AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022825.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022825.01.T01 MTR_4g094738 94.030 67 4 0 1 67 291 357 1.17e-39 133
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022825.01.T01 AT4G01220 85.075 67 10 0 1 67 293 359 1.35e-35 122
MsG0480022825.01.T01 AT1G56550 73.134 67 18 0 1 67 284 350 7.87e-34 118
MsG0480022825.01.T01 AT1G56550 73.134 67 18 0 1 67 284 350 6.11e-33 116
MsG0480022825.01.T01 AT4G01770 76.119 67 16 0 1 67 294 360 1.07e-32 115
MsG0480022825.01.T01 AT4G01750 73.134 67 18 0 1 67 300 366 4.66e-32 113

Find 23 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAAGAAGATAAAGCGATTT+CGG 0.225160 4:+77130503 MsG0480022825.01.T01:CDS
CTAAAGGCGACTCCTGTTTA+TGG 0.267123 4:-77130552 None:intergenic
AAAGAAGATAAAGCGATTTC+GGG 0.287201 4:+77130504 MsG0480022825.01.T01:CDS
CATAAACAGGAGTCGCCTTT+AGG 0.356010 4:+77130553 MsG0480022825.01.T01:CDS
GATTTCGGGACTATGGTCTA+TGG 0.390952 4:+77130518 MsG0480022825.01.T01:CDS
ATTCATAACAACTATATAGT+TGG 0.406968 4:+77130475 MsG0480022825.01.T01:CDS
CCACAGTCGGCATTCCCTAC+AGG 0.456724 4:+77130394 MsG0480022825.01.T01:CDS
ATAAAGCGATTTCGGGACTA+TGG 0.457062 4:+77130511 MsG0480022825.01.T01:CDS
GTTGGTGGATGACCATAAAC+AGG 0.476751 4:+77130540 MsG0480022825.01.T01:CDS
TTGAAATATAATCCGCCTGT+AGG 0.522041 4:-77130409 None:intergenic
GGACTATGGTCTATGGTTGG+TGG 0.522612 4:+77130525 MsG0480022825.01.T01:CDS
ATTGTACTTGCTTCCACAGT+CGG 0.528024 4:+77130381 None:intergenic
CAAGAACAAAACATGGGGTA+AGG 0.531616 4:+77130429 MsG0480022825.01.T01:CDS
TGAAATATAATCCGCCTGTA+GGG 0.540315 4:-77130408 None:intergenic
TTGAGTCATAATCCACCTAA+AGG 0.548427 4:-77130568 None:intergenic
TCGGGACTATGGTCTATGGT+TGG 0.555493 4:+77130522 MsG0480022825.01.T01:CDS
AAACAGGAGTCGCCTTTAGG+TGG 0.564100 4:+77130556 MsG0480022825.01.T01:CDS
ACATGGGGTAAGGAAACTAA+AGG 0.581819 4:+77130439 MsG0480022825.01.T01:CDS
TATATTTCAAGAACAAAACA+TGG 0.591014 4:+77130422 MsG0480022825.01.T01:CDS
ATATTTCAAGAACAAAACAT+GGG 0.618137 4:+77130423 MsG0480022825.01.T01:CDS
CCTGTAGGGAATGCCGACTG+TGG 0.638851 4:-77130394 None:intergenic
CAGTCGGCATTCCCTACAGG+CGG 0.668870 4:+77130397 MsG0480022825.01.T01:CDS
TATTTCAAGAACAAAACATG+GGG 0.687765 4:+77130424 MsG0480022825.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTTCAAGAACAAAACAT+GGG + Chr4:77130423-77130442 MsG0480022825.01.T01:CDS 20.0%
!! ATTCATAACAACTATATAGT+TGG + Chr4:77130475-77130494 MsG0480022825.01.T01:CDS 20.0%
!! TATATTTCAAGAACAAAACA+TGG + Chr4:77130422-77130441 MsG0480022825.01.T01:CDS 20.0%
! AAAAGAAGATAAAGCGATTT+CGG + Chr4:77130503-77130522 MsG0480022825.01.T01:CDS 25.0%
! TATTTCAAGAACAAAACATG+GGG + Chr4:77130424-77130443 MsG0480022825.01.T01:CDS 25.0%
AAAGAAGATAAAGCGATTTC+GGG + Chr4:77130504-77130523 MsG0480022825.01.T01:CDS 30.0%
TGAAATATAATCCGCCTGTA+GGG - Chr4:77130411-77130430 None:intergenic 35.0%
TTGAAATATAATCCGCCTGT+AGG - Chr4:77130412-77130431 None:intergenic 35.0%
ACATGGGGTAAGGAAACTAA+AGG + Chr4:77130439-77130458 MsG0480022825.01.T01:CDS 40.0%
ATAAAGCGATTTCGGGACTA+TGG + Chr4:77130511-77130530 MsG0480022825.01.T01:CDS 40.0%
CAAGAACAAAACATGGGGTA+AGG + Chr4:77130429-77130448 MsG0480022825.01.T01:CDS 40.0%
CATAAACAGGAGTCGCCTTT+AGG + Chr4:77130553-77130572 MsG0480022825.01.T01:CDS 45.0%
CTAAAGGCGACTCCTGTTTA+TGG - Chr4:77130555-77130574 None:intergenic 45.0%
GATTTCGGGACTATGGTCTA+TGG + Chr4:77130518-77130537 MsG0480022825.01.T01:CDS 45.0%
! GTTGGTGGATGACCATAAAC+AGG + Chr4:77130540-77130559 MsG0480022825.01.T01:CDS 45.0%
AAACAGGAGTCGCCTTTAGG+TGG + Chr4:77130556-77130575 MsG0480022825.01.T01:CDS 50.0%
GGACTATGGTCTATGGTTGG+TGG + Chr4:77130525-77130544 MsG0480022825.01.T01:CDS 50.0%
TCGGGACTATGGTCTATGGT+TGG + Chr4:77130522-77130541 MsG0480022825.01.T01:CDS 50.0%
CAGTCGGCATTCCCTACAGG+CGG + Chr4:77130397-77130416 MsG0480022825.01.T01:CDS 60.0%
CCACAGTCGGCATTCCCTAC+AGG + Chr4:77130394-77130413 MsG0480022825.01.T01:CDS 60.0%
! CCTGTAGGGAATGCCGACTG+TGG - Chr4:77130397-77130416 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 77130382 77130585 77130382 ID=MsG0480022825.01;Name=MsG0480022825.01
Chr4 mRNA 77130382 77130585 77130382 ID=MsG0480022825.01.T01;Parent=MsG0480022825.01;Name=MsG0480022825.01.T01;_AED=0.44;_eAED=0.45;_QI=0|-1|0|1|-1|0|1|0|67
Chr4 exon 77130382 77130585 77130382 ID=MsG0480022825.01.T01:exon:20026;Parent=MsG0480022825.01.T01
Chr4 CDS 77130382 77130585 77130382 ID=MsG0480022825.01.T01:cds;Parent=MsG0480022825.01.T01
Chr4 mRNA 77130530 77130574 77130530 ID=MsG0480022825.01.T02;Parent=MsG0480022825.01;Name=MsG0480022825.01.T02;_AED=0.49;_eAED=1.00;_QI=0|-1|0|1|-1|0|1|0|14
Chr4 exon 77130530 77130574 77130530 ID=MsG0480022825.01.T02:exon:20027;Parent=MsG0480022825.01.T02
Chr4 CDS 77130530 77130574 77130530 ID=MsG0480022825.01.T02:cds;Parent=MsG0480022825.01.T02
Gene Sequence

>MsG0480022825.01.T01

TTGTACTTGCTTCCACAGTCGGCATTCCCTACAGGCGGATTATATTTCAAGAACAAAACATGGGGTAAGGAAACTAAAGGAAAGCATGTAATTATTCATAACAACTATATAGTTGGTTTTGAAAAGAAGATAAAGCGATTTCGGGACTATGGTCTATGGTTGGTGGATGACCATAAACAGGAGTCGCCTTTAGGTGGATTATGA

>MsG0480022825.01.T02

ATGGTCTATGGTTGGTGGATGACCATAAACAGGAGTCGCCTTTAG

Protein sequence

>MsG0480022825.01.T01

LYLLPQSAFPTGGLYFKNKTWGKETKGKHVIIHNNYIVGFEKKIKRFRDYGLWLVDDHKQESPLGGL*

>MsG0480022825.01.T02

MVYGWWMTINRSRL*