AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene48401


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 66 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCCTTCTCAGTATATTAAT+TGG 0.148489 8.3:-18246206 MS.gene48401:CDS
TTTCCTGAATTCTTGTAATT+TGG 0.171860 8.3:+18246410 None:intergenic
GGTGTTAACTTTGCTGTTGC+TGG 0.263157 8.3:-18247050 MS.gene48401:CDS
CAGTGTCTCAGGAGCTTTAC+AGG 0.269526 8.3:-18246778 MS.gene48401:intron
GCTGAAGGTCCAAGTGGTTT+TGG 0.306391 8.3:-18247227 MS.gene48401:CDS
AATGATTATGCTTACACTCT+TGG 0.315837 8.3:-18246843 MS.gene48401:CDS
TAAACCATGAATTCTTTGTT+AGG 0.329985 8.3:-18247016 MS.gene48401:CDS
AAAAGATCAAATGATACTTT+TGG 0.334241 8.3:-18247071 MS.gene48401:CDS
TCTTACCGAAGCTATGTATA+AGG 0.339713 8.3:-18246172 MS.gene48401:CDS
TATTCCTAACAAAGAATTCA+TGG 0.348303 8.3:+18247012 None:intergenic
CAGTCTCAGAAGATACTATA+AGG 0.400671 8.3:-18246815 MS.gene48401:CDS
CCCTTCTCAGTATATTAATT+GGG 0.408026 8.3:-18246205 MS.gene48401:CDS
GTGCAAGGTCATCCACCAAC+AGG 0.409203 8.3:-18246540 MS.gene48401:CDS
TTCAAGAAAATTTACGCCTT+CGG 0.409856 8.3:-18247284 MS.gene48401:CDS
ATGAAGAATCCAAGCAAGTA+TGG 0.412451 8.3:-18246330 MS.gene48401:CDS
TAAGGTTGTGATTGTTGCTT+AGG 0.412894 8.3:+18246442 None:intergenic
GTTTCTAATATGTTTCTTCA+AGG 0.421614 8.3:-18246147 MS.gene48401:CDS
CTTGCAATGTCCAGTGTCTC+AGG 0.422400 8.3:-18246789 MS.gene48401:CDS
CCTAAGTCGTCTCTGTCATC+TGG 0.426751 8.3:+18246486 None:intergenic
AAGCCAAATTACAAGAATTC+AGG 0.428938 8.3:-18246413 MS.gene48401:CDS
GTAATTTGGCTTGCAGCATA+AGG 0.433254 8.3:+18246424 None:intergenic
TTCAGTGTCTGCTGTGGATC+AGG 0.434569 8.3:-18246294 MS.gene48401:CDS
TCTTTGAATCCATACTTGCT+TGG 0.457241 8.3:+18246321 None:intergenic
TCCATCAGAATATCTGTTTG+TGG 0.461336 8.3:+18247160 None:intergenic
GCAAACACAGTAAAGTTGTA+AGG 0.470362 8.3:+18246264 None:intergenic
CTAAATACATTGTCAGTGTA+AGG 0.474491 8.3:+18246514 None:intergenic
CTGTAAAGCTCCTGAGACAC+TGG 0.475224 8.3:+18246779 None:intergenic
CTAGTGCAGACAGTAGCATT+AGG 0.483410 8.3:+18246228 None:intergenic
TTTACTGTGTTTGCTACTTG+TGG 0.486375 8.3:-18246255 MS.gene48401:CDS
CCCAATTAATATACTGAGAA+GGG 0.489096 8.3:+18246205 None:intergenic
ATTGTGGTTTAATAAGTACT+TGG 0.491945 8.3:-18246946 MS.gene48401:CDS
AACAACCTTATACATAGCTT+CGG 0.507341 8.3:+18246167 None:intergenic
ACCTTGCACAACTAGGTACT+TGG 0.509796 8.3:+18246554 None:intergenic
CTATTCAAACTCAGATATTG+TGG 0.510682 8.3:-18246962 MS.gene48401:CDS
AATCAACTCCTTGACAACCT+TGG 0.512025 8.3:+18246919 None:intergenic
TTGAAGAAAGTTGTTCCATA+TGG 0.515073 8.3:+18247188 None:intergenic
GATACATGGCCAAAACCACT+TGG 0.515353 8.3:+18247218 None:intergenic
TGACGATAAGGAGGAAGATA+AGG 0.521221 8.3:+18247098 None:intergenic
AGTGTAAGGCACCCTGTTGG+TGG 0.525307 8.3:+18246528 None:intergenic
TCTCAGTATATTAATTGGGA+TGG 0.526757 8.3:-18246201 MS.gene48401:CDS
TTTGCAGTCATTGCTTGAGA+AGG 0.530230 8.3:-18246580 MS.gene48401:intron
CCAGATGACAGAGACGACTT+AGG 0.531180 8.3:-18246486 MS.gene48401:CDS
TTGCAGTCATTGCTTGAGAA+GGG 0.533529 8.3:-18246579 MS.gene48401:intron
TCCCAATTAATATACTGAGA+AGG 0.543751 8.3:+18246204 None:intergenic
CATGTATCAAACTCACCATA+TGG 0.543972 8.3:-18247203 MS.gene48401:CDS
GCATAAACTATGACTGCATG+TGG 0.551677 8.3:+18246381 None:intergenic
CAGAATATCTGTTTGTGGAA+TGG 0.551682 8.3:+18247165 None:intergenic
AATAAGTACTTGGAGAGCCA+AGG 0.559798 8.3:-18246936 MS.gene48401:CDS
TTGGAGAGCCAAGGTTGTCA+AGG 0.574397 8.3:-18246927 MS.gene48401:CDS
GTGGATGACCTTGCACAACT+AGG 0.575276 8.3:+18246547 None:intergenic
TTGGATTCTTCATCACAGTA+CGG 0.581211 8.3:+18246340 None:intergenic
CATATGGTGAGTTTGATACA+TGG 0.583710 8.3:+18247204 None:intergenic
TCCACAAACAGATATTCTGA+TGG 0.587630 8.3:-18247161 MS.gene48401:CDS
GGAGACTCTTTCACAGACAC+AGG 0.589716 8.3:-18247263 MS.gene48401:CDS
GGCAACACACACAACGCTGA+AGG 0.600278 8.3:-18247242 MS.gene48401:CDS
GTCTGTGAAAGAGTCTCCGA+AGG 0.622353 8.3:+18247268 None:intergenic
AAGTCGTCTCTGTCATCTGG+AGG 0.624671 8.3:+18246489 None:intergenic
GCCAAGTACCTAGTTGTGCA+AGG 0.625395 8.3:-18246555 MS.gene48401:CDS
GTCAGTGTAAGGCACCCTGT+TGG 0.629703 8.3:+18246525 None:intergenic
TGCAAGGTCATCCACCAACA+GGG 0.656181 8.3:-18246539 MS.gene48401:CDS
TTGGAAAACAAAGAGAGACA+TGG 0.662787 8.3:-18246093 MS.gene48401:CDS
AACACAGTAAAGTTGTAAGG+AGG 0.674046 8.3:+18246267 None:intergenic
GATATGTTCAGTGTCTGCTG+TGG 0.678417 8.3:-18246300 MS.gene48401:CDS
CACAACGCTGAAGGTCCAAG+TGG 0.686660 8.3:-18247233 MS.gene48401:CDS
ATCTGAGTTTGAATAGACTG+AGG 0.688837 8.3:+18246969 None:intergenic
CATAAACTATGACTGCATGT+GGG 0.692524 8.3:+18246382 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATATGGTTTATTTGT+TGG - chr8.3:18246710-18246729 MS.gene48401:intron 15.0%
!! AAAAATGGGAGTTATATATA+TGG - chr8.3:18246698-18246717 MS.gene48401:intron 20.0%
!!! AAAAGATCAAATGATACTTT+TGG - chr8.3:18246343-18246362 MS.gene48401:CDS 20.0%
!!! TTTTCCAACAAAAAATCAAA+TGG + chr8.3:18247309-18247328 None:intergenic 20.0%
! AAGATTAGTGCACATAAAAA+TGG - chr8.3:18246683-18246702 MS.gene48401:intron 25.0%
! AGATTAGTGCACATAAAAAT+GGG - chr8.3:18246684-18246703 MS.gene48401:intron 25.0%
! ATTGTGGTTTAATAAGTACT+TGG - chr8.3:18246468-18246487 MS.gene48401:CDS 25.0%
! GTTTCTAATATGTTTCTTCA+AGG - chr8.3:18247267-18247286 MS.gene48401:CDS 25.0%
! TAAACCATGAATTCTTTGTT+AGG - chr8.3:18246398-18246417 MS.gene48401:CDS 25.0%
! TATTCCTAACAAAGAATTCA+TGG + chr8.3:18246405-18246424 None:intergenic 25.0%
! TTTCCTGAATTCTTGTAATT+TGG + chr8.3:18247007-18247026 None:intergenic 25.0%
!!! ATTTTGATGAGACTCTTTTT+TGG - chr8.3:18246530-18246549 MS.gene48401:CDS 25.0%
AACAACCTTATACATAGCTT+CGG + chr8.3:18247250-18247269 None:intergenic 30.0%
AAGCCAAATTACAAGAATTC+AGG - chr8.3:18247001-18247020 MS.gene48401:CDS 30.0%
AATGATTATGCTTACACTCT+TGG - chr8.3:18246571-18246590 MS.gene48401:CDS 30.0%
ACAAAAAATCAAATGGAGGT+TGG + chr8.3:18247302-18247321 None:intergenic 30.0%
CCCAATTAATATACTGAGAA+GGG + chr8.3:18247212-18247231 None:intergenic 30.0%
CCCTTCTCAGTATATTAATT+GGG - chr8.3:18247209-18247228 MS.gene48401:CDS 30.0%
CTAAATACATTGTCAGTGTA+AGG + chr8.3:18246903-18246922 None:intergenic 30.0%
CTATTCAAACTCAGATATTG+TGG - chr8.3:18246452-18246471 MS.gene48401:CDS 30.0%
GAAAAAAAATCTCTAGACCA+AGG + chr8.3:18246743-18246762 None:intergenic 30.0%
TCCAACAAAAAATCAAATGG+AGG + chr8.3:18247306-18247325 None:intergenic 30.0%
TCCCAATTAATATACTGAGA+AGG + chr8.3:18247213-18247232 None:intergenic 30.0%
TCTCAGTATATTAATTGGGA+TGG - chr8.3:18247213-18247232 MS.gene48401:CDS 30.0%
TTCAAGAAAATTTACGCCTT+CGG - chr8.3:18246130-18246149 MS.gene48401:CDS 30.0%
TTGAAGAAAGTTGTTCCATA+TGG + chr8.3:18246229-18246248 None:intergenic 30.0%
! AAGGCGTAAATTTTCTTGAA+AGG + chr8.3:18246130-18246149 None:intergenic 30.0%
! TATGGTTTATTTGTTGGTCT+TGG - chr8.3:18246716-18246735 MS.gene48401:intron 30.0%
!!! ACCTCCATTTGATTTTTTGT+TGG - chr8.3:18247302-18247321 MS.gene48401:CDS 30.0%
!!! CTTTTTTGGTTTGGTGAAAT+TGG - chr8.3:18246544-18246563 MS.gene48401:CDS 30.0%
AACACAGTAAAGTTGTAAGG+AGG + chr8.3:18247150-18247169 None:intergenic 35.0%
ATCTGAGTTTGAATAGACTG+AGG + chr8.3:18246448-18246467 None:intergenic 35.0%
ATGAAGAATCCAAGCAAGTA+TGG - chr8.3:18247084-18247103 MS.gene48401:CDS 35.0%
CAGAATATCTGTTTGTGGAA+TGG + chr8.3:18246252-18246271 None:intergenic 35.0%
CAGTCTCAGAAGATACTATA+AGG - chr8.3:18246599-18246618 MS.gene48401:intron 35.0%
CATAAACTATGACTGCATGT+GGG + chr8.3:18247035-18247054 None:intergenic 35.0%
CATATGGTGAGTTTGATACA+TGG + chr8.3:18246213-18246232 None:intergenic 35.0%
CATGTATCAAACTCACCATA+TGG - chr8.3:18246211-18246230 MS.gene48401:CDS 35.0%
GCAAACACAGTAAAGTTGTA+AGG + chr8.3:18247153-18247172 None:intergenic 35.0%
GCCCTTCTCAGTATATTAAT+TGG - chr8.3:18247208-18247227 MS.gene48401:CDS 35.0%
TCCACAAACAGATATTCTGA+TGG - chr8.3:18246253-18246272 MS.gene48401:CDS 35.0%
TCCATCAGAATATCTGTTTG+TGG + chr8.3:18246257-18246276 None:intergenic 35.0%
TCTTACCGAAGCTATGTATA+AGG - chr8.3:18247242-18247261 MS.gene48401:CDS 35.0%
TCTTTGAATCCATACTTGCT+TGG + chr8.3:18247096-18247115 None:intergenic 35.0%
TTGGAAAACAAAGAGAGACA+TGG - chr8.3:18247321-18247340 MS.gene48401:CDS 35.0%
TTGGATTCTTCATCACAGTA+CGG + chr8.3:18247077-18247096 None:intergenic 35.0%
! GATCTTTTGATGTGACGATA+AGG + chr8.3:18246331-18246350 None:intergenic 35.0%
! TAAGGTTGTGATTGTTGCTT+AGG + chr8.3:18246975-18246994 None:intergenic 35.0%
! TTTACTGTGTTTGCTACTTG+TGG - chr8.3:18247159-18247178 MS.gene48401:CDS 35.0%
!!! GATGAGACTCTTTTTTGGTT+TGG - chr8.3:18246535-18246554 MS.gene48401:CDS 35.0%
AATAAGTACTTGGAGAGCCA+AGG - chr8.3:18246478-18246497 MS.gene48401:CDS 40.0%
AATCAACTCCTTGACAACCT+TGG + chr8.3:18246498-18246517 None:intergenic 40.0%
GCATAAACTATGACTGCATG+TGG + chr8.3:18247036-18247055 None:intergenic 40.0%
TGACGATAAGGAGGAAGATA+AGG + chr8.3:18246319-18246338 None:intergenic 40.0%
TTGCAGTCATTGCTTGAGAA+GGG - chr8.3:18246835-18246854 MS.gene48401:CDS 40.0%
TTTGCAGTCATTGCTTGAGA+AGG - chr8.3:18246834-18246853 MS.gene48401:CDS 40.0%
! CATTGATAATTCGCGAGGAT+CGG + chr8.3:18246776-18246795 None:intergenic 40.0%
! CTTTTGATGTGACGATAAGG+AGG + chr8.3:18246328-18246347 None:intergenic 40.0%
!! GAAAGCATTGATAATTCGCG+AGG + chr8.3:18246781-18246800 None:intergenic 40.0%
!! GTAATTTGGCTTGCAGCATA+AGG + chr8.3:18246993-18247012 None:intergenic 40.0%
!! TATTTGTTGGTCTTGGACCT+TGG - chr8.3:18246723-18246742 MS.gene48401:intron 40.0%
ACCTTGCACAACTAGGTACT+TGG + chr8.3:18246863-18246882 None:intergenic 45.0%
CTAGTGCAGACAGTAGCATT+AGG + chr8.3:18247189-18247208 None:intergenic 45.0%
GATACATGGCCAAAACCACT+TGG + chr8.3:18246199-18246218 None:intergenic 45.0%
GATATGTTCAGTGTCTGCTG+TGG - chr8.3:18247114-18247133 MS.gene48401:CDS 45.0%
!! GGTGTTAACTTTGCTGTTGC+TGG - chr8.3:18246364-18246383 MS.gene48401:CDS 45.0%
AAGTCGTCTCTGTCATCTGG+AGG + chr8.3:18246928-18246947 None:intergenic 50.0%
CAGTGTCTCAGGAGCTTTAC+AGG - chr8.3:18246636-18246655 MS.gene48401:intron 50.0%
CCAGATGACAGAGACGACTT+AGG - chr8.3:18246928-18246947 MS.gene48401:CDS 50.0%
CCTAAGTCGTCTCTGTCATC+TGG + chr8.3:18246931-18246950 None:intergenic 50.0%
CTGTAAAGCTCCTGAGACAC+TGG + chr8.3:18246638-18246657 None:intergenic 50.0%
CTTGCAATGTCCAGTGTCTC+AGG - chr8.3:18246625-18246644 MS.gene48401:intron 50.0%
GCCAAGTACCTAGTTGTGCA+AGG - chr8.3:18246859-18246878 MS.gene48401:CDS 50.0%
GGAGACTCTTTCACAGACAC+AGG - chr8.3:18246151-18246170 MS.gene48401:CDS 50.0%
GTCTGTGAAAGAGTCTCCGA+AGG + chr8.3:18246149-18246168 None:intergenic 50.0%
GTGGATGACCTTGCACAACT+AGG + chr8.3:18246870-18246889 None:intergenic 50.0%
TGCAAGGTCATCCACCAACA+GGG - chr8.3:18246875-18246894 MS.gene48401:CDS 50.0%
TTCAGTGTCTGCTGTGGATC+AGG - chr8.3:18247120-18247139 MS.gene48401:CDS 50.0%
TTGGAGAGCCAAGGTTGTCA+AGG - chr8.3:18246487-18246506 MS.gene48401:CDS 50.0%
! GCTGAAGGTCCAAGTGGTTT+TGG - chr8.3:18246187-18246206 MS.gene48401:CDS 50.0%
CACAACGCTGAAGGTCCAAG+TGG - chr8.3:18246181-18246200 MS.gene48401:CDS 55.0%
GGCAACACACACAACGCTGA+AGG - chr8.3:18246172-18246191 MS.gene48401:CDS 55.0%
GTGCAAGGTCATCCACCAAC+AGG - chr8.3:18246874-18246893 MS.gene48401:CDS 55.0%
! AGTGTAAGGCACCCTGTTGG+TGG + chr8.3:18246889-18246908 None:intergenic 55.0%
! GTCAGTGTAAGGCACCCTGT+TGG + chr8.3:18246892-18246911 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 18246089 18247347 18246089 ID=MS.gene48401
chr8.3 mRNA 18246089 18247347 18246089 ID=MS.gene48401.t1;Parent=MS.gene48401
chr8.3 exon 18246779 18247347 18246779 ID=MS.gene48401.t1.exon1;Parent=MS.gene48401.t1
chr8.3 CDS 18246779 18247347 18246779 ID=cds.MS.gene48401.t1;Parent=MS.gene48401.t1
chr8.3 exon 18246089 18246595 18246089 ID=MS.gene48401.t1.exon2;Parent=MS.gene48401.t1
chr8.3 CDS 18246089 18246595 18246089 ID=cds.MS.gene48401.t1;Parent=MS.gene48401.t1
Gene Sequence

>MS.gene48401

CTTCTTCAGCTACATCAACTACAGAACAACTTTCAAGACCTTTCAAGAAAATTTACGCCTTCGGAGACTCTTTCACAGACACAGGCAACACACACAACGCTGAAGGTCCAAGTGGTTTTGGCCATGTATCAAACTCACCATATGGAACAACTTTCTTCAACCATTCCACAAACAGATATTCTGATGGAAGACTTGTTATTGACTTTGTCACAGAATCACTTTCTTTGCCTTATCTTCCTCCTTATCGTCACATCAAAAGATCAAATGATACTTTTGGTGTTAACTTTGCTGTTGCTGGTTCAACAGCTATAAACCATGAATTCTTTGTTAGGAATAATCTTTCTCTTGACATCACTCCTCAGTCTATTCAAACTCAGATATTGTGGTTTAATAAGTACTTGGAGAGCCAAGGTTGTCAAGGAGTTGATTCAAAGTGTAAAGATTTTGATGAGACTCTTTTTTGGTTTGGTGAAATTGGTGTTAATGATTATGCTTACACTCTTGGATCTACAGTCTCAGAAGATACTATAAGGAAGCTTGCAATGTCCAGTGTCTCAGGAGCTTTACAGGTAATATACTATTTGATTCATGTTTAAGATTAGTGCACATAAAAATGGGAGTTATATATATGGTTTATTTGTTGGTCTTGGACCTTGGTCTAGAGATTTTTTTTCATTCTTACAACCGATCCTCGCGAATTATCAATGCTTTCATATATTAACTGTTTGTTATATTCTTTGTTAATTTTGCAGTCATTGCTTGAGAAGGGTGCCAAGTACCTAGTTGTGCAAGGTCATCCACCAACAGGGTGCCTTACACTGACAATGTATTTAGCTCCTCCAGATGACAGAGACGACTTAGGATGTGTGAAAAGTGCTAACGACCTAAGCAACAATCACAACCTTATGCTGCAAGCCAAATTACAAGAATTCAGGAAACAGTACCCACATGCAGTCATAGTTTATGCTGATTACTTCAATGCATACCGTACTGTGATGAAGAATCCAAGCAAGTATGGATTCAAAGATATGTTCAGTGTCTGCTGTGGATCAGGAGAACCTCCTTACAACTTTACTGTGTTTGCTACTTGTGGTACACCTAATGCTACTGTCTGCACTAGCCCTTCTCAGTATATTAATTGGGATGGTGTTCATCTTACCGAAGCTATGTATAAGGTTGTTTCTAATATGTTTCTTCAAGGAAATTTCAGCCAACCTCCATTTGATTTTTTGTTGGAAAACAAAGAGAGACATGGCTGA

Protein sequence

>MS.gene48401.t1

LLQLHQLQNNFQDLSRKFTPSETLSQTQATHTTLKVQVVLAMYQTHHMEQLSSTIPQTDILMEDLLLTLSQNHFLCLIFLLIVTSKDQMILLVLTLLLLVQQLUTMNSLLGIIFLLTSLLSLFKLRYCGLISTWRAKVVKELIQSVKILMRLFFGLVKLVLMIMLTLLDLQSQKILUGSLQCPVSQELYSHCLRRVPSTULCKVIHQQGALHUQCIULLQMTETTUDVUKVLTTUATITTLCCKPNYKNSGNSTHMQSUFMLITSMHTVLUURIQASMDSKICSVSAVDQENLLTTLLCLLLVVHLMLLSALALLSILIGMVFILPKLCIRLFLICFFKEISANLHLIFCWKTKRDMA