AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43133

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43133.t1 MTR_7g021850 93.787 338 20 1 1 338 1 337 0.0 657
MS.gene43133.t1 MTR_7g021680 89.706 340 34 1 1 340 1 339 0.0 641
MS.gene43133.t1 MTR_7g021820 89.706 340 34 1 1 340 1 339 0.0 641
MS.gene43133.t1 MTR_1g102750 59.821 336 128 5 4 333 5 339 8.46e-140 400
MS.gene43133.t1 MTR_7g114980 58.160 337 131 5 6 333 13 348 9.87e-138 395
MS.gene43133.t1 MTR_7g114990 57.530 332 133 5 7 333 16 344 1.80e-137 394
MS.gene43133.t1 MTR_7g114970 55.917 338 142 5 4 335 7 343 3.04e-131 378
MS.gene43133.t1 MTR_7g115010 53.433 335 133 5 6 333 11 329 3.65e-126 365
MS.gene43133.t1 MTR_1g102750 62.411 282 103 3 4 283 5 285 8.87e-124 358
MS.gene43133.t1 MTR_7g114980 61.210 281 103 3 6 281 13 292 1.32e-120 349
MS.gene43133.t1 MTR_7g114970 60.714 280 107 3 4 281 7 285 2.39e-120 348
MS.gene43133.t1 MTR_7g114970 60.573 279 107 3 4 280 7 284 1.97e-119 346
MS.gene43133.t1 MTR_7g021825 89.726 146 15 0 158 303 15 160 6.71e-98 286
MS.gene43133.t1 MTR_7g114970 57.895 247 98 4 94 335 4 249 5.86e-96 285
MS.gene43133.t1 MTR_7g114970 63.402 194 70 1 6 198 9 202 1.49e-85 257
MS.gene43133.t1 MTR_7g114970 57.286 199 79 4 139 332 1 198 8.72e-74 227
MS.gene43133.t1 MTR_7g021670 83.206 131 21 1 1 131 1 130 5.51e-73 226
MS.gene43133.t1 MTR_7g021670 87.963 108 13 0 232 339 145 252 1.81e-64 205
MS.gene43133.t1 MTR_7g021685 86.957 69 9 0 1 69 1 69 9.51e-39 134
MS.gene43133.t1 MTR_3g449790 29.885 348 188 11 1 315 32 356 8.71e-32 122
MS.gene43133.t1 MTR_3g449790 29.310 348 184 11 1 315 32 350 2.21e-31 121
MS.gene43133.t1 MTR_1g102740 51.852 108 42 2 233 333 3 107 2.75e-31 114
MS.gene43133.t1 MTR_1g042730 27.143 350 201 14 8 310 65 407 4.41e-23 99.4
MS.gene43133.t1 MTR_3g092140 27.609 297 178 11 36 311 69 349 9.76e-23 99.4
MS.gene43133.t1 MTR_1g047250 27.077 325 206 12 9 313 6 319 4.33e-20 89.7
MS.gene43133.t1 MTR_4g092750 31.019 216 135 7 8 214 5 215 4.45e-17 80.9
MS.gene43133.t1 MTR_4g092750 31.019 216 135 7 8 214 5 215 5.36e-17 80.9
MS.gene43133.t1 MTR_4g092750 31.019 216 135 7 8 214 5 215 1.01e-16 79.7
MS.gene43133.t1 MTR_8g088160 25.735 272 151 11 14 239 49 315 2.43e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43133.t1 AT1G60710 77.811 338 73 1 4 339 7 344 0.0 552
MS.gene43133.t1 AT1G60730 77.811 338 73 1 4 339 7 344 0.0 549
MS.gene43133.t1 AT1G60690 76.401 339 78 1 4 340 7 345 0.0 548
MS.gene43133.t1 AT1G60680 75.811 339 79 2 4 339 7 345 0.0 539
MS.gene43133.t1 AT1G60730 73.464 358 73 2 4 339 7 364 0.0 536
MS.gene43133.t1 AT1G10810 71.765 340 94 1 2 339 5 344 0.0 511
MS.gene43133.t1 AT1G60750 72.566 339 73 3 4 339 8 329 1.50e-179 500
MS.gene43133.t1 AT1G10810 71.956 271 74 1 71 339 22 292 2.58e-145 412
MS.gene43133.t1 AT1G60680 79.386 228 46 1 4 230 7 234 4.98e-132 376
MS.gene43133.t1 AT1G10810 73.932 234 61 0 2 235 5 238 4.29e-124 357
MS.gene43133.t1 AT1G60730 76.259 139 33 0 4 142 7 145 5.27e-73 224
MS.gene43133.t1 AT1G06690 29.819 332 199 11 6 315 48 367 1.05e-26 108
MS.gene43133.t1 AT5G53580 30.000 290 160 11 46 311 80 350 9.88e-26 106
MS.gene43133.t1 AT1G04690 27.987 318 180 11 8 297 5 301 1.49e-24 102
MS.gene43133.t1 AT1G04420 26.479 355 207 14 8 315 60 407 3.82e-23 99.8
MS.gene43133.t1 AT4G33670 26.829 328 207 10 1 315 1 308 6.00e-23 97.8
MS.gene43133.t1 AT2G37790 25.723 311 166 14 43 328 35 305 1.62e-12 67.8

Find 78 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATTTCCCTTCAATATATT+TGG 0.148190 7.2:-77516342 None:intergenic
TCCAATTGCACAGCTGTTAT+TGG 0.154954 7.2:-77516997 None:intergenic
TTGATTAAAGTTCTCCATTT+TGG 0.183974 7.2:-77517995 None:intergenic
AAGAAATAATTCCAACTTGC+AGG 0.276199 7.2:+77517049 MS.gene43133:CDS
ATACTCCACCACTCTCTTCT+TGG 0.280266 7.2:+77518136 MS.gene43133:CDS
CTTGATACCTCTGACATCTA+TGG 0.288925 7.2:+77516059 MS.gene43133:CDS
GTTGAATTGGCTACAAAGTT+TGG 0.298080 7.2:+77516317 MS.gene43133:CDS
GAAGGGAAATTTGAGATCTT+TGG 0.300695 7.2:+77516353 MS.gene43133:CDS
AGTGAGAGAGAAGGTTGAAT+TGG 0.360017 7.2:+77516304 MS.gene43133:CDS
GATACCTGGAACCACCAAAA+TGG 0.362093 7.2:+77517981 MS.gene43133:CDS
TCCAATAACAGCTGTGCAAT+TGG 0.363190 7.2:+77516996 MS.gene43133:CDS
TTTCTTAGGGAACTTGGTAT+TGG 0.374176 7.2:+77517325 MS.gene43133:intron
GCTGTCAAGGGTGGTAGATA+TGG 0.391549 7.2:+77518084 MS.gene43133:CDS
ATTTGAGAGGGTTAATGAAC+TGG 0.426318 7.2:+77517885 MS.gene43133:CDS
CTGTCAAGGGTGGTAGATAT+GGG 0.429429 7.2:+77518085 MS.gene43133:CDS
TAAACTAACACAAGAAGAAA+TGG 0.436815 7.2:+77518038 MS.gene43133:CDS
AGAGCAATCATGTCAGGTTC+AGG 0.437605 7.2:-77516002 None:intergenic
GCAGTAGGAAGAATGAAGTT+AGG 0.442452 7.2:+77515906 MS.gene43133:CDS
AAAAGTGACACCAGATTGTA+TGG 0.442868 7.2:-77516036 None:intergenic
ATATAGTCTGATTCTGCTGC+AGG 0.450000 7.2:-77517864 None:intergenic
CAAGCTTCTCTCACGTAAGC+TGG 0.451335 7.2:-77516380 None:intergenic
CTTAAGAAACTTGTTGAAGA+AGG 0.453505 7.2:+77516907 MS.gene43133:CDS
CACACCAACGAACTCCTCCT+TGG 0.466667 7.2:+77516086 MS.gene43133:CDS
ATTGTTGCATATAGTCCTCT+TGG 0.471189 7.2:+77517349 MS.gene43133:CDS
AATTGTGGACAGGCTTTAAG+AGG 0.474129 7.2:+77516278 MS.gene43133:intron
TTTGGAGCCAAATATATTGA+AGG 0.475808 7.2:+77516335 MS.gene43133:CDS
TAATGAACTGGCTACAAAGA+AGG 0.479658 7.2:+77517897 MS.gene43133:CDS
TCGGGCACACGTCGTTTCCT+TGG 0.481328 7.2:-77517960 None:intergenic
ATTCACCATGCCATACAATC+TGG 0.483546 7.2:+77516026 MS.gene43133:CDS
AAGAGGAGGAGTGAGAGAGA+AGG 0.485439 7.2:+77516295 MS.gene43133:CDS
ATGAGTATGTCAGGTGTCTA+TGG 0.492539 7.2:+77515969 MS.gene43133:CDS
AATCAAACCGTGATTTCAAT+CGG 0.493629 7.2:-77516476 None:intergenic
CGTCTCAGCTTGCATTGGCA+TGG 0.497948 7.2:+77517929 MS.gene43133:CDS
TGCAATTGGAGTGGTCACTA+TGG 0.500317 7.2:+77517010 MS.gene43133:CDS
CTTGGTTGCATGAGTATGTC+AGG 0.501159 7.2:+77515960 MS.gene43133:CDS
ATCATGTCAGGTTCAGGCTT+AGG 0.505151 7.2:-77515996 None:intergenic
GAAGTGTCCTTGCAAGGACT+TGG 0.508887 7.2:+77515942 MS.gene43133:CDS
GTCTTTAGCAGATGCTGTCA+AGG 0.511022 7.2:+77518071 MS.gene43133:CDS
AACGACGTGTGCCCGATACC+TGG 0.512377 7.2:+77517967 MS.gene43133:CDS
TCGTCTTCCGATTGAAATCA+CGG 0.531137 7.2:+77516469 MS.gene43133:CDS
GAAGTTAGGATCACAAGGAA+TGG 0.532873 7.2:+77515920 MS.gene43133:CDS
TCTTCTGATTGTTGAAGCTG+AGG 0.532875 7.2:-77516959 None:intergenic
ACTAAATCAAACCTGCAAGT+TGG 0.537019 7.2:-77517060 None:intergenic
AGAAGAACAATGACAGCAGT+AGG 0.537429 7.2:+77515891 None:intergenic
TACTCCGTCTCAGCTTGCAT+TGG 0.539631 7.2:+77517924 MS.gene43133:CDS
GGAGGAGTTCGTTGGTGTGA+GGG 0.544146 7.2:-77516082 None:intergenic
GAGAACTTTAATCAAAACAT+TGG 0.545978 7.2:+77518003 MS.gene43133:CDS
AATGAACTGGCTACAAAGAA+GGG 0.546567 7.2:+77517898 MS.gene43133:CDS
CATGCCAATGCAAGCTGAGA+CGG 0.554180 7.2:-77517928 None:intergenic
ATGTCAGGTTCAGGCTTAGG+AGG 0.557823 7.2:-77515993 None:intergenic
TGAATGAGAGCAATCATGTC+AGG 0.558885 7.2:-77516008 None:intergenic
CTTTCCAAGGAGGAGTTCGT+TGG 0.559199 7.2:-77516090 None:intergenic
AACAAAGGATGACTACCGCC+AGG 0.563602 7.2:+77517414 MS.gene43133:CDS
TTGGAGCCAAATATATTGAA+GGG 0.564347 7.2:+77516336 MS.gene43133:CDS
AGGAGGAGTTCGTTGGTGTG+AGG 0.567455 7.2:-77516083 None:intergenic
GCATATAGTCCTCTTGGACG+AGG 0.577164 7.2:+77517355 MS.gene43133:CDS
CATGCAACCAAGTCCTTGCA+AGG 0.584248 7.2:-77515949 None:intergenic
AGAATCAGACTATATTTGAG+AGG 0.585843 7.2:+77517872 MS.gene43133:CDS
AGAAAGTAAGAACCTTTCCA+AGG 0.586920 7.2:-77516103 None:intergenic
GGAATGGAAGTGTCCTTGCA+AGG 0.592055 7.2:+77515936 MS.gene43133:CDS
GAATCAGACTATATTTGAGA+GGG 0.604511 7.2:+77517873 MS.gene43133:CDS
GAAAAGAATCCTCGTCCAAG+AGG 0.611487 7.2:-77517364 None:intergenic
CAACGAACTCCTCCTTGGAA+AGG 0.612819 7.2:+77516091 MS.gene43133:CDS
ACTATGGTCAAGAGATGTTG+AGG 0.614034 7.2:+77517026 MS.gene43133:CDS
TGTGGACAGGCTTTAAGAGG+AGG 0.614236 7.2:+77516281 MS.gene43133:intron
ATGGGGATGAAATAAGTACA+TGG 0.614624 7.2:+77518103 MS.gene43133:CDS
TCTTTAGCAGATGCTGTCAA+GGG 0.626442 7.2:+77518072 MS.gene43133:CDS
GATTGTTGAGAACTTAACAA+AGG 0.637901 7.2:+77517399 MS.gene43133:CDS
AGAATGAAGTTAGGATCACA+AGG 0.639560 7.2:+77515915 MS.gene43133:CDS
TTAGCAGATGCTGTCAAGGG+TGG 0.645754 7.2:+77518075 MS.gene43133:CDS
GCTTTCCAAGAAGAGAGTGG+TGG 0.651055 7.2:-77518141 None:intergenic
TAACAGCTGTGCAATTGGAG+TGG 0.661184 7.2:+77517001 MS.gene43133:CDS
AAGTAAGAACCTTTCCAAGG+AGG 0.662248 7.2:-77516100 None:intergenic
ACAGCTTTCCAAGAAGAGAG+TGG 0.666396 7.2:-77518144 None:intergenic
GTGAGGGCCATAGATGTCAG+AGG 0.676572 7.2:-77516066 None:intergenic
TTGGCATGGCTTCATCACCA+AGG 0.680145 7.2:+77517943 MS.gene43133:CDS
TGTCAAGGGTGGTAGATATG+GGG 0.702694 7.2:+77518086 MS.gene43133:CDS
TGACACCAGATTGTATGGCA+TGG 0.709987 7.2:-77516031 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTAAAATAGAACATTTT+GGG - chr7.2:77517727-77517746 None:intergenic 10.0%
!!! TTAATTAAAATAGAACATTT+TGG - chr7.2:77517728-77517747 None:intergenic 10.0%
!!! TTATGATTTTTTTTTTCTTA+GGG + chr7.2:77517312-77517331 MS.gene43133:intron 10.0%
!! ACAAATAGAAGATTATATTT+AGG - chr7.2:77517806-77517825 None:intergenic 15.0%
!! AGATATTTAATAATTGATGT+AGG - chr7.2:77517752-77517771 None:intergenic 15.0%
!! GATATTTAATAATTGATGTA+GGG - chr7.2:77517751-77517770 None:intergenic 15.0%
!! TATAAAAATGTGTTTCAATA+TGG - chr7.2:77516588-77516607 None:intergenic 15.0%
!! TATTACCTAAAATTAATGTT+TGG + chr7.2:77516610-77516629 MS.gene43133:intron 15.0%
!! TATTTGTTTACATGTATAAT+TGG + chr7.2:77516833-77516852 MS.gene43133:intron 15.0%
!!! GTTATGATTTTTTTTTTCTT+AGG + chr7.2:77517311-77517330 MS.gene43133:intron 15.0%
!!! TGGATAAATTATTTTCAAAA+AGG + chr7.2:77516630-77516649 MS.gene43133:intron 15.0%
!!! TTTATCCAAACATTAATTTT+AGG - chr7.2:77516618-77516637 None:intergenic 15.0%
!! AAAAAAAATCATAACTCATG+AGG - chr7.2:77517306-77517325 None:intergenic 20.0%
!! AAATTTCCCTTCAATATATT+TGG - chr7.2:77516345-77516364 None:intergenic 20.0%
!! ACGATTGTAATTTAAGAAAA+TGG + chr7.2:77517169-77517188 MS.gene43133:intron 20.0%
!! GAAGGAAAAATAAAATACAT+TGG + chr7.2:77516925-77516944 MS.gene43133:CDS 20.0%
!! TTAAAATTGCAATGATATGA+AGG + chr7.2:77517132-77517151 MS.gene43133:intron 20.0%
!!! AAAATTCTTTGATTCGATTT+TGG + chr7.2:77516505-77516524 MS.gene43133:intron 20.0%
! AACTTTCTCTCTTAATCTAA+CGG - chr7.2:77516690-77516709 None:intergenic 25.0%
! AGCAAACATATGCTTATTTA+TGG - chr7.2:77516547-77516566 None:intergenic 25.0%
! GAGAACTTTAATCAAAACAT+TGG + chr7.2:77518003-77518022 MS.gene43133:CDS 25.0%
! TAAACTAACACAAGAAGAAA+TGG + chr7.2:77518038-77518057 MS.gene43133:CDS 25.0%
! TATTAATGATGTCTATGTGT+CGG - chr7.2:77517560-77517579 None:intergenic 25.0%
! TTGAATGTACTTACAAGTAT+TGG + chr7.2:77517198-77517217 MS.gene43133:intron 25.0%
!! TTGATTAAAGTTCTCCATTT+TGG - chr7.2:77517998-77518017 None:intergenic 25.0%
!!! TTTTTTTTTCTTAGGGAACT+TGG + chr7.2:77517319-77517338 MS.gene43133:intron 25.0%
AACTGACAAGTATGAAATAC+TGG + chr7.2:77517502-77517521 MS.gene43133:intron 30.0%
AAGAAATAATTCCAACTTGC+AGG + chr7.2:77517049-77517068 MS.gene43133:CDS 30.0%
AATCAAACCGTGATTTCAAT+CGG - chr7.2:77516479-77516498 None:intergenic 30.0%
AATGTGTATGTGTGAAATTG+TGG + chr7.2:77516263-77516282 MS.gene43133:intron 30.0%
AGAATCAGACTATATTTGAG+AGG + chr7.2:77517872-77517891 MS.gene43133:CDS 30.0%
CCCAATCTTACATAGTAAAA+GGG - chr7.2:77516884-77516903 None:intergenic 30.0%
GAATCAGACTATATTTGAGA+GGG + chr7.2:77517873-77517892 MS.gene43133:CDS 30.0%
GATTGTTGAGAACTTAACAA+AGG + chr7.2:77517399-77517418 MS.gene43133:CDS 30.0%
TGTCATATAGATTCTAACCT+TGG + chr7.2:77517698-77517717 MS.gene43133:intron 30.0%
TTGGAGCCAAATATATTGAA+GGG + chr7.2:77516336-77516355 MS.gene43133:CDS 30.0%
! ATTAAAGTTCTCCATTTTGG+TGG - chr7.2:77517995-77518014 None:intergenic 30.0%
! CCCTTTTACTATGTAAGATT+GGG + chr7.2:77516881-77516900 MS.gene43133:intron 30.0%
! CCTTTTACTATGTAAGATTG+GGG + chr7.2:77516882-77516901 MS.gene43133:intron 30.0%
! TCCCTTTTACTATGTAAGAT+TGG + chr7.2:77516880-77516899 MS.gene43133:intron 30.0%
! TTTGGAGCCAAATATATTGA+AGG + chr7.2:77516335-77516354 MS.gene43133:CDS 30.0%
!! CTTAAGAAACTTGTTGAAGA+AGG + chr7.2:77516907-77516926 MS.gene43133:CDS 30.0%
AAAAGTGACACCAGATTGTA+TGG - chr7.2:77516039-77516058 None:intergenic 35.0%
AAACACACGTCTTTAATCGT+TGG + chr7.2:77517257-77517276 MS.gene43133:intron 35.0%
AACGATTAAAGACGTGTGTT+TGG - chr7.2:77517258-77517277 None:intergenic 35.0%
AATGAACTGGCTACAAAGAA+GGG + chr7.2:77517898-77517917 MS.gene43133:CDS 35.0%
ACTAAATCAAACCTGCAAGT+TGG - chr7.2:77517063-77517082 None:intergenic 35.0%
AGAAAGTAAGAACCTTTCCA+AGG - chr7.2:77516106-77516125 None:intergenic 35.0%
AGAATGAAGTTAGGATCACA+AGG + chr7.2:77515915-77515934 MS.gene43133:CDS 35.0%
ATGAAATACTGGCACAAACA+TGG + chr7.2:77517513-77517532 MS.gene43133:intron 35.0%
ATGGGGATGAAATAAGTACA+TGG + chr7.2:77518103-77518122 MS.gene43133:CDS 35.0%
ATTGTTGCATATAGTCCTCT+TGG + chr7.2:77517349-77517368 MS.gene43133:CDS 35.0%
ATTTGAGAGGGTTAATGAAC+TGG + chr7.2:77517885-77517904 MS.gene43133:CDS 35.0%
CCCCAATCTTACATAGTAAA+AGG - chr7.2:77516885-77516904 None:intergenic 35.0%
GTTGAATTGGCTACAAAGTT+TGG + chr7.2:77516317-77516336 MS.gene43133:CDS 35.0%
TAATGAACTGGCTACAAAGA+AGG + chr7.2:77517897-77517916 MS.gene43133:CDS 35.0%
TCACATGTTCAGTATTGTGT+TGG + chr7.2:77517620-77517639 MS.gene43133:intron 35.0%
TTATCTAGCATTTCTCTCTG+TGG + chr7.2:77517102-77517121 MS.gene43133:intron 35.0%
TTTCATACTTGTCAGTTGAG+TGG - chr7.2:77517499-77517518 None:intergenic 35.0%
TTTCTTAGGGAACTTGGTAT+TGG + chr7.2:77517325-77517344 MS.gene43133:intron 35.0%
! ACACGAAACCTTCAAGTTTA+AGG - chr7.2:77517681-77517700 None:intergenic 35.0%
! GAAGGGAAATTTGAGATCTT+TGG + chr7.2:77516353-77516372 MS.gene43133:CDS 35.0%
!!! AGGTTTTCAGGTTGAAATCT+AGG - chr7.2:77517847-77517866 None:intergenic 35.0%
AAGTAAGAACCTTTCCAAGG+AGG - chr7.2:77516103-77516122 None:intergenic 40.0%
ACTATGGTCAAGAGATGTTG+AGG + chr7.2:77517026-77517045 MS.gene43133:CDS 40.0%
AGTGAGAGAGAAGGTTGAAT+TGG + chr7.2:77516304-77516323 MS.gene43133:CDS 40.0%
ATATAGTCTGATTCTGCTGC+AGG - chr7.2:77517867-77517886 None:intergenic 40.0%
ATGAGTATGTCAGGTGTCTA+TGG + chr7.2:77515969-77515988 MS.gene43133:CDS 40.0%
ATTCACCATGCCATACAATC+TGG + chr7.2:77516026-77516045 MS.gene43133:CDS 40.0%
CGAACACACCTTAAACTTGA+AGG + chr7.2:77517670-77517689 MS.gene43133:intron 40.0%
CTTGATACCTCTGACATCTA+TGG + chr7.2:77516059-77516078 MS.gene43133:CDS 40.0%
GAAGTTAGGATCACAAGGAA+TGG + chr7.2:77515920-77515939 MS.gene43133:CDS 40.0%
GCAGTAGGAAGAATGAAGTT+AGG + chr7.2:77515906-77515925 MS.gene43133:CDS 40.0%
GTATGTGTGAAATTGTGGAC+AGG + chr7.2:77516268-77516287 MS.gene43133:intron 40.0%
TCCAATAACAGCTGTGCAAT+TGG + chr7.2:77516996-77517015 MS.gene43133:CDS 40.0%
TCCAATTGCACAGCTGTTAT+TGG - chr7.2:77517000-77517019 None:intergenic 40.0%
TCGTCTTCCGATTGAAATCA+CGG + chr7.2:77516469-77516488 MS.gene43133:CDS 40.0%
TCTTCTGATTGTTGAAGCTG+AGG - chr7.2:77516962-77516981 None:intergenic 40.0%
TCTTTAGCAGATGCTGTCAA+GGG + chr7.2:77518072-77518091 MS.gene43133:CDS 40.0%
TGAATGAGAGCAATCATGTC+AGG - chr7.2:77516011-77516030 None:intergenic 40.0%
!! AATTGTGGACAGGCTTTAAG+AGG + chr7.2:77516278-77516297 MS.gene43133:intron 40.0%
!! ATTTTGGTGGTTCCAGGTAT+CGG - chr7.2:77517982-77518001 None:intergenic 40.0%
!!! TAGAACATTTTGGGATGCCA+AGG - chr7.2:77517718-77517737 None:intergenic 40.0%
AAGTCACTTAGCTCGACGTT+AGG - chr7.2:77516227-77516246 None:intergenic 45.0%
ACAGCTTTCCAAGAAGAGAG+TGG - chr7.2:77518147-77518166 None:intergenic 45.0%
AGAGCAATCATGTCAGGTTC+AGG - chr7.2:77516005-77516024 None:intergenic 45.0%
ATACTCCACCACTCTCTTCT+TGG + chr7.2:77518136-77518155 MS.gene43133:CDS 45.0%
ATCATGTCAGGTTCAGGCTT+AGG - chr7.2:77515999-77516018 None:intergenic 45.0%
CTGTCAAGGGTGGTAGATAT+GGG + chr7.2:77518085-77518104 MS.gene43133:CDS 45.0%
CTTGGTTGCATGAGTATGTC+AGG + chr7.2:77515960-77515979 MS.gene43133:CDS 45.0%
GAAAAGAATCCTCGTCCAAG+AGG - chr7.2:77517367-77517386 None:intergenic 45.0%
GATACCTGGAACCACCAAAA+TGG + chr7.2:77517981-77518000 MS.gene43133:CDS 45.0%
GTCTTTAGCAGATGCTGTCA+AGG + chr7.2:77518071-77518090 MS.gene43133:CDS 45.0%
TAACAGCTGTGCAATTGGAG+TGG + chr7.2:77517001-77517020 MS.gene43133:CDS 45.0%
TCTCAATCTACGATCAGCGA+TGG + chr7.2:77516717-77516736 MS.gene43133:intron 45.0%
TGACACCAGATTGTATGGCA+TGG - chr7.2:77516034-77516053 None:intergenic 45.0%
TGCAATTGGAGTGGTCACTA+TGG + chr7.2:77517010-77517029 MS.gene43133:CDS 45.0%
TGTCAAGGGTGGTAGATATG+GGG + chr7.2:77518086-77518105 MS.gene43133:CDS 45.0%
! AGCATGCTTTTCGACAAACC+TGG - chr7.2:77517435-77517454 None:intergenic 45.0%
! ATGCTTTTCGACAAACCTGG+CGG - chr7.2:77517432-77517451 None:intergenic 45.0%
! GGACGAGGATTCTTTTCATC+AGG + chr7.2:77517370-77517389 MS.gene43133:CDS 45.0%
! TGATTCTGCTGCAGGTTTTC+AGG - chr7.2:77517859-77517878 None:intergenic 45.0%
!! CTTTAATCGTTGGTGCTGCA+TGG + chr7.2:77517267-77517286 MS.gene43133:intron 45.0%
!! GTTTGGTGTCAAACACGTGT+TGG - chr7.2:77517241-77517260 None:intergenic 45.0%
!! TTCTCCATTTTGGTGGTTCC+AGG - chr7.2:77517988-77518007 None:intergenic 45.0%
!! TTTTGGTGGTTCCAGGTATC+GGG - chr7.2:77517981-77518000 None:intergenic 45.0%
AACAAAGGATGACTACCGCC+AGG + chr7.2:77517414-77517433 MS.gene43133:CDS 50.0%
AAGAGGAGGAGTGAGAGAGA+AGG + chr7.2:77516295-77516314 MS.gene43133:CDS 50.0%
ATGTCAGGTTCAGGCTTAGG+AGG - chr7.2:77515996-77516015 None:intergenic 50.0%
CAACGAACTCCTCCTTGGAA+AGG + chr7.2:77516091-77516110 MS.gene43133:CDS 50.0%
CAAGCTTCTCTCACGTAAGC+TGG - chr7.2:77516383-77516402 None:intergenic 50.0%
CATGCAACCAAGTCCTTGCA+AGG - chr7.2:77515952-77515971 None:intergenic 50.0%
CATGCCAATGCAAGCTGAGA+CGG - chr7.2:77517931-77517950 None:intergenic 50.0%
CTTTCCAAGGAGGAGTTCGT+TGG - chr7.2:77516093-77516112 None:intergenic 50.0%
GCATATAGTCCTCTTGGACG+AGG + chr7.2:77517355-77517374 MS.gene43133:CDS 50.0%
GCTGTCAAGGGTGGTAGATA+TGG + chr7.2:77518084-77518103 MS.gene43133:CDS 50.0%
GCTTTCCAAGAAGAGAGTGG+TGG - chr7.2:77518144-77518163 None:intergenic 50.0%
GTCGGTGACACTATCATGTC+AGG - chr7.2:77517542-77517561 None:intergenic 50.0%
TACTCCGTCTCAGCTTGCAT+TGG + chr7.2:77517924-77517943 MS.gene43133:CDS 50.0%
TTAGCAGATGCTGTCAAGGG+TGG + chr7.2:77518075-77518094 MS.gene43133:CDS 50.0%
TTGGCATGGCTTCATCACCA+AGG + chr7.2:77517943-77517962 MS.gene43133:CDS 50.0%
! GAAGTGTCCTTGCAAGGACT+TGG + chr7.2:77515942-77515961 MS.gene43133:CDS 50.0%
!! GGAATGGAAGTGTCCTTGCA+AGG + chr7.2:77515936-77515955 MS.gene43133:CDS 50.0%
!! TGTGGACAGGCTTTAAGAGG+AGG + chr7.2:77516281-77516300 MS.gene43133:intron 50.0%
AGGAGGAGTTCGTTGGTGTG+AGG - chr7.2:77516086-77516105 None:intergenic 55.0%
CACACCAACGAACTCCTCCT+TGG + chr7.2:77516086-77516105 MS.gene43133:CDS 55.0%
GGAGGAGTTCGTTGGTGTGA+GGG - chr7.2:77516085-77516104 None:intergenic 55.0%
GTGAGGGCCATAGATGTCAG+AGG - chr7.2:77516069-77516088 None:intergenic 55.0%
! CGTCTCAGCTTGCATTGGCA+TGG + chr7.2:77517929-77517948 MS.gene43133:CDS 55.0%
AACGACGTGTGCCCGATACC+TGG + chr7.2:77517967-77517986 MS.gene43133:CDS 60.0%
TCGGGCACACGTCGTTTCCT+TGG - chr7.2:77517963-77517982 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 77515900 77518170 77515900 ID=MS.gene43133
chr7.2 mRNA 77515900 77518170 77515900 ID=MS.gene43133.t1;Parent=MS.gene43133
chr7.2 exon 77515900 77516112 77515900 ID=MS.gene43133.t1.exon1;Parent=MS.gene43133.t1
chr7.2 CDS 77515900 77516112 77515900 ID=cds.MS.gene43133.t1;Parent=MS.gene43133.t1
chr7.2 exon 77516290 77516490 77516290 ID=MS.gene43133.t1.exon2;Parent=MS.gene43133.t1
chr7.2 CDS 77516290 77516490 77516290 ID=cds.MS.gene43133.t1;Parent=MS.gene43133.t1
chr7.2 exon 77516898 77517070 77516898 ID=MS.gene43133.t1.exon3;Parent=MS.gene43133.t1
chr7.2 CDS 77516898 77517070 77516898 ID=cds.MS.gene43133.t1;Parent=MS.gene43133.t1
chr7.2 exon 77517333 77517435 77517333 ID=MS.gene43133.t1.exon4;Parent=MS.gene43133.t1
chr7.2 CDS 77517333 77517435 77517333 ID=cds.MS.gene43133.t1;Parent=MS.gene43133.t1
chr7.2 exon 77517838 77518170 77517838 ID=MS.gene43133.t1.exon5;Parent=MS.gene43133.t1
chr7.2 CDS 77517838 77518170 77517838 ID=cds.MS.gene43133.t1;Parent=MS.gene43133.t1
Gene Sequence

>MS.gene43133

ATGACAGCAGTAGGAAGAATGAAGTTAGGATCACAAGGAATGGAAGTGTCCTTGCAAGGACTTGGTTGCATGAGTATGTCAGGTGTCTATGGTCCTCCTAAGCCTGAACCTGACATGATTGCTCTCATTCACCATGCCATACAATCTGGTGTCACTTTTCTTGATACCTCTGACATCTATGGCCCTCACACCAACGAACTCCTCCTTGGAAAGGTTCTTACTTTCTCTTCGCTTTTATTTAATTTTGCGATAAATTACAATTGATTTTGAGACATGATATTGATGATGAGTATTGAAATACAAATATACTACTCAAAGTTATTGCCTAACGTCGAGCTAAGTGACTTCGTAGAAATGTGATGAAATGTGTATGTGTGAAATTGTGGACAGGCTTTAAGAGGAGGAGTGAGAGAGAAGGTTGAATTGGCTACAAAGTTTGGAGCCAAATATATTGAAGGGAAATTTGAGATCTTTGGTGATCCAGCTTACGTGAGAGAAGCTTGTGAAGCTAGCTTGAAGAGACTTGATATTGATTGCATTGATCTCTATTATCAACATCGTATTGATACTCGTCTTCCGATTGAAATCACGGTTTGATTCTCAATAAAATTCTTTGATTCGATTTTGGTAGCTTAAATTGAGTACCATAAATAAGCATATGTTTGCTTCAGTAAATGAGATTAGTCCATATTGAAACACATTTTTATATTTATTACCTAAAATTAATGTTTGGATAAATTATTTTCAAAAAGGATATGATCGTCTTTACATTGCAGCTGACTATAGACCGTTAGATTAAGAGAGAAAGTTTTTTCTTTCTCAATCTACGATCAGCGATGGTAAAGTCGATTATAGATTTCATTCAAGATATGAGTTTATGTTTTTAAATATGTGAAATATGAGACTATATCAATCTAAGATGTGAAGTTAAGCTATTTGTTTACATGTATAATTGGACAATGTGTGATAACTTTAACTTTTCCCTTTTACTATGTAAGATTGGGGAGCTTAAGAAACTTGTTGAAGAAGGAAAAATAAAATACATTGGTTTGTCTGAAGCCTCAGCTTCAACAATCAGAAGAGCACATGCAGTTCATCCAATAACAGCTGTGCAATTGGAGTGGTCACTATGGTCAAGAGATGTTGAGGAAGAAATAATTCCAACTTGCAGGTTTGATTTAGTTTACAAACATTATGTTATGTTATCTAGCATTTCTCTCTGTGGTACTTTATTAAAATTGCAATGATATGAAGGTATAAAACACGACAACGATTGTAATTTAAGAAAATGGAAGTGATTGAATGTACTTACAAGTATTGGTGACATATGTCAATGCACCAACACGTGTTTGACACCAAACACACGTCTTTAATCGTTGGTGCTGCATGGATATGAAGTCTAACCTCATGAGTTATGATTTTTTTTTTCTTAGGGAACTTGGTATTGGAATTGTTGCATATAGTCCTCTTGGACGAGGATTCTTTTCATCAGGAACAAAGATTGTTGAGAACTTAACAAAGGATGACTACCGCCAGGTTTGTCGAAAAGCATGCTTTTACAGAAAATCATATCAATTACATGTTCATTATGTTCATCCACTCAACTGACAAGTATGAAATACTGGCACAAACATGGATACCTGACATGATAGTGTCACCGACACATAGACATCATTAATATCAGCAATAGTTTAAGAAAAACATAACTAACTAAATGCAATCACATGTTCAGTATTGTGTTGGTGTCAGACATTGAGACATGTCTCACATCGAACACACCTTAAACTTGAAGGTTTCGTGTCATATAGATTCTAACCTTGGCATCCCAAAATGTTCTATTTTAATTAACCCTACATCAATTATTAAATATCTTTATTATCTCATTGAATGTTCATCACTGATGCCTAAATATAATCTTCTATTTGTGTATGTGTGCAGTATATGCCTAGATTTCAACCTGAAAACCTGCAGCAGAATCAGACTATATTTGAGAGGGTTAATGAACTGGCTACAAAGAAGGGATGTACTCCGTCTCAGCTTGCATTGGCATGGCTTCATCACCAAGGAAACGACGTGTGCCCGATACCTGGAACCACCAAAATGGAGAACTTTAATCAAAACATTGGTGCTTTATCTGTTAAACTAACACAAGAAGAAATGGCAGAACTCGAGTCTTTAGCAGATGCTGTCAAGGGTGGTAGATATGGGGATGAAATAAGTACATGGAAGAATTCTGATACTCCACCACTCTCTTCTTGGAAAGCTGTGTAA
Protein sequence

>MS.gene43133.t1

MTAVGRMKLGSQGMEVSLQGLGCMSMSGVYGPPKPEPDMIALIHHAIQSGVTFLDTSDIYGPHTNELLLGKALRGGVREKVELATKFGAKYIEGKFEIFGDPAYVREACEASLKRLDIDCIDLYYQHRIDTRLPIEITIGELKKLVEEGKIKYIGLSEASASTIRRAHAVHPITAVQLEWSLWSRDVEEEIIPTCRELGIGIVAYSPLGRGFFSSGTKIVENLTKDDYRQYMPRFQPENLQQNQTIFERVNELATKKGCTPSQLALAWLHHQGNDVCPIPGTTKMENFNQNIGALSVKLTQEEMAELESLADAVKGGRYGDEISTWKNSDTPPLSSWKAV