Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80098.t1 | XP_003618753.2 | 89.3 | 112 | 5 | 3 | 1 | 112 | 1 | 105 | 1.50E-41 | 178.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80098.t1 | G7KMT1 | 89.3 | 112 | 5 | 3 | 1 | 112 | 1 | 105 | 1.1e-41 | 178.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene80098 | 0.827305 | 1.68E-54 | -1.69E-46 |
| MS.gene049217 | MS.gene80098 | 0.819875 | 9.23E-53 | -1.69E-46 |
| MS.gene049222 | MS.gene80098 | 0.822435 | 2.37E-53 | -1.69E-46 |
| MS.gene049232 | MS.gene80098 | 0.808419 | 3.13E-50 | -1.69E-46 |
| MS.gene049285 | MS.gene80098 | 0.832953 | 7.04E-56 | -1.69E-46 |
| MS.gene049320 | MS.gene80098 | 0.81397 | 1.95E-51 | -1.69E-46 |
| MS.gene049427 | MS.gene80098 | 0.81286 | 3.43E-51 | -1.69E-46 |
| MS.gene049609 | MS.gene80098 | 0.810399 | 1.18E-50 | -1.69E-46 |
| MS.gene049627 | MS.gene80098 | 0.840009 | 1.13E-57 | -1.69E-46 |
| MS.gene049633 | MS.gene80098 | 0.822066 | 2.89E-53 | -1.69E-46 |
| MS.gene049805 | MS.gene80098 | 0.820802 | 5.66E-53 | -1.69E-46 |
| MS.gene050163 | MS.gene80098 | 0.824466 | 7.95E-54 | -1.69E-46 |
| MS.gene050165 | MS.gene80098 | 0.802986 | 4.32E-49 | -1.69E-46 |
| MS.gene050333 | MS.gene80098 | 0.814489 | 1.50E-51 | -1.69E-46 |
| MS.gene050504 | MS.gene80098 | 0.82257 | 2.21E-53 | -1.69E-46 |
| MS.gene050518 | MS.gene80098 | 0.814183 | 1.75E-51 | -1.69E-46 |
| MS.gene050577 | MS.gene80098 | 0.843295 | 1.53E-58 | -1.69E-46 |
| MS.gene050699 | MS.gene80098 | 0.817739 | 2.82E-52 | -1.69E-46 |
| MS.gene050747 | MS.gene80098 | 0.847201 | 1.34E-59 | -1.69E-46 |
| MS.gene050772 | MS.gene80098 | 0.836673 | 8.15E-57 | -1.69E-46 |
| MS.gene050950 | MS.gene80098 | 0.844799 | 6.04E-59 | -1.69E-46 |
| MS.gene05100 | MS.gene80098 | 0.807082 | 6.01E-50 | -1.69E-46 |
| MS.gene051694 | MS.gene80098 | 0.816194 | 6.27E-52 | -1.69E-46 |
| MS.gene051858 | MS.gene80098 | 0.805232 | 1.47E-49 | -1.69E-46 |
| MS.gene052482 | MS.gene80098 | 0.81827 | 2.14E-52 | -1.69E-46 |
| MS.gene052486 | MS.gene80098 | 0.839086 | 1.96E-57 | -1.69E-46 |
| MS.gene052503 | MS.gene80098 | 0.810036 | 1.41E-50 | -1.69E-46 |
| MS.gene052615 | MS.gene80098 | 0.832863 | 7.42E-56 | -1.69E-46 |
| MS.gene052823 | MS.gene80098 | 0.806676 | 7.33E-50 | -1.69E-46 |
| MS.gene052825 | MS.gene80098 | 0.803806 | 2.92E-49 | -1.69E-46 |
| MS.gene053203 | MS.gene80098 | 0.819581 | 1.08E-52 | -1.69E-46 |
| MS.gene053213 | MS.gene80098 | 0.84243 | 2.60E-58 | -1.69E-46 |
| MS.gene053286 | MS.gene80098 | 0.837455 | 5.15E-57 | -1.69E-46 |
| MS.gene053436 | MS.gene80098 | 0.821524 | 3.86E-53 | -1.69E-46 |
| MS.gene053480 | MS.gene80098 | 0.802087 | 6.62E-49 | -1.69E-46 |
| MS.gene053574 | MS.gene80098 | 0.824431 | 8.10E-54 | -1.69E-46 |
| MS.gene053626 | MS.gene80098 | 0.816232 | 6.15E-52 | -1.69E-46 |
| MS.gene054408 | MS.gene80098 | 0.83557 | 1.55E-56 | -1.69E-46 |
| MS.gene054595 | MS.gene80098 | 0.842841 | 2.02E-58 | -1.69E-46 |
| MS.gene054642 | MS.gene80098 | 0.813646 | 2.30E-51 | -1.69E-46 |
| MS.gene05470 | MS.gene80098 | 0.804829 | 1.79E-49 | -1.69E-46 |
| MS.gene054954 | MS.gene80098 | 0.815269 | 1.01E-51 | -1.69E-46 |
| MS.gene055206 | MS.gene80098 | 0.800673 | 1.29E-48 | -1.69E-46 |
| MS.gene055362 | MS.gene80098 | 0.824413 | 8.18E-54 | -1.69E-46 |
| MS.gene055502 | MS.gene80098 | 0.835016 | 2.14E-56 | -1.69E-46 |
| MS.gene055647 | MS.gene80098 | 0.82509 | 5.66E-54 | -1.69E-46 |
| MS.gene055722 | MS.gene80098 | 0.815192 | 1.05E-51 | -1.69E-46 |
| MS.gene056040 | MS.gene80098 | 0.810181 | 1.31E-50 | -1.69E-46 |
| MS.gene056166 | MS.gene80098 | 0.818021 | 2.44E-52 | -1.69E-46 |
| MS.gene056374 | MS.gene80098 | 0.80984 | 1.55E-50 | -1.69E-46 |
| MS.gene056504 | MS.gene80098 | 0.813662 | 2.29E-51 | -1.69E-46 |
| MS.gene056510 | MS.gene80098 | 0.824693 | 7.03E-54 | -1.69E-46 |
| MS.gene056549 | MS.gene80098 | 0.846325 | 2.33E-59 | -1.69E-46 |
| MS.gene056657 | MS.gene80098 | 0.825564 | 4.38E-54 | -1.69E-46 |
| MS.gene056692 | MS.gene80098 | 0.820163 | 7.93E-53 | -1.69E-46 |
| MS.gene057146 | MS.gene80098 | 0.816067 | 6.69E-52 | -1.69E-46 |
| MS.gene057416 | MS.gene80098 | 0.836959 | 6.89E-57 | -1.69E-46 |
| MS.gene057831 | MS.gene80098 | 0.849399 | 3.32E-60 | -1.69E-46 |
| MS.gene058019 | MS.gene80098 | 0.801517 | 8.67E-49 | -1.69E-46 |
| MS.gene05837 | MS.gene80098 | 0.8175 | 3.19E-52 | -1.69E-46 |
| MS.gene058419 | MS.gene80098 | 0.841864 | 3.67E-58 | -1.69E-46 |
| MS.gene058544 | MS.gene80098 | 0.834157 | 3.53E-56 | -1.69E-46 |
| MS.gene05883 | MS.gene80098 | 0.860059 | 2.69E-63 | -1.69E-46 |
| MS.gene059022 | MS.gene80098 | 0.80761 | 4.65E-50 | -1.69E-46 |
| MS.gene059023 | MS.gene80098 | 0.810711 | 1.01E-50 | -1.69E-46 |
| MS.gene05934 | MS.gene80098 | 0.805918 | 1.06E-49 | -1.69E-46 |
| MS.gene059536 | MS.gene80098 | 0.819843 | 9.39E-53 | -1.69E-46 |
| MS.gene059677 | MS.gene80098 | 0.809027 | 2.32E-50 | -1.69E-46 |
| MS.gene059718 | MS.gene80098 | 0.838928 | 2.15E-57 | -1.69E-46 |
| MS.gene059756 | MS.gene80098 | 0.839873 | 1.22E-57 | -1.69E-46 |
| MS.gene059801 | MS.gene80098 | 0.829998 | 3.76E-55 | -1.69E-46 |
| MS.gene060482 | MS.gene80098 | 0.802329 | 5.91E-49 | -1.69E-46 |
| MS.gene060762 | MS.gene80098 | 0.812206 | 4.77E-51 | -1.69E-46 |
| MS.gene06077 | MS.gene80098 | 0.839868 | 1.22E-57 | -1.69E-46 |
| MS.gene06092 | MS.gene80098 | 0.803959 | 2.72E-49 | -1.69E-46 |
| MS.gene060982 | MS.gene80098 | 0.810428 | 1.16E-50 | -1.69E-46 |
| MS.gene061134 | MS.gene80098 | 0.808087 | 3.68E-50 | -1.69E-46 |
| MS.gene061150 | MS.gene80098 | 0.820937 | 5.27E-53 | -1.69E-46 |
| MS.gene061171 | MS.gene80098 | 0.832873 | 7.37E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80098.t1 | MTR_6g021510 | 80.357 | 112 | 15 | 3 | 1 | 112 | 1 | 105 | 9.28e-46 | 147 |
| MS.gene80098.t1 | MTR_6g021510 | 80.357 | 112 | 15 | 3 | 1 | 112 | 1 | 105 | 2.09e-45 | 148 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80098.t1 | AT1G26180 | 45.556 | 90 | 36 | 4 | 34 | 112 | 29 | 116 | 2.47e-15 | 68.9 |
| MS.gene80098.t1 | AT1G26180 | 50.685 | 73 | 24 | 3 | 50 | 112 | 46 | 116 | 6.59e-15 | 68.9 |
Find 31 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCATGTTTGCTGCTCTTAC+TGG | 0.140429 | 6.1:-4997457 | MS.gene80098:CDS |
| TTCTGATACACTTCCCCTTC+TGG | 0.257259 | 6.1:+4997502 | None:intergenic |
| CAACAATGATGAAGAAGTTA+AGG | 0.331621 | 6.1:-4997533 | MS.gene80098:CDS |
| GAGAAAGTGGGAAGTGGGTT+TGG | 0.355078 | 6.1:+4997673 | None:intergenic |
| AAGTGGGTTTGGTTTGGAAA+TGG | 0.378361 | 6.1:+4997684 | None:intergenic |
| AGTGGGAAGTGGGTTTGGTT+TGG | 0.383262 | 6.1:+4997678 | None:intergenic |
| GAGTAATGCTCTTGCTATTG+AGG | 0.388464 | 6.1:-4997419 | None:intergenic |
| CCATGTTTGCTGCTCTTACT+GGG | 0.408995 | 6.1:-4997456 | MS.gene80098:CDS |
| CACGTTCATTCTCTCTTCAA+AGG | 0.430651 | 6.1:-4997582 | MS.gene80098:CDS |
| TGGAGAGAGAAAGTGGGAAG+TGG | 0.431283 | 6.1:+4997667 | None:intergenic |
| GGAGAGAGAAAGTGGGAAGT+GGG | 0.447148 | 6.1:+4997668 | None:intergenic |
| AGTTTGAGAGGAAGTTTCAA+TGG | 0.456612 | 6.1:+4997610 | None:intergenic |
| GTAAGAGTGGAGAGAGAAAG+TGG | 0.456947 | 6.1:+4997660 | None:intergenic |
| GTTTGCTGCTCTTACTGGGT+TGG | 0.463808 | 6.1:-4997452 | MS.gene80098:CDS |
| AGGAGAAGATGAAGGGAGAA+TGG | 0.470771 | 6.1:+4997707 | None:intergenic |
| ACGTTCATTCTCTCTTCAAA+GGG | 0.480590 | 6.1:-4997581 | MS.gene80098:CDS |
| TGGGTTTGGTTTGGAAATGG+AGG | 0.489626 | 6.1:+4997687 | None:intergenic |
| CCCAGTAAGAGCAGCAAACA+TGG | 0.492125 | 6.1:+4997456 | None:intergenic |
| GGAAATGGAGGAGAAGATGA+AGG | 0.492474 | 6.1:+4997699 | None:intergenic |
| GAAGAGAGAGAAAGTAAGAG+TGG | 0.523518 | 6.1:+4997647 | None:intergenic |
| TAAGAGTGGAGAGAGAAAGT+GGG | 0.524810 | 6.1:+4997661 | None:intergenic |
| GAACGTGGTTTGAGTTTGAG+AGG | 0.526236 | 6.1:+4997598 | None:intergenic |
| TAAGGATTTGTCACCAGAAG+GGG | 0.547246 | 6.1:-4997515 | MS.gene80098:CDS |
| AGTTTCAATGGTGGTGATGA+TGG | 0.561851 | 6.1:+4997622 | None:intergenic |
| GTTAAGGATTTGTCACCAGA+AGG | 0.563643 | 6.1:-4997517 | MS.gene80098:CDS |
| TTGAGAGGAAGTTTCAATGG+TGG | 0.574319 | 6.1:+4997613 | None:intergenic |
| GAAATGGAGGAGAAGATGAA+GGG | 0.581880 | 6.1:+4997700 | None:intergenic |
| TTAAGGATTTGTCACCAGAA+GGG | 0.582153 | 6.1:-4997516 | MS.gene80098:CDS |
| CTTTGAAGAGAGAATGAACG+TGG | 0.604224 | 6.1:+4997583 | None:intergenic |
| GTATCAGAAGACACTTCAGT+TGG | 0.638228 | 6.1:-4997488 | MS.gene80098:CDS |
| TTCAATGGTGGTGATGATGG+CGG | 0.713813 | 6.1:+4997625 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CAACAATGATGAAGAAGTTA+AGG | - | chr6.1:4997620-4997639 | MS.gene80098:CDS | 30.0% | |
| ACGTTCATTCTCTCTTCAAA+GGG | - | chr6.1:4997572-4997591 | MS.gene80098:CDS | 35.0% | |
| AGTTTGAGAGGAAGTTTCAA+TGG | + | chr6.1:4997546-4997565 | None:intergenic | 35.0% | |
| TTAAGGATTTGTCACCAGAA+GGG | - | chr6.1:4997637-4997656 | MS.gene80098:CDS | 35.0% | |
| !!! | TGGTGTTGATAAGTTTTCAG+TGG | + | chr6.1:4997429-4997448 | None:intergenic | 35.0% |
| CACGTTCATTCTCTCTTCAA+AGG | - | chr6.1:4997571-4997590 | MS.gene80098:CDS | 40.0% | |
| GAAATGGAGGAGAAGATGAA+GGG | + | chr6.1:4997456-4997475 | None:intergenic | 40.0% | |
| GAAGAGAGAGAAAGTAAGAG+TGG | + | chr6.1:4997509-4997528 | None:intergenic | 40.0% | |
| GTATCAGAAGACACTTCAGT+TGG | - | chr6.1:4997665-4997684 | MS.gene80098:CDS | 40.0% | |
| GTTAAGGATTTGTCACCAGA+AGG | - | chr6.1:4997636-4997655 | MS.gene80098:CDS | 40.0% | |
| TAAGAGTGGAGAGAGAAAGT+GGG | + | chr6.1:4997495-4997514 | None:intergenic | 40.0% | |
| TAAGGATTTGTCACCAGAAG+GGG | - | chr6.1:4997638-4997657 | MS.gene80098:CDS | 40.0% | |
| TTGAGAGGAAGTTTCAATGG+TGG | + | chr6.1:4997543-4997562 | None:intergenic | 40.0% | |
| ! | AGTTTCAATGGTGGTGATGA+TGG | + | chr6.1:4997534-4997553 | None:intergenic | 40.0% |
| ! | CTTTGAAGAGAGAATGAACG+TGG | + | chr6.1:4997573-4997592 | None:intergenic | 40.0% |
| !! | AAGTGGGTTTGGTTTGGAAA+TGG | + | chr6.1:4997472-4997491 | None:intergenic | 40.0% |
| AGGAGAAGATGAAGGGAGAA+TGG | + | chr6.1:4997449-4997468 | None:intergenic | 45.0% | |
| GGAAATGGAGGAGAAGATGA+AGG | + | chr6.1:4997457-4997476 | None:intergenic | 45.0% | |
| GTAAGAGTGGAGAGAGAAAG+TGG | + | chr6.1:4997496-4997515 | None:intergenic | 45.0% | |
| TTCTGATACACTTCCCCTTC+TGG | + | chr6.1:4997654-4997673 | None:intergenic | 45.0% | |
| ! | CCATGTTTGCTGCTCTTACT+GGG | - | chr6.1:4997697-4997716 | MS.gene80098:CDS | 45.0% |
| ! | GAACGTGGTTTGAGTTTGAG+AGG | + | chr6.1:4997558-4997577 | None:intergenic | 45.0% |
| ! | TCCATGTTTGCTGCTCTTAC+TGG | - | chr6.1:4997696-4997715 | MS.gene80098:CDS | 45.0% |
| !! | TGGGTTTGGTTTGGAAATGG+AGG | + | chr6.1:4997469-4997488 | None:intergenic | 45.0% |
| !! | TTCAATGGTGGTGATGATGG+CGG | + | chr6.1:4997531-4997550 | None:intergenic | 45.0% |
| CCCAGTAAGAGCAGCAAACA+TGG | + | chr6.1:4997700-4997719 | None:intergenic | 50.0% | |
| GGAGAGAGAAAGTGGGAAGT+GGG | + | chr6.1:4997488-4997507 | None:intergenic | 50.0% | |
| TGGAGAGAGAAAGTGGGAAG+TGG | + | chr6.1:4997489-4997508 | None:intergenic | 50.0% | |
| ! | AGTGGGAAGTGGGTTTGGTT+TGG | + | chr6.1:4997478-4997497 | None:intergenic | 50.0% |
| ! | GAGAAAGTGGGAAGTGGGTT+TGG | + | chr6.1:4997483-4997502 | None:intergenic | 50.0% |
| ! | GTTTGCTGCTCTTACTGGGT+TGG | - | chr6.1:4997701-4997720 | MS.gene80098:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 4997420 | 4997755 | 4997420 | ID=MS.gene80098 |
| chr6.1 | mRNA | 4997420 | 4997755 | 4997420 | ID=MS.gene80098.t1;Parent=MS.gene80098 |
| chr6.1 | exon | 4997420 | 4997755 | 4997420 | ID=MS.gene80098.t1.exon1;Parent=MS.gene80098.t1 |
| chr6.1 | CDS | 4997420 | 4997755 | 4997420 | ID=cds.MS.gene80098.t1;Parent=MS.gene80098.t1 |
| Gene Sequence |
| Protein sequence |
