AlfalfaGEDB | MS.gene58153

M. sativa cultivar XinJiangDaYe / MS.gene58153

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004553 GO:0016787 GO:0016798 GO:0033926 GO:0140096 GO:0140103
PFAM: PF12899.10

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58153.t1	XP_003630134.1	98.1	645	11	1	1	644	1	645	0.00E+00	1284.2

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58153.t1	Q6H6N5	76.5	562	113	4	84	644	63	606	1.8e-257	889.8

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58153.t1	G7LBD0	98.1	645	11	1	1	644	1	645	0.0e+00	1284.2

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049222	MS.gene58153	0.829226	5.79E-55	-1.69E-46
MS.gene049356	MS.gene58153	0.868057	8.66E-66	-1.69E-46
MS.gene049369	MS.gene58153	0.882615	8.98E-71	-1.69E-46
MS.gene049396	MS.gene58153	0.810164	1.32E-50	-1.69E-46
MS.gene049633	MS.gene58153	0.811068	8.42E-51	-1.69E-46
MS.gene049805	MS.gene58153	0.805166	1.52E-49	-1.69E-46
MS.gene050083	MS.gene58153	-0.811634	6.35E-51	-1.69E-46
MS.gene050444	MS.gene58153	0.81202	5.23E-51	-1.69E-46
MS.gene050553	MS.gene58153	0.822694	2.07E-53	-1.69E-46
MS.gene050747	MS.gene58153	0.834965	2.21E-56	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58153.t1	MTR_8g092170	98.140	645	11	1	1	644	1	645	0.0	1314
MS.gene58153.t1	MTR_1g084610	73.362	473	122	2	164	636	113	581	0.0	760
MS.gene58153.t1	MTR_4g123030	67.969	512	159	2	129	635	147	658	0.0	757
MS.gene58153.t1	MTR_7g111890	60.653	582	210	7	67	644	43	609	0.0	732
MS.gene58153.t1	MTR_4g123030	71.285	397	112	1	129	523	147	543	0.0	616
MS.gene58153.t1	MTR_1g096140	56.933	476	199	2	146	620	73	543	0.0	586
MS.gene58153.t1	MTR_3g117710	55.852	487	209	2	134	620	81	561	0.0	583
MS.gene58153.t1	MTR_1g095970	58.515	458	184	2	164	620	89	541	0.0	578
MS.gene58153.t1	MTR_4g075540	56.511	407	171	2	164	570	51	451	2.76e-171	496
MS.gene58153.t1	MTR_3g015100	69.935	153	46	0	371	523	1	153	2.04e-77	243

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
MS.gene58153.t1	AT5G22510	69.538	650	157	8	3	644	1	617	904
MS.gene58153.t1	AT5G22510	69.538	650	157	8	3	644	1	617	904
MS.gene58153.t1	AT1G56560	74.348	460	116	1	162	621	132	589	767
MS.gene58153.t1	AT3G06500	67.433	522	160	3	125	637	134	654	763
MS.gene58153.t1	AT3G05820	68.031	513	159	3	125	635	112	621	754
MS.gene58153.t1	AT3G06500	71.638	409	115	1	229	637	48	455	652
MS.gene58153.t1	AT3G05820	71.072	401	111	3	125	523	112	509	614
MS.gene58153.t1	AT4G09510	59.737	457	179	1	164	620	95	546	603
MS.gene58153.t1	AT1G35580	60.573	454	174	1	164	617	87	535	601
MS.gene58153.t1	AT1G35580	60.573	454	174	1	164	617	87	535	601

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Find 153 sgRNAs with CRISPR-Local

Find 355 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACATACTGATAACTGAAG+AGG	0.547667	4.2:+9978915	MS.gene58153:CDS
AACAAAGGAGGCTACTTAAT+TGG	0.324104	4.2:+9978051	MS.gene58153:CDS
AACAGGAGCAACACGGCCAA+TGG	0.563132	4.2:-9975687	None:intergenic
AACATGTGATCTCAGGGCTG+TGG	0.624246	4.2:+9975803	MS.gene58153:intron
AACCAAGGAGAAATCTGGTC+TGG	0.425338	4.2:-9978012	None:intergenic
AACCAGCTCACATGGATTTC+CGG	0.330359	4.2:+9978082	MS.gene58153:CDS
AACCGGAAATCCATGTGAGC+TGG	0.541172	4.2:-9978084	None:intergenic
AACTGGGAAGTGTTGACAAA+AGG	0.497494	4.2:-9974115	None:intergenic
AAGAAGCGTGGGATCTACTG+AGG	0.619813	4.2:+9974530	MS.gene58153:CDS
AATAGGAAAGCGACTCCAGA+TGG	0.548635	4.2:-9974661	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACATACTGATAACTGAAG+AGG	+	chr4.2:9978915-9978934	MS.gene58153:CDS	30.0%
AACATATACAATTTCAGAGC+TGG	+	chr4.2:9975534-9975553	MS.gene58153:intron	30.0%
AAGGTGAAATTACACAGATT+TGG	-	chr4.2:9976745-9976764	None:intergenic	30.0%
AATTATTGTGGGAATCCTAT+TGG	+	chr4.2:9974565-9974584	MS.gene58153:CDS	30.0%
AATTCAAACATGCAATGCTT+AGG	-	chr4.2:9978642-9978661	None:intergenic	30.0%
ACAGATTATGATAAGAGCTA+AGG	-	chr4.2:9978558-9978577	None:intergenic	30.0%
ACATATACAATTTCAGAGCT+GGG	+	chr4.2:9975535-9975554	MS.gene58153:intron	30.0%
AGGACAAGGTTTATTACTAA+AGG	+	chr4.2:9975358-9975377	MS.gene58153:intron	30.0%
AGGTGAAATTACACAGATTT+GGG	-	chr4.2:9976744-9976763	None:intergenic	30.0%
AGGTTTATTACTAAAGGAAG+GGG	+	chr4.2:9975364-9975383	MS.gene58153:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	9974043	9979023	9974043	ID=MS.gene58153
chr4.2	mRNA	9974043	9979023	9974043	ID=MS.gene58153.t1;Parent=MS.gene58153
chr4.2	exon	9974043	9974738	9974043	ID=MS.gene58153.t1.exon1;Parent=MS.gene58153.t1
chr4.2	CDS	9974043	9974738	9974043	ID=cds.MS.gene58153.t1;Parent=MS.gene58153.t1
chr4.2	exon	9975551	9975716	9975551	ID=MS.gene58153.t1.exon2;Parent=MS.gene58153.t1
chr4.2	CDS	9975551	9975716	9975551	ID=cds.MS.gene58153.t1;Parent=MS.gene58153.t1
chr4.2	exon	9975818	9976029	9975818	ID=MS.gene58153.t1.exon3;Parent=MS.gene58153.t1
chr4.2	CDS	9975818	9976029	9975818	ID=cds.MS.gene58153.t1;Parent=MS.gene58153.t1
chr4.2	exon	9977799	9978292	9977799	ID=MS.gene58153.t1.exon4;Parent=MS.gene58153.t1
chr4.2	CDS	9977799	9978292	9977799	ID=cds.MS.gene58153.t1;Parent=MS.gene58153.t1
chr4.2	exon	9978375	9978423	9978375	ID=MS.gene58153.t1.exon5;Parent=MS.gene58153.t1
chr4.2	CDS	9978375	9978423	9978375	ID=cds.MS.gene58153.t1;Parent=MS.gene58153.t1
chr4.2	exon	9978706	9979023	9978706	ID=MS.gene58153.t1.exon6;Parent=MS.gene58153.t1
chr4.2	CDS	9978706	9979023	9978706	ID=cds.MS.gene58153.t1;Parent=MS.gene58153.t1

Gene Sequence

>MS.gene58153

ATGTCTTTAGGTGCTTCCAAAGCCGTTTTTCAGGTTCTGTCCAGTGTTGTTCCTCAATCCGCGTATAATGAGCCTTTTGTCAACACTTCCCAGTTGCGAACAAATTGTATGAAGAAAAGATCCTCAAGGCATAGGTTTTTAATAGAGAGCTCTAGCATGCTTCAAAGCCAGTTAAGGCCCCATCGATTTCCATTTACCAGTGTCAGTTTCTGTGACTATAAGACCTATAGTCATCCATGGTTGCAGACATGTAAATGCCAAAAGGCTGAAAATGTGAGCGGCATAACTTCAGGAGACGGTAATGGATCAAGGTTTGCGAGTGATGTTGAAAAATCTAGTCTGGTAAGCAATGTGATGAGTGCAAAGTCTTCTTTAGAGTTTGAAGATGTTCAACTGTTGGAACAGGAAAAAGAGGTTTTGTCATCCAATGTTACAAATGGGACTGCCACGAAAAATCTTGGTACAATTAGCCTCAACTCTATTGAGGAAGAAGCGTGGGATCTACTGAGGGAGTCTGTAGTTAATTATTGTGGGAATCCTATTGGAACTATTGCTGCAAAGGATCCAAACAGTACCAATGTTTTGAATTATGACCAAGTCTTTATTCGTGATTTTATTCCATCTGGAGTCGCTTTCCTATTGAAGGGAGAGTATGATATTGTTCGTAATTTCATTCTTCATACACTTCAGCTGCAGGTAATCCCTTACTCTTATATTACTGATAGCATGTTCTTGGAACAGAACTAAATTTGATACAACTTCGTGCATTTACATGTCTGAGATGCTGCGTATGTTCATTTTTTAATTGATTGTGTTGAGCTGATGTGCGTATTCTTTGTGTTATGTCTTTACAAACTTATTCCATTCATTAGTCATTACAATTCAAAGTCCCACTAATCTAACTCTATGTGCCTCGGTCTAAATATAGGACCCTCTTGAGTTTTTTTGTTGTCCCTTTTTATAAAAAATCTTTCAAATTTTCAACTGCATTTCAACGCTACTGATATTATCAGGCAATATTAACTAATTTCAACTGCATTACACTGCTACTGATATTATCTTTCAAGTTCATTCTCTTGAGTACATCTTTTTCTGCAATCTGTAATAAATACCTTGATGAAAACATTAAGTAATTTCTCACTCTTGATCTTGAAGAATAAACTTGTAAAACAAACAATTGTCAACAAATTTAATACCACAATCAACTTTCTTAATTCCCGTGATTTACTCAACGGGGTCCCTATGAGGAGGCTGAAAAAGCTTTTGTATGACATTTACTATTAATGTTAATTGTCTATGAATAGAATAGTGTCCCAGGACAAGGTTTATTACTAAAGGAAGGGGACTTAAATAACTCTTTTCATTAAAGGAAGGGGCCTTAAATGACACTTTTCATTAGTAATCTTTCATTGTTGGTACTTCTTTGGCTAAGACCTATATGTATTTTCTTGTGGTAGCTCTGTTGTTTATGATACACTTGAGCATTGAACAAACATATACAATTTCAGAGCTGGGAGAAAACAATGGATTGTCATAGTCCAGGACAAGGTTTGATGCCTGCTAGTTTTAAGGTTCGGACAGTTCCTCTGGAAGGTGATGACTCTGCAACAGAAGAGGTTTTGGATCCTGACTTTGGAGAGGCAGCCATTGGCCGTGTTGCTCCTGTTGATTCCGGTAAAATAACTGATAGGATCTATAGAATCTTTTTTACATAACAAAAGAAACTTAACATTATACTTCTTGGATTGCTTAACTTATTTAACATGTGATCTCAGGGCTGTGGTGGATTATTTTACTACGAGCATATGGAAAATGCTCTGGGGATTTATCTGTTCAGGAGAGAGTTGATGTGCAAACAGGAATTAAGATGATTTTGAAGCTTTGTCTTGCTGATGGTTTTGACATGTTCCCAACATTATTAGTAACTGATGGTTCTTGCATGATAGATCGAAGGATGGGCATACACGGGCATCCTTTGGAGATTCAGGTGTTTACGATTTCCTGTCTTTATTCATTAGCATGCTATTTATTTAACTATTTCTTGTTAAGGTTAGACAAGAAACTTTGGTGCTTATTGATTTATTCACGACAGTGCATTTATTTAGATAGATTGGGTAAATGATACTGTTTTCTGCGATTCTGCACCATTTTGACAAGAAAAAGGAACAAGTTTTCCTATGCTAGATTGAGGACCAGCATTTCTGTCCTTTATTCTTAGTAGTGACTAGGCTCTGTCTTTTATTTTTGTTAATGGAGTTGGTATTAGGTATTTCTTATTCATTTGAAAGTGACTTAGCTATATCTTTTTTATGCATGAAAGGTAAAACCTCACATTAACCTTTATGCCCTAGGAATTTGAATGGATTAAAGATGAGGTGGGAGTGCTGGTGAATTCATATTAATAGACAGCAGCAACAGTCAATTCTGAAATAGATTGTATTGCCGTTGGATAAGACTTGTGGTAAATAAACTAAATGCAATATAAGGTTTTATAACATATTCCAAATCTGGCCCCATCAGTATGGTAGATTTTAGAAATTCAGAATACTATTGTGTTTATACAATTACACCACTGAATTGTTGAAACTACAAAAAAGGACACATCTCTTGACATAGATTTGGACTGGTATGGTACATTATCAAAAGCTTCCCATGGCTTTTACTGTTTTGAAGTAAAGGTCCCTGATGTCTCAAGGTCCCCAAATCTGTGTAATTTCACCTTTGTAAGTTCCAACAGTTAATGGTAAAAAAAAAAAAGTGAAGACACCAAAACTTTCCACCTTCCTCGTATATGTCTTACTATTCCTTCTAGTTGCCTTGGTTAAGCTATATAAATACTTTCATGCCAAGCTTTTTATATTTTGTTTTAACTTCCTGTTAAATGGGGAATCACACTGTAAAATCGTGTAGGTGTGAATGATTGGGTTTGGATTGATAGCAAATTATAAAACCCTACAGTCACGTGCATCTCAGACATTAGATTTTTTATTTTTTTTGAAAGTGCAAACACTGAGCACAAGATGCACTCGGCACGCAAAAGAGCAGTAGATACAAAGGAGAGCCTCAAAAGGACTCTAGCCAACCAAGGCACACCACAAAACAAGAACAGAAACAACTGCTCCTATCAAAGAGCAAAACCAACGCAAAAGGCAACAGGGAAAAAAAGAAACAAACTGCCAAAATAAACACAAACACACCCAGCCCACTGCTGTTAGATTTATTTAATGTCCATCAAACAGCCACGTCCCCTCTTTCTTTATATTGAAACGGCACATTTAGGAGCAACACTTCTCTTTACTCTCCCCAACTCCACCTACCTACATCGCCTTCTTGGCAATGGAAATATGTCCTCTCTTCATCATTTTGAGGACCTCCACCCACTACCATCTCAACGAAAGCTCGAGTGTGATGCCGCCTCTGCAGCCGCAACCCAAATTCTGTGTTTTTGCAGCTCGTCTGTCAATATATCAGTGACAAAGCTGTGGTTTTTTAGTAGTTGCAGAGAATCCAAATCCTGAATGGTTCTATCTTACCTAAAAAAACACTGCTAATAACTAAAAATAAATAAATTTTATGTACTATGTTTCGAAATTTTGCTTGCATGAATCACTTTTGTCAAATAAATTTATCGTTTTTATTGAAAATATTATGCTCCTGAGATGCTTACAAGTTACAATTAAAGATGGAATTAGCGATCAAATAAACTTATCTAATGTGTATTTTATTTTGCTTTCTGTTTTATTTTTCTGTTTCTAGGCACTGTTTTATTCTGCCTTACGTTGTGCACGTGAGATGCTTACTCCTGAGGATGGATCAGCTGATCTTATACGGGCACTGAACAATCGGCTGGTCGCTCTCTCATTTCATATTAGAGAATATTATTGGATCGATATGAAAAGATTAAATGAAATTTACCGTTACAAGACAGAGGAGTACTCATATGATGCAGTTAATAAATTCAACATATACCCAGACCAGATTTCTCCTTGGTTAGTGGAGTGGATGCCGAACAAAGGAGGCTACTTAATTGGTAACTTACAACCAGCTCACATGGATTTCCGGTTTTTTTCACTAGGAAACTTGTGGTCTGTTGTCAGTAGTATGGCCACAGAGGAACAATCACATGCCATATTGGATCTTATTGAGGCCAAATGGTCGGATTTGGTGGCAGATATGCCACTCAAGATTTGTTATCCGGCTCTTGAAGGTCAGGAGTGGCAGATAATCACTGGCAGTGATCCTAAGAACACGTACTGCATCTAACTTTCCTCTGCTTATGTTTTTCTGTTAAAATATTTTTCTTGAAATGATTTTATTTAATTTTTTTTGCAGGCCTTGGTCTTACCATAATGGAGGCTCCTGGCCATCACTTCTCTGGCAGGTTTGTTACAATGACAGTTTGGTTCACTTGCATCCCGAATTTTATTTTTAGATTGCAATTAGTCAGCAATGAAATGATATTGTCTGTGTATGACGAAGCAGTCGTCATTTATAGCTACTATACTTTTTTCACCTTAGCTCTTATCATAATCTGTCCTATTAAATCTTTTTCATTTAACACATGCAAATCCTTTCATAGCTTAGCTGGTTTCTTCACCTAAGCATTGCATGTTTGAATTAACACAATGTACAAAGTTTTGACATAAAAAAATGTGTATTGCAGCTCACAGCTGCATGCATAAAGATGAACAGACCACATATTGCTGCAAAAGCTGTTGAAATTGTTGAGAGACGTATATCAAGAGACAAGTGGCCAGAATATTATGACACGAAAAGATCTCGATTCATTGGAAAACAGTCTCAGTTGTTTCAGACATGGTCAATTGCGGGATACCTTGTGTCAAAGCTGCTGCTTGCAGACCCAAGTAAAGCAAACATACTGATAACTGAAGAGGATTCTGATCTTGTGATTGCTTTGATTAATGCCAACCCAAAAGGAAAGCGCGGGAGGAAAAATTTGAAGCAGACCTACATTGTATGA

Protein sequence

>MS.gene58153.t1

MSLGASKAVFQVLSSVVPQSAYNEPFVNTSQLRTNCMKKRSSRHRFLIESSSMLQSQLRPHRFPFTSVSFCDYKTYSHPWLQTCKCQKAENVSGITSGDGNGSRFASDVEKSSLVSNVMSAKSSLEFEDVQLLEQEKEVLSSNVTNGTATKNLGTISLNSIEEEAWDLLRESVVNYCGNPIGTIAAKDPNSTNVLNYDQVFIRDFIPSGVAFLLKGEYDIVRNFILHTLQLQSWEKTMDCHSPGQGLMPASFKVRTVPLEGDDSATEEVLDPDFGEAAIGRVAPVDSGLWWIILLRAYGKCSGDLSVQERVDVQTGIKMILKLCLADGFDMFPTLLVTDGSCMIDRRMGIHGHPLEIQALFYSALRCAREMLTPEDGSADLIRALNNRLVALSFHIREYYWIDMKRLNEIYRYKTEEYSYDAVNKFNIYPDQISPWLVEWMPNKGGYLIGNLQPAHMDFRFFSLGNLWSVVSSMATEEQSHAILDLIEAKWSDLVADMPLKICYPALEGQEWQIITGSDPKNTPWSYHNGGSWPSLLWQLTAACIKMNRPHIAAKAVEIVERRISRDKWPEYYDTKRSRFIGKQSQLFQTWSIAGYLVSKLLLADPSKANILITEEDSDLVIALINANPKGKRGRKNLKQTYIV