic 35.0%
! TTCTTCGTCTTTGTTGTCAT+TGG + chr4.4:15330316-15330335 MS.gene09022:CDS 35.0%
! TTGAACTATTTGAGGTCTTG+TGG + chr4.4:15330589-15330608 MS.gene09022:intron 35.0%
!! GGTTCAATCGTTGTTTTGTT+TGG + chr4.4:15330557-15330576 MS.gene09022:intron 35.0%
!! GTTTTTGTTTGGCCTATTAG+GGG - chr4.4:15330765-15330784 None:intergenic 35.0%
!! TTTTTGTTTGGCCTATTAGG+GGG - chr4.4:15330764-15330783 None:intergenic 35.0%
!!! GCTCAACCGATTTTAACTAT+CGG - chr4.4:15330502-15330521 None:intergenic 35.0%
!!! GTTTTGATTTACCCCCTAAT+AGG + chr4.4:15330750-15330769 MS.gene09022:intron 35.0%
ACGAAGAAACCAAGCAAAAC+GGG - chr4.4:15330304-15330323 None:intergenic 40.0%
ATTATAGTGCAGAGAGGGAA+AGG + chr4.4:15331033-15331052 MS.gene09022:intron 40.0%
CAGCTCTCTTTCAGATAATC+AGG + chr4.4:15331264-15331283 MS.gene09022:intron 40.0%
CATAGCTCATGAACATGCTA+TGG - chr4.4:15331169-15331188 None:intergenic 40.0%
GACGAAGAAACCAAGCAAAA+CGG - chr4.4:15330305-15330324 None:intergenic 40.0%
TTATAGTGCAGAGAGGGAAA+GGG + chr4.4:15331034-15331053 MS.gene09022:intron 40.0%
!! GGTAATGGGTAATGGTCAAA+GGG + chr4.4:15331055-15331074 MS.gene09022:intron 40.0%
ATAATCAGGACAGCAACGAG+TGG + chr4.4:15331278-15331297 MS.gene09022:CDS 45.0%
CGAAGAAACCAAGCAAAACG+GGG - chr4.4:15330303-15330322 None:intergenic 45.0%
TCTCAACCTTCCTAACTGCA+AGG - chr4.4:15330204-15330223 None:intergenic 45.0%
!! AGGGAAAGGGTAATGGGTAA+TGG + chr4.4:15331047-15331066 MS.gene09022:intron 45.0%
!! GGGTAATGGGTAATGGTCAA+AGG + chr4.4:15331054-15331073 MS.gene09022:intron 45.0%
!!! TAAATTTTTGAAATATTTTA+TGG + chr4.4:15330681-15330700 MS.gene09022:intron 5.0%
GCAGAGAGGGAAAGGGTAAT+GGG + chr4.4:15331041-15331060 MS.gene09022:intron 50.0%
TGCAGAGAGGGAAAGGGTAA+TGG + chr4.4:15331040-15331059 MS.gene09022:intron 50.0%
! AAAGCGCCTTGCAGTTAGGA+AGG + chr4.4:15330195-15330214 MS.gene09022:CDS 50.0%
! CACGGAGCACCAATTCCATA+GGG - chr4.4:15331092-15331111 None:intergenic 50.0%
! CATCAAAGCGCCTTGCAGTT+AGG + chr4.4:15330191-15330210 MS.gene09022:CDS 50.0%
! TCACGGAGCACCAATTCCAT+AGG - chr4.4:15331093-15331112 None:intergenic 50.0%
ATCAGGACAGCAACGAGTGG+AGG + chr4.4:15331281-15331300 MS.gene09022:CDS 55.0%
! CGAGTGGAGGCATGGCATAA+TGG + chr4.4:15331294-15331313 MS.gene09022:CDS 55.0%
CCAAGCAAAACGGGGCCAAC+AGG - chr4.4:15330295-15330314 None:intergenic 60.0%
GACAGCAACGAGTGGAGGCA+TGG + chr4.4:15331286-15331305 MS.gene09022:CDS 60.0%
! CCTGTTGGCCCCGTTTTGCT+TGG + chr4.4:15330292-15330311 MS.gene09022:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 15329782 15331324 15329782 ID=MS.gene09022
chr4.4 mRNA 15329782 15331324 15329782 ID=MS.gene09022.t1;Parent=MS.gene09022
chr4.4 exon 15329782 15329784 15329782 ID=MS.gene09022.t1.exon1;Parent=MS.gene09022.t1
chr4.4 CDS 15329782 15329784 15329782 ID=cds.MS.gene09022.t1;Parent=MS.gene09022.t1
chr4.4 exon 15330187 15330343 15330187 ID=MS.gene09022.t1.exon2;Parent=MS.gene09022.t1
chr4.4 CDS 15330187 15330343 15330187 ID=cds.MS.gene09022.t1;Parent=MS.gene09022.t1
chr4.4 exon 15331278 15331324 15331278 ID=MS.gene09022.t1.exon3;Parent=MS.gene09022.t1
chr4.4 CDS 15331278 15331324 15331278 ID=cds.MS.gene09022.t1;Parent=MS.gene09022.t1
Gene Sequence

>MS.gene09022

ATGGTTCGTTTTTTTTCCTCTCTTCTATTTCTTGCATTCTTCAGTTTCGTTAATTTTCACTCTTCGTTTAGATCTATTAGTTTTTTTACCTTGTTTTTGTCATGATTCTACTGAATTTTTTGAAAAATTAATCCGAATTTTTGTTTTGAAATTGAATTGATGCAAATCTCGTTGTATTTATGCAAATTCATAATTGAAATCAGAAATTTATCAAAATTAGGTCAAATTTTATGTAGCTTATGGATCTATGTGTGTTACATTGATTTTCAGCTTATGGATCTGAAATAATTTGTTCTGATCTGATCAATGTGATTTTGAATTGTTAAATTTTGATTGAATTTGGTTTTGTGATGTTGAATGAAATTGAGATTTGGAATTTTGGTGGTTTTTTTTTTGTGTGTGTAGACTACATCAAAGCGCCTTGCAGTTAGGAAGGTTGAGAAGTTCGAGAAAAATATCACAAAAAGAGGAAGTGTGCCAGAAACAACTGTTAAGAAAGGAAATGACTATCCTGTTGGCCCCGTTTTGCTTGGTTTCTTCGTCTTTGTTGTCATTGGATCATGTAAGATTGCCCTTTCAATGTTGTCCTCCTTATGTGTTTTACTAATTTTTGTTTTCATTCGAATCATATTCCTAATTCTTGTGATGAATTGGGAATGTTTATTGTTTGTGTGATTTATTTGGTATGCATTGCTAACTAATGTTTTAAAAACTGAACCGATAGTTAAAATCGGTTGAGCCAGAGCTGAACTATTGTTAATTCGGATATTTGAGAGGTTCAATCGTTGTTTTGTTTGGTTCAAGCTGTTGAACTATTTGAGGTCTTGTGGATTCAATGTCCGATTCAATTTTTGAAATATTGTTGTGGGGTTAACAAGTCTTTACCCTCTGTAATATAGTAAATTTTTGAAATATTTTATGGTTTTCCCCCTGTAATTTTTTTTTATTTACCCCTGTACAAATTTTTTGTTTTGATTTACCCCCTAATAGGCCAAACAAAAACAAAAATTTCTTGAAAAATAAAATATGTTTCATTGCTTGATGAATATGTTTTGTATTCCAATTTCAAACACATTAATATAGTTAACAAGAACATTGCTCATCTGTATAACAAGTACCTGAGTACATAATTTATTCTCGGGCCTTGAATTGAATTGAAAATTATTTATTTAGATTGCAATTCTTGTTGAATCAAATATGCATTCGCAACTAAAATATTCTTTTTGTGTGCGTTTTATATTTAAGAATCGTATTATAGTGCAGAGAGGGAAAGGGTAATGGGTAATGGTCAAAGGGATTTAACAATTCCCTATGGAATTGGTGCTCCGTGATCAAATGTTTGATATACCTTCTCAAGGATTAGGATGATATATTTACCTTTCTTCCATAGCATGTTCATGAGCTATGTTTTGTAGTTTCATTATCATTTTCATAATCTATGTTGTACAGTTTCATCTGACATGTTTGCATGCTTTTATTGTACAGCTCTCTTTCAGATAATCAGGACAGCAACGAGTGGAGGCATGGCATAATGGAGCTTTGA

Protein sequence

>MS.gene09022.t1

MTTSKRLAVRKVEKFEKNITKRGSVPETTVKKGNDYPVGPVLLGFFVFVVIGSYNQDSNEWRHGIMEL