Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52353.t1 | XP_013460856.1 | 94.2 | 326 | 18 | 1 | 1 | 326 | 1 | 325 | 4.90E-170 | 607.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52353.t1 | Q55CU8 | 25.1 | 247 | 177 | 4 | 20 | 258 | 43 | 289 | 5.6e-21 | 103.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52353.t1 | G8A376 | 94.2 | 326 | 18 | 1 | 1 | 326 | 1 | 325 | 3.6e-170 | 607.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049134 | MS.gene52353 | -0.814914 | 1.21E-51 | -1.69E-46 |
| MS.gene049150 | MS.gene52353 | 0.825745 | 3.96E-54 | -1.69E-46 |
| MS.gene049153 | MS.gene52353 | 0.832548 | 8.88E-56 | -1.69E-46 |
| MS.gene049260 | MS.gene52353 | 0.805979 | 1.03E-49 | -1.69E-46 |
| MS.gene049356 | MS.gene52353 | 0.837924 | 3.90E-57 | -1.69E-46 |
| MS.gene049369 | MS.gene52353 | 0.819592 | 1.07E-52 | -1.69E-46 |
| MS.gene049758 | MS.gene52353 | 0.840482 | 8.47E-58 | -1.69E-46 |
| MS.gene050128 | MS.gene52353 | 0.81798 | 2.49E-52 | -1.69E-46 |
| MS.gene050301 | MS.gene52353 | 0.84629 | 2.38E-59 | -1.69E-46 |
| MS.gene050397 | MS.gene52353 | 0.815332 | 9.76E-52 | -1.69E-46 |
| MS.gene050432 | MS.gene52353 | 0.824356 | 8.44E-54 | -1.69E-46 |
| MS.gene050464 | MS.gene52353 | 0.801261 | 9.79E-49 | -1.69E-46 |
| MS.gene050670 | MS.gene52353 | 0.835265 | 1.86E-56 | -1.69E-46 |
| MS.gene05105 | MS.gene52353 | 0.807811 | 4.21E-50 | -1.69E-46 |
| MS.gene051360 | MS.gene52353 | 0.830648 | 2.61E-55 | -1.69E-46 |
| MS.gene051525 | MS.gene52353 | 0.803098 | 4.10E-49 | -1.69E-46 |
| MS.gene051582 | MS.gene52353 | 0.824688 | 7.05E-54 | -1.69E-46 |
| MS.gene051860 | MS.gene52353 | 0.834961 | 2.21E-56 | -1.69E-46 |
| MS.gene052011 | MS.gene52353 | -0.802602 | 5.19E-49 | -1.69E-46 |
| MS.gene052025 | MS.gene52353 | 0.8021 | 6.58E-49 | -1.69E-46 |
| MS.gene052077 | MS.gene52353 | 0.816008 | 6.90E-52 | -1.69E-46 |
| MS.gene052178 | MS.gene52353 | 0.818161 | 2.26E-52 | -1.69E-46 |
| MS.gene052188 | MS.gene52353 | 0.844574 | 6.95E-59 | -1.69E-46 |
| MS.gene052253 | MS.gene52353 | 0.825104 | 5.62E-54 | -1.69E-46 |
| MS.gene052310 | MS.gene52353 | 0.803726 | 3.04E-49 | -1.69E-46 |
| MS.gene052340 | MS.gene52353 | 0.854237 | 1.41E-61 | -1.69E-46 |
| MS.gene052485 | MS.gene52353 | 0.832504 | 9.10E-56 | -1.69E-46 |
| MS.gene052508 | MS.gene52353 | 0.805547 | 1.27E-49 | -1.69E-46 |
| MS.gene053073 | MS.gene52353 | 0.803409 | 3.53E-49 | -1.69E-46 |
| MS.gene05316 | MS.gene52353 | 0.82655 | 2.55E-54 | -1.69E-46 |
| MS.gene053209 | MS.gene52353 | 0.843993 | 9.96E-59 | -1.69E-46 |
| MS.gene053449 | MS.gene52353 | 0.824855 | 6.44E-54 | -1.69E-46 |
| MS.gene053557 | MS.gene52353 | 0.816991 | 4.16E-52 | -1.69E-46 |
| MS.gene053813 | MS.gene52353 | 0.800024 | 1.75E-48 | -1.69E-46 |
| MS.gene053836 | MS.gene52353 | 0.821878 | 3.19E-53 | -1.69E-46 |
| MS.gene054010 | MS.gene52353 | 0.818303 | 2.10E-52 | -1.69E-46 |
| MS.gene054011 | MS.gene52353 | 0.801107 | 1.05E-48 | -1.69E-46 |
| MS.gene054012 | MS.gene52353 | 0.802938 | 4.42E-49 | -1.69E-46 |
| MS.gene054183 | MS.gene52353 | 0.821014 | 5.06E-53 | -1.69E-46 |
| MS.gene054473 | MS.gene52353 | 0.809375 | 1.95E-50 | -1.69E-46 |
| MS.gene054556 | MS.gene52353 | 0.809084 | 2.25E-50 | -1.69E-46 |
| MS.gene054918 | MS.gene52353 | 0.805695 | 1.18E-49 | -1.69E-46 |
| MS.gene055116 | MS.gene52353 | 0.85712 | 2.03E-62 | -1.69E-46 |
| MS.gene055277 | MS.gene52353 | 0.831728 | 1.42E-55 | -1.69E-46 |
| MS.gene055390 | MS.gene52353 | 0.802034 | 6.79E-49 | -1.69E-46 |
| MS.gene055422 | MS.gene52353 | 0.80892 | 2.44E-50 | -1.69E-46 |
| MS.gene055428 | MS.gene52353 | 0.804935 | 1.70E-49 | -1.69E-46 |
| MS.gene05553 | MS.gene52353 | 0.823258 | 1.53E-53 | -1.69E-46 |
| MS.gene055604 | MS.gene52353 | 0.820676 | 6.05E-53 | -1.69E-46 |
| MS.gene055666 | MS.gene52353 | 0.826963 | 2.03E-54 | -1.69E-46 |
| MS.gene055859 | MS.gene52353 | 0.83238 | 9.77E-56 | -1.69E-46 |
| MS.gene056191 | MS.gene52353 | 0.8108 | 9.63E-51 | -1.69E-46 |
| MS.gene056407 | MS.gene52353 | 0.849699 | 2.73E-60 | -1.69E-46 |
| MS.gene056410 | MS.gene52353 | 0.803839 | 2.88E-49 | -1.69E-46 |
| MS.gene056422 | MS.gene52353 | 0.826988 | 2.00E-54 | -1.69E-46 |
| MS.gene056511 | MS.gene52353 | 0.844578 | 6.93E-59 | -1.69E-46 |
| MS.gene056899 | MS.gene52353 | -0.801328 | 9.48E-49 | -1.69E-46 |
| MS.gene056902 | MS.gene52353 | 0.814063 | 1.86E-51 | -1.69E-46 |
| MS.gene05702 | MS.gene52353 | 0.831747 | 1.40E-55 | -1.69E-46 |
| MS.gene057124 | MS.gene52353 | 0.814787 | 1.29E-51 | -1.69E-46 |
| MS.gene057396 | MS.gene52353 | 0.813323 | 2.71E-51 | -1.69E-46 |
| MS.gene057448 | MS.gene52353 | 0.866817 | 2.16E-65 | -1.69E-46 |
| MS.gene057449 | MS.gene52353 | 0.865555 | 5.43E-65 | -1.69E-46 |
| MS.gene057450 | MS.gene52353 | 0.870321 | 1.59E-66 | -1.69E-46 |
| MS.gene057469 | MS.gene52353 | 0.820143 | 8.01E-53 | -1.69E-46 |
| MS.gene057537 | MS.gene52353 | 0.808889 | 2.48E-50 | -1.69E-46 |
| MS.gene057734 | MS.gene52353 | 0.805642 | 1.21E-49 | -1.69E-46 |
| MS.gene057749 | MS.gene52353 | 0.814583 | 1.43E-51 | -1.69E-46 |
| MS.gene057823 | MS.gene52353 | 0.812591 | 3.93E-51 | -1.69E-46 |
| MS.gene057927 | MS.gene52353 | 0.810152 | 1.33E-50 | -1.69E-46 |
| MS.gene058002 | MS.gene52353 | 0.829008 | 6.54E-55 | -1.69E-46 |
| MS.gene05811 | MS.gene52353 | 0.844469 | 7.42E-59 | -1.69E-46 |
| MS.gene05842 | MS.gene52353 | 0.847062 | 1.47E-59 | -1.69E-46 |
| MS.gene058459 | MS.gene52353 | 0.808955 | 2.40E-50 | -1.69E-46 |
| MS.gene058700 | MS.gene52353 | 0.815775 | 7.78E-52 | -1.69E-46 |
| MS.gene05886 | MS.gene52353 | 0.801943 | 7.09E-49 | -1.69E-46 |
| MS.gene058928 | MS.gene52353 | 0.811145 | 8.10E-51 | -1.69E-46 |
| MS.gene058949 | MS.gene52353 | 0.80688 | 6.64E-50 | -1.69E-46 |
| MS.gene05907 | MS.gene52353 | 0.850002 | 2.25E-60 | -1.69E-46 |
| MS.gene059285 | MS.gene52353 | 0.804727 | 1.88E-49 | -1.69E-46 |
| MS.gene059294 | MS.gene52353 | 0.827412 | 1.59E-54 | -1.69E-46 |
| MS.gene059366 | MS.gene52353 | 0.825445 | 4.67E-54 | -1.69E-46 |
| MS.gene059420 | MS.gene52353 | 0.856235 | 3.69E-62 | -1.69E-46 |
| MS.gene059597 | MS.gene52353 | 0.809289 | 2.04E-50 | -1.69E-46 |
| MS.gene059676 | MS.gene52353 | 0.824647 | 7.21E-54 | -1.69E-46 |
| MS.gene059688 | MS.gene52353 | 0.846133 | 2.63E-59 | -1.69E-46 |
| MS.gene059692 | MS.gene52353 | 0.831981 | 1.23E-55 | -1.69E-46 |
| MS.gene059695 | MS.gene52353 | 0.832809 | 7.65E-56 | -1.69E-46 |
| MS.gene059711 | MS.gene52353 | 0.832832 | 7.55E-56 | -1.69E-46 |
| MS.gene059807 | MS.gene52353 | 0.859384 | 4.29E-63 | -1.69E-46 |
| MS.gene05984 | MS.gene52353 | 0.8173 | 3.54E-52 | -1.69E-46 |
| MS.gene059876 | MS.gene52353 | 0.806232 | 9.09E-50 | -1.69E-46 |
| MS.gene059894 | MS.gene52353 | 0.814884 | 1.23E-51 | -1.69E-46 |
| MS.gene059958 | MS.gene52353 | 0.820964 | 5.19E-53 | -1.69E-46 |
| MS.gene06037 | MS.gene52353 | 0.830221 | 3.32E-55 | -1.69E-46 |
| MS.gene060403 | MS.gene52353 | 0.84872 | 5.12E-60 | -1.69E-46 |
| MS.gene060495 | MS.gene52353 | 0.83319 | 6.15E-56 | -1.69E-46 |
| MS.gene060837 | MS.gene52353 | 0.855839 | 4.82E-62 | -1.69E-46 |
| MS.gene060927 | MS.gene52353 | 0.800506 | 1.40E-48 | -1.69E-46 |
| MS.gene060986 | MS.gene52353 | 0.803552 | 3.30E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene52353 | 0.850344 | 1.80E-60 | -1.69E-46 |
| MS.gene061051 | MS.gene52353 | -0.83505 | 2.10E-56 | -1.69E-46 |
| MS.gene061154 | MS.gene52353 | 0.843929 | 1.04E-58 | -1.69E-46 |
| MS.gene061159 | MS.gene52353 | 0.819281 | 1.26E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52353.t1 | MTR_3g074050 | 94.172 | 326 | 18 | 1 | 1 | 326 | 1 | 325 | 0.0 | 638 |
| MS.gene52353.t1 | MTR_3g074050 | 93.865 | 326 | 18 | 2 | 1 | 326 | 1 | 324 | 0.0 | 632 |
| MS.gene52353.t1 | MTR_3g074050 | 93.411 | 258 | 16 | 1 | 69 | 326 | 1 | 257 | 0.0 | 508 |
| MS.gene52353.t1 | MTR_5g062310 | 64.821 | 307 | 105 | 1 | 1 | 307 | 1 | 304 | 1.53e-147 | 419 |
| MS.gene52353.t1 | MTR_5g062310 | 64.435 | 239 | 85 | 0 | 69 | 307 | 1 | 239 | 3.69e-115 | 334 |
| MS.gene52353.t1 | MTR_7g087500 | 44.128 | 281 | 153 | 1 | 23 | 303 | 18 | 294 | 1.66e-90 | 273 |
| MS.gene52353.t1 | MTR_8g077700 | 47.638 | 254 | 132 | 1 | 18 | 270 | 12 | 265 | 4.17e-84 | 256 |
| MS.gene52353.t1 | MTR_8g077700 | 47.638 | 254 | 132 | 1 | 18 | 270 | 40 | 293 | 3.16e-83 | 254 |
| MS.gene52353.t1 | MTR_1g022185 | 44.309 | 246 | 136 | 1 | 26 | 270 | 20 | 265 | 5.86e-77 | 238 |
| MS.gene52353.t1 | MTR_4g105450 | 27.811 | 169 | 119 | 2 | 52 | 218 | 72 | 239 | 1.13e-22 | 95.5 |
| MS.gene52353.t1 | MTR_8g096180 | 29.386 | 228 | 154 | 7 | 20 | 244 | 19 | 242 | 2.86e-22 | 94.4 |
| MS.gene52353.t1 | MTR_4g105460 | 28.662 | 157 | 110 | 1 | 55 | 209 | 77 | 233 | 3.37e-21 | 91.7 |
| MS.gene52353.t1 | MTR_8g096170 | 28.634 | 227 | 155 | 7 | 21 | 244 | 23 | 245 | 2.42e-19 | 86.3 |
| MS.gene52353.t1 | MTR_8g096180 | 28.151 | 238 | 154 | 8 | 20 | 244 | 19 | 252 | 4.93e-19 | 85.5 |
| MS.gene52353.t1 | MTR_3g098530 | 26.106 | 226 | 160 | 7 | 22 | 244 | 41 | 262 | 9.52e-19 | 84.7 |
| MS.gene52353.t1 | MTR_1g104540 | 27.861 | 201 | 138 | 3 | 21 | 215 | 8 | 207 | 1.11e-17 | 83.6 |
| MS.gene52353.t1 | MTR_4g105460 | 28.358 | 134 | 90 | 3 | 55 | 185 | 77 | 207 | 8.84e-13 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52353.t1 | AT4G08690 | 51.751 | 257 | 121 | 1 | 9 | 265 | 6 | 259 | 1.58e-102 | 303 |
| MS.gene52353.t1 | AT4G08690 | 51.751 | 257 | 121 | 1 | 9 | 265 | 6 | 259 | 1.58e-102 | 303 |
| MS.gene52353.t1 | AT4G08690 | 51.751 | 257 | 121 | 1 | 9 | 265 | 6 | 259 | 1.58e-102 | 303 |
| MS.gene52353.t1 | AT1G22180 | 50.000 | 306 | 138 | 5 | 12 | 311 | 12 | 308 | 2.39e-101 | 300 |
| MS.gene52353.t1 | AT1G22180 | 50.000 | 306 | 138 | 5 | 12 | 311 | 12 | 308 | 2.39e-101 | 300 |
| MS.gene52353.t1 | AT1G22180 | 50.602 | 249 | 111 | 4 | 69 | 311 | 1 | 243 | 3.79e-84 | 254 |
| MS.gene52353.t1 | AT1G22180 | 50.602 | 249 | 111 | 4 | 69 | 311 | 1 | 243 | 3.79e-84 | 254 |
| MS.gene52353.t1 | AT1G75170 | 43.023 | 258 | 146 | 1 | 25 | 281 | 20 | 277 | 6.37e-79 | 243 |
| MS.gene52353.t1 | AT1G75170 | 43.023 | 258 | 146 | 1 | 25 | 281 | 20 | 277 | 6.37e-79 | 243 |
| MS.gene52353.t1 | AT4G36640 | 41.199 | 267 | 156 | 1 | 24 | 289 | 16 | 282 | 4.33e-75 | 233 |
| MS.gene52353.t1 | AT4G36640 | 41.199 | 267 | 156 | 1 | 24 | 289 | 16 | 282 | 4.33e-75 | 233 |
| MS.gene52353.t1 | AT4G36640 | 40.991 | 222 | 130 | 1 | 69 | 289 | 1 | 222 | 1.11e-59 | 191 |
| MS.gene52353.t1 | AT1G75170 | 39.896 | 193 | 115 | 1 | 90 | 281 | 2 | 194 | 7.62e-49 | 163 |
| MS.gene52353.t1 | AT5G63060 | 30.769 | 156 | 106 | 1 | 55 | 208 | 78 | 233 | 2.82e-21 | 91.7 |
| MS.gene52353.t1 | AT5G63060 | 30.818 | 159 | 108 | 1 | 52 | 208 | 40 | 198 | 3.37e-21 | 90.9 |
| MS.gene52353.t1 | AT1G01630 | 27.347 | 245 | 171 | 7 | 3 | 244 | 5 | 245 | 4.10e-19 | 85.5 |
| MS.gene52353.t1 | AT1G05370 | 26.257 | 179 | 129 | 2 | 39 | 215 | 32 | 209 | 6.17e-14 | 72.4 |
| MS.gene52353.t1 | AT3G22410 | 26.728 | 217 | 143 | 6 | 24 | 225 | 6 | 221 | 1.39e-12 | 68.6 |
Find 67 sgRNAs with CRISPR-Local
Find 94 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATATTCCTGTCTCCATTTA+AGG | 0.158580 | 3.1:+60599354 | None:intergenic |
| CCATAATCTTCTTTGTGTTT+TGG | 0.230567 | 3.1:+60598460 | None:intergenic |
| GGTATTCAAAATCACAAAAT+TGG | 0.231715 | 3.1:-60599412 | MS.gene52353:CDS |
| CCATCCTTGGCACAATAATT+TGG | 0.234391 | 3.1:+60599100 | None:intergenic |
| TATGGAGGGTTTGTTTGATA+TGG | 0.249989 | 3.1:-60598442 | MS.gene52353:CDS |
| ATAACAAGATGCACTCCTTT+TGG | 0.282527 | 3.1:-60598338 | MS.gene52353:CDS |
| CTTTATCTTATTATAATTCT+CGG | 0.306203 | 3.1:+60598500 | None:intergenic |
| CTTACCCAGGTAATCTCTTC+AGG | 0.306944 | 3.1:+60599329 | None:intergenic |
| CCAAAACACAAAGAAGATTA+TGG | 0.309315 | 3.1:-60598460 | MS.gene52353:CDS |
| GAGATATATTGATCAGGTTT+AGG | 0.320441 | 3.1:+60598201 | None:intergenic |
| CTCACCAGGAGCAGGTGATC+TGG | 0.403831 | 3.1:-60598857 | MS.gene52353:CDS |
| TTTGTTCTCATTATAAGTAC+TGG | 0.408192 | 3.1:+60599073 | None:intergenic |
| GTTCTGATGCATCCATTTCA+AGG | 0.412879 | 3.1:-60599433 | MS.gene52353:CDS |
| ATCTGGCTGGTTGATTTCCA+TGG | 0.440350 | 3.1:-60598840 | MS.gene52353:CDS |
| GATCAACTTGAACCTGCATT+TGG | 0.447521 | 3.1:-60598420 | MS.gene52353:CDS |
| AAGTACTGGTCTTCCATCCT+TGG | 0.473632 | 3.1:+60599087 | None:intergenic |
| GTTGCTAATGAAGCAGAGAC+AGG | 0.477872 | 3.1:-60599136 | MS.gene52353:CDS |
| TATTTCGTTACCGAGAAGAA+TGG | 0.481590 | 3.1:+60598699 | None:intergenic |
| ATTGAGGTGAGAAAGTTGAT+AGG | 0.483915 | 3.1:-60599485 | MS.gene52353:CDS |
| TTTAAACCTTCCACCTCACC+AGG | 0.488686 | 3.1:-60598871 | MS.gene52353:CDS |
| GTAACTGAGATATATTGATC+AGG | 0.500083 | 3.1:+60598195 | None:intergenic |
| CCAGGAGCAGGTGATCTGGC+TGG | 0.500834 | 3.1:-60598853 | MS.gene52353:CDS |
| CCAAATTATTGTGCCAAGGA+TGG | 0.506258 | 3.1:-60599100 | MS.gene52353:CDS |
| TCATTATTCCCACCAAATGC+AGG | 0.506468 | 3.1:+60598408 | None:intergenic |
| CCTGCTCCTGGTGAGGTGGA+AGG | 0.508219 | 3.1:+60598865 | None:intergenic |
| CAACTTGAACCTGCATTTGG+TGG | 0.510649 | 3.1:-60598417 | MS.gene52353:CDS |
| GTCACTATCACAAGAACAGC+AGG | 0.515426 | 3.1:-60599619 | MS.gene52353:CDS |
| AGAGTTTGCATGTGTCCAAA+AGG | 0.518082 | 3.1:+60598323 | None:intergenic |
| ATCACCTGCTCCTGGTGAGG+TGG | 0.523818 | 3.1:+60598861 | None:intergenic |
| CTGAAGAAAACTGCCTCTGA+TGG | 0.531528 | 3.1:-60599663 | MS.gene52353:CDS |
| TTCCTCCTTCAGATTTGACC+AGG | 0.531700 | 3.1:-60598278 | MS.gene52353:CDS |
| CACTTGACAAACTGAAACCA+TGG | 0.532083 | 3.1:+60598823 | None:intergenic |
| CCAGCCAGATCACCTGCTCC+TGG | 0.532714 | 3.1:+60598853 | None:intergenic |
| ATAAACCTGAAGAGATTACC+TGG | 0.535260 | 3.1:-60599334 | MS.gene52353:CDS |
| GAAGAATGGTTGGAAAATCC+CGG | 0.535429 | 3.1:+60598713 | None:intergenic |
| TTATCCACAGCGCCTCGGAT+TGG | 0.536069 | 3.1:-60598754 | MS.gene52353:CDS |
| GATAGGAACATTATCAGATA+AGG | 0.538137 | 3.1:-60599468 | MS.gene52353:CDS |
| CAGATCACCTGCTCCTGGTG+AGG | 0.548938 | 3.1:+60598858 | None:intergenic |
| AACTTGAACCTGCATTTGGT+GGG | 0.550189 | 3.1:-60598416 | MS.gene52353:CDS |
| TATAATGAGAACAAATCGCC+AGG | 0.555150 | 3.1:-60599065 | MS.gene52353:intron |
| CCTTCCACCTCACCAGGAGC+AGG | 0.557345 | 3.1:-60598865 | MS.gene52353:CDS |
| AATATATCTCAGTTACTCGC+GGG | 0.560385 | 3.1:-60598188 | MS.gene52353:CDS |
| ATCAGAATCTGCTACTAACC+TGG | 0.564891 | 3.1:+60598260 | None:intergenic |
| GCAATCATGTATGATGCACC+CGG | 0.576986 | 3.1:-60598732 | MS.gene52353:CDS |
| AAATATTATCCACAGCGCCT+CGG | 0.585906 | 3.1:-60598759 | MS.gene52353:CDS |
| TGGTGCATTCCAGATCATTG+AGG | 0.589779 | 3.1:-60599501 | MS.gene52353:intron |
| TCGTTACCGAGAAGAATGGT+TGG | 0.590066 | 3.1:+60598703 | None:intergenic |
| AACACAAAGAAGATTATGGA+GGG | 0.591841 | 3.1:-60598456 | MS.gene52353:CDS |
| ATCACAAGAACAGCAGGCTA+AGG | 0.594560 | 3.1:-60599613 | MS.gene52353:intron |
| ACATTGTGTGAGACTGATGA+TGG | 0.595579 | 3.1:+60598300 | None:intergenic |
| AAGAATGGTTGGAAAATCCC+GGG | 0.597907 | 3.1:+60598714 | None:intergenic |
| ATATGCTAAGAAAATGAGAG+AGG | 0.599794 | 3.1:-60598364 | MS.gene52353:CDS |
| ACTAACCTGGTCAAATCTGA+AGG | 0.603314 | 3.1:+60598273 | None:intergenic |
| TGAATTGTTGCAGATGGTGA+AGG | 0.607257 | 3.1:-60598535 | MS.gene52353:intron |
| TAGACCAAATTATTGTGCCA+AGG | 0.612792 | 3.1:-60599104 | MS.gene52353:CDS |
| AAACACAAAGAAGATTATGG+AGG | 0.618726 | 3.1:-60598457 | MS.gene52353:CDS |
| TCGCGGGGACACAAAGGTCA+TGG | 0.619216 | 3.1:-60598172 | MS.gene52353:CDS |
| CAATATATCTCAGTTACTCG+CGG | 0.625172 | 3.1:-60598189 | MS.gene52353:CDS |
| TGTTCTTGTGATAGTGACAC+TGG | 0.627385 | 3.1:+60599624 | None:intergenic |
| ATTGCCAATCCGAGGCGCTG+TGG | 0.630157 | 3.1:+60598750 | None:intergenic |
| AGTTACTCGCGGGGACACAA+AGG | 0.631514 | 3.1:-60598178 | MS.gene52353:CDS |
| CATACATGATTGCCAATCCG+AGG | 0.632178 | 3.1:+60598742 | None:intergenic |
| AACCTGGTCAAATCTGAAGG+AGG | 0.655670 | 3.1:+60598276 | None:intergenic |
| TTGCTAATGAAGCAGAGACA+GGG | 0.659274 | 3.1:-60599135 | MS.gene52353:CDS |
| CAATCATGTATGATGCACCC+GGG | 0.666450 | 3.1:-60598731 | MS.gene52353:CDS |
| TAAACCTGAAGAGATTACCT+GGG | 0.681766 | 3.1:-60599333 | MS.gene52353:intron |
| ATATATCTCAGTTACTCGCG+GGG | 0.688008 | 3.1:-60598187 | MS.gene52353:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTAAATATCAACATTTTA+CGG | + | chr3.1:60599225-60599244 | None:intergenic | 10.0% |
| !!! | GATAATATTTTTGTAATATA+TGG | + | chr3.1:60599073-60599092 | None:intergenic | 10.0% |
| !! | CTTTATCTTATTATAATTCT+CGG | + | chr3.1:60599345-60599364 | None:intergenic | 15.0% |
| !! | TAGCTGATAATAGTTTAATT+TGG | - | chr3.1:60598321-60598340 | MS.gene52353:CDS | 20.0% |
| !!! | TTAACAAGTATGTTTTTTCT+TGG | - | chr3.1:60598672-60598691 | MS.gene52353:intron | 20.0% |
| ! | AACAAAGCCTAAAATTGTTA+TGG | - | chr3.1:60598578-60598597 | MS.gene52353:intron | 25.0% |
| ! | AGAACATTGATCAAACTTAT+CGG | + | chr3.1:60599262-60599281 | None:intergenic | 25.0% |
| ! | ATGTCCAATAATTGAAATCT+TGG | - | chr3.1:60598253-60598272 | MS.gene52353:CDS | 25.0% |
| ! | GGTATTCAAAATCACAAAAT+TGG | - | chr3.1:60598430-60598449 | MS.gene52353:CDS | 25.0% |
| ! | TGTTGAAAAAAAGCCTTAAA+TGG | - | chr3.1:60598475-60598494 | MS.gene52353:CDS | 25.0% |
| ! | TTTGTTCTCATTATAAGTAC+TGG | + | chr3.1:60598772-60598791 | None:intergenic | 25.0% |
| !! | TGATTTTGAATACCTTGAAA+TGG | + | chr3.1:60598424-60598443 | None:intergenic | 25.0% |
| !!! | AACAACAATATTTTGAGTGA+AGG | - | chr3.1:60598292-60598311 | MS.gene52353:CDS | 25.0% |
| !!! | AGTATGTTTTTTCTTGGTTA+AGG | - | chr3.1:60598678-60598697 | MS.gene52353:intron | 25.0% |
| !!! | TTTTTTTTTACAGCAGAAGT+TGG | - | chr3.1:60599197-60599216 | MS.gene52353:intron | 25.0% |
| AAACACAAAGAAGATTATGG+AGG | - | chr3.1:60599385-60599404 | MS.gene52353:CDS | 30.0% | |
| AAAGAATGAATTGTTGCAGA+TGG | - | chr3.1:60599301-60599320 | MS.gene52353:intron | 30.0% | |
| AACACAAAGAAGATTATGGA+GGG | - | chr3.1:60599386-60599405 | MS.gene52353:CDS | 30.0% | |
| ATATGCTAAGAAAATGAGAG+AGG | - | chr3.1:60599478-60599497 | MS.gene52353:CDS | 30.0% | |
| CCAAAACACAAAGAAGATTA+TGG | - | chr3.1:60599382-60599401 | MS.gene52353:CDS | 30.0% | |
| GAGATATATTGATCAGGTTT+AGG | + | chr3.1:60599644-60599663 | None:intergenic | 30.0% | |
| GATAGGAACATTATCAGATA+AGG | - | chr3.1:60598374-60598393 | MS.gene52353:CDS | 30.0% | |
| GTAACTGAGATATATTGATC+AGG | + | chr3.1:60599650-60599669 | None:intergenic | 30.0% | |
| TATATTCCTGTCTCCATTTA+AGG | + | chr3.1:60598491-60598510 | None:intergenic | 30.0% | |
| TCAGATTGAATTGAAAGATG+AGG | - | chr3.1:60598618-60598637 | MS.gene52353:intron | 30.0% | |
| TGCTCCAAGATTTCAATTAT+TGG | + | chr3.1:60598260-60598279 | None:intergenic | 30.0% | |
| ! | AACACAGCCATAACAATTTT+AGG | + | chr3.1:60598588-60598607 | None:intergenic | 30.0% |
| ! | CCATAATCTTCTTTGTGTTT+TGG | + | chr3.1:60599385-60599404 | None:intergenic | 30.0% |
| !! | AATATTTTGAGTGAAGGATG+AGG | - | chr3.1:60598298-60598317 | MS.gene52353:CDS | 30.0% |
| !! | TAAGCATTTTGTTTACTGCA+TGG | - | chr3.1:60598938-60598957 | MS.gene52353:intron | 30.0% |
| AAAAAGCCTTAAATGGAGAC+AGG | - | chr3.1:60598482-60598501 | MS.gene52353:CDS | 35.0% | |
| AATATATCTCAGTTACTCGC+GGG | - | chr3.1:60599654-60599673 | MS.gene52353:CDS | 35.0% | |
| ACTTCAGTACGAGTGAAAAA+TGG | - | chr3.1:60598839-60598858 | MS.gene52353:CDS | 35.0% | |
| ATAAACCTGAAGAGATTACC+TGG | - | chr3.1:60598508-60598527 | MS.gene52353:CDS | 35.0% | |
| CAATATATCTCAGTTACTCG+CGG | - | chr3.1:60599653-60599672 | MS.gene52353:CDS | 35.0% | |
| TAAACCTGAAGAGATTACCT+GGG | - | chr3.1:60598509-60598528 | MS.gene52353:CDS | 35.0% | |
| TAGACCAAATTATTGTGCCA+AGG | - | chr3.1:60598738-60598757 | MS.gene52353:CDS | 35.0% | |
| TATAATGAGAACAAATCGCC+AGG | - | chr3.1:60598777-60598796 | MS.gene52353:CDS | 35.0% | |
| TATTTCGTTACCGAGAAGAA+TGG | + | chr3.1:60599146-60599165 | None:intergenic | 35.0% | |
| ! | ACACTGGTAACATTTTCTCA+TGG | + | chr3.1:60598205-60598224 | None:intergenic | 35.0% |
| ! | AGTGATTTTCAGAAGAAACC+TGG | + | chr3.1:60598798-60598817 | None:intergenic | 35.0% |
| ! | ATAACAAGATGCACTCCTTT+TGG | - | chr3.1:60599504-60599523 | MS.gene52353:intron | 35.0% |
| ! | ATCAACTATTTTCTCGTCGT+TGG | + | chr3.1:60599612-60599631 | None:intergenic | 35.0% |
| ! | ATTGAGGTGAGAAAGTTGAT+AGG | - | chr3.1:60598357-60598376 | MS.gene52353:CDS | 35.0% |
| ! | GATTTTCCAACCATTCTTCT+CGG | - | chr3.1:60599133-60599152 | MS.gene52353:CDS | 35.0% |
| ! | TATGGAGGGTTTGTTTGATA+TGG | - | chr3.1:60599400-60599419 | MS.gene52353:CDS | 35.0% |
| AAATATTATCCACAGCGCCT+CGG | - | chr3.1:60599083-60599102 | MS.gene52353:CDS | 40.0% | |
| AACTTGAACCTGCATTTGGT+GGG | - | chr3.1:60599426-60599445 | MS.gene52353:CDS | 40.0% | |
| AAGAATGGTTGGAAAATCCC+GGG | + | chr3.1:60599131-60599150 | None:intergenic | 40.0% | |
| ACATTGTGTGAGACTGATGA+TGG | + | chr3.1:60599545-60599564 | None:intergenic | 40.0% | |
| ACTAACCTGGTCAAATCTGA+AGG | + | chr3.1:60599572-60599591 | None:intergenic | 40.0% | |
| ACTTTCTCACCTCAATGATC+TGG | + | chr3.1:60598353-60598372 | None:intergenic | 40.0% | |
| AGAGTTTGCATGTGTCCAAA+AGG | + | chr3.1:60599522-60599541 | None:intergenic | 40.0% | |
| ATATATCTCAGTTACTCGCG+GGG | - | chr3.1:60599655-60599674 | MS.gene52353:CDS | 40.0% | |
| ATCAGAATCTGCTACTAACC+TGG | + | chr3.1:60599585-60599604 | None:intergenic | 40.0% | |
| CACTTGACAAACTGAAACCA+TGG | + | chr3.1:60599022-60599041 | None:intergenic | 40.0% | |
| CCAAATTATTGTGCCAAGGA+TGG | - | chr3.1:60598742-60598761 | MS.gene52353:CDS | 40.0% | |
| CCATCCTTGGCACAATAATT+TGG | + | chr3.1:60598745-60598764 | None:intergenic | 40.0% | |
| GAAGAATGGTTGGAAAATCC+CGG | + | chr3.1:60599132-60599151 | None:intergenic | 40.0% | |
| GATCAACTTGAACCTGCATT+TGG | - | chr3.1:60599422-60599441 | MS.gene52353:CDS | 40.0% | |
| GTTCTGATGCATCCATTTCA+AGG | - | chr3.1:60598409-60598428 | MS.gene52353:CDS | 40.0% | |
| TCATTATTCCCACCAAATGC+AGG | + | chr3.1:60599437-60599456 | None:intergenic | 40.0% | |
| TGAATTGTTGCAGATGGTGA+AGG | - | chr3.1:60599307-60599326 | MS.gene52353:intron | 40.0% | |
| TTGCTAATGAAGCAGAGACA+GGG | - | chr3.1:60598707-60598726 | MS.gene52353:intron | 40.0% | |
| ! | TGTTCTTGTGATAGTGACAC+TGG | + | chr3.1:60598221-60598240 | None:intergenic | 40.0% |
| AACCTGGTCAAATCTGAAGG+AGG | + | chr3.1:60599569-60599588 | None:intergenic | 45.0% | |
| AAGCAAGCAGTAACTTACCC+AGG | + | chr3.1:60598529-60598548 | None:intergenic | 45.0% | |
| ATCACAAGAACAGCAGGCTA+AGG | - | chr3.1:60598229-60598248 | MS.gene52353:CDS | 45.0% | |
| CAACTTGAACCTGCATTTGG+TGG | - | chr3.1:60599425-60599444 | MS.gene52353:CDS | 45.0% | |
| CAATCATGTATGATGCACCC+GGG | - | chr3.1:60599111-60599130 | MS.gene52353:CDS | 45.0% | |
| CATACATGATTGCCAATCCG+AGG | + | chr3.1:60599103-60599122 | None:intergenic | 45.0% | |
| CTGAAGAAAACTGCCTCTGA+TGG | - | chr3.1:60598179-60598198 | MS.gene52353:CDS | 45.0% | |
| CTTACCCAGGTAATCTCTTC+AGG | + | chr3.1:60598516-60598535 | None:intergenic | 45.0% | |
| GCAATCATGTATGATGCACC+CGG | - | chr3.1:60599110-60599129 | MS.gene52353:CDS | 45.0% | |
| GTCACTATCACAAGAACAGC+AGG | - | chr3.1:60598223-60598242 | MS.gene52353:CDS | 45.0% | |
| GTTGCTAATGAAGCAGAGAC+AGG | - | chr3.1:60598706-60598725 | MS.gene52353:intron | 45.0% | |
| TCGTTACCGAGAAGAATGGT+TGG | + | chr3.1:60599142-60599161 | None:intergenic | 45.0% | |
| TGGTGCATTCCAGATCATTG+AGG | - | chr3.1:60598341-60598360 | MS.gene52353:CDS | 45.0% | |
| TTCCTCCTTCAGATTTGACC+AGG | - | chr3.1:60599564-60599583 | MS.gene52353:intron | 45.0% | |
| TTTAAACCTTCCACCTCACC+AGG | - | chr3.1:60598971-60598990 | MS.gene52353:intron | 45.0% | |
| ! | ATCTGGCTGGTTGATTTCCA+TGG | - | chr3.1:60599002-60599021 | MS.gene52353:intron | 45.0% |
| ! | CATTTTCTCATGGCCATCAG+AGG | + | chr3.1:60598195-60598214 | None:intergenic | 45.0% |
| !! | AAGTACTGGTCTTCCATCCT+TGG | + | chr3.1:60598758-60598777 | None:intergenic | 45.0% |
| AGTTACTCGCGGGGACACAA+AGG | - | chr3.1:60599664-60599683 | MS.gene52353:CDS | 55.0% | |
| TTATCCACAGCGCCTCGGAT+TGG | - | chr3.1:60599088-60599107 | MS.gene52353:CDS | 55.0% | |
| ATCACCTGCTCCTGGTGAGG+TGG | + | chr3.1:60598984-60599003 | None:intergenic | 60.0% | |
| CAGATCACCTGCTCCTGGTG+AGG | + | chr3.1:60598987-60599006 | None:intergenic | 60.0% | |
| CTCACCAGGAGCAGGTGATC+TGG | - | chr3.1:60598985-60599004 | MS.gene52353:intron | 60.0% | |
| TCGCGGGGACACAAAGGTCA+TGG | - | chr3.1:60599670-60599689 | MS.gene52353:CDS | 60.0% | |
| !! | ATTGCCAATCCGAGGCGCTG+TGG | + | chr3.1:60599095-60599114 | None:intergenic | 60.0% |
| CCAGCCAGATCACCTGCTCC+TGG | + | chr3.1:60598992-60599011 | None:intergenic | 65.0% | |
| CCAGGAGCAGGTGATCTGGC+TGG | - | chr3.1:60598989-60599008 | MS.gene52353:intron | 65.0% | |
| CCTGCTCCTGGTGAGGTGGA+AGG | + | chr3.1:60598980-60598999 | None:intergenic | 65.0% | |
| CCTTCCACCTCACCAGGAGC+AGG | - | chr3.1:60598977-60598996 | MS.gene52353:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 60598167 | 60599697 | 60598167 | ID=MS.gene52353 |
| chr3.1 | mRNA | 60598167 | 60599697 | 60598167 | ID=MS.gene52353.t1;Parent=MS.gene52353 |
| chr3.1 | exon | 60599614 | 60599697 | 60599614 | ID=MS.gene52353.t1.exon1;Parent=MS.gene52353.t1 |
| chr3.1 | CDS | 60599614 | 60599697 | 60599614 | ID=cds.MS.gene52353.t1;Parent=MS.gene52353.t1 |
| chr3.1 | exon | 60599334 | 60599510 | 60599334 | ID=MS.gene52353.t1.exon2;Parent=MS.gene52353.t1 |
| chr3.1 | CDS | 60599334 | 60599510 | 60599334 | ID=cds.MS.gene52353.t1;Parent=MS.gene52353.t1 |
| chr3.1 | exon | 60599066 | 60599164 | 60599066 | ID=MS.gene52353.t1.exon3;Parent=MS.gene52353.t1 |
| chr3.1 | CDS | 60599066 | 60599164 | 60599066 | ID=cds.MS.gene52353.t1;Parent=MS.gene52353.t1 |
| chr3.1 | exon | 60598710 | 60598952 | 60598710 | ID=MS.gene52353.t1.exon4;Parent=MS.gene52353.t1 |
| chr3.1 | CDS | 60598710 | 60598952 | 60598710 | ID=cds.MS.gene52353.t1;Parent=MS.gene52353.t1 |
| chr3.1 | exon | 60598167 | 60598544 | 60598167 | ID=MS.gene52353.t1.exon5;Parent=MS.gene52353.t1 |
| chr3.1 | CDS | 60598167 | 60598544 | 60598167 | ID=cds.MS.gene52353.t1;Parent=MS.gene52353.t1 |
| Gene Sequence |
| Protein sequence |
