AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43136

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43136.t1 MTR_7g021680 97.345 339 9 0 1 339 1 339 0.0 690
MS.gene43136.t1 MTR_7g021820 97.345 339 9 0 1 339 1 339 0.0 690
MS.gene43136.t1 MTR_7g021850 88.166 338 38 2 1 337 1 337 0.0 627
MS.gene43136.t1 MTR_7g114990 58.434 332 129 6 7 332 16 344 1.32e-139 400
MS.gene43136.t1 MTR_7g114980 59.050 337 127 6 6 332 13 348 3.01e-139 399
MS.gene43136.t1 MTR_1g102750 57.143 336 136 6 4 332 5 339 2.63e-133 383
MS.gene43136.t1 MTR_7g114970 56.250 336 139 6 6 334 9 343 4.53e-130 375
MS.gene43136.t1 MTR_7g115010 53.892 334 130 6 7 332 12 329 3.84e-125 362
MS.gene43136.t1 MTR_7g114980 61.566 281 101 4 6 280 13 292 1.97e-121 351
MS.gene43136.t1 MTR_1g102750 59.574 282 110 4 4 282 5 285 2.32e-118 344
MS.gene43136.t1 MTR_7g114970 60.432 278 106 4 6 280 9 285 2.44e-118 343
MS.gene43136.t1 MTR_7g114970 60.289 277 106 4 6 279 9 284 3.33e-117 340
MS.gene43136.t1 MTR_7g021825 93.151 146 10 0 157 302 15 160 5.09e-99 289
MS.gene43136.t1 MTR_7g114970 57.490 247 99 4 93 334 4 249 2.75e-95 283
MS.gene43136.t1 MTR_7g114970 63.918 194 68 2 6 197 9 202 2.38e-85 256
MS.gene43136.t1 MTR_7g021670 93.077 130 9 0 1 130 1 130 7.52e-85 257
MS.gene43136.t1 MTR_7g021670 89.815 108 11 0 231 338 145 252 2.89e-64 204
MS.gene43136.t1 MTR_7g114970 55.941 202 83 4 138 334 1 201 5.17e-72 222
MS.gene43136.t1 MTR_7g021685 91.304 69 6 0 1 69 1 69 2.63e-41 140
MS.gene43136.t1 MTR_3g449790 33.136 338 179 12 6 314 37 356 1.51e-34 130
MS.gene43136.t1 MTR_3g449790 32.544 338 175 12 6 314 37 350 6.22e-34 128
MS.gene43136.t1 MTR_1g102740 48.148 108 46 2 232 332 3 107 1.38e-28 107
MS.gene43136.t1 MTR_1g047250 27.607 326 202 11 9 312 6 319 9.46e-23 97.4
MS.gene43136.t1 MTR_3g092140 28.094 299 169 13 38 310 71 349 1.08e-22 99.0
MS.gene43136.t1 MTR_1g042730 28.367 349 197 13 8 309 65 407 1.24e-20 92.4
MS.gene43136.t1 MTR_4g092750 30.093 216 136 7 8 213 5 215 4.28e-16 77.8
MS.gene43136.t1 MTR_4g092750 30.093 216 136 7 8 213 5 215 5.11e-16 78.2
MS.gene43136.t1 MTR_4g092750 30.093 216 136 7 8 213 5 215 7.28e-16 77.4
MS.gene43136.t1 MTR_8g088160 26.199 271 150 10 14 238 49 315 2.90e-14 73.2
MS.gene43136.t1 MTR_4g036845 25.231 325 169 13 13 311 11 287 2.81e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43136.t1 AT1G60710 76.923 338 75 2 4 338 7 344 0.0 543
MS.gene43136.t1 AT1G60730 76.627 338 76 2 4 338 7 344 0.0 540
MS.gene43136.t1 AT1G60690 74.336 339 84 2 4 339 7 345 0.0 534
MS.gene43136.t1 AT1G60680 75.221 339 80 3 4 338 7 345 0.0 528
MS.gene43136.t1 AT1G60730 72.346 358 76 3 4 338 7 364 0.0 528
MS.gene43136.t1 AT1G10810 73.077 338 88 2 4 338 7 344 0.0 509
MS.gene43136.t1 AT1G60750 72.271 339 73 4 4 338 8 329 1.18e-178 498
MS.gene43136.t1 AT1G10810 72.694 271 71 2 71 338 22 292 2.54e-143 407
MS.gene43136.t1 AT1G60680 77.193 228 50 2 4 229 7 234 4.50e-127 364
MS.gene43136.t1 AT1G10810 74.569 232 58 1 4 234 7 238 8.73e-123 353
MS.gene43136.t1 AT1G60730 74.101 139 35 1 4 141 7 145 4.83e-71 219
MS.gene43136.t1 AT1G06690 30.422 332 196 12 6 314 48 367 5.76e-29 115
MS.gene43136.t1 AT5G53580 31.959 291 152 12 46 310 80 350 3.00e-28 113
MS.gene43136.t1 AT1G04690 28.247 308 189 10 9 296 6 301 2.93e-25 104
MS.gene43136.t1 AT4G33670 25.915 328 209 10 1 314 1 308 4.62e-21 92.4
MS.gene43136.t1 AT1G04420 26.197 355 207 14 8 314 60 407 5.77e-20 90.5

Find 80 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAACTGCACAGCTGTTAT+TGG 0.217840 7.2:-77547542 None:intergenic
CACACCAATGAAGTTCTTCT+TGG 0.223012 7.2:+77546847 MS.gene43136:CDS
CTTGATACTTCTGACATATA+TGG 0.245179 7.2:+77546820 MS.gene43136:CDS
ATACTCCACCACTCTCTTCT+TGG 0.280266 7.2:+77549061 MS.gene43136:CDS
GTTGAATTGGCTACTAAGTT+TGG 0.296790 7.2:+77547041 MS.gene43136:CDS
AAGAAGTAATTCCAACTTGC+AGG 0.316501 7.2:+77547594 MS.gene43136:CDS
ATTTGACAAAGTTAATGAAT+TGG 0.354570 7.2:+77548810 MS.gene43136:CDS
GTCTTTAGCAGATGCTGTTA+AGG 0.356223 7.2:+77548996 MS.gene43136:CDS
TTTATTAGGGAACTGGGTAT+TGG 0.362689 7.2:+77547859 MS.gene43136:intron
TGAGCTCTTCTGATTGTTGC+AGG 0.366246 7.2:-77547509 None:intergenic
ATCATGTCAGGTTCAGGTTT+AGG 0.367686 7.2:-77546757 None:intergenic
AAACAAACCGTGACTTCAAT+TGG 0.372449 7.2:-77547200 None:intergenic
GCTGTTAAGGGTGGTAGATA+TGG 0.379383 7.2:+77549009 MS.gene43136:CDS
AGAGCAATCATGTCAGGTTC+AGG 0.397066 7.2:-77546763 None:intergenic
CTTTCCAAGAAGAACTTCAT+TGG 0.408269 7.2:-77546851 None:intergenic
TGTGAGAGAGAAGGTTGAAT+TGG 0.411679 7.2:+77547028 MS.gene43136:CDS
CTGTTAAGGGTGGTAGATAT+GGG 0.417262 7.2:+77549010 MS.gene43136:CDS
AAAAGTGATGCCAGATTGTA+TGG 0.421570 7.2:-77546797 None:intergenic
CACCTCAGCTTGCACTAGCC+TGG 0.427311 7.2:+77548854 MS.gene43136:CDS
TAAACTAACACAAGAAGAAA+TGG 0.436815 7.2:+77548963 MS.gene43136:CDS
TCTTTAGCAGATGCTGTTAA+GGG 0.437336 7.2:+77548997 MS.gene43136:CDS
ATGAGCATGTCTGCTTTCTA+TGG 0.439792 7.2:+77546730 MS.gene43136:CDS
ATATAGTCTGATTCTGCTGC+AGG 0.450000 7.2:-77548789 None:intergenic
ATAGTTGCATATAGTCCTCT+TGG 0.466082 7.2:+77547883 MS.gene43136:CDS
ACAGTAGGAAGAATGAAGTT+AGG 0.469331 7.2:+77546667 MS.gene43136:CDS
TAATGAATTGGCTACAAAGA+AGG 0.471421 7.2:+77548822 MS.gene43136:CDS
GAAGTGTCCTTGCAAGGACT+TGG 0.472766 7.2:+77546703 MS.gene43136:CDS
ACTTGCCACAGGATGATTAC+CGG 0.478397 7.2:+77547944 MS.gene43136:CDS
TCGGGCACACGTCGTTTCCT+TGG 0.481328 7.2:-77548885 None:intergenic
ATTCACCATGCCATACAATC+TGG 0.484903 7.2:+77546787 MS.gene43136:CDS
TCCAATAACAGCTGTGCAGT+TGG 0.490955 7.2:+77547541 MS.gene43136:CDS
TTTGAAGGGTGTGAGAGAGA+AGG 0.495752 7.2:+77547019 MS.gene43136:CDS
GTTTCCTTGGTGATGAAGCC+AGG 0.503204 7.2:-77548872 None:intergenic
CAAGCTTCTCTCACATAGCT+TGG 0.512235 7.2:-77547104 None:intergenic
AACGACGTGTGCCCGATACC+TGG 0.512377 7.2:+77548892 MS.gene43136:CDS
TGCAGTTGGAGTGGTCACTC+TGG 0.512586 7.2:+77547555 MS.gene43136:CDS
CTTAAGAAACTTGTTGAAGA+GGG 0.519358 7.2:+77547452 MS.gene43136:CDS
TCTTCTGATTGTTGCAGGCG+AGG 0.520048 7.2:-77547504 None:intergenic
CAATGAAGTTCTTCTTGGAA+AGG 0.524852 7.2:+77546852 MS.gene43136:CDS
TTGGAGTTAGAGCTGGTGAT+GGG 0.526074 7.2:+77547060 MS.gene43136:CDS
AATGAATTGGCTACAAAGAA+GGG 0.535628 7.2:+77548823 MS.gene43136:CDS
ACTAAGTTTGGAGTTAGAGC+TGG 0.537266 7.2:+77547053 MS.gene43136:CDS
TAGTTGCATATAGTCCTCTT+GGG 0.540181 7.2:+77547884 MS.gene43136:CDS
CAGGCTAGTGCAAGCTGAGG+TGG 0.541943 7.2:-77548853 None:intergenic
TTTGGAGTTAGAGCTGGTGA+TGG 0.548871 7.2:+77547059 MS.gene43136:CDS
ATGGTTGCAAACCTGCAAGT+TGG 0.549594 7.2:-77547605 None:intergenic
GCATATAGTCCTCTTGGGCG+AGG 0.551187 7.2:+77547889 MS.gene43136:CDS
TCGTCTTCCAATTGAAGTCA+CGG 0.553776 7.2:+77547193 MS.gene43136:CDS
GAGGGGAAGATAAAATACAT+TGG 0.556959 7.2:+77547470 MS.gene43136:CDS
TGAATGAGAGCAATCATGTC+AGG 0.558885 7.2:-77546769 None:intergenic
GAAGTTAGGATCACAAGGCA+TGG 0.564034 7.2:+77546681 MS.gene43136:CDS
GAAAACTTGAATCAAAACAT+TGG 0.569167 7.2:+77548928 MS.gene43136:CDS
CTATGTGAGAGAAGCTTGTG+AGG 0.569710 7.2:+77547109 MS.gene43136:CDS
GAAGAACTTCATTGGTGTGA+GGG 0.571972 7.2:-77546843 None:intergenic
GTTAATTGACAACTTGCCAC+AGG 0.576617 7.2:+77547933 MS.gene43136:CDS
GCTTAAGAAACTTGTTGAAG+AGG 0.577571 7.2:+77547451 MS.gene43136:CDS
GTCACTCTGGTCAAGAGATG+TGG 0.577946 7.2:+77547568 MS.gene43136:CDS
ATGTCAGGTTCAGGTTTAGG+AGG 0.578905 7.2:-77546754 None:intergenic
CATGCATCCAAGTCCTTGCA+AGG 0.583471 7.2:-77546710 None:intergenic
GGCATGGAAGTGTCCTTGCA+AGG 0.583936 7.2:+77546697 MS.gene43136:CDS
AAAATACATTGGTTTGTCTG+AGG 0.590704 7.2:+77547481 MS.gene43136:CDS
TAACAGCTGTGCAGTTGGAG+TGG 0.606322 7.2:+77547546 MS.gene43136:CDS
GCCACAGGATGATTACCGGA+AGG 0.619947 7.2:+77547948 MS.gene43136:CDS
GTCATTGCAATGGCAACAGT+AGG 0.623964 7.2:+77546652 None:intergenic
TTAAGAAACTTGTTGAAGAG+GGG 0.630026 7.2:+77547453 MS.gene43136:CDS
AGAAGAACTTCATTGGTGTG+AGG 0.636443 7.2:-77546844 None:intergenic
GAAAAGAATCCTCGCCCAAG+AGG 0.639338 7.2:-77547898 None:intergenic
TTAGCAGATGCTGTTAAGGG+TGG 0.639881 7.2:+77549000 MS.gene43136:CDS
ACTCTGGTCAAGAGATGTGG+AGG 0.640036 7.2:+77547571 MS.gene43136:CDS
AGAATGAAGTTAGGATCACA+AGG 0.646380 7.2:+77546676 MS.gene43136:CDS
GCTTTCCAAGAAGAGAGTGG+TGG 0.651055 7.2:-77549066 None:intergenic
ATGGGGATGAGATAAGTACA+TGG 0.656484 7.2:+77549028 MS.gene43136:CDS
ACCTTCCGGTAATCATCCTG+TGG 0.662538 7.2:-77547949 None:intergenic
ACAGCTTTCCAAGAAGAGAG+TGG 0.666396 7.2:-77549069 None:intergenic
TATGTGAGAGAAGCTTGTGA+GGG 0.666442 7.2:+77547110 MS.gene43136:CDS
AGCCAGGCTAGTGCAAGCTG+AGG 0.684598 7.2:-77548856 None:intergenic
TGTTAAGGGTGGTAGATATG+GGG 0.698011 7.2:+77549011 MS.gene43136:CDS
TGATGCCAGATTGTATGGCA+TGG 0.700963 7.2:-77546792 None:intergenic
GATGGGAAATTTGAGATCTG+TGG 0.703407 7.2:+77547077 MS.gene43136:CDS
CTAGCCTGGCTTCATCACCA+AGG 0.706704 7.2:+77548868 MS.gene43136:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATTTTGATATTTTGATT+TGG + chr7.2:77546931-77546950 MS.gene43136:intron 10.0%
!!! ATTTATGATTTTCTTTATTA+GGG + chr7.2:77547846-77547865 MS.gene43136:intron 10.0%
!!! TTATTTTGATATTTTGATTT+GGG + chr7.2:77546932-77546951 MS.gene43136:intron 10.0%
!! AATCAAAAGAAAAACTAAAA+GGG - chr7.2:77546894-77546913 None:intergenic 15.0%
!! ACAATTTAAACAAGTAAATT+TGG - chr7.2:77548137-77548156 None:intergenic 15.0%
!! TAATCAAAAGAAAAACTAAA+AGG - chr7.2:77546895-77546914 None:intergenic 15.0%
!! TACTATATAACAAAACATAA+TGG - chr7.2:77547627-77547646 None:intergenic 15.0%
!!! GATTTATGATTTTCTTTATT+AGG + chr7.2:77547845-77547864 MS.gene43136:intron 15.0%
!! ATATATACACAAATCAATCA+TGG + chr7.2:77548500-77548519 MS.gene43136:intron 20.0%
!! ATTTGACAAAGTTAATGAAT+TGG + chr7.2:77548810-77548829 MS.gene43136:CDS 20.0%
!! TTATATAGTATTTCTCTTTC+TGG + chr7.2:77547637-77547656 MS.gene43136:intron 20.0%
!! TTGAATGTAATTACATATGT+TGG + chr7.2:77547754-77547773 MS.gene43136:intron 20.0%
!!! AACCAAAAGGTATATAAATT+AGG - chr7.2:77547329-77547348 None:intergenic 20.0%
!!! AATTTATATACCTTTTGGTT+TGG + chr7.2:77547329-77547348 MS.gene43136:intron 20.0%
!!! GATTGTGAATTTTTATGAAT+AGG + chr7.2:77546995-77547014 MS.gene43136:intron 20.0%
!!! TTCCTAATTTATATACCTTT+TGG + chr7.2:77547324-77547343 MS.gene43136:intron 20.0%
!!! TTCTTTTGATTAGAATTTCT+GGG + chr7.2:77546903-77546922 MS.gene43136:intron 20.0%
!!! TTGATTCAAGTTTTCAATTT+TGG - chr7.2:77548923-77548942 None:intergenic 20.0%
!!! TTGTGTATATATGTACTTTT+CGG - chr7.2:77548492-77548511 None:intergenic 20.0%
!!! TTTCTTTTGATTAGAATTTC+TGG + chr7.2:77546902-77546921 MS.gene43136:intron 20.0%
! AAATATTCAACCAAACCAAA+AGG - chr7.2:77547342-77547361 None:intergenic 25.0%
! AAGCTACCAAAATCAAATTA+GGG - chr7.2:77547242-77547261 None:intergenic 25.0%
! AAGTAATTCACATGAATTTC+TGG - chr7.2:77547996-77548015 None:intergenic 25.0%
! ATATATAAAATGACAATGCG+TGG + chr7.2:77547388-77547407 MS.gene43136:intron 25.0%
! GAAAACTTGAATCAAAACAT+TGG + chr7.2:77548928-77548947 MS.gene43136:CDS 25.0%
! TAAACTAACACAAGAAGAAA+TGG + chr7.2:77548963-77548982 MS.gene43136:CDS 25.0%
! TAAGCTACCAAAATCAAATT+AGG - chr7.2:77547243-77547262 None:intergenic 25.0%
! TTTAAATTGTGTAATGTGTG+TGG + chr7.2:77548147-77548166 MS.gene43136:intron 25.0%
! TTTAGTCCATAAAACAGTTT+GGG + chr7.2:77548225-77548244 MS.gene43136:intron 25.0%
!! ATTTTCTTTATTAGGGAACT+GGG + chr7.2:77547853-77547872 MS.gene43136:intron 25.0%
!! TTTTAGTCCATAAAACAGTT+TGG + chr7.2:77548224-77548243 MS.gene43136:intron 25.0%
!!! ATTCAAGTTTTCAATTTTGG+TGG - chr7.2:77548920-77548939 None:intergenic 25.0%
!!! TTTTATGAATAGGCTTTGAA+GGG + chr7.2:77547005-77547024 MS.gene43136:intron 25.0%
!!! TTTTTATGAATAGGCTTTGA+AGG + chr7.2:77547004-77547023 MS.gene43136:intron 25.0%
AAATTAGGGAGAACTGAAAA+GGG - chr7.2:77547228-77547247 None:intergenic 30.0%
AATGAATTGGCTACAAAGAA+GGG + chr7.2:77548823-77548842 MS.gene43136:CDS 30.0%
ATACAAACAGGTCCATATTA+TGG + chr7.2:77548314-77548333 MS.gene43136:intron 30.0%
CAATCTTCTCAAATTGTTAC+CGG - chr7.2:77547728-77547747 None:intergenic 30.0%
CAGAACTCTATAATACAAAC+AGG + chr7.2:77548302-77548321 MS.gene43136:intron 30.0%
CATCAAGTAGTTCATTTCAT+AGG - chr7.2:77547366-77547385 None:intergenic 30.0%
CTACAGAATATGGTATGTTA+TGG - chr7.2:77548370-77548389 None:intergenic 30.0%
CTTGATACTTCTGACATATA+TGG + chr7.2:77546820-77546839 MS.gene43136:CDS 30.0%
TAAAATGCAGCTACAGAATA+TGG - chr7.2:77548380-77548399 None:intergenic 30.0%
TAATGAATTGGCTACAAAGA+AGG + chr7.2:77548822-77548841 MS.gene43136:CDS 30.0%
! AAATTTGGTAATTCTGACCA+TGG - chr7.2:77548122-77548141 None:intergenic 30.0%
! ATATTCTGTAGCTGCATTTT+AGG + chr7.2:77548379-77548398 MS.gene43136:intron 30.0%
! GATTTTCTTTATTAGGGAAC+TGG + chr7.2:77547852-77547871 MS.gene43136:intron 30.0%
! TCATTTTCTTAGTATCAGTG+TGG - chr7.2:77548464-77548483 None:intergenic 30.0%
! TGATCACAATAATCGTTTTG+AGG + chr7.2:77548030-77548049 MS.gene43136:intron 30.0%
! TGGGTTTTCTTTGTAAGATT+GGG + chr7.2:77547426-77547445 MS.gene43136:intron 30.0%
! TTGGGTTTTCTTTGTAAGAT+TGG + chr7.2:77547425-77547444 MS.gene43136:intron 30.0%
!! AAAATACATTGGTTTGTCTG+AGG + chr7.2:77547481-77547500 MS.gene43136:CDS 30.0%
!! AGTTCTCCCTAATTTGATTT+TGG + chr7.2:77547233-77547252 MS.gene43136:intron 30.0%
!! CTTAAGAAACTTGTTGAAGA+GGG + chr7.2:77547452-77547471 MS.gene43136:CDS 30.0%
!! TTAAGAAACTTGTTGAAGAG+GGG + chr7.2:77547453-77547472 MS.gene43136:CDS 30.0%
AAAAGTGATGCCAGATTGTA+TGG - chr7.2:77546800-77546819 None:intergenic 35.0%
AAACAAACCGTGACTTCAAT+TGG - chr7.2:77547203-77547222 None:intergenic 35.0%
AAGAAGTAATTCCAACTTGC+AGG + chr7.2:77547594-77547613 MS.gene43136:CDS 35.0%
AATTCTTTCCACAAACCTTC+CGG - chr7.2:77547966-77547985 None:intergenic 35.0%
ACAGTAGGAAGAATGAAGTT+AGG + chr7.2:77546667-77546686 MS.gene43136:CDS 35.0%
ACTTACATCGTTGATCAATC+AGG + chr7.2:77548551-77548570 MS.gene43136:intron 35.0%
AGAATGAAGTTAGGATCACA+AGG + chr7.2:77546676-77546695 MS.gene43136:CDS 35.0%
ATAGTTGCATATAGTCCTCT+TGG + chr7.2:77547883-77547902 MS.gene43136:CDS 35.0%
CAAATTAGGGAGAACTGAAA+AGG - chr7.2:77547229-77547248 None:intergenic 35.0%
CAATGAAGTTCTTCTTGGAA+AGG + chr7.2:77546852-77546871 MS.gene43136:CDS 35.0%
CCATAAAACAGTTTGGGAAT+AGG + chr7.2:77548231-77548250 MS.gene43136:intron 35.0%
CTTACATCGTTGATCAATCA+GGG + chr7.2:77548552-77548571 MS.gene43136:intron 35.0%
CTTTCCAAGAAGAACTTCAT+TGG - chr7.2:77546854-77546873 None:intergenic 35.0%
GAGGGGAAGATAAAATACAT+TGG + chr7.2:77547470-77547489 MS.gene43136:CDS 35.0%
GATCAACGATGTAAGTTAGA+GGG - chr7.2:77548547-77548566 None:intergenic 35.0%
GTTGAATTGGCTACTAAGTT+TGG + chr7.2:77547041-77547060 MS.gene43136:CDS 35.0%
TAGTTGCATATAGTCCTCTT+GGG + chr7.2:77547884-77547903 MS.gene43136:CDS 35.0%
TCACACATAGTGCCATAATA+TGG - chr7.2:77548329-77548348 None:intergenic 35.0%
TCTTTAGCAGATGCTGTTAA+GGG + chr7.2:77548997-77549016 MS.gene43136:CDS 35.0%
TGATCAACGATGTAAGTTAG+AGG - chr7.2:77548548-77548567 None:intergenic 35.0%
TTCATAGGATACAAGATGTC+AGG - chr7.2:77548419-77548438 None:intergenic 35.0%
TTGTGATAGACCAAACAAAG+AGG - chr7.2:77548595-77548614 None:intergenic 35.0%
TTTATTAGGGAACTGGGTAT+TGG + chr7.2:77547859-77547878 MS.gene43136:intron 35.0%
! ACTAAAAGGGTATGAGAAAG+TGG - chr7.2:77546881-77546900 None:intergenic 35.0%
! CCTATTCCCAAACTGTTTTA+TGG - chr7.2:77548234-77548253 None:intergenic 35.0%
! GGGTTTTCTTTGTAAGATTG+GGG + chr7.2:77547427-77547446 MS.gene43136:intron 35.0%
!! GCTTAAGAAACTTGTTGAAG+AGG + chr7.2:77547451-77547470 MS.gene43136:CDS 35.0%
!! GTTTTGTTGCATGTGATATG+AGG + chr7.2:77546954-77546973 MS.gene43136:intron 35.0%
!! TTTTCAATTTTGGTGGTTCC+AGG - chr7.2:77548913-77548932 None:intergenic 35.0%
!!! GTGGTGACTTCAACTTTTTT+GGG + chr7.2:77547407-77547426 MS.gene43136:intron 35.0%
AGAAGAACTTCATTGGTGTG+AGG - chr7.2:77546847-77546866 None:intergenic 40.0%
AGCACAGACAATAACACGTT+TGG - chr7.2:77547800-77547819 None:intergenic 40.0%
ATATAGTCTGATTCTGCTGC+AGG - chr7.2:77548792-77548811 None:intergenic 40.0%
ATGAGCATGTCTGCTTTCTA+TGG + chr7.2:77546730-77546749 MS.gene43136:CDS 40.0%
ATGGGGATGAGATAAGTACA+TGG + chr7.2:77549028-77549047 MS.gene43136:CDS 40.0%
ATTCACCATGCCATACAATC+TGG + chr7.2:77546787-77546806 MS.gene43136:CDS 40.0%
CACACCAATGAAGTTCTTCT+TGG + chr7.2:77546847-77546866 MS.gene43136:CDS 40.0%
CTCATAACAGCCTCTTTGTT+TGG + chr7.2:77548582-77548601 MS.gene43136:intron 40.0%
CTGTTAAGGGTGGTAGATAT+GGG + chr7.2:77549010-77549029 MS.gene43136:CDS 40.0%
GAAGAACTTCATTGGTGTGA+GGG - chr7.2:77546846-77546865 None:intergenic 40.0%
GTCTTTAGCAGATGCTGTTA+AGG + chr7.2:77548996-77549015 MS.gene43136:CDS 40.0%
GTTAATTGACAACTTGCCAC+AGG + chr7.2:77547933-77547952 MS.gene43136:CDS 40.0%
TATGTGAGAGAAGCTTGTGA+GGG + chr7.2:77547110-77547129 MS.gene43136:CDS 40.0%
TCGTCTTCCAATTGAAGTCA+CGG + chr7.2:77547193-77547212 MS.gene43136:CDS 40.0%
TGAATGAGAGCAATCATGTC+AGG - chr7.2:77546772-77546791 None:intergenic 40.0%
TGTGAGAGAGAAGGTTGAAT+TGG + chr7.2:77547028-77547047 MS.gene43136:CDS 40.0%
TGTTAAGGGTGGTAGATATG+GGG + chr7.2:77549011-77549030 MS.gene43136:CDS 40.0%
! ACTAAGTTTGGAGTTAGAGC+TGG + chr7.2:77547053-77547072 MS.gene43136:CDS 40.0%
! ATCATGTCAGGTTCAGGTTT+AGG - chr7.2:77546760-77546779 None:intergenic 40.0%
! GATGGGAAATTTGAGATCTG+TGG + chr7.2:77547077-77547096 MS.gene43136:CDS 40.0%
! TGAAGCACTTACATAGACAC+TGG + chr7.2:77547692-77547711 MS.gene43136:intron 40.0%
!! ATTTTGGTGGTTCCAGGTAT+CGG - chr7.2:77548907-77548926 None:intergenic 40.0%
!!! AGGTTTTCAGGTTGAAATCG+AGG - chr7.2:77548772-77548791 None:intergenic 40.0%
!!! CGTGGTGACTTCAACTTTTT+TGG + chr7.2:77547406-77547425 MS.gene43136:intron 40.0%
ACAGCTTTCCAAGAAGAGAG+TGG - chr7.2:77549072-77549091 None:intergenic 45.0%
ACTTGCCACAGGATGATTAC+CGG + chr7.2:77547944-77547963 MS.gene43136:CDS 45.0%
AGAGCAATCATGTCAGGTTC+AGG - chr7.2:77546766-77546785 None:intergenic 45.0%
ATACTCCACCACTCTCTTCT+TGG + chr7.2:77549061-77549080 MS.gene43136:CDS 45.0%
ATGGTTGCAAACCTGCAAGT+TGG - chr7.2:77547608-77547627 None:intergenic 45.0%
CAAGCTTCTCTCACATAGCT+TGG - chr7.2:77547107-77547126 None:intergenic 45.0%
CTATGTGAGAGAAGCTTGTG+AGG + chr7.2:77547109-77547128 MS.gene43136:CDS 45.0%
GAAGTTAGGATCACAAGGCA+TGG + chr7.2:77546681-77546700 MS.gene43136:CDS 45.0%
GATGATTACCGGAAGGTTTG+TGG + chr7.2:77547955-77547974 MS.gene43136:intron 45.0%
GCTGTTAAGGGTGGTAGATA+TGG + chr7.2:77549009-77549028 MS.gene43136:CDS 45.0%
TCCAACTGCACAGCTGTTAT+TGG - chr7.2:77547545-77547564 None:intergenic 45.0%
TCCAATAACAGCTGTGCAGT+TGG + chr7.2:77547541-77547560 MS.gene43136:CDS 45.0%
TGAGCTCTTCTGATTGTTGC+AGG - chr7.2:77547512-77547531 None:intergenic 45.0%
TGATGCCAGATTGTATGGCA+TGG - chr7.2:77546795-77546814 None:intergenic 45.0%
TTAGCAGATGCTGTTAAGGG+TGG + chr7.2:77549000-77549019 MS.gene43136:CDS 45.0%
TTGGAGTTAGAGCTGGTGAT+GGG + chr7.2:77547060-77547079 MS.gene43136:CDS 45.0%
! ATGTCAGGTTCAGGTTTAGG+AGG - chr7.2:77546757-77546776 None:intergenic 45.0%
! TGATTCTGCTGCAGGTTTTC+AGG - chr7.2:77548784-77548803 None:intergenic 45.0%
! TGTCTGTGTTGGTGCTTCAT+AGG - chr7.2:77548434-77548453 None:intergenic 45.0%
! TTTGAAGGGTGTGAGAGAGA+AGG + chr7.2:77547019-77547038 MS.gene43136:CDS 45.0%
! TTTGGAGTTAGAGCTGGTGA+TGG + chr7.2:77547059-77547078 MS.gene43136:CDS 45.0%
!! TGTTGGTGTCGTCTTAGTGT+CGG + chr7.2:77547771-77547790 MS.gene43136:intron 45.0%
!! TTTTGGTGGTTCCAGGTATC+GGG - chr7.2:77548906-77548925 None:intergenic 45.0%
!! ATATATATAAGATTATATTA+AGG - chr7.2:77548721-77548740 None:intergenic 5.0%
ACCTTCCGGTAATCATCCTG+TGG - chr7.2:77547952-77547971 None:intergenic 50.0%
ACTCTGGTCAAGAGATGTGG+AGG + chr7.2:77547571-77547590 MS.gene43136:CDS 50.0%
CATGCATCCAAGTCCTTGCA+AGG - chr7.2:77546713-77546732 None:intergenic 50.0%
GAAAAGAATCCTCGCCCAAG+AGG - chr7.2:77547901-77547920 None:intergenic 50.0%
GCTTTCCAAGAAGAGAGTGG+TGG - chr7.2:77549069-77549088 None:intergenic 50.0%
GTCACTCTGGTCAAGAGATG+TGG + chr7.2:77547568-77547587 MS.gene43136:CDS 50.0%
GTGGTATCCAGTGTCTGTGT+TGG - chr7.2:77548445-77548464 None:intergenic 50.0%
TAACAGCTGTGCAGTTGGAG+TGG + chr7.2:77547546-77547565 MS.gene43136:CDS 50.0%
TCTTCTGATTGTTGCAGGCG+AGG - chr7.2:77547507-77547526 None:intergenic 50.0%
! GAAGTGTCCTTGCAAGGACT+TGG + chr7.2:77546703-77546722 MS.gene43136:CDS 50.0%
! GGGCGAGGATTCTTTTCAAC+AGG + chr7.2:77547904-77547923 MS.gene43136:CDS 50.0%
! TGAAGCACCAACACAGACAC+TGG + chr7.2:77548435-77548454 MS.gene43136:intron 50.0%
!! GTTCACAGACTGATTCGCCA+TGG + chr7.2:77548102-77548121 MS.gene43136:intron 50.0%
!! GTTTCCTTGGTGATGAAGCC+AGG - chr7.2:77548875-77548894 None:intergenic 50.0%
AGACACTGGACACGACACTC+CGG + chr7.2:77547706-77547725 MS.gene43136:intron 55.0%
CTAGCCTGGCTTCATCACCA+AGG + chr7.2:77548868-77548887 MS.gene43136:CDS 55.0%
GCATATAGTCCTCTTGGGCG+AGG + chr7.2:77547889-77547908 MS.gene43136:CDS 55.0%
GCCACAGGATGATTACCGGA+AGG + chr7.2:77547948-77547967 MS.gene43136:CDS 55.0%
TGCAGTTGGAGTGGTCACTC+TGG + chr7.2:77547555-77547574 MS.gene43136:CDS 55.0%
!! GGCATGGAAGTGTCCTTGCA+AGG + chr7.2:77546697-77546716 MS.gene43136:CDS 55.0%
AACGACGTGTGCCCGATACC+TGG + chr7.2:77548892-77548911 MS.gene43136:CDS 60.0%
AGCCAGGCTAGTGCAAGCTG+AGG - chr7.2:77548859-77548878 None:intergenic 60.0%
CACCTCAGCTTGCACTAGCC+TGG + chr7.2:77548854-77548873 MS.gene43136:CDS 60.0%
CAGGCTAGTGCAAGCTGAGG+TGG - chr7.2:77548856-77548875 None:intergenic 60.0%
TCGGGCACACGTCGTTTCCT+TGG - chr7.2:77548888-77548907 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 77546661 77549095 77546661 ID=MS.gene43136
chr7.2 mRNA 77546661 77549095 77546661 ID=MS.gene43136.t1;Parent=MS.gene43136
chr7.2 exon 77546661 77546873 77546661 ID=MS.gene43136.t1.exon1;Parent=MS.gene43136.t1
chr7.2 CDS 77546661 77546873 77546661 ID=cds.MS.gene43136.t1;Parent=MS.gene43136.t1
chr7.2 exon 77547017 77547214 77547017 ID=MS.gene43136.t1.exon2;Parent=MS.gene43136.t1
chr7.2 CDS 77547017 77547214 77547017 ID=cds.MS.gene43136.t1;Parent=MS.gene43136.t1
chr7.2 exon 77547443 77547615 77547443 ID=MS.gene43136.t1.exon3;Parent=MS.gene43136.t1
chr7.2 CDS 77547443 77547615 77547443 ID=cds.MS.gene43136.t1;Parent=MS.gene43136.t1
chr7.2 exon 77547867 77547969 77547867 ID=MS.gene43136.t1.exon4;Parent=MS.gene43136.t1
chr7.2 CDS 77547867 77547969 77547867 ID=cds.MS.gene43136.t1;Parent=MS.gene43136.t1
chr7.2 exon 77548763 77549095 77548763 ID=MS.gene43136.t1.exon5;Parent=MS.gene43136.t1
chr7.2 CDS 77548763 77549095 77548763 ID=cds.MS.gene43136.t1;Parent=MS.gene43136.t1
Gene Sequence

>MS.gene43136

ATGGCAACAGTAGGAAGAATGAAGTTAGGATCACAAGGCATGGAAGTGTCCTTGCAAGGACTTGGATGCATGAGCATGTCTGCTTTCTATGGTCCTCCTAAACCTGAACCTGACATGATTGCTCTCATTCACCATGCCATACAATCTGGCATCACTTTTCTTGATACTTCTGACATATATGGCCCTCACACCAATGAAGTTCTTCTTGGAAAGGTTACCACTTTCTCATACCCTTTTAGTTTTTCTTTTGATTAGAATTTCTGGGTATGTATTATTTTGATATTTTGATTTGGGTTTTGTTGCATGTGATATGAGGATGAATGAAATTGTATATGATTGTGAATTTTTATGAATAGGCTTTGAAGGGTGTGAGAGAGAAGGTTGAATTGGCTACTAAGTTTGGAGTTAGAGCTGGTGATGGGAAATTTGAGATCTGTGGTGATCCAAGCTATGTGAGAGAAGCTTGTGAGGGTAGTTTGAAGAGACTTGATATTGATTGTATTGATCTCTATTATCAACATCGTATTGATACTCGTCTTCCAATTGAAGTCACGGTTTGTTTAACCCTTTTCAGTTCTCCCTAATTTGATTTTGGTAGCTTAATTCAGTTCTCCCTAATTTGATTTTGGTAGCTTAAACTTTTGAATCATGATATGTGATATTTTCCTAATTTATATACCTTTTGGTTTGGTTGAATATTTTCCTATGAAATGAACTACTTGATGACATATATAAAATGACAATGCGTGGTGACTTCAACTTTTTTGGGTTTTCTTTGTAAGATTGGGGAGCTTAAGAAACTTGTTGAAGAGGGGAAGATAAAATACATTGGTTTGTCTGAGGCCTCGCCTGCAACAATCAGAAGAGCTCACGCAGTTCATCCAATAACAGCTGTGCAGTTGGAGTGGTCACTCTGGTCAAGAGATGTGGAGGAAGAAGTAATTCCAACTTGCAGGTTTGCAACCATTATGTTTTGTTATATAGTATTTCTCTTTCTGGTACTTTGATAAAATTGCAATAATATGAAGCTATGAAGCACTTACATAGACACTGGACACGACACTCCGGTAACAATTTGAGAAGATTGAAGTAATTGAATGTAATTACATATGTTGGTGTCGTCTTAGTGTCGGAGACCAAACGTGTTATTGTCTGTGCTACAAAGATATGAAGTCTAACTTCATGATTTATGATTTTCTTTATTAGGGAACTGGGTATTGGAATAGTTGCATATAGTCCTCTTGGGCGAGGATTCTTTTCAACAGGAACAAAGTTAATTGACAACTTGCCACAGGATGATTACCGGAAGGTTTGTGGAAAGAATTTTCTTTTCCAGAAATTCATGTGAATTACTTGTTTGTTTTGTTAATGATCACAATAATCGTTTTGAGGAATTATGTATACTTATTTTCTGTAGCAATCTTCATTACACAATAATAGTGTTCACAGACTGATTCGCCATGGTCAGAATTACCAAATTTACTTGTTTAAATTGTGTAATGTGTGTGGTTGTTGTATATTGAGTTGACTATAATTCAAAGTAAAAATATAGTCGATGTACATTTTTAGTCCATAAAACAGTTTGGGAATAGGCGTAACAGTAATAGATGACACTTTAAGTTGATGATAGTGAACACAATTCAGAACTCTATAATACAAACAGGTCCATATTATGGCACTATGTGTGAATGTGTGCGCATGCGATTCCATAACATACCATATTCTGTAGCTGCATTTTAGGATTTTTTTTTACTACCTGACATCTTGTATCCTATGAAGCACCAACACAGACACTGGATACCACACTGATACTAAGAAAATGATGCAGCCGAAAAGTACATATATACACAAATCAATCATGGTCACACATTTAGATCATAAATCCCTCTAACTTACATCGTTGATCAATCAGGGCAATTATCTCATAACAGCCTCTTTGTTTGGTCTATCACAATATTAACTTTTAATACTGATATATTTCATAATCAATTATATTGAATGTAATCACATGAGTCAGAGAAATTGCAAATTTTGTCTTTTAATGTCTATCAGTGATACCTTAATATAATCTTATATATATCTACGTATGTAATTTGTTGCAGCATTTGCCTCGATTTCAACCTGAAAACCTGCAGCAGAATCAGACTATATTTGACAAAGTTAATGAATTGGCTACAAAGAAGGGATGTACTCCACCTCAGCTTGCACTAGCCTGGCTTCATCACCAAGGAAACGACGTGTGCCCGATACCTGGAACCACCAAAATTGAAAACTTGAATCAAAACATTGGTGCTTTATCTGTTAAACTAACACAAGAAGAAATGGCAGAACTCGAGTCTTTAGCAGATGCTGTTAAGGGTGGTAGATATGGGGATGAGATAAGTACATGGAAGAACTCTGATACTCCACCACTCTCTTCTTGGAAAGCTGTGTAA
Protein sequence

>MS.gene43136.t1

MATVGRMKLGSQGMEVSLQGLGCMSMSAFYGPPKPEPDMIALIHHAIQSGITFLDTSDIYGPHTNEVLLGKALKGVREKVELATKFGVRAGDGKFEICGDPSYVREACEGSLKRLDIDCIDLYYQHRIDTRLPIEVTIGELKKLVEEGKIKYIGLSEASPATIRRAHAVHPITAVQLEWSLWSRDVEEEVIPTCRELGIGIVAYSPLGRGFFSTGTKLIDNLPQDDYRKHLPRFQPENLQQNQTIFDKVNELATKKGCTPPQLALAWLHHQGNDVCPIPGTTKIENLNQNIGALSVKLTQEEMAELESLADAVKGGRYGDEISTWKNSDTPPLSSWKAV