AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene93341


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93341.t1 MTR_3g037750 100.000 82 0 0 1 82 22 103 2.84e-56 169
MS.gene93341.t1 MTR_7g010580 90.000 70 7 0 13 82 40 109 1.72e-43 137
MS.gene93341.t1 MTR_6g012360 88.406 69 8 0 14 82 39 107 1.43e-41 132
MS.gene93341.t1 MTR_7g090590 72.000 75 21 0 8 82 46 120 3.15e-34 114
MS.gene93341.t1 MTR_3g007900 66.667 66 22 0 17 82 89 154 2.82e-28 100
MS.gene93341.t1 MTR_5g095990 66.667 66 22 0 17 82 84 149 3.77e-28 99.4
MS.gene93341.t1 MTR_5g036860 61.765 68 26 0 15 82 25 92 5.35e-25 89.7
MS.gene93341.t1 MTR_5g036720 58.824 68 28 0 15 82 25 92 2.42e-22 83.2
MS.gene93341.t1 MTR_5g036880 57.353 68 29 0 15 82 25 92 4.00e-22 82.4
MS.gene93341.t1 MTR_5g036640 58.333 60 25 0 23 82 24 83 3.42e-20 77.4
MS.gene93341.t1 MTR_8g085470 64.706 68 24 0 15 82 28 95 2.14e-18 73.2
MS.gene93341.t1 MTR_3g102660 49.153 59 30 0 24 82 27 85 2.70e-13 59.7
MS.gene93341.t1 MTR_1g106915 44.444 63 35 0 20 82 28 90 4.28e-13 59.7
MS.gene93341.t1 MTR_1g096370 41.463 82 46 2 1 82 16 95 1.74e-12 58.2
MS.gene93341.t1 MTR_1g025250 41.772 79 45 1 5 82 22 100 4.35e-12 57.4
MS.gene93341.t1 MTR_1g025220 42.169 83 44 2 1 82 22 101 1.58e-11 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93341.t1 AT1G74670 76.829 82 18 1 1 82 21 101 6.22e-41 130
MS.gene93341.t1 AT5G15230 66.667 84 26 1 1 82 23 106 1.24e-35 117
MS.gene93341.t1 AT5G15230 69.737 76 23 0 7 82 8 83 9.76e-35 114
MS.gene93341.t1 AT3G10185 66.667 78 23 1 5 82 48 122 3.29e-23 86.3
MS.gene93341.t1 AT2G30810 64.286 70 25 0 13 82 37 106 1.31e-20 79.0
MS.gene93341.t1 AT3G02885 67.647 68 22 0 15 82 30 97 2.42e-20 78.2
MS.gene93341.t1 AT1G10588 45.205 73 39 1 11 82 18 90 1.79e-14 63.2
MS.gene93341.t1 AT2G39540 38.889 72 44 0 11 82 16 87 8.32e-14 61.2
MS.gene93341.t1 AT1G10588 48.333 60 30 1 24 82 30 89 4.05e-13 59.7
MS.gene93341.t1 AT1G22690 47.541 61 32 0 22 82 59 119 2.73e-11 55.8
MS.gene93341.t1 AT1G22690 47.541 61 32 0 22 82 59 119 2.73e-11 55.8
MS.gene93341.t1 AT1G22690 47.541 61 32 0 22 82 59 119 2.73e-11 55.8
MS.gene93341.t1 AT4G09600 36.585 82 46 1 1 82 24 99 6.68e-11 54.3
MS.gene93341.t1 AT5G59845 39.726 73 43 1 11 82 17 89 7.85e-11 53.9

Find 21 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTATTTACAGGGGAATTA+TGG 0.224887 3.2:-27000773 MS.gene93341:intron
TTTCAATTTCAAGGGCATTT+TGG 0.264806 3.2:+26999093 None:intergenic
GGTCTTCCAATTGTTGTAAC+AGG 0.291635 3.2:+26999128 None:intergenic
CATGACTTGTTTCAATTTCA+AGG 0.329598 3.2:+26999084 None:intergenic
TGTGTTCCTCCTGGCTATTA+TGG 0.372312 3.2:-26999168 MS.gene93341:CDS
TGTGTCCCTGTTACAACAAT+TGG 0.425313 3.2:-26999134 MS.gene93341:CDS
GCAAAAGATGCTGCTCAGTA+TGG 0.425821 3.2:-27000919 MS.gene93341:CDS
CATCTTCTTGTGCATTGAGA+TGG 0.446525 3.2:+26999255 None:intergenic
ATCTTCTTGTGCATTGAGAT+GGG 0.464024 3.2:+26999256 None:intergenic
AAATGTCTCTGTGTTCCTCC+TGG 0.478257 3.2:-26999177 MS.gene93341:CDS
AGGAGGAACACAGAGACATT+TGG 0.480481 3.2:+26999179 None:intergenic
ATGACTTGTTTCAATTTCAA+GGG 0.510609 3.2:+26999085 None:intergenic
GATTTGTTACCATAATAGCC+AGG 0.572427 3.2:+26999159 None:intergenic
CGGTAGCTTTGAAGACTCCC+AGG 0.578508 3.2:+27000749 None:intergenic
GTCTTCCAATTGTTGTAACA+GGG 0.578624 3.2:+26999129 None:intergenic
TACAGGGGAATTATGGACCT+GGG 0.587624 3.2:-27000766 MS.gene93341:intron
TCTCAATGCACAAGAAGATG+TGG 0.608334 3.2:-26999252 MS.gene93341:CDS
TTACAGGGGAATTATGGACC+TGG 0.609160 3.2:-27000767 MS.gene93341:intron
AACAATTGGAAGACCAAGCG+TGG 0.618322 3.2:-26999120 MS.gene93341:CDS
AATTGGAAGACCAAGCGTGG+AGG 0.716723 3.2:-26999117 MS.gene93341:CDS
TTGTTACCATAATAGCCAGG+AGG 0.723243 3.2:+26999162 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTATAATTAAATACAG+AGG - chr3.2:26999619-26999638 MS.gene93341:intron 10.0%
!! TAAAAACTTAGTAAAAAAAA+GGG - chr3.2:26999966-26999985 MS.gene93341:intron 10.0%
!!! TTAATTTTGTACATAAAATA+TGG - chr3.2:26999316-26999335 MS.gene93341:intron 10.0%
!! AAAAAAAGTCTATGTTAAAA+TGG + chr3.2:26999726-26999745 None:intergenic 15.0%
!! AAAAACTTAGTAAAAAAAAG+GGG - chr3.2:26999967-26999986 MS.gene93341:intron 15.0%
!! GTAAAAACTTAGTAAAAAAA+AGG - chr3.2:26999965-26999984 MS.gene93341:intron 15.0%
!! TTAAATAAAGAATATTTCGT+GGG - chr3.2:27000636-27000655 MS.gene93341:intron 15.0%
!! TTTAAATAAAGAATATTTCG+TGG - chr3.2:27000635-27000654 MS.gene93341:intron 15.0%
!!! AAAAAGTTGGTATATTTATA+GGG - chr3.2:27000431-27000450 MS.gene93341:intron 15.0%
!!! AATTTTTTCGATTTATTTAC+AGG - chr3.2:26999218-26999237 MS.gene93341:CDS 15.0%
!!! AGTATCATTAGTTAATTTTT+AGG + chr3.2:26999854-26999873 None:intergenic 15.0%
!!! ATATATCCATTTTAAATTAC+TGG + chr3.2:27000485-27000504 None:intergenic 15.0%
!!! ATGAATTTTTATATTTTTGG+TGG + chr3.2:27000215-27000234 None:intergenic 15.0%
!!! ATTTTTTCGATTTATTTACA+GGG - chr3.2:26999219-26999238 MS.gene93341:CDS 15.0%
!!! TAGTGATTGAAAATTTTATA+GGG + chr3.2:27000070-27000089 None:intergenic 15.0%
!!! TTTTATGTACAAAATTAAGT+TGG + chr3.2:26999314-26999333 None:intergenic 15.0%
!!! TTTTTTACTAAGTTTTTACT+AGG + chr3.2:26999964-26999983 None:intergenic 15.0%
!! AAATCACCAGTAATTTAAAA+TGG - chr3.2:27000476-27000495 MS.gene93341:intron 20.0%
!! CACATAAACAAATATATAAG+TGG + chr3.2:26999895-26999914 None:intergenic 20.0%
!! CACTTATATATTTGTTTATG+TGG - chr3.2:26999893-26999912 MS.gene93341:intron 20.0%
!! TTCAGAAACATATAATAAGT+GGG + chr3.2:26999521-26999540 None:intergenic 20.0%
!! TTTCAGAAACATATAATAAG+TGG + chr3.2:26999522-26999541 None:intergenic 20.0%
!!! ATATGAGTTTACTTTAAAAG+AGG - chr3.2:27000339-27000358 MS.gene93341:intron 20.0%
!!! ATCATTAGTTAATTTTTAGG+CGG + chr3.2:26999851-26999870 None:intergenic 20.0%
!!! CAAAAAGTTGGTATATTTAT+AGG - chr3.2:27000430-27000449 MS.gene93341:intron 20.0%
!!! CATGCTTTTTTTTTCTTTTA+TGG - chr3.2:26999409-26999428 MS.gene93341:intron 20.0%
!!! GAAAAGTTGGTATATTTATT+GGG + chr3.2:27000010-27000029 None:intergenic 20.0%
!!! GTAGTGATTGAAAATTTTAT+AGG + chr3.2:27000071-27000090 None:intergenic 20.0%
!!! TAAATTACTGGTGATTTTTA+GGG + chr3.2:27000473-27000492 None:intergenic 20.0%
!!! TATGAGTTTACTTTAAAAGA+GGG - chr3.2:27000340-27000359 MS.gene93341:intron 20.0%
!!! TATGTGGCAATTTTATTTTT+TGG - chr3.2:26999909-26999928 MS.gene93341:intron 20.0%
!!! TTAAATTACTGGTGATTTTT+AGG + chr3.2:27000474-27000493 None:intergenic 20.0%
!!! TTTTAGGGTTAATATGTTTT+TGG + chr3.2:27000458-27000477 None:intergenic 20.0%
!!! TTTTTTCGATTTATTTACAG+GGG - chr3.2:26999220-26999239 MS.gene93341:CDS 20.0%
! AAATGAATGAATGTTAGAGA+CGG + chr3.2:26999661-26999680 None:intergenic 25.0%
! ATTTATTTACAGGGGAATTA+TGG - chr3.2:26999228-26999247 MS.gene93341:CDS 25.0%
! CATGAGGAAATTTCTTTAAA+TGG + chr3.2:26999590-26999609 None:intergenic 25.0%
! TAAAGAAATTTCCTCATGTA+TGG - chr3.2:26999592-26999611 MS.gene93341:intron 25.0%
! TCAGAAACATATAATAAGTG+GGG + chr3.2:26999520-26999539 None:intergenic 25.0%
!! AAGTTGAAGGAAAATTTTAG+AGG + chr3.2:27000418-27000399 None:intergenic 25.0%
!! AGTTGAAGGAAAATTTTAGA+GGG + chr3.2:27000419-27000400 None:intergenic 25.0%
!! TAATTTTTGTACCATACATG+AGG + chr3.2:26999606-26999625 None:intergenic 25.0%
!! TTTTGCAAAATGCCTATTAA+AGG + chr3.2:26999162-26999181 None:intergenic 25.0%
!!! ATGACTTGTTTCAATTTCAA+GGG + chr3.2:27000919-27000938 None:intergenic 25.0%
!!! ATTAAGTTGGTCTACAAAAA+TGG + chr3.2:26999301-26999320 None:intergenic 25.0%
!!! CGAAAAGTTGGTATATTTAT+TGG + chr3.2:27000011-27000030 None:intergenic 25.0%
!!! TGAAGATGGTTAGTTTTAAT+GGG - chr3.2:27000371-27000390 MS.gene93341:intron 25.0%
AAATATTAAGCAATGCTCTG+AGG - chr3.2:26999774-26999793 MS.gene93341:intron 30.0%
AAATGCCTATTAAAGGAACT+AGG + chr3.2:26999155-26999174 None:intergenic 30.0%
AATATTAAGCAATGCTCTGA+GGG - chr3.2:26999775-26999794 MS.gene93341:intron 30.0%
AATTCTCAAAACAGAGAGAA+AGG + chr3.2:26999120-26999139 None:intergenic 30.0%
GAGAGACTAAAACAAAAAGT+TGG - chr3.2:27000418-27000437 MS.gene93341:intron 30.0%
GAGCATTCAAACAGAAATAT+AGG + chr3.2:27000516-27000535 None:intergenic 30.0%
TCTTTCCTAGTTCCTTTAAT+AGG - chr3.2:26999147-26999166 MS.gene93341:CDS 30.0%
TTAATGGGACTAAAAGTTGA+AGG - chr3.2:27000386-27000405 MS.gene93341:intron 30.0%
TTATAGGGACTAAAAGTTGA+AGG + chr3.2:27000055-27000074 None:intergenic 30.0%
! AAACTAACCATCTTCACTTT+TGG + chr3.2:27000367-27000386 None:intergenic 30.0%
! ATGGATATATGATTGACTCA+CGG + chr3.2:26999275-26999294 None:intergenic 30.0%
!! CATGACTTGTTTCAATTTCA+AGG + chr3.2:27000920-27000939 None:intergenic 30.0%
!!! GTGAAGATGGTTAGTTTTAA+TGG - chr3.2:27000370-27000389 MS.gene93341:intron 30.0%
!!! GTGGCAATTTTATTTTTTGG+TGG - chr3.2:26999912-26999931 MS.gene93341:intron 30.0%
!!! TTTCAATTTCAAGGGCATTT+TGG + chr3.2:27000911-27000930 None:intergenic 30.0%
ACTAGGTACTTTGACGAATA+TGG + chr3.2:26999947-26999966 None:intergenic 35.0%
ATCTTCTTGTGCATTGAGAT+GGG + chr3.2:27000748-27000767 None:intergenic 35.0%
CTAGGTACTTTGACGAATAT+GGG + chr3.2:26999946-26999965 None:intergenic 35.0%
GTCTTCCAATTGTTGTAACA+GGG + chr3.2:27000875-27000894 None:intergenic 35.0%
GTTAACACAATGCAGTCTAA+TGG - chr3.2:27000689-27000708 MS.gene93341:intron 35.0%
TAAAGGAACTAGGAAAGAAG+TGG + chr3.2:26999145-26999164 None:intergenic 35.0%
TCTGAGAGAAATTGAATGAG+TGG - chr3.2:27000563-27000582 MS.gene93341:intron 35.0%
TGAATGAGTGGAATCAAACA+TGG - chr3.2:27000575-27000594 MS.gene93341:intron 35.0%
TGGTCTACAAAAATGGAGTA+TGG + chr3.2:26999294-26999313 None:intergenic 35.0%
! TTTTGACAGAAGAACATGCA+TGG + chr3.2:27000794-27000813 None:intergenic 35.0%
!! GATTTGTTACCATAATAGCC+AGG + chr3.2:27000845-27000864 None:intergenic 35.0%
CATCTTCTTGTGCATTGAGA+TGG + chr3.2:27000749-27000768 None:intergenic 40.0%
GGTCTTCCAATTGTTGTAAC+AGG + chr3.2:27000876-27000895 None:intergenic 40.0%
TCTCAATGCACAAGAAGATG+TGG - chr3.2:27000749-27000768 MS.gene93341:intron 40.0%
TGTGTCCCTGTTACAACAAT+TGG - chr3.2:27000867-27000886 MS.gene93341:intron 40.0%
TTGTTACCATAATAGCCAGG+AGG + chr3.2:27000842-27000861 None:intergenic 40.0%
! AGGCGGCTAATTTTCATTGA+TGG + chr3.2:26999834-26999853 None:intergenic 40.0%
AAATGTCTCTGTGTTCCTCC+TGG - chr3.2:27000824-27000843 MS.gene93341:intron 45.0%
AACAATTGGAAGACCAAGCG+TGG - chr3.2:27000881-27000900 MS.gene93341:intron 45.0%
AGAGGGACCAAAAGTGAAGA+TGG - chr3.2:27000357-27000376 MS.gene93341:intron 45.0%
AGGAGGAACACAGAGACATT+TGG + chr3.2:27000825-27000844 None:intergenic 45.0%
GAGGGACGAAAACGAAAAGT+TGG + chr3.2:27000023-27000042 None:intergenic 45.0%
GCAAAAGATGCTGCTCAGTA+TGG - chr3.2:26999082-26999101 MS.gene93341:CDS 45.0%
TACAGGGGAATTATGGACCT+GGG - chr3.2:26999235-26999254 MS.gene93341:CDS 45.0%
TGTGTTCCTCCTGGCTATTA+TGG - chr3.2:27000833-27000852 MS.gene93341:intron 45.0%
TTACAGGGGAATTATGGACC+TGG - chr3.2:26999234-26999253 MS.gene93341:CDS 45.0%
!!! AAAAAATTCTAATTTTTTTT+GGG + chr3.2:27000273-27000254 None:intergenic 5.0%
!!! AAAAAATTCTAATTTTTTTT+TGG - chr3.2:27000254-27000273 MS.gene93341:intron 5.0%
!!! AAAAATTCTAATTTTTTTTT+GGG - chr3.2:27000255-27000274 MS.gene93341:intron 5.0%
!!! AATATGAATTTTTATATTTT+TGG + chr3.2:27000218-27000237 None:intergenic 5.0%
!!! TAAAAAATTCTAATTTTTTT+TGG + chr3.2:27000272-27000253 None:intergenic 5.0%
AATTGGAAGACCAAGCGTGG+AGG - chr3.2:27000884-27000903 MS.gene93341:intron 50.0%
!! CGGTAGCTTTGAAGACTCCC+AGG + chr3.2:26999255-26999274 None:intergenic 55.0%
!! GCATTTTGGTCCTCCACGCT+TGG + chr3.2:27000897-27000916 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 26999076 27000947 26999076 ID=MS.gene93341
chr3.2 mRNA 26999076 27000947 26999076 ID=MS.gene93341.t1;Parent=MS.gene93341
chr3.2 exon 27000915 27000947 27000915 ID=MS.gene93341.t1.exon1;Parent=MS.gene93341.t1
chr3.2 CDS 27000915 27000947 27000915 ID=cds.MS.gene93341.t1;Parent=MS.gene93341.t1
chr3.2 exon 27000750 27000783 27000750 ID=MS.gene93341.t1.exon2;Parent=MS.gene93341.t1
chr3.2 CDS 27000750 27000783 27000750 ID=cds.MS.gene93341.t1;Parent=MS.gene93341.t1
chr3.2 exon 26999076 26999282 26999076 ID=MS.gene93341.t1.exon3;Parent=MS.gene93341.t1
chr3.2 CDS 26999076 26999282 26999076 ID=cds.MS.gene93341.t1;Parent=MS.gene93341.t1
Gene Sequence

>MS.gene93341

GTTATGGCAAAAGATGCTGCTCAGTATGGCAGTGTGAGTATCCTTTCTCTCTGTTTTGAGAATTTTCCACTTCTTTCCTAGTTCCTTTAATAGGCATTTTGCAAAAGTTTCATAAATTTTACTTAAAATTAGTTTGTTTACTAATTTTTTCGATTTATTTACAGGGGAATTATGGACCTGGGAGTCTTCAAAGCTACCGTGAGTCAATCATATATCCATACTCCATTTTTGTAGACCAACTTAATTTTGTACATAAAATATGGCTTTTCAAGTTGCATCATGCACTCTTAAAGTTTTAACTCATTAATTTTCTTTAATTATGAGACAGTTAAACATGCTTTTTTTTTCTTTTATGGATTACAGTTAAACATACTTTTAATATTTGTAAAATTTCGTTGATTTAAATTTATTTTGTACTTAATTTTTCTTATAAGTTTCACACCCCACTTATTATATGTTTCTGAAATCTCTCGTAAAATAGTCATTTTAGTACTTAAATATATGAATGATGCCATTTAAAGAAATTTCCTCATGTATGGTACAAAAATTATAATTAAATACAGAGGAATGTTATGAATAACACCGTCTCTAACATTCATTCATTTATTAGATGAAACACATATGAATTTCATTTCTTTTTATATTGTCCATTTTAACATAGACTTTTTTTATCTTGTACTCTTTTTAAATATGTGAGAAAATATTAAGCAATGCTCTGAGGGCACTGTTTAACATTCTCGAAAAAAAAAAAATATCCATCAATGAAAATTAGCCGCCTAAAAATTAACTAATGATACTTTTAAACTAATTTTGACTCCACTTATATATTTGTTTATGTGGCAATTTTATTTTTTGGTGGATAAATTACCCATATTCGTCAAAGTACCTAGTAAAAACTTAGTAAAAAAAAGGGGTTAAATATGTTTTTGATCCCAATAAATATACCAACTTTTCGTTTTCGTCCCTCTAAAATTTTCCTTCAACTTTTAGTCCCTATAAAATTTTCAATCACTACTTTTAAGTTAATTTTTGTATTTTTTAATTAAATTGTGCAGAAATGTGTAGATATTGTAAAAAGAAATCCCAAAAAAAATTAGAATTTTTTAACAAAACATAAATTAATTATGAATTTTTAACCACCAAAAATATAAAAATTCATATTAAATTCATGTTTTGTTAAAAAATTCTAATTTTTTTTTGGGAGAGATTTTTATAGTATTATGAATTTTTGTGCAAAATTTTATTCAATAATATGAATTTTACATATGAGTTTACTTTAAAAGAGGGACCAAAAGTGAAGATGGTTAGTTTTAATGGGACTAAAAGTTGAAGGAAAATTTTAGAGAGACTAAAACAAAAAGTTGGTATATTTATAGGGACCAAAAACATATTAACCCTAAAAATCACCAGTAATTTAAAATGGATATATATGTTTGACCTATATTTCTGTTTGAATGCTCAACAAAAAAATAAGTTAGAATAATCAATCTGAGAGAAATTGAATGAGTGGAATCAAACATGGCACAAAACTTAGTTGAGTTGAGACTAGATCAACTAAATTTAAATAAAGAATATTTCGTGGGAAAAAAAAACACACTTTAACTGATTATCAAGTTAACACAATGCAGTCTAATGGATTTACGTTTGTGAAATTGATTTGATCAGAGTGCCCATCTCAATGCACAAGAAGATGTGGACAGACACAATATCACAAGCCATGCATGTTCTTCTGTCAAAAATGTTGTGCCAAATGTCTCTGTGTTCCTCCTGGCTATTATGGTAACAAATCTGTGTGTCCCTGTTACAACAATTGGAAGACCAAGCGTGGAGGACCAAAATGCCCTTGAAATTGAAACAAGTCATGATCATTAA

Protein sequence

>MS.gene93341.t1

VMAKDAAQYGSGNYGPGSLQSYQCPSQCTRRCGQTQYHKPCMFFCQKCCAKCLCVPPGYYGNKSVCPCYNNWKTKRGGPKCPUNUNKSUSL