AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene91524


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91524.t1 MTR_5g040570 100.000 89 0 0 1 89 1 89 8.62e-61 181
MS.gene91524.t1 MTR_7g010150 100.000 89 0 0 1 89 1 89 1.06e-60 181
MS.gene91524.t1 MTR_8g467460 100.000 89 0 0 1 89 1 89 1.70e-60 180
MS.gene91524.t1 MTR_7g011050 66.292 89 14 1 1 89 1 73 6.19e-34 112
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91524.t1 AT1G26880 93.258 89 6 0 1 89 1 89 9.60e-57 171
MS.gene91524.t1 AT1G69620 93.258 89 6 0 1 89 1 89 2.05e-56 170
MS.gene91524.t1 AT1G26880 93.258 89 6 0 1 89 1 89 4.54e-56 168
MS.gene91524.t1 AT3G28900 89.888 89 9 0 1 89 1 89 4.72e-54 164

Find 30 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAACCGCCGCACTGTTAAC+AGG 0.305052 7.1:+85279814 MS.gene91524:CDS
TGGATCCTCTTTCCAGTAAC+AGG 0.314412 7.1:-85278551 None:intergenic
GGACCTAAGTGTCCTGTTAC+TGG 0.331250 7.1:+85278539 MS.gene91524:CDS
CGCACTGTTAACAGGGCTTA+TGG 0.356563 7.1:+85279822 MS.gene91524:CDS
AACACGATGCTGGTTGGATT+TGG 0.389836 7.1:-85278317 None:intergenic
TTAACAGTGCGGCGGTTCCT+AGG 0.468395 7.1:-85279810 None:intergenic
CCTTTCCCTAACAGCTCCTC+CGG 0.470814 7.1:-85279856 None:intergenic
TTTATCCGGAGGAGCTGTTA+GGG 0.501294 7.1:+85279851 MS.gene91524:CDS
TTTCCAGTAACAGGACACTT+AGG 0.509665 7.1:-85278542 None:intergenic
ACGATGCTGGTTGGATTTGG+TGG 0.532546 7.1:-85278314 None:intergenic
CATCGTGTTGTTAAGACACC+TGG 0.536949 7.1:+85278331 MS.gene91524:CDS
AGTGTCCTGTTACTGGAAAG+AGG 0.543803 7.1:+85278546 MS.gene91524:CDS
CTTAACAACACGATGCTGGT+TGG 0.545517 7.1:-85278323 None:intergenic
GTTACTGGAAAGAGGATCCA+AGG 0.571456 7.1:+85278554 MS.gene91524:CDS
ACAACTAAGAAGAGAGCTAG+TGG 0.577057 7.1:+85278518 MS.gene91524:CDS
GTGTCTTAACAACACGATGC+TGG 0.578379 7.1:-85278327 None:intergenic
TGGCATAGCTGTGTCTCCTA+CGG 0.580308 7.1:-85278294 None:intergenic
CCGGAGGAGCTGTTAGGGAA+AGG 0.595594 7.1:+85279856 MS.gene91524:CDS
GAACCGCCGCACTGTTAACA+GGG 0.611783 7.1:+85279815 MS.gene91524:CDS
CTAGACCTCTTGTATTCAGT+AGG 0.617053 7.1:-85279783 None:intergenic
TTACTGGAAAGAGGATCCAA+GGG 0.629653 7.1:+85278555 MS.gene91524:CDS
TGAGACCTACTGAATACAAG+AGG 0.631121 7.1:+85279778 MS.gene91524:CDS
ACAAGAGGTCTAGATTGCCT+AGG 0.636078 7.1:+85279793 MS.gene91524:CDS
TATTCAGTAGGTCTCAAGTG+TGG 0.667813 7.1:-85279771 None:intergenic
AAGCCCTGTTAACAGTGCGG+CGG 0.691394 7.1:-85279818 None:intergenic
ACTGTTAACAGGGCTTATGG+AGG 0.693206 7.1:+85279825 MS.gene91524:CDS
TTCAGAGACTCACATACCGT+AGG 0.721913 7.1:+85278278 MS.gene91524:CDS
CATAAGCCCTGTTAACAGTG+CGG 0.730145 7.1:-85279821 None:intergenic
TACTGGAAAGAGGATCCAAG+GGG 0.738216 7.1:+85278556 MS.gene91524:CDS
GTATGTGAGTCTCTGAACCA+TGG 0.739024 7.1:-85278272 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATAAAATCAATTATTTG+TGG + chr7.1:85278842-85278861 MS.gene91524:intron 10.0%
!! ATTTGAAAATTGAAAATATT+AGG - chr7.1:85278926-85278945 None:intergenic 10.0%
!!! TTTTTTAGAAATTAATATGT+TGG + chr7.1:85278600-85278619 MS.gene91524:intron 10.0%
!! TAACAAATCTTCAATAAAAA+TGG - chr7.1:85279256-85279275 None:intergenic 15.0%
!! TTTGCAATTATTAATGTTAA+TGG + chr7.1:85278807-85278826 MS.gene91524:intron 15.0%
!!! TTTGATTAATTTGTGAAAAT+GGG + chr7.1:85278460-85278479 MS.gene91524:intron 15.0%
!!! TTTTGATTAATTTGTGAAAA+TGG + chr7.1:85278459-85278478 MS.gene91524:intron 15.0%
!! GAATGAAAATTAAAAGTAGT+AGG - chr7.1:85279630-85279649 None:intergenic 20.0%
!!! TTAATACTACAATTTTGTGA+GGG - chr7.1:85279416-85279435 None:intergenic 20.0%
!!! TTAATATGTTGGTTATACAT+AGG + chr7.1:85278611-85278630 MS.gene91524:intron 20.0%
!!! TTTAATACTACAATTTTGTG+AGG - chr7.1:85279417-85279436 None:intergenic 20.0%
! AAACTATCTCTTCTGTAATA+GGG - chr7.1:85279114-85279133 None:intergenic 25.0%
! AATGTTCTTACTTCAATGTT+GGG + chr7.1:85278876-85278895 MS.gene91524:intron 25.0%
! AGATACCCTTTCTAAATTAT+TGG + chr7.1:85279436-85279455 MS.gene91524:intron 25.0%
! AGTCACCAATAATTTAGAAA+GGG - chr7.1:85279444-85279463 None:intergenic 25.0%
! ATCTATCATGTTTCAAACAT+AGG - chr7.1:85279280-85279299 None:intergenic 25.0%
! GAAAATTAAAAGTAGTAGGT+AGG - chr7.1:85279626-85279645 None:intergenic 25.0%
! GACTTGTATTATTCATCAAT+TGG + chr7.1:85278727-85278746 MS.gene91524:intron 25.0%
! GATATATGTGAGTTTATGAT+AGG + chr7.1:85278705-85278724 MS.gene91524:intron 25.0%
! GCATCATTGAAATTATACAA+TGG - chr7.1:85279529-85279548 None:intergenic 25.0%
! TGTAAAATAGGTGGAATATT+AGG + chr7.1:85278772-85278791 MS.gene91524:intron 25.0%
! TTATCATATTTGTTCTGTTC+TGG + chr7.1:85279732-85279751 MS.gene91524:intron 25.0%
! TTGAATTCATCTATGTTAGT+TGG - chr7.1:85278956-85278975 None:intergenic 25.0%
! TTGTCTGTGTTATGTAAAAT+AGG + chr7.1:85278760-85278779 MS.gene91524:intron 25.0%
AAGTGTCAAGATTATATGCA+GGG - chr7.1:85279602-85279621 None:intergenic 30.0%
CAAACTATCTCTTCTGTAAT+AGG - chr7.1:85279115-85279134 None:intergenic 30.0%
CAACACAATATTCAATCAGA+TGG - chr7.1:85279084-85279103 None:intergenic 30.0%
CAGTCACCAATAATTTAGAA+AGG - chr7.1:85279445-85279464 None:intergenic 30.0%
GAATGTTCTTACTTCAATGT+TGG + chr7.1:85278875-85278894 MS.gene91524:intron 30.0%
TCTGTGTTATGTAAAATAGG+TGG + chr7.1:85278763-85278782 MS.gene91524:intron 30.0%
! AAAATTGATGATAGCGTCAA+AGG - chr7.1:85279326-85279345 None:intergenic 30.0%
! AAAGTGTCAAGATTATATGC+AGG - chr7.1:85279603-85279622 None:intergenic 30.0%
!!! AAATGGGTTTGTGTTGTTTT+AGG + chr7.1:85278476-85278495 MS.gene91524:intron 30.0%
CAATTCTACAACATTGTGGA+AGG + chr7.1:85279548-85279567 MS.gene91524:intron 35.0%
GATGCAATTCTACAACATTG+TGG + chr7.1:85279544-85279563 MS.gene91524:intron 35.0%
TCATATAGATTCATACCCCT+TGG - chr7.1:85278574-85278593 None:intergenic 35.0%
TGACAAAGAAGAACATTACC+AGG - chr7.1:85278352-85278371 None:intergenic 35.0%
TTTGTTTGTACATGATGCTG+TGG + chr7.1:85278669-85278688 MS.gene91524:intron 35.0%
! CAATTTTGTGAGGGACAATA+AGG - chr7.1:85279407-85279426 None:intergenic 35.0%
!!! TGTTGTTTTAGGTGGAAAGT+TGG + chr7.1:85278487-85278506 MS.gene91524:intron 35.0%
AAACTTAGACGCTGTGTGAT+AGG - chr7.1:85279503-85279522 None:intergenic 40.0%
ACAACTAAGAAGAGAGCTAG+TGG + chr7.1:85278518-85278537 MS.gene91524:CDS 40.0%
ATAGCGTCAAAGGTCATAGT+TGG - chr7.1:85279316-85279335 None:intergenic 40.0%
CTAGACCTCTTGTATTCAGT+AGG - chr7.1:85279786-85279805 None:intergenic 40.0%
CTTCACAAACACAGCTGAAT+TGG - chr7.1:85279197-85279216 None:intergenic 40.0%
TATTCAGTAGGTCTCAAGTG+TGG - chr7.1:85279774-85279793 None:intergenic 40.0%
TGAGACCTACTGAATACAAG+AGG + chr7.1:85279778-85279797 MS.gene91524:CDS 40.0%
TTACTGGAAAGAGGATCCAA+GGG + chr7.1:85278555-85278574 MS.gene91524:CDS 40.0%
TTTCCAGTAACAGGACACTT+AGG - chr7.1:85278545-85278564 None:intergenic 40.0%
TTTGTGAGGGACAATAAGGA+CGG - chr7.1:85279403-85279422 None:intergenic 40.0%
!!! GCTTATGGAGGTGTTTTATC+CGG + chr7.1:85279837-85279856 MS.gene91524:CDS 40.0%
!!! TCATTGCTTTTTCTCCCTGA+TGG + chr7.1:85279657-85279676 MS.gene91524:intron 40.0%
!!! TGGGTTTGTGTTGTTTTAGG+TGG + chr7.1:85278479-85278498 MS.gene91524:intron 40.0%
ACAAGAGGTCTAGATTGCCT+AGG + chr7.1:85279793-85279812 MS.gene91524:CDS 45.0%
ACTGTTAACAGGGCTTATGG+AGG + chr7.1:85279825-85279844 MS.gene91524:CDS 45.0%
AGTGCATGTCATGACCATCA+GGG - chr7.1:85279674-85279693 None:intergenic 45.0%
AGTGTCCTGTTACTGGAAAG+AGG + chr7.1:85278546-85278565 MS.gene91524:CDS 45.0%
CATAAGCCCTGTTAACAGTG+CGG - chr7.1:85279824-85279843 None:intergenic 45.0%
CTTAACAACACGATGCTGGT+TGG - chr7.1:85278326-85278345 None:intergenic 45.0%
GTGTCTTAACAACACGATGC+TGG - chr7.1:85278330-85278349 None:intergenic 45.0%
GTTACTGGAAAGAGGATCCA+AGG + chr7.1:85278554-85278573 MS.gene91524:CDS 45.0%
TACTGGAAAGAGGATCCAAG+GGG + chr7.1:85278556-85278575 MS.gene91524:CDS 45.0%
TGGATCCTCTTTCCAGTAAC+AGG - chr7.1:85278554-85278573 None:intergenic 45.0%
TTCAGAGACTCACATACCGT+AGG + chr7.1:85278278-85278297 MS.gene91524:CDS 45.0%
TTTATCCGGAGGAGCTGTTA+GGG + chr7.1:85279851-85279870 MS.gene91524:CDS 45.0%
! AACACGATGCTGGTTGGATT+TGG - chr7.1:85278320-85278339 None:intergenic 45.0%
! CATCGTGTTGTTAAGACACC+TGG + chr7.1:85278331-85278350 MS.gene91524:CDS 45.0%
! TTTTATCCGGAGGAGCTGTT+AGG + chr7.1:85279850-85279869 MS.gene91524:CDS 45.0%
!!! TATGGAGGTGTTTTATCCGG+AGG + chr7.1:85279840-85279859 MS.gene91524:CDS 45.0%
CGCACTGTTAACAGGGCTTA+TGG + chr7.1:85279822-85279841 MS.gene91524:CDS 50.0%
GAGTGCATGTCATGACCATC+AGG - chr7.1:85279675-85279694 None:intergenic 50.0%
GGACCTAAGTGTCCTGTTAC+TGG + chr7.1:85278539-85278558 MS.gene91524:CDS 50.0%
GTCTCAAGTGTGGAATCTGC+AGG - chr7.1:85279764-85279783 None:intergenic 50.0%
TGGCATAGCTGTGTCTCCTA+CGG - chr7.1:85278297-85278316 None:intergenic 50.0%
! ACGATGCTGGTTGGATTTGG+TGG - chr7.1:85278317-85278336 None:intergenic 50.0%
AAGCCCTGTTAACAGTGCGG+CGG - chr7.1:85279821-85279840 None:intergenic 55.0%
GAACCGCCGCACTGTTAACA+GGG + chr7.1:85279815-85279834 MS.gene91524:CDS 55.0%
TTAACAGTGCGGCGGTTCCT+AGG - chr7.1:85279813-85279832 None:intergenic 55.0%
GGAACCGCCGCACTGTTAAC+AGG + chr7.1:85279814-85279833 MS.gene91524:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 85278274 85279877 85278274 ID=MS.gene91524
chr7.1 mRNA 85278274 85279877 85278274 ID=MS.gene91524.t1;Parent=MS.gene91524
chr7.1 exon 85278274 85278352 85278274 ID=MS.gene91524.t1.exon1;Parent=MS.gene91524.t1
chr7.1 CDS 85278274 85278352 85278274 ID=cds.MS.gene91524.t1;Parent=MS.gene91524.t1
chr7.1 exon 85278498 85278577 85278498 ID=MS.gene91524.t1.exon2;Parent=MS.gene91524.t1
chr7.1 CDS 85278498 85278577 85278498 ID=cds.MS.gene91524.t1;Parent=MS.gene91524.t1
chr7.1 exon 85279768 85279877 85279768 ID=MS.gene91524.t1.exon3;Parent=MS.gene91524.t1
chr7.1 CDS 85279768 85279877 85279768 ID=cds.MS.gene91524.t1;Parent=MS.gene91524.t1
Gene Sequence

>MS.gene91524

ATGGTTCAGAGACTCACATACCGTAGGAGACACAGCTATGCCACCAAATCCAACCAGCATCGTGTTGTTAAGACACCTGGTAATGTTCTTCTTTGTCAAATTGTTCTGTTTTTTCTTGTTCTAATCTATGTTATGATGTTTTTGATTTTTTCTTAAAAATTGCATTTTTTTGTTGTTGAAGTGTTTTTTGATTAATTTGTGAAAATGGGTTTGTGTTGTTTTAGGTGGAAAGTTGGTTTATCAGACAACTAAGAAGAGAGCTAGTGGACCTAAGTGTCCTGTTACTGGAAAGAGGATCCAAGGGGTATGAATCTATATGATATCATTTTTTTAGAAATTAATATGTTGGTTATACATAGGATCTGTGATTCATTGTGTTGATTCTATTTTTTTTGTTTGTTTGTACATGATGCTGTGGAAAAATGTAGATTGATATATGTGAGTTTATGATAGGACTTGTATTATTCATCAATTGGTTGTGTGAATTTGTCTGTGTTATGTAAAATAGGTGGAATATTAGGATTGTTTTGTGTTTTGCAATTATTAATGTTAATGGAATGCTGAGCTTATTATAAAATCAATTATTTGTGGTTTATTGAGCGAATGTTCTTACTTCAATGTTGGGTTTACTGTTTGATTGTTTGAAAATCCTAATATTTTCAATTTTCAAATCTATATTCCAACTAACATAGATGAATTCAATCAAATCATTTGTCTTGTATTTTGATTAATGTTAATCGCTGAGCATATTATGAAATCAATTACTGTGTTTTTTTTAGTTAGCTTTTACTGTCTTTTATGCATGCTCCATCTGATTGAATATTGTGTTGTTTACGTCCCTATTACAGAAGAGATAGTTTGTTTTCTGCTTTAATTATGTGATGATTCAGTTTTTTTGAAGACGTAAATAACTTAATTCACCAATTCAGCTGTGTTTGTGAAGTGAATTAGTATAGTCTGTCTGTTTTTTTTGTTAAGTCCATTTTTATTGAAGATTTGTTAGCCTATGTTTGAAACATGATAGATATAATAATCATTTCCAACTATGACCTTTGACGCTATCATCAATTTTATAATGACAAAAATTAGATAGTTAGACTTAGATGCAATTCTTTTGAGATTGACACCGTCCTTATTGTCCCTCACAAAATTGTAGTATTAAAGATACCCTTTCTAAATTATTGGTGACTGTTTTTCTTATTTAGTTACAAACAACTAGAGCAGTGCCTATCACACAGCGTCTAAGTTTTGTCCATTGTATAATTTCAATGATGCAATTCTACAACATTGTGGAAGGAACATACAATGAATGTATTACTGTGTTACCCTGCATATAATCTTGACACTTTCCTACCTACTACTTTTAATTTTCATTCAAAGATTTCATTGCTTTTTCTCCCTGATGGTCATGACATGCACTCTTTATCTTTAGTTGCAAGTTGATCAATGTGTGTTCTCTTATCATATTTGTTCTGTTCTGGACTTGACCTGCAGATTCCACACTTGAGACCTACTGAATACAAGAGGTCTAGATTGCCTAGGAACCGCCGCACTGTTAACAGGGCTTATGGAGGTGTTTTATCCGGAGGAGCTGTTAGGGAAAG

Protein sequence

>MS.gene91524.t1

MVQRLTYRRRHSYATKSNQHRVVKTPGGKLVYQTTKKRASGPKCPVTGKRIQGIPHLRPTEYKRSRLPRNRRTVNRAYGGVLSGGAVRE