AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene42125


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42125.t1 MTR_6g004810 85.217 115 10 1 5 112 202 316 1.35e-31 116
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 71 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGGTGCATTTGCCTTCTT+TGG 0.267077 7.3:-33269368 None:intergenic
GGTAGGTCTGGCAGTGCTTA+TGG 0.308374 7.3:+33269479 MS.gene42125:CDS
GCTAGTGGAGCTTCCTTCTA+TGG 0.310496 7.3:+33269788 MS.gene42125:CDS
GCTGGTCAATATCATCCTTA+TGG 0.340155 7.3:+33269833 MS.gene42125:CDS
GATGGATTTGGTGGCGGATT+TGG 0.341068 7.3:+33269458 MS.gene42125:CDS
GCCAGCCTTGGCGTTGGATA+TGG 0.345581 7.3:+33269758 MS.gene42125:CDS
TCCACCTCTGCTTCCATAGA+AGG 0.351882 7.3:-33269801 None:intergenic
GGTGGATATGCTGGAGCAAT+GGG 0.361158 7.3:+33269551 MS.gene42125:CDS
GGAGATGCTTATGATGGATT+TGG 0.368480 7.3:+33269446 MS.gene42125:CDS
CTGGCAGTGCTTATGGTGGT+AGG 0.388131 7.3:+33269486 MS.gene42125:CDS
TATGGAAGCAGAGGTGGATA+TGG 0.395763 7.3:+33269806 MS.gene42125:CDS
GGCTATGGAAGTGAGTTTGC+TGG 0.412667 7.3:+33269524 MS.gene42125:CDS
AGGCAACAAACTCTTGCAAA+TGG 0.425471 7.3:-33269105 None:intergenic
CTAGCATATGCAGGTCGTTA+TGG 0.428760 7.3:+33269599 MS.gene42125:CDS
GGCAACAAACTCTTGCAAAT+GGG 0.429927 7.3:-33269104 None:intergenic
CCTAGTGGGTATGGCGGACT+TGG 0.432034 7.3:+33269647 MS.gene42125:CDS
GCTGGGTATGGTGGATATGC+TGG 0.433435 7.3:+33269542 MS.gene42125:CDS
CTTTGAGTCATTGTAGCGCT+TGG 0.451111 7.3:-33269399 None:intergenic
GGAAGTGAGTTTGCTGGGTA+TGG 0.452480 7.3:+33269530 MS.gene42125:CDS
TCCATATCCAACGCCAAGGC+TGG 0.457806 7.3:-33269759 None:intergenic
TGGCAGTGCTTATGGTGGTA+GGG 0.459037 7.3:+33269487 MS.gene42125:CDS
TGGTGGATATGCTGGAGCAA+TGG 0.461072 7.3:+33269550 MS.gene42125:CDS
TCATACATCAGTGGTGGGTA+TGG 0.467506 7.3:+33269425 MS.gene42125:CDS
GGTGGCGGATTTGGTAGGTC+TGG 0.474553 7.3:+33269467 MS.gene42125:CDS
ATATCATCCTTATGGAAGAT+AGG 0.474716 7.3:+33269841 MS.gene42125:CDS
GGCTATGATCCTAGTGGGTA+TGG 0.490193 7.3:+33269638 MS.gene42125:CDS
CAAGAAGGCTGAACCAAAGA+AGG 0.494018 7.3:+33269355 MS.gene42125:CDS
GGTGGTCCGTATGGTGGCTA+TGG 0.495116 7.3:+33269509 MS.gene42125:CDS
GGCAGTGCTTATGGTGGTAG+GGG 0.498411 7.3:+33269488 MS.gene42125:CDS
GGTGGTAGGGGTGGTCCGTA+TGG 0.504580 7.3:+33269500 MS.gene42125:CDS
ATAACTGCCTATCTTCCATA+AGG 0.509101 7.3:-33269848 None:intergenic
AGTGCTTATGGTGGTAGGGG+TGG 0.514243 7.3:+33269491 MS.gene42125:CDS
TCCTTCTATGGAAGCAGAGG+TGG 0.516993 7.3:+33269800 MS.gene42125:CDS
AGTGAGTTTGCTGGGTATGG+TGG 0.521591 7.3:+33269533 MS.gene42125:CDS
TTTGTAGGTGGAAATCAAGA+AGG 0.536506 7.3:+33269340 MS.gene42125:intron
AGTGGCTATGATGCCAGCCT+TGG 0.544416 7.3:+33269746 MS.gene42125:intron
GATTTGGTGGCGGATTTGGT+AGG 0.544659 7.3:+33269462 MS.gene42125:CDS
AGAGGTGGATATGGTGGTGC+TGG 0.552625 7.3:+33269815 MS.gene42125:CDS
TCATTGTAGCGCTTGGATGA+TGG 0.553767 7.3:-33269392 None:intergenic
AGCCTTGGCGTTGGATATGG+AGG 0.558812 7.3:+33269761 MS.gene42125:CDS
GATCCTTCTCTAGCATATGC+AGG 0.559447 7.3:+33269590 MS.gene42125:CDS
GCTATGGAAGTGAGTTTGCT+GGG 0.562673 7.3:+33269525 MS.gene42125:CDS
CGACCTGCATATGCTAGAGA+AGG 0.563559 7.3:-33269593 None:intergenic
GCAACAAACTCTTGCAAATG+GGG 0.563782 7.3:-33269103 None:intergenic
TAGAGAAGGATCTCCTCTGT+AGG 0.567608 7.3:-33269579 None:intergenic
GGTAGGGGTGGTCCGTATGG+TGG 0.570641 7.3:+33269503 MS.gene42125:CDS
TGGATATGCTGGAGCAATGG+GGG 0.572776 7.3:+33269553 MS.gene42125:CDS
GATGCTTATGATGGATTTGG+TGG 0.573529 7.3:+33269449 MS.gene42125:CDS
CCAAGTCCGCCATACCCACT+AGG 0.573852 7.3:-33269647 None:intergenic
GGGTATGGAGATGCTTATGA+TGG 0.574308 7.3:+33269440 MS.gene42125:CDS
AGAGAAGGATCTCCTCTGTA+GGG 0.575562 7.3:-33269578 None:intergenic
AAGTCTTCATACATCAGTGG+TGG 0.577289 7.3:+33269419 MS.gene42125:CDS
GCTTATGATGGATTTGGTGG+CGG 0.594491 7.3:+33269452 MS.gene42125:CDS
GCATATGCAGGTCGTTATGG+TGG 0.601840 7.3:+33269602 MS.gene42125:CDS
GGATATGCTGGAGCAATGGG+GGG 0.602473 7.3:+33269554 MS.gene42125:CDS
TCACTTCCATAGCCACCATA+CGG 0.613738 7.3:-33269515 None:intergenic
TATGATGCCAGCCTTGGCGT+TGG 0.617671 7.3:+33269752 MS.gene42125:CDS
TAGCGCTTGGATGATGGAGG+TGG 0.622852 7.3:-33269386 None:intergenic
GCAGAGGCTATGATCCTAGT+GGG 0.624317 7.3:+33269633 MS.gene42125:CDS
AGTCTTCATACATCAGTGGT+GGG 0.625749 7.3:+33269420 MS.gene42125:CDS
GGATATGGAGGAGCTGCTAG+TGG 0.626427 7.3:+33269773 MS.gene42125:CDS
GCTTCCTTCTATGGAAGCAG+AGG 0.626636 7.3:+33269797 MS.gene42125:CDS
CTCCTCCATATCCAACGCCA+AGG 0.636818 7.3:-33269763 None:intergenic
AGCAGAGGCTATGATCCTAG+TGG 0.638315 7.3:+33269632 MS.gene42125:CDS
GTGGATATGCTGGAGCAATG+GGG 0.652433 7.3:+33269552 MS.gene42125:CDS
TCAAAGTCTTCATACATCAG+TGG 0.669588 7.3:+33269416 MS.gene42125:CDS
GGAAGCAGAGGTGGATATGG+TGG 0.673211 7.3:+33269809 MS.gene42125:CDS
AGGTCTGGCAGTGCTTATGG+TGG 0.677321 7.3:+33269482 MS.gene42125:CDS
TATGGTGGAAGCTTTAGCAG+AGG 0.678683 7.3:+33269617 MS.gene42125:CDS
TTGTAGCGCTTGGATGATGG+AGG 0.684955 7.3:-33269389 None:intergenic
TATGATCCTAGTGGGTATGG+CGG 0.710234 7.3:+33269641 MS.gene42125:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATGTAAAGAAAAAATGA+TGG - chr7.3:33269244-33269263 None:intergenic 15.0%
!! AATAAAACAAAACAAAAATC+TGG - chr7.3:33269270-33269289 None:intergenic 15.0%
!! ATAAAACAAAACAAAAATCT+GGG - chr7.3:33269269-33269288 None:intergenic 15.0%
!! ATTGAATAATGTATTAATGA+AGG - chr7.3:33269128-33269147 None:intergenic 15.0%
!! TAAAAAATGATTGTAACTCA+AGG - chr7.3:33269207-33269226 None:intergenic 20.0%
!! TTCAATTTATCATGTAAATC+TGG + chr7.3:33269142-33269161 MS.gene42125:intron 20.0%
! AATTTATCATGTAAATCTGG+AGG + chr7.3:33269145-33269164 MS.gene42125:intron 25.0%
! TCTTAAATTCAAGTGTTTAC+TGG + chr7.3:33269175-33269194 MS.gene42125:intron 25.0%
!! CTTTGTTATCTTTACTTTGT+AGG + chr7.3:33269325-33269344 MS.gene42125:intron 25.0%
ATATCATCCTTATGGAAGAT+AGG + chr7.3:33269841-33269860 MS.gene42125:CDS 30.0%
TGTTATCTTTACTTTGTAGG+TGG + chr7.3:33269328-33269347 MS.gene42125:intron 30.0%
ATAACTGCCTATCTTCCATA+AGG - chr7.3:33269851-33269870 None:intergenic 35.0%
TCAAAGTCTTCATACATCAG+TGG + chr7.3:33269416-33269435 MS.gene42125:CDS 35.0%
TTTGTAGGTGGAAATCAAGA+AGG + chr7.3:33269340-33269359 MS.gene42125:intron 35.0%
AAGTCTTCATACATCAGTGG+TGG + chr7.3:33269419-33269438 MS.gene42125:CDS 40.0%
AGGCAACAAACTCTTGCAAA+TGG - chr7.3:33269108-33269127 None:intergenic 40.0%
AGTCTTCATACATCAGTGGT+GGG + chr7.3:33269420-33269439 MS.gene42125:CDS 40.0%
GCAACAAACTCTTGCAAATG+GGG - chr7.3:33269106-33269125 None:intergenic 40.0%
GCTGGTCAATATCATCCTTA+TGG + chr7.3:33269833-33269852 MS.gene42125:CDS 40.0%
GGCAACAAACTCTTGCAAAT+GGG - chr7.3:33269107-33269126 None:intergenic 40.0%
!! GATGCTTATGATGGATTTGG+TGG + chr7.3:33269449-33269468 MS.gene42125:CDS 40.0%
!! GGAGATGCTTATGATGGATT+TGG + chr7.3:33269446-33269465 MS.gene42125:CDS 40.0%
AGAGAAGGATCTCCTCTGTA+GGG - chr7.3:33269581-33269600 None:intergenic 45.0%
CAAGAAGGCTGAACCAAAGA+AGG + chr7.3:33269355-33269374 MS.gene42125:CDS 45.0%
CTAGCATATGCAGGTCGTTA+TGG + chr7.3:33269599-33269618 MS.gene42125:CDS 45.0%
CTTTGAGTCATTGTAGCGCT+TGG - chr7.3:33269402-33269421 None:intergenic 45.0%
GATCCTTCTCTAGCATATGC+AGG + chr7.3:33269590-33269609 MS.gene42125:CDS 45.0%
GGGTATGGAGATGCTTATGA+TGG + chr7.3:33269440-33269459 MS.gene42125:CDS 45.0%
TAGAGAAGGATCTCCTCTGT+AGG - chr7.3:33269582-33269601 None:intergenic 45.0%
TATGGAAGCAGAGGTGGATA+TGG + chr7.3:33269806-33269825 MS.gene42125:CDS 45.0%
TCACTTCCATAGCCACCATA+CGG - chr7.3:33269518-33269537 None:intergenic 45.0%
TCATTGTAGCGCTTGGATGA+TGG - chr7.3:33269395-33269414 None:intergenic 45.0%
! GCTATGGAAGTGAGTTTGCT+GGG + chr7.3:33269525-33269544 MS.gene42125:CDS 45.0%
! TATGATCCTAGTGGGTATGG+CGG + chr7.3:33269641-33269660 MS.gene42125:CDS 45.0%
! TATGGTGGAAGCTTTAGCAG+AGG + chr7.3:33269617-33269636 MS.gene42125:CDS 45.0%
!! GCTTATGATGGATTTGGTGG+CGG + chr7.3:33269452-33269471 MS.gene42125:CDS 45.0%
!! TCATACATCAGTGGTGGGTA+TGG + chr7.3:33269425-33269444 MS.gene42125:CDS 45.0%
AGCAGAGGCTATGATCCTAG+TGG + chr7.3:33269632-33269651 MS.gene42125:CDS 50.0%
CGACCTGCATATGCTAGAGA+AGG - chr7.3:33269596-33269615 None:intergenic 50.0%
GCAGAGGCTATGATCCTAGT+GGG + chr7.3:33269633-33269652 MS.gene42125:CDS 50.0%
GCATATGCAGGTCGTTATGG+TGG + chr7.3:33269602-33269621 MS.gene42125:CDS 50.0%
GCTAGTGGAGCTTCCTTCTA+TGG + chr7.3:33269788-33269807 MS.gene42125:CDS 50.0%
GCTTCCTTCTATGGAAGCAG+AGG + chr7.3:33269797-33269816 MS.gene42125:CDS 50.0%
GGCTATGGAAGTGAGTTTGC+TGG + chr7.3:33269524-33269543 MS.gene42125:CDS 50.0%
GGTGGATATGCTGGAGCAAT+GGG + chr7.3:33269551-33269570 MS.gene42125:CDS 50.0%
GGTGGTGCATTTGCCTTCTT+TGG - chr7.3:33269371-33269390 None:intergenic 50.0%
GTGGATATGCTGGAGCAATG+GGG + chr7.3:33269552-33269571 MS.gene42125:CDS 50.0%
TCCACCTCTGCTTCCATAGA+AGG - chr7.3:33269804-33269823 None:intergenic 50.0%
TCCTTCTATGGAAGCAGAGG+TGG + chr7.3:33269800-33269819 MS.gene42125:CDS 50.0%
TGGATATGCTGGAGCAATGG+GGG + chr7.3:33269553-33269572 MS.gene42125:CDS 50.0%
TGGCAGTGCTTATGGTGGTA+GGG + chr7.3:33269487-33269506 MS.gene42125:CDS 50.0%
! GAGAGTTATGGGGCATTTGG+TGG + chr7.3:33269671-33269690 MS.gene42125:intron 50.0%
! GGCTATGATCCTAGTGGGTA+TGG + chr7.3:33269638-33269657 MS.gene42125:CDS 50.0%
! GGTGAGAGTTATGGGGCATT+TGG + chr7.3:33269668-33269687 MS.gene42125:intron 50.0%
! TGGTGGATATGCTGGAGCAA+TGG + chr7.3:33269550-33269569 MS.gene42125:CDS 50.0%
! TTGTAGCGCTTGGATGATGG+AGG - chr7.3:33269392-33269411 None:intergenic 50.0%
!! AGTGAGTTTGCTGGGTATGG+TGG + chr7.3:33269533-33269552 MS.gene42125:CDS 50.0%
!! CGGACTTGGTGAGAGTTATG+GGG + chr7.3:33269661-33269680 MS.gene42125:intron 50.0%
!! GATGGATTTGGTGGCGGATT+TGG + chr7.3:33269458-33269477 MS.gene42125:CDS 50.0%
!! GATTTGGTGGCGGATTTGGT+AGG + chr7.3:33269462-33269481 MS.gene42125:CDS 50.0%
!! GCGGACTTGGTGAGAGTTAT+GGG + chr7.3:33269660-33269679 MS.gene42125:intron 50.0%
!! GGAAGTGAGTTTGCTGGGTA+TGG + chr7.3:33269530-33269549 MS.gene42125:CDS 50.0%
!! GGTGCTGGTGCATATCAAAG+TGG + chr7.3:33269728-33269747 MS.gene42125:intron 50.0%
AGGTCTGGCAGTGCTTATGG+TGG + chr7.3:33269482-33269501 MS.gene42125:CDS 55.0%
AGTGCTTATGGTGGTAGGGG+TGG + chr7.3:33269491-33269510 MS.gene42125:CDS 55.0%
AGTGGCTATGATGCCAGCCT+TGG + chr7.3:33269746-33269765 MS.gene42125:intron 55.0%
CTCCTCCATATCCAACGCCA+AGG - chr7.3:33269766-33269785 None:intergenic 55.0%
CTGGCAGTGCTTATGGTGGT+AGG + chr7.3:33269486-33269505 MS.gene42125:CDS 55.0%
GGAAGCAGAGGTGGATATGG+TGG + chr7.3:33269809-33269828 MS.gene42125:CDS 55.0%
GGATATGCTGGAGCAATGGG+GGG + chr7.3:33269554-33269573 MS.gene42125:CDS 55.0%
GGATATGGAGGAGCTGCTAG+TGG + chr7.3:33269773-33269792 MS.gene42125:CDS 55.0%
GGCAGTGCTTATGGTGGTAG+GGG + chr7.3:33269488-33269507 MS.gene42125:CDS 55.0%
GGTAGGTCTGGCAGTGCTTA+TGG + chr7.3:33269479-33269498 MS.gene42125:CDS 55.0%
TATGATGCCAGCCTTGGCGT+TGG + chr7.3:33269752-33269771 MS.gene42125:CDS 55.0%
TCCATATCCAACGCCAAGGC+TGG - chr7.3:33269762-33269781 None:intergenic 55.0%
! AGAGGTGGATATGGTGGTGC+TGG + chr7.3:33269815-33269834 MS.gene42125:CDS 55.0%
! AGCCTTGGCGTTGGATATGG+AGG + chr7.3:33269761-33269780 MS.gene42125:CDS 55.0%
!! GCTGGGTATGGTGGATATGC+TGG + chr7.3:33269542-33269561 MS.gene42125:CDS 55.0%
!! GGCGGACTTGGTGAGAGTTA+TGG + chr7.3:33269659-33269678 MS.gene42125:intron 55.0%
!! TAGCGCTTGGATGATGGAGG+TGG - chr7.3:33269389-33269408 None:intergenic 55.0%
!! TATGGGGCATTTGGTGGTGC+AGG + chr7.3:33269677-33269696 MS.gene42125:intron 55.0%
AGAAGAACCACCTCCGCCAG+CGG - chr7.3:33269708-33269727 None:intergenic 60.0%
CCAAGTCCGCCATACCCACT+AGG - chr7.3:33269650-33269669 None:intergenic 60.0%
GGAGGTGGTTCTTCTGGTGC+TGG + chr7.3:33269713-33269732 MS.gene42125:intron 60.0%
GGTGGTCCGTATGGTGGCTA+TGG + chr7.3:33269509-33269528 MS.gene42125:CDS 60.0%
! GCCAGCCTTGGCGTTGGATA+TGG + chr7.3:33269758-33269777 MS.gene42125:CDS 60.0%
!! CCTAGTGGGTATGGCGGACT+TGG + chr7.3:33269647-33269666 MS.gene42125:CDS 60.0%
!! GGTGGCGGATTTGGTAGGTC+TGG + chr7.3:33269467-33269486 MS.gene42125:CDS 60.0%
GCAATGGGGGGACCCTACAG+AGG + chr7.3:33269566-33269585 MS.gene42125:CDS 65.0%
GCTGGCGGAGGTGGTTCTTC+TGG + chr7.3:33269707-33269726 MS.gene42125:intron 65.0%
GGTAGGGGTGGTCCGTATGG+TGG + chr7.3:33269503-33269522 MS.gene42125:CDS 65.0%
GGTGGTAGGGGTGGTCCGTA+TGG + chr7.3:33269500-33269519 MS.gene42125:CDS 65.0%
! GGTGCAGGTGCTGCCGCTGG+CGG + chr7.3:33269692-33269711 MS.gene42125:intron 75.0%
! GGTGGTGCAGGTGCTGCCGC+TGG + chr7.3:33269689-33269708 MS.gene42125:intron 75.0%
!! GCAGGTGCTGCCGCTGGCGG+AGG + chr7.3:33269695-33269714 MS.gene42125:intron 80.0%
!! GGTGCTGCCGCTGGCGGAGG+TGG + chr7.3:33269698-33269717 MS.gene42125:intron 80.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 33269099 33269875 33269099 ID=MS.gene42125
chr7.3 mRNA 33269099 33269875 33269099 ID=MS.gene42125.t1;Parent=MS.gene42125
chr7.3 exon 33269099 33269116 33269099 ID=MS.gene42125.t1.exon1;Parent=MS.gene42125.t1
chr7.3 CDS 33269099 33269116 33269099 ID=cds.MS.gene42125.t1;Parent=MS.gene42125.t1
chr7.3 exon 33269347 33269668 33269347 ID=MS.gene42125.t1.exon2;Parent=MS.gene42125.t1
chr7.3 CDS 33269347 33269668 33269347 ID=cds.MS.gene42125.t1;Parent=MS.gene42125.t1
chr7.3 exon 33269748 33269875 33269748 ID=MS.gene42125.t1.exon3;Parent=MS.gene42125.t1
chr7.3 CDS 33269748 33269875 33269748 ID=cds.MS.gene42125.t1;Parent=MS.gene42125.t1
Gene Sequence

>MS.gene42125

ATGACCCCATTTGCAAGAGTTTGTTGCCTTCATTAATACATTATTCAATTTATCATGTAAATCTGGAGGAAGTTTTTCTTAAATTCAAGTGTTTACTGGTTTGTACCTTGAGTTACAATCATTTTTTACATCATGCGTCGTTCCATCATTTTTTCTTTACATTTTTTCCCAGATTTTTGTTTTGTTTTATTTTCTTCATTTAATGCAATTGTCGTCAATGCATTAACTTTGTTATCTTTACTTTGTAGGTGGAAATCAAGAAGGCTGAACCAAAGAAGGCAAATGCACCACCTCCATCATCCAAGCGCTACAATGACTCAAAGTCTTCATACATCAGTGGTGGGTATGGAGATGCTTATGATGGATTTGGTGGCGGATTTGGTAGGTCTGGCAGTGCTTATGGTGGTAGGGGTGGTCCGTATGGTGGCTATGGAAGTGAGTTTGCTGGGTATGGTGGATATGCTGGAGCAATGGGGGGACCCTACAGAGGAGATCCTTCTCTAGCATATGCAGGTCGTTATGGTGGAAGCTTTAGCAGAGGCTATGATCCTAGTGGGTATGGCGGACTTGGTGAGAGTTATGGGGCATTTGGTGGTGCAGGTGCTGCCGCTGGCGGAGGTGGTTCTTCTGGTGCTGGTGCATATCAAAGTGGCTATGATGCCAGCCTTGGCGTTGGATATGGAGGAGCTGCTAGTGGAGCTTCCTTCTATGGAAGCAGAGGTGGATATGGTGGTGCTGGTCAATATCATCCTTATGGAAGATAGGCAGTTATACTAA

Protein sequence

>MS.gene42125.t1

MTPFARVEIKKAEPKKANAPPPSSKRYNDSKSSYISGGYGDAYDGFGGGFGRSGSAYGGRGGPYGGYGSEFAGYGGYAGAMGGPYRGDPSLAYAGRYGGSFSRGYDPSGYGGLVAMMPALALDMEELLVELPSMEAEVDMVVLVNIILMEDRQLY