AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003455

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003455.t1 MTR_4g080787 70.202 396 110 2 1 391 102 494 0.0 536
MS.gene003455.t1 MTR_4g080773 65.657 396 131 1 1 391 102 497 2.13e-170 485
MS.gene003455.t1 MTR_4g080773 65.903 393 129 1 1 388 102 494 2.36e-169 483
MS.gene003455.t1 MTR_3g048290 63.918 388 135 2 1 384 96 482 1.55e-160 460
MS.gene003455.t1 MTR_4g080807 64.146 357 94 1 1 357 1 323 3.27e-154 441
MS.gene003455.t1 MTR_4g080590 47.439 371 190 4 1 370 90 456 1.71e-95 293
MS.gene003455.t1 MTR_5g039950 45.822 371 167 4 1 365 77 419 1.16e-90 279
MS.gene003455.t1 MTR_4g130200 42.350 366 179 2 1 365 57 391 2.01e-84 262
MS.gene003455.t1 MTR_7g023310 48.378 370 182 5 1 366 93 457 2.85e-84 264
MS.gene003455.t1 MTR_7g039630 50.526 285 135 3 1 279 59 343 2.68e-83 258
MS.gene003455.t1 MTR_4g080590 50.357 280 134 4 1 279 90 365 1.02e-70 227
MS.gene003455.t1 MTR_4g081030 39.769 347 153 4 19 365 97 387 8.61e-58 193
MS.gene003455.t1 MTR_5g039970 51.073 233 111 2 1 230 90 322 3.74e-56 187
MS.gene003455.t1 MTR_7g023330 44.610 269 140 4 101 365 132 395 2.58e-55 187
MS.gene003455.t1 MTR_2g094750 44.186 301 141 9 1 275 161 460 1.34e-53 185
MS.gene003455.t1 MTR_2g094450 39.459 185 94 5 105 286 30 199 2.99e-24 99.4
MS.gene003455.t1 MTR_4g032730 27.606 355 208 12 2 325 92 428 5.48e-19 89.0
MS.gene003455.t1 MTR_8g467800 27.746 346 211 11 1 325 88 415 3.94e-17 83.2
MS.gene003455.t1 MTR_1g082260 36.364 132 81 1 5 133 44 175 5.14e-17 79.0
MS.gene003455.t1 MTR_2g072530 23.676 321 226 7 20 333 103 411 3.84e-14 73.9
MS.gene003455.t1 MTR_5g080710 24.290 317 216 9 24 334 123 421 4.15e-14 73.9
MS.gene003455.t1 MTR_5g084070 27.469 324 194 10 24 334 100 395 6.04e-14 73.6
MS.gene003455.t1 MTR_2g102420 48.571 70 36 0 206 275 18 87 2.62e-11 60.1
MS.gene003455.t1 MTR_8g014260 27.576 330 207 13 38 356 131 439 4.45e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003455.t1 AT1G66290 27.794 349 225 13 2 338 96 429 8.31e-20 91.3
MS.gene003455.t1 AT1G78750 29.480 346 207 13 11 333 95 426 8.36e-20 91.3
MS.gene003455.t1 AT1G78750 29.480 346 207 13 11 333 95 426 8.36e-20 91.3
MS.gene003455.t1 AT5G22730 27.954 347 229 10 7 338 101 441 1.32e-19 90.5
MS.gene003455.t1 AT5G22730 27.954 347 229 10 7 338 101 441 1.32e-19 90.5
MS.gene003455.t1 AT4G14096 25.549 364 247 11 19 367 97 451 1.13e-18 87.8
MS.gene003455.t1 AT4G14096 25.549 364 247 11 19 367 97 451 1.13e-18 87.8
MS.gene003455.t1 AT1G06630 31.641 256 154 9 24 274 112 351 3.71e-18 85.5
MS.gene003455.t1 AT1G06630 31.641 256 154 9 24 274 112 351 3.71e-18 85.5
MS.gene003455.t1 AT5G53840 26.301 365 249 12 1 361 83 431 4.12e-18 86.3
MS.gene003455.t1 AT1G06630 31.641 256 154 9 24 274 112 351 5.57e-18 85.5
MS.gene003455.t1 AT1G06630 31.641 256 154 9 24 274 112 351 5.57e-18 85.5
MS.gene003455.t1 AT1G06630 31.641 256 154 9 24 274 112 351 5.57e-18 85.5
MS.gene003455.t1 AT1G06630 31.641 256 154 9 24 274 112 351 5.57e-18 85.5
MS.gene003455.t1 AT1G06630 31.641 256 154 9 24 274 121 360 6.29e-18 85.5
MS.gene003455.t1 AT4G14103 27.675 271 176 8 19 278 97 358 6.31e-18 85.1
MS.gene003455.t1 AT4G14103 27.675 271 176 8 19 278 97 358 6.31e-18 85.1
MS.gene003455.t1 AT5G22660 28.916 332 208 14 18 333 95 414 7.71e-18 85.5
MS.gene003455.t1 AT1G06630 31.641 256 154 9 24 274 112 351 1.17e-17 84.7
MS.gene003455.t1 AT3G59160 25.208 361 221 12 24 366 131 460 1.58e-17 84.3
MS.gene003455.t1 AT4G14103 27.925 265 171 8 19 272 97 352 7.61e-17 82.4
MS.gene003455.t1 AT1G66300 28.228 333 210 10 23 345 121 434 7.79e-17 82.4
MS.gene003455.t1 AT3G59200 24.129 402 253 13 1 367 81 465 8.15e-17 82.4
MS.gene003455.t1 AT3G60040 30.040 253 161 10 24 272 340 580 9.89e-17 82.4
MS.gene003455.t1 AT3G60040 30.040 253 161 10 24 272 338 578 1.01e-16 82.4
MS.gene003455.t1 AT3G60040 30.040 253 161 10 24 272 121 361 1.22e-16 82.0
MS.gene003455.t1 AT3G59180 26.854 391 227 15 24 367 70 448 1.30e-16 81.6
MS.gene003455.t1 AT1G69630 29.091 330 203 15 20 333 105 419 1.48e-16 81.3
MS.gene003455.t1 AT3G60040 30.040 253 161 10 24 272 340 580 1.65e-16 82.0
MS.gene003455.t1 AT3G60040 30.040 253 161 10 24 272 341 581 1.74e-16 82.0
MS.gene003455.t1 AT3G59200 27.517 298 173 11 1 272 81 361 1.97e-16 80.5
MS.gene003455.t1 AT1G16930 26.627 338 217 11 20 336 99 426 2.16e-16 80.9
MS.gene003455.t1 AT1G16930 26.627 338 217 11 20 336 99 426 2.16e-16 80.9
MS.gene003455.t1 AT1G16930 26.627 338 217 11 20 336 99 426 2.16e-16 80.9
MS.gene003455.t1 AT5G38590 26.879 346 217 11 1 337 69 387 2.20e-16 80.9
MS.gene003455.t1 AT3G59210 25.549 364 236 11 30 367 109 463 4.48e-16 80.1
MS.gene003455.t1 AT3G59210 25.549 364 236 11 30 367 109 463 4.48e-16 80.1
MS.gene003455.t1 AT3G59210 25.549 364 236 11 30 367 109 463 4.48e-16 80.1
MS.gene003455.t1 AT3G59210 25.549 364 236 11 30 367 109 463 4.48e-16 80.1
MS.gene003455.t1 AT3G59210 25.549 364 236 11 30 367 109 463 4.48e-16 80.1
MS.gene003455.t1 AT5G15620 28.313 332 211 12 22 334 84 407 1.08e-15 79.0
MS.gene003455.t1 AT3G59240 26.923 338 216 14 19 334 99 427 1.75e-15 78.6
MS.gene003455.t1 AT1G58310 27.202 386 234 13 19 367 114 489 1.76e-15 78.6
MS.gene003455.t1 AT3G49030 25.753 365 240 14 2 357 88 430 3.09e-15 77.4
MS.gene003455.t1 AT3G58960 30.515 272 168 12 30 285 97 363 3.83e-15 77.4
MS.gene003455.t1 AT3G58900 27.066 351 218 13 12 334 84 424 4.12e-15 77.0
MS.gene003455.t1 AT3G50710 29.560 318 172 13 52 349 124 409 5.08e-15 76.6
MS.gene003455.t1 AT1G78730 26.692 266 139 8 19 263 114 344 8.23e-15 75.9
MS.gene003455.t1 AT1G32375 28.136 295 183 8 47 333 117 390 8.37e-15 75.9
MS.gene003455.t1 AT1G32375 28.136 295 183 8 47 333 117 390 8.37e-15 75.9
MS.gene003455.t1 AT2G04230 28.022 364 234 11 1 353 85 431 1.24e-14 75.5
MS.gene003455.t1 AT2G26860 25.000 356 214 12 3 333 69 396 1.80e-14 74.7
MS.gene003455.t1 AT2G26860 25.000 356 214 12 3 333 69 396 1.80e-14 74.7
MS.gene003455.t1 AT1G56610 26.619 278 187 5 14 277 50 324 2.48e-14 74.7
MS.gene003455.t1 AT4G13985 26.197 355 228 15 9 346 102 439 2.79e-14 74.7
MS.gene003455.t1 AT1G56610 26.619 278 187 5 14 277 89 363 4.41e-14 73.9
MS.gene003455.t1 AT1G56610 26.207 290 197 5 2 277 140 426 4.45e-14 74.3
MS.gene003455.t1 AT5G22670 26.426 333 219 13 20 337 95 416 4.75e-14 73.9
MS.gene003455.t1 AT3G52680 27.211 294 170 8 50 328 138 402 1.12e-13 72.8
MS.gene003455.t1 AT4G22280 29.389 262 168 7 19 276 84 332 1.48e-13 72.0
MS.gene003455.t1 AT5G56700 23.373 338 222 11 1 325 29 342 7.05e-13 69.7
MS.gene003455.t1 AT4G22280 29.231 260 167 7 19 274 84 330 7.93e-13 69.3
MS.gene003455.t1 AT5G56390 24.779 339 213 12 42 365 113 424 8.88e-13 69.7
MS.gene003455.t1 AT3G26930 25.568 352 226 12 12 347 41 372 9.45e-13 69.7
MS.gene003455.t1 AT3G26930 25.568 352 226 12 12 347 86 417 1.05e-12 69.7
MS.gene003455.t1 AT3G62440 28.030 264 175 9 22 276 105 362 1.63e-12 68.9
MS.gene003455.t1 AT1G05080 25.496 353 220 14 1 333 79 408 2.20e-12 68.6
MS.gene003455.t1 AT1G55030 25.904 332 207 11 12 328 77 384 2.94e-12 68.2
MS.gene003455.t1 AT1G55030 25.904 332 207 11 12 328 87 394 3.00e-12 68.2
MS.gene003455.t1 AT2G42730 24.438 356 228 13 2 333 80 418 3.38e-12 68.2
MS.gene003455.t1 AT1G80470 27.046 281 189 8 26 300 105 375 3.86e-12 68.2
MS.gene003455.t1 AT5G62970 25.872 344 213 11 1 328 94 411 5.06e-12 67.4
MS.gene003455.t1 AT5G27750 25.086 291 198 8 59 333 138 424 7.09e-12 67.0
MS.gene003455.t1 AT5G56700 30.000 140 97 1 1 139 29 168 8.98e-12 65.9
MS.gene003455.t1 AT5G22730 27.076 277 187 7 7 272 101 373 1.10e-11 66.2
MS.gene003455.t1 AT1G13780 25.519 337 204 15 52 363 138 452 5.04e-11 64.3
MS.gene003455.t1 AT1G66320 25.461 271 176 9 81 337 175 433 5.43e-11 64.3
MS.gene003455.t1 AT5G25860 24.217 351 221 12 28 359 116 440 5.57e-11 64.3
MS.gene003455.t1 AT1G55660 25.667 300 189 9 52 333 176 459 6.18e-11 64.3
MS.gene003455.t1 AT5G22660 29.185 233 152 7 7 229 84 313 6.39e-11 63.5
MS.gene003455.t1 AT5G53635 26.989 352 222 13 4 333 65 403 9.70e-11 63.5

Find 61 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACCTTGTACAATTGTTAT+TGG 0.244477 4.3:-32606282 MS.gene003455:CDS
ACTACGGAGGTCAAGTTAAC+TGG 0.323880 4.3:+32606097 None:intergenic
TGAAGTGTTTGCTGGGATTC+CGG 0.334316 4.3:-32605745 MS.gene003455:CDS
GACCTTGTACAATTGTTATT+GGG 0.339260 4.3:-32606281 MS.gene003455:CDS
GTGGAAGTTCTTGAGATTCC+TGG 0.341747 4.3:-32605675 MS.gene003455:CDS
CAGAAACGACTAATTTCTTC+AGG 0.343491 4.3:+32606371 None:intergenic
TCAAGATAAACTTAACGAAT+TGG 0.345412 4.3:+32605437 None:intergenic
CTTCACAACAGAATCTATTA+AGG 0.349842 4.3:+32606543 None:intergenic
GAGAGAAGATTTGAAACAAC+AGG 0.360677 4.3:+32605504 None:intergenic
AAGGTCAACTCTTGTAAAAC+AGG 0.363675 4.3:+32606298 None:intergenic
ATGAGAAGGTGGATGATATC+TGG 0.370444 4.3:-32605354 MS.gene003455:CDS
ATCTCAAGAACTTCCACTTT+AGG 0.379741 4.3:+32605681 None:intergenic
TCATTGACCAAACTGTGTTT+AGG 0.394125 4.3:-32606439 MS.gene003455:CDS
CTTCCACTTTAGGAGCCTTT+CGG 0.394536 4.3:+32605691 None:intergenic
CTTGAGTCCTAAACACAGTT+TGG 0.414655 4.3:+32606432 None:intergenic
TGATTAGGTACTTAACTACT+TGG 0.425971 4.3:-32605557 MS.gene003455:intron
TTCAAAATCGATGGTCAACT+TGG 0.438130 4.3:+32606219 None:intergenic
GTCCATCAGTACTTCATTAG+TGG 0.440471 4.3:+32605719 None:intergenic
ACACATCTTGAAGTGTTTGC+TGG 0.440808 4.3:-32605753 MS.gene003455:CDS
AGATTCATCGTCGTAGTAGC+TGG 0.457076 4.3:+32604124 None:intergenic
GGCCTCCTACATCATCCAAT+TGG 0.473236 4.3:+32605329 None:intergenic
CACATCTTGAAGTGTTTGCT+GGG 0.475981 4.3:-32605752 MS.gene003455:CDS
GCAGCATATGATTCACTACT+TGG 0.483641 4.3:+32606043 None:intergenic
GGATGATATCTGGAACCAAT+TGG 0.496991 4.3:-32605344 MS.gene003455:CDS
TCTCTGGTGCTTCCTGCTGC+AGG 0.500502 4.3:-32603930 MS.gene003455:intron
TCTCCGAAAGGCTCCTAAAG+TGG 0.514005 4.3:-32605694 MS.gene003455:CDS
AAAATAAAACGTACCACTCC+AGG 0.522108 4.3:+32605657 None:intergenic
AGTACTTCATTAGTGGTCTC+CGG 0.531587 4.3:+32605726 None:intergenic
TGTCCATTCTTACCTGCAGC+AGG 0.534614 4.3:+32603918 None:intergenic
TGTTAAATGTGGGAAGGAGA+TGG 0.538548 4.3:+32605781 None:intergenic
ATTGGATGATGTAGGAGGCC+CGG 0.545520 4.3:-32605326 MS.gene003455:CDS
ACGACTAATTTCTTCAGGCT+AGG 0.552145 4.3:+32606376 None:intergenic
CAAGATCTTGAACTTTCACT+AGG 0.553456 4.3:-32606511 MS.gene003455:CDS
TAGGTACTTAACTACTTGGA+TGG 0.558260 4.3:-32605553 MS.gene003455:CDS
ATGTGTCAAATTGTTAAATG+TGG 0.562573 4.3:+32605770 None:intergenic
ATCAATAGATGCATCAACTA+CGG 0.563483 4.3:+32606081 None:intergenic
GACCACTAATGAAGTACTGA+TGG 0.566068 4.3:-32605721 MS.gene003455:CDS
CTACGGAGGTCAAGTTAACT+GGG 0.576215 4.3:+32606098 None:intergenic
CTAGGAAAACGAATACCACT+AGG 0.580426 4.3:+32606394 None:intergenic
CATATGATTCACTACTTGGA+TGG 0.581308 4.3:+32606047 None:intergenic
ATTAGCTGATAATGAGAAGG+TGG 0.584272 4.3:-32605365 MS.gene003455:CDS
GCAAAAGAGTTCAAAATCGA+TGG 0.584518 4.3:+32606210 None:intergenic
TTTATTAGCTGATAATGAGA+AGG 0.585950 4.3:-32605368 MS.gene003455:CDS
AACTGACATCTCATCTGCAA+AGG 0.586977 4.3:+32606342 None:intergenic
CTCCCAATAACAATTGTACA+AGG 0.587766 4.3:+32606279 None:intergenic
TGTGTCAAATTGTTAAATGT+GGG 0.588530 4.3:+32605771 None:intergenic
GATGGTCAACTTGGTTAATG+TGG 0.589550 4.3:+32606228 None:intergenic
AGTTCTTGAGATTCCTGGAG+TGG 0.597460 4.3:-32605670 MS.gene003455:intron
TGGAACCAATTGGATGATGT+AGG 0.597577 4.3:-32605334 MS.gene003455:CDS
ACTATATAATAACACTCTTG+AGG 0.601018 4.3:-32605969 MS.gene003455:intron
TTTGATCTCCCCTAAATATG+GGG 0.602591 4.3:+32605408 None:intergenic
TGTGTATTTGAGCTATTGAG+TGG 0.623146 4.3:-32606016 MS.gene003455:CDS
AACCAATTGGATGATGTAGG+AGG 0.635148 4.3:-32605331 MS.gene003455:CDS
TCAAATTGTTAAATGTGGGA+AGG 0.635959 4.3:+32605775 None:intergenic
ACTGATGGACATTCTCCGAA+AGG 0.652765 4.3:-32605706 MS.gene003455:CDS
CTAGTGTCTTTACTACACAA+AGG 0.654654 4.3:-32605811 MS.gene003455:intron
TCGAAAGATGAGAAACTATG+TGG 0.659887 4.3:+32606463 None:intergenic
AGGTCAACTCTTGTAAAACA+GGG 0.670121 4.3:+32606299 None:intergenic
AGAGAAGATTTGAAACAACA+GGG 0.683271 4.3:+32605505 None:intergenic
TTCACTCTACATATTCCTAG+TGG 0.693154 4.3:-32606409 MS.gene003455:CDS
AATAGATGCATCAACTACGG+AGG 0.700718 4.3:+32606084 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATATGTAATTAAATGAT+TGG - chr4.3:32605338-32605357 MS.gene003455:CDS 10.0%
!! TCATTTAATTACATATATTT+AGG + chr4.3:32605337-32605356 None:intergenic 10.0%
!! TTGATAAAAAACATAAATTA+AGG + chr4.3:32605754-32605773 None:intergenic 10.0%
!!! TATGCTTTTTAAATTTTTAT+GGG - chr4.3:32604891-32604910 MS.gene003455:intron 10.0%
!!! TTATGCTTTTTAAATTTTTA+TGG - chr4.3:32604890-32604909 MS.gene003455:intron 10.0%
!! ATACTTACACTTTACATAAT+AGG - chr4.3:32606285-32606304 MS.gene003455:CDS 20.0%
!! ATTAAAGGAAATAGCATAAT+AGG + chr4.3:32606551-32606570 None:intergenic 20.0%
!! TAACTGATAAATTCATGAAA+AGG - chr4.3:32605541-32605560 MS.gene003455:CDS 20.0%
!! TAAGTATGGTTTAAATATGT+CGG + chr4.3:32605290-32605309 None:intergenic 20.0%
!!! ATATTTTTAGTATGTGAAGT+AGG - chr4.3:32605907-32605926 MS.gene003455:intron 20.0%
!!! ATGTAATTAAATGATTGGTT+AGG - chr4.3:32605343-32605362 MS.gene003455:CDS 20.0%
!!! TTTTTAAAACTAGAGTCTTT+CGG - chr4.3:32605820-32605839 MS.gene003455:intron 20.0%
! AAATCTGAAGGAACTAATTT+AGG + chr4.3:32605964-32605983 None:intergenic 25.0%
! AAATGTACCTAGTCTATATT+GGG - chr4.3:32605660-32605679 MS.gene003455:intron 25.0%
! AAGTATGGTTTAAATATGTC+GGG + chr4.3:32605289-32605308 None:intergenic 25.0%
! ACTAACCAAATATATGAACT+CGG + chr4.3:32605612-32605631 None:intergenic 25.0%
! ACTATATAATAACACTCTTG+AGG - chr4.3:32604396-32604415 MS.gene003455:intron 25.0%
! ATGTGTCAAATTGTTAAATG+TGG + chr4.3:32604598-32604617 None:intergenic 25.0%
! ATTCCATAATATACTTAGAG+TGG - chr4.3:32605149-32605168 MS.gene003455:intron 25.0%
! CATAAAAAGCAAAGACAATA+AGG + chr4.3:32606507-32606526 None:intergenic 25.0%
! GACATATTTAAACCATACTT+AGG - chr4.3:32605289-32605308 MS.gene003455:intron 25.0%
! GAGAAAATAATTAATGAGTC+AGG + chr4.3:32606477-32606496 None:intergenic 25.0%
! TAAATTAGTTCCTTCAGATT+TGG - chr4.3:32605963-32605982 MS.gene003455:intron 25.0%
! TCAAGATAAACTTAACGAAT+TGG + chr4.3:32604931-32604950 None:intergenic 25.0%
! TCTCATCTCATATAGATTAA+AGG + chr4.3:32606566-32606585 None:intergenic 25.0%
! TGAAAAATTCCCCATATTTA+GGG - chr4.3:32604948-32604967 MS.gene003455:intron 25.0%
! TGTGTCAAATTGTTAAATGT+GGG + chr4.3:32604597-32604616 None:intergenic 25.0%
! TTGAAAAATTCCCCATATTT+AGG - chr4.3:32604947-32604966 MS.gene003455:intron 25.0%
! TTGTTTAGTGATTCTTGATT+AGG - chr4.3:32604793-32604812 MS.gene003455:intron 25.0%
!! ACCTTCAAATTAAAAGTATG+TGG - chr4.3:32605860-32605879 MS.gene003455:intron 25.0%
!! TTTATTAGCTGATAATGAGA+AGG - chr4.3:32604997-32605016 MS.gene003455:intron 25.0%
!!! ACAAGTTCTAGATTCTTTTT+GGG + chr4.3:32605225-32605244 None:intergenic 25.0%
!!! ACTTTTCTTTTGATTTGACT+TGG - chr4.3:32606053-32606072 MS.gene003455:CDS 25.0%
!!! ATTGTCTTTGCTTTTTATGA+AGG - chr4.3:32606508-32606527 MS.gene003455:CDS 25.0%
!!! CTTTTCTTTTGATTTGACTT+GGG - chr4.3:32606054-32606073 MS.gene003455:CDS 25.0%
AAAAAATCGATGACACAATC+CGG + chr4.3:32605061-32605080 None:intergenic 30.0%
AATGGAAACAAAACTACGTT+AGG + chr4.3:32604533-32604552 None:intergenic 30.0%
ATAACAGGACAAGTTATTAG+TGG - chr4.3:32606341-32606360 MS.gene003455:CDS 30.0%
ATCAATAGATGCATCAACTA+CGG + chr4.3:32604287-32604306 None:intergenic 30.0%
ATTCATGAAAAGGTAATGCA+AGG - chr4.3:32605551-32605570 MS.gene003455:CDS 30.0%
CAAATGTACCTAGTCTATAT+TGG - chr4.3:32605659-32605678 MS.gene003455:intron 30.0%
CAACCACTCTAAGTATATTA+TGG + chr4.3:32605155-32605174 None:intergenic 30.0%
CATGAAGATGATAAGCAAAT+AGG + chr4.3:32604430-32604449 None:intergenic 30.0%
CATTCAATCATACCTAAGTA+TGG + chr4.3:32605304-32605323 None:intergenic 30.0%
CTAAAGTCAACATTCTCATT+AGG + chr4.3:32604466-32604485 None:intergenic 30.0%
CTTCACAACAGAATCTATTA+AGG + chr4.3:32603825-32603844 None:intergenic 30.0%
GAAAAATTCCCCATATTTAG+GGG - chr4.3:32604949-32604968 MS.gene003455:intron 30.0%
TCAAATTGTTAAATGTGGGA+AGG + chr4.3:32604593-32604612 None:intergenic 30.0%
TGACCTTGTACAATTGTTAT+TGG - chr4.3:32604083-32604102 MS.gene003455:intron 30.0%
TGATTAGGTACTTAACTACT+TGG - chr4.3:32604808-32604827 MS.gene003455:intron 30.0%
TGTAGTAAAGACACTAGTAA+TGG + chr4.3:32604551-32604570 None:intergenic 30.0%
! AAATGATTGGTTAGGTCTTT+AGG - chr4.3:32605351-32605370 MS.gene003455:CDS 30.0%
! AGAGAAGATTTGAAACAACA+GGG + chr4.3:32604863-32604882 None:intergenic 30.0%
! CTATATGAGATGAGATTTTG+TGG - chr4.3:32606571-32606590 MS.gene003455:CDS 30.0%
! GACCTTGTACAATTGTTATT+GGG - chr4.3:32604084-32604103 MS.gene003455:intron 30.0%
! TTTTGATCTCCCCTAAATAT+GGG + chr4.3:32604961-32604980 None:intergenic 30.0%
!! ATAATGGAGAGGAAACTTTT+TGG - chr4.3:32605392-32605411 MS.gene003455:CDS 30.0%
!! ATTTTGATCTCCCCTAAATA+TGG + chr4.3:32604962-32604981 None:intergenic 30.0%
!! TTCTTTGGAATAAGTTTCAC+AGG - chr4.3:32606006-32606025 MS.gene003455:CDS 30.0%
!!! CAAGTTCTAGATTCTTTTTG+GGG + chr4.3:32605224-32605243 None:intergenic 30.0%
!!! GACAAGTTCTAGATTCTTTT+TGG + chr4.3:32605226-32605245 None:intergenic 30.0%
!!! GCCACATACTTTTAATTTGA+AGG + chr4.3:32605864-32605883 None:intergenic 30.0%
AAAAATCGATGACACAATCC+GGG + chr4.3:32605060-32605079 None:intergenic 35.0%
AAAATAAAACGTACCACTCC+AGG + chr4.3:32604711-32604730 None:intergenic 35.0%
AAGGTCAACTCTTGTAAAAC+AGG + chr4.3:32604070-32604089 None:intergenic 35.0%
AGAGATAGACGCTTAAATGT+GGG - chr4.3:32605479-32605498 MS.gene003455:CDS 35.0%
AGATGTGTGTGTGATTATAG+AGG - chr4.3:32606151-32606170 MS.gene003455:CDS 35.0%
AGGTCAACTCTTGTAAAACA+GGG + chr4.3:32604069-32604088 None:intergenic 35.0%
ATCTCAAGAACTTCCACTTT+AGG + chr4.3:32604687-32604706 None:intergenic 35.0%
ATGTGTGTGTGATTATAGAG+GGG - chr4.3:32606153-32606172 MS.gene003455:CDS 35.0%
CAAGATCTTGAACTTTCACT+AGG - chr4.3:32603854-32603873 MS.gene003455:intron 35.0%
CAGAAACGACTAATTTCTTC+AGG + chr4.3:32603997-32604016 None:intergenic 35.0%
CATATGATTCACTACTTGGA+TGG + chr4.3:32604321-32604340 None:intergenic 35.0%
CCAAAAATCGTGTTATGCTT+CGG + chr4.3:32605201-32605220 None:intergenic 35.0%
CTAGTGTCTTTACTACACAA+AGG - chr4.3:32604554-32604573 MS.gene003455:intron 35.0%
CTCCCAATAACAATTGTACA+AGG + chr4.3:32604089-32604108 None:intergenic 35.0%
GATGTGTGTGTGATTATAGA+GGG - chr4.3:32606152-32606171 MS.gene003455:CDS 35.0%
GCAAAAGAGTTCAAAATCGA+TGG + chr4.3:32604158-32604177 None:intergenic 35.0%
GCTATCCGAGTTCATATATT+TGG - chr4.3:32605604-32605623 MS.gene003455:intron 35.0%
TAGATGTTGGAACACTTAGT+CGG - chr4.3:32605453-32605472 MS.gene003455:CDS 35.0%
TAGGTACTTAACTACTTGGA+TGG - chr4.3:32604812-32604831 MS.gene003455:intron 35.0%
TATGTCGGGATGTTATAGTA+AGG + chr4.3:32605275-32605294 None:intergenic 35.0%
TCATTGACCAAACTGTGTTT+AGG - chr4.3:32603926-32603945 MS.gene003455:intron 35.0%
TCGAAAGATGAGAAACTATG+TGG + chr4.3:32603905-32603924 None:intergenic 35.0%
TGTGTATTTGAGCTATTGAG+TGG - chr4.3:32604349-32604368 MS.gene003455:intron 35.0%
TGTGTGAAGTATGGACATAA+TGG - chr4.3:32605376-32605395 MS.gene003455:CDS 35.0%
TTCAAAATCGATGGTCAACT+TGG + chr4.3:32604149-32604168 None:intergenic 35.0%
TTCACTCTACATATTCCTAG+TGG - chr4.3:32603956-32603975 MS.gene003455:intron 35.0%
TTTGATCTCCCCTAAATATG+GGG + chr4.3:32604960-32604979 None:intergenic 35.0%
! AAGGAGAAGAAAGTTGATTG+AGG + chr4.3:32606094-32606113 None:intergenic 35.0%
! ATTAGCTGATAATGAGAAGG+TGG - chr4.3:32605000-32605019 MS.gene003455:intron 35.0%
! CAGTTCTACAACTTTTCTCT+GGG - chr4.3:32604041-32604060 MS.gene003455:intron 35.0%
! GAGAGAAGATTTGAAACAAC+AGG + chr4.3:32604864-32604883 None:intergenic 35.0%
! GATTTTGAACTCTTTTGCGT+TGG - chr4.3:32604160-32604179 MS.gene003455:intron 35.0%
! TCAGTTCTACAACTTTTCTC+TGG - chr4.3:32604040-32604059 MS.gene003455:intron 35.0%
! TTAGTGGCTGTTTCATTGTT+AGG - chr4.3:32606357-32606376 MS.gene003455:CDS 35.0%
!! CTTTAGGATTGTGTGAAGTA+TGG - chr4.3:32605367-32605386 MS.gene003455:CDS 35.0%
!! TGCTTGTAATTGCTGTTTCA+AGG - chr4.3:32604740-32604759 MS.gene003455:intron 35.0%
!! TTTTCAGGCTTCAAATTCTC+TGG - chr4.3:32606419-32606438 MS.gene003455:CDS 35.0%
!!! AGGAAACTTTTTGGTTTCAG+CGG - chr4.3:32605401-32605420 MS.gene003455:CDS 35.0%
!!! TAACGTTCACTGTTGTTTTC+AGG - chr4.3:32606404-32606423 MS.gene003455:CDS 35.0%
AACCAATTGGATGATGTAGG+AGG - chr4.3:32605034-32605053 MS.gene003455:intron 40.0%
AACTGACATCTCATCTGCAA+AGG + chr4.3:32604026-32604045 None:intergenic 40.0%
AATAGATGCATCAACTACGG+AGG + chr4.3:32604284-32604303 None:intergenic 40.0%
AATCCAGCAACCAAATCTGA+AGG + chr4.3:32605976-32605995 None:intergenic 40.0%
ACGACTAATTTCTTCAGGCT+AGG + chr4.3:32603992-32604011 None:intergenic 40.0%
AGTACTTCATTAGTGGTCTC+CGG + chr4.3:32604642-32604661 None:intergenic 40.0%
ATAGTAAGGCAAAACCACCA+AGG + chr4.3:32605261-32605280 None:intergenic 40.0%
ATGAGAAGGTGGATGATATC+TGG - chr4.3:32605011-32605030 MS.gene003455:intron 40.0%
CTAGGAAAACGAATACCACT+AGG + chr4.3:32603974-32603993 None:intergenic 40.0%
CTAGTTCCTATGTGGAAAGT+GGG - chr4.3:32605504-32605523 MS.gene003455:CDS 40.0%
CTTGAGTCCTAAACACAGTT+TGG + chr4.3:32603936-32603955 None:intergenic 40.0%
GAAACAGCAATTACAAGCAC+TGG + chr4.3:32604739-32604758 None:intergenic 40.0%
GAGAGATAGACGCTTAAATG+TGG - chr4.3:32605478-32605497 MS.gene003455:CDS 40.0%
GATGGTCAACTTGGTTAATG+TGG + chr4.3:32604140-32604159 None:intergenic 40.0%
GCAGCATATGATTCACTACT+TGG + chr4.3:32604325-32604344 None:intergenic 40.0%
GTCCATCAGTACTTCATTAG+TGG + chr4.3:32604649-32604668 None:intergenic 40.0%
GTGTTCCAACATCTAAGCAT+TGG + chr4.3:32605448-32605467 None:intergenic 40.0%
TGCTTATGATCTCAAGCAAG+TGG + chr4.3:32605686-32605705 None:intergenic 40.0%
TGGAACCAATTGGATGATGT+AGG - chr4.3:32605031-32605050 MS.gene003455:intron 40.0%
TGTTAAATGTGGGAAGGAGA+TGG + chr4.3:32604587-32604606 None:intergenic 40.0%
TTCAGCCAATGCTTAGATGT+TGG - chr4.3:32605440-32605459 MS.gene003455:CDS 40.0%
TTGTCTTTCCAACTACACCT+TGG - chr4.3:32605241-32605260 MS.gene003455:intron 40.0%
TTTCGTTGCTGGAGACATAT+GGG - chr4.3:32606118-32606137 MS.gene003455:CDS 40.0%
! ACACATCTTGAAGTGTTTGC+TGG - chr4.3:32604612-32604631 MS.gene003455:intron 40.0%
! CACATCTTGAAGTGTTTGCT+GGG - chr4.3:32604613-32604632 MS.gene003455:intron 40.0%
! CCGAAGCATAACACGATTTT+TGG - chr4.3:32605198-32605217 MS.gene003455:intron 40.0%
! GAAGTATGGACATAATGGAG+AGG - chr4.3:32605381-32605400 MS.gene003455:CDS 40.0%
! GGATGATATCTGGAACCAAT+TGG - chr4.3:32605021-32605040 MS.gene003455:intron 40.0%
! GGATTAAGTTGCAGGTTCTT+TGG - chr4.3:32605991-32606010 MS.gene003455:CDS 40.0%
! TTTTCGTTGCTGGAGACATA+TGG - chr4.3:32606117-32606136 MS.gene003455:CDS 40.0%
!! GACCACTAATGAAGTACTGA+TGG - chr4.3:32604644-32604663 MS.gene003455:intron 40.0%
AAACCTCCCACTTTCCACAT+AGG + chr4.3:32605513-32605532 None:intergenic 45.0%
ACTACGGAGGTCAAGTTAAC+TGG + chr4.3:32604271-32604290 None:intergenic 45.0%
AGATTCATCGTCGTAGTAGC+TGG + chr4.3:32606244-32606263 None:intergenic 45.0%
AGTTCTTGAGATTCCTGGAG+TGG - chr4.3:32604695-32604714 MS.gene003455:intron 45.0%
CAAAACCACCAAGGTGTAGT+TGG + chr4.3:32605252-32605271 None:intergenic 45.0%
CAACAGTGAACGTTAGACCA+AGG + chr4.3:32606399-32606418 None:intergenic 45.0%
CAAGTGGCCCAATATAGACT+AGG + chr4.3:32605670-32605689 None:intergenic 45.0%
CCACTTTCCACATAGGAACT+AGG + chr4.3:32605506-32605525 None:intergenic 45.0%
CCTAGTTCCTATGTGGAAAG+TGG - chr4.3:32605503-32605522 MS.gene003455:CDS 45.0%
CTACGGAGGTCAAGTTAACT+GGG + chr4.3:32604270-32604289 None:intergenic 45.0%
GGTAATGCAAGGTGAACTTG+AGG - chr4.3:32605562-32605581 MS.gene003455:intron 45.0%
GTGGAAGTTCTTGAGATTCC+TGG - chr4.3:32604690-32604709 MS.gene003455:intron 45.0%
TCTCCAGCAACGAAAAGCAA+AGG + chr4.3:32606113-32606132 None:intergenic 45.0%
TCTTTCCAACTACACCTTGG+TGG - chr4.3:32605244-32605263 MS.gene003455:intron 45.0%
TGGAGACATATGGGGGAATT+TGG - chr4.3:32606127-32606146 MS.gene003455:CDS 45.0%
TTCAGCGGTATGTTTAAGCG+TGG - chr4.3:32605416-32605435 MS.gene003455:CDS 45.0%
TTCGTTGCTGGAGACATATG+GGG - chr4.3:32606119-32606138 MS.gene003455:CDS 45.0%
! ACTGATGGACATTCTCCGAA+AGG - chr4.3:32604659-32604678 MS.gene003455:intron 45.0%
! CTTCCACTTTAGGAGCCTTT+CGG + chr4.3:32604677-32604696 None:intergenic 45.0%
! GTGGCTGTTTCATTGTTAGG+TGG - chr4.3:32606360-32606379 MS.gene003455:CDS 45.0%
! GTTCCTTCAGATTTGGTTGC+TGG - chr4.3:32605970-32605989 MS.gene003455:CDS 45.0%
! TGAAGTGTTTGCTGGGATTC+CGG - chr4.3:32604620-32604639 MS.gene003455:intron 45.0%
! TGGTTGCTGGATTAAGTTGC+AGG - chr4.3:32605983-32606002 MS.gene003455:CDS 45.0%
!! TCTCCTTTGCTTTTCGTTGC+TGG - chr4.3:32606107-32606126 MS.gene003455:CDS 45.0%
!!! TTTTTTTTTTAAATAATAAC+AGG - chr4.3:32606326-32606345 MS.gene003455:CDS 5.0%
ATTGGATGATGTAGGAGGCC+CGG - chr4.3:32605039-32605058 MS.gene003455:intron 50.0%
CTTCCTGCTGCAGGTAAGAA+TGG - chr4.3:32606444-32606463 MS.gene003455:CDS 50.0%
GGCCTCCTACATCATCCAAT+TGG + chr4.3:32605039-32605058 None:intergenic 50.0%
GTGGAACACATTGAGTGCCT+TGG - chr4.3:32606379-32606398 MS.gene003455:CDS 50.0%
GTTCCTATGTGGAAAGTGGG+AGG - chr4.3:32605507-32605526 MS.gene003455:CDS 50.0%
TCGTTGCTGGAGACATATGG+GGG - chr4.3:32606120-32606139 MS.gene003455:CDS 50.0%
TCTCCGAAAGGCTCCTAAAG+TGG - chr4.3:32604671-32604690 MS.gene003455:intron 50.0%
TGTCCATTCTTACCTGCAGC+AGG + chr4.3:32606450-32606469 None:intergenic 50.0%
TGTGGGTCCTAGTTCCTATG+TGG - chr4.3:32605496-32605515 MS.gene003455:CDS 50.0%
TCTCTGGTGCTTCCTGCTGC+AGG - chr4.3:32606435-32606454 MS.gene003455:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 32603746 32606641 32603746 ID=MS.gene003455
chr4.3 mRNA 32603746 32606641 32603746 ID=MS.gene003455.t1;Parent=MS.gene003455
chr4.3 exon 32605970 32606641 32605970 ID=MS.gene003455.t1.exon1;Parent=MS.gene003455.t1
chr4.3 CDS 32605970 32606641 32605970 ID=cds.MS.gene003455.t1;Parent=MS.gene003455.t1
chr4.3 exon 32605671 32605829 32605671 ID=MS.gene003455.t1.exon2;Parent=MS.gene003455.t1
chr4.3 CDS 32605671 32605829 32605671 ID=cds.MS.gene003455.t1;Parent=MS.gene003455.t1
chr4.3 exon 32605307 32605572 32605307 ID=MS.gene003455.t1.exon3;Parent=MS.gene003455.t1
chr4.3 CDS 32605307 32605572 32605307 ID=cds.MS.gene003455.t1;Parent=MS.gene003455.t1
chr4.3 exon 32604112 32604148 32604112 ID=MS.gene003455.t1.exon4;Parent=MS.gene003455.t1
chr4.3 CDS 32604112 32604148 32604112 ID=cds.MS.gene003455.t1;Parent=MS.gene003455.t1
chr4.3 exon 32603931 32603961 32603931 ID=MS.gene003455.t1.exon5;Parent=MS.gene003455.t1
chr4.3 CDS 32603931 32603961 32603931 ID=cds.MS.gene003455.t1;Parent=MS.gene003455.t1
chr4.3 exon 32603746 32603756 32603746 ID=MS.gene003455.t1.exon6;Parent=MS.gene003455.t1
chr4.3 CDS 32603746 32603756 32603746 ID=cds.MS.gene003455.t1;Parent=MS.gene003455.t1
Gene Sequence

>MS.gene003455

ATGCACAAATCTAATCGAGTAGAAAAGCTTTGTGTTCAAACATTTGAAAGTGTTGTTGATGTAAACAAAGTTAGTTCCTTAATAGATTCTGTTGTGAAGCACAAAGTACAAGATCTTGAACTTTCACTAGGTGATCGAAATGATGAGTTTGTGTTACCACATAGTTTCTCATCTTTCGAGTCATTGACCAAACTGTGTTTAGGACTCAAGTTCACTCTACATATTCCTAGTGGTATTCGTTTTCCTAGCCTGAAGAAATTAGTCGTTTCTGAAGTTACCTTTGCAGATGAGATGTCAGTTCTACAACTTTTCTCTGGGTGCCCTGTTTTACAAGAGTTGACCTTGTACAATTGTTATTGGGAGAATATAGAGCAGATCACTGTTGCAATTTCCACATTAACCAAGTTGACCATCGATTTTGAACTCTTTTGCGTTGGTTATGATCATGATATGACACTCACGATTGATGCTGTGAATCTTTTATCGTTAAGTTGCAGATGCATTCAAACAATAGAATTTATCCCAGTTAACTTGACCTCCGTAGTTGATGCATCTATTGATCTTGAGTATGACCATCCAAGTAGTGAATCATATGCTGCTCACTGTGTATTTGAGCTATTGAGTGGACTTAGTAGTGTTAAGTCTCTCACACTATATAATAACACTCTTGAGGTATGTTTGCCTATTTGCTTATCATCTTCATGTTTTTTTATAATACCTAATGAGAATGTTGACTTTAGTTATTTGTTTCTTATAATCGTTGCATATTTTCTGTATTACAGTACCTAACGTAGTTTTGTTTCCATTACTAGTGTCTTTACTACACAAAGGATACTCTCCATCTCCTTCCCACATTTAACAATTTGACACATCTTGAAGTGTTTGCTGGGATTCCGGAGACCACTAATGAAGTACTGATGGACATTCTCCGAAAGGCTCCTAAAGTGGAAGTTCTTGAGATTCCTGGAGTGGTACGTTTTATTTTCTTCACCAGTGCTTGTAATTGCTGTTTCAAGGCTAACAATAATATTATTTCAGTCAATTGACTTGTTTAGTGATTCTTGATTAGGTACTTAACTACTTGGATGGTGAAGATTTGATATTGAACGCAGTGCCCTGTTGTTTCAAATCTTCTCTCAATCGATTATGCTTTTTAAATTTTTATGGGAATGAATATGAAATCCAATTCGTTAAGTTTATCTTGAAAAATTCCCCATATTTAGGGGAGATCAAAATACACTGTTCAAGACATTTATTAGCTGATAATGAGAAGGTGGATGATATCTGGAACCAATTGGATGATGTAGGAGGCCCGGATTGTGTCATCGATTTTTTGTAAATATTCTTTCTTTTCTTCTCTCTTTTTTTTTTGTTGAAGTGATTCAGTTTACTAATTTAACTTCATTCCATAATATACTTAGAGTGGTTGTTCTTACTTTTACACTTGACATACCGAAGCATAACACGATTTTTGGCCCCAAAAAGAATCTAGAACTTGTCTTTCCAACTACACCTTGGTGGTTTTGCCTTACTATAACATCCCGACATATTTAAACCATACTTAGGTATGATTGAATGCTCAGTGAAGCCTAAATATATGTAATTAAATGATTGGTTAGGTCTTTAGGATTGTGTGAAGTATGGACATAATGGAGAGGAAACTTTTTGGTTTCAGCGGTATGTTTAAGCGTGGCTTCAGCCAATGCTTAGATGTTGGAACACTTAGTCGGCAGAGAGATAGACGCTTAAATGTGGGTCCTAGTTCCTATGTGGAAAGTGGGAGGTTTGATTGAAATAACTGATAAATTCATGAAAAGGTAATGCAAGGTGAACTTGAGGAATTGTCTTAGTTGTTTTAGCTATCCGAGTTCATATATTTGGTTAGTATTAGTACTTCAAAATTATTTATTCTTCAAATGTACCTAGTCTATATTGGGCCACTTGCTTGAGATCATAAGCAAAACTTTAATGCTGACTTGATTGACATGAAAGCTAATTCAGAAATCCTTAATTTATGTTTTTTATCAACATAATTTCTTAAAGCTCGTTTTCAGTAAAATCTTACTTTTTTATTTTTTTAAAACTAGAGTCTTTCGGATTATTTTCTTTGTATTACCTTCAAATTAAAAGTATGTGGCTGATGTTCTTTATCATAATTATAATATTTTTAGTATGTGAAGTAGGTTTTTATTCTATGATATTTTCATGATATGAGCCTAAATTAGTTCCTTCAGATTTGGTTGCTGGATTAAGTTGCAGGTTCTTTGGAATAAGTTTCACAGGAATATGAACAGTGTTTAAATTTAAACTTTTCTTTTGATTTGACTTGGGAATGATTTGTTATACCTCAATCAACTTTCTTCTCCTTTGCTTTTCGTTGCTGGAGACATATGGGGGAATTTGGAAGATGTGTGTGTGATTATAGAGGGGTTTGAGATCTTCATTCTGAAAGTATGTTGAGCTGCTCACTGTTATTTGTTTTCACTGTTTCAGCTCCAGCTACTACGACGATGAATCTGATGAAGATGAAGTGAGTAAAATACTTACACTTTACATAATAGGATGAGTTTTTTTTTTTTTTTTTTTTTTTAAATAATAACAGGACAAGTTATTAGTGGCTGTTTCATTGTTAGGTGGAACACATTGAGTGCCTTGGTCTAACGTTCACTGTTGTTTTCAGGCTTCAAATTCTCTGGTGCTTCCTGCTGCAGGTAAGAATGGACATGCTCCTGACTCATTAATTATTTTCTCATTCTCTCCTTATTGTCTTTGCTTTTTATGAAGGATTTGAGTTATAGTGAACCTATTATGCTATTTCCTTTAATCTATATGAGATGAGATTTTGTGGATTCTAAAGCATGAGAAATTGAACTTTCTAATCTCAGATGCACTTTGA
Protein sequence

>MS.gene003455.t1

MHKSNRVEKLCVQTFESVVDVNKVSSLIDSVVKHKVQDLELSLGDRNDEFVLPHSFSSFESLTKLCLGLKFTLHIPSGIRFPSLKKLVVSEVTFADEMSVLQLFSGCPVLQELTLYNCYWENIEQITVAISTLTKLTIDFELFCVGYDHDMTLTIDAVNLLSLSCRCIQTIEFIPVNLTSVVDASIDLEYDHPSSESYAAHCVFELLSGLSSVKSLTLYNNTLECLYYTKDTLHLLPTFNNLTHLEVFAGIPETTNEVLMDILRKAPKVEVLEIPGVVLNYLDGEDLILNAVPCCFKSSLNRLCFLNFYGNEYEIQFVKFILKNSPYLGEIKIHCSRHLLADNEKVDDIWNQLDDVGGPDCVIDFFSSYYDDESDEDEASNSLVLPAADAL