AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene035630


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035630.t1 MTR_4g130200 88.976 127 14 0 138 264 187 313 3.09e-71 224
MS.gene035630.t1 MTR_4g130200 69.286 140 8 1 1 140 1 105 6.82e-53 177
MS.gene035630.t1 MTR_3g048290 56.115 139 56 3 1 135 1 138 1.62e-34 130
MS.gene035630.t1 MTR_3g048290 41.406 128 71 1 142 265 260 387 3.31e-13 69.7
MS.gene035630.t1 MTR_5g039970 55.652 115 49 2 10 124 9 121 5.64e-32 121
MS.gene035630.t1 MTR_7g023330 45.714 140 73 2 1 140 1 137 3.50e-30 117
MS.gene035630.t1 MTR_7g023330 46.903 113 58 1 142 254 199 309 5.03e-19 86.7
MS.gene035630.t1 MTR_4g080590 49.640 139 61 3 1 136 1 133 4.87e-30 116
MS.gene035630.t1 MTR_4g080590 53.571 112 49 2 141 251 251 360 9.95e-23 96.7
MS.gene035630.t1 MTR_4g080590 49.640 139 61 3 1 136 1 133 2.23e-29 116
MS.gene035630.t1 MTR_4g080590 52.419 124 56 2 141 263 251 372 1.24e-25 105
MS.gene035630.t1 MTR_4g080773 49.306 144 61 3 7 139 6 148 2.50e-29 116
MS.gene035630.t1 MTR_4g080773 49.194 124 63 0 142 265 266 389 2.20e-26 108
MS.gene035630.t1 MTR_4g080773 50.000 140 58 3 7 135 6 144 2.69e-29 116
MS.gene035630.t1 MTR_4g080773 49.194 124 63 0 142 265 266 389 2.34e-26 108
MS.gene035630.t1 MTR_4g080787 49.286 140 59 3 7 135 6 144 2.23e-28 114
MS.gene035630.t1 MTR_4g080787 34.081 223 111 8 68 265 175 386 9.97e-21 92.0
MS.gene035630.t1 MTR_5g039950 45.324 139 54 3 1 136 1 120 4.51e-27 109
MS.gene035630.t1 MTR_5g039950 45.600 125 67 1 141 264 217 341 1.21e-23 99.8
MS.gene035630.t1 MTR_7g023310 41.497 147 74 4 1 141 1 141 5.56e-26 106
MS.gene035630.t1 MTR_7g023310 47.273 110 56 1 142 251 260 367 2.72e-13 70.1
MS.gene035630.t1 MTR_7g039630 48.214 112 57 1 141 251 227 338 2.09e-23 98.2
MS.gene035630.t1 MTR_4g080813 65.672 67 20 1 5 68 1 67 6.79e-22 88.2
MS.gene035630.t1 MTR_4g080807 47.619 126 61 3 130 251 116 240 1.17e-21 94.4
MS.gene035630.t1 MTR_4g081030 36.196 163 84 4 6 166 3 147 2.88e-17 81.3
MS.gene035630.t1 MTR_4g081030 40.517 116 64 1 141 251 240 355 1.98e-12 67.0
MS.gene035630.t1 MTR_2g094750 43.810 105 57 2 10 113 80 183 1.87e-16 79.3
MS.gene035630.t1 MTR_2g094750 40.833 120 66 3 134 249 339 457 6.42e-12 65.9
MS.gene035630.t1 MTR_2g082800 31.206 141 88 4 11 149 18 151 2.78e-13 69.7
MS.gene035630.t1 MTR_8g014290 49.153 59 25 1 19 72 9 67 1.58e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 32 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCTGCTAACTTCAAATTT+TGG 0.166111 8.1:-3165152 None:intergenic
CCCTAGAATTTATAGATTCT+AGG 0.250715 8.1:+3165328 MS.gene035630:CDS
ACCTAGAATCTATAAATTCT+AGG 0.302328 8.1:-3165329 None:intergenic
ACTAACACATAATACTATTC+AGG 0.356633 8.1:+3165860 MS.gene035630:CDS
ATCAATACATGCATCAACTA+TGG 0.366463 8.1:-3165748 None:intergenic
GATAGAAAGGCTCTGCATTA+AGG 0.370933 8.1:+3165218 MS.gene035630:CDS
ATGTGAAGAACTTCCAGTTT+AGG 0.378415 8.1:-3166169 None:intergenic
TCTATGTAAGACTCCTAAAC+TGG 0.395837 8.1:+3166156 MS.gene035630:CDS
CTAGGGAAAGTTTAAGATCA+AGG 0.407527 8.1:-3165311 None:intergenic
TTCGTCAAATTGTTGAATGA+AGG 0.451486 8.1:-3166079 None:intergenic
GATTGCCTCTTCGATCTAGT+TGG 0.468138 8.1:+3165171 MS.gene035630:CDS
CCTAGAATCTATAAATTCTA+GGG 0.498270 8.1:-3165328 None:intergenic
TTAGGGAGATTGCATAGACT+TGG 0.519489 8.1:+3166292 MS.gene035630:intron
GCACAATGAGAAACATATAG+TGG 0.526428 8.1:-3165795 None:intergenic
AAAAGATGACCACAGTTCCT+TGG 0.528129 8.1:-3166055 None:intergenic
ACAAATCTGATCGGATAGAA+AGG 0.542475 8.1:+3165205 MS.gene035630:CDS
CATGCATCAACTATGGAGGT+TGG 0.544111 8.1:-3165741 None:intergenic
AGGCTAGCATAGATCATGGT+TGG 0.551000 8.1:+3164913 None:intergenic
ATATCAAGAAGAACGTCCTC+AGG 0.564920 8.1:-3165003 None:intergenic
GGAAGTTCTTCACATTTCCA+AGG 0.565872 8.1:+3166177 MS.gene035630:CDS
ATATGTTTCTCATTGTGCAA+TGG 0.567231 8.1:+3165800 MS.gene035630:CDS
GACTATTGCACAAATCTGAT+CGG 0.579697 8.1:+3165196 MS.gene035630:CDS
ATGGAGCTATTGAGTGAACT+TGG 0.599700 8.1:+3165819 MS.gene035630:CDS
AGGCTCTGCATTAAGGTGCA+AGG 0.612492 8.1:+3165225 MS.gene035630:CDS
GAATTGATATGTATGATGCA+TGG 0.626104 8.1:+3165247 MS.gene035630:CDS
AGAGAAGATTTGATACAACA+AGG 0.632975 8.1:-3166348 None:intergenic
AATACCCTAAAATCATGTCA+AGG 0.637389 8.1:-3166102 None:intergenic
AATACATGCATCAACTATGG+AGG 0.653836 8.1:-3165745 None:intergenic
GTCTTCCAACTAGATCGAAG+AGG 0.665222 8.1:-3165176 None:intergenic
AGATGTGCAAAGACATAACG+CGG 0.677348 8.1:+3164951 MS.gene035630:CDS
TATGATCAGTGACTTACCTG+AGG 0.732408 8.1:+3164987 MS.gene035630:CDS
GATGTGCAAAGACATAACGC+GGG 0.756677 8.1:+3164952 MS.gene035630:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACAAGAATATATTTTATAGC+TGG - chr8.1:3165717-3165736 None:intergenic 20.0%
!!! GTATATTGATTCTTGTTTTA+GGG + chr8.1:3166275-3166294 MS.gene035630:intron 20.0%
!!! TGTATATTGATTCTTGTTTT+AGG + chr8.1:3166274-3166293 MS.gene035630:intron 20.0%
! AAATGATGTAAGATATACCT+TGG - chr8.1:3166197-3166216 None:intergenic 25.0%
! AACCCATATGCATTTATATA+AGG - chr8.1:3165547-3165566 None:intergenic 25.0%
! AATAATATTAGTTGTGAGAC+AGG - chr8.1:3166237-3166256 None:intergenic 25.0%
! ACCTAGAATCTATAAATTCT+AGG - chr8.1:3165332-3165351 None:intergenic 25.0%
! ACTAACACATAATACTATTC+AGG + chr8.1:3165860-3165879 MS.gene035630:CDS 25.0%
! AGAATCAATATACAAGTCAA+TGG - chr8.1:3166268-3166287 None:intergenic 25.0%
! AGATATAATGTCTTCAGATT+TGG - chr8.1:3165450-3165469 None:intergenic 25.0%
! CCTAGAATCTATAAATTCTA+GGG - chr8.1:3165331-3165350 None:intergenic 25.0%
! CTAGAAAAGACGATTTAAAA+TGG - chr8.1:3165620-3165639 None:intergenic 25.0%
! GACCTTATATAAATGCATAT+GGG + chr8.1:3165542-3165561 MS.gene035630:intron 25.0%
! GATATAATGTCTTCAGATTT+GGG - chr8.1:3165449-3165468 None:intergenic 25.0%
! TCAATTATGACTATACAACT+TGG - chr8.1:3165657-3165676 None:intergenic 25.0%
! TGACCTTATATAAATGCATA+TGG + chr8.1:3165541-3165560 MS.gene035630:intron 25.0%
!! AAAAGACGATTTAAAATGGA+GGG - chr8.1:3165616-3165635 None:intergenic 25.0%
!! AAATCGTCTTTTCTAGATTA+TGG + chr8.1:3165624-3165643 MS.gene035630:intron 25.0%
!! AATAAGTTGTGTTTAGAATC+TGG + chr8.1:3165390-3165409 MS.gene035630:intron 25.0%
!! AATCTGCTAACTTCAAATTT+TGG - chr8.1:3165155-3165174 None:intergenic 25.0%
!!! AAATTCCTCATTTTTGTGAT+GGG + chr8.1:3165121-3165140 MS.gene035630:CDS 25.0%
AATACCCTAAAATCATGTCA+AGG - chr8.1:3166105-3166124 None:intergenic 30.0%
AGAGAAGATTTGATACAACA+AGG - chr8.1:3166351-3166370 None:intergenic 30.0%
AGTGAGAAGATGAGAAATTA+TGG - chr8.1:3165365-3165384 None:intergenic 30.0%
ATATGTTTCTCATTGTGCAA+TGG + chr8.1:3165800-3165819 MS.gene035630:CDS 30.0%
ATCAATACATGCATCAACTA+TGG - chr8.1:3165751-3165770 None:intergenic 30.0%
CCCTAGAATTTATAGATTCT+AGG + chr8.1:3165328-3165347 MS.gene035630:CDS 30.0%
GAATTGATATGTATGATGCA+TGG + chr8.1:3165247-3165266 MS.gene035630:CDS 30.0%
GTGAGAAGATGAGAAATTAT+GGG - chr8.1:3165364-3165383 None:intergenic 30.0%
GTTCATATGTTTATACCTGA+TGG + chr8.1:3165414-3165433 MS.gene035630:intron 30.0%
TATATAAGGTCAACTCTTCA+AGG - chr8.1:3165533-3165552 None:intergenic 30.0%
TGACTATACAACTTGGAAAA+TGG - chr8.1:3165650-3165669 None:intergenic 30.0%
TGAGAAGATGAGAAATTATG+GGG - chr8.1:3165363-3165382 None:intergenic 30.0%
TTCCTTCAATAATGAGATGT+CGG + chr8.1:3165479-3165498 MS.gene035630:intron 30.0%
TTCGTCAAATTGTTGAATGA+AGG - chr8.1:3166082-3166101 None:intergenic 30.0%
! AGAAAAACGCTGGTTAATTA+AGG - chr8.1:3166032-3166051 None:intergenic 30.0%
! GAAAAGACGATTTAAAATGG+AGG - chr8.1:3165617-3165636 None:intergenic 30.0%
!! CAAATTCCTCATTTTTGTGA+TGG + chr8.1:3165120-3165139 MS.gene035630:CDS 30.0%
!! TGCCAATATGGATGTTTTTA+CGG - chr8.1:3165055-3165074 None:intergenic 30.0%
!!! ATATAGTGGATTTTCAGGAT+AGG - chr8.1:3165784-3165803 None:intergenic 30.0%
!!! GAAACATATAGTGGATTTTC+AGG - chr8.1:3165789-3165808 None:intergenic 30.0%
AATACATGCATCAACTATGG+AGG - chr8.1:3165748-3165767 None:intergenic 35.0%
ACAAATCTGATCGGATAGAA+AGG + chr8.1:3165205-3165224 MS.gene035630:CDS 35.0%
ATGTGAAGAACTTCCAGTTT+AGG - chr8.1:3166172-3166191 None:intergenic 35.0%
CATCCATATTGGCAAAAAAG+TGG + chr8.1:3165061-3165080 MS.gene035630:CDS 35.0%
GACTATTGCACAAATCTGAT+CGG + chr8.1:3165196-3165215 MS.gene035630:CDS 35.0%
GCACAATGAGAAACATATAG+TGG - chr8.1:3165798-3165817 None:intergenic 35.0%
GTGTTGCAATTTCCACATTA+AGG + chr8.1:3165583-3165602 MS.gene035630:intron 35.0%
TCTATGTAAGACTCCTAAAC+TGG + chr8.1:3166156-3166175 MS.gene035630:CDS 35.0%
TGCCGTAAAAACATCCATAT+TGG + chr8.1:3165050-3165069 MS.gene035630:CDS 35.0%
TGTAACCCATCACAAAAATG+AGG - chr8.1:3165129-3165148 None:intergenic 35.0%
! ACGAACCTTGACATGATTTT+AGG + chr8.1:3166097-3166116 MS.gene035630:CDS 35.0%
! CGAACCTTGACATGATTTTA+GGG + chr8.1:3166098-3166117 MS.gene035630:CDS 35.0%
! CTAGGGAAAGTTTAAGATCA+AGG - chr8.1:3165314-3165333 None:intergenic 35.0%
! TTTTCTTATCCAAGGAACTG+TGG + chr8.1:3166046-3166065 MS.gene035630:CDS 35.0%
! TTTTTACGGCATCTTTGGTA+GGG - chr8.1:3165041-3165060 None:intergenic 35.0%
AAAAGATGACCACAGTTCCT+TGG - chr8.1:3166058-3166077 None:intergenic 40.0%
AAGGTCAACTCTTCAAGGAT+AGG - chr8.1:3165528-3165547 None:intergenic 40.0%
AGATGTGCAAAGACATAACG+CGG + chr8.1:3164951-3164970 MS.gene035630:CDS 40.0%
AGGTCAACTCTTCAAGGATA+GGG - chr8.1:3165527-3165546 None:intergenic 40.0%
ATATCAAGAAGAACGTCCTC+AGG - chr8.1:3165006-3165025 None:intergenic 40.0%
ATGGAGCTATTGAGTGAACT+TGG + chr8.1:3165819-3165838 MS.gene035630:CDS 40.0%
CCATATTGGCAAAAAAGTGG+AGG + chr8.1:3165064-3165083 MS.gene035630:CDS 40.0%
GAGGGTTAACTTCCTTAATG+TGG - chr8.1:3165598-3165617 None:intergenic 40.0%
GATAGAAAGGCTCTGCATTA+AGG + chr8.1:3165218-3165237 MS.gene035630:CDS 40.0%
GGAAGTTCTTCACATTTCCA+AGG + chr8.1:3166177-3166196 MS.gene035630:CDS 40.0%
TATGATCAGTGACTTACCTG+AGG + chr8.1:3164987-3165006 MS.gene035630:CDS 40.0%
TCCTTGGATAAGAAAAACGC+TGG - chr8.1:3166042-3166061 None:intergenic 40.0%
TTAGGGAGATTGCATAGACT+TGG + chr8.1:3166292-3166311 MS.gene035630:intron 40.0%
TTTGGGAAGCAAATACCATC+AGG - chr8.1:3165432-3165451 None:intergenic 40.0%
! ACCAGCGTTTTTCTTATCCA+AGG + chr8.1:3166038-3166057 MS.gene035630:intron 40.0%
! GTTTTTACGGCATCTTTGGT+AGG - chr8.1:3165042-3165061 None:intergenic 40.0%
!! GGATGTTTTTACGGCATCTT+TGG - chr8.1:3165046-3165065 None:intergenic 40.0%
!!! CCTCCACTTTTTTGCCAATA+TGG - chr8.1:3165067-3165086 None:intergenic 40.0%
CATGCATCAACTATGGAGGT+TGG - chr8.1:3165744-3165763 None:intergenic 45.0%
GATGTGCAAAGACATAACGC+GGG + chr8.1:3164952-3164971 MS.gene035630:CDS 45.0%
GATTGCCTCTTCGATCTAGT+TGG + chr8.1:3165171-3165190 MS.gene035630:CDS 45.0%
GGCCGACATCTCATTATTGA+AGG - chr8.1:3165484-3165503 None:intergenic 45.0%
GTCTTCCAACTAGATCGAAG+AGG - chr8.1:3165179-3165198 None:intergenic 45.0%
!! CAAAAAAGTGGAGGCACTTG+TGG + chr8.1:3165073-3165092 MS.gene035630:CDS 45.0%
AGGCTCTGCATTAAGGTGCA+AGG + chr8.1:3165225-3165244 MS.gene035630:CDS 50.0%
GCACCCAGAGAGAAGTCGTT+GGG - chr8.1:3165505-3165524 None:intergenic 55.0%
CGGCCCAACGACTTCTCTCT+GGG + chr8.1:3165499-3165518 MS.gene035630:intron 60.0%
GGCACCCAGAGAGAAGTCGT+TGG - chr8.1:3165506-3165525 None:intergenic 60.0%
TCGGCCCAACGACTTCTCTC+TGG + chr8.1:3165498-3165517 MS.gene035630:intron 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 3164928 3166397 3164928 ID=MS.gene035630
chr8.1 mRNA 3164928 3166397 3164928 ID=MS.gene035630.t1;Parent=MS.gene035630
chr8.1 exon 3164928 3165349 3164928 ID=MS.gene035630.t1.exon1;Parent=MS.gene035630.t1
chr8.1 CDS 3164928 3165349 3164928 ID=cds.MS.gene035630.t1;Parent=MS.gene035630.t1
chr8.1 exon 3165701 3165881 3165701 ID=MS.gene035630.t1.exon2;Parent=MS.gene035630.t1
chr8.1 CDS 3165701 3165881 3165701 ID=cds.MS.gene035630.t1;Parent=MS.gene035630.t1
chr8.1 exon 3166043 3166198 3166043 ID=MS.gene035630.t1.exon3;Parent=MS.gene035630.t1
chr8.1 CDS 3166043 3166198 3166043 ID=cds.MS.gene035630.t1;Parent=MS.gene035630.t1
chr8.1 exon 3166296 3166397 3166296 ID=MS.gene035630.t1.exon4;Parent=MS.gene035630.t1
chr8.1 CDS 3166296 3166397 3166296 ID=cds.MS.gene035630.t1;Parent=MS.gene035630.t1
Gene Sequence

>MS.gene035630

ATGGTTGGTGCAATCTCATCAACAGATGTGCAAAGACATAACGCGGGTAACATGAATGATATGATCAGTGACTTACCTGAGGACGTTCTTCTTGATATTCATTCTTTAGTCCCTACCAAAGATGCCGTAAAAACATCCATATTGGCAAAAAAGTGGAGGCACTTGTGGACTCATCTACGCTTCTTTGATTTTCAAATTCCTCATTTTTGTGATGGGTTACATCACCAAAATTTGAAGTTAGCAGATTGCCTCTTCGATCTAGTTGGAAGACTATTGCACAAATCTGATCGGATAGAAAGGCTCTGCATTAAGGTGCAAGGAATTGATATGTATGATGCATGGAAAGTTAATCTTTTGTTATCTTATGCACTGAAGCACAAAGTCCTTGATCTTAAACTTTCCCTAGAATTTATAGATTCTAGGTTTGAGTTACCCCATAATTTCTCATCTTCTCACTCATTGAATAAGTTGTGTTTAGAATCTGGCGTTCATATGTTTATACCTGATGGTATTTGCTTCCCAAATCTGAAGACATTATATCTTTCACGTCTTTCCTTCAATAATGAGATGTCGGCCCAACGACTTCTCTCTGGGTGCCCTATCCTTGAAGAGTTGACCTTATATAAATGCATATGGGTTAACATAAATCAAATATGTGTTGCAATTTCCACATTAAGGAAGTTAACCCTCCATTTTAAATCGTCTTTTCTAGATTATGGCCATTTTCCAAGTTGTATAGTCATAATTGATGCTGTGAATCTTTTGTCTTTAAGTTGCACGAGCAATCCAGCTATAAAATATATTCTTGTTAAACCAACCTCCATAGTTGATGCATGTATTGATCTTAAGTTTTCCTATCCTGAAAATCCACTATATGTTTCTCATTGTGCAATGGAGCTATTGAGTGAACTTGGTAGTGTCAAGTCTCTCAAACTAACACATAATACTATTCAGGTATGTTTGAATATTTACTTATCATCTTCTTTTTTTTTTTTTTAAATATGTATAAGAAAATGACTATTCACAATGTTGTCTCAGTTATGTGCTTCTTTATTCGTTGCATGTTTTTTATATGTCACAATAATGAATCTATTTTTGTTTCCTTAATTAACCAGCGTTTTTCTTATCCAAGGAACTGTGGTCATCTTTTGCCTTCATTCAACAATTTGACGAACCTTGACATGATTTTAGGGTATTGTTATGTTAGCTGTGAACTATTAGTAGACATTCTATGTAAGACTCCTAAACTGGAAGTTCTTCACATTTCCAAGGTATATCTTACATCATTTCTTCTATTTTGTTATGTCCTGTCTCACAACTAATATTATTATTTCTTTCCATTGACTTGTATATTGATTCTTGTTTTAGGGAGATTGCATAGACTTGGACAATGAAGACGTACTTGACATCAGATTCTTTGCCTTGTTGTATCAAATCTTCTCTCAAATTATGTTCTTTTTCAGATTTTGA

Protein sequence

>MS.gene035630.t1

MVGAISSTDVQRHNAGNMNDMISDLPEDVLLDIHSLVPTKDAVKTSILAKKWRHLWTHLRFFDFQIPHFCDGLHHQNLKLADCLFDLVGRLLHKSDRIERLCIKVQGIDMYDAWKVNLLLSYALKHKVLDLKLSLEFIDSSCTSNPAIKYILVKPTSIVDACIDLKFSYPENPLYVSHCAMELLSELGSVKSLKLTHNTIQRFSYPRNCGHLLPSFNNLTNLDMILGYCYVSCELLVDILCKTPKLEVLHISKGDCIDLDNEDVLDIRFFALLYQIFSQIMFFFRF