AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74583


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74583.t1 MTR_7g094120 85.535 159 23 0 1 159 1 159 3.87e-99 282
MS.gene74583.t1 MTR_7g021400 78.616 159 31 2 1 159 1 156 1.80e-80 235
MS.gene74583.t1 MTR_7g094280 72.500 160 41 2 1 159 1 158 2.14e-79 232
MS.gene74583.t1 MTR_7g094150 77.987 159 32 2 1 159 1 156 2.58e-79 232
MS.gene74583.t1 MTR_7g094170 76.730 159 34 2 1 159 1 156 1.11e-76 225
MS.gene74583.t1 MTR_5g022710 76.101 159 34 2 1 159 1 155 1.38e-76 225
MS.gene74583.t1 MTR_3g110048 69.811 159 47 1 1 159 1 158 2.43e-75 222
MS.gene74583.t1 MTR_5g022730 74.843 159 36 2 1 159 1 155 2.57e-75 222
MS.gene74583.t1 MTR_4g032450 68.153 157 47 2 4 159 21 175 8.77e-74 219
MS.gene74583.t1 MTR_7g021380 74.843 159 37 2 1 159 1 156 9.84e-73 215
MS.gene74583.t1 MTR_4g032400 67.925 159 48 2 2 159 7 163 1.05e-70 210
MS.gene74583.t1 MTR_7g094310 67.500 160 39 3 1 159 1 148 5.72e-70 208
MS.gene74583.t1 MTR_8g056060 66.875 160 49 3 1 159 1 157 1.98e-68 204
MS.gene74583.t1 MTR_4g032510 62.733 161 50 3 1 159 1 153 3.59e-65 196
MS.gene74583.t1 MTR_8g468480 64.375 160 52 3 1 159 1 156 2.63e-64 194
MS.gene74583.t1 MTR_4g032500 60.625 160 54 2 1 159 1 152 2.75e-64 194
MS.gene74583.t1 MTR_4g032460 63.750 160 47 3 1 159 1 150 6.12e-61 185
MS.gene74583.t1 MTR_5g085030 59.748 159 59 3 1 158 1 155 6.14e-60 183
MS.gene74583.t1 MTR_4g033455 58.599 157 62 2 3 159 7 160 3.67e-59 181
MS.gene74583.t1 MTR_4g017860 52.062 194 52 3 7 159 27 220 2.70e-56 176
MS.gene74583.t1 MTR_4g033460 58.741 143 56 2 3 145 7 146 9.65e-53 168
MS.gene74583.t1 MTR_4g033460 61.053 95 27 2 42 136 161 245 1.50e-29 108
MS.gene74583.t1 MTR_5g082340 56.250 160 54 3 1 159 1 145 1.21e-49 156
MS.gene74583.t1 MTR_4g021390 54.430 158 67 2 2 159 3 155 4.26e-49 155
MS.gene74583.t1 MTR_1g099870 74.038 104 23 2 58 159 2 103 1.92e-48 152
MS.gene74583.t1 MTR_8g089730 63.200 125 27 1 35 159 1 106 1.61e-45 145
MS.gene74583.t1 MTR_7g094320 50.314 159 37 1 1 159 1 117 1.15e-44 143
MS.gene74583.t1 MTR_4g032420 54.264 129 42 3 32 159 38 150 1.98e-41 135
MS.gene74583.t1 MTR_7g094410 52.564 156 35 2 1 117 1 156 2.38e-41 136
MS.gene74583.t1 MTR_4g032470 47.945 146 30 2 15 159 34 134 6.81e-38 126
MS.gene74583.t1 MTR_4g131980 55.072 138 49 5 1 136 1 127 1.08e-37 126
MS.gene74583.t1 MTR_7g094360 44.134 179 48 3 1 128 1 178 5.60e-37 126
MS.gene74583.t1 MTR_4g127240 61.905 105 32 4 41 143 26 124 1.77e-34 117
MS.gene74583.t1 MTR_7g006370 49.306 144 62 3 2 145 3 135 3.82e-34 117
MS.gene74583.t1 MTR_7g094350 42.632 190 54 6 1 138 1 187 1.04e-31 112
MS.gene74583.t1 MTR_8g056090 47.170 159 48 8 1 157 1 125 2.99e-29 104
MS.gene74583.t1 MTR_4g119422 40.268 149 80 2 3 149 227 368 1.49e-26 103
MS.gene74583.t1 MTR_4g032520 46.281 121 38 2 31 150 2 96 1.43e-25 95.1
MS.gene74583.t1 MTR_2g066010 38.562 153 80 4 9 158 20 161 2.87e-25 96.3
MS.gene74583.t1 MTR_2g065720 37.107 159 86 4 3 158 31 178 1.30e-24 94.7
MS.gene74583.t1 MTR_1g112590 35.256 156 81 4 11 149 3 155 6.12e-24 91.7
MS.gene74583.t1 MTR_5g096950 36.129 155 84 4 5 158 2 142 1.12e-21 85.5
MS.gene74583.t1 MTR_7g071200 59.420 69 27 1 92 159 4 72 1.34e-21 83.2
MS.gene74583.t1 MTR_2g018980 35.065 154 83 4 8 159 2 140 1.73e-21 85.1
MS.gene74583.t1 MTR_4g032540 57.143 63 26 1 93 154 70 132 1.72e-18 77.0
MS.gene74583.t1 MTR_5g078680 30.769 156 68 3 11 149 3 135 3.84e-18 79.0
MS.gene74583.t1 MTR_1g112570 34.507 142 67 4 9 131 1 135 4.37e-17 76.3
MS.gene74583.t1 MTR_1g042510 31.013 158 69 4 9 149 1 135 1.73e-16 74.7
MS.gene74583.t1 MTR_8g007185 31.293 147 86 4 11 150 13 151 5.09e-16 73.6
MS.gene74583.t1 MTR_8g007205 31.293 147 86 4 11 150 13 151 8.44e-16 72.8
MS.gene74583.t1 MTR_8g007195 33.803 142 80 4 11 146 13 146 1.01e-15 73.2
MS.gene74583.t1 MTR_6g053620 32.680 153 89 4 3 149 11 155 4.94e-15 71.2
MS.gene74583.t1 MTR_6g053620 32.680 153 89 4 3 149 11 155 6.00e-15 71.2
MS.gene74583.t1 MTR_2g100830 30.464 151 89 4 6 149 8 149 1.06e-14 70.1
MS.gene74583.t1 MTR_2g100825 30.667 150 91 3 5 149 7 148 1.94e-14 69.3
MS.gene74583.t1 MTR_8g030670 34.314 102 60 1 48 149 62 156 1.78e-13 67.0
MS.gene74583.t1 MTR_7g077810 29.801 151 90 4 6 149 15 156 3.30e-12 63.2
MS.gene74583.t1 MTR_7g036780 29.167 144 88 3 1 138 1 136 9.14e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74583.t1 AT1G20140 68.750 160 45 1 5 159 4 163 1.38e-75 223
MS.gene74583.t1 AT2G25700 66.875 160 48 1 5 159 4 163 1.63e-71 213
MS.gene74583.t1 AT1G75950 69.811 159 44 1 5 159 2 160 3.36e-71 211
MS.gene74583.t1 AT5G42190 63.529 170 48 1 4 159 2 171 1.71e-67 202
MS.gene74583.t1 AT4G34210 61.935 155 55 1 5 159 2 152 2.90e-66 199
MS.gene74583.t1 AT4G34470 60.000 155 58 1 5 159 2 152 1.72e-63 192
MS.gene74583.t1 AT3G60010 60.645 155 56 2 7 159 3 154 1.05e-60 185
MS.gene74583.t1 AT2G03190 55.556 162 61 2 9 159 6 167 1.12e-56 175
MS.gene74583.t1 AT3G21860 57.419 155 62 3 5 159 2 152 1.73e-56 174
MS.gene74583.t1 AT2G03170 56.579 152 57 2 9 159 6 149 1.15e-55 172
MS.gene74583.t1 AT3G21850 53.846 156 67 2 5 159 2 153 1.75e-53 166
MS.gene74583.t1 AT2G20160 53.548 155 64 2 5 158 2 149 7.75e-51 160
MS.gene74583.t1 AT3G25650 55.556 162 61 4 9 159 6 167 3.02e-50 159
MS.gene74583.t1 AT2G03160 48.705 193 62 5 1 159 1 190 6.39e-50 159
MS.gene74583.t1 AT3G60020 50.955 157 63 4 9 159 5 153 1.70e-48 154
MS.gene74583.t1 AT1G10230 54.305 151 68 1 9 158 6 156 5.97e-48 152
MS.gene74583.t1 AT3G21830 48.387 155 76 1 5 159 2 152 2.82e-45 145
MS.gene74583.t1 AT3G21840 49.194 124 59 3 5 128 2 121 1.82e-30 107
MS.gene74583.t1 AT3G53060 58.140 86 27 3 32 116 3 80 6.02e-25 92.0
MS.gene74583.t1 AT2G45950 31.788 151 91 4 4 149 13 156 2.90e-15 72.0
MS.gene74583.t1 AT2G45950 31.788 151 91 4 4 149 13 156 2.90e-15 72.0
MS.gene74583.t1 AT2G45950 31.788 151 91 4 4 149 13 156 3.75e-15 71.6
MS.gene74583.t1 AT2G45950 31.788 151 91 4 4 149 13 156 3.75e-15 71.6
MS.gene74583.t1 AT2G45950 31.788 151 91 4 4 149 13 156 3.75e-15 71.6
MS.gene74583.t1 AT3G61415 31.333 150 89 4 6 149 15 156 9.07e-14 67.8
MS.gene74583.t1 AT3G61415 31.333 150 89 4 6 149 15 156 1.12e-13 67.4

Find 41 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCAGGGTGATCGTCTTCT+TGG 0.232979 2.3:+60336549 None:intergenic
CAGAAGATTATCTTAAGAAT+TGG 0.268289 2.3:-60336330 MS.gene74583:CDS
TTTCACCGTCGAAACTCTTC+AGG 0.292899 2.3:+60336533 None:intergenic
TACCTTCGCTAATACCTTGC+CGG 0.357004 2.3:+60336411 None:intergenic
TGCCGGTAACGTTCTGAATC+GGG 0.375308 2.3:+60336428 None:intergenic
CATGTCCGCCGCAGCTTGGC+AGG 0.378684 2.3:+60336216 None:intergenic
CTGAATCGGGATTGCGTTGT+CGG 0.392692 2.3:+60336441 None:intergenic
AGAAGATTATCTTAAGAATT+GGG 0.396941 2.3:-60336329 MS.gene74583:CDS
ATCCCGATTCAGAACGTTAC+CGG 0.408425 2.3:-60336430 MS.gene74583:CDS
CATCCTTGCTGCAAACTACT+TGG 0.414458 2.3:-60336263 MS.gene74583:CDS
CGAACTTCTTCCTCTTCCTC+CGG 0.424372 2.3:+60336133 None:intergenic
TTGCCGGTAACGTTCTGAAT+CGG 0.445008 2.3:+60336427 None:intergenic
CTTGGATATCAAGAGTCTAC+TGG 0.463162 2.3:-60336245 MS.gene74583:CDS
TCATCATGTCCGCCGCAGCT+TGG 0.472634 2.3:+60336212 None:intergenic
TGATTTCGTGAAGGTTGATA+AGG 0.476483 2.3:-60336302 MS.gene74583:CDS
TGATTACACTCCGGAGGAAG+AGG 0.478345 2.3:-60336143 MS.gene74583:CDS
ATTCGAGGTTTCCGAGGCTG+TGG 0.512799 2.3:-60336509 MS.gene74583:CDS
CTTCTTGGTTGTTGAAGACA+TGG 0.520126 2.3:+60336564 None:intergenic
TGATTCAAGCGCCACAGCCT+CGG 0.536432 2.3:+60336498 None:intergenic
AGATAATCTTCTGATTCTGA+AGG 0.536670 2.3:+60336340 None:intergenic
TTCACCGTCGAAACTCTTCA+GGG 0.545848 2.3:+60336534 None:intergenic
TCTTACCTGCCAAGCTGCGG+CGG 0.548433 2.3:-60336221 MS.gene74583:CDS
CATGATGAAAGGAAAGAAAG+TGG 0.561795 2.3:-60336197 MS.gene74583:CDS
GGAAGAGGAAGAAGTTCGAA+AGG 0.564146 2.3:-60336128 MS.gene74583:CDS
TTTCGACGGTGAAACATTCG+AGG 0.567957 2.3:-60336524 MS.gene74583:CDS
AAGTTCGAAAGGAAAACGCA+TGG 0.586714 2.3:-60336117 MS.gene74583:CDS
GATTCAGAACGTTACCGGCA+AGG 0.604913 2.3:-60336425 MS.gene74583:CDS
ATCACCCTGAAGAGTTTCGA+CGG 0.611256 2.3:-60336538 MS.gene74583:CDS
GACAATCAAGCACATGATCG+AGG 0.614315 2.3:-60336473 MS.gene74583:CDS
TGAAACATTCGAGGTTTCCG+AGG 0.617609 2.3:-60336515 MS.gene74583:CDS
GCTGCGGCGGACATGATGAA+AGG 0.621251 2.3:-60336208 MS.gene74583:CDS
TACCGGCAAGGTATTAGCGA+AGG 0.638623 2.3:-60336413 MS.gene74583:CDS
AGTTCGAAAGGAAAACGCAT+GGG 0.640914 2.3:-60336116 MS.gene74583:CDS
GATGAAAGGAAAGAAAGTGG+AGG 0.641593 2.3:-60336194 MS.gene74583:CDS
CTGCAAGAAGCACGTCGAGG+TGG 0.646155 2.3:-60336380 MS.gene74583:CDS
TATCCAAGTAGTTTGCAGCA+AGG 0.660134 2.3:+60336260 None:intergenic
GGATCTTACCTGCCAAGCTG+CGG 0.660372 2.3:-60336224 MS.gene74583:CDS
TTGGGATGCTGATTTCGTGA+AGG 0.669657 2.3:-60336311 MS.gene74583:CDS
CAAGAATGATTACACTCCGG+AGG 0.672447 2.3:-60336149 MS.gene74583:CDS
CATCAAGAATGATTACACTC+CGG 0.688170 2.3:-60336152 MS.gene74583:CDS
ATACTGCAAGAAGCACGTCG+AGG 0.756219 2.3:-60336383 MS.gene74583:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAGATTATCTTAAGAATT+GGG - chr2.3:60336338-60336357 MS.gene74583:CDS 20.0%
! CAGAAGATTATCTTAAGAAT+TGG - chr2.3:60336337-60336356 MS.gene74583:CDS 25.0%
AGATAATCTTCTGATTCTGA+AGG + chr2.3:60336330-60336349 None:intergenic 30.0%
!! TGATTCTGAAGGTTTTTCAT+CGG + chr2.3:60336319-60336338 None:intergenic 30.0%
CATCAAGAATGATTACACTC+CGG - chr2.3:60336515-60336534 MS.gene74583:CDS 35.0%
CATGATGAAAGGAAAGAAAG+TGG - chr2.3:60336470-60336489 MS.gene74583:CDS 35.0%
!! TGATTTCGTGAAGGTTGATA+AGG - chr2.3:60336365-60336384 MS.gene74583:CDS 35.0%
AAGTTCGAAAGGAAAACGCA+TGG - chr2.3:60336550-60336569 MS.gene74583:CDS 40.0%
AGTTCGAAAGGAAAACGCAT+GGG - chr2.3:60336551-60336570 MS.gene74583:CDS 40.0%
CTTGGATATCAAGAGTCTAC+TGG - chr2.3:60336422-60336441 MS.gene74583:CDS 40.0%
GATGAAAGGAAAGAAAGTGG+AGG - chr2.3:60336473-60336492 MS.gene74583:CDS 40.0%
GCAAGGATGAGTTCAAAAAG+TGG + chr2.3:60336393-60336412 None:intergenic 40.0%
! TATCCAAGTAGTTTGCAGCA+AGG + chr2.3:60336410-60336429 None:intergenic 40.0%
ATCACCCTGAAGAGTTTCGA+CGG - chr2.3:60336129-60336148 MS.gene74583:CDS 45.0%
ATCCCGATTCAGAACGTTAC+CGG - chr2.3:60336237-60336256 MS.gene74583:CDS 45.0%
CAAGAATGATTACACTCCGG+AGG - chr2.3:60336518-60336537 MS.gene74583:CDS 45.0%
CATCCTTGCTGCAAACTACT+TGG - chr2.3:60336404-60336423 MS.gene74583:CDS 45.0%
GACAATCAAGCACATGATCG+AGG - chr2.3:60336194-60336213 MS.gene74583:CDS 45.0%
GGAAGAGGAAGAAGTTCGAA+AGG - chr2.3:60336539-60336558 MS.gene74583:CDS 45.0%
TACCTTCGCTAATACCTTGC+CGG + chr2.3:60336259-60336278 None:intergenic 45.0%
TGAAACATTCGAGGTTTCCG+AGG - chr2.3:60336152-60336171 MS.gene74583:CDS 45.0%
TTCACCGTCGAAACTCTTCA+GGG + chr2.3:60336136-60336155 None:intergenic 45.0%
TTTCACCGTCGAAACTCTTC+AGG + chr2.3:60336137-60336156 None:intergenic 45.0%
TTTCGACGGTGAAACATTCG+AGG - chr2.3:60336143-60336162 MS.gene74583:CDS 45.0%
! TTGCCGGTAACGTTCTGAAT+CGG + chr2.3:60336243-60336262 None:intergenic 45.0%
!! TTGGGATGCTGATTTCGTGA+AGG - chr2.3:60336356-60336375 MS.gene74583:CDS 45.0%
CGAACTTCTTCCTCTTCCTC+CGG + chr2.3:60336537-60336556 None:intergenic 50.0%
CTGAATCGGGATTGCGTTGT+CGG + chr2.3:60336229-60336248 None:intergenic 50.0%
CTTCAGGGTGATCGTCTTCT+TGG + chr2.3:60336121-60336140 None:intergenic 50.0%
GATTCAGAACGTTACCGGCA+AGG - chr2.3:60336242-60336261 MS.gene74583:CDS 50.0%
TGATTACACTCCGGAGGAAG+AGG - chr2.3:60336524-60336543 MS.gene74583:CDS 50.0%
! ATACTGCAAGAAGCACGTCG+AGG - chr2.3:60336284-60336303 MS.gene74583:CDS 50.0%
! TACCGGCAAGGTATTAGCGA+AGG - chr2.3:60336254-60336273 MS.gene74583:CDS 50.0%
! TGCCGGTAACGTTCTGAATC+GGG + chr2.3:60336242-60336261 None:intergenic 50.0%
ATTCGAGGTTTCCGAGGCTG+TGG - chr2.3:60336158-60336177 MS.gene74583:CDS 55.0%
GGATCTTACCTGCCAAGCTG+CGG - chr2.3:60336443-60336462 MS.gene74583:CDS 55.0%
TGATTCAAGCGCCACAGCCT+CGG + chr2.3:60336172-60336191 None:intergenic 55.0%
GCTGCGGCGGACATGATGAA+AGG - chr2.3:60336459-60336478 MS.gene74583:CDS 60.0%
TCATCATGTCCGCCGCAGCT+TGG + chr2.3:60336458-60336477 None:intergenic 60.0%
TCTTACCTGCCAAGCTGCGG+CGG - chr2.3:60336446-60336465 MS.gene74583:CDS 60.0%
! CTGCAAGAAGCACGTCGAGG+TGG - chr2.3:60336287-60336306 MS.gene74583:CDS 60.0%
CATGTCCGCCGCAGCTTGGC+AGG + chr2.3:60336454-60336473 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 60336105 60336584 60336105 ID=MS.gene74583
chr2.3 mRNA 60336105 60336584 60336105 ID=MS.gene74583.t1;Parent=MS.gene74583
chr2.3 exon 60336105 60336584 60336105 ID=MS.gene74583.t1.exon1;Parent=MS.gene74583.t1
chr2.3 CDS 60336105 60336584 60336105 ID=cds.MS.gene74583.t1;Parent=MS.gene74583.t1
Gene Sequence

>MS.gene74583

ATGTCTTCAACAACCAAGAAGACGATCACCCTGAAGAGTTTCGACGGTGAAACATTCGAGGTTTCCGAGGCTGTGGCGCTTGAATCACAGACAATCAAGCACATGATCGAGGATGATTGTGCCGACAACGCAATCCCGATTCAGAACGTTACCGGCAAGGTATTAGCGAAGGTAATTGAATACTGCAAGAAGCACGTCGAGGTGGCGAGTGCCGATGAAAAACCTTCAGAATCAGAAGATTATCTTAAGAATTGGGATGCTGATTTCGTGAAGGTTGATAAGGAACCACTTTTTGAACTCATCCTTGCTGCAAACTACTTGGATATCAAGAGTCTACTGGATCTTACCTGCCAAGCTGCGGCGGACATGATGAAAGGAAAGAAAGTGGAGGAAGTTCGCACGATGTTTAACATCAAGAATGATTACACTCCGGAGGAAGAGGAAGAAGTTCGAAAGGAAAACGCATGGGCATTTGAATGA

Protein sequence

>MS.gene74583.t1

MSSTTKKTITLKSFDGETFEVSEAVALESQTIKHMIEDDCADNAIPIQNVTGKVLAKVIEYCKKHVEVASADEKPSESEDYLKNWDADFVKVDKEPLFELILAANYLDIKSLLDLTCQAAADMMKGKKVEEVRTMFNIKNDYTPEEEEEVRKENAWAFE