Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61138.t1 | XP_003608530.1 | 93.4 | 468 | 27 | 3 | 1 | 467 | 1 | 465 | 2.80E-267 | 930.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61138.t1 | G7JE26 | 93.4 | 468 | 27 | 3 | 1 | 467 | 1 | 465 | 2.0e-267 | 930.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049222 | MS.gene61138 | -0.811283 | 7.56E-51 | -1.69E-46 |
| MS.gene049232 | MS.gene61138 | -0.824226 | 9.05E-54 | -1.69E-46 |
| MS.gene049320 | MS.gene61138 | -0.808434 | 3.10E-50 | -1.69E-46 |
| MS.gene049633 | MS.gene61138 | -0.814583 | 1.43E-51 | -1.69E-46 |
| MS.gene05010 | MS.gene61138 | -0.82472 | 6.93E-54 | -1.69E-46 |
| MS.gene050170 | MS.gene61138 | -0.851005 | 1.18E-60 | -1.69E-46 |
| MS.gene050323 | MS.gene61138 | -0.840112 | 1.06E-57 | -1.69E-46 |
| MS.gene050338 | MS.gene61138 | 0.816721 | 4.78E-52 | -1.69E-46 |
| MS.gene050496 | MS.gene61138 | -0.839111 | 1.93E-57 | -1.69E-46 |
| MS.gene050502 | MS.gene61138 | -0.802095 | 6.60E-49 | -1.69E-46 |
| MS.gene050504 | MS.gene61138 | -0.813278 | 2.78E-51 | -1.69E-46 |
| MS.gene050577 | MS.gene61138 | -0.815667 | 8.22E-52 | -1.69E-46 |
| MS.gene050612 | MS.gene61138 | -0.829959 | 3.84E-55 | -1.69E-46 |
| MS.gene050675 | MS.gene61138 | -0.806722 | 7.17E-50 | -1.69E-46 |
| MS.gene050697 | MS.gene61138 | -0.831412 | 1.69E-55 | -1.69E-46 |
| MS.gene05080 | MS.gene61138 | -0.807133 | 5.87E-50 | -1.69E-46 |
| MS.gene050868 | MS.gene61138 | -0.872412 | 3.24E-67 | -1.69E-46 |
| MS.gene050871 | MS.gene61138 | -0.872658 | 2.69E-67 | -1.69E-46 |
| MS.gene050904 | MS.gene61138 | -0.805546 | 1.27E-49 | -1.69E-46 |
| MS.gene050986 | MS.gene61138 | -0.808997 | 2.35E-50 | -1.69E-46 |
| MS.gene05104 | MS.gene61138 | 0.801652 | 8.14E-49 | -1.69E-46 |
| MS.gene051138 | MS.gene61138 | 0.827767 | 1.30E-54 | -1.69E-46 |
| MS.gene051268 | MS.gene61138 | -0.846729 | 1.81E-59 | -1.69E-46 |
| MS.gene051317 | MS.gene61138 | -0.806572 | 7.71E-50 | -1.69E-46 |
| MS.gene051439 | MS.gene61138 | 0.827835 | 1.26E-54 | -1.69E-46 |
| MS.gene051651 | MS.gene61138 | 0.815586 | 8.57E-52 | -1.69E-46 |
| MS.gene051838 | MS.gene61138 | -0.812533 | 4.04E-51 | -1.69E-46 |
| MS.gene051863 | MS.gene61138 | 0.839175 | 1.85E-57 | -1.69E-46 |
| MS.gene051874 | MS.gene61138 | -0.806284 | 8.87E-50 | -1.69E-46 |
| MS.gene051941 | MS.gene61138 | -0.818251 | 2.16E-52 | -1.69E-46 |
| MS.gene052164 | MS.gene61138 | -0.812001 | 5.28E-51 | -1.69E-46 |
| MS.gene052482 | MS.gene61138 | -0.806263 | 8.96E-50 | -1.69E-46 |
| MS.gene052486 | MS.gene61138 | -0.806859 | 6.70E-50 | -1.69E-46 |
| MS.gene052503 | MS.gene61138 | -0.803651 | 3.15E-49 | -1.69E-46 |
| MS.gene052823 | MS.gene61138 | -0.809686 | 1.67E-50 | -1.69E-46 |
| MS.gene052825 | MS.gene61138 | -0.800614 | 1.33E-48 | -1.69E-46 |
| MS.gene052966 | MS.gene61138 | -0.8142 | 1.74E-51 | -1.69E-46 |
| MS.gene053203 | MS.gene61138 | -0.828209 | 1.02E-54 | -1.69E-46 |
| MS.gene053420 | MS.gene61138 | 0.809216 | 2.11E-50 | -1.69E-46 |
| MS.gene053421 | MS.gene61138 | 0.804844 | 1.78E-49 | -1.69E-46 |
| MS.gene053479 | MS.gene61138 | -0.831939 | 1.26E-55 | -1.69E-46 |
| MS.gene053480 | MS.gene61138 | -0.828148 | 1.06E-54 | -1.69E-46 |
| MS.gene053824 | MS.gene61138 | -0.825751 | 3.95E-54 | -1.69E-46 |
| MS.gene054003 | MS.gene61138 | -0.800326 | 1.52E-48 | -1.69E-46 |
| MS.gene054595 | MS.gene61138 | -0.811208 | 7.85E-51 | -1.69E-46 |
| MS.gene054706 | MS.gene61138 | 0.834711 | 2.56E-56 | -1.69E-46 |
| MS.gene05470 | MS.gene61138 | -0.820618 | 6.23E-53 | -1.69E-46 |
| MS.gene05494 | MS.gene61138 | -0.800519 | 1.39E-48 | -1.69E-46 |
| MS.gene054954 | MS.gene61138 | -0.810258 | 1.26E-50 | -1.69E-46 |
| MS.gene055502 | MS.gene61138 | -0.804964 | 1.68E-49 | -1.69E-46 |
| MS.gene055577 | MS.gene61138 | -0.814524 | 1.47E-51 | -1.69E-46 |
| MS.gene055748 | MS.gene61138 | -0.802137 | 6.47E-49 | -1.69E-46 |
| MS.gene055750 | MS.gene61138 | -0.812671 | 3.77E-51 | -1.69E-46 |
| MS.gene055751 | MS.gene61138 | -0.813415 | 2.59E-51 | -1.69E-46 |
| MS.gene05589 | MS.gene61138 | -0.860217 | 2.41E-63 | -1.69E-46 |
| MS.gene056046 | MS.gene61138 | -0.802642 | 5.09E-49 | -1.69E-46 |
| MS.gene056387 | MS.gene61138 | -0.887253 | 1.68E-72 | -1.69E-46 |
| MS.gene056504 | MS.gene61138 | -0.859593 | 3.71E-63 | -1.69E-46 |
| MS.gene056618 | MS.gene61138 | 0.808132 | 3.60E-50 | -1.69E-46 |
| MS.gene056697 | MS.gene61138 | 0.810201 | 1.30E-50 | -1.69E-46 |
| MS.gene057086 | MS.gene61138 | -0.831511 | 1.60E-55 | -1.69E-46 |
| MS.gene057768 | MS.gene61138 | 0.807018 | 6.20E-50 | -1.69E-46 |
| MS.gene057857 | MS.gene61138 | -0.804386 | 2.21E-49 | -1.69E-46 |
| MS.gene05787 | MS.gene61138 | -0.82253 | 2.26E-53 | -1.69E-46 |
| MS.gene058099 | MS.gene61138 | 0.815526 | 8.84E-52 | -1.69E-46 |
| MS.gene058109 | MS.gene61138 | 0.800145 | 1.65E-48 | -1.69E-46 |
| MS.gene058157 | MS.gene61138 | -0.830772 | 2.43E-55 | -1.69E-46 |
| MS.gene058171 | MS.gene61138 | 0.835711 | 1.43E-56 | -1.69E-46 |
| MS.gene058173 | MS.gene61138 | 0.835085 | 2.06E-56 | -1.69E-46 |
| MS.gene058175 | MS.gene61138 | 0.842338 | 2.75E-58 | -1.69E-46 |
| MS.gene058268 | MS.gene61138 | 0.804868 | 1.76E-49 | -1.69E-46 |
| MS.gene058334 | MS.gene61138 | 0.805271 | 1.45E-49 | -1.69E-46 |
| MS.gene05837 | MS.gene61138 | -0.816732 | 4.75E-52 | -1.69E-46 |
| MS.gene059023 | MS.gene61138 | -0.807532 | 4.83E-50 | -1.69E-46 |
| MS.gene059045 | MS.gene61138 | 0.809011 | 2.34E-50 | -1.69E-46 |
| MS.gene059223 | MS.gene61138 | 0.811449 | 6.96E-51 | -1.69E-46 |
| MS.gene05926 | MS.gene61138 | 0.822695 | 2.06E-53 | -1.69E-46 |
| MS.gene059367 | MS.gene61138 | -0.80688 | 6.63E-50 | -1.69E-46 |
| MS.gene059667 | MS.gene61138 | -0.812464 | 4.18E-51 | -1.69E-46 |
| MS.gene059699 | MS.gene61138 | 0.826941 | 2.06E-54 | -1.69E-46 |
| MS.gene059703 | MS.gene61138 | 0.830613 | 2.66E-55 | -1.69E-46 |
| MS.gene059865 | MS.gene61138 | -0.846035 | 2.80E-59 | -1.69E-46 |
| MS.gene06000 | MS.gene61138 | -0.873619 | 1.28E-67 | -1.69E-46 |
| MS.gene060405 | MS.gene61138 | 0.801532 | 8.61E-49 | -1.69E-46 |
| MS.gene060595 | MS.gene61138 | 0.810405 | 1.17E-50 | -1.69E-46 |
| MS.gene060762 | MS.gene61138 | -0.819559 | 1.09E-52 | -1.69E-46 |
| MS.gene060778 | MS.gene61138 | -0.851553 | 8.24E-61 | -1.69E-46 |
| MS.gene06077 | MS.gene61138 | -0.817871 | 2.63E-52 | -1.69E-46 |
| MS.gene060823 | MS.gene61138 | 0.800745 | 1.25E-48 | -1.69E-46 |
| MS.gene06097 | MS.gene61138 | -0.803984 | 2.68E-49 | -1.69E-46 |
| MS.gene060982 | MS.gene61138 | -0.852042 | 5.98E-61 | -1.69E-46 |
| MS.gene061218 | MS.gene61138 | -0.803534 | 3.33E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61138.t1 | MTR_4g097110 | 93.376 | 468 | 27 | 3 | 1 | 467 | 1 | 465 | 0.0 | 880 |
| MS.gene61138.t1 | MTR_5g015460 | 66.876 | 477 | 142 | 7 | 1 | 468 | 1 | 470 | 0.0 | 651 |
| MS.gene61138.t1 | MTR_5g015460 | 72.019 | 411 | 105 | 4 | 63 | 468 | 62 | 467 | 0.0 | 636 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61138.t1 | AT3G49880 | 64.043 | 470 | 143 | 7 | 3 | 461 | 4 | 458 | 0.0 | 611 |
| MS.gene61138.t1 | AT5G67540 | 62.900 | 469 | 146 | 8 | 12 | 461 | 12 | 471 | 0.0 | 598 |
| MS.gene61138.t1 | AT5G67540 | 62.900 | 469 | 146 | 8 | 12 | 461 | 34 | 493 | 0.0 | 598 |
| MS.gene61138.t1 | AT5G67540 | 63.191 | 470 | 142 | 10 | 12 | 461 | 8 | 466 | 0.0 | 597 |
Find 95 sgRNAs with CRISPR-Local
Find 155 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTCAGCTGAGACAATCTT+AGG | 0.186965 | 8.1:+25509691 | MS.gene61138:CDS |
| AAATTTCAAATACTTATTAT+TGG | 0.207042 | 8.1:+25508283 | MS.gene61138:CDS |
| TCCTGGTTTGTTTATCTTCA+TGG | 0.221019 | 8.1:+25509816 | MS.gene61138:CDS |
| CTATGTCACTAGTAATAATA+TGG | 0.224189 | 8.1:+25507881 | MS.gene61138:CDS |
| ACTGCTACCCTGGGAGAATT+TGG | 0.230166 | 8.1:+25508205 | MS.gene61138:CDS |
| GCTATTCTTCTAAGGATCTA+TGG | 0.248673 | 8.1:+25509188 | MS.gene61138:CDS |
| TTGCAACGAAAATTCGTAAT+TGG | 0.249294 | 8.1:-25507838 | None:intergenic |
| ACCATTGGTTGATGAATTCT+TGG | 0.278506 | 8.1:+25508083 | MS.gene61138:CDS |
| TTTCAACTTGTTGGAGTTGA+TGG | 0.278724 | 8.1:-25507999 | None:intergenic |
| GGCCAAGAGTTTCTATCTAT+TGG | 0.280101 | 8.1:+25509950 | MS.gene61138:CDS |
| TTCTCATTGTAAATAACTTT+TGG | 0.283365 | 8.1:-25509295 | None:intergenic |
| CTTCCTTTACCTAAGTTTCC+TGG | 0.285443 | 8.1:+25509799 | MS.gene61138:CDS |
| TTTGATATCCATGCGGTCTT+TGG | 0.289529 | 8.1:-25509429 | None:intergenic |
| AGTTGGTTGCTATTCTTCTA+AGG | 0.296029 | 8.1:+25509180 | MS.gene61138:CDS |
| ATATGGGGTTTATTAATCTT+AGG | 0.298477 | 8.1:+25507898 | MS.gene61138:CDS |
| ATTGATCCAATGAAGACTAT+AGG | 0.340719 | 8.1:+25508156 | MS.gene61138:CDS |
| CCTTTCCCTATAGTCTTCAT+TGG | 0.349246 | 8.1:-25508162 | None:intergenic |
| GAAGATCAAGCACTTCAATA+TGG | 0.356355 | 8.1:+25509910 | MS.gene61138:CDS |
| CTCCATCAGGTGGACATTAT+AGG | 0.359766 | 8.1:+25509157 | MS.gene61138:intron |
| TGGTTGCCTTTGATTGTTGA+TGG | 0.375658 | 8.1:+25509883 | MS.gene61138:CDS |
| TTATCTTCATGGCGGATAGA+TGG | 0.376808 | 8.1:+25509827 | MS.gene61138:CDS |
| TAAGAGATTCAAGATATGTT+TGG | 0.401561 | 8.1:+25509863 | MS.gene61138:CDS |
| TTGATCCAATGAAGACTATA+GGG | 0.410737 | 8.1:+25508157 | MS.gene61138:CDS |
| CTTGAATCTCTTAACTCACT+TGG | 0.414129 | 8.1:-25509853 | None:intergenic |
| CCTTTGTGTTTGAACATAGC+CGG | 0.433704 | 8.1:-25509610 | None:intergenic |
| TCAAATACTTATTATTGGTA+TGG | 0.434316 | 8.1:+25508288 | MS.gene61138:CDS |
| AGGAGGATGAATGATATTGA+AGG | 0.445423 | 8.1:-25507959 | None:intergenic |
| TTAGGGACATGGGAAACAAT+TGG | 0.448046 | 8.1:+25509709 | MS.gene61138:CDS |
| TTATTATGAGCAAGGTAAGT+AGG | 0.456459 | 8.1:-25508327 | None:intergenic |
| TGGCTACCAAGGTATTTGAA+TGG | 0.456566 | 8.1:-25509409 | None:intergenic |
| TATGTCACTAGTAATAATAT+GGG | 0.459282 | 8.1:+25507882 | MS.gene61138:CDS |
| ATAAACAAACCAGGAAACTT+AGG | 0.469675 | 8.1:-25509808 | None:intergenic |
| CACCCTCCATGAGCTTGAAT+AGG | 0.470225 | 8.1:-25508246 | None:intergenic |
| AGTTATAATTACTGCTACCC+TGG | 0.470470 | 8.1:+25508195 | MS.gene61138:CDS |
| TCAACTCCAACAAGTTGAAA+AGG | 0.485321 | 8.1:+25508002 | MS.gene61138:CDS |
| ACATATTACATGGTTACATC+AGG | 0.501941 | 8.1:+25509634 | MS.gene61138:CDS |
| GTCCTTTGTTATTATGAGCA+AGG | 0.505074 | 8.1:-25508335 | None:intergenic |
| GGAAATCCTATTCAAGCTCA+TGG | 0.506131 | 8.1:+25508240 | MS.gene61138:CDS |
| CAGGTGGACATTATAGGAGT+TGG | 0.509259 | 8.1:+25509163 | MS.gene61138:intron |
| CTACCCTGGGAGAATTTGGT+TGG | 0.510007 | 8.1:+25508209 | MS.gene61138:CDS |
| GAATGAACAAGTGCTTCATT+TGG | 0.511215 | 8.1:-25509673 | None:intergenic |
| ATGGTTACATCAGGTTGCAC+TGG | 0.512390 | 8.1:+25509643 | MS.gene61138:CDS |
| GACCGCATGGATATCAAAGC+CGG | 0.513956 | 8.1:+25509434 | MS.gene61138:CDS |
| AATGGAAACTGCCTTTGGGT+TGG | 0.518583 | 8.1:+25509980 | MS.gene61138:CDS |
| CTGAGACAATCTTAGGGACA+TGG | 0.522359 | 8.1:+25509698 | MS.gene61138:CDS |
| TTACATCAGGTTGCACTGGA+TGG | 0.529710 | 8.1:+25509647 | MS.gene61138:CDS |
| TATTATGAGCAAGGTAAGTA+GGG | 0.532830 | 8.1:-25508326 | None:intergenic |
| AAACCAGGAAACTTAGGTAA+AGG | 0.536263 | 8.1:-25509802 | None:intergenic |
| TACATCAGGTTGCACTGGAT+GGG | 0.537898 | 8.1:+25509648 | MS.gene61138:CDS |
| CTTTGAAAAGTGTCATGTCC+CGG | 0.538558 | 8.1:-25509453 | None:intergenic |
| AGAATTTGGTTGGATACTGA+TGG | 0.538862 | 8.1:+25508219 | MS.gene61138:CDS |
| GGTGTTATTGACTTGCTTTG+AGG | 0.541852 | 8.1:-25508063 | None:intergenic |
| TGCCAATAGATAGAAACTCT+TGG | 0.542445 | 8.1:-25509952 | None:intergenic |
| TGGAAATGAGAAGGAAGAGA+AGG | 0.543749 | 8.1:-25507979 | None:intergenic |
| TGGTATGGTGAATATAAAGA+TGG | 0.548966 | 8.1:+25508303 | MS.gene61138:CDS |
| AAATGAGAAGGAAGAGAAGG+AGG | 0.550677 | 8.1:-25507976 | None:intergenic |
| TCATGTCCATCAACAATCAA+AGG | 0.551449 | 8.1:-25509889 | None:intergenic |
| ATTGGAAATCCATGTGTTGG+TGG | 0.555806 | 8.1:+25509727 | MS.gene61138:CDS |
| AAGCAAGTCAATAACACCAT+TGG | 0.559003 | 8.1:+25508068 | MS.gene61138:CDS |
| ATTCAGCTGAGACAATCTTA+GGG | 0.560376 | 8.1:+25509692 | MS.gene61138:CDS |
| TGAAAGTGACTTACACGAGC+AGG | 0.561123 | 8.1:-25508357 | None:intergenic |
| CTTCTAAGGATCTATGGACC+TGG | 0.567240 | 8.1:+25509194 | MS.gene61138:CDS |
| GTTATAATTACTGCTACCCT+GGG | 0.579486 | 8.1:+25508196 | MS.gene61138:CDS |
| GTATCCAACCAAATTCTCCC+AGG | 0.580663 | 8.1:-25508213 | None:intergenic |
| ACCGGTCCATTCAAATACCT+TGG | 0.583019 | 8.1:+25509403 | MS.gene61138:CDS |
| TTGAGATAATCTTCAGTCAA+GGG | 0.584839 | 8.1:-25509538 | None:intergenic |
| ACCAAGGTATTTGAATGGAC+CGG | 0.587941 | 8.1:-25509404 | None:intergenic |
| AGGATGAATGATATTGAAGG+AGG | 0.591474 | 8.1:-25507956 | None:intergenic |
| TTGAATGTGTTCCAACCCAA+AGG | 0.592407 | 8.1:-25509991 | None:intergenic |
| ATTGAGATAATCTTCAGTCA+AGG | 0.592919 | 8.1:-25509539 | None:intergenic |
| GAAAACAATGTAATGCACAT+TGG | 0.594015 | 8.1:+25509514 | MS.gene61138:CDS |
| ACCGCATGGATATCAAAGCC+GGG | 0.595132 | 8.1:+25509435 | MS.gene61138:CDS |
| CGGTCAGCGAAGAGAAGCGC+CGG | 0.596526 | 8.1:+25509591 | MS.gene61138:CDS |
| CATGCGGTCTTTGGCTACCA+AGG | 0.596927 | 8.1:-25509420 | None:intergenic |
| GCATTCAGTGATACTCCTAC+CGG | 0.598208 | 8.1:+25509385 | MS.gene61138:CDS |
| CCAATGAAGACTATAGGGAA+AGG | 0.603987 | 8.1:+25508162 | MS.gene61138:CDS |
| TACCTTGCTCATAATAACAA+AGG | 0.607221 | 8.1:+25508333 | MS.gene61138:CDS |
| ACACAAAGGAACATATTACA+TGG | 0.610656 | 8.1:+25509624 | MS.gene61138:CDS |
| ATCCTATTCAAGCTCATGGA+GGG | 0.611296 | 8.1:+25508244 | MS.gene61138:CDS |
| CTTGGTAGCCAAAGACCGCA+TGG | 0.613799 | 8.1:+25509421 | MS.gene61138:CDS |
| AAACAAGAAAGTATGTGATG+TGG | 0.618490 | 8.1:+25509317 | MS.gene61138:CDS |
| TGAGACAATCTTAGGGACAT+GGG | 0.620421 | 8.1:+25509699 | MS.gene61138:CDS |
| TCCAAGAATTCATCAACCAA+TGG | 0.620834 | 8.1:-25508084 | None:intergenic |
| ATCACTGAATGCTATGCCGA+CGG | 0.621301 | 8.1:-25509374 | None:intergenic |
| TGGAGTTGATGGAAATGAGA+AGG | 0.629494 | 8.1:-25507988 | None:intergenic |
| ACAATTGGAAATCCATGTGT+TGG | 0.635790 | 8.1:+25509724 | MS.gene61138:CDS |
| ATGTCACTAGTAATAATATG+GGG | 0.640588 | 8.1:+25507883 | MS.gene61138:CDS |
| TGGTTTGTTTATCTTCATGG+CGG | 0.648154 | 8.1:+25509819 | MS.gene61138:CDS |
| AGGTATTTGAATGGACCGGT+AGG | 0.664578 | 8.1:-25509400 | None:intergenic |
| TATCCAACCAAATTCTCCCA+GGG | 0.669311 | 8.1:-25508212 | None:intergenic |
| CCGGCTATGTTCAAACACAA+AGG | 0.678714 | 8.1:+25509610 | MS.gene61138:CDS |
| AATTACGCAAAAGCTTCCGT+CGG | 0.686419 | 8.1:+25509358 | MS.gene61138:CDS |
| AATCCTATTCAAGCTCATGG+AGG | 0.687704 | 8.1:+25508243 | MS.gene61138:CDS |
| GCCATGAAGATAAACAAACC+AGG | 0.690008 | 8.1:-25509817 | None:intergenic |
| TCCCGGCTTTGATATCCATG+CGG | 0.711426 | 8.1:-25509436 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTTCAAATACTTATTAT+TGG | + | chr8.1:25508283-25508302 | MS.gene61138:CDS | 10.0% |
| !! | GTAAAATGTAAGTTATAATA+AGG | - | chr8.1:25508577-25508596 | None:intergenic | 15.0% |
| !! | ATGAAAAGAACATTTGATAT+GGG | - | chr8.1:25508719-25508738 | None:intergenic | 20.0% |
| !! | TAACATACTTCTATAACAAA+AGG | - | chr8.1:25508599-25508618 | None:intergenic | 20.0% |
| !! | TATGTCACTAGTAATAATAT+GGG | + | chr8.1:25507882-25507901 | MS.gene61138:CDS | 20.0% |
| !! | TCAAATACTTATTATTGGTA+TGG | + | chr8.1:25508288-25508307 | MS.gene61138:CDS | 20.0% |
| !!! | ATTAAGTTGTTTATTCAATC+AGG | + | chr8.1:25508648-25508667 | MS.gene61138:intron | 20.0% |
| !!! | TTAAGTTGTTTATTCAATCA+GGG | + | chr8.1:25508649-25508668 | MS.gene61138:intron | 20.0% |
| !!! | TTCTCATTGTAAATAACTTT+TGG | - | chr8.1:25509298-25509317 | None:intergenic | 20.0% |
| ! | AACCATAAGAAACTTTGAAT+TGG | - | chr8.1:25508742-25508761 | None:intergenic | 25.0% |
| ! | ATATGGGGTTTATTAATCTT+AGG | + | chr8.1:25507898-25507917 | MS.gene61138:CDS | 25.0% |
| ! | ATCCAATTCAAAGTTTCTTA+TGG | + | chr8.1:25508737-25508756 | MS.gene61138:intron | 25.0% |
| ! | ATGTCACTAGTAATAATATG+GGG | + | chr8.1:25507883-25507902 | MS.gene61138:CDS | 25.0% |
| ! | CCAAAAACTTCAATTGATAA+TGG | - | chr8.1:25508962-25508981 | None:intergenic | 25.0% |
| ! | CTATCTATTGGCATAAAAAA+TGG | + | chr8.1:25509962-25509981 | MS.gene61138:CDS | 25.0% |
| ! | CTATGTCACTAGTAATAATA+TGG | + | chr8.1:25507881-25507900 | MS.gene61138:CDS | 25.0% |
| ! | GATGAAAAGAACATTTGATA+TGG | - | chr8.1:25508720-25508739 | None:intergenic | 25.0% |
| ! | GGAAATTGTATTTGTTTCAT+AGG | + | chr8.1:25508985-25509004 | MS.gene61138:intron | 25.0% |
| ! | TAAGAGATTCAAGATATGTT+TGG | + | chr8.1:25509863-25509882 | MS.gene61138:CDS | 25.0% |
| !! | ATCAAATGCAAATACTGTTT+TGG | + | chr8.1:25509103-25509122 | MS.gene61138:intron | 25.0% |
| !! | CCATTATCAATTGAAGTTTT+TGG | + | chr8.1:25508959-25508978 | MS.gene61138:intron | 25.0% |
| !!! | AAAACTTCAATTGATAATGG+TGG | - | chr8.1:25508959-25508978 | None:intergenic | 25.0% |
| !!! | ATGGTTTTGATAACACATTT+TGG | + | chr8.1:25509929-25509948 | MS.gene61138:CDS | 25.0% |
| !!! | CTTATTTTTATAAGCTCTTC+AGG | + | chr8.1:25508535-25508554 | MS.gene61138:intron | 25.0% |
| AAAAAATGGAAACTGCCTTT+GGG | + | chr8.1:25509976-25509995 | MS.gene61138:CDS | 30.0% | |
| AAAGCATATACACATGTCAT+AGG | - | chr8.1:25508506-25508525 | None:intergenic | 30.0% | |
| ACACAAAGGAACATATTACA+TGG | + | chr8.1:25509624-25509643 | MS.gene61138:CDS | 30.0% | |
| ACATATTACATGGTTACATC+AGG | + | chr8.1:25509634-25509653 | MS.gene61138:CDS | 30.0% | |
| ATAAACAAACCAGGAAACTT+AGG | - | chr8.1:25509811-25509830 | None:intergenic | 30.0% | |
| ATTGAGATAATCTTCAGTCA+AGG | - | chr8.1:25509542-25509561 | None:intergenic | 30.0% | |
| ATTGATCCAATGAAGACTAT+AGG | + | chr8.1:25508156-25508175 | MS.gene61138:CDS | 30.0% | |
| CTTATCTACTGACATGAATA+CGG | + | chr8.1:25508448-25508467 | MS.gene61138:intron | 30.0% | |
| GAAAACAATGTAATGCACAT+TGG | + | chr8.1:25509514-25509533 | MS.gene61138:CDS | 30.0% | |
| TAAAAAATGGAAACTGCCTT+TGG | + | chr8.1:25509975-25509994 | MS.gene61138:CDS | 30.0% | |
| TACCTTGCTCATAATAACAA+AGG | + | chr8.1:25508333-25508352 | MS.gene61138:CDS | 30.0% | |
| TATTATGAGCAAGGTAAGTA+GGG | - | chr8.1:25508329-25508348 | None:intergenic | 30.0% | |
| TGGTATGGTGAATATAAAGA+TGG | + | chr8.1:25508303-25508322 | MS.gene61138:CDS | 30.0% | |
| TTATCTACTGACATGAATAC+GGG | + | chr8.1:25508449-25508468 | MS.gene61138:intron | 30.0% | |
| TTATTATGAGCAAGGTAAGT+AGG | - | chr8.1:25508330-25508349 | None:intergenic | 30.0% | |
| TTGAGATAATCTTCAGTCAA+GGG | - | chr8.1:25509541-25509560 | None:intergenic | 30.0% | |
| TTGATCCAATGAAGACTATA+GGG | + | chr8.1:25508157-25508176 | MS.gene61138:CDS | 30.0% | |
| TTGCAACGAAAATTCGTAAT+TGG | - | chr8.1:25507841-25507860 | None:intergenic | 30.0% | |
| TTTGTTTCATAGGACTAATG+TGG | + | chr8.1:25508995-25509014 | MS.gene61138:intron | 30.0% | |
| ! | AAACAAGAAAGTATGTGATG+TGG | + | chr8.1:25509317-25509336 | MS.gene61138:CDS | 30.0% |
| ! | GTCATGAAAAGAATTTTCGT+CGG | + | chr8.1:25509571-25509590 | MS.gene61138:CDS | 30.0% |
| ! | TGAGATATAGACTAGAGTTT+TGG | - | chr8.1:25509129-25509148 | None:intergenic | 30.0% |
| !!! | ATCAATTGAAGTTTTTGGCA+GGG | + | chr8.1:25508964-25508983 | MS.gene61138:intron | 30.0% |
| !!! | TACAATACCTTCTTTTTTCC+AGG | - | chr8.1:25509215-25509234 | None:intergenic | 30.0% |
| !!! | TATCAATTGAAGTTTTTGGC+AGG | + | chr8.1:25508963-25508982 | MS.gene61138:intron | 30.0% |
| !!! | TCAATAGCACTTGTTTTATG+AGG | - | chr8.1:25508141-25508160 | None:intergenic | 30.0% |
| AAACCAGGAAACTTAGGTAA+AGG | - | chr8.1:25509805-25509824 | None:intergenic | 35.0% | |
| AAGCAAGTCAATAACACCAT+TGG | + | chr8.1:25508068-25508087 | MS.gene61138:CDS | 35.0% | |
| ACAATTGGAAATCCATGTGT+TGG | + | chr8.1:25509724-25509743 | MS.gene61138:CDS | 35.0% | |
| AGAATTTGGTTGGATACTGA+TGG | + | chr8.1:25508219-25508238 | MS.gene61138:CDS | 35.0% | |
| AGACATTGGAATTGCTCAAT+TGG | - | chr8.1:25508908-25508927 | None:intergenic | 35.0% | |
| AGGAGGATGAATGATATTGA+AGG | - | chr8.1:25507962-25507981 | None:intergenic | 35.0% | |
| AGGATGAATGATATTGAAGG+AGG | - | chr8.1:25507959-25507978 | None:intergenic | 35.0% | |
| AGTTATAATTACTGCTACCC+TGG | + | chr8.1:25508195-25508214 | MS.gene61138:CDS | 35.0% | |
| ATTCAGCTGAGACAATCTTA+GGG | + | chr8.1:25509692-25509711 | MS.gene61138:CDS | 35.0% | |
| CTTGAATCTCTTAACTCACT+TGG | - | chr8.1:25509856-25509875 | None:intergenic | 35.0% | |
| GAAGATCAAGCACTTCAATA+TGG | + | chr8.1:25509910-25509929 | MS.gene61138:CDS | 35.0% | |
| GAATGAACAAGTGCTTCATT+TGG | - | chr8.1:25509676-25509695 | None:intergenic | 35.0% | |
| GTTATAATTACTGCTACCCT+GGG | + | chr8.1:25508196-25508215 | MS.gene61138:CDS | 35.0% | |
| TAGTTCAGAACTCAAGACAT+TGG | - | chr8.1:25508922-25508941 | None:intergenic | 35.0% | |
| TATAAACTCCTACAACCAAC+TGG | - | chr8.1:25508687-25508706 | None:intergenic | 35.0% | |
| TCATGTCCATCAACAATCAA+AGG | - | chr8.1:25509892-25509911 | None:intergenic | 35.0% | |
| TCCAAGAATTCATCAACCAA+TGG | - | chr8.1:25508087-25508106 | None:intergenic | 35.0% | |
| TGCCAATAGATAGAAACTCT+TGG | - | chr8.1:25509955-25509974 | None:intergenic | 35.0% | |
| ! | AGTTCTCCTTTTCAACTTGT+TGG | - | chr8.1:25508011-25508030 | None:intergenic | 35.0% |
| ! | AGTTGGTTGCTATTCTTCTA+AGG | + | chr8.1:25509180-25509199 | MS.gene61138:CDS | 35.0% |
| ! | GCTATTCTTCTAAGGATCTA+TGG | + | chr8.1:25509188-25509207 | MS.gene61138:CDS | 35.0% |
| ! | TCAACTCCAACAAGTTGAAA+AGG | + | chr8.1:25508002-25508021 | MS.gene61138:CDS | 35.0% |
| !! | ACCATTGGTTGATGAATTCT+TGG | + | chr8.1:25508083-25508102 | MS.gene61138:CDS | 35.0% |
| !! | ATTTTGTTTCCACCAACACA+TGG | - | chr8.1:25509739-25509758 | None:intergenic | 35.0% |
| !! | GTCCTTTGTTATTATGAGCA+AGG | - | chr8.1:25508338-25508357 | None:intergenic | 35.0% |
| !! | TCAATTGATAATGGTGGTGA+TGG | - | chr8.1:25508953-25508972 | None:intergenic | 35.0% |
| !! | TCCTGGTTTGTTTATCTTCA+TGG | + | chr8.1:25509816-25509835 | MS.gene61138:CDS | 35.0% |
| !! | TGGTTTGTTTATCTTCATGG+CGG | + | chr8.1:25509819-25509838 | MS.gene61138:CDS | 35.0% |
| !! | TTTCAACTTGTTGGAGTTGA+TGG | - | chr8.1:25508002-25508021 | None:intergenic | 35.0% |
| AAATGAGAAGGAAGAGAAGG+AGG | - | chr8.1:25507979-25507998 | None:intergenic | 40.0% | |
| AATCCTATTCAAGCTCATGG+AGG | + | chr8.1:25508243-25508262 | MS.gene61138:CDS | 40.0% | |
| AATTACGCAAAAGCTTCCGT+CGG | + | chr8.1:25509358-25509377 | MS.gene61138:CDS | 40.0% | |
| ACATGTCTAGATAGCTAGCT+TGG | - | chr8.1:25508864-25508883 | None:intergenic | 40.0% | |
| ATCCTATTCAAGCTCATGGA+GGG | + | chr8.1:25508244-25508263 | MS.gene61138:CDS | 40.0% | |
| ATTGGAAATCCATGTGTTGG+TGG | + | chr8.1:25509727-25509746 | MS.gene61138:CDS | 40.0% | |
| CATTCAGCTGAGACAATCTT+AGG | + | chr8.1:25509691-25509710 | MS.gene61138:CDS | 40.0% | |
| CCAATGAAGACTATAGGGAA+AGG | + | chr8.1:25508162-25508181 | MS.gene61138:CDS | 40.0% | |
| CCTTTCCCTATAGTCTTCAT+TGG | - | chr8.1:25508165-25508184 | None:intergenic | 40.0% | |
| CCTTTGTGTTTGAACATAGC+CGG | - | chr8.1:25509613-25509632 | None:intergenic | 40.0% | |
| CTATGGACCTGGAAAAAAGA+AGG | + | chr8.1:25509205-25509224 | MS.gene61138:CDS | 40.0% | |
| CTTCCTTTACCTAAGTTTCC+TGG | + | chr8.1:25509799-25509818 | MS.gene61138:CDS | 40.0% | |
| GCCATGAAGATAAACAAACC+AGG | - | chr8.1:25509820-25509839 | None:intergenic | 40.0% | |
| GGAAATCCTATTCAAGCTCA+TGG | + | chr8.1:25508240-25508259 | MS.gene61138:CDS | 40.0% | |
| GGCCAAGAGTTTCTATCTAT+TGG | + | chr8.1:25509950-25509969 | MS.gene61138:CDS | 40.0% | |
| TATCCAACCAAATTCTCCCA+GGG | - | chr8.1:25508215-25508234 | None:intergenic | 40.0% | |
| TGAGACAATCTTAGGGACAT+GGG | + | chr8.1:25509699-25509718 | MS.gene61138:CDS | 40.0% | |
| TGGAAATGAGAAGGAAGAGA+AGG | - | chr8.1:25507982-25508001 | None:intergenic | 40.0% | |
| TGGTTGCCTTTGATTGTTGA+TGG | + | chr8.1:25509883-25509902 | MS.gene61138:CDS | 40.0% | |
| TTAGGGACATGGGAAACAAT+TGG | + | chr8.1:25509709-25509728 | MS.gene61138:CDS | 40.0% | |
| TTGAATGTGTTCCAACCCAA+AGG | - | chr8.1:25509994-25510013 | None:intergenic | 40.0% | |
| TTTGATATCCATGCGGTCTT+TGG | - | chr8.1:25509432-25509451 | None:intergenic | 40.0% | |
| ! | ACCAAGGTATTTGAATGGAC+CGG | - | chr8.1:25509407-25509426 | None:intergenic | 40.0% |
| ! | AGCTAGCTTGGGGAAAATAA+GGG | - | chr8.1:25508852-25508871 | None:intergenic | 40.0% |
| ! | ATGTCTAGATAGCTAGCTTG+GGG | - | chr8.1:25508862-25508881 | None:intergenic | 40.0% |
| ! | CATGTCTAGATAGCTAGCTT+GGG | - | chr8.1:25508863-25508882 | None:intergenic | 40.0% |
| ! | TAGCTAGCTTGGGGAAAATA+AGG | - | chr8.1:25508853-25508872 | None:intergenic | 40.0% |
| ! | TGAGGTGACCTCTTTTTGTT+TGG | - | chr8.1:25508048-25508067 | None:intergenic | 40.0% |
| ! | TGGCTACCAAGGTATTTGAA+TGG | - | chr8.1:25509412-25509431 | None:intergenic | 40.0% |
| ! | TTTTGGTCTCTCAAGCACAT+TGG | - | chr8.1:25509281-25509300 | None:intergenic | 40.0% |
| !! | CTTTGAAAAGTGTCATGTCC+CGG | - | chr8.1:25509456-25509475 | None:intergenic | 40.0% |
| !! | GGTGTTATTGACTTGCTTTG+AGG | - | chr8.1:25508066-25508085 | None:intergenic | 40.0% |
| !! | GTGACCTCTTTTTGTTTGGT+GGG | - | chr8.1:25508044-25508063 | None:intergenic | 40.0% |
| !! | TGACCTCTTTTTGTTTGGTG+GGG | - | chr8.1:25508043-25508062 | None:intergenic | 40.0% |
| !! | TGGAGTTGATGGAAATGAGA+AGG | - | chr8.1:25507991-25508010 | None:intergenic | 40.0% |
| !! | TTATCTTCATGGCGGATAGA+TGG | + | chr8.1:25509827-25509846 | MS.gene61138:CDS | 40.0% |
| !!! | GTTTCGAGTTTCGACTTTTC+TGG | + | chr8.1:25509759-25509778 | MS.gene61138:CDS | 40.0% |
| AACTCCTACAACCAACTGGT+AGG | - | chr8.1:25508683-25508702 | None:intergenic | 45.0% | |
| AATGGAAACTGCCTTTGGGT+TGG | + | chr8.1:25509980-25509999 | MS.gene61138:CDS | 45.0% | |
| ACCGGTCCATTCAAATACCT+TGG | + | chr8.1:25509403-25509422 | MS.gene61138:CDS | 45.0% | |
| AGACTATCTGAGAGAGCTCA+TGG | + | chr8.1:25508474-25508493 | MS.gene61138:intron | 45.0% | |
| AGGTATTTGAATGGACCGGT+AGG | - | chr8.1:25509403-25509422 | None:intergenic | 45.0% | |
| ATCACTGAATGCTATGCCGA+CGG | - | chr8.1:25509377-25509396 | None:intergenic | 45.0% | |
| CAGGTGGACATTATAGGAGT+TGG | + | chr8.1:25509163-25509182 | MS.gene61138:intron | 45.0% | |
| CCGGCTATGTTCAAACACAA+AGG | + | chr8.1:25509610-25509629 | MS.gene61138:CDS | 45.0% | |
| CTCCATCAGGTGGACATTAT+AGG | + | chr8.1:25509157-25509176 | MS.gene61138:intron | 45.0% | |
| CTCCTATAATGTCCACCTGA+TGG | - | chr8.1:25509162-25509181 | None:intergenic | 45.0% | |
| CTGAGACAATCTTAGGGACA+TGG | + | chr8.1:25509698-25509717 | MS.gene61138:CDS | 45.0% | |
| GCATTCAGTGATACTCCTAC+CGG | + | chr8.1:25509385-25509404 | MS.gene61138:CDS | 45.0% | |
| GTATCCAACCAAATTCTCCC+AGG | - | chr8.1:25508216-25508235 | None:intergenic | 45.0% | |
| TCTCACAATGTCTCTCCATC+AGG | + | chr8.1:25509144-25509163 | MS.gene61138:intron | 45.0% | |
| TGAAAGTGACTTACACGAGC+AGG | - | chr8.1:25508360-25508379 | None:intergenic | 45.0% | |
| ! | AATTTTCGTTGCAACGCTGG+AGG | + | chr8.1:25507847-25507866 | MS.gene61138:CDS | 45.0% |
| ! | ACGAATTTTCGTTGCAACGC+TGG | + | chr8.1:25507844-25507863 | MS.gene61138:CDS | 45.0% |
| ! | ATGGTTACATCAGGTTGCAC+TGG | + | chr8.1:25509643-25509662 | MS.gene61138:CDS | 45.0% |
| ! | CTTCTAAGGATCTATGGACC+TGG | + | chr8.1:25509194-25509213 | MS.gene61138:CDS | 45.0% |
| ! | GACGGAAGCTTTTGCGTAAT+TGG | - | chr8.1:25509359-25509378 | None:intergenic | 45.0% |
| ! | TACATCAGGTTGCACTGGAT+GGG | + | chr8.1:25509648-25509667 | MS.gene61138:CDS | 45.0% |
| ! | TTACATCAGGTTGCACTGGA+TGG | + | chr8.1:25509647-25509666 | MS.gene61138:CDS | 45.0% |
| !! | ACCTCTTTTTGTTTGGTGGG+GGG | - | chr8.1:25508041-25508060 | None:intergenic | 45.0% |
| !! | GACCTCTTTTTGTTTGGTGG+GGG | - | chr8.1:25508042-25508061 | None:intergenic | 45.0% |
| !! | GGTGACCTCTTTTTGTTTGG+TGG | - | chr8.1:25508045-25508064 | None:intergenic | 45.0% |
| !! | TTTTGTTTGGTGGGGGGATT+TGG | - | chr8.1:25508035-25508054 | None:intergenic | 45.0% |
| ACCGCATGGATATCAAAGCC+GGG | + | chr8.1:25509435-25509454 | MS.gene61138:CDS | 50.0% | |
| ACTGCTACCCTGGGAGAATT+TGG | + | chr8.1:25508205-25508224 | MS.gene61138:CDS | 50.0% | |
| CACAATGTCTCTCCATCAGG+TGG | + | chr8.1:25509147-25509166 | MS.gene61138:intron | 50.0% | |
| CACCCTCCATGAGCTTGAAT+AGG | - | chr8.1:25508249-25508268 | None:intergenic | 50.0% | |
| CTACCCTGGGAGAATTTGGT+TGG | + | chr8.1:25508209-25508228 | MS.gene61138:CDS | 50.0% | |
| GACCGCATGGATATCAAAGC+CGG | + | chr8.1:25509434-25509453 | MS.gene61138:CDS | 50.0% | |
| GCATCCTACCAGTTGGTTGT+AGG | + | chr8.1:25508676-25508695 | MS.gene61138:intron | 50.0% | |
| TCCCCCCACCAAACAAAAAG+AGG | + | chr8.1:25508037-25508056 | MS.gene61138:CDS | 50.0% | |
| ! | TCCCGGCTTTGATATCCATG+CGG | - | chr8.1:25509439-25509458 | None:intergenic | 50.0% |
| CATGCGGTCTTTGGCTACCA+AGG | - | chr8.1:25509423-25509442 | None:intergenic | 55.0% | |
| GGGCTAAGCATCCTACCAGT+TGG | + | chr8.1:25508669-25508688 | MS.gene61138:intron | 55.0% | |
| ! | CTTGGTAGCCAAAGACCGCA+TGG | + | chr8.1:25509421-25509440 | MS.gene61138:CDS | 55.0% |
| ! | CGGTCAGCGAAGAGAAGCGC+CGG | + | chr8.1:25509591-25509610 | MS.gene61138:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 25507811 | 25510017 | 25507811 | ID=MS.gene61138 |
| chr8.1 | mRNA | 25507811 | 25510017 | 25507811 | ID=MS.gene61138.t1;Parent=MS.gene61138 |
| chr8.1 | exon | 25507811 | 25508365 | 25507811 | ID=MS.gene61138.t1.exon1;Parent=MS.gene61138.t1 |
| chr8.1 | CDS | 25507811 | 25508365 | 25507811 | ID=cds.MS.gene61138.t1;Parent=MS.gene61138.t1 |
| chr8.1 | exon | 25509166 | 25510017 | 25509166 | ID=MS.gene61138.t1.exon2;Parent=MS.gene61138.t1 |
| chr8.1 | CDS | 25509166 | 25510017 | 25509166 | ID=cds.MS.gene61138.t1;Parent=MS.gene61138.t1 |
| Gene Sequence |
| Protein sequence |
