Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94064.t1 | RHN40480.1 | 90.6 | 415 | 24 | 1 | 1 | 415 | 1 | 400 | 7.90E-197 | 696.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94064.t1 | A0A396GHL1 | 90.6 | 415 | 24 | 1 | 1 | 415 | 1 | 400 | 5.7e-197 | 696.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049351 | MS.gene94064 | 0.88797 | 8.93E-73 | -1.69E-46 |
| MS.gene049356 | MS.gene94064 | 0.802142 | 6.46E-49 | -1.69E-46 |
| MS.gene049369 | MS.gene94064 | 0.809692 | 1.67E-50 | -1.69E-46 |
| MS.gene049758 | MS.gene94064 | 0.808121 | 3.62E-50 | -1.69E-46 |
| MS.gene049794 | MS.gene94064 | 0.815675 | 8.19E-52 | -1.69E-46 |
| MS.gene050301 | MS.gene94064 | 0.844433 | 7.58E-59 | -1.69E-46 |
| MS.gene050397 | MS.gene94064 | 0.816895 | 4.37E-52 | -1.69E-46 |
| MS.gene050520 | MS.gene94064 | 0.804319 | 2.29E-49 | -1.69E-46 |
| MS.gene050538 | MS.gene94064 | 0.814589 | 1.43E-51 | -1.69E-46 |
| MS.gene050593 | MS.gene94064 | 0.812455 | 4.20E-51 | -1.69E-46 |
| MS.gene050670 | MS.gene94064 | 0.864851 | 9.03E-65 | -1.69E-46 |
| MS.gene050812 | MS.gene94064 | 0.812395 | 4.33E-51 | -1.69E-46 |
| MS.gene050971 | MS.gene94064 | 0.818946 | 1.50E-52 | -1.69E-46 |
| MS.gene05105 | MS.gene94064 | 0.840144 | 1.04E-57 | -1.69E-46 |
| MS.gene051255 | MS.gene94064 | 0.861251 | 1.17E-63 | -1.69E-46 |
| MS.gene051360 | MS.gene94064 | 0.837351 | 5.47E-57 | -1.69E-46 |
| MS.gene051387 | MS.gene94064 | 0.861694 | 8.57E-64 | -1.69E-46 |
| MS.gene051525 | MS.gene94064 | 0.837054 | 6.52E-57 | -1.69E-46 |
| MS.gene051582 | MS.gene94064 | 0.811204 | 7.87E-51 | -1.69E-46 |
| MS.gene051694 | MS.gene94064 | 0.81078 | 9.72E-51 | -1.69E-46 |
| MS.gene051860 | MS.gene94064 | 0.810355 | 1.20E-50 | -1.69E-46 |
| MS.gene051877 | MS.gene94064 | 0.833443 | 5.32E-56 | -1.69E-46 |
| MS.gene052011 | MS.gene94064 | -0.842264 | 2.87E-58 | -1.69E-46 |
| MS.gene052188 | MS.gene94064 | 0.8489 | 4.56E-60 | -1.69E-46 |
| MS.gene052253 | MS.gene94064 | 0.850486 | 1.65E-60 | -1.69E-46 |
| MS.gene052310 | MS.gene94064 | 0.810185 | 1.31E-50 | -1.69E-46 |
| MS.gene052322 | MS.gene94064 | 0.833753 | 4.45E-56 | -1.69E-46 |
| MS.gene052414 | MS.gene94064 | 0.80456 | 2.04E-49 | -1.69E-46 |
| MS.gene052421 | MS.gene94064 | 0.811709 | 6.11E-51 | -1.69E-46 |
| MS.gene052481 | MS.gene94064 | 0.802016 | 6.85E-49 | -1.69E-46 |
| MS.gene052484 | MS.gene94064 | 0.803007 | 4.28E-49 | -1.69E-46 |
| MS.gene052485 | MS.gene94064 | 0.847913 | 8.56E-60 | -1.69E-46 |
| MS.gene052982 | MS.gene94064 | 0.820481 | 6.71E-53 | -1.69E-46 |
| MS.gene053107 | MS.gene94064 | 0.830177 | 3.40E-55 | -1.69E-46 |
| MS.gene053169 | MS.gene94064 | 0.834384 | 3.09E-56 | -1.69E-46 |
| MS.gene053209 | MS.gene94064 | 0.823517 | 1.33E-53 | -1.69E-46 |
| MS.gene05335 | MS.gene94064 | 0.817522 | 3.16E-52 | -1.69E-46 |
| MS.gene053758 | MS.gene94064 | 0.809173 | 2.16E-50 | -1.69E-46 |
| MS.gene053836 | MS.gene94064 | 0.805727 | 1.16E-49 | -1.69E-46 |
| MS.gene054010 | MS.gene94064 | 0.826409 | 2.76E-54 | -1.69E-46 |
| MS.gene054011 | MS.gene94064 | 0.828228 | 1.01E-54 | -1.69E-46 |
| MS.gene054012 | MS.gene94064 | 0.833725 | 4.52E-56 | -1.69E-46 |
| MS.gene054013 | MS.gene94064 | 0.826567 | 2.53E-54 | -1.69E-46 |
| MS.gene054123 | MS.gene94064 | 0.831262 | 1.84E-55 | -1.69E-46 |
| MS.gene054183 | MS.gene94064 | 0.805239 | 1.47E-49 | -1.69E-46 |
| MS.gene054556 | MS.gene94064 | 0.846597 | 1.97E-59 | -1.69E-46 |
| MS.gene05505 | MS.gene94064 | 0.822524 | 2.26E-53 | -1.69E-46 |
| MS.gene055116 | MS.gene94064 | 0.808008 | 3.82E-50 | -1.69E-46 |
| MS.gene055277 | MS.gene94064 | 0.851369 | 9.28E-61 | -1.69E-46 |
| MS.gene055369 | MS.gene94064 | 0.815778 | 7.76E-52 | -1.69E-46 |
| MS.gene055390 | MS.gene94064 | 0.836833 | 7.42E-57 | -1.69E-46 |
| MS.gene055412 | MS.gene94064 | 0.808474 | 3.04E-50 | -1.69E-46 |
| MS.gene055422 | MS.gene94064 | 0.808859 | 2.52E-50 | -1.69E-46 |
| MS.gene055564 | MS.gene94064 | 0.809619 | 1.73E-50 | -1.69E-46 |
| MS.gene055604 | MS.gene94064 | 0.832605 | 8.59E-56 | -1.69E-46 |
| MS.gene055909 | MS.gene94064 | 0.823664 | 1.23E-53 | -1.69E-46 |
| MS.gene055911 | MS.gene94064 | 0.830371 | 3.05E-55 | -1.69E-46 |
| MS.gene05602 | MS.gene94064 | 0.832005 | 1.21E-55 | -1.69E-46 |
| MS.gene056407 | MS.gene94064 | 0.860947 | 1.45E-63 | -1.69E-46 |
| MS.gene056410 | MS.gene94064 | 0.851936 | 6.41E-61 | -1.69E-46 |
| MS.gene056511 | MS.gene94064 | 0.815898 | 7.30E-52 | -1.69E-46 |
| MS.gene056659 | MS.gene94064 | 0.824407 | 8.21E-54 | -1.69E-46 |
| MS.gene056723 | MS.gene94064 | 0.804082 | 2.56E-49 | -1.69E-46 |
| MS.gene056796 | MS.gene94064 | 0.862156 | 6.19E-64 | -1.69E-46 |
| MS.gene056902 | MS.gene94064 | 0.861106 | 1.30E-63 | -1.69E-46 |
| MS.gene057011 | MS.gene94064 | 0.82283 | 1.92E-53 | -1.69E-46 |
| MS.gene057124 | MS.gene94064 | 0.82369 | 1.21E-53 | -1.69E-46 |
| MS.gene057146 | MS.gene94064 | 0.806472 | 8.09E-50 | -1.69E-46 |
| MS.gene057448 | MS.gene94064 | 0.802369 | 5.80E-49 | -1.69E-46 |
| MS.gene057450 | MS.gene94064 | 0.80232 | 5.93E-49 | -1.69E-46 |
| MS.gene057749 | MS.gene94064 | 0.803013 | 4.27E-49 | -1.69E-46 |
| MS.gene057823 | MS.gene94064 | 0.815915 | 7.24E-52 | -1.69E-46 |
| MS.gene058002 | MS.gene94064 | 0.809365 | 1.96E-50 | -1.69E-46 |
| MS.gene058006 | MS.gene94064 | 0.803109 | 4.08E-49 | -1.69E-46 |
| MS.gene058007 | MS.gene94064 | 0.819131 | 1.36E-52 | -1.69E-46 |
| MS.gene058082 | MS.gene94064 | 0.827167 | 1.82E-54 | -1.69E-46 |
| MS.gene05811 | MS.gene94064 | 0.862646 | 4.37E-64 | -1.69E-46 |
| MS.gene058120 | MS.gene94064 | 0.806497 | 7.99E-50 | -1.69E-46 |
| MS.gene058233 | MS.gene94064 | 0.803923 | 2.76E-49 | -1.69E-46 |
| MS.gene058293 | MS.gene94064 | 0.812827 | 3.49E-51 | -1.69E-46 |
| MS.gene058323 | MS.gene94064 | 0.827538 | 1.48E-54 | -1.69E-46 |
| MS.gene058365 | MS.gene94064 | 0.804136 | 2.50E-49 | -1.69E-46 |
| MS.gene058376 | MS.gene94064 | 0.825149 | 5.49E-54 | -1.69E-46 |
| MS.gene05842 | MS.gene94064 | 0.809094 | 2.24E-50 | -1.69E-46 |
| MS.gene058660 | MS.gene94064 | 0.849336 | 3.45E-60 | -1.69E-46 |
| MS.gene058700 | MS.gene94064 | 0.848812 | 4.83E-60 | -1.69E-46 |
| MS.gene058948 | MS.gene94064 | 0.845404 | 4.15E-59 | -1.69E-46 |
| MS.gene058993 | MS.gene94064 | 0.805252 | 1.46E-49 | -1.69E-46 |
| MS.gene05907 | MS.gene94064 | 0.858531 | 7.73E-63 | -1.69E-46 |
| MS.gene059294 | MS.gene94064 | 0.807755 | 4.33E-50 | -1.69E-46 |
| MS.gene059420 | MS.gene94064 | 0.857202 | 1.92E-62 | -1.69E-46 |
| MS.gene059536 | MS.gene94064 | 0.820894 | 5.39E-53 | -1.69E-46 |
| MS.gene05959 | MS.gene94064 | 0.812404 | 4.31E-51 | -1.69E-46 |
| MS.gene059676 | MS.gene94064 | 0.837325 | 5.56E-57 | -1.69E-46 |
| MS.gene059688 | MS.gene94064 | 0.853525 | 2.26E-61 | -1.69E-46 |
| MS.gene059692 | MS.gene94064 | 0.825585 | 4.33E-54 | -1.69E-46 |
| MS.gene059695 | MS.gene94064 | 0.830147 | 3.46E-55 | -1.69E-46 |
| MS.gene059711 | MS.gene94064 | 0.81001 | 1.43E-50 | -1.69E-46 |
| MS.gene05984 | MS.gene94064 | 0.84024 | 9.80E-58 | -1.69E-46 |
| MS.gene060403 | MS.gene94064 | 0.813724 | 2.21E-51 | -1.69E-46 |
| MS.gene060804 | MS.gene94064 | 0.820795 | 5.68E-53 | -1.69E-46 |
| MS.gene060837 | MS.gene94064 | 0.866796 | 2.19E-65 | -1.69E-46 |
| MS.gene06090 | MS.gene94064 | 0.83933 | 1.69E-57 | -1.69E-46 |
| MS.gene06092 | MS.gene94064 | 0.816744 | 4.72E-52 | -1.69E-46 |
| MS.gene060986 | MS.gene94064 | 0.826181 | 3.12E-54 | -1.69E-46 |
| MS.gene061154 | MS.gene94064 | 0.876132 | 1.78E-68 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94064.t1 | MTR_8g044030 | 95.012 | 421 | 15 | 1 | 1 | 415 | 1 | 421 | 0.0 | 813 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94064.t1 | AT5G59500 | 57.933 | 416 | 154 | 6 | 1 | 415 | 1 | 396 | 1.21e-165 | 471 |
| MS.gene94064.t1 | AT5G59500 | 71.875 | 288 | 81 | 0 | 128 | 415 | 8 | 295 | 1.17e-162 | 459 |
Find 87 sgRNAs with CRISPR-Local
Find 98 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTATAATGCTTGCAGTTTA+TGG | 0.139273 | 8.4:-56371706 | MS.gene94064:CDS |
| GGCAAGTTCACATCCCATTT+TGG | 0.141939 | 8.4:+56371457 | None:intergenic |
| CCAACTTCTGTTGTGCAATT+TGG | 0.154490 | 8.4:-56371241 | MS.gene94064:CDS |
| CTCGCCGTCATGACCCAAAA+TGG | 0.232595 | 8.4:-56371471 | MS.gene94064:CDS |
| CTTAACTTTGCTTGCATATT+CGG | 0.243735 | 8.4:+56371036 | None:intergenic |
| TTAGCTGGACTTGGATTTAC+TGG | 0.258205 | 8.4:-56371741 | MS.gene94064:CDS |
| TTCATTGGAAATTGAGCATT+TGG | 0.258403 | 8.4:+56371996 | None:intergenic |
| TACTTAAACAAACAACAAAA+AGG | 0.259298 | 8.4:-56372038 | MS.gene94064:CDS |
| CATTGGCTTGCAGCATTTGA+AGG | 0.262632 | 8.4:-56371391 | MS.gene94064:CDS |
| TGCTCTTAAATCCTTTCTTC+TGG | 0.262743 | 8.4:-56371771 | MS.gene94064:CDS |
| TTTATGGGTTATACTGCTTT+TGG | 0.267587 | 8.4:-56371690 | MS.gene94064:CDS |
| GCTTTAATGGTCGAAACTTT+TGG | 0.284591 | 8.4:-56371070 | MS.gene94064:CDS |
| AGAATCTATGCTGGCTTCAT+TGG | 0.292712 | 8.4:+56371981 | None:intergenic |
| ATTGACAACTAATAAATAAA+AGG | 0.297190 | 8.4:+56371001 | None:intergenic |
| CTATAATGCTTGCAGTTTAT+GGG | 0.298189 | 8.4:-56371705 | MS.gene94064:CDS |
| GATGGAGCAATCTTGGAATC+TGG | 0.331382 | 8.4:-56371342 | MS.gene94064:CDS |
| TTGCATATTCGGTGTATATC+TGG | 0.332659 | 8.4:+56371047 | None:intergenic |
| AGCTATGGAGGAGGCTTTAA+TGG | 0.336552 | 8.4:-56371083 | MS.gene94064:CDS |
| GGCAAACATATTTGAGCAAT+TGG | 0.343810 | 8.4:-56371650 | MS.gene94064:CDS |
| GGATGTATGTTAGCTGGACT+TGG | 0.353195 | 8.4:-56371750 | MS.gene94064:CDS |
| CCAGGTGGATGGAGCAATCT+TGG | 0.372620 | 8.4:-56371349 | MS.gene94064:CDS |
| CTACCTTGAAGGCTGGCCTT+TGG | 0.382262 | 8.4:-56371569 | MS.gene94064:CDS |
| TCTTCTGGATGTATGTTAGC+TGG | 0.387873 | 8.4:-56371756 | MS.gene94064:CDS |
| AGTGTCCGGAACCGGTTATA+TGG | 0.389277 | 8.4:-56371505 | MS.gene94064:CDS |
| TTCAGTGCAGGAATCATGAT+TGG | 0.409000 | 8.4:-56371415 | MS.gene94064:CDS |
| AAACCAAAGGCCAGCCTTCA+AGG | 0.411112 | 8.4:+56371566 | None:intergenic |
| AACTAACACGCTTAGGTCTT+GGG | 0.412113 | 8.4:+56372100 | None:intergenic |
| AATCTCCATATAACCGGTTC+CGG | 0.414018 | 8.4:+56371500 | None:intergenic |
| AAACTAACACGCTTAGGTCT+TGG | 0.420283 | 8.4:+56372099 | None:intergenic |
| GTTCCACCAGCTTATTTCAA+TGG | 0.424534 | 8.4:-56371592 | MS.gene94064:CDS |
| CATAATAATCTCCATATAAC+CGG | 0.427250 | 8.4:+56371494 | None:intergenic |
| TCAACGTTAGTGTCCGGAAC+CGG | 0.428166 | 8.4:-56371513 | MS.gene94064:CDS |
| TATTTCAATGGCTACCTTGA+AGG | 0.429721 | 8.4:-56371580 | MS.gene94064:CDS |
| TTGGAAACACATGCTTGGTC+AGG | 0.432668 | 8.4:-56371671 | MS.gene94064:CDS |
| GTCAACTTCACAACATCAAT+TGG | 0.433542 | 8.4:+56371841 | None:intergenic |
| AATTGAAAACTAACACGCTT+AGG | 0.440837 | 8.4:+56372093 | None:intergenic |
| GAAATTGAGCATTTGGGAAG+AGG | 0.452427 | 8.4:+56372003 | None:intergenic |
| GATTTACTGGCCATGGTTGG+TGG | 0.460830 | 8.4:-56371728 | MS.gene94064:CDS |
| TGAAATAAGCTGGTGGAACA+AGG | 0.470249 | 8.4:+56371596 | None:intergenic |
| CCAAATTGCACAACAGAAGT+TGG | 0.474890 | 8.4:+56371241 | None:intergenic |
| TTTCAATTGAATCACAGCTA+TGG | 0.478964 | 8.4:-56372078 | MS.gene94064:CDS |
| TGATTCCTGCACTGAACAAA+AGG | 0.485115 | 8.4:+56371422 | None:intergenic |
| ATGGAGCAATCTTGGAATCT+GGG | 0.486036 | 8.4:-56371341 | MS.gene94064:CDS |
| AGCGTTGTTGAAGAATAAAT+CGG | 0.490943 | 8.4:+56371196 | None:intergenic |
| CATATTAATAGTGCATTTCG+TGG | 0.492792 | 8.4:+56371792 | None:intergenic |
| TCAATGGCTACCTTGAAGGC+TGG | 0.494973 | 8.4:-56371576 | MS.gene94064:CDS |
| GGACTTGGATTTACTGGCCA+TGG | 0.496989 | 8.4:-56371735 | MS.gene94064:CDS |
| GCATGAAATTGTTTGTTGAA+AGG | 0.497147 | 8.4:+56372153 | None:intergenic |
| ATTGGCTTGCAGCATTTGAA+GGG | 0.501471 | 8.4:-56371390 | MS.gene94064:CDS |
| TTGGATTTACTGGCCATGGT+TGG | 0.506088 | 8.4:-56371731 | MS.gene94064:CDS |
| CTTCAAATGCTGCAAGCCAA+TGG | 0.507717 | 8.4:+56371392 | None:intergenic |
| TCATTGGAAATTGAGCATTT+GGG | 0.508386 | 8.4:+56371997 | None:intergenic |
| ATTGATGTTGTGAAGTTGAC+GGG | 0.515309 | 8.4:-56371838 | MS.gene94064:CDS |
| TTATAATGAAGCAGTAGAGT+TGG | 0.517909 | 8.4:+56371307 | None:intergenic |
| CAGGAATCATGATTGGCCAT+TGG | 0.524443 | 8.4:-56371408 | MS.gene94064:CDS |
| TCGCCGTCATGACCCAAAAT+GGG | 0.524963 | 8.4:-56371470 | MS.gene94064:CDS |
| CCTGGTGCGAGGTTAAGCTT+CGG | 0.527503 | 8.4:+56371367 | None:intergenic |
| CTAACATACATCCAGAAGAA+AGG | 0.530527 | 8.4:+56371760 | None:intergenic |
| GGATGGCGCAACAAGCGATA+AGG | 0.531266 | 8.4:+56371217 | None:intergenic |
| TTCAACAAACAATTTCATGC+TGG | 0.532421 | 8.4:-56372150 | MS.gene94064:CDS |
| CCGAAGCTTAACCTCGCACC+AGG | 0.536273 | 8.4:-56371367 | MS.gene94064:CDS |
| CTCACAATAGAGAAACCAAA+AGG | 0.537134 | 8.4:+56372195 | None:intergenic |
| AGGTAGCCATTGAAATAAGC+TGG | 0.540582 | 8.4:+56371586 | None:intergenic |
| TGGATTACTAGAATCTATGC+TGG | 0.542861 | 8.4:+56371972 | None:intergenic |
| TTATAAGCAGAAAGCAGCTA+TGG | 0.543006 | 8.4:-56371098 | MS.gene94064:CDS |
| GAACAAAAGGCGAGACCACA+TGG | 0.550227 | 8.4:+56371435 | None:intergenic |
| CCAAGATTGCTCCATCCACC+TGG | 0.554362 | 8.4:+56371349 | None:intergenic |
| TACAATGAATAGCAGACTCA+AGG | 0.556555 | 8.4:+56371138 | None:intergenic |
| TTAACCTCGCACCAGGTGGA+TGG | 0.576435 | 8.4:-56371360 | MS.gene94064:CDS |
| TGTTGTTTGTTTAAGTAACG+AGG | 0.577958 | 8.4:+56372044 | None:intergenic |
| TGCTCCATCCACCTGGTGCG+AGG | 0.585566 | 8.4:+56371356 | None:intergenic |
| AATTGATGTTGTGAAGTTGA+CGG | 0.587330 | 8.4:-56371839 | MS.gene94064:CDS |
| TTTCGTGGCTGTAGTTATGA+CGG | 0.589274 | 8.4:+56371807 | None:intergenic |
| TAAGCAGAAAGCAGCTATGG+AGG | 0.590622 | 8.4:-56371095 | MS.gene94064:CDS |
| GCAGAAAGCAGCTATGGAGG+AGG | 0.602669 | 8.4:-56371092 | MS.gene94064:CDS |
| GAGTCTGCTATTCATTGTAG+CGG | 0.631042 | 8.4:-56371134 | MS.gene94064:CDS |
| GAAATAAGCTGGTGGAACAA+GGG | 0.631852 | 8.4:+56371597 | None:intergenic |
| AGCATTATAGCCACCAACCA+TGG | 0.636978 | 8.4:+56371718 | None:intergenic |
| ACAATGAATAGCAGACTCAA+GGG | 0.637477 | 8.4:+56371139 | None:intergenic |
| AAGCTTAACCTCGCACCAGG+TGG | 0.645226 | 8.4:-56371364 | MS.gene94064:CDS |
| TAGCCATTGAAATAAGCTGG+TGG | 0.655130 | 8.4:+56371589 | None:intergenic |
| TTGTTGAAGAATAAATCGGA+TGG | 0.662311 | 8.4:+56371200 | None:intergenic |
| TAACACGCTTAGGTCTTGGG+TGG | 0.680734 | 8.4:+56372103 | None:intergenic |
| ATACTTAGCAAGATACAATG+TGG | 0.686391 | 8.4:+56371282 | None:intergenic |
| GGGATGTGAACTTGCCCATG+TGG | 0.690270 | 8.4:-56371450 | MS.gene94064:CDS |
| AACAAAAGGCGAGACCACAT+GGG | 0.690915 | 8.4:+56371436 | None:intergenic |
| TTGATGTTGTGAAGTTGACG+GGG | 0.752016 | 8.4:-56371837 | MS.gene94064:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACTTAAACAAACAACAAAAA+GGG | - | chr8.4:56371206-56371225 | MS.gene94064:CDS | 20.0% |
| !! | TACTTAAACAAACAACAAAA+AGG | - | chr8.4:56371205-56371224 | MS.gene94064:CDS | 20.0% |
| !! | TATACAAAAAAGAAAACCAA+AGG | + | chr8.4:56371693-56371712 | None:intergenic | 20.0% |
| ! | CATAATAATCTCCATATAAC+CGG | + | chr8.4:56371752-56371771 | None:intergenic | 25.0% |
| ! | TAAGTATTCAGAAAAAGTAG+TGG | - | chr8.4:56371977-56371996 | MS.gene94064:CDS | 25.0% |
| !!! | TTTTTGAAGTACTTTAGAAC+TGG | + | chr8.4:56371294-56371313 | None:intergenic | 25.0% |
| AATTGAAAACTAACACGCTT+AGG | + | chr8.4:56371153-56371172 | None:intergenic | 30.0% | |
| AATTGATGTTGTGAAGTTGA+CGG | - | chr8.4:56371404-56371423 | MS.gene94064:CDS | 30.0% | |
| AGCGTTGTTGAAGAATAAAT+CGG | + | chr8.4:56372050-56372069 | None:intergenic | 30.0% | |
| ATACTTAGCAAGATACAATG+TGG | + | chr8.4:56371964-56371983 | None:intergenic | 30.0% | |
| CATATTAATAGTGCATTTCG+TGG | + | chr8.4:56371454-56371473 | None:intergenic | 30.0% | |
| CTATAATGCTTGCAGTTTAT+GGG | - | chr8.4:56371538-56371557 | MS.gene94064:CDS | 30.0% | |
| CTTAACTTTGCTTGCATATT+CGG | + | chr8.4:56372210-56372229 | None:intergenic | 30.0% | |
| GTTTAAGTAACGAGGAAAAA+TGG | + | chr8.4:56371194-56371213 | None:intergenic | 30.0% | |
| TCATTGGAAATTGAGCATTT+GGG | + | chr8.4:56371249-56371268 | None:intergenic | 30.0% | |
| TTATAATGAAGCAGTAGAGT+TGG | + | chr8.4:56371939-56371958 | None:intergenic | 30.0% | |
| TTCAACAAACAATTTCATGC+TGG | - | chr8.4:56371093-56371112 | MS.gene94064:CDS | 30.0% | |
| TTCATTGGAAATTGAGCATT+TGG | + | chr8.4:56371250-56371269 | None:intergenic | 30.0% | |
| TTGTTGAAGAATAAATCGGA+TGG | + | chr8.4:56372046-56372065 | None:intergenic | 30.0% | |
| TTTCAATTGAATCACAGCTA+TGG | - | chr8.4:56371165-56371184 | MS.gene94064:CDS | 30.0% | |
| ! | GCATGAAATTGTTTGTTGAA+AGG | + | chr8.4:56371093-56371112 | None:intergenic | 30.0% |
| ! | TCGTTACATCAACTTTTACA+TGG | - | chr8.4:56371619-56371638 | MS.gene94064:CDS | 30.0% |
| ! | TGTTGTTTGTTTAAGTAACG+AGG | + | chr8.4:56371202-56371221 | None:intergenic | 30.0% |
| !! | TTTATGGGTTATACTGCTTT+TGG | - | chr8.4:56371553-56371572 | MS.gene94064:CDS | 30.0% |
| ACAATGAATAGCAGACTCAA+GGG | + | chr8.4:56372107-56372126 | None:intergenic | 35.0% | |
| ATTGATGTTGTGAAGTTGAC+GGG | - | chr8.4:56371405-56371424 | MS.gene94064:CDS | 35.0% | |
| CTAACATACATCCAGAAGAA+AGG | + | chr8.4:56371486-56371505 | None:intergenic | 35.0% | |
| CTCACAATAGAGAAACCAAA+AGG | + | chr8.4:56371051-56371070 | None:intergenic | 35.0% | |
| GCTATAATGCTTGCAGTTTA+TGG | - | chr8.4:56371537-56371556 | MS.gene94064:CDS | 35.0% | |
| GGCAAACATATTTGAGCAAT+TGG | - | chr8.4:56371593-56371612 | MS.gene94064:CDS | 35.0% | |
| GTCAACTTCACAACATCAAT+TGG | + | chr8.4:56371405-56371424 | None:intergenic | 35.0% | |
| TACAATGAATAGCAGACTCA+AGG | + | chr8.4:56372108-56372127 | None:intergenic | 35.0% | |
| TATTTCAATGGCTACCTTGA+AGG | - | chr8.4:56371663-56371682 | MS.gene94064:CDS | 35.0% | |
| TGCTCTTAAATCCTTTCTTC+TGG | - | chr8.4:56371472-56371491 | MS.gene94064:CDS | 35.0% | |
| TGGATTACTAGAATCTATGC+TGG | + | chr8.4:56371274-56371293 | None:intergenic | 35.0% | |
| TTATAAGCAGAAAGCAGCTA+TGG | - | chr8.4:56372145-56372164 | MS.gene94064:CDS | 35.0% | |
| ! | CACTCAAAAACTGTACCTTT+TGG | - | chr8.4:56371033-56371052 | MS.gene94064:CDS | 35.0% |
| ! | GCTTTAATGGTCGAAACTTT+TGG | - | chr8.4:56372173-56372192 | MS.gene94064:CDS | 35.0% |
| ! | TTTTCTTCAACGTTAGTGTC+CGG | - | chr8.4:56371724-56371743 | MS.gene94064:CDS | 35.0% |
| !! | TTGCATATTCGGTGTATATC+TGG | + | chr8.4:56372199-56372218 | None:intergenic | 35.0% |
| AAACTAACACGCTTAGGTCT+TGG | + | chr8.4:56371147-56371166 | None:intergenic | 40.0% | |
| AACTAACACGCTTAGGTCTT+GGG | + | chr8.4:56371146-56371165 | None:intergenic | 40.0% | |
| AGGTAGCCATTGAAATAAGC+TGG | + | chr8.4:56371660-56371679 | None:intergenic | 40.0% | |
| ATGGAGCAATCTTGGAATCT+GGG | - | chr8.4:56371902-56371921 | MS.gene94064:CDS | 40.0% | |
| CCAAATTGCACAACAGAAGT+TGG | + | chr8.4:56372005-56372024 | None:intergenic | 40.0% | |
| CCAACTTCTGTTGTGCAATT+TGG | - | chr8.4:56372002-56372021 | MS.gene94064:CDS | 40.0% | |
| GAAATAAGCTGGTGGAACAA+GGG | + | chr8.4:56371649-56371668 | None:intergenic | 40.0% | |
| GAAATTGAGCATTTGGGAAG+AGG | + | chr8.4:56371243-56371262 | None:intergenic | 40.0% | |
| GAGTCTGCTATTCATTGTAG+CGG | - | chr8.4:56372109-56372128 | MS.gene94064:CDS | 40.0% | |
| GTTCCACCAGCTTATTTCAA+TGG | - | chr8.4:56371651-56371670 | MS.gene94064:CDS | 40.0% | |
| TAGCCATTGAAATAAGCTGG+TGG | + | chr8.4:56371657-56371676 | None:intergenic | 40.0% | |
| TCTTCTGGATGTATGTTAGC+TGG | - | chr8.4:56371487-56371506 | MS.gene94064:CDS | 40.0% | |
| TGAAATAAGCTGGTGGAACA+AGG | + | chr8.4:56371650-56371669 | None:intergenic | 40.0% | |
| TGATTCCTGCACTGAACAAA+AGG | + | chr8.4:56371824-56371843 | None:intergenic | 40.0% | |
| TTAGCTGGACTTGGATTTAC+TGG | - | chr8.4:56371502-56371521 | MS.gene94064:CDS | 40.0% | |
| TTCAGTGCAGGAATCATGAT+TGG | - | chr8.4:56371828-56371847 | MS.gene94064:CDS | 40.0% | |
| TTGATGTTGTGAAGTTGACG+GGG | - | chr8.4:56371406-56371425 | MS.gene94064:CDS | 40.0% | |
| TTTCGTGGCTGTAGTTATGA+CGG | + | chr8.4:56371439-56371458 | None:intergenic | 40.0% | |
| ! | AATCTCCATATAACCGGTTC+CGG | + | chr8.4:56371746-56371765 | None:intergenic | 40.0% |
| ! | GCAAGTTCACATCCCATTTT+GGG | + | chr8.4:56371788-56371807 | None:intergenic | 40.0% |
| !! | AGAATCTATGCTGGCTTCAT+TGG | + | chr8.4:56371265-56371284 | None:intergenic | 40.0% |
| !! | ATTGGCTTGCAGCATTTGAA+GGG | - | chr8.4:56371853-56371872 | MS.gene94064:CDS | 40.0% |
| !! | TGCTTTTGGAAACACATGCT+TGG | - | chr8.4:56371567-56371586 | MS.gene94064:CDS | 40.0% |
| AACAAAAGGCGAGACCACAT+GGG | + | chr8.4:56371810-56371829 | None:intergenic | 45.0% | |
| AGCATTATAGCCACCAACCA+TGG | + | chr8.4:56371528-56371547 | None:intergenic | 45.0% | |
| CTTCAAATGCTGCAAGCCAA+TGG | + | chr8.4:56371854-56371873 | None:intergenic | 45.0% | |
| GATGGAGCAATCTTGGAATC+TGG | - | chr8.4:56371901-56371920 | MS.gene94064:CDS | 45.0% | |
| GGATGTATGTTAGCTGGACT+TGG | - | chr8.4:56371493-56371512 | MS.gene94064:CDS | 45.0% | |
| TAAGCAGAAAGCAGCTATGG+AGG | - | chr8.4:56372148-56372167 | MS.gene94064:CDS | 45.0% | |
| TTGGAAACACATGCTTGGTC+AGG | - | chr8.4:56371572-56371591 | MS.gene94064:CDS | 45.0% | |
| TTGGATTTACTGGCCATGGT+TGG | - | chr8.4:56371512-56371531 | MS.gene94064:CDS | 45.0% | |
| ! | AGCTATGGAGGAGGCTTTAA+TGG | - | chr8.4:56372160-56372179 | MS.gene94064:CDS | 45.0% |
| ! | CAGGAATCATGATTGGCCAT+TGG | - | chr8.4:56371835-56371854 | MS.gene94064:CDS | 45.0% |
| ! | GGCAAGTTCACATCCCATTT+TGG | + | chr8.4:56371789-56371808 | None:intergenic | 45.0% |
| !! | CATCCCATTTTGGGTCATGA+CGG | + | chr8.4:56371779-56371798 | None:intergenic | 45.0% |
| !! | CATTGGCTTGCAGCATTTGA+AGG | - | chr8.4:56371852-56371871 | MS.gene94064:CDS | 45.0% |
| AAACCAAAGGCCAGCCTTCA+AGG | + | chr8.4:56371680-56371699 | None:intergenic | 50.0% | |
| AGTGTCCGGAACCGGTTATA+TGG | - | chr8.4:56371738-56371757 | MS.gene94064:CDS | 50.0% | |
| GAACAAAAGGCGAGACCACA+TGG | + | chr8.4:56371811-56371830 | None:intergenic | 50.0% | |
| GATTTACTGGCCATGGTTGG+TGG | - | chr8.4:56371515-56371534 | MS.gene94064:CDS | 50.0% | |
| GGACTTGGATTTACTGGCCA+TGG | - | chr8.4:56371508-56371527 | MS.gene94064:CDS | 50.0% | |
| TAACACGCTTAGGTCTTGGG+TGG | + | chr8.4:56371143-56371162 | None:intergenic | 50.0% | |
| TCAACGTTAGTGTCCGGAAC+CGG | - | chr8.4:56371730-56371749 | MS.gene94064:CDS | 50.0% | |
| TCAATGGCTACCTTGAAGGC+TGG | - | chr8.4:56371667-56371686 | MS.gene94064:CDS | 50.0% | |
| TCGCCGTCATGACCCAAAAT+GGG | - | chr8.4:56371773-56371792 | MS.gene94064:CDS | 50.0% | |
| ! | TCTCGCCTTTTGTTCAGTGC+AGG | - | chr8.4:56371816-56371835 | MS.gene94064:CDS | 50.0% |
| AAGCTTAACCTCGCACCAGG+TGG | - | chr8.4:56371879-56371898 | MS.gene94064:CDS | 55.0% | |
| CCAAGATTGCTCCATCCACC+TGG | + | chr8.4:56371897-56371916 | None:intergenic | 55.0% | |
| CCAGGTGGATGGAGCAATCT+TGG | - | chr8.4:56371894-56371913 | MS.gene94064:CDS | 55.0% | |
| CCTGGTGCGAGGTTAAGCTT+CGG | + | chr8.4:56371879-56371898 | None:intergenic | 55.0% | |
| CTCGCCGTCATGACCCAAAA+TGG | - | chr8.4:56371772-56371791 | MS.gene94064:CDS | 55.0% | |
| GCAGAAAGCAGCTATGGAGG+AGG | - | chr8.4:56372151-56372170 | MS.gene94064:CDS | 55.0% | |
| GGATGGCGCAACAAGCGATA+AGG | + | chr8.4:56372029-56372048 | None:intergenic | 55.0% | |
| GGGATGTGAACTTGCCCATG+TGG | - | chr8.4:56371793-56371812 | MS.gene94064:CDS | 55.0% | |
| TTAACCTCGCACCAGGTGGA+TGG | - | chr8.4:56371883-56371902 | MS.gene94064:CDS | 55.0% | |
| !! | CTACCTTGAAGGCTGGCCTT+TGG | - | chr8.4:56371674-56371693 | MS.gene94064:CDS | 55.0% |
| CCGAAGCTTAACCTCGCACC+AGG | - | chr8.4:56371876-56371895 | MS.gene94064:CDS | 60.0% | |
| TGCTCCATCCACCTGGTGCG+AGG | + | chr8.4:56371890-56371909 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 56371009 | 56372256 | 56371009 | ID=MS.gene94064 |
| chr8.4 | mRNA | 56371009 | 56372256 | 56371009 | ID=MS.gene94064.t1;Parent=MS.gene94064 |
| chr8.4 | exon | 56371009 | 56372256 | 56371009 | ID=MS.gene94064.t1.exon1;Parent=MS.gene94064.t1 |
| chr8.4 | CDS | 56371009 | 56372256 | 56371009 | ID=cds.MS.gene94064.t1;Parent=MS.gene94064.t1 |
| Gene Sequence |
| Protein sequence |
