AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880042128.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042128.01.T01 MTR_8g012380 100.000 63 0 0 8 70 89 151 1.63e-40 130
MsG0880042128.01.T01 MTR_8g012340 56.250 48 21 0 8 55 97 144 1.38e-11 58.2
MsG0880042128.01.T01 MTR_7g053470 54.167 48 22 0 8 55 91 138 2.09e-11 57.8
MsG0880042128.01.T01 MTR_8g080980 55.319 47 21 0 8 54 95 141 5.84e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042128.01.T01 AT3G25220 77.778 63 14 0 8 70 91 153 1.04e-29 102
MsG0880042128.01.T01 AT5G48580 90.385 52 5 0 8 59 91 142 1.80e-29 102
MsG0880042128.01.T01 AT3G25230 59.574 47 19 0 8 54 96 142 3.10e-12 60.1
MsG0880042128.01.T01 AT3G25230 59.574 47 19 0 8 54 96 142 3.16e-12 60.1
MsG0880042128.01.T01 AT5G48570 56.250 48 21 0 8 55 104 151 3.06e-11 57.4

Find 36 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAGATGTATTTAATTTAT+AGG 0.174496 8:+4155529 None:intergenic
TTTAGACAAATCAAAATTAA+TGG 0.209015 8:-4155673 MsG0880042128.01.T01:exon
ATAAATTAAATACATCTATT+TGG 0.214425 8:-4155526 MsG0880042128.01.T01:three_prime_UTR
TTGCTCCTCCTCTTCTTTGC+AGG 0.258188 8:-4156610 MsG0880042128.01.T01:CDS
AGGATGGGACCAAGGATTAT+TGG 0.270076 8:-4156590 MsG0880042128.01.T01:CDS
CAAGCTCGGTGTCAAATATA+AGG 0.271808 8:+4155744 None:intergenic
TGGTAATGAAAGCATTATTA+AGG 0.297295 8:+4155606 None:intergenic
ATAGATGTATTTAATTTATA+GGG 0.299832 8:+4155530 None:intergenic
TTAATGGAAGACTAGACTTT+TGG 0.305728 8:-4155657 MsG0880042128.01.T01:three_prime_UTR
GGATGGGACCAAGGATTATT+GGG 0.407821 8:-4156589 MsG0880042128.01.T01:CDS
GTGATAAATACCTGGAATAG+TGG 0.410355 8:+4156495 None:intergenic
CCTCCTCTTCTTTGCAGGAT+GGG 0.415800 8:-4156605 MsG0880042128.01.T01:CDS
TCCTCCTCTTCTTTGCAGGA+TGG 0.418546 8:-4156606 MsG0880042128.01.T01:CDS
GGTTCCCCACCCACTATTCC+AGG 0.429923 8:-4156505 MsG0880042128.01.T01:intron
AAAACTTGGCTATGGAGAGC+AGG 0.467684 8:-4156527 MsG0880042128.01.T01:CDS
GTCGTTCACTCCCACAAGCT+CGG 0.479973 8:+4155730 None:intergenic
TTAATTTCTTGCAATTGAGT+TGG 0.481555 8:-4155449 None:intergenic
CTGAAAATACCAGCAAAACT+TGG 0.484203 8:-4156541 MsG0880042128.01.T01:CDS
ATATTTGACACCGAGCTTGT+GGG 0.490987 8:-4155740 MsG0880042128.01.T01:CDS
ATACCAGCAAAACTTGGCTA+TGG 0.519186 8:-4156535 MsG0880042128.01.T01:CDS
TATATTTGACACCGAGCTTG+TGG 0.522619 8:-4155741 MsG0880042128.01.T01:CDS
TTCTTTGCAGGATGGGACCA+AGG 0.523846 8:-4156598 MsG0880042128.01.T01:CDS
AAGCTCGGTGTCAAATATAA+GGG 0.529495 8:+4155745 None:intergenic
GACACATTCCCAATAATCCT+TGG 0.533580 8:+4156581 None:intergenic
AATACCTGGAATAGTGGGTG+GGG 0.552424 8:+4156501 None:intergenic
TAATAATGCTTTCATTACCA+AGG 0.566466 8:-4155603 MsG0880042128.01.T01:three_prime_UTR
GGATTATTGGGAATGTGTCT+TGG 0.579154 8:-4156577 MsG0880042128.01.T01:CDS
AGAGTTAGACAATGAAACCT+TGG 0.585805 8:+4155586 None:intergenic
AAATACCTGGAATAGTGGGT+GGG 0.603802 8:+4156500 None:intergenic
CCCATCCTGCAAAGAAGAGG+AGG 0.607323 8:+4156605 None:intergenic
AAACTTGGCTATGGAGAGCA+GGG 0.608629 8:-4156526 MsG0880042128.01.T01:CDS
TGATAAATACCTGGAATAGT+GGG 0.608996 8:+4156496 None:intergenic
GGTCCCATCCTGCAAAGAAG+AGG 0.613698 8:+4156602 None:intergenic
TAAATACCTGGAATAGTGGG+TGG 0.614273 8:+4156499 None:intergenic
TTTCATTGTCTAACTCTGTG+AGG 0.662564 8:-4155580 MsG0880042128.01.T01:three_prime_UTR
GAATGTGTCTTGGTGAGAAG+CGG 0.678965 8:-4156567 MsG0880042128.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATTAAATACATCTATT+TGG - Chr8:4156534-4156553 MsG0880042128.01.T01:CDS 10.0%
!! ATTAATAAATTTAGTTAGTT+AGG + Chr8:4156239-4156258 None:intergenic 10.0%
!! TAACTAAATTTATTAATGTA+TGG - Chr8:4156242-4156261 MsG0880042128.01.T01:intron 10.0%
!!! AATAGATGTATTTAATTTAT+AGG + Chr8:4156534-4156553 None:intergenic 10.0%
!!! ATAGATGTATTTAATTTATA+GGG + Chr8:4156533-4156552 None:intergenic 10.0%
!! AAATTTATTAATGTATGGTT+AGG - Chr8:4156247-4156266 MsG0880042128.01.T01:intron 15.0%
!! AATTTATTAATGTATGGTTA+GGG - Chr8:4156248-4156267 MsG0880042128.01.T01:intron 15.0%
!! GTAAAACAATGTTAAAATAA+CGG - Chr8:4156156-4156175 MsG0880042128.01.T01:intron 15.0%
!! TTTAGACAAATCAAAATTAA+TGG - Chr8:4156387-4156406 MsG0880042128.01.T01:intron 15.0%
!! AATAAATTTCTCAAAATAGC+AGG - Chr8:4156134-4156153 MsG0880042128.01.T01:intron 20.0%
!!! AAAATAATCTCATTTTGTGA+AGG - Chr8:4156082-4156101 MsG0880042128.01.T01:intron 20.0%
!!! ATTTTGATTTGTCTAAAGTT+CGG + Chr8:4156384-4156403 None:intergenic 20.0%
!!! CTGATTTACATTATTAGTTT+TGG - Chr8:4155769-4155788 MsG0880042128.01.T01:intron 20.0%
!!! GATTATTTTGTATTCAATGT+CGG + Chr8:4156071-4156090 None:intergenic 20.0%
! AAGCAATACATGCTAAAAAA+TGG + Chr8:4156198-4156217 None:intergenic 25.0%
! ACTAACTTGAAATATGAAAG+AGG + Chr8:4155633-4155652 None:intergenic 25.0%
! AGCAATACATGCTAAAAAAT+GGG + Chr8:4156197-4156216 None:intergenic 25.0%
! CTAAATAAACTCATAATACG+AGG + Chr8:4156111-4156130 None:intergenic 25.0%
! GAAAAGTTATGTGAGTAATA+TGG + Chr8:4155889-4155908 None:intergenic 25.0%
! GTGAGTAATATGGTATTAAA+CGG + Chr8:4155879-4155898 None:intergenic 25.0%
! TAATAATGCTTTCATTACCA+AGG - Chr8:4156457-4156476 MsG0880042128.01.T01:intron 25.0%
!! AATGTTCTTTTATGTCAATG+TGG - Chr8:4155847-4155866 MsG0880042128.01.T01:intron 25.0%
!! TATGCCACTTAAAATATTAG+TGG - Chr8:4155813-4155832 MsG0880042128.01.T01:intron 25.0%
!! TTGAAATATGAAAGAGGTTT+TGG + Chr8:4155627-4155646 None:intergenic 25.0%
!!! TCTTCCACTAATATTTTAAG+TGG + Chr8:4155820-4155839 None:intergenic 25.0%
!!! TGGTAATGAAAGCATTATTA+AGG + Chr8:4156457-4156476 None:intergenic 25.0%
!!! TGGTTTTGATTATCTGTTTT+AGG - Chr8:4155789-4155808 MsG0880042128.01.T01:intron 25.0%
!!! TTTTAGCATGTATTGCTTTT+CGG - Chr8:4156200-4156219 MsG0880042128.01.T01:intron 25.0%
ATACATGCTAAAAAATGGGA+TGG + Chr8:4156193-4156212 None:intergenic 30.0%
ATATCCAACAGCTAAATATC+TGG - Chr8:4155660-4155679 MsG0880042128.01.T01:three_prime_UTR 30.0%
ATATCCAGATATTTAGCTGT+TGG + Chr8:4155667-4155686 None:intergenic 30.0%
CAACAATTGTGATTGATTGA+AGG + Chr8:4155957-4155976 None:intergenic 30.0%
TGATAAATACCTGGAATAGT+GGG + Chr8:4155567-4155586 None:intergenic 30.0%
TTACTAACGTGATAAATACC+TGG + Chr8:4155576-4155595 None:intergenic 30.0%
! ATAATCTCATTTTGTGAAGG+AGG - Chr8:4156085-4156104 MsG0880042128.01.T01:intron 30.0%
! ATGTCGGAGTAAAAATGTTT+TGG + Chr8:4156055-4156074 None:intergenic 30.0%
! TTAATGGAAGACTAGACTTT+TGG - Chr8:4156403-4156422 MsG0880042128.01.T01:intron 30.0%
! TTACTCACATAACTTTTCGA+AGG - Chr8:4155891-4155910 MsG0880042128.01.T01:intron 30.0%
AATATGGCAGTTGAGGAAAA+CGG + Chr8:4155690-4155709 None:intergenic 35.0%
AGAGTTAGACAATGAAACCT+TGG + Chr8:4156477-4156496 None:intergenic 35.0%
ATACCTTACAAGTCATACAG+TGG - Chr8:4156008-4156027 MsG0880042128.01.T01:intron 35.0%
CTGAAAATACCAGCAAAACT+TGG - Chr8:4155519-4155538 MsG0880042128.01.T01:three_prime_UTR 35.0%
GTGATAAATACCTGGAATAG+TGG + Chr8:4155568-4155587 None:intergenic 35.0%
TACAGTGGATACTTTGTGAT+TGG - Chr8:4156023-4156042 MsG0880042128.01.T01:intron 35.0%
TACCAAACTGTAGACCAATA+TGG + Chr8:4155706-4155725 None:intergenic 35.0%
TTTCATTGTCTAACTCTGTG+AGG - Chr8:4156480-4156499 MsG0880042128.01.T01:intron 35.0%
! GCTTTCGTGAGAATTTTCAT+TGG - Chr8:4156576-4156595 MsG0880042128.01.T01:CDS 35.0%
! GGTAGACGAGTATTTTATCT+TGG - Chr8:4155722-4155741 MsG0880042128.01.T01:CDS 35.0%
! TATCCACTGTATGACTTGTA+AGG + Chr8:4156014-4156033 None:intergenic 35.0%
!! AAGCTCGGTGTCAAATATAA+GGG + Chr8:4156318-4156337 None:intergenic 35.0%
ATACCAGCAAAACTTGGCTA+TGG - Chr8:4155525-4155544 MsG0880042128.01.T01:three_prime_UTR 40.0%
ATATTTGACACCGAGCTTGT+GGG - Chr8:4156320-4156339 MsG0880042128.01.T01:intron 40.0%
GACACATTCCCAATAATCCT+TGG + Chr8:4155482-4155501 None:intergenic 40.0%
GGATTATTGGGAATGTGTCT+TGG - Chr8:4155483-4155502 MsG0880042128.01.T01:three_prime_UTR 40.0%
TATATTTGACACCGAGCTTG+TGG - Chr8:4156319-4156338 MsG0880042128.01.T01:intron 40.0%
! GTTTTCCTCAACTGCCATAT+TGG - Chr8:4155689-4155708 MsG0880042128.01.T01:exon 40.0%
! TAAATACCTGGAATAGTGGG+TGG + Chr8:4155564-4155583 None:intergenic 40.0%
! TGCCATATTGGTCTACAGTT+TGG - Chr8:4155701-4155720 MsG0880042128.01.T01:CDS 40.0%
!! AAATACCTGGAATAGTGGGT+GGG + Chr8:4155563-4155582 None:intergenic 40.0%
!! CAAGCTCGGTGTCAAATATA+AGG + Chr8:4156319-4156338 None:intergenic 40.0%
AAAACTTGGCTATGGAGAGC+AGG - Chr8:4155533-4155552 MsG0880042128.01.T01:three_prime_UTR 45.0%
AAACTTGGCTATGGAGAGCA+GGG - Chr8:4155534-4155553 MsG0880042128.01.T01:three_prime_UTR 45.0%
AGGATGGGACCAAGGATTAT+TGG - Chr8:4155470-4155489 MsG0880042128.01.T01:three_prime_UTR 45.0%
GAATGTGTCTTGGTGAGAAG+CGG - Chr8:4155493-4155512 MsG0880042128.01.T01:three_prime_UTR 45.0%
GGATGGGACCAAGGATTATT+GGG - Chr8:4155471-4155490 MsG0880042128.01.T01:three_prime_UTR 45.0%
GTAGACCAATATGGCAGTTG+AGG + Chr8:4155697-4155716 None:intergenic 45.0%
! TCTCCATAGCCAAGTTTTGC+TGG + Chr8:4155531-4155550 None:intergenic 45.0%
!! AATACCTGGAATAGTGGGTG+GGG + Chr8:4155562-4155581 None:intergenic 45.0%
!! TGACATTGACTTGAACCTGC+AGG - Chr8:4156284-4156303 MsG0880042128.01.T01:intron 45.0%
CCTCCTCTTCTTTGCAGGAT+GGG - Chr8:4155455-4155474 MsG0880042128.01.T01:three_prime_UTR 50.0%
TCCTCCTCTTCTTTGCAGGA+TGG - Chr8:4155454-4155473 MsG0880042128.01.T01:three_prime_UTR 50.0%
TTCTTTGCAGGATGGGACCA+AGG - Chr8:4155462-4155481 MsG0880042128.01.T01:three_prime_UTR 50.0%
TTGCTCCTCCTCTTCTTTGC+AGG - Chr8:4155450-4155469 MsG0880042128.01.T01:three_prime_UTR 50.0%
!! CATTGACTTGAACCTGCAGG+TGG - Chr8:4156287-4156306 MsG0880042128.01.T01:intron 50.0%
CCCATCCTGCAAAGAAGAGG+AGG + Chr8:4155458-4155477 None:intergenic 55.0%
GGTCCCATCCTGCAAAGAAG+AGG + Chr8:4155461-4155480 None:intergenic 55.0%
GTCGTTCACTCCCACAAGCT+CGG + Chr8:4156333-4156352 None:intergenic 55.0%
ATAAGGGTCGCACCACCTGC+AGG + Chr8:4156302-4156321 None:intergenic 60.0%
GGTTCCCCACCCACTATTCC+AGG - Chr8:4155555-4155574 MsG0880042128.01.T01:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 4155450 4156632 4155450 ID=MsG0880042128.01;Name=MsG0880042128.01
Chr8 mRNA 4155450 4156632 4155450 ID=MsG0880042128.01.T01;Parent=MsG0880042128.01;Name=MsG0880042128.01.T01;_AED=0.50;_eAED=0.52;_QI=0|1|0.5|1|1|1|2|241|70
Chr8 exon 4155450 4155776 4155450 ID=MsG0880042128.01.T01:exon:2471;Parent=MsG0880042128.01.T01
Chr8 exon 4156506 4156632 4156506 ID=MsG0880042128.01.T01:exon:2470;Parent=MsG0880042128.01.T01
Chr8 CDS 4156506 4156632 4156506 ID=MsG0880042128.01.T01:cds;Parent=MsG0880042128.01.T01
Chr8 CDS 4155691 4155776 4155691 ID=MsG0880042128.01.T01:cds;Parent=MsG0880042128.01.T01
Chr8 three_prime_UTR 4155450 4155690 4155450 ID=MsG0880042128.01.T01:three_prime_utr;Parent=MsG0880042128.01.T01
Gene Sequence

>MsG0880042128.01.T01

TTGCTCCTCCTCTTCTTTGCAGGATGGGACCAAGGATTATTGGGAATGTGTCTTGGTGAGAAGCGGAAACTGAAAATACCAGCAAAACTTGGCTATGGAGAGCAGGGTTCCCCACCCACTATTCCAGGTGGTGCGACCCTTATATTTGACACCGAGCTTGTGGGAGTGAACGACAAAAGTTTAAGCGAAGAAAAATCAAACTCCGAACTTTAG

Protein sequence

>MsG0880042128.01.T01

LLLLFFAGWDQGLLGMCLGEKRKLKIPAKLGYGEQGSPPTIPGGATLIFDTELVGVNDKSLSEEKSNSEL*