AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069763

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069763.t1 MTR_4g080787 70.714 280 79 1 2 281 200 476 1.12e-131 385
MS.gene069763.t1 MTR_4g080807 58.457 337 136 3 15 349 78 412 1.44e-122 359
MS.gene069763.t1 MTR_4g080773 66.292 267 90 0 2 268 200 466 9.96e-118 350
MS.gene069763.t1 MTR_4g080773 66.292 267 90 0 2 268 200 466 1.91e-117 349
MS.gene069763.t1 MTR_3g048290 63.509 285 99 2 2 282 194 477 4.75e-109 327
MS.gene069763.t1 MTR_4g080590 47.253 273 139 4 2 273 188 456 2.26e-63 208
MS.gene069763.t1 MTR_5g039950 45.185 270 117 3 2 268 178 419 1.78e-59 197
MS.gene069763.t1 MTR_4g130200 41.418 268 126 1 1 268 155 391 2.83e-56 188
MS.gene069763.t1 MTR_7g023330 44.610 269 140 4 4 268 132 395 3.16e-55 186
MS.gene069763.t1 MTR_7g023310 47.426 272 134 5 2 269 191 457 1.10e-51 178
MS.gene069763.t1 MTR_7g039630 52.717 184 84 2 2 182 160 343 2.05e-51 174
MS.gene069763.t1 MTR_4g081030 38.577 267 110 2 2 268 175 387 7.32e-40 145
MS.gene069763.t1 MTR_4g080590 51.648 182 83 4 2 182 188 365 4.70e-39 142
MS.gene069763.t1 MTR_2g094750 46.739 184 90 5 2 178 278 460 2.81e-33 129
MS.gene069763.t1 MTR_5g039970 51.852 135 62 2 2 133 188 322 2.85e-27 110
MS.gene069763.t1 MTR_2g094450 39.459 185 94 5 8 189 30 199 4.24e-24 98.2
MS.gene069763.t1 MTR_8g062270 31.169 231 137 10 6 228 200 416 1.04e-11 66.2
MS.gene069763.t1 MTR_2g102420 48.571 70 36 0 109 178 18 87 2.46e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069763.t1 AT4G14096 25.352 284 191 8 1 270 175 451 1.67e-14 74.7
MS.gene069763.t1 AT4G14096 25.352 284 191 8 1 270 175 451 1.67e-14 74.7
MS.gene069763.t1 AT5G22730 29.200 250 158 8 6 241 197 441 4.54e-14 73.6
MS.gene069763.t1 AT5G22730 29.200 250 158 8 6 241 197 441 4.54e-14 73.6
MS.gene069763.t1 AT3G59180 26.861 309 173 10 5 270 150 448 2.26e-13 71.2
MS.gene069763.t1 AT5G22660 29.388 245 152 11 5 236 178 414 2.68e-13 70.9
MS.gene069763.t1 AT1G66290 26.829 246 161 8 6 241 193 429 8.82e-13 69.3
MS.gene069763.t1 AT3G59210 26.207 290 182 8 5 270 182 463 2.99e-12 67.8
MS.gene069763.t1 AT3G59210 26.207 290 182 8 5 270 182 463 2.99e-12 67.8
MS.gene069763.t1 AT3G59210 26.207 290 182 8 5 270 182 463 2.99e-12 67.8
MS.gene069763.t1 AT3G59210 26.207 290 182 8 5 270 182 463 2.99e-12 67.8
MS.gene069763.t1 AT3G59210 26.207 290 182 8 5 270 182 463 2.99e-12 67.8
MS.gene069763.t1 AT3G59160 23.843 281 168 9 5 269 210 460 3.48e-12 67.8
MS.gene069763.t1 AT3G59200 23.906 297 184 9 4 270 181 465 4.22e-12 67.4
MS.gene069763.t1 AT3G59170 27.586 290 161 13 5 271 206 469 5.94e-12 67.0
MS.gene069763.t1 AT4G14103 27.513 189 122 4 1 181 177 358 7.28e-12 66.2
MS.gene069763.t1 AT4G14103 27.513 189 122 4 1 181 177 358 7.28e-12 66.2
MS.gene069763.t1 AT3G59200 29.016 193 104 7 4 175 181 361 1.67e-11 65.1
MS.gene069763.t1 AT1G58310 27.575 301 174 10 5 270 198 489 2.49e-11 65.1
MS.gene069763.t1 AT4G14103 28.492 179 113 4 5 175 181 352 6.71e-11 63.5
MS.gene069763.t1 AT5G53840 26.515 264 176 10 5 264 182 431 9.06e-11 63.2

Find 60 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACCTTGTACAATTGTTAT+TGG 0.244477 4.3:-32692734 MS.gene069763:CDS
ACTACGGAGGTCAAGTTAAC+TGG 0.323880 4.3:+32692549 None:intergenic
TGAAGTGTTTGCTGGGATTC+CGG 0.334316 4.3:-32692197 MS.gene069763:CDS
GACCTTGTACAATTGTTATT+GGG 0.339260 4.3:-32692733 MS.gene069763:CDS
GTGGAAGTTCTTGAGATTCC+TGG 0.341747 4.3:-32692127 MS.gene069763:CDS
TCAAGATAAACTTAACGAAT+TGG 0.345412 4.3:+32691889 None:intergenic
GAGAGAAGATTTGAAACAAC+AGG 0.360677 4.3:+32691956 None:intergenic
AAGGTCAACTCTTGTAAAAC+AGG 0.363675 4.3:+32692750 None:intergenic
ATGAGAAGGTGGATGATATC+TGG 0.370444 4.3:-32691806 MS.gene069763:CDS
ATCTCAAGAACTTCCACTTT+AGG 0.379741 4.3:+32692133 None:intergenic
AGATAGCACAACTTGTCCTT+TGG 0.387314 4.3:+32690145 None:intergenic
CTTCCACTTTAGGAGCCTTT+CGG 0.394536 4.3:+32692143 None:intergenic
GATAGCACAACTTGTCCTTT+GGG 0.407572 4.3:+32690146 None:intergenic
GTCTTCATCCTTGAAGCGTC+AGG 0.415487 4.3:+32690171 None:intergenic
TGATTAGGTACTTAACTACT+TGG 0.425971 4.3:-32692009 MS.gene069763:intron
TTCAAAATCGATGGTCAACT+TGG 0.438130 4.3:+32692671 None:intergenic
GTCCATCAGTACTTCATTAG+TGG 0.440471 4.3:+32692171 None:intergenic
ACACATCTTGAAGTGTTTGC+TGG 0.440808 4.3:-32692205 MS.gene069763:CDS
AGATTCATCGTCGTAGTAGC+TGG 0.457076 4.3:+32690576 None:intergenic
GGCCTCCTACATCATCCAAT+TGG 0.473236 4.3:+32691781 None:intergenic
CACATCTTGAAGTGTTTGCT+GGG 0.475981 4.3:-32692204 MS.gene069763:CDS
GCAGCATATGATTCACTACT+TGG 0.483641 4.3:+32692495 None:intergenic
GGATGATATCTGGAACCAAT+TGG 0.496991 4.3:-32691796 MS.gene069763:CDS
TCTCTGGTGCTTCCTGCTGC+AGG 0.500502 4.3:-32690382 MS.gene069763:intron
TCTCCGAAAGGCTCCTAAAG+TGG 0.514005 4.3:-32692146 MS.gene069763:CDS
AAAATAAAACGTACCACTCC+AGG 0.522108 4.3:+32692109 None:intergenic
AGTACTTCATTAGTGGTCTC+CGG 0.531587 4.3:+32692178 None:intergenic
TGTCCATTCTTACCTGCAGC+AGG 0.534614 4.3:+32690370 None:intergenic
GTGGAACACATTGAGTGCCT+TGG 0.536180 4.3:-32690438 MS.gene069763:CDS
TGTTAAATGTGGGAAGGAGA+TGG 0.538548 4.3:+32692233 None:intergenic
ATAGCACAACTTGTCCTTTG+GGG 0.541746 4.3:+32690147 None:intergenic
ATTGGATGATGTAGGAGGCC+CGG 0.545520 4.3:-32691778 MS.gene069763:CDS
TAGGTACTTAACTACTTGGA+TGG 0.558260 4.3:-32692005 MS.gene069763:CDS
ATGTGTCAAATTGTTAAATG+TGG 0.562573 4.3:+32692222 None:intergenic
ATCAATAGATGCATCAACTA+CGG 0.563483 4.3:+32692533 None:intergenic
GACCACTAATGAAGTACTGA+TGG 0.566068 4.3:-32692173 MS.gene069763:CDS
CTACGGAGGTCAAGTTAACT+GGG 0.576215 4.3:+32692550 None:intergenic
CATATGATTCACTACTTGGA+TGG 0.581308 4.3:+32692499 None:intergenic
AGAAATAAAGATATGAATCA+CGG 0.582339 4.3:+32690089 None:intergenic
ATTAGCTGATAATGAGAAGG+TGG 0.584272 4.3:-32691817 MS.gene069763:CDS
GCAAAAGAGTTCAAAATCGA+TGG 0.584518 4.3:+32692662 None:intergenic
TTTATTAGCTGATAATGAGA+AGG 0.585950 4.3:-32691820 MS.gene069763:CDS
CTCCCAATAACAATTGTACA+AGG 0.587766 4.3:+32692731 None:intergenic
TGTGTCAAATTGTTAAATGT+GGG 0.588530 4.3:+32692223 None:intergenic
GATGGTCAACTTGGTTAATG+TGG 0.589550 4.3:+32692680 None:intergenic
TAGCACAACTTGTCCTTTGG+GGG 0.590993 4.3:+32690148 None:intergenic
AGTTCTTGAGATTCCTGGAG+TGG 0.597460 4.3:-32692122 MS.gene069763:intron
TGGAACCAATTGGATGATGT+AGG 0.597577 4.3:-32691786 MS.gene069763:CDS
ACTATATAATAACACTCTTG+AGG 0.601018 4.3:-32692421 MS.gene069763:intron
TTTGATCTCCCCTAAATATG+GGG 0.602591 4.3:+32691860 None:intergenic
TGTGTATTTGAGCTATTGAG+TGG 0.623146 4.3:-32692468 MS.gene069763:CDS
AGCACAACTTGTCCTTTGGG+GGG 0.632285 4.3:+32690149 None:intergenic
AACCAATTGGATGATGTAGG+AGG 0.635148 4.3:-32691783 MS.gene069763:CDS
TCAAATTGTTAAATGTGGGA+AGG 0.635959 4.3:+32692227 None:intergenic
ACTGATGGACATTCTCCGAA+AGG 0.652765 4.3:-32692158 MS.gene069763:CDS
CTAGTGTCTTTACTACACAA+AGG 0.654654 4.3:-32692263 MS.gene069763:intron
AGGTCAACTCTTGTAAAACA+GGG 0.670121 4.3:+32692751 None:intergenic
AGAGAAGATTTGAAACAACA+GGG 0.683271 4.3:+32691957 None:intergenic
CAACAGTGAACGTTAGACCA+AGG 0.692408 4.3:+32690421 None:intergenic
AATAGATGCATCAACTACGG+AGG 0.700718 4.3:+32692536 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATATGTAATTAAATGAT+TGG - chr4.3:32691378-32691397 MS.gene069763:intron 10.0%
!! TCATTTAATTACATATATTT+AGG + chr4.3:32691377-32691396 None:intergenic 10.0%
!! TTGATAAAAAACATAAATTA+AGG + chr4.3:32691794-32691813 None:intergenic 10.0%
!!! TATGCTTTTTAAATTTTTAT+GGG - chr4.3:32690931-32690950 MS.gene069763:intron 10.0%
!!! TTATGCTTTTTAAATTTTTA+TGG - chr4.3:32690930-32690949 MS.gene069763:intron 10.0%
!! AGAAATAAAGATATGAATCA+CGG + chr4.3:32692771-32692790 None:intergenic 20.0%
!! ATACTTACACTTTACATAAT+AGG - chr4.3:32692325-32692344 MS.gene069763:intron 20.0%
!! ATTAAAGGAAATAGCATAAT+AGG + chr4.3:32692591-32692610 None:intergenic 20.0%
!! TAACTGATAAATTCATGAAA+AGG - chr4.3:32691581-32691600 MS.gene069763:intron 20.0%
!! TAAGTATGGTTTAAATATGT+CGG + chr4.3:32691330-32691349 None:intergenic 20.0%
!!! ATATTTTTAGTATGTGAAGT+AGG - chr4.3:32691947-32691966 MS.gene069763:CDS 20.0%
!!! ATGTAATTAAATGATTGGTT+AGG - chr4.3:32691383-32691402 MS.gene069763:intron 20.0%
!!! TTTTTAAAACTAGAGTCTTT+CGG - chr4.3:32691860-32691879 MS.gene069763:CDS 20.0%
! AAATCTGAAGGAACTAATTT+AGG + chr4.3:32692004-32692023 None:intergenic 25.0%
! AAATGTACCTAGTCTATATT+GGG - chr4.3:32691700-32691719 MS.gene069763:intron 25.0%
! AAGTATGGTTTAAATATGTC+GGG + chr4.3:32691329-32691348 None:intergenic 25.0%
! ACTAACCAAATATATGAACT+CGG + chr4.3:32691652-32691671 None:intergenic 25.0%
! ACTATATAATAACACTCTTG+AGG - chr4.3:32690436-32690455 MS.gene069763:CDS 25.0%
! ATGTGTCAAATTGTTAAATG+TGG + chr4.3:32690638-32690657 None:intergenic 25.0%
! ATTCCATAATATACTTAGAG+TGG - chr4.3:32691189-32691208 MS.gene069763:intron 25.0%
! CATAAAAAGCAAAGACAATA+AGG + chr4.3:32692547-32692566 None:intergenic 25.0%
! GACATATTTAAACCATACTT+AGG - chr4.3:32691329-32691348 MS.gene069763:intron 25.0%
! GAGAAAATAATTAATGAGTC+AGG + chr4.3:32692517-32692536 None:intergenic 25.0%
! TAAATTAGTTCCTTCAGATT+TGG - chr4.3:32692003-32692022 MS.gene069763:CDS 25.0%
! TCAAGATAAACTTAACGAAT+TGG + chr4.3:32690971-32690990 None:intergenic 25.0%
! TCTCATCTCATATAGATTAA+AGG + chr4.3:32692606-32692625 None:intergenic 25.0%
! TGAAAAATTCCCCATATTTA+GGG - chr4.3:32690988-32691007 MS.gene069763:intron 25.0%
! TGTGTCAAATTGTTAAATGT+GGG + chr4.3:32690637-32690656 None:intergenic 25.0%
! TTGAAAAATTCCCCATATTT+AGG - chr4.3:32690987-32691006 MS.gene069763:intron 25.0%
! TTGTTTAGTGATTCTTGATT+AGG - chr4.3:32690833-32690852 MS.gene069763:intron 25.0%
!! ACCTTCAAATTAAAAGTATG+TGG - chr4.3:32691900-32691919 MS.gene069763:CDS 25.0%
!! TTTATTAGCTGATAATGAGA+AGG - chr4.3:32691037-32691056 MS.gene069763:intron 25.0%
!!! ACAAGTTCTAGATTCTTTTT+GGG + chr4.3:32691265-32691284 None:intergenic 25.0%
!!! ACTTTTCTTTTGATTTGACT+TGG - chr4.3:32692093-32692112 MS.gene069763:intron 25.0%
!!! ATTGTCTTTGCTTTTTATGA+AGG - chr4.3:32692548-32692567 MS.gene069763:CDS 25.0%
!!! CTTTTCTTTTGATTTGACTT+GGG - chr4.3:32692094-32692113 MS.gene069763:intron 25.0%
AAAAAATCGATGACACAATC+CGG + chr4.3:32691101-32691120 None:intergenic 30.0%
AATGGAAACAAAACTACGTT+AGG + chr4.3:32690573-32690592 None:intergenic 30.0%
ATAACAGGACAAGTTATTAG+TGG - chr4.3:32692381-32692400 MS.gene069763:intron 30.0%
ATCAATAGATGCATCAACTA+CGG + chr4.3:32690327-32690346 None:intergenic 30.0%
ATTCATGAAAAGGTAATGCA+AGG - chr4.3:32691591-32691610 MS.gene069763:intron 30.0%
CAAATGTACCTAGTCTATAT+TGG - chr4.3:32691699-32691718 MS.gene069763:intron 30.0%
CAACCACTCTAAGTATATTA+TGG + chr4.3:32691195-32691214 None:intergenic 30.0%
CATGAAGATGATAAGCAAAT+AGG + chr4.3:32690470-32690489 None:intergenic 30.0%
CATTCAATCATACCTAAGTA+TGG + chr4.3:32691344-32691363 None:intergenic 30.0%
CTAAAGTCAACATTCTCATT+AGG + chr4.3:32690506-32690525 None:intergenic 30.0%
GAAAAATTCCCCATATTTAG+GGG - chr4.3:32690989-32691008 MS.gene069763:intron 30.0%
TCAAATTGTTAAATGTGGGA+AGG + chr4.3:32690633-32690652 None:intergenic 30.0%
TGACCTTGTACAATTGTTAT+TGG - chr4.3:32690123-32690142 MS.gene069763:CDS 30.0%
TGATTAGGTACTTAACTACT+TGG - chr4.3:32690848-32690867 MS.gene069763:intron 30.0%
TGTAGTAAAGACACTAGTAA+TGG + chr4.3:32690591-32690610 None:intergenic 30.0%
! AAATGATTGGTTAGGTCTTT+AGG - chr4.3:32691391-32691410 MS.gene069763:intron 30.0%
! AGAGAAGATTTGAAACAACA+GGG + chr4.3:32690903-32690922 None:intergenic 30.0%
! CTATATGAGATGAGATTTTG+TGG - chr4.3:32692611-32692630 MS.gene069763:CDS 30.0%
! GACCTTGTACAATTGTTATT+GGG - chr4.3:32690124-32690143 MS.gene069763:CDS 30.0%
! TTTTGATCTCCCCTAAATAT+GGG + chr4.3:32691001-32691020 None:intergenic 30.0%
!! ATAATGGAGAGGAAACTTTT+TGG - chr4.3:32691432-32691451 MS.gene069763:intron 30.0%
!! ATTTTGATCTCCCCTAAATA+TGG + chr4.3:32691002-32691021 None:intergenic 30.0%
!! TTCTTTGGAATAAGTTTCAC+AGG - chr4.3:32692046-32692065 MS.gene069763:intron 30.0%
!!! CAAGTTCTAGATTCTTTTTG+GGG + chr4.3:32691264-32691283 None:intergenic 30.0%
!!! GACAAGTTCTAGATTCTTTT+TGG + chr4.3:32691266-32691285 None:intergenic 30.0%
!!! GCCACATACTTTTAATTTGA+AGG + chr4.3:32691904-32691923 None:intergenic 30.0%
AAAAATCGATGACACAATCC+GGG + chr4.3:32691100-32691119 None:intergenic 35.0%
AAAATAAAACGTACCACTCC+AGG + chr4.3:32690751-32690770 None:intergenic 35.0%
AAGGTCAACTCTTGTAAAAC+AGG + chr4.3:32690110-32690129 None:intergenic 35.0%
AGAGATAGACGCTTAAATGT+GGG - chr4.3:32691519-32691538 MS.gene069763:intron 35.0%
AGATGTGTGTGTGATTATAG+AGG - chr4.3:32692191-32692210 MS.gene069763:CDS 35.0%
AGGTCAACTCTTGTAAAACA+GGG + chr4.3:32690109-32690128 None:intergenic 35.0%
ATCTCAAGAACTTCCACTTT+AGG + chr4.3:32690727-32690746 None:intergenic 35.0%
ATGTGTGTGTGATTATAGAG+GGG - chr4.3:32692193-32692212 MS.gene069763:CDS 35.0%
CATATGATTCACTACTTGGA+TGG + chr4.3:32690361-32690380 None:intergenic 35.0%
CCAAAAATCGTGTTATGCTT+CGG + chr4.3:32691241-32691260 None:intergenic 35.0%
CTAGTGTCTTTACTACACAA+AGG - chr4.3:32690594-32690613 MS.gene069763:intron 35.0%
CTCCCAATAACAATTGTACA+AGG + chr4.3:32690129-32690148 None:intergenic 35.0%
GATGTGTGTGTGATTATAGA+GGG - chr4.3:32692192-32692211 MS.gene069763:CDS 35.0%
GCAAAAGAGTTCAAAATCGA+TGG + chr4.3:32690198-32690217 None:intergenic 35.0%
GCTATCCGAGTTCATATATT+TGG - chr4.3:32691644-32691663 MS.gene069763:intron 35.0%
TAGATGTTGGAACACTTAGT+CGG - chr4.3:32691493-32691512 MS.gene069763:intron 35.0%
TAGGTACTTAACTACTTGGA+TGG - chr4.3:32690852-32690871 MS.gene069763:intron 35.0%
TATGTCGGGATGTTATAGTA+AGG + chr4.3:32691315-32691334 None:intergenic 35.0%
TGTGTATTTGAGCTATTGAG+TGG - chr4.3:32690389-32690408 MS.gene069763:CDS 35.0%
TGTGTGAAGTATGGACATAA+TGG - chr4.3:32691416-32691435 MS.gene069763:intron 35.0%
TTCAAAATCGATGGTCAACT+TGG + chr4.3:32690189-32690208 None:intergenic 35.0%
TTTGATCTCCCCTAAATATG+GGG + chr4.3:32691000-32691019 None:intergenic 35.0%
! AAGGAGAAGAAAGTTGATTG+AGG + chr4.3:32692134-32692153 None:intergenic 35.0%
! ATTAGCTGATAATGAGAAGG+TGG - chr4.3:32691040-32691059 MS.gene069763:intron 35.0%
! CAGTTCTACAACTTTTCTCT+GGG - chr4.3:32690081-32690100 MS.gene069763:CDS 35.0%
! GAGAGAAGATTTGAAACAAC+AGG + chr4.3:32690904-32690923 None:intergenic 35.0%
! GATTTTGAACTCTTTTGCGT+TGG - chr4.3:32690200-32690219 MS.gene069763:intron 35.0%
! TCAGTTCTACAACTTTTCTC+TGG - chr4.3:32690080-32690099 MS.gene069763:CDS 35.0%
! TTAGTGGCTGTTTCATTGTT+AGG - chr4.3:32692397-32692416 MS.gene069763:intron 35.0%
!! CTTTAGGATTGTGTGAAGTA+TGG - chr4.3:32691407-32691426 MS.gene069763:intron 35.0%
!! TGCTTGTAATTGCTGTTTCA+AGG - chr4.3:32690780-32690799 MS.gene069763:intron 35.0%
!! TTTTCAGGCTTCAAATTCTC+TGG - chr4.3:32692459-32692478 MS.gene069763:CDS 35.0%
!!! AGGAAACTTTTTGGTTTCAG+CGG - chr4.3:32691441-32691460 MS.gene069763:intron 35.0%
!!! TAACGTTCACTGTTGTTTTC+AGG - chr4.3:32692444-32692463 MS.gene069763:CDS 35.0%
AACCAATTGGATGATGTAGG+AGG - chr4.3:32691074-32691093 MS.gene069763:intron 40.0%
AATAGATGCATCAACTACGG+AGG + chr4.3:32690324-32690343 None:intergenic 40.0%
AATCCAGCAACCAAATCTGA+AGG + chr4.3:32692016-32692035 None:intergenic 40.0%
AGATAGCACAACTTGTCCTT+TGG + chr4.3:32692715-32692734 None:intergenic 40.0%
AGTACTTCATTAGTGGTCTC+CGG + chr4.3:32690682-32690701 None:intergenic 40.0%
ATAGCACAACTTGTCCTTTG+GGG + chr4.3:32692713-32692732 None:intergenic 40.0%
ATAGTAAGGCAAAACCACCA+AGG + chr4.3:32691301-32691320 None:intergenic 40.0%
ATGAGAAGGTGGATGATATC+TGG - chr4.3:32691051-32691070 MS.gene069763:intron 40.0%
CTAGTTCCTATGTGGAAAGT+GGG - chr4.3:32691544-32691563 MS.gene069763:intron 40.0%
GAAACAGCAATTACAAGCAC+TGG + chr4.3:32690779-32690798 None:intergenic 40.0%
GAGAGATAGACGCTTAAATG+TGG - chr4.3:32691518-32691537 MS.gene069763:intron 40.0%
GATAGCACAACTTGTCCTTT+GGG + chr4.3:32692714-32692733 None:intergenic 40.0%
GATGGTCAACTTGGTTAATG+TGG + chr4.3:32690180-32690199 None:intergenic 40.0%
GCAGCATATGATTCACTACT+TGG + chr4.3:32690365-32690384 None:intergenic 40.0%
GTCCATCAGTACTTCATTAG+TGG + chr4.3:32690689-32690708 None:intergenic 40.0%
GTGTTCCAACATCTAAGCAT+TGG + chr4.3:32691488-32691507 None:intergenic 40.0%
TGCTTATGATCTCAAGCAAG+TGG + chr4.3:32691726-32691745 None:intergenic 40.0%
TGGAACCAATTGGATGATGT+AGG - chr4.3:32691071-32691090 MS.gene069763:intron 40.0%
TGTTAAATGTGGGAAGGAGA+TGG + chr4.3:32690627-32690646 None:intergenic 40.0%
TTCAGCCAATGCTTAGATGT+TGG - chr4.3:32691480-32691499 MS.gene069763:intron 40.0%
TTGAAAAACCTGACGCTTCA+AGG - chr4.3:32692678-32692697 MS.gene069763:CDS 40.0%
TTGTCTTTCCAACTACACCT+TGG - chr4.3:32691281-32691300 MS.gene069763:intron 40.0%
TTTCGTTGCTGGAGACATAT+GGG - chr4.3:32692158-32692177 MS.gene069763:CDS 40.0%
! ACACATCTTGAAGTGTTTGC+TGG - chr4.3:32690652-32690671 MS.gene069763:intron 40.0%
! CACATCTTGAAGTGTTTGCT+GGG - chr4.3:32690653-32690672 MS.gene069763:intron 40.0%
! CCGAAGCATAACACGATTTT+TGG - chr4.3:32691238-32691257 MS.gene069763:intron 40.0%
! GAAGTATGGACATAATGGAG+AGG - chr4.3:32691421-32691440 MS.gene069763:intron 40.0%
! GGATGATATCTGGAACCAAT+TGG - chr4.3:32691061-32691080 MS.gene069763:intron 40.0%
! GGATTAAGTTGCAGGTTCTT+TGG - chr4.3:32692031-32692050 MS.gene069763:intron 40.0%
! TTTTCGTTGCTGGAGACATA+TGG - chr4.3:32692157-32692176 MS.gene069763:CDS 40.0%
!! GACCACTAATGAAGTACTGA+TGG - chr4.3:32690684-32690703 MS.gene069763:intron 40.0%
AAACCTCCCACTTTCCACAT+AGG + chr4.3:32691553-32691572 None:intergenic 45.0%
ACTACGGAGGTCAAGTTAAC+TGG + chr4.3:32690311-32690330 None:intergenic 45.0%
AGATTCATCGTCGTAGTAGC+TGG + chr4.3:32692284-32692303 None:intergenic 45.0%
AGTTCTTGAGATTCCTGGAG+TGG - chr4.3:32690735-32690754 MS.gene069763:intron 45.0%
CAAAACCACCAAGGTGTAGT+TGG + chr4.3:32691292-32691311 None:intergenic 45.0%
CAACAGTGAACGTTAGACCA+AGG + chr4.3:32692439-32692458 None:intergenic 45.0%
CAAGTGGCCCAATATAGACT+AGG + chr4.3:32691710-32691729 None:intergenic 45.0%
CCACTTTCCACATAGGAACT+AGG + chr4.3:32691546-32691565 None:intergenic 45.0%
CCTAGTTCCTATGTGGAAAG+TGG - chr4.3:32691543-32691562 MS.gene069763:intron 45.0%
CTACGGAGGTCAAGTTAACT+GGG + chr4.3:32690310-32690329 None:intergenic 45.0%
GGTAATGCAAGGTGAACTTG+AGG - chr4.3:32691602-32691621 MS.gene069763:intron 45.0%
GTGGAAGTTCTTGAGATTCC+TGG - chr4.3:32690730-32690749 MS.gene069763:intron 45.0%
TAGCACAACTTGTCCTTTGG+GGG + chr4.3:32692712-32692731 None:intergenic 45.0%
TCTCCAGCAACGAAAAGCAA+AGG + chr4.3:32692153-32692172 None:intergenic 45.0%
TCTTTCCAACTACACCTTGG+TGG - chr4.3:32691284-32691303 MS.gene069763:intron 45.0%
TGGAGACATATGGGGGAATT+TGG - chr4.3:32692167-32692186 MS.gene069763:CDS 45.0%
TTCAGCGGTATGTTTAAGCG+TGG - chr4.3:32691456-32691475 MS.gene069763:intron 45.0%
TTCGTTGCTGGAGACATATG+GGG - chr4.3:32692159-32692178 MS.gene069763:CDS 45.0%
! ACTGATGGACATTCTCCGAA+AGG - chr4.3:32690699-32690718 MS.gene069763:intron 45.0%
! CTTCCACTTTAGGAGCCTTT+CGG + chr4.3:32690717-32690736 None:intergenic 45.0%
! GTGGCTGTTTCATTGTTAGG+TGG - chr4.3:32692400-32692419 MS.gene069763:intron 45.0%
! GTTCCTTCAGATTTGGTTGC+TGG - chr4.3:32692010-32692029 MS.gene069763:intron 45.0%
! TGAAGTGTTTGCTGGGATTC+CGG - chr4.3:32690660-32690679 MS.gene069763:intron 45.0%
! TGGTTGCTGGATTAAGTTGC+AGG - chr4.3:32692023-32692042 MS.gene069763:intron 45.0%
!! TCTCCTTTGCTTTTCGTTGC+TGG - chr4.3:32692147-32692166 MS.gene069763:CDS 45.0%
!!! TTTTTTTTTTAAATAATAAC+AGG - chr4.3:32692366-32692385 MS.gene069763:intron 5.0%
AGCACAACTTGTCCTTTGGG+GGG + chr4.3:32692711-32692730 None:intergenic 50.0%
ATTGGATGATGTAGGAGGCC+CGG - chr4.3:32691079-32691098 MS.gene069763:intron 50.0%
CTTCCTGCTGCAGGTAAGAA+TGG - chr4.3:32692484-32692503 MS.gene069763:CDS 50.0%
GGCCTCCTACATCATCCAAT+TGG + chr4.3:32691079-32691098 None:intergenic 50.0%
GTGGAACACATTGAGTGCCT+TGG - chr4.3:32692419-32692438 MS.gene069763:intron 50.0%
GTTCCTATGTGGAAAGTGGG+AGG - chr4.3:32691547-32691566 MS.gene069763:intron 50.0%
TCGTTGCTGGAGACATATGG+GGG - chr4.3:32692160-32692179 MS.gene069763:CDS 50.0%
TCTCCGAAAGGCTCCTAAAG+TGG - chr4.3:32690711-32690730 MS.gene069763:intron 50.0%
TGTCCATTCTTACCTGCAGC+AGG + chr4.3:32692490-32692509 None:intergenic 50.0%
TGTGGGTCCTAGTTCCTATG+TGG - chr4.3:32691536-32691555 MS.gene069763:intron 50.0%
! GTCTTCATCCTTGAAGCGTC+AGG + chr4.3:32692689-32692708 None:intergenic 50.0%
CAAGGATGAAGACCCCCCAA+AGG - chr4.3:32692696-32692715 MS.gene069763:CDS 55.0%
TCTCTGGTGCTTCCTGCTGC+AGG - chr4.3:32692475-32692494 MS.gene069763:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 32690077 32692802 32690077 ID=MS.gene069763
chr4.3 mRNA 32690077 32692802 32690077 ID=MS.gene069763.t1;Parent=MS.gene069763
chr4.3 exon 32692422 32692802 32692422 ID=MS.gene069763.t1.exon1;Parent=MS.gene069763.t1
chr4.3 CDS 32692422 32692802 32692422 ID=cds.MS.gene069763.t1;Parent=MS.gene069763.t1
chr4.3 exon 32692123 32692281 32692123 ID=MS.gene069763.t1.exon2;Parent=MS.gene069763.t1
chr4.3 CDS 32692123 32692281 32692123 ID=cds.MS.gene069763.t1;Parent=MS.gene069763.t1
chr4.3 exon 32691759 32692024 32691759 ID=MS.gene069763.t1.exon3;Parent=MS.gene069763.t1
chr4.3 CDS 32691759 32692024 32691759 ID=cds.MS.gene069763.t1;Parent=MS.gene069763.t1
chr4.3 exon 32690564 32690600 32690564 ID=MS.gene069763.t1.exon4;Parent=MS.gene069763.t1
chr4.3 CDS 32690564 32690600 32690564 ID=cds.MS.gene069763.t1;Parent=MS.gene069763.t1
chr4.3 exon 32690383 32690460 32690383 ID=MS.gene069763.t1.exon5;Parent=MS.gene069763.t1
chr4.3 CDS 32690383 32690460 32690383 ID=cds.MS.gene069763.t1;Parent=MS.gene069763.t1
chr4.3 exon 32690077 32690208 32690077 ID=MS.gene069763.t1.exon6;Parent=MS.gene069763.t1
chr4.3 CDS 32690077 32690208 32690077 ID=cds.MS.gene069763.t1;Parent=MS.gene069763.t1
Gene Sequence

>MS.gene069763

ATGTCAGTTCTACAACTTTTCTCTGGGTGCCCTGTTTTACAAGAGTTGACCTTGTACAATTGTTATTGGGAGAATATAGAGCAGATCACTGTTGCAATTTCCACATTAACCAAGTTGACCATCGATTTTGAACTCTTTTGCGTTGGTTATGATCATGATATGACACTCACGATTGATGCTGTGAATCTTTTATCGTTAAGTTGCAGATGCATTCAAACAATAGAATTTATCCCAGTTAACTTGACCTCCGTAGTTGATGCATCTATTGATCTTGAGTATGACCATCCAAGTAGTGAATCATATGCTGCTCACTGTGTATTTGAGCTATTGAGTGGACTTAGTAGTGTTAAGTCTCTCACACTATATAATAACACTCTTGAGGTATGTTTGCCTATTTGCTTATCATCTTCATGTTTTTTTATAATACCTAATGAGAATGTTGACTTTAGTTATTTGTTTCTTATAATCGTTGCATATTTTCTGTATTACAGTACCTAACGTAGTTTTGTTTCCATTACTAGTGTCTTTACTACACAAAGGATACTCTCCATCTCCTTCCCACATTTAACAATTTGACACATCTTGAAGTGTTTGCTGGGATTCCGGAGACCACTAATGAAGTACTGATGGACATTCTCCGAAAGGCTCCTAAAGTGGAAGTTCTTGAGATTCCTGGAGTGGTACGTTTTATTTTCTTCACCAGTGCTTGTAATTGCTGTTTCAAGGCTAACAATAATATTATTTCAGTCAATTGACTTGTTTAGTGATTCTTGATTAGGTACTTAACTACTTGGATGGTGAAGATTTGATATTGAACGCAGTGCCCTGTTGTTTCAAATCTTCTCTCAATCGATTATGCTTTTTAAATTTTTATGGGAATGAATATGAAATCCAATTCGTTAAGTTTATCTTGAAAAATTCCCCATATTTAGGGGAGATCAAAATACACTGTTCAAGACATTTATTAGCTGATAATGAGAAGGTGGATGATATCTGGAACCAATTGGATGATGTAGGAGGCCCGGATTGTGTCATCGATTTTTTGTAAATATTCTTTCTTTTCTTCTCTCTTTTTTTTTTGTTGAAGTGATTCAGTTTACTAATTTAACTTCATTCCATAATATACTTAGAGTGGTTGTTCTTACTTTTACACTTGACATACCGAAGCATAACACGATTTTTGGCCCCAAAAAGAATCTAGAACTTGTCTTTCCAACTACACCTTGGTGGTTTTGCCTTACTATAACATCCCGACATATTTAAACCATACTTAGGTATGATTGAATGCTCAGTGAAGCCTAAATATATGTAATTAAATGATTGGTTAGGTCTTTAGGATTGTGTGAAGTATGGACATAATGGAGAGGAAACTTTTTGGTTTCAGCGGTATGTTTAAGCGTGGCTTCAGCCAATGCTTAGATGTTGGAACACTTAGTCGGCAGAGAGATAGACGCTTAAATGTGGGTCCTAGTTCCTATGTGGAAAGTGGGAGGTTTGATTGAAATAACTGATAAATTCATGAAAAGGTAATGCAAGGTGAACTTGAGGAATTGTCTTAGTTGTTTTAGCTATCCGAGTTCATATATTTGGTTAGTATTAGTACTTCAAAATTATTTATTCTTCAAATGTACCTAGTCTATATTGGGCCACTTGCTTGAGATCATAAGCAAAACTTTAATGCTGACTTGATTGACATGAAAGCTAATTCAGAAATCCTTAATTTATGTTTTTTATCAACATAATTTCTTAAAGCTCGTTTTCAGTAAAATCTTACTTTTTTATTTTTTTAAAACTAGAGTCTTTCGGATTATTTTCTTTGTATTACCTTCAAATTAAAAGTATGTGGCTGATGTTCTTTATCATAATTATAATATTTTTAGTATGTGAAGTAGGTTTTTATTCTATGATATTTTCATGATATGAGCCTAAATTAGTTCCTTCAGATTTGGTTGCTGGATTAAGTTGCAGGTTCTTTGGAATAAGTTTCACAGGAATATGAACAGTGTTTAAATTTAAACTTTTCTTTTGATTTGACTTGGGAATGATTTGTTATACCTCAATCAACTTTCTTCTCCTTTGCTTTTCGTTGCTGGAGACATATGGGGGAATTTGGAAGATGTGTGTGTGATTATAGAGGGGTTTGAGATCTTCATTCTGAAAGTATGTTGAGCTGCTCACTGTTATTTGTTTTCACTGTTTCAGCTCCAGCTACTACGACGATGAATCTGATGAAGATGAAGTGAGTAAAATACTTACACTTTACATAATAGGATGAGTTTTTTTTTTTTTTTTTTTTTTTAAATAATAACAGGACAAGTTATTAGTGGCTGTTTCATTGTTAGGTGGAACACATTGAGTGCCTTGGTCTAACGTTCACTGTTGTTTTCAGGCTTCAAATTCTCTGGTGCTTCCTGCTGCAGGTAAGAATGGACATGCTCCTGACTCATTAATTATTTTCTCATTCTCTCCTTATTGTCTTTGCTTTTTATGAAGGATTTGAGTTATAGTGAACCTATTATGCTATTTCCTTTAATCTATATGAGATGAGATTTTGTGGATTCTAAAGCATGAGAAATTGAACTTTCTAATCTCAGATGCACTTTGAAAAACCTGACGCTTCAAGGATGAAGACCCCCCAAAGGACAAGTTGTGCTATCTGTCTTAGTAGTGTTGAGTGTAGACTTTTGTCTGCCGTGATTCATATCTTTATTTCTGTCAAGTTATAG
Protein sequence

>MS.gene069763.t1

MSVLQLFSGCPVLQELTLYNCYWENIEQITVAISTLTKLTIDFELFCVGYDHDMTLTIDAVNLLSLSCRCIQTIEFIPVNLTSVVDASIDLEYDHPSSESYAAHCVFELLSGLSSVKSLTLYNNTLECLYYTKDTLHLLPTFNNLTHLEVFAGIPETTNEVLMDILRKAPKVEVLEIPGVVLNYLDGEDLILNAVPCCFKSSLNRLCFLNFYGNEYEIQFVKFILKNSPYLGEIKIHCSRHLLADNEKVDDIWNQLDDVGGPDCVIDFFSSYYDDESDEDEVEHIECLGLTFTVVFRLQILWCFLLQMHFEKPDASRMKTPQRTSCAICLSSVECRLLSAVIHIFISVKL