AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780036950.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036950.01.T01 MTR_7g024350 91.071 56 5 0 4 59 1 56 3.52e-32 107
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036950.01.T01 AT1G06515 77.419 31 7 0 4 34 1 31 4.38e-12 56.2
MsG0780036950.01.T01 AT1G06515 77.419 31 7 0 4 34 1 31 4.38e-12 56.2
MsG0780036950.01.T01 AT2G30942 77.419 31 7 0 4 34 1 31 6.92e-12 55.8

Find 19 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTAACTATTACAGATATTT+TGG 0.137656 7:+17048861 MsG0780036950.01.T01:intron
CACCTTTACAATGTTACTTT+TGG 0.153503 7:+17048539 MsG0780036950.01.T01:CDS
TCATTCATCTCTTTGTTTAG+TGG 0.283312 7:+17048956 MsG0780036950.01.T01:CDS
TGAACTACCTCTTGAAGAAC+TGG 0.347949 7:-17048921 None:intergenic
TGTAGGAGAAAGTGAAATAA+AGG 0.373718 7:-17048604 None:intergenic
GGCTTTATATGCTGGATTGG+TGG 0.387109 7:+17048561 MsG0780036950.01.T01:CDS
CAGTTGTGGAAGTCATGAAT+TGG 0.399034 7:+17048501 None:intergenic
TTGGGCTTTATATGCTGGAT+TGG 0.428670 7:+17048558 MsG0780036950.01.T01:CDS
GCTTTATATGCTGGATTGGT+GGG 0.445941 7:+17048562 MsG0780036950.01.T01:CDS
CATGAATTGGGTTCAGCAAA+AGG 0.457099 7:+17048514 MsG0780036950.01.T01:CDS
GAAGGAGTCAAAATCTATGT+AGG 0.519982 7:-17048621 None:intergenic
GCCCAAAAGTAACATTGTAA+AGG 0.523226 7:-17048541 None:intergenic
AGTTGTGGAAGTCATGAATT+GGG 0.531926 7:+17048502 None:intergenic
ACGAAGGAAAAGACATCTGA+AGG 0.570091 7:-17048639 None:intergenic
AAACAAAGAGATGAATGAGA+AGG 0.570583 7:-17048951 None:intergenic
AACAAAGAGATGAATGAGAA+GGG 0.584648 7:-17048950 None:intergenic
CAAAAGTAACATTGTAAAGG+TGG 0.589878 7:-17048538 None:intergenic
ACATTACCCAGTTCTTCAAG+AGG 0.625963 7:+17048914 MsG0780036950.01.T01:CDS
GAACTACCTCTTGAAGAACT+GGG 0.674773 7:-17048920 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACCATGTTAGTTATTTATAT+AGG + Chr7:17048685-17048704 MsG0780036950.01.T01:intron 20.0%
!! CAAAATATCTGTAATAGTTA+AGG - Chr7:17048863-17048882 None:intergenic 20.0%
!! CCATGTTAGTTATTTATATA+GGG + Chr7:17048686-17048705 MsG0780036950.01.T01:intron 20.0%
!!! CTTAACTATTACAGATATTT+TGG + Chr7:17048861-17048880 MsG0780036950.01.T01:intron 20.0%
! CCCTATATAAATAACTAACA+TGG - Chr7:17048689-17048708 None:intergenic 25.0%
!!! ACATTTTAAGATAACTACGA+AGG - Chr7:17048658-17048677 None:intergenic 25.0%
!!! ACCTTTACAATGTTACTTTT+GGG + Chr7:17048540-17048559 MsG0780036950.01.T01:CDS 25.0%
AAACAAAGAGATGAATGAGA+AGG - Chr7:17048954-17048973 None:intergenic 30.0%
AACAAAGAGATGAATGAGAA+GGG - Chr7:17048953-17048972 None:intergenic 30.0%
CAAAAGTAACATTGTAAAGG+TGG - Chr7:17048541-17048560 None:intergenic 30.0%
TGTAGGAGAAAGTGAAATAA+AGG - Chr7:17048607-17048626 None:intergenic 30.0%
! TCATTCATCTCTTTGTTTAG+TGG + Chr7:17048956-17048975 MsG0780036950.01.T01:CDS 30.0%
!!! CACCTTTACAATGTTACTTT+TGG + Chr7:17048539-17048558 MsG0780036950.01.T01:CDS 30.0%
GAAGGAGTCAAAATCTATGT+AGG - Chr7:17048624-17048643 None:intergenic 35.0%
GCCCAAAAGTAACATTGTAA+AGG - Chr7:17048544-17048563 None:intergenic 35.0%
! TACTGATGTGTTTTGTAGTG+CGG + Chr7:17048890-17048909 MsG0780036950.01.T01:CDS 35.0%
!! TACTTTTGGGCTTTATATGC+TGG + Chr7:17048553-17048572 MsG0780036950.01.T01:CDS 35.0%
ACATTACCCAGTTCTTCAAG+AGG + Chr7:17048914-17048933 MsG0780036950.01.T01:CDS 40.0%
ACGAAGGAAAAGACATCTGA+AGG - Chr7:17048642-17048661 None:intergenic 40.0%
CATGAATTGGGTTCAGCAAA+AGG + Chr7:17048514-17048533 MsG0780036950.01.T01:CDS 40.0%
GAACTACCTCTTGAAGAACT+GGG - Chr7:17048923-17048942 None:intergenic 40.0%
TGAACTACCTCTTGAAGAAC+TGG - Chr7:17048924-17048943 None:intergenic 40.0%
! TTGGGCTTTATATGCTGGAT+TGG + Chr7:17048558-17048577 MsG0780036950.01.T01:CDS 40.0%
!! GCTTTATATGCTGGATTGGT+GGG + Chr7:17048562-17048581 MsG0780036950.01.T01:CDS 40.0%
! GGCTTTATATGCTGGATTGG+TGG + Chr7:17048561-17048580 MsG0780036950.01.T01:CDS 45.0%
Chromosome Type Strat End Strand Name
Chr7 gene 17048506 17048983 17048506 ID=MsG0780036950.01;Name=MsG0780036950.01
Chr7 mRNA 17048506 17048983 17048506 ID=MsG0780036950.01.T01;Parent=MsG0780036950.01;Name=MsG0780036950.01.T01;_AED=0.47;_eAED=0.71;_QI=0|0|0|1|0|0|2|0|92
Chr7 exon 17048506 17048675 17048506 ID=MsG0780036950.01.T01:exon:3192;Parent=MsG0780036950.01.T01
Chr7 exon 17048875 17048983 17048875 ID=MsG0780036950.01.T01:exon:3193;Parent=MsG0780036950.01.T01
Chr7 CDS 17048506 17048675 17048506 ID=MsG0780036950.01.T01:cds;Parent=MsG0780036950.01.T01
Chr7 CDS 17048875 17048983 17048875 ID=MsG0780036950.01.T01:cds;Parent=MsG0780036950.01.T01
Gene Sequence

>MsG0780036950.01.T01

GTGGAAGTCATGAATTGGGTTCAGCAAAAGGTCCACCTTTACAATGTTACTTTTGGGCTTTATATGCTGGATTGGTGGGAGCGTTGCTCATTCAGTATCCTTTATTTCACTTTCTCCTACATAGATTTTGACTCCTTCAGATGTCTTTTCCTTCGTAGTTATCTTAAAATATATTTTGGTGATTGTACTGATGTGTTTTGTAGTGCGGTACATTACCCAGTTCTTCAAGAGGTAGTTCATTTTCACCCTTCTCATTCATCTCTTTGTTTAGTGGCATAA

Protein sequence

>MsG0780036950.01.T01

VEVMNWVQQKVHLYNVTFGLYMLDWWERCSFSILYFTFSYIDFDSFRCLFLRSYLKIYFGDCTDVFCSAVHYPVLQEVVHFHPSHSSLCLVA*