AlfalfaGEDB | MS.gene81090

M. sativa cultivar XinJiangDaYe / MS.gene81090

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81090.t1	XP_013461904.1	94.5	164	9	0	1	164	83	246	2.10E-82	315.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81090.t1	Q9FLA1	31.0	113	73	2	4	111	76	188	1.5e-07	57.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81090.t1	A0A072V1Q5	94.5	164	9	0	1	164	83	246	1.5e-82	315.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene36942	MS.gene81090	PPI
MS.gene81090	MS.gene033695	PPI
MS.gene045772	MS.gene81090	PPI
MS.gene81090	MS.gene51795	PPI
MS.gene36763	MS.gene81090	PPI
MS.gene006730	MS.gene81090	PPI
MS.gene36804	MS.gene81090	PPI
MS.gene69561	MS.gene81090	PPI
MS.gene047136	MS.gene81090	PPI
MS.gene91886	MS.gene81090	PPI
MS.gene045448	MS.gene81090	PPI
MS.gene032852	MS.gene81090	PPI
MS.gene069762	MS.gene81090	PPI
MS.gene81090	MS.gene003455	PPI
MS.gene072000	MS.gene81090	PPI
MS.gene81090	MS.gene072231	PPI
MS.gene81090	MS.gene031577	PPI
MS.gene032379	MS.gene81090	PPI
MS.gene81090	MS.gene069763	PPI
MS.gene78164	MS.gene81090	PPI
MS.gene045440	MS.gene81090	PPI
MS.gene032862	MS.gene81090	PPI
MS.gene51793	MS.gene81090	PPI
MS.gene006736	MS.gene81090	PPI
MS.gene96357	MS.gene81090	PPI
MS.gene54960	MS.gene81090	PPI
MS.gene033694	MS.gene81090	PPI
MS.gene94458	MS.gene81090	PPI
MS.gene36809	MS.gene81090	PPI
MS.gene045437	MS.gene81090	PPI
MS.gene95902	MS.gene81090	PPI
MS.gene73780	MS.gene81090	PPI
MS.gene81090	MS.gene031575	PPI
MS.gene021295	MS.gene81090	PPI
MS.gene36807	MS.gene81090	PPI
MS.gene23706	MS.gene81090	PPI
MS.gene072003	MS.gene81090	PPI
MS.gene81090	MS.gene045449	PPI
MS.gene36743	MS.gene81090	PPI
MS.gene007794	MS.gene81090	PPI
MS.gene045451	MS.gene81090	PPI
MS.gene065373	MS.gene81090	PPI
MS.gene041323	MS.gene81090	PPI
MS.gene072244	MS.gene81090	PPI
MS.gene002112	MS.gene81090	PPI
MS.gene031570	MS.gene81090	PPI
MS.gene81090	MS.gene36943	PPI
MS.gene031603	MS.gene81090	PPI
MS.gene071257	MS.gene81090	PPI
MS.gene032861	MS.gene81090	PPI
MS.gene028696	MS.gene81090	PPI
MS.gene32305	MS.gene81090	PPI
MS.gene031573	MS.gene81090	PPI
MS.gene81090	MS.gene024262	PPI
MS.gene006727	MS.gene81090	PPI
MS.gene006728	MS.gene81090	PPI
MS.gene006725	MS.gene81090	PPI
MS.gene29873	MS.gene81090	PPI
MS.gene36810	MS.gene81090	PPI
MS.gene94460	MS.gene81090	PPI
MS.gene73831	MS.gene81090	PPI
MS.gene81090	MS.gene032860	PPI
MS.gene041325	MS.gene81090	PPI
MS.gene81090	MS.gene006723	PPI
MS.gene047137	MS.gene81090	PPI
MS.gene54959	MS.gene81090	PPI
MS.gene81090	MS.gene52136	PPI
MS.gene94457	MS.gene81090	PPI
MS.gene045439	MS.gene81090	PPI
MS.gene035630	MS.gene81090	PPI
MS.gene041326	MS.gene81090	PPI
MS.gene81090	MS.gene074346	PPI
MS.gene055754	MS.gene81090	PPI
MS.gene045446	MS.gene81090	PPI
MS.gene032853	MS.gene81090	PPI
MS.gene36752	MS.gene81090	PPI
MS.gene91884	MS.gene81090	PPI
MS.gene32300	MS.gene81090	PPI
MS.gene031572	MS.gene81090	PPI
MS.gene003456	MS.gene81090	PPI
MS.gene045452	MS.gene81090	PPI
MS.gene045441	MS.gene81090	PPI
MS.gene91885	MS.gene81090	PPI
MS.gene006692	MS.gene81090	PPI
MS.gene01653	MS.gene81090	PPI
MS.gene96120	MS.gene81090	PPI
MS.gene36764	MS.gene81090	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81090.t1	MTR_3g108560	94.512	164	9	0	1	164	83	246	4.11e-112	322
MS.gene81090.t1	MTR_3g108560	94.512	164	9	0	1	164	83	246	5.43e-111	322
MS.gene81090.t1	MTR_3g108150	48.795	166	77	4	1	162	76	237	3.70e-38	135
MS.gene81090.t1	MTR_7g088260	50.595	168	67	7	1	161	154	312	1.57e-37	134
MS.gene81090.t1	MTR_0492s0020	51.497	167	66	7	1	162	149	305	2.81e-37	133
MS.gene81090.t1	MTR_0543s0010	51.497	167	66	7	1	162	149	305	5.03e-37	133
MS.gene81090.t1	MTR_3g087260	51.515	132	57	3	34	162	184	311	2.78e-31	117
MS.gene81090.t1	MTR_3g087260	54.902	102	42	3	19	117	81	181	3.00e-23	95.9
MS.gene81090.t1	MTR_7g056020	44.706	170	79	5	1	166	72	230	5.38e-29	112
MS.gene81090.t1	MTR_3g109982	61.290	93	35	1	51	143	222	313	3.38e-27	107
MS.gene81090.t1	MTR_3g109982	51.261	119	52	3	1	114	76	193	3.21e-25	102
MS.gene81090.t1	MTR_3g110010	61.290	93	35	1	51	143	222	313	3.82e-27	107
MS.gene81090.t1	MTR_3g110010	51.261	119	52	3	1	114	76	193	4.23e-25	101
MS.gene81090.t1	MTR_5g073420	35.762	151	75	4	4	143	82	221	8.54e-18	80.1
MS.gene81090.t1	MTR_5g061680	40.741	108	59	1	8	110	86	193	2.39e-16	75.5
MS.gene81090.t1	MTR_5g012840	34.615	156	85	5	1	144	52	202	8.57e-16	73.9
MS.gene81090.t1	MTR_7g056277	32.298	161	100	3	8	162	334	491	6.83e-15	72.0
MS.gene81090.t1	MTR_7g056287	31.250	160	101	3	8	161	78	234	9.53e-15	71.2
MS.gene81090.t1	MTR_7g098570	41.667	120	61	4	1	112	83	201	1.20e-14	71.2
MS.gene81090.t1	MTR_4g080610	46.154	91	45	2	73	162	121	208	1.93e-14	70.5
MS.gene81090.t1	MTR_8g085430	36.719	128	66	6	1	117	82	205	2.47e-14	70.1
MS.gene81090.t1	MTR_1g041245	33.526	173	95	7	1	161	74	238	3.63e-14	69.7
MS.gene81090.t1	MTR_7g115270	32.278	158	86	4	7	143	81	238	3.94e-14	69.7
MS.gene81090.t1	MTR_1g066830	29.825	171	110	5	1	165	75	241	1.04e-13	68.6
MS.gene81090.t1	MTR_7g098480	35.374	147	78	6	9	144	89	229	1.24e-13	68.6
MS.gene81090.t1	MTR_6g011460	30.303	165	96	6	8	162	120	275	1.40e-13	68.2
MS.gene81090.t1	MTR_3g071850	35.971	139	76	5	12	141	104	238	1.66e-13	68.2
MS.gene81090.t1	MTR_7g056447	36.090	133	73	6	9	133	80	208	3.22e-13	66.6
MS.gene81090.t1	MTR_3g040700	33.333	156	92	5	1	145	106	260	5.11e-13	66.6
MS.gene81090.t1	MTR_7g032370	33.333	153	80	6	4	145	56	197	7.04e-13	65.9
MS.gene81090.t1	MTR_5g058100	35.256	156	86	5	8	148	83	238	1.17e-12	65.5
MS.gene81090.t1	MTR_7g060470	33.125	160	87	5	24	165	101	258	1.93e-12	64.7
MS.gene81090.t1	MTR_4g039440	32.500	120	71	3	49	163	151	265	3.11e-12	64.3
MS.gene81090.t1	MTR_1g108800	37.671	146	80	5	6	142	47	190	3.22e-12	63.9
MS.gene81090.t1	MTR_6g022500	35.099	151	88	5	8	150	175	323	3.28e-12	64.3
MS.gene81090.t1	MTR_7g056433	36.054	147	80	6	10	144	84	228	3.83e-12	63.9
MS.gene81090.t1	MTR_4g134150	39.091	110	63	3	7	112	79	188	4.67e-12	62.8
MS.gene81090.t1	MTR_6g045333	36.765	136	79	4	12	141	141	275	8.17e-12	63.2
MS.gene81090.t1	MTR_7g098510	34.177	158	82	7	1	144	81	230	8.86e-12	62.8
MS.gene81090.t1	MTR_7g007080	34.646	127	75	3	23	145	103	225	1.11e-11	62.8
MS.gene81090.t1	MTR_3g036250	32.258	155	95	4	1	145	68	222	3.39e-11	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 45 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATACCTTGTCGTGTGTTTC+TGG	0.090694	3.1:-85505820	MS.gene81090:CDS
TCCATCTGTTTCACATATTA+TGG	0.165550	3.1:-85505912	MS.gene81090:CDS
AAATTACTAGTTCAAATTTA+AGG	0.171670	3.1:-85505648	None:intergenic
TTAAATTTGAACTAGTAATT+TGG	0.235482	3.1:+85505650	None:intergenic
CACACGACAAGGTATCTTTA+TGG	0.236534	3.1:+85505827	None:intergenic
TCCCTACGGCTTTCATTCTA+AGG	0.250763	3.1:-85506031	MS.gene81090:CDS
AATTACTAGTTCAAATTTAA+GGG	0.323327	3.1:-85505647	None:intergenic
GCCAACAAGCTTATCCAATT+TGG	0.334639	3.1:-85505787	MS.gene81090:CDS
TCACATATTATGGACATATC+AGG	0.341356	3.1:-85505902	MS.gene81090:CDS
ATGTTATCCTCTTCAATTTG+AGG	0.360335	3.1:+85505991	None:intergenic
TGCTTCCAACTCTTGTAGAA+TGG	0.387005	3.1:+85505860	None:intergenic
AATGTTAGCTCTGACCAAAT+TGG	0.390378	3.1:+85505773	None:intergenic
TGTAGTAGTGACACTTGATA+TGG	0.393009	3.1:+85506135	None:intergenic
ATACCTTGTCGTGTGTTTCT+GGG	0.397938	3.1:-85505819	MS.gene81090:CDS
ATGTGAAACAGATGGATTCC+AGG	0.435339	3.1:+85505919	None:intergenic
GCTTCCAACTCTTGTAGAAT+GGG	0.440611	3.1:+85505861	None:intergenic
TTTGAGGACAGACAAGGTTT+CGG	0.443242	3.1:+85506007	None:intergenic
CGCAAGTAGGCGCATATGTT+GGG	0.450467	3.1:+85505704	None:intergenic
ACCAAATTGGATAAGCTTGT+TGG	0.450733	3.1:+85505786	None:intergenic
TAGCATTAGAAACAAATTTG+TGG	0.474187	3.1:+85506102	None:intergenic
ATTATGCATGGGAGAGATGT+TGG	0.483648	3.1:+85505749	None:intergenic
CCGCAAGTAGGCGCATATGT+TGG	0.484727	3.1:+85505703	None:intergenic
AGCGGGAGAGATCAATGGTT+AGG	0.516583	3.1:+85506060	None:intergenic
GTCGCTCAAACTCCTTCACC+TGG	0.548571	3.1:-85505937	MS.gene81090:CDS
CTTCAATTTGAGGACAGACA+AGG	0.555021	3.1:+85506001	None:intergenic
ATCTGAGCGGGAGAGATCAA+TGG	0.565352	3.1:+85506055	None:intergenic
ATGTCCCATTCTACAAGAGT+TGG	0.565975	3.1:-85505865	MS.gene81090:CDS
CTCCCGCTCAGATTTCCCTA+CGG	0.568994	3.1:-85506045	MS.gene81090:CDS
GCGATCAAGCAAATTATGCA+TGG	0.570982	3.1:+85505737	None:intergenic
AGCCGTAGGGAAATCTGAGC+GGG	0.573586	3.1:+85506043	None:intergenic
TCCATAATATGTGAAACAGA+TGG	0.573720	3.1:+85505911	None:intergenic
GACACTTGATATGGAATGAG+AGG	0.580314	3.1:+85506144	None:intergenic
AGCGACGGAAGATCAACGAC+AGG	0.590697	3.1:+85505954	None:intergenic
CGATCAAGCAAATTATGCAT+GGG	0.597728	3.1:+85505738	None:intergenic
TGTCTGTCCTCAAATTGAAG+AGG	0.599406	3.1:-85505998	MS.gene81090:CDS
TCCTTAGAATGAAAGCCGTA+GGG	0.602699	3.1:+85506030	None:intergenic
AACAGATGGATTCCAGGTGA+AGG	0.605969	3.1:+85505925	None:intergenic
GCATATGTTGGGCGTTACGA+AGG	0.606778	3.1:+85505715	None:intergenic
CCAACATATGCGCCTACTTG+CGG	0.611512	3.1:-85505703	MS.gene81090:CDS
GTCCTTAGAATGAAAGCCGT+AGG	0.619468	3.1:+85506029	None:intergenic
TTGATATGGAATGAGAGGAT+AGG	0.621762	3.1:+85506149	None:intergenic
GAACCCAGAAACACACGACA+AGG	0.636895	3.1:+85505816	None:intergenic
AGGATAACATTGAACTACGA+AGG	0.654558	3.1:-85505978	MS.gene81090:CDS
AGGTGAAGGAGTTTGAGCGA+CGG	0.665820	3.1:+85505939	None:intergenic
AAGCCGTAGGGAAATCTGAG+CGG	0.734235	3.1:+85506042	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTAAATTTGAACTAGTAATT+TGG	+	chr3.1:85506176-85506195	None:intergenic	15.0%
!	TAGCATTAGAAACAAATTTG+TGG	+	chr3.1:85505724-85505743	None:intergenic	25.0%
	ATGTTATCCTCTTCAATTTG+AGG	+	chr3.1:85505835-85505854	None:intergenic	30.0%
	TCACATATTATGGACATATC+AGG	-	chr3.1:85505921-85505940	MS.gene81090:CDS	30.0%
	TCCATAATATGTGAAACAGA+TGG	+	chr3.1:85505915-85505934	None:intergenic	30.0%
	TCCATCTGTTTCACATATTA+TGG	-	chr3.1:85505911-85505930	MS.gene81090:CDS	30.0%
!	CATATCAGGAAACTTTTATC+TGG	-	chr3.1:85505935-85505954	MS.gene81090:CDS	30.0%
!!!	AGGTTGAGTGTTATTTTGAT+TGG	+	chr3.1:85505657-85505676	None:intergenic	30.0%
	ACCAAATTGGATAAGCTTGT+TGG	+	chr3.1:85506040-85506059	None:intergenic	35.0%
	AGGATAACATTGAACTACGA+AGG	-	chr3.1:85505845-85505864	MS.gene81090:CDS	35.0%
	CGATCAAGCAAATTATGCAT+GGG	+	chr3.1:85506088-85506107	None:intergenic	35.0%
	TGTAGTAGTGACACTTGATA+TGG	+	chr3.1:85505691-85505710	None:intergenic	35.0%
	TTGATATGGAATGAGAGGAT+AGG	+	chr3.1:85505677-85505696	None:intergenic	35.0%
!	TTTTGATACATACCGCAAGT+AGG	+	chr3.1:85506135-85506154	None:intergenic	35.0%
!!	AATGTTAGCTCTGACCAAAT+TGG	+	chr3.1:85506053-85506072	None:intergenic	35.0%
	ATACCTTGTCGTGTGTTTCT+GGG	-	chr3.1:85506004-85506023	MS.gene81090:CDS	40.0%
	ATGTCCCATTCTACAAGAGT+TGG	-	chr3.1:85505958-85505977	MS.gene81090:CDS	40.0%
	ATTATGCATGGGAGAGATGT+TGG	+	chr3.1:85506077-85506096	None:intergenic	40.0%
	CTTCAATTTGAGGACAGACA+AGG	+	chr3.1:85505825-85505844	None:intergenic	40.0%
	GACACTTGATATGGAATGAG+AGG	+	chr3.1:85505682-85505701	None:intergenic	40.0%
	GCCAACAAGCTTATCCAATT+TGG	-	chr3.1:85506036-85506055	MS.gene81090:CDS	40.0%
	GCGATCAAGCAAATTATGCA+TGG	+	chr3.1:85506089-85506108	None:intergenic	40.0%
	GCTTCCAACTCTTGTAGAAT+GGG	+	chr3.1:85505965-85505984	None:intergenic	40.0%
	TCCTTAGAATGAAAGCCGTA+GGG	+	chr3.1:85505796-85505815	None:intergenic	40.0%
	TGCTTCCAACTCTTGTAGAA+TGG	+	chr3.1:85505966-85505985	None:intergenic	40.0%
	TGTCTGTCCTCAAATTGAAG+AGG	-	chr3.1:85505825-85505844	MS.gene81090:CDS	40.0%
	TTTGAGGACAGACAAGGTTT+CGG	+	chr3.1:85505819-85505838	None:intergenic	40.0%
!	ATGTGAAACAGATGGATTCC+AGG	+	chr3.1:85505907-85505926	None:intergenic	40.0%
!	CACACGACAAGGTATCTTTA+TGG	+	chr3.1:85505999-85506018	None:intergenic	40.0%
	GATACCTTGTCGTGTGTTTC+TGG	-	chr3.1:85506003-85506022	MS.gene81090:CDS	45.0%
	GTCCTTAGAATGAAAGCCGT+AGG	+	chr3.1:85505797-85505816	None:intergenic	45.0%
	TCCCTACGGCTTTCATTCTA+AGG	-	chr3.1:85505792-85505811	MS.gene81090:CDS	45.0%
!	AACAGATGGATTCCAGGTGA+AGG	+	chr3.1:85505901-85505920	None:intergenic	45.0%
	AAGCCGTAGGGAAATCTGAG+CGG	+	chr3.1:85505784-85505803	None:intergenic	50.0%
	AGCGGGAGAGATCAATGGTT+AGG	+	chr3.1:85505766-85505785	None:intergenic	50.0%
	AGGTGAAGGAGTTTGAGCGA+CGG	+	chr3.1:85505887-85505906	None:intergenic	50.0%
	ATCTGAGCGGGAGAGATCAA+TGG	+	chr3.1:85505771-85505790	None:intergenic	50.0%
	CCAACATATGCGCCTACTTG+CGG	-	chr3.1:85506120-85506139	MS.gene81090:CDS	50.0%
	CGCAAGTAGGCGCATATGTT+GGG	+	chr3.1:85506122-85506141	None:intergenic	50.0%
	GAACCCAGAAACACACGACA+AGG	+	chr3.1:85506010-85506029	None:intergenic	50.0%
	GCATATGTTGGGCGTTACGA+AGG	+	chr3.1:85506111-85506130	None:intergenic	50.0%
	AGCCGTAGGGAAATCTGAGC+GGG	+	chr3.1:85505783-85505802	None:intergenic	55.0%
	AGCGACGGAAGATCAACGAC+AGG	+	chr3.1:85505872-85505891	None:intergenic	55.0%
	CCGCAAGTAGGCGCATATGT+TGG	+	chr3.1:85506123-85506142	None:intergenic	55.0%
	CTCCCGCTCAGATTTCCCTA+CGG	-	chr3.1:85505778-85505797	MS.gene81090:CDS	55.0%
	GTCGCTCAAACTCCTTCACC+TGG	-	chr3.1:85505886-85505905	MS.gene81090:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	85505650	85506195	85505650	ID=MS.gene81090
chr3.1	mRNA	85505650	85506195	85505650	ID=MS.gene81090.t1;Parent=MS.gene81090
chr3.1	exon	85505650	85506195	85505650	ID=MS.gene81090.t1.exon1;Parent=MS.gene81090.t1
chr3.1	CDS	85505650	85506195	85505650	ID=cds.MS.gene81090.t1;Parent=MS.gene81090.t1

Gene Sequence

>MS.gene81090

ATGGCCAATCAAAATAACACTCAACCTATCCTCTCATTCCATATCAAGTGTCACTACTACAATTGTGATATCCACAAATTTGTTTCTAATGCTATTCAACGACGAGTTCAAAACCTAACCATTGATCTCTCCCGCTCAGATTTCCCTACGGCTTTCATTCTAAGGACCGAAACCTTGTCTGTCCTCAAATTGAAGAGGATAACATTGAACTACGAAGGTCCTGTCGTTGATCTTCCGTCGCTCAAACTCCTTCACCTGGAATCCATCTGTTTCACATATTATGGACATATCAGGAAACTTTTATCTGGATGTCCCATTCTACAAGAGTTGGAAGCAAATGATCTAACCATAAAGATACCTTGTCGTGTGTTTCTGGGTTCTTCACAGCCAACAAGCTTATCCAATTTGGTCAGAGCTAACATTTCCAACATCTCTCCCATGCATAATTTGCTTGATCGCCTTCGTAACGCCCAACATATGCGCCTACTTGCGGTATGTATCAAAATATTTTCTGCTTATATGACCAAATTACTAGTTCAAATTTAA

Protein sequence

>MS.gene81090.t1

MANQNNTQPILSFHIKCHYYNCDIHKFVSNAIQRRVQNLTIDLSRSDFPTAFILRTETLSVLKLKRITLNYEGPVVDLPSLKLLHLESICFTYYGHIRKLLSGCPILQELEANDLTIKIPCRVFLGSSQPTSLSNLVRANISNISPMHNLLDRLRNAQHMRLLAVCIKIFSAYMTKLLVQI