AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene045437

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene045437.t1 MTR_4g080787 93.258 356 23 1 1 356 137 491 0.0 674
MS.gene045437.t1 MTR_4g080773 77.500 360 77 2 1 357 137 495 0.0 513
MS.gene045437.t1 MTR_4g080773 77.437 359 77 2 1 356 137 494 2.41e-180 509
MS.gene045437.t1 MTR_3g048290 67.147 347 106 3 1 340 131 476 6.06e-150 432
MS.gene045437.t1 MTR_4g080807 61.231 325 88 3 1 322 36 325 3.87e-127 371
MS.gene045437.t1 MTR_4g080590 48.204 334 164 4 4 333 128 456 4.57e-87 270
MS.gene045437.t1 MTR_7g023310 49.699 332 154 5 4 328 131 456 3.33e-76 242
MS.gene045437.t1 MTR_4g130200 44.109 331 144 4 4 328 96 391 3.79e-72 229
MS.gene045437.t1 MTR_5g039950 44.688 320 142 6 15 328 129 419 4.81e-70 225
MS.gene045437.t1 MTR_7g023330 49.057 265 122 5 71 328 137 395 2.38e-62 204
MS.gene045437.t1 MTR_7g039630 49.573 234 111 4 15 242 111 343 1.24e-58 193
MS.gene045437.t1 MTR_4g080590 49.160 238 112 4 4 237 128 360 1.97e-57 191
MS.gene045437.t1 MTR_5g039970 54.054 185 79 3 14 192 138 322 7.95e-47 162
MS.gene045437.t1 MTR_4g081030 44.672 244 126 4 1 237 114 355 5.65e-45 159
MS.gene045437.t1 MTR_2g094750 43.657 268 135 8 1 254 211 476 1.18e-41 152
MS.gene045437.t1 MTR_2g094450 37.788 217 100 9 48 249 3 199 3.50e-20 87.8
MS.gene045437.t1 MTR_8g062270 30.314 287 172 11 38 311 170 441 3.84e-16 79.7
MS.gene045437.t1 MTR_1g082260 44.706 85 47 0 14 98 91 175 5.36e-14 70.1
MS.gene045437.t1 MTR_5g084070 28.481 316 192 8 14 320 123 413 2.57e-13 71.2
MS.gene045437.t1 MTR_2g072530 24.776 335 231 8 5 328 121 445 4.73e-13 70.5
MS.gene045437.t1 MTR_5g005690 24.398 332 214 4 1 297 123 452 8.13e-13 69.7
MS.gene045437.t1 MTR_8g467800 26.241 282 172 9 34 296 159 423 1.41e-12 68.9
MS.gene045437.t1 MTR_5g080710 25.773 291 194 8 14 297 146 421 1.25e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene045437.t1 AT1G78750 29.431 299 185 8 12 296 140 426 2.56e-18 86.3
MS.gene045437.t1 AT1G78750 29.431 299 185 8 12 296 140 426 2.56e-18 86.3
MS.gene045437.t1 AT1G66290 29.210 291 184 8 25 303 151 431 9.83e-18 84.7
MS.gene045437.t1 AT5G56390 27.010 311 197 10 7 307 113 403 8.87e-17 81.6
MS.gene045437.t1 AT5G53840 24.036 337 243 7 1 333 117 444 2.32e-16 80.5
MS.gene045437.t1 AT1G32375 26.936 297 182 9 12 296 117 390 3.08e-16 80.1
MS.gene045437.t1 AT1G32375 26.936 297 182 9 12 296 117 390 3.08e-16 80.1
MS.gene045437.t1 AT3G59160 27.302 315 177 13 20 309 157 444 7.96e-16 79.0
MS.gene045437.t1 AT3G52680 26.087 345 206 9 15 337 138 455 1.35e-15 78.2
MS.gene045437.t1 AT3G50710 31.457 302 165 13 17 302 124 399 3.10e-15 77.0
MS.gene045437.t1 AT4G14096 26.331 338 216 15 14 330 126 451 3.12e-15 77.0
MS.gene045437.t1 AT4G14096 26.331 338 216 15 14 330 126 451 3.12e-15 77.0
MS.gene045437.t1 AT3G59200 25.157 318 193 10 43 327 157 462 3.21e-15 77.0
MS.gene045437.t1 AT1G58310 26.975 367 224 11 10 339 139 498 3.48e-15 77.0
MS.gene045437.t1 AT1G66310 28.947 266 160 8 46 297 164 414 9.45e-15 75.5
MS.gene045437.t1 AT3G60040 27.619 315 205 12 4 314 136 431 1.24e-14 75.5
MS.gene045437.t1 AT3G60040 27.619 315 205 12 4 314 353 648 1.29e-14 75.5
MS.gene045437.t1 AT3G60040 27.619 315 205 12 4 314 355 650 1.56e-14 75.5
MS.gene045437.t1 AT4G14103 30.000 240 137 11 14 235 126 352 1.57e-14 74.7
MS.gene045437.t1 AT4G14103 30.000 240 137 11 14 235 126 352 1.57e-14 74.7
MS.gene045437.t1 AT3G60040 27.619 315 205 12 4 314 356 651 1.64e-14 75.5
MS.gene045437.t1 AT3G60040 27.619 315 205 12 4 314 355 650 1.73e-14 75.5
MS.gene045437.t1 AT4G22280 30.389 283 167 13 25 299 125 385 2.06e-14 74.3
MS.gene045437.t1 AT4G14103 30.000 240 137 11 14 235 126 352 2.30e-14 74.3
MS.gene045437.t1 AT5G38590 28.814 295 167 9 20 300 122 387 2.47e-14 74.3
MS.gene045437.t1 AT4G22280 32.569 218 130 9 25 237 125 330 6.44e-14 72.4
MS.gene045437.t1 AT5G22730 27.832 309 197 11 12 301 140 441 9.15e-14 72.8
MS.gene045437.t1 AT5G22730 27.832 309 197 11 12 301 140 441 9.15e-14 72.8
MS.gene045437.t1 AT5G60610 27.517 298 186 9 10 296 108 386 1.55e-13 71.6
MS.gene045437.t1 AT5G60610 27.517 298 186 9 10 296 108 386 1.55e-13 71.6
MS.gene045437.t1 AT3G59200 29.954 217 116 8 43 235 157 361 3.75e-13 70.5
MS.gene045437.t1 AT1G78730 28.507 221 134 6 31 237 133 343 6.10e-13 70.1
MS.gene045437.t1 AT3G59210 26.211 351 217 12 11 330 124 463 7.30e-13 70.1
MS.gene045437.t1 AT3G59210 26.211 351 217 12 11 330 124 463 7.30e-13 70.1
MS.gene045437.t1 AT3G59210 26.211 351 217 12 11 330 124 463 7.30e-13 70.1
MS.gene045437.t1 AT3G59210 26.211 351 217 12 11 330 124 463 7.30e-13 70.1
MS.gene045437.t1 AT3G59210 26.211 351 217 12 11 330 124 463 7.30e-13 70.1
MS.gene045437.t1 AT5G56430 25.000 324 199 8 19 316 50 355 7.35e-13 69.3
MS.gene045437.t1 AT2G42720 27.511 229 127 8 26 235 136 344 8.85e-13 69.7
MS.gene045437.t1 AT2G26860 26.962 293 181 9 17 296 124 396 1.43e-12 68.9
MS.gene045437.t1 AT2G26860 26.962 293 181 9 17 296 124 396 1.43e-12 68.9
MS.gene045437.t1 AT1G66300 27.037 270 180 5 38 297 166 428 1.78e-12 68.6
MS.gene045437.t1 AT1G78760 26.936 297 190 9 12 296 130 411 3.48e-12 67.8
MS.gene045437.t1 AT2G29930 30.233 215 122 10 41 241 31 231 3.69e-12 67.0
MS.gene045437.t1 AT3G59180 25.469 373 217 14 4 327 85 445 5.05e-12 67.4
MS.gene045437.t1 AT1G66320 25.000 308 208 7 17 309 143 442 5.43e-12 67.0
MS.gene045437.t1 AT4G10400 24.926 337 212 11 1 328 104 408 7.10e-12 66.6
MS.gene045437.t1 AT4G10400 24.926 337 212 11 1 328 104 408 7.10e-12 66.6
MS.gene045437.t1 AT4G10400 24.926 337 212 11 1 328 104 408 7.10e-12 66.6
MS.gene045437.t1 AT3G58960 29.600 250 139 14 12 237 115 351 9.16e-12 66.6
MS.gene045437.t1 AT2G29930 30.233 215 122 10 41 241 151 351 9.63e-12 66.2
MS.gene045437.t1 AT2G29930 30.233 215 122 10 41 241 151 351 9.63e-12 66.2
MS.gene045437.t1 AT3G59190 27.948 229 135 10 35 242 157 376 1.24e-11 65.9
MS.gene045437.t1 AT4G00160 26.627 338 216 11 15 338 134 453 1.36e-11 65.9
MS.gene045437.t1 AT1G05080 25.574 305 186 12 17 302 132 414 1.40e-11 65.9
MS.gene045437.t1 AT4G10420 23.856 306 197 8 16 311 27 306 1.68e-11 65.1
MS.gene045437.t1 AT3G62440 25.588 340 203 13 10 315 127 450 1.74e-11 65.5
MS.gene045437.t1 AT2G04230 25.649 308 207 6 21 321 141 433 2.68e-11 65.1
MS.gene045437.t1 AT1G16930 25.163 306 194 10 17 299 133 426 3.10e-11 64.7
MS.gene045437.t1 AT1G16930 25.163 306 194 10 17 299 133 426 3.10e-11 64.7
MS.gene045437.t1 AT1G16930 25.163 306 194 10 17 299 133 426 3.10e-11 64.7
MS.gene045437.t1 AT3G52690 25.974 308 190 12 24 319 2 283 3.48e-11 63.9
MS.gene045437.t1 AT3G59190 27.727 220 131 9 35 235 157 367 4.48e-11 64.3
MS.gene045437.t1 AT4G13965 26.068 234 153 5 5 237 113 327 4.97e-11 64.3
MS.gene045437.t1 AT1G56610 27.586 203 131 5 45 235 121 319 7.77e-11 63.5
MS.gene045437.t1 AT1G56610 27.586 203 131 5 45 235 223 421 7.91e-11 63.9
MS.gene045437.t1 AT3G58860 23.810 294 201 10 13 296 128 408 8.55e-11 63.5
MS.gene045437.t1 AT1G56610 27.586 203 131 5 45 235 160 358 9.70e-11 63.5

Find 61 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACATCTTAATGTGTATTCT+TGG 0.222438 4.2:+29070453 MS.gene045437:CDS
TGTATCACATGAACATATTA+AGG 0.248579 4.2:-29070058 None:intergenic
CCTTCGAAAGACTCCTAAAT+TGG 0.253380 4.2:+29070514 MS.gene045437:CDS
ATCTCAAGAACTTCCAATTT+AGG 0.259052 4.2:-29070527 None:intergenic
ATATTGATCTCCTCTAAATA+TGG 0.270803 4.2:-29070803 None:intergenic
TTAAGATAAACGTAACAAAT+TGG 0.282322 4.2:-29070772 None:intergenic
ACTATGGAGGTCAGGTTAAC+AGG 0.288881 4.2:-29070096 None:intergenic
ATCAATATCTGCATCAACTA+TGG 0.292078 4.2:-29070112 None:intergenic
GAATCCTCTGATGATGAATT+TGG 0.331440 4.2:+29072127 MS.gene045437:CDS
ACATTGCAATATCCACATTA+AGG 0.339640 4.2:+29069953 MS.gene045437:CDS
AAGAGTTGACTTTGTATGAT+TGG 0.347265 4.2:+29069905 MS.gene045437:CDS
TGACTTTGTATGATTGGTCT+TGG 0.365610 4.2:+29069911 MS.gene045437:CDS
GGTAACACAAACTTATCATT+TGG 0.367428 4.2:-29069709 None:intergenic
TACTTCATTAGTGTTGTTCT+CGG 0.386109 4.2:-29070480 None:intergenic
AAAGTTGTGTCATCAACTTT+TGG 0.390132 4.2:+29070903 MS.gene045437:CDS
TATTGATCTCCTCTAAATAT+GGG 0.393682 4.2:-29070802 None:intergenic
TACATCTTCCAATTGGTTGC+GGG 0.408638 4.2:-29070873 None:intergenic
TATTTAGGTACTTAACTACT+TGG 0.425971 4.2:+29070652 MS.gene045437:intron
CCAAGCATACATCTTCCAAT+TGG 0.432178 4.2:-29070880 None:intergenic
TCATTCAATGACTCGAAAGC+TGG 0.433465 4.2:-29069742 None:intergenic
GGATGATGCCCGCAACCAAT+TGG 0.437197 4.2:+29070865 MS.gene045437:CDS
TATTCATTCTTACCTGCAGC+AGG 0.452723 4.2:-29072596 None:intergenic
GACTTTGTATGATTGGTCTT+GGG 0.455661 4.2:+29069912 MS.gene045437:CDS
CAATATCTTAAACTTTCACT+AGG 0.471064 4.2:+29069682 MS.gene045437:CDS
TGGAAGTTCTTGAGATTCCT+CGG 0.471372 4.2:+29070534 MS.gene045437:CDS
GAATATGTAGAGTGAATTTG+AGG 0.474133 4.2:-29069777 None:intergenic
GATGGAGAGTATCCTTTGTA+TGG 0.479906 4.2:-29070406 None:intergenic
CTGCATCAACTATGGAGGTC+AGG 0.483598 4.2:-29070104 None:intergenic
TCTGAGGTGCTCCCTGCTGC+AGG 0.489352 4.2:+29072584 MS.gene045437:CDS
TGAGAAGCAAATACATCATC+AGG 0.490334 4.2:-29070144 None:intergenic
GGATTCATCATCATAGTAGC+TGG 0.491226 4.2:-29072110 None:intergenic
ACTTCATTAGTGTTGTTCTC+GGG 0.497838 4.2:-29070479 None:intergenic
AAAGTCAACTCTTGTAAGAC+TGG 0.499080 4.2:-29069895 None:intergenic
TGTGTCAAATTGTCAAATGA+AGG 0.499334 4.2:-29070434 None:intergenic
ATACATCTTCCAATTGGTTG+CGG 0.507493 4.2:-29070874 None:intergenic
CCAATTGGAAGATGTATGCT+TGG 0.508533 4.2:+29070880 MS.gene045437:CDS
AACTGACTTCTCATTTGCAA+AGG 0.521307 4.2:-29069851 None:intergenic
CATTCAATGACTCGAAAGCT+GGG 0.526527 4.2:-29069741 None:intergenic
ATCAGCTGATATGAAGAAGC+TGG 0.532035 4.2:+29070844 MS.gene045437:CDS
GGAAGTTCTTGAGATTCCTC+GGG 0.541852 4.2:+29070535 MS.gene045437:CDS
TGTCAAATGAAGGAAGGAGA+TGG 0.547136 4.2:-29070424 None:intergenic
CTATGGAGGTCAGGTTAACA+GGG 0.548080 4.2:-29070095 None:intergenic
CTACACAATCAAAGTCAAAA+CGG 0.548472 4.2:-29069987 None:intergenic
CCAATTTAGGAGTCTTTCGA+AGG 0.549079 4.2:-29070514 None:intergenic
TTCACCAAATTCATCATCAG+AGG 0.572464 4.2:-29072131 None:intergenic
TCAAATTGTCAAATGAAGGA+AGG 0.579316 4.2:-29070430 None:intergenic
AGTTCTTGAGATTCCTCGGG+TGG 0.599346 4.2:+29070538 MS.gene045437:CDS
AGAGAAGATTTGAAACAGCA+AGG 0.601972 4.2:-29070704 None:intergenic
CTAGTGTCTTTACCATACAA+AGG 0.611766 4.2:+29070394 MS.gene045437:intron
GACGGTCAACTTCCTTAATG+TGG 0.613042 4.2:-29069965 None:intergenic
ACTAGCTAATGATACTCTTG+AGG 0.617745 4.2:+29070215 MS.gene045437:CDS
TCGAAAGCTGGGAAACTATG+TGG 0.621196 4.2:-29069730 None:intergenic
AAAATAAAACGTACCACCCG+AGG 0.625829 4.2:-29070551 None:intergenic
ATTGATCTCCTCTAAATATG+GGG 0.634717 4.2:-29070801 None:intergenic
ATTCATTCTTACCTGCAGCA+GGG 0.641108 4.2:-29072595 None:intergenic
AATATCTGCATCAACTATGG+AGG 0.641408 4.2:-29070109 None:intergenic
TTAGGGAAATGAATATCACT+AGG 0.642175 4.2:-29069799 None:intergenic
ACAATCAAAGTCAAAACGGA+CGG 0.657130 4.2:-29069983 None:intergenic
TTGTACATTCAAGATTGATG+CGG 0.661658 4.2:+29070023 MS.gene045437:CDS
GAAGCAAATACATCATCAGG+TGG 0.666203 4.2:-29070141 None:intergenic
TGTGCAGTTGAGCTACTGAG+TGG 0.722939 4.2:+29070168 MS.gene045437:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATGCTTTTTAAATTTTTA+TGG + chr4.2:29070734-29070753 MS.gene045437:CDS 10.0%
!!! AAGTGAATCAATTTTTTTAT+TGG + chr4.2:29071056-29071075 MS.gene045437:intron 15.0%
!!! ATATTTTTAGTATGTAAAGT+GGG + chr4.2:29071778-29071797 MS.gene045437:intron 15.0%
!!! TATATTTTTAGTATGTAAAG+TGG + chr4.2:29071777-29071796 MS.gene045437:intron 15.0%
!!! TGTATTTGAGTTAAAATATT+TGG + chr4.2:29071490-29071509 MS.gene045437:intron 15.0%
!! ATTTAACATGTTCATATTCA+TGG - chr4.2:29071904-29071923 None:intergenic 20.0%
!! CATAAAAAGAATAAGCAATA+AGG - chr4.2:29072663-29072682 None:intergenic 20.0%
!! TTAAGATAAACGTAACAAAT+TGG - chr4.2:29070775-29070794 None:intergenic 20.0%
!!! ACTAAAAACAACATTTTCAT+AGG - chr4.2:29070307-29070326 None:intergenic 20.0%
!!! ACTTTTCTTTTGATTTTACT+TGG + chr4.2:29071928-29071947 MS.gene045437:intron 20.0%
!!! AGTTTTACAAAATTCAAAGT+AGG - chr4.2:29071229-29071248 None:intergenic 20.0%
!!! ATTGCTTATTCTTTTTATGA+AGG + chr4.2:29072664-29072683 MS.gene045437:intron 20.0%
!!! CTTTTCTTTTGATTTTACTT+GGG + chr4.2:29071929-29071948 MS.gene045437:intron 20.0%
!!! TACTTTGAATTTTGTAAAAC+TGG + chr4.2:29071228-29071247 MS.gene045437:intron 20.0%
!!! TTGTTTAATGGTTCTTATTT+AGG + chr4.2:29070637-29070656 MS.gene045437:intron 20.0%
! AAAACAGAAATGAAGATGAT+AGG - chr4.2:29070257-29070276 None:intergenic 25.0%
! AAAGAATAAGCAATAAGGAA+AGG - chr4.2:29072658-29072677 None:intergenic 25.0%
! AAATCTGAAGGAACTAAATT+AGG - chr4.2:29071834-29071853 None:intergenic 25.0%
! ATATTGATCTCCTCTAAATA+TGG - chr4.2:29070806-29070825 None:intergenic 25.0%
! CAATATCTTAAACTTTCACT+AGG + chr4.2:29069682-29069701 MS.gene045437:CDS 25.0%
! TATTGATCTCCTCTAAATAT+GGG - chr4.2:29070805-29070824 None:intergenic 25.0%
! TATTTAGGTACTTAACTACT+TGG + chr4.2:29070652-29070671 MS.gene045437:intron 25.0%
! TCCTTCATATTAATAGGATT+TGG + chr4.2:29071740-29071759 MS.gene045437:intron 25.0%
! TGTATCACATGAACATATTA+AGG - chr4.2:29070061-29070080 None:intergenic 25.0%
! TGTGTTTCCTTCATATTAAT+AGG + chr4.2:29071734-29071753 MS.gene045437:intron 25.0%
!! AAATTGATTCACTTCACAAA+TGG - chr4.2:29071050-29071069 None:intergenic 25.0%
!! ACAAGTTCAAGATTCTTTTT+GGG - chr4.2:29071179-29071198 None:intergenic 25.0%
!! ATGTTTGTGTTATTATAGAG+TGG + chr4.2:29072024-29072043 MS.gene045437:intron 25.0%
!! ATTGCATATTTTCTTTCTCT+CGG + chr4.2:29070345-29070364 MS.gene045437:intron 25.0%
!! TAATTTAGTTCCTTCAGATT+TGG + chr4.2:29071833-29071852 MS.gene045437:intron 25.0%
!! TATAACATCCCAACATTTTT+AGG + chr4.2:29071282-29071301 MS.gene045437:intron 25.0%
!! TGTTTGTGTTATTATAGAGT+GGG + chr4.2:29072025-29072044 MS.gene045437:intron 25.0%
!!! ACTGCTAATTTTTTTAGCTT+AGG - chr4.2:29069820-29069839 None:intergenic 25.0%
!!! AGACTACTATGATTAGTTTA+TGG + chr4.2:29071018-29071037 MS.gene045437:intron 25.0%
!!! ATTATTGTTAGTCTTGAACA+TGG - chr4.2:29070598-29070617 None:intergenic 25.0%
!!! CTGCTAATTTTTTTAGCTTA+GGG - chr4.2:29069819-29069838 None:intergenic 25.0%
!!! GTATTTGTTTTCATTGTTTC+AGG + chr4.2:29072086-29072105 MS.gene045437:intron 25.0%
!!! TTGTCAATTGACTTGTTTAA+TGG + chr4.2:29070625-29070644 MS.gene045437:intron 25.0%
AAAAAATGCCCCATATTTAG+AGG + chr4.2:29070793-29070812 MS.gene045437:CDS 30.0%
AACTATGTCATGCTTTAGTA+TGG - chr4.2:29071151-29071170 None:intergenic 30.0%
ACATTGCAATATCCACATTA+AGG + chr4.2:29069953-29069972 MS.gene045437:CDS 30.0%
ACTTCATTGCATAGTACAAA+AGG + chr4.2:29070964-29070983 MS.gene045437:intron 30.0%
ATCAATATCTGCATCAACTA+TGG - chr4.2:29070115-29070134 None:intergenic 30.0%
ATCTCAAGAACTTCCAATTT+AGG - chr4.2:29070530-29070549 None:intergenic 30.0%
ATTGATCTCCTCTAAATATG+GGG - chr4.2:29070804-29070823 None:intergenic 30.0%
CACATCTTAATGTGTATTCT+TGG + chr4.2:29070453-29070472 MS.gene045437:CDS 30.0%
CTACACAATCAAAGTCAAAA+CGG - chr4.2:29069990-29070009 None:intergenic 30.0%
CTTCATTGCATAGTACAAAA+GGG + chr4.2:29070965-29070984 MS.gene045437:intron 30.0%
GAATATGTAGAGTGAATTTG+AGG - chr4.2:29069780-29069799 None:intergenic 30.0%
GAGAGAAAGAAAATATGCAA+TGG - chr4.2:29070346-29070365 None:intergenic 30.0%
GCCAAATCCTATTAATATGA+AGG - chr4.2:29071744-29071763 None:intergenic 30.0%
GGTAACACAAACTTATCATT+TGG - chr4.2:29069712-29069731 None:intergenic 30.0%
TAAAACCTCCTAGTTATTAG+TGG + chr4.2:29072165-29072184 MS.gene045437:intron 30.0%
TAAATGCACCAAGTCTATAT+TGG + chr4.2:29071540-29071559 MS.gene045437:intron 30.0%
TAGCTAATAACTTGATGTGA+AGG + chr4.2:29071427-29071446 MS.gene045437:intron 30.0%
TATGGTAAAGACACTAGTAA+TGG - chr4.2:29070391-29070410 None:intergenic 30.0%
TCAAATTGTCAAATGAAGGA+AGG - chr4.2:29070433-29070452 None:intergenic 30.0%
TGTGTCAAATTGTCAAATGA+AGG - chr4.2:29070437-29070456 None:intergenic 30.0%
TTAGGGAAATGAATATCACT+AGG - chr4.2:29069802-29069821 None:intergenic 30.0%
TTGTACATTCAAGATTGATG+CGG + chr4.2:29070023-29070042 MS.gene045437:CDS 30.0%
! AAAGTTGTGTCATCAACTTT+TGG + chr4.2:29070903-29070922 MS.gene045437:CDS 30.0%
! AAGAGTTGACTTTGTATGAT+TGG + chr4.2:29069905-29069924 MS.gene045437:CDS 30.0%
! CAAGTTCAAGATTCTTTTTG+GGG - chr4.2:29071178-29071197 None:intergenic 30.0%
! GACAAGTTCAAGATTCTTTT+TGG - chr4.2:29071180-29071199 None:intergenic 30.0%
! GTTTGTGTTATTATAGAGTG+GGG + chr4.2:29072026-29072045 MS.gene045437:intron 30.0%
! TACTTCATTAGTGTTGTTCT+CGG - chr4.2:29070483-29070502 None:intergenic 30.0%
!! AAATAAGACAAAGGCATTCT+AGG - chr4.2:29072539-29072558 None:intergenic 30.0%
!! TTATTTTCGTCGTTGTTTTC+AGG + chr4.2:29072553-29072572 MS.gene045437:intron 30.0%
AAAACTTTCACTTTCCGTAC+AGG - chr4.2:29071399-29071418 None:intergenic 35.0%
AAAGCAGTCCAATATAGACT+TGG - chr4.2:29071551-29071570 None:intergenic 35.0%
AAAGTCAACTCTTGTAAGAC+TGG - chr4.2:29069898-29069917 None:intergenic 35.0%
AAGCAGCTCAGAATAATTTC+AGG - chr4.2:29072066-29072085 None:intergenic 35.0%
AATATCTGCATCAACTATGG+AGG - chr4.2:29070112-29070131 None:intergenic 35.0%
AATCCAACAACCAAATCTGA+AGG - chr4.2:29071846-29071865 None:intergenic 35.0%
ACAATCAAAGTCAAAACGGA+CGG - chr4.2:29069986-29070005 None:intergenic 35.0%
ACAGCTGAACCTAAAAATGT+TGG - chr4.2:29071294-29071313 None:intergenic 35.0%
ACTAGCTAATGATACTCTTG+AGG + chr4.2:29070215-29070234 MS.gene045437:CDS 35.0%
AGAGATAGACACTTAAACGT+GGG + chr4.2:29071365-29071384 MS.gene045437:intron 35.0%
AGCAGCTCAGAATAATTTCA+GGG - chr4.2:29072065-29072084 None:intergenic 35.0%
AGCGAAGGAAAAGAAAGTTA+AGG - chr4.2:29071969-29071988 None:intergenic 35.0%
ATACATCTTCCAATTGGTTG+CGG - chr4.2:29070877-29070896 None:intergenic 35.0%
CAACGACGAAAATAAGACAA+AGG - chr4.2:29072548-29072567 None:intergenic 35.0%
CAGCTGAACCTAAAAATGTT+GGG - chr4.2:29071293-29071312 None:intergenic 35.0%
CTAGTGTCTTTACCATACAA+AGG + chr4.2:29070394-29070413 MS.gene045437:intron 35.0%
CTTGTCTTTCAAACTTCAGT+AGG + chr4.2:29071194-29071213 MS.gene045437:intron 35.0%
TAAGTGCAAAATCGAAGCAT+TGG - chr4.2:29071337-29071356 None:intergenic 35.0%
TCATTGTTAGGTGGAACATT+TGG + chr4.2:29072193-29072212 MS.gene045437:intron 35.0%
TGAAACAGCCACTAATAACT+AGG - chr4.2:29072176-29072195 None:intergenic 35.0%
TGAGAAGCAAATACATCATC+AGG - chr4.2:29070147-29070166 None:intergenic 35.0%
TTCACCAAATTCATCATCAG+AGG - chr4.2:29072134-29072153 None:intergenic 35.0%
! AACTGACTTCTCATTTGCAA+AGG - chr4.2:29069854-29069873 None:intergenic 35.0%
! ACTTCATTAGTGTTGTTCTC+GGG - chr4.2:29070482-29070501 None:intergenic 35.0%
! AGAGAAGATTTGAAACAGCA+AGG - chr4.2:29070707-29070726 None:intergenic 35.0%
! GAATCCTCTGATGATGAATT+TGG + chr4.2:29072127-29072146 MS.gene045437:CDS 35.0%
! GCTTCGATTTTGCACTTAAT+TGG + chr4.2:29071339-29071358 MS.gene045437:intron 35.0%
! TTAGTGGCTGTTTCATTGTT+AGG + chr4.2:29072181-29072200 MS.gene045437:intron 35.0%
!! GACTTTGTATGATTGGTCTT+GGG + chr4.2:29069912-29069931 MS.gene045437:CDS 35.0%
!! TGACTTTGTATGATTGGTCT+TGG + chr4.2:29069911-29069930 MS.gene045437:CDS 35.0%
!! TTTGTTGTTGGGAACGTATT+GGG + chr4.2:29071990-29072009 MS.gene045437:intron 35.0%
!!! CAGTTCAACGACTTTTTTCT+GGG + chr4.2:29069869-29069888 MS.gene045437:CDS 35.0%
!!! TCAGTTCAACGACTTTTTTC+TGG + chr4.2:29069868-29069887 MS.gene045437:CDS 35.0%
!!! TTCCTTCGCTTTTTGTTGTT+GGG + chr4.2:29071979-29071998 MS.gene045437:intron 35.0%
!!! TTTCCTTCGCTTTTTGTTGT+TGG + chr4.2:29071978-29071997 MS.gene045437:intron 35.0%
!!! TTTTGTTGTTGGGAACGTAT+TGG + chr4.2:29071989-29072008 MS.gene045437:intron 35.0%
AAAATAAAACGTACCACCCG+AGG - chr4.2:29070554-29070573 None:intergenic 40.0%
AACAGCCACTAATAACTAGG+AGG - chr4.2:29072173-29072192 None:intergenic 40.0%
AAGCAGCTCAGCATAATTTC+AGG - chr4.2:29072391-29072410 None:intergenic 40.0%
AGCAGCTCAGCATAATTTCA+GGG - chr4.2:29072390-29072409 None:intergenic 40.0%
ATCAGCTGATATGAAGAAGC+TGG + chr4.2:29070844-29070863 MS.gene045437:CDS 40.0%
ATGTTATAGCATGTGAGCGT+AGG - chr4.2:29071270-29071289 None:intergenic 40.0%
ATTCATTCTTACCTGCAGCA+GGG - chr4.2:29072598-29072617 None:intergenic 40.0%
ATTGGACAAACTCTGTTTGC+TGG + chr4.2:29071074-29071093 MS.gene045437:intron 40.0%
CATTCAATGACTCGAAAGCT+GGG - chr4.2:29069744-29069763 None:intergenic 40.0%
CATTGCATAGTACAAAAGGG+TGG + chr4.2:29070968-29070987 MS.gene045437:intron 40.0%
CCAAGCATACATCTTCCAAT+TGG - chr4.2:29070883-29070902 None:intergenic 40.0%
CCAATTGGAAGATGTATGCT+TGG + chr4.2:29070880-29070899 MS.gene045437:CDS 40.0%
CCAATTTAGGAGTCTTTCGA+AGG - chr4.2:29070517-29070536 None:intergenic 40.0%
CCTTCGAAAGACTCCTAAAT+TGG + chr4.2:29070514-29070533 MS.gene045437:CDS 40.0%
GAAGCAAATACATCATCAGG+TGG - chr4.2:29070144-29070163 None:intergenic 40.0%
GAGAGATAGACACTTAAACG+TGG + chr4.2:29071364-29071383 MS.gene045437:intron 40.0%
GGATTAATCTACAGCCTACA+GGG + chr4.2:29071861-29071880 MS.gene045437:intron 40.0%
GGATTCATCATCATAGTAGC+TGG - chr4.2:29072113-29072132 None:intergenic 40.0%
GTCTTTCAAACTTCAGTAGG+TGG + chr4.2:29071197-29071216 MS.gene045437:intron 40.0%
GTTATTATAGAGTGGGGTCT+GGG + chr4.2:29072032-29072051 MS.gene045437:intron 40.0%
TACATCTTCCAATTGGTTGC+GGG - chr4.2:29070876-29070895 None:intergenic 40.0%
TATTCATTCTTACCTGCAGC+AGG - chr4.2:29072599-29072618 None:intergenic 40.0%
TCATTCAATGACTCGAAAGC+TGG - chr4.2:29069745-29069764 None:intergenic 40.0%
TGGAAGTTCTTGAGATTCCT+CGG + chr4.2:29070534-29070553 MS.gene045437:CDS 40.0%
TGGATTAATCTACAGCCTAC+AGG + chr4.2:29071860-29071879 MS.gene045437:intron 40.0%
TGTCAAATGAAGGAAGGAGA+TGG - chr4.2:29070427-29070446 None:intergenic 40.0%
TGTTATAGCATGTGAGCGTA+GGG - chr4.2:29071269-29071288 None:intergenic 40.0%
TGTTATTATAGAGTGGGGTC+TGG + chr4.2:29072031-29072050 MS.gene045437:intron 40.0%
TTCCCAACAACAAAAAGCGA+AGG - chr4.2:29071984-29072003 None:intergenic 40.0%
TTGGACAAACTCTGTTTGCT+GGG + chr4.2:29071075-29071094 MS.gene045437:intron 40.0%
! GGTAGTGCAAGTTGAATTAG+AGG + chr4.2:29071448-29071467 MS.gene045437:intron 40.0%
! GTTCCTTCAGATTTGGTTGT+TGG + chr4.2:29071840-29071859 MS.gene045437:intron 40.0%
! TTGTTGTTGGGAACGTATTG+GGG + chr4.2:29071991-29072010 MS.gene045437:intron 40.0%
!! GATGGAGAGTATCCTTTGTA+TGG - chr4.2:29070409-29070428 None:intergenic 40.0%
!! TTTTCAGGCTGCAAATTCTG+AGG + chr4.2:29072568-29072587 MS.gene045437:intron 40.0%
ACTATGGAGGTCAGGTTAAC+AGG - chr4.2:29070099-29070118 None:intergenic 45.0%
ACTTGTTCCAAACACCCTGT+AGG - chr4.2:29071878-29071897 None:intergenic 45.0%
CTATGGAGGTCAGGTTAACA+GGG - chr4.2:29070098-29070117 None:intergenic 45.0%
GACGGTCAACTTCCTTAATG+TGG - chr4.2:29069968-29069987 None:intergenic 45.0%
GGAAGTTCTTGAGATTCCTC+GGG + chr4.2:29070535-29070554 MS.gene045437:CDS 45.0%
TCACTTTCCGTACAGGAACT+GGG - chr4.2:29071392-29071411 None:intergenic 45.0%
TCGAAAGCTGGGAAACTATG+TGG - chr4.2:29069733-29069752 None:intergenic 45.0%
TTCACTTTCCGTACAGGAAC+TGG - chr4.2:29071393-29071412 None:intergenic 45.0%
TTCAGCTGTACGCTTAAGCA+TGG + chr4.2:29071305-29071324 MS.gene045437:intron 45.0%
! GTGGCTGTTTCATTGTTAGG+TGG + chr4.2:29072184-29072203 MS.gene045437:intron 45.0%
!! TGCAAAATCGAAGCATTGGC+CGG - chr4.2:29071333-29071352 None:intergenic 45.0%
AGTTCTTGAGATTCCTCGGG+TGG + chr4.2:29070538-29070557 MS.gene045437:CDS 50.0%
ATAGCATGTGAGCGTAGGGT+AGG - chr4.2:29071265-29071284 None:intergenic 50.0%
CTGCATCAACTATGGAGGTC+AGG - chr4.2:29070107-29070126 None:intergenic 50.0%
TAGCATGTGAGCGTAGGGTA+GGG - chr4.2:29071264-29071283 None:intergenic 50.0%
TGTACGCTTAAGCATGGCTC+CGG + chr4.2:29071311-29071330 MS.gene045437:intron 50.0%
TGTGCAGTTGAGCTACTGAG+TGG + chr4.2:29070168-29070187 MS.gene045437:CDS 50.0%
! TCTACAGCCTACAGGGTGTT+TGG + chr4.2:29071868-29071887 MS.gene045437:intron 50.0%
! TGGGAACGTATTGGGGCATT+TGG + chr4.2:29071998-29072017 MS.gene045437:intron 50.0%
AGCATGTGAGCGTAGGGTAG+GGG - chr4.2:29071263-29071282 None:intergenic 55.0%
GGATGATGCCCGCAACCAAT+TGG + chr4.2:29070865-29070884 MS.gene045437:CDS 55.0%
CGTGGGTCCCAGTTCCTGTA+CGG + chr4.2:29071382-29071401 MS.gene045437:intron 60.0%
TGTGAGCGTAGGGTAGGGGA+AGG - chr4.2:29071259-29071278 None:intergenic 60.0%
! TCTGAGGTGCTCCCTGCTGC+AGG + chr4.2:29072584-29072603 MS.gene045437:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 29069679 29072699 29069679 ID=MS.gene045437
chr4.2 mRNA 29069679 29072699 29069679 ID=MS.gene045437.t1;Parent=MS.gene045437
chr4.2 exon 29069679 29070236 29069679 ID=MS.gene045437.t1.exon1;Parent=MS.gene045437.t1
chr4.2 CDS 29069679 29070236 29069679 ID=cds.MS.gene045437.t1;Parent=MS.gene045437.t1
chr4.2 exon 29070398 29070559 29070398 ID=MS.gene045437.t1.exon2;Parent=MS.gene045437.t1
chr4.2 CDS 29070398 29070559 29070398 ID=cds.MS.gene045437.t1;Parent=MS.gene045437.t1
chr4.2 exon 29070659 29070924 29070659 ID=MS.gene045437.t1.exon3;Parent=MS.gene045437.t1
chr4.2 CDS 29070659 29070924 29070659 ID=cds.MS.gene045437.t1;Parent=MS.gene045437.t1
chr4.2 exon 29072108 29072159 29072108 ID=MS.gene045437.t1.exon4;Parent=MS.gene045437.t1
chr4.2 CDS 29072108 29072159 29072108 ID=cds.MS.gene045437.t1;Parent=MS.gene045437.t1
chr4.2 exon 29072575 29072605 29072575 ID=MS.gene045437.t1.exon5;Parent=MS.gene045437.t1
chr4.2 CDS 29072575 29072605 29072575 ID=cds.MS.gene045437.t1;Parent=MS.gene045437.t1
chr4.2 exon 29072686 29072699 29072686 ID=MS.gene045437.t1.exon6;Parent=MS.gene045437.t1
chr4.2 CDS 29072686 29072699 29072686 ID=cds.MS.gene045437.t1;Parent=MS.gene045437.t1
Gene Sequence

>MS.gene045437

ATGCAATATCTTAAACTTTCACTAGGTCATCCAAATGATAAGTTTGTGTTACCACATAGTTTCCCAGCTTTCGAGTCATTGAATGAACTGCATTTAAACCTCAAATTCACTCTACATATTCCTAGTGATATTCATTTCCCTAAGCTAAAAAAATTAGCAGTTTCAAATGTTACCTTTGCAAATGAGAAGTCAGTTCAACGACTTTTTTCTGGGTGTCCAGTCTTACAAGAGTTGACTTTGTATGATTGGTCTTGGGAGAACATAATGCACATTAACATTGCAATATCCACATTAAGGAAGTTGACCGTCCGTTTTGACTTTGATTGTGTAGATTATGATCATGATTGTACATTCAAGATTGATGCGGCGAATCTTATATCCTTAATATGTTCATGTGATACAACAATAGAATGTATCCCTGTTAACCTGACCTCCATAGTTGATGCAGATATTGATTTGTTTCCACCTGATGATGTATTTGCTTCTCAGTGTGCAGTTGAGCTACTGAGTGGACTTAGCAGTGTCAAGTCTCTCAAACTAGCTAATGATACTCTTGAGGTATGTTACACTATTTGCCTATCATCTTCATTTCTGTTTTTTTTTTGAAGTATTGCATACAATACTGCCTATGAAAATGTTGTTTTTAGTTATGTGTTTCTTATAACCATTGCATATTTTCTTTCTCTCGGTACTGAACATAGTTTTGTTTCCATTACTAGTGTCTTTACCATACAAAGGATACTCTCCATCTCCTTCCTTCATTTGACAATTTGACACATCTTAATGTGTATTCTTGGAGTCCCGAGAACAACACTAATGAAGTACTGATGCACATCCTTCGAAAGACTCCTAAATTGGAAGTTCTTGAGATTCCTCGGGTGGTACGTTTTATTTTCTGCATCAGTGTTTGTATTTGCCATGTTCAAGACTAACAATAATATCATTTTTGTCAATTGACTTGTTTAATGGTTCTTATTTAGGTACTTAACTACTTGGATAGTGAAGATTTGATATTGAACTCAGTGCCTTGCTGTTTCAAATCTTCTCTCAATCGATTATGCTTTTTAAATTTTTATGGAAATGAATATGAAATCCAATTTGTTACGTTTATCTTAAAAAATGCCCCATATTTAGAGGAGATCAATATACACTGTTCGAGACATTTATCAGCTGATATGAAGAAGCTGGATGATGCCCGCAACCAATTGGAAGATGTATGCTTGGAAAGTTGTGTCATCAACTTTTGGTAAATCTTCTTTCTCACATGCTTCAATTTACTAATTCAACTTCATTGCATAGTACAAAAGGGTGGTTGATGAAATAAATAGCTTCACTAGTTAGACTACTATGATTAGTTTATGGAGTATGCCATTTGTGAAGTGAATCAATTTTTTTATTGGACAAACTCTGTTTGCTGGGAAATGAAACGATCTAAGTGTTTAGAGTGATTGTTCTTACTTTTATACTTGCCATACTAAAGCATGACATAGTTTTTACCCCAAAAAGAATCTTGAACTTGTCTTTCAAACTTCAGTAGGTGGTTTCGTCCTACTTTGAATTTTGTAAAACTGGTTCATCCTTCCCCTACCCTACGCTCACATGCTATAACATCCCAACATTTTTAGGTTCAGCTGTACGCTTAAGCATGGCTCCGGCCAATGCTTCGATTTTGCACTTAATTGGCAGAGAGATAGACACTTAAACGTGGGTCCCAGTTCCTGTACGGAAAGTGAAAGTTTTGTTTGAAATAGCTAATAACTTGATGTGAAGGTAGTGCAAGTTGAATTAGAGGAATTGTATTATTTGTTTTATGTATTTGAGTTAAAATATTTGGTTAGTACTTAAAAGTTATATATTTTTTTAAATGCACCAAGTCTATATTGGACTGCTTTTTGTGATCATAAGCAAAACTTTAATGTTGACTTGATTGACATGAAAGCTAATTCAAAATTCTTCACTGTATTTTCTATATCAACATAATTTCGTAAAGCTCTTTTTCAGTAATATCTTCAATTTTTTATTTCTTGAAACTATAGACTTTTGCATTATTTTCTCTGTGTTTCCTTCATATTAATAGGATTTGGCTGATGTTCTTTGTTATATTTTTAGTATGTAAAGTGGGTTTTTTACTATGATCTTTTCATGATATGAGCCTAATTTAGTTCCTTCAGATTTGGTTGTTGGATTAATCTACAGCCTACAGGGTGTTTGGAACAAGTTTTCCATGAATATGAACATGTTAAATTTAAACTTTTCTTTTGATTTTACTTGGGAATGTTTCGATTTGCCTTAACTTTCTTTTCCTTCGCTTTTTGTTGTTGGGAACGTATTGGGGCATTTGGAAGATGTTTGTGTTATTATAGAGTGGGGTCTGGGATCTTGACCCTGAAATTATTCTGAGCTGCTTGTATTTGTTTTCATTGTTTCAGGTCCAGCTACTATGATGATGAATCCTCTGATGATGAATTTGGTGAAGATGAAGTGAGTAAAACCTCCTAGTTATTAGTGGCTGTTTCATTGTTAGGTGGAACATTTGGAACAAGTTTCTCATGAATAAGAACATGTTAAATTTAAACTTTTCTTTTGATTTTACTTGAGAATGTTTCGATTTGCCTTAACTTTCTTTTCCTTCGCTTTTTGTTGTTGGGAACGTATTGGGGCATTTGGAAGATGTTTGTGTTATTATAGAGTGGGGTCTGGGATCTTGACCCTGAAATTATGCTGAGCTGCTTGTATTTGTTTTCATTGTTTCAGGTCCAGCTACTATGATGATGAATCCTCTGATGATGAATTTGGTGAAGATGAAGTGAGTAAAACCTCCTAGTTATTAGTGGCTGTTTCATTGTTAGGTGGAACACCTAGAATGCCTTTGTCTTATTTTCGTCGTTGTTTTCAGGCTGCAAATTCTGAGGTGCTCCCTGCTGCAGGTAAGAATGAATATGCTTTGACTGATTATTATTTTCACACTGTCTTCATCCTTTCCTTATTGCTTATTCTTTTTATGAAGGACTTGAGTTATAG
Protein sequence

>MS.gene045437.t1

MQYLKLSLGHPNDKFVLPHSFPAFESLNELHLNLKFTLHIPSDIHFPKLKKLAVSNVTFANEKSVQRLFSGCPVLQELTLYDWSWENIMHINIAISTLRKLTVRFDFDCVDYDHDCTFKIDAANLISLICSCDTTIECIPVNLTSIVDADIDLFPPDDVFASQCAVELLSGLSSVKSLKLANDTLECLYHTKDTLHLLPSFDNLTHLNVYSWSPENNTNEVLMHILRKTPKLEVLEIPRVVLNYLDSEDLILNSVPCCFKSSLNRLCFLNFYGNEYEIQFVTFILKNAPYLEEINIHCSRHLSADMKKLDDARNQLEDVCLESCVINFWSSYYDDESSDDEFGEDEAANSEVLPAAGLEL