Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44915.t1 | XP_003613352.1 | 95.1 | 223 | 11 | 0 | 1 | 223 | 1 | 223 | 1.80E-106 | 396.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44915.t1 | G7K465 | 95.1 | 223 | 11 | 0 | 1 | 223 | 1 | 223 | 1.3e-106 | 396.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049297 | MS.gene44915 | 0.803837 | 2.88E-49 | -1.69E-46 |
| MS.gene049351 | MS.gene44915 | 0.868452 | 6.46E-66 | -1.69E-46 |
| MS.gene049491 | MS.gene44915 | 0.829552 | 4.83E-55 | -1.69E-46 |
| MS.gene049681 | MS.gene44915 | 0.812135 | 4.94E-51 | -1.69E-46 |
| MS.gene049758 | MS.gene44915 | 0.807765 | 4.31E-50 | -1.69E-46 |
| MS.gene050076 | MS.gene44915 | 0.819393 | 1.19E-52 | -1.69E-46 |
| MS.gene050099 | MS.gene44915 | 0.899233 | 2.43E-77 | -1.69E-46 |
| MS.gene050231 | MS.gene44915 | 0.834996 | 2.17E-56 | -1.69E-46 |
| MS.gene050301 | MS.gene44915 | 0.896412 | 3.78E-76 | -1.69E-46 |
| MS.gene050558 | MS.gene44915 | 0.804486 | 2.11E-49 | -1.69E-46 |
| MS.gene050767 | MS.gene44915 | 0.80174 | 7.81E-49 | -1.69E-46 |
| MS.gene050770 | MS.gene44915 | 0.804687 | 1.92E-49 | -1.69E-46 |
| MS.gene050783 | MS.gene44915 | 0.811113 | 8.24E-51 | -1.69E-46 |
| MS.gene050812 | MS.gene44915 | 0.807425 | 5.09E-50 | -1.69E-46 |
| MS.gene05101 | MS.gene44915 | 0.880328 | 6.01E-70 | -1.69E-46 |
| MS.gene051255 | MS.gene44915 | 0.809277 | 2.05E-50 | -1.69E-46 |
| MS.gene051471 | MS.gene44915 | 0.842849 | 2.01E-58 | -1.69E-46 |
| MS.gene051663 | MS.gene44915 | 0.814207 | 1.73E-51 | -1.69E-46 |
| MS.gene051860 | MS.gene44915 | 0.805479 | 1.31E-49 | -1.69E-46 |
| MS.gene051877 | MS.gene44915 | 0.800369 | 1.49E-48 | -1.69E-46 |
| MS.gene052051 | MS.gene44915 | 0.803568 | 3.28E-49 | -1.69E-46 |
| MS.gene052078 | MS.gene44915 | 0.856076 | 4.11E-62 | -1.69E-46 |
| MS.gene052178 | MS.gene44915 | 0.823183 | 1.59E-53 | -1.69E-46 |
| MS.gene052188 | MS.gene44915 | 0.844049 | 9.62E-59 | -1.69E-46 |
| MS.gene052207 | MS.gene44915 | 0.840936 | 6.44E-58 | -1.69E-46 |
| MS.gene052287 | MS.gene44915 | 0.80259 | 5.22E-49 | -1.69E-46 |
| MS.gene052310 | MS.gene44915 | 0.838239 | 3.24E-57 | -1.69E-46 |
| MS.gene052326 | MS.gene44915 | 0.840234 | 9.83E-58 | -1.69E-46 |
| MS.gene052340 | MS.gene44915 | 0.805975 | 1.03E-49 | -1.69E-46 |
| MS.gene052485 | MS.gene44915 | 0.806557 | 7.76E-50 | -1.69E-46 |
| MS.gene052789 | MS.gene44915 | 0.808961 | 2.39E-50 | -1.69E-46 |
| MS.gene053209 | MS.gene44915 | 0.809035 | 2.31E-50 | -1.69E-46 |
| MS.gene053557 | MS.gene44915 | 0.801061 | 1.08E-48 | -1.69E-46 |
| MS.gene053836 | MS.gene44915 | 0.857202 | 1.92E-62 | -1.69E-46 |
| MS.gene053928 | MS.gene44915 | 0.812943 | 3.29E-51 | -1.69E-46 |
| MS.gene054010 | MS.gene44915 | 0.802592 | 5.21E-49 | -1.69E-46 |
| MS.gene054011 | MS.gene44915 | 0.810543 | 1.09E-50 | -1.69E-46 |
| MS.gene054123 | MS.gene44915 | 0.825575 | 4.35E-54 | -1.69E-46 |
| MS.gene054376 | MS.gene44915 | 0.824428 | 8.12E-54 | -1.69E-46 |
| MS.gene054473 | MS.gene44915 | 0.808917 | 2.45E-50 | -1.69E-46 |
| MS.gene05455 | MS.gene44915 | 0.842359 | 2.71E-58 | -1.69E-46 |
| MS.gene054802 | MS.gene44915 | 0.827434 | 1.57E-54 | -1.69E-46 |
| MS.gene054803 | MS.gene44915 | 0.828813 | 7.30E-55 | -1.69E-46 |
| MS.gene054804 | MS.gene44915 | 0.827704 | 1.35E-54 | -1.69E-46 |
| MS.gene054942 | MS.gene44915 | 0.805171 | 1.52E-49 | -1.69E-46 |
| MS.gene055116 | MS.gene44915 | 0.812522 | 4.06E-51 | -1.69E-46 |
| MS.gene055277 | MS.gene44915 | 0.859753 | 3.33E-63 | -1.69E-46 |
| MS.gene055303 | MS.gene44915 | 0.814716 | 1.34E-51 | -1.69E-46 |
| MS.gene055390 | MS.gene44915 | 0.859286 | 4.59E-63 | -1.69E-46 |
| MS.gene055422 | MS.gene44915 | 0.885546 | 7.41E-72 | -1.69E-46 |
| MS.gene055428 | MS.gene44915 | 0.874707 | 5.47E-68 | -1.69E-46 |
| MS.gene055604 | MS.gene44915 | 0.844509 | 7.24E-59 | -1.69E-46 |
| MS.gene056191 | MS.gene44915 | 0.811801 | 5.84E-51 | -1.69E-46 |
| MS.gene056314 | MS.gene44915 | 0.812814 | 3.51E-51 | -1.69E-46 |
| MS.gene056407 | MS.gene44915 | 0.833602 | 4.85E-56 | -1.69E-46 |
| MS.gene056410 | MS.gene44915 | 0.822777 | 1.98E-53 | -1.69E-46 |
| MS.gene056452 | MS.gene44915 | 0.807889 | 4.05E-50 | -1.69E-46 |
| MS.gene056612 | MS.gene44915 | 0.820019 | 8.56E-53 | -1.69E-46 |
| MS.gene056796 | MS.gene44915 | 0.816116 | 6.53E-52 | -1.69E-46 |
| MS.gene056931 | MS.gene44915 | 0.808505 | 3.00E-50 | -1.69E-46 |
| MS.gene056933 | MS.gene44915 | 0.806992 | 6.28E-50 | -1.69E-46 |
| MS.gene05702 | MS.gene44915 | 0.842418 | 2.62E-58 | -1.69E-46 |
| MS.gene057121 | MS.gene44915 | 0.851441 | 8.86E-61 | -1.69E-46 |
| MS.gene057245 | MS.gene44915 | 0.839352 | 1.67E-57 | -1.69E-46 |
| MS.gene05733 | MS.gene44915 | 0.811422 | 7.06E-51 | -1.69E-46 |
| MS.gene057439 | MS.gene44915 | 0.845961 | 2.93E-59 | -1.69E-46 |
| MS.gene057450 | MS.gene44915 | 0.81138 | 7.21E-51 | -1.69E-46 |
| MS.gene057469 | MS.gene44915 | 0.802629 | 5.12E-49 | -1.69E-46 |
| MS.gene057749 | MS.gene44915 | 0.812107 | 5.01E-51 | -1.69E-46 |
| MS.gene057823 | MS.gene44915 | 0.811941 | 5.44E-51 | -1.69E-46 |
| MS.gene058002 | MS.gene44915 | 0.863911 | 1.78E-64 | -1.69E-46 |
| MS.gene05811 | MS.gene44915 | 0.886735 | 2.64E-72 | -1.69E-46 |
| MS.gene058142 | MS.gene44915 | 0.847806 | 9.16E-60 | -1.69E-46 |
| MS.gene058233 | MS.gene44915 | 0.810486 | 1.13E-50 | -1.69E-46 |
| MS.gene058276 | MS.gene44915 | 0.826289 | 2.94E-54 | -1.69E-46 |
| MS.gene058293 | MS.gene44915 | 0.820039 | 8.47E-53 | -1.69E-46 |
| MS.gene058365 | MS.gene44915 | 0.806686 | 7.29E-50 | -1.69E-46 |
| MS.gene058660 | MS.gene44915 | 0.859202 | 4.87E-63 | -1.69E-46 |
| MS.gene05886 | MS.gene44915 | 0.869265 | 3.53E-66 | -1.69E-46 |
| MS.gene058993 | MS.gene44915 | 0.838663 | 2.52E-57 | -1.69E-46 |
| MS.gene05907 | MS.gene44915 | 0.852434 | 4.63E-61 | -1.69E-46 |
| MS.gene059326 | MS.gene44915 | 0.804429 | 2.17E-49 | -1.69E-46 |
| MS.gene059518 | MS.gene44915 | 0.866343 | 3.06E-65 | -1.69E-46 |
| MS.gene059597 | MS.gene44915 | 0.838102 | 3.51E-57 | -1.69E-46 |
| MS.gene05959 | MS.gene44915 | 0.800515 | 1.39E-48 | -1.69E-46 |
| MS.gene059676 | MS.gene44915 | 0.852043 | 5.98E-61 | -1.69E-46 |
| MS.gene059688 | MS.gene44915 | 0.822867 | 1.88E-53 | -1.69E-46 |
| MS.gene059692 | MS.gene44915 | 0.833671 | 4.66E-56 | -1.69E-46 |
| MS.gene059695 | MS.gene44915 | 0.83249 | 9.18E-56 | -1.69E-46 |
| MS.gene05984 | MS.gene44915 | 0.834424 | 3.02E-56 | -1.69E-46 |
| MS.gene059894 | MS.gene44915 | 0.837108 | 6.31E-57 | -1.69E-46 |
| MS.gene059974 | MS.gene44915 | 0.836622 | 8.40E-57 | -1.69E-46 |
| MS.gene060005 | MS.gene44915 | 0.813259 | 2.80E-51 | -1.69E-46 |
| MS.gene06037 | MS.gene44915 | 0.836819 | 7.48E-57 | -1.69E-46 |
| MS.gene060407 | MS.gene44915 | 0.831932 | 1.26E-55 | -1.69E-46 |
| MS.gene060559 | MS.gene44915 | 0.818561 | 1.84E-52 | -1.69E-46 |
| MS.gene06090 | MS.gene44915 | 0.815121 | 1.09E-51 | -1.69E-46 |
| MS.gene061049 | MS.gene44915 | 0.837962 | 3.81E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene44915 | MS.gene56742 | PPI |
| MS.gene44915 | MS.gene51249 | PPI |
| MS.gene44915 | MS.gene44409 | PPI |
| MS.gene44915 | MS.gene44411 | PPI |
| MS.gene44410 | MS.gene44915 | PPI |
| MS.gene042404 | MS.gene44915 | PPI |
| MS.gene44915 | MS.gene029233 | PPI |
| MS.gene44915 | MS.gene20554 | PPI |
| MS.gene44915 | MS.gene05395 | PPI |
| MS.gene78297 | MS.gene44915 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44915.t1 | MTR_5g035590 | 97.603 | 584 | 14 | 0 | 1 | 584 | 1 | 584 | 0.0 | 1155 |
| MS.gene44915.t1 | MTR_5g035590 | 98.771 | 407 | 5 | 0 | 178 | 584 | 1 | 407 | 0.0 | 808 |
| MS.gene44915.t1 | MTR_5g035590 | 33.784 | 296 | 183 | 5 | 187 | 474 | 122 | 412 | 5.08e-26 | 111 |
| MS.gene44915.t1 | MTR_5g035590 | 31.638 | 177 | 120 | 1 | 166 | 341 | 214 | 390 | 2.46e-17 | 85.1 |
| MS.gene44915.t1 | MTR_1g105040 | 27.509 | 269 | 195 | 0 | 204 | 472 | 217 | 485 | 5.73e-24 | 106 |
| MS.gene44915.t1 | MTR_1g105040 | 26.441 | 295 | 206 | 3 | 173 | 466 | 214 | 498 | 5.59e-22 | 100 |
| MS.gene44915.t1 | MTR_1g105040 | 27.213 | 305 | 209 | 5 | 229 | 524 | 181 | 481 | 1.34e-19 | 92.8 |
| MS.gene44915.t1 | MTR_1g105040 | 27.027 | 259 | 179 | 5 | 311 | 562 | 179 | 434 | 6.59e-16 | 81.3 |
| MS.gene44915.t1 | MTR_1g072250 | 26.316 | 399 | 256 | 7 | 153 | 543 | 134 | 502 | 2.66e-22 | 101 |
| MS.gene44915.t1 | MTR_1g072250 | 26.571 | 350 | 244 | 8 | 242 | 583 | 139 | 483 | 2.61e-18 | 88.6 |
| MS.gene44915.t1 | MTR_1g072250 | 25.000 | 320 | 236 | 3 | 151 | 467 | 192 | 510 | 2.79e-15 | 79.3 |
| MS.gene44915.t1 | MTR_3g055970 | 24.402 | 418 | 261 | 16 | 145 | 524 | 60 | 460 | 1.96e-12 | 70.9 |
| MS.gene44915.t1 | MTR_3g055970 | 23.620 | 326 | 228 | 8 | 254 | 568 | 73 | 388 | 4.32e-11 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44915.t1 | AT1G10510 | 68.697 | 591 | 167 | 5 | 2 | 584 | 3 | 583 | 0.0 | 817 |
| MS.gene44915.t1 | AT3G63130 | 27.372 | 274 | 199 | 0 | 204 | 477 | 214 | 487 | 1.07e-22 | 102 |
| MS.gene44915.t1 | AT3G63130 | 26.374 | 364 | 246 | 7 | 156 | 505 | 132 | 487 | 5.31e-22 | 100 |
| MS.gene44915.t1 | AT3G63130 | 28.777 | 278 | 191 | 5 | 308 | 583 | 206 | 478 | 1.94e-19 | 92.4 |
| MS.gene44915.t1 | AT3G63130 | 25.413 | 303 | 222 | 3 | 151 | 450 | 187 | 488 | 1.31e-18 | 89.7 |
| MS.gene44915.t1 | AT3G63130 | 26.538 | 260 | 177 | 6 | 329 | 583 | 200 | 450 | 1.19e-16 | 83.6 |
| MS.gene44915.t1 | AT3G63130 | 27.372 | 274 | 199 | 0 | 204 | 477 | 214 | 487 | 1.07e-22 | 102 |
| MS.gene44915.t1 | AT3G63130 | 26.374 | 364 | 246 | 7 | 156 | 505 | 132 | 487 | 5.31e-22 | 100 |
| MS.gene44915.t1 | AT3G63130 | 28.777 | 278 | 191 | 5 | 308 | 583 | 206 | 478 | 1.94e-19 | 92.4 |
| MS.gene44915.t1 | AT3G63130 | 25.413 | 303 | 222 | 3 | 151 | 450 | 187 | 488 | 1.31e-18 | 89.7 |
| MS.gene44915.t1 | AT3G63130 | 26.538 | 260 | 177 | 6 | 329 | 583 | 200 | 450 | 1.19e-16 | 83.6 |
| MS.gene44915.t1 | AT5G19320 | 26.182 | 275 | 202 | 1 | 204 | 477 | 219 | 493 | 1.25e-20 | 96.3 |
| MS.gene44915.t1 | AT5G19320 | 27.301 | 326 | 205 | 6 | 219 | 542 | 207 | 502 | 2.39e-17 | 85.9 |
| MS.gene44915.t1 | AT5G19320 | 25.087 | 287 | 176 | 6 | 329 | 583 | 205 | 484 | 6.87e-12 | 68.6 |
| MS.gene44915.t1 | AT5G19320 | 26.182 | 275 | 202 | 1 | 204 | 477 | 219 | 493 | 1.25e-20 | 96.3 |
| MS.gene44915.t1 | AT5G19320 | 27.301 | 326 | 205 | 6 | 219 | 542 | 207 | 502 | 2.39e-17 | 85.9 |
| MS.gene44915.t1 | AT5G19320 | 25.087 | 287 | 176 | 6 | 329 | 583 | 205 | 484 | 6.87e-12 | 68.6 |
| MS.gene44915.t1 | AT5G55540 | 23.775 | 408 | 259 | 11 | 163 | 524 | 63 | 464 | 6.85e-14 | 75.9 |
| MS.gene44915.t1 | AT5G55540 | 23.775 | 408 | 259 | 11 | 163 | 524 | 63 | 464 | 8.53e-14 | 75.5 |
Find 132 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTAATTTCCTTACCAAATT+TGG | 0.106216 | 5.1:-26235188 | MS.gene44915:CDS |
| CCTGGCACCAACCAAGTTTA+AGG | 0.149470 | 5.1:+26235706 | None:intergenic |
| ATTTAGGATTTAATGAAATA+AGG | 0.199745 | 5.1:-26235363 | MS.gene44915:CDS |
| CTGAAGACTCTTGATCTTTC+AGG | 0.215065 | 5.1:-26239671 | MS.gene44915:CDS |
| GGCAACTACGGTGGTGCTTT+GGG | 0.215447 | 5.1:-26237749 | MS.gene44915:CDS |
| AAATAGTACCTGACCAAATT+TGG | 0.219290 | 5.1:+26235175 | None:intergenic |
| GCCAAGGCCTTGGCTCCATC+TGG | 0.233421 | 5.1:+26235762 | None:intergenic |
| GCTCTAACCTCGCTAGATTT+AGG | 0.247395 | 5.1:-26235379 | MS.gene44915:CDS |
| CGCCGAAGACCGTTTGGTTT+AGG | 0.260455 | 5.1:-26243315 | MS.gene44915:CDS |
| CGAACTTCGACACGTGCTTC+TGG | 0.268208 | 5.1:-26243258 | MS.gene44915:CDS |
| TGGCAACTACGGTGGTGCTT+TGG | 0.285432 | 5.1:-26237750 | MS.gene44915:CDS |
| GCTTACAGTTATCTGGAAAT+TGG | 0.290180 | 5.1:-26242145 | MS.gene44915:intron |
| CGCCGGTTGCAATCGCTTGC+CGG | 0.291440 | 5.1:-26243375 | MS.gene44915:CDS |
| TTTAGGATTTAATGAAATAA+GGG | 0.296450 | 5.1:-26235362 | MS.gene44915:intron |
| GTCGCAGACTTTGATTGCTT+TGG | 0.305875 | 5.1:+26242099 | None:intergenic |
| CATTTGCACGGAAATTCTAT+TGG | 0.306066 | 5.1:-26237363 | MS.gene44915:CDS |
| AACGAATTGGACGGCGGTTC+CGG | 0.307180 | 5.1:+26243356 | None:intergenic |
| TGTAAATGTTCAACCATAAC+AGG | 0.310031 | 5.1:+26237105 | None:intergenic |
| TTGAGGGCAACAAGTCTATA+AGG | 0.312274 | 5.1:-26237703 | MS.gene44915:CDS |
| AACTTACTTGCGGCGGTTGC+GGG | 0.315570 | 5.1:+26243491 | None:intergenic |
| CAAATTTGTTGTCTCAGCTT+GGG | 0.325903 | 5.1:-26242023 | MS.gene44915:CDS |
| GCCACGAATGTTGCTCCGTT+TGG | 0.350546 | 5.1:-26243168 | MS.gene44915:CDS |
| AAAGGTGCTTTCTATGTTGC+TGG | 0.354044 | 5.1:-26237149 | MS.gene44915:CDS |
| CTTGGCTCCATCTGGGCCAA+TGG | 0.354591 | 5.1:+26235770 | None:intergenic |
| TTCTAGAAGCTTCGTGGTTA+AGG | 0.356055 | 5.1:-26243286 | MS.gene44915:CDS |
| GATGTGGCAGGGCGCAATTT+TGG | 0.358772 | 5.1:-26241259 | MS.gene44915:intron |
| TAACTTACTTGCGGCGGTTG+CGG | 0.363077 | 5.1:+26243490 | None:intergenic |
| GAGGTAGGGGTTTACAAGTC+TGG | 0.364818 | 5.1:-26238013 | MS.gene44915:intron |
| CTGAATCGCCGAAGACCGTT+TGG | 0.365424 | 5.1:-26243321 | MS.gene44915:CDS |
| CCCAGATGGAGCCAAGGCCT+TGG | 0.365753 | 5.1:-26235763 | MS.gene44915:CDS |
| ACAAATTTGTTGTCTCAGCT+TGG | 0.371288 | 5.1:-26242024 | MS.gene44915:CDS |
| TTGGCTCCATCTGGGCCAAT+GGG | 0.371339 | 5.1:+26235771 | None:intergenic |
| TAATACCACAATATCAGTTC+TGG | 0.377304 | 5.1:-26235562 | MS.gene44915:CDS |
| CATTCAGTTTCTGTTTAGAT+TGG | 0.379274 | 5.1:+26241986 | None:intergenic |
| CTGGCACCAACCAAGTTTAA+GGG | 0.383995 | 5.1:+26235707 | None:intergenic |
| AAAAGATCAAAAGCCTGTTA+TGG | 0.385142 | 5.1:-26237118 | MS.gene44915:CDS |
| GTCAATAACGACATTAGATA+TGG | 0.393048 | 5.1:-26236058 | MS.gene44915:intron |
| TCATGTTGTTGTTAAGTTCA+AGG | 0.398037 | 5.1:+26238136 | None:intergenic |
| CAGATGCTTTAAAGGAAAAT+CGG | 0.404566 | 5.1:-26236080 | MS.gene44915:CDS |
| TATAACTTCGGCCAAGGCCT+TGG | 0.408081 | 5.1:+26235752 | None:intergenic |
| TCAGGCGGATGCAACGCTTT+CGG | 0.409173 | 5.1:-26243211 | MS.gene44915:CDS |
| CGACACGTGCTTCTGGTTCT+CGG | 0.421744 | 5.1:-26243251 | MS.gene44915:CDS |
| GACTTTGATTGCTTTGGAGT+TGG | 0.431608 | 5.1:+26242105 | None:intergenic |
| TTCCGGCAAGCGATTGCAAC+CGG | 0.432702 | 5.1:+26243373 | None:intergenic |
| ATTGCAGGGTGCATTGTGTC+TGG | 0.434962 | 5.1:-26235428 | MS.gene44915:intron |
| GGCAATCCTGTTGGAGATGA+AGG | 0.435902 | 5.1:-26239650 | MS.gene44915:CDS |
| TCAGCTAAACAGTGCTGACT+TGG | 0.441759 | 5.1:-26238514 | MS.gene44915:CDS |
| AGACCGTTTGGTTTAGGAGC+AGG | 0.442226 | 5.1:-26243309 | MS.gene44915:CDS |
| GGAATTCGTTCTTTGATGAC+TGG | 0.448428 | 5.1:-26237333 | MS.gene44915:CDS |
| GCAACTACGGTGGTGCTTTG+GGG | 0.449523 | 5.1:-26237748 | MS.gene44915:CDS |
| TTTATGCAGATAGGAGCAAA+AGG | 0.450654 | 5.1:-26235615 | MS.gene44915:intron |
| TCAATCCTTGTGAAGCAGAC+TGG | 0.451465 | 5.1:+26242067 | None:intergenic |
| CAAGCGATTGCAACCGGCGG+CGG | 0.452096 | 5.1:+26243379 | None:intergenic |
| CTCCTAAACCAAACGGTCTT+CGG | 0.454472 | 5.1:+26243313 | None:intergenic |
| TTTCCTTACCAAATTTGGTC+AGG | 0.455058 | 5.1:-26235183 | None:intergenic |
| CTGCTTCACAAGGATTGAAA+TGG | 0.456014 | 5.1:-26242062 | MS.gene44915:CDS |
| GGAAATAACATTCATGCTGT+TGG | 0.457502 | 5.1:-26235944 | MS.gene44915:CDS |
| GGAAATTCTATTGGAGATGA+AGG | 0.458044 | 5.1:-26237354 | MS.gene44915:CDS |
| TGCAACGCTTTCGGTTGCTT+CGG | 0.462705 | 5.1:-26243202 | MS.gene44915:CDS |
| CCTTAAACTTGGTTGGTGCC+AGG | 0.465386 | 5.1:-26235706 | MS.gene44915:intron |
| TTAGCTCCTTCATCTCCAAC+AGG | 0.469951 | 5.1:+26239644 | None:intergenic |
| TCAGCTTGGGGCGAAGATTG+AGG | 0.471276 | 5.1:-26242010 | MS.gene44915:CDS |
| GTTCTGGACTTACGAGCAAA+TGG | 0.472854 | 5.1:-26235546 | MS.gene44915:CDS |
| CCAAGGCCTTGGCTCCATCT+GGG | 0.475782 | 5.1:+26235763 | None:intergenic |
| ATTCTTTCTTGCGGAGAGCT+TGG | 0.477259 | 5.1:-26241224 | MS.gene44915:CDS |
| CAGCTTGGGGCGAAGATTGA+GGG | 0.477389 | 5.1:-26242009 | MS.gene44915:CDS |
| GAGAGTTTATCGCGAGTCTC+AGG | 0.483584 | 5.1:-26243229 | MS.gene44915:CDS |
| TGTAGCTTCGTCTCCGCCGC+CGG | 0.485091 | 5.1:-26243392 | MS.gene44915:intron |
| GAACTTAGTTATAATCCCAT+TGG | 0.491304 | 5.1:-26235786 | MS.gene44915:CDS |
| GCAAATGGAATAACTGCTGC+TGG | 0.492022 | 5.1:-26239734 | MS.gene44915:CDS |
| GAGTCGGAAGGATAACGAAT+TGG | 0.496132 | 5.1:+26243343 | None:intergenic |
| TCCATGGAACTTTATAACTT+CGG | 0.498470 | 5.1:+26235740 | None:intergenic |
| AACAACTCATTGACAGCTAA+AGG | 0.500253 | 5.1:-26237167 | MS.gene44915:CDS |
| GCCGAAGTTATAAAGTTCCA+TGG | 0.510009 | 5.1:-26235741 | MS.gene44915:CDS |
| GTGTGAGTAAAGAGAGAGAG+TGG | 0.511008 | 5.1:+26243513 | None:intergenic |
| CAACTACGGTGGTGCTTTGG+GGG | 0.511771 | 5.1:-26237747 | MS.gene44915:CDS |
| TTCGATTCAAGTGGCAACTA+CGG | 0.512684 | 5.1:-26237761 | MS.gene44915:intron |
| AGTGGAAGTGTTTAAAGACA+TGG | 0.513176 | 5.1:+26243531 | None:intergenic |
| CAGCTAAACAGTGCTGACTT+GGG | 0.514024 | 5.1:-26238513 | MS.gene44915:CDS |
| ATCTTTCAAAACTTTAGCAA+CGG | 0.514052 | 5.1:+26235913 | None:intergenic |
| TGCATTGGCTAAAGCACTTG+AGG | 0.518489 | 5.1:-26237720 | MS.gene44915:CDS |
| TATAATCCCATTGGCCCAGA+TGG | 0.518540 | 5.1:-26235777 | MS.gene44915:CDS |
| TAGGGGTTTACAAGTCTGGC+TGG | 0.518877 | 5.1:-26238009 | MS.gene44915:CDS |
| AAGGTGCTTTCTATGTTGCT+GGG | 0.521393 | 5.1:-26237148 | MS.gene44915:CDS |
| GTTTAGCTGAAGCTTCTCAA+TGG | 0.527253 | 5.1:+26238527 | None:intergenic |
| TCGTCAAACTCACAGGATGA+TGG | 0.528672 | 5.1:-26235278 | MS.gene44915:intron |
| TACAGTTATCTGGAAATTGG+TGG | 0.530008 | 5.1:-26242142 | MS.gene44915:intron |
| TGAGGGCAACAAGTCTATAA+GGG | 0.535265 | 5.1:-26237702 | MS.gene44915:intron |
| GGAGAGCTTGGCATTTAACC+AGG | 0.535384 | 5.1:-26241212 | MS.gene44915:intron |
| AACATTCATGCTGTTGGAGT+TGG | 0.536123 | 5.1:-26235938 | MS.gene44915:CDS |
| AAATTTGTTGTCTCAGCTTG+GGG | 0.537078 | 5.1:-26242022 | MS.gene44915:CDS |
| GATCTTTCAGGCAATCCTGT+TGG | 0.539593 | 5.1:-26239659 | MS.gene44915:CDS |
| GCATTGGCTAAAGCACTTGA+GGG | 0.543471 | 5.1:-26237719 | MS.gene44915:CDS |
| AGCTAAACAGTGCTGACTTG+GGG | 0.548080 | 5.1:-26238512 | MS.gene44915:CDS |
| TGAAGGATTATTCTTTCTTG+CGG | 0.550404 | 5.1:-26241233 | MS.gene44915:CDS |
| AACATTTACATGAATGACAT+TGG | 0.551290 | 5.1:-26237092 | MS.gene44915:CDS |
| TTATGCAGATAGGAGCAAAA+GGG | 0.551711 | 5.1:-26235614 | MS.gene44915:intron |
| AAACTTACACTTCTAGACAT+AGG | 0.558147 | 5.1:-26237191 | MS.gene44915:CDS |
| GTACCTGACCAAATTTGGTA+AGG | 0.565710 | 5.1:+26235180 | None:intergenic |
| AGCAGGTTCTAGAAGCTTCG+TGG | 0.572221 | 5.1:-26243292 | MS.gene44915:CDS |
| AGTGCTAAGAACACTCCAAA+CGG | 0.573474 | 5.1:+26243153 | None:intergenic |
| TAGCTGAAGCTTCTCAATGG+TGG | 0.574007 | 5.1:+26238530 | None:intergenic |
| TCCAAACGGAGCAACATTCG+TGG | 0.576544 | 5.1:+26243167 | None:intergenic |
| GAACCTGCTCCTAAACCAAA+CGG | 0.577145 | 5.1:+26243306 | None:intergenic |
| TGTTGGAGATGAAGGAGCTA+AGG | 0.580011 | 5.1:-26239642 | MS.gene44915:intron |
| CATTGGCCCAGATGGAGCCA+AGG | 0.586640 | 5.1:-26235769 | MS.gene44915:CDS |
| ATTAAATCCTAAATCTAGCG+AGG | 0.589016 | 5.1:+26235372 | None:intergenic |
| GAACTTTATAACTTCGGCCA+AGG | 0.589359 | 5.1:+26235746 | None:intergenic |
| GAAAGCCAGTCTGCTTCACA+AGG | 0.590388 | 5.1:-26242072 | MS.gene44915:CDS |
| GGTCTTCGGCGATTCAGAGT+CGG | 0.599218 | 5.1:+26243327 | None:intergenic |
| AATGTGAAAACCCTTAAACT+TGG | 0.599464 | 5.1:-26235717 | MS.gene44915:CDS |
| TGAAAACCCTTAAACTTGGT+TGG | 0.600564 | 5.1:-26235713 | MS.gene44915:CDS |
| GTGTTCTTAGCACTTACCAT+CGG | 0.600836 | 5.1:-26243144 | MS.gene44915:intron |
| GTGTCTATGTGACATACTGA+TGG | 0.618276 | 5.1:-26238559 | MS.gene44915:CDS |
| GCTGTCACATCTCTAAACAT+TGG | 0.622840 | 5.1:-26235212 | MS.gene44915:CDS |
| TATGCAGATAGGAGCAAAAG+GGG | 0.628011 | 5.1:-26235613 | MS.gene44915:intron |
| CGGCAAGCGATTGCAACCGG+CGG | 0.628871 | 5.1:+26243376 | None:intergenic |
| ACTGGATTAACGTCACATAG+AGG | 0.632787 | 5.1:-26237315 | MS.gene44915:intron |
| CATGAATGACATTGGTGATG+AGG | 0.633304 | 5.1:-26237084 | MS.gene44915:intron |
| GCTAAACAGTGCTGACTTGG+GGG | 0.640733 | 5.1:-26238511 | MS.gene44915:CDS |
| AGTTTATCGCGAGTCTCAGG+CGG | 0.642766 | 5.1:-26243226 | MS.gene44915:CDS |
| CGGAAGGATAACGAATTGGA+CGG | 0.651382 | 5.1:+26243347 | None:intergenic |
| GATTCAAGTGGCAACTACGG+TGG | 0.655517 | 5.1:-26237758 | MS.gene44915:intron |
| GGACAGGAACTACATTTGCA+CGG | 0.660249 | 5.1:-26237375 | MS.gene44915:intron |
| ATGAGTTTGCTCGAGAACTG+AGG | 0.662789 | 5.1:-26241966 | MS.gene44915:CDS |
| TAAGTCCAGAACTGATATTG+TGG | 0.673601 | 5.1:+26235557 | None:intergenic |
| TTCGGCGATTCAGAGTCGGA+AGG | 0.683412 | 5.1:+26243331 | None:intergenic |
| AGTTAGTAACTTACTTGCGG+CGG | 0.686730 | 5.1:+26243484 | None:intergenic |
| ATATACTATATAGACTGCAG+AGG | 0.689045 | 5.1:-26239771 | MS.gene44915:intron |
| TGTCTATGTGACATACTGAT+GGG | 0.736138 | 5.1:-26238558 | MS.gene44915:CDS |
| AAGGATAACGAATTGGACGG+CGG | 0.749420 | 5.1:+26243350 | None:intergenic |
| AAAAGGGGCAGAGTTCATTG+CGG | 0.749883 | 5.1:-26235598 | MS.gene44915:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 26235184 | 26243551 | 26235184 | ID=MS.gene44915 |
| chr5.1 | mRNA | 26235184 | 26243551 | 26235184 | ID=MS.gene44915.t1;Parent=MS.gene44915 |
| chr5.1 | exon | 26243498 | 26243551 | 26243498 | ID=MS.gene44915.t1.exon1;Parent=MS.gene44915.t1 |
| chr5.1 | CDS | 26243498 | 26243551 | 26243498 | ID=cds.MS.gene44915.t1;Parent=MS.gene44915.t1 |
| chr5.1 | exon | 26243145 | 26243409 | 26243145 | ID=MS.gene44915.t1.exon2;Parent=MS.gene44915.t1 |
| chr5.1 | CDS | 26243145 | 26243409 | 26243145 | ID=cds.MS.gene44915.t1;Parent=MS.gene44915.t1 |
| chr5.1 | exon | 26241941 | 26242159 | 26241941 | ID=MS.gene44915.t1.exon3;Parent=MS.gene44915.t1 |
| chr5.1 | CDS | 26241941 | 26242159 | 26241941 | ID=cds.MS.gene44915.t1;Parent=MS.gene44915.t1 |
| chr5.1 | exon | 26241213 | 26241271 | 26241213 | ID=MS.gene44915.t1.exon4;Parent=MS.gene44915.t1 |
| chr5.1 | CDS | 26241213 | 26241271 | 26241213 | ID=cds.MS.gene44915.t1;Parent=MS.gene44915.t1 |
| chr5.1 | exon | 26239643 | 26239780 | 26239643 | ID=MS.gene44915.t1.exon5;Parent=MS.gene44915.t1 |
| chr5.1 | CDS | 26239643 | 26239780 | 26239643 | ID=cds.MS.gene44915.t1;Parent=MS.gene44915.t1 |
| chr5.1 | exon | 26238506 | 26238580 | 26238506 | ID=MS.gene44915.t1.exon6;Parent=MS.gene44915.t1 |
| chr5.1 | CDS | 26238506 | 26238580 | 26238506 | ID=cds.MS.gene44915.t1;Parent=MS.gene44915.t1 |
| chr5.1 | exon | 26238125 | 26238208 | 26238125 | ID=MS.gene44915.t1.exon7;Parent=MS.gene44915.t1 |
| chr5.1 | CDS | 26238125 | 26238208 | 26238125 | ID=cds.MS.gene44915.t1;Parent=MS.gene44915.t1 |
| chr5.1 | exon | 26237967 | 26238028 | 26237967 | ID=MS.gene44915.t1.exon8;Parent=MS.gene44915.t1 |
| chr5.1 | CDS | 26237967 | 26238028 | 26237967 | ID=cds.MS.gene44915.t1;Parent=MS.gene44915.t1 |
| chr5.1 | exon | 26237703 | 26237772 | 26237703 | ID=MS.gene44915.t1.exon9;Parent=MS.gene44915.t1 |
| chr5.1 | CDS | 26237703 | 26237772 | 26237703 | ID=cds.MS.gene44915.t1;Parent=MS.gene44915.t1 |
| chr5.1 | exon | 26237316 | 26237391 | 26237316 | ID=MS.gene44915.t1.exon10;Parent=MS.gene44915.t1 |
| chr5.1 | CDS | 26237316 | 26237391 | 26237316 | ID=cds.MS.gene44915.t1;Parent=MS.gene44915.t1 |
| chr5.1 | exon | 26237085 | 26237215 | 26237085 | ID=MS.gene44915.t1.exon11;Parent=MS.gene44915.t1 |
| chr5.1 | CDS | 26237085 | 26237215 | 26237085 | ID=cds.MS.gene44915.t1;Parent=MS.gene44915.t1 |
| chr5.1 | exon | 26236059 | 26236118 | 26236059 | ID=MS.gene44915.t1.exon12;Parent=MS.gene44915.t1 |
| chr5.1 | CDS | 26236059 | 26236118 | 26236059 | ID=cds.MS.gene44915.t1;Parent=MS.gene44915.t1 |
| chr5.1 | exon | 26235895 | 26235969 | 26235895 | ID=MS.gene44915.t1.exon13;Parent=MS.gene44915.t1 |
| chr5.1 | CDS | 26235895 | 26235969 | 26235895 | ID=cds.MS.gene44915.t1;Parent=MS.gene44915.t1 |
| chr5.1 | exon | 26235707 | 26235811 | 26235707 | ID=MS.gene44915.t1.exon14;Parent=MS.gene44915.t1 |
| chr5.1 | CDS | 26235707 | 26235811 | 26235707 | ID=cds.MS.gene44915.t1;Parent=MS.gene44915.t1 |
| chr5.1 | exon | 26235533 | 26235628 | 26235533 | ID=MS.gene44915.t1.exon15;Parent=MS.gene44915.t1 |
| chr5.1 | CDS | 26235533 | 26235628 | 26235533 | ID=cds.MS.gene44915.t1;Parent=MS.gene44915.t1 |
| chr5.1 | exon | 26235363 | 26235443 | 26235363 | ID=MS.gene44915.t1.exon16;Parent=MS.gene44915.t1 |
| chr5.1 | CDS | 26235363 | 26235443 | 26235363 | ID=cds.MS.gene44915.t1;Parent=MS.gene44915.t1 |
| chr5.1 | exon | 26235184 | 26235285 | 26235184 | ID=MS.gene44915.t1.exon17;Parent=MS.gene44915.t1 |
| chr5.1 | CDS | 26235184 | 26235285 | 26235184 | ID=cds.MS.gene44915.t1;Parent=MS.gene44915.t1 |
| Gene Sequence |
| Protein sequence |
