AlfalfaGEDB | MsG0280006376.01

M. sativa cultivar ZhongmuNo.1 / MsG0280006376.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006376.01.T01	XP_003593045.1	97.959	147	3	0	1	147	1	147	4.18E-97	286

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006376.01.T01	A2Q3U1	97.959	147	3	0	1	147	1	147	2.00e-97	286

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047814.01	MsG0280006376.01	0.801319	9.521858e-49	4.533728e-46
MsG0080048383.01	MsG0280006376.01	0.834831	2.387278e-56	2.866595e-53
MsG0080048687.01	MsG0280006376.01	0.804803	1.812642e-49	9.434965e-47
MsG0080048971.01	MsG0280006376.01	0.807889	4.054658e-50	2.284950e-47
MsG0180000023.01	MsG0280006376.01	0.808417	3.129138e-50	1.788210e-47
MsG0180000331.01	MsG0280006376.01	0.855678	5.371236e-62	1.250035e-58
MsG0180000824.01	MsG0280006376.01	0.835127	2.010569e-56	2.435562e-53
MsG0180000836.01	MsG0280006376.01	0.807450	5.023975e-50	2.799258e-47
MsG0180003651.01	MsG0280006376.01	0.818457	1.940347e-52	1.453331e-49
MsG0180003793.01	MsG0280006376.01	0.826886	2.120390e-54	2.014292e-51
MsG0180004398.01	MsG0280006376.01	0.812123	4.966718e-51	3.131163e-48
MsG0180004854.01	MsG0280006376.01	0.821924	3.116865e-53	2.570819e-50
MsG0180005371.01	MsG0280006376.01	0.809937	1.477732e-50	8.791671e-48
MsG0280006376.01	MsG0280006545.01	0.827395	1.602178e-54	1.544984e-51
MsG0280006376.01	MsG0280006596.01	0.813964	1.960371e-51	1.298581e-48
MsG0280006376.01	MsG0280006993.01	0.813210	2.873012e-51	1.864469e-48
MsG0280006376.01	MsG0280007226.01	0.804559	2.038509e-49	1.054315e-46
MsG0280006376.01	MsG0280007491.01	0.827524	1.492139e-54	1.444037e-51
MsG0280006376.01	MsG0280007903.01	0.802302	5.984482e-49	2.921485e-46
MsG0280006376.01	MsG0280008004.01	0.807173	5.751435e-50	3.181477e-47
MsG0280006376.01	MsG0280008885.01	0.802050	6.742973e-49	3.270538e-46
MsG0280006376.01	MsG0280010002.01	0.825148	5.488587e-54	4.962082e-51
MsG0280006376.01	MsG0280010489.01	0.808391	3.169003e-50	1.809791e-47
MsG0280006376.01	MsG0280011014.01	0.800130	1.664628e-48	7.690690e-46
MsG0280006376.01	MsG0280011304.01	0.818784	1.635965e-52	1.236331e-49
MsG0280006376.01	MsG0280011388.01	0.831264	1.841894e-55	1.988348e-52
MsG0280006376.01	MsG0380011747.01	0.801166	1.023664e-48	4.855320e-46
MsG0280006376.01	MsG0380015435.01	0.816337	5.824878e-52	4.116247e-49
MsG0280006376.01	MsG0380016290.01	0.811831	5.750895e-51	3.596915e-48
MsG0280006376.01	MsG0380016389.01	0.810938	8.986979e-51	5.490498e-48
MsG0280006376.01	MsG0380016505.01	0.811753	5.980408e-51	3.732786e-48
MsG0280006376.01	MsG0380016765.01	0.813798	2.132380e-51	1.406209e-48
MsG0280006376.01	MsG0480018278.01	0.802145	6.446820e-49	3.134454e-46
MsG0280006376.01	MsG0480018477.01	0.813569	2.395445e-51	1.569886e-48
MsG0280006376.01	MsG0480018641.01	0.801697	7.966742e-49	3.829469e-46
MsG0280006376.01	MsG0480018894.01	0.803976	2.696123e-49	1.373762e-46
MsG0280006376.01	MsG0480021077.01	-0.839305	1.716037e-57	2.360105e-54
MsG0280006376.01	MsG0480021448.01	0.801926	7.148556e-49	3.456341e-46
MsG0280006376.01	MsG0480021464.01	0.815608	8.473366e-52	5.869638e-49
MsG0280006376.01	MsG0480021768.01	0.828256	9.946021e-55	9.833307e-52
MsG0280006376.01	MsG0480022780.01	0.818551	1.847583e-52	1.387313e-49
MsG0280006376.01	MsG0480022825.01	0.811991	5.306316e-51	3.333274e-48
MsG0280006376.01	MsG0480023221.01	0.830279	3.211628e-55	3.368063e-52
MsG0280006376.01	MsG0480023272.01	0.823470	1.361308e-53	1.173158e-50
MsG0280006376.01	MsG0480023307.01	0.808511	2.987707e-50	1.711556e-47
MsG0280006376.01	MsG0680031145.01	0.818392	2.007352e-52	1.500770e-49
MsG0280006376.01	MsG0680031628.01	0.812667	3.777493e-51	2.416350e-48
MsG0280006376.01	MsG0680034671.01	0.825831	3.782533e-54	3.486588e-51
MsG0280006376.01	MsG0680035888.01	0.800603	1.333963e-48	6.237276e-46
MsG0280006376.01	MsG0780036950.01	0.816637	4.989442e-52	3.554727e-49
MsG0280006376.01	MsG0780037093.01	0.820184	7.843696e-53	6.163025e-50
MsG0280006376.01	MsG0780037240.01	0.808488	3.022351e-50	1.730368e-47
MsG0280006376.01	MsG0780038400.01	0.805847	1.095368e-49	5.856499e-47
MsG0280006376.01	MsG0780038907.01	0.815148	1.072514e-51	7.335423e-49
MsG0280006376.01	MsG0780039307.01	0.800578	1.349272e-48	6.305045e-46
MsG0280006376.01	MsG0780039643.01	0.839371	1.648927e-57	2.272576e-54
MsG0280006376.01	MsG0780039828.01	0.806765	7.016079e-50	3.840128e-47
MsG0280006376.01	MsG0780039845.01	0.800900	1.160074e-48	5.465607e-46
MsG0280006376.01	MsG0780040926.01	0.818314	2.091549e-52	1.560230e-49
MsG0280006376.01	MsG0780041016.01	0.808262	3.376207e-50	1.921644e-47
MsG0280006376.01	MsG0780041223.01	0.818476	1.921922e-52	1.440191e-49
MsG0280006376.01	MsG0880042128.01	0.830914	2.245174e-55	2.398990e-52
MsG0280006376.01	MsG0880042129.01	0.823919	1.068500e-53	9.326447e-51
MsG0280006376.01	MsG0880042795.01	0.814537	1.464496e-51	9.853230e-49
MsG0280006376.01	MsG0880042991.01	0.800939	1.138830e-48	5.370779e-46
MsG0280006376.01	MsG0880043749.01	0.802700	4.954396e-49	2.443191e-46
MsG0280006376.01	MsG0880045965.01	0.805906	1.064433e-49	5.699612e-47
MsG0280006376.01	MsG0880046074.01	0.850354	1.792956e-60	3.502266e-57

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006376.01.T01	MTR_2g007190	97.959	147	3	0	1	147	1	147	5.06e-101	286

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006376.01.T01	AT1G60870	59.184	147	60	0	1	147	1	147	1.64e-33	115

Find 26 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGCTGAGAAAGCAAGAAATA+TGG	0.282094	2:-1033402	MsG0280006376.01.T01:CDS
GCTTGGAAAGGAATTTCTTC+TGG	0.317736	2:-1033293	MsG0280006376.01.T01:CDS
AGCAGCTGCCCATGCTTTGT+AGG	0.349595	2:+1033586	None:intergenic
AAGAAATATGGGAAACTTGA+TGG	0.389367	2:-1033390	MsG0280006376.01.T01:CDS
GTGCCATGGATGAATTTAGA+AGG	0.394252	2:-1033481	MsG0280006376.01.T01:CDS
GGAATATCTCGACTCGGCTA+TGG	0.422650	2:-1033507	MsG0280006376.01.T01:CDS
GAGGATTTCAAGAGATGAAG+TGG	0.462068	2:-1033441	MsG0280006376.01.T01:CDS
GCTGAGAAAGCAAGAAATAT+GGG	0.463308	2:-1033401	MsG0280006376.01.T01:CDS
ATAAGATCTTCGATCGTCGA+TGG	0.506443	2:+1033629	None:intergenic
GAAAGGAATTTCTTCTGGCA+AGG	0.513277	2:-1033288	MsG0280006376.01.T01:CDS
AGATAGAATCCTACAAAGCA+TGG	0.513381	2:-1033595	MsG0280006376.01.T01:CDS
AAAGGAAGGTGAACTGAGAA+AGG	0.516993	2:+1033684	None:intergenic
CTGCTTCTATGAAATCTGCT+TGG	0.529588	2:-1033310	MsG0280006376.01.T01:CDS
GGTTTGAAGAAGAACTTGAG+AGG	0.547329	2:-1033460	MsG0280006376.01.T01:CDS
AAACCTTCTAAATTCATCCA+TGG	0.552800	2:+1033478	None:intergenic
TAAAGATTGATCTGAAAGGA+AGG	0.570141	2:+1033670	None:intergenic
AAAGTATATATGTATCAAGA+TGG	0.576287	2:+1033260	None:intergenic
CTCGGCTATGGAAAGTGCCA+TGG	0.577831	2:-1033495	MsG0280006376.01.T01:CDS
TCTATGAAATCTGCTTGGAA+AGG	0.578134	2:-1033305	MsG0280006376.01.T01:CDS
CATGTAAAGATTGATCTGAA+AGG	0.594535	2:+1033666	None:intergenic
AGCCGAGTCGAGATATTCCT+CGG	0.604035	2:+1033511	None:intergenic
TGAACTTGAGCAAGAAAGAG+AGG	0.624850	2:-1033564	MsG0280006376.01.T01:CDS
AGCCGAGGAATATCTCGACT+CGG	0.631008	2:-1033513	MsG0280006376.01.T01:CDS
GATAGAATCCTACAAAGCAT+GGG	0.638623	2:-1033594	MsG0280006376.01.T01:CDS
AGATGCAATGCAAGAAGCCG+AGG	0.640190	2:-1033528	MsG0280006376.01.T01:CDS
ACTTGAGCAAGAAAGAGAGG+TGG	0.641638	2:-1033561	MsG0280006376.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAACCTTCTAAATTCATCCA+TGG	+	Chr2:1033494-1033513	None:intergenic	30.0%
	AAGAAATATGGGAAACTTGA+TGG	-	Chr2:1033579-1033598	MsG0280006376.01.T01:CDS	30.0%
	CATGTAAAGATTGATCTGAA+AGG	+	Chr2:1033306-1033325	None:intergenic	30.0%
	TAAAGATTGATCTGAAAGGA+AGG	+	Chr2:1033302-1033321	None:intergenic	30.0%
	AGATAGAATCCTACAAAGCA+TGG	-	Chr2:1033374-1033393	MsG0280006376.01.T01:CDS	35.0%
	AGCTGAGAAAGCAAGAAATA+TGG	-	Chr2:1033567-1033586	MsG0280006376.01.T01:CDS	35.0%
	GATAGAATCCTACAAAGCAT+GGG	-	Chr2:1033375-1033394	MsG0280006376.01.T01:CDS	35.0%
	GCTGAGAAAGCAAGAAATAT+GGG	-	Chr2:1033568-1033587	MsG0280006376.01.T01:CDS	35.0%
	TCTATGAAATCTGCTTGGAA+AGG	-	Chr2:1033664-1033683	MsG0280006376.01.T01:CDS	35.0%
	AAAGGAAGGTGAACTGAGAA+AGG	+	Chr2:1033288-1033307	None:intergenic	40.0%
	ATAAGATCTTCGATCGTCGA+TGG	+	Chr2:1033343-1033362	None:intergenic	40.0%
	CTGCTTCTATGAAATCTGCT+TGG	-	Chr2:1033659-1033678	MsG0280006376.01.T01:CDS	40.0%
	GAAAGGAATTTCTTCTGGCA+AGG	-	Chr2:1033681-1033700	MsG0280006376.01.T01:CDS	40.0%
	GAGGATTTCAAGAGATGAAG+TGG	-	Chr2:1033528-1033547	MsG0280006376.01.T01:CDS	40.0%
	GCTTGGAAAGGAATTTCTTC+TGG	-	Chr2:1033676-1033695	MsG0280006376.01.T01:CDS	40.0%
	GGTTTGAAGAAGAACTTGAG+AGG	-	Chr2:1033509-1033528	MsG0280006376.01.T01:CDS	40.0%
	GTGCCATGGATGAATTTAGA+AGG	-	Chr2:1033488-1033507	MsG0280006376.01.T01:CDS	40.0%
	TGAACTTGAGCAAGAAAGAG+AGG	-	Chr2:1033405-1033424	MsG0280006376.01.T01:CDS	40.0%
	ACTTGAGCAAGAAAGAGAGG+TGG	-	Chr2:1033408-1033427	MsG0280006376.01.T01:CDS	45.0%
	AGATGCAATGCAAGAAGCCG+AGG	-	Chr2:1033441-1033460	MsG0280006376.01.T01:CDS	50.0%
	AGCCGAGGAATATCTCGACT+CGG	-	Chr2:1033456-1033475	MsG0280006376.01.T01:CDS	50.0%
	AGCCGAGTCGAGATATTCCT+CGG	+	Chr2:1033461-1033480	None:intergenic	50.0%
!!	GGAATATCTCGACTCGGCTA+TGG	-	Chr2:1033462-1033481	MsG0280006376.01.T01:CDS	50.0%
	AGCAGCTGCCCATGCTTTGT+AGG	+	Chr2:1033386-1033405	None:intergenic	55.0%
!!	CTCGGCTATGGAAAGTGCCA+TGG	-	Chr2:1033474-1033493	MsG0280006376.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	1033274	1033717	1033274	ID=MsG0280006376.01;Name=MsG0280006376.01
Chr2	mRNA	1033274	1033717	1033274	ID=MsG0280006376.01.T01;Parent=MsG0280006376.01;Name=MsG0280006376.01.T01;_AED=0.30;_eAED=0.30;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|147
Chr2	exon	1033274	1033717	1033274	ID=MsG0280006376.01.T01:exon:1041;Parent=MsG0280006376.01.T01
Chr2	CDS	1033274	1033717	1033274	ID=MsG0280006376.01.T01:cds;Parent=MsG0280006376.01.T01

Gene Sequence

>MsG0280006376.01.T01

ATGGAAGCTCTCCTTTCTCAGTTCACCTTCCTTTCAGATCAATCTTTACATGACAAGAGCTTCGATCCATCGACGATCGAAGATCTTATGAAACTATTTGAGATAGAATCCTACAAAGCATGGGCAGCTGCTGAACTTGAGCAAGAAAGAGAGGTGGAAGAAGCAGAAGATGCAATGCAAGAAGCCGAGGAATATCTCGACTCGGCTATGGAAAGTGCCATGGATGAATTTAGAAGGTTTGAAGAAGAACTTGAGAGGATTTCAAGAGATGAAGTGGAGAGTTTAGTTCAAACAGCTGAGAAAGCAAGAAATATGGGAAACTTGATGGAAAAAACAGCTTCTATTGCTTCTAAGAAATATATTGAAGCTGCACTTAATTCAGCTACTGCTTCTATGAAATCTGCTTGGAAAGGAATTTCTTCTGGCAAGGTTCATCCATCTTGA

Protein sequence

>MsG0280006376.01.T01

MEALLSQFTFLSDQSLHDKSFDPSTIEDLMKLFEIESYKAWAAAELEQEREVEEAEDAMQEAEEYLDSAMESAMDEFRRFEEELERISRDEVESLVQTAEKARNMGNLMEKTASIASKKYIEAALNSATASMKSAWKGISSGKVHPS*