AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780036567.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036567.01.T01 MTR_7g013850 51.724 87 37 2 17 102 364 446 3.49e-23 92.4
MsG0780036567.01.T01 MTR_7g013850 54.167 72 27 3 1 66 248 319 1.56e-17 76.6
MsG0780036567.01.T01 MTR_7g014670 63.265 49 18 0 54 102 177 225 1.24e-14 67.4
MsG0780036567.01.T01 MTR_7g014200 56.000 50 22 0 53 102 385 434 2.39e-14 67.4
MsG0780036567.01.T01 MTR_7g014160 54.000 50 23 0 53 102 390 439 7.57e-14 66.2
MsG0780036567.01.T01 MTR_7g014610 61.702 47 18 0 56 102 395 441 2.44e-13 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 22 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACATTGTTGTCTAAATATT+TGG 0.130040 7:+9908899 MsG0780036567.01.T01:CDS
AACAAAAGTTGACAATTTCT+TGG 0.251021 7:-9908609 None:intergenic
GGAGTATATGAGATTGATTT+TGG 0.273264 7:+9908975 MsG0780036567.01.T01:CDS
CGAACGGAATCCTTAACTTT+TGG 0.284718 7:-9908486 None:intergenic
TTCTTCAATATTGTTCCATT+TGG 0.285958 7:-9908565 None:intergenic
CGAGCTCACACGTGAAGATT+TGG 0.319044 7:+9908518 MsG0780036567.01.T01:CDS
TTTCCTTGAACCAAAAGTTA+AGG 0.387961 7:+9908476 MsG0780036567.01.T01:CDS
TCTAAATATTTGGCTATGAT+TGG 0.398839 7:+9908909 MsG0780036567.01.T01:CDS
ATTCAAGGAATAGGAGTTGC+AGG 0.408252 7:+9908939 MsG0780036567.01.T01:CDS
GGTGAAGGAATTCAAGGAAT+AGG 0.425888 7:+9908930 MsG0780036567.01.T01:CDS
ACAAAAGTGTGTTGTCCAAA+TGG 0.432381 7:+9908550 MsG0780036567.01.T01:CDS
GTTGCAGGGTCAACAAGGTT+TGG 0.454958 7:+9908954 MsG0780036567.01.T01:CDS
AGTTGATAGAGGAATACAAT+TGG 0.465205 7:+9909031 MsG0780036567.01.T01:CDS
ATGATTGGTGAAGGAATTCA+AGG 0.503765 7:+9908924 MsG0780036567.01.T01:CDS
AAATGGAACAATATTGAAGA+AGG 0.523989 7:+9908567 MsG0780036567.01.T01:CDS
TTATGGAAGGCCTGCAAAAG+TGG 0.524298 7:+9908998 MsG0780036567.01.T01:CDS
GAGACAACTTCAGTTGATAG+AGG 0.566864 7:+9909020 MsG0780036567.01.T01:CDS
TATTTGGCTATGATTGGTGA+AGG 0.577286 7:+9908915 MsG0780036567.01.T01:CDS
GAGCTCGAAGGCGACTCGAA+CGG 0.608489 7:-9908502 None:intergenic
TAGGAGTTGCAGGGTCAACA+AGG 0.619039 7:+9908949 MsG0780036567.01.T01:CDS
ATCTTCACGTGTGAGCTCGA+AGG 0.625150 7:-9908514 None:intergenic
TTCAAGGAATAGGAGTTGCA+GGG 0.658447 7:+9908940 MsG0780036567.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATCAAAAACAAAAATTTTTT+TGG + Chr7:9908689-9908708 MsG0780036567.01.T01:intron 10.0%
!! TTTATAGCAAATTAGAAAAA+TGG + Chr7:9908852-9908871 MsG0780036567.01.T01:intron 15.0%
!! TACATTGTTGTCTAAATATT+TGG + Chr7:9908899-9908918 MsG0780036567.01.T01:CDS 20.0%
! AAATGGAACAATATTGAAGA+AGG + Chr7:9908567-9908586 MsG0780036567.01.T01:CDS 25.0%
! AACAAAAGTTGACAATTTCT+TGG - Chr7:9908612-9908631 None:intergenic 25.0%
! GCAAATTAGAAAAATGGATA+AGG + Chr7:9908858-9908877 MsG0780036567.01.T01:intron 25.0%
! TCTAAATATTTGGCTATGAT+TGG + Chr7:9908909-9908928 MsG0780036567.01.T01:CDS 25.0%
! TTCTTCAATATTGTTCCATT+TGG - Chr7:9908568-9908587 None:intergenic 25.0%
!! ACAATTTCCGAAATAGTTTT+CGG - Chr7:9908756-9908775 None:intergenic 25.0%
!!! AAATTTTTTTGGTTTTGCAG+TGG + Chr7:9908700-9908719 MsG0780036567.01.T01:intron 25.0%
!!! TATGAGATTGATTTTGGTTA+TGG + Chr7:9908981-9909000 MsG0780036567.01.T01:CDS 25.0%
TTTCCTTGAACCAAAAGTTA+AGG + Chr7:9908476-9908495 MsG0780036567.01.T01:CDS 30.0%
! AATGGATAAGGTTTTCTTGA+TGG + Chr7:9908870-9908889 MsG0780036567.01.T01:intron 30.0%
! CAATTTCCGAAATAGTTTTC+GGG - Chr7:9908755-9908774 None:intergenic 30.0%
!! AGTTGATAGAGGAATACAAT+TGG + Chr7:9909031-9909050 MsG0780036567.01.T01:CDS 30.0%
!! GGAGTATATGAGATTGATTT+TGG + Chr7:9908975-9908994 MsG0780036567.01.T01:CDS 30.0%
!!! AATCCTTAACTTTTGGTTCA+AGG - Chr7:9908482-9908501 None:intergenic 30.0%
!!! AGATTGATTTTGGTTATGGA+AGG + Chr7:9908985-9909004 MsG0780036567.01.T01:CDS 30.0%
ATGATTGGTGAAGGAATTCA+AGG + Chr7:9908924-9908943 MsG0780036567.01.T01:CDS 35.0%
CCAATTCCCGAAAACTATTT+CGG + Chr7:9908746-9908765 MsG0780036567.01.T01:intron 35.0%
TATTTGGCTATGATTGGTGA+AGG + Chr7:9908915-9908934 MsG0780036567.01.T01:CDS 35.0%
! ACAAAAGTGTGTTGTCCAAA+TGG + Chr7:9908550-9908569 MsG0780036567.01.T01:CDS 35.0%
!! AAATAGTTTTCGGGAATTGG+TGG - Chr7:9908746-9908765 None:intergenic 35.0%
!! GTTTGCTTTTGCTATGCAAA+CGG - Chr7:9908654-9908673 None:intergenic 35.0%
AATCACTACTCCATCTTCCT+TGG - Chr7:9908819-9908838 None:intergenic 40.0%
ATTCAAGGAATAGGAGTTGC+AGG + Chr7:9908939-9908958 MsG0780036567.01.T01:CDS 40.0%
GAAGACTTTACCAAGGAAGA+TGG + Chr7:9908806-9908825 MsG0780036567.01.T01:intron 40.0%
GGTGAAGGAATTCAAGGAAT+AGG + Chr7:9908930-9908949 MsG0780036567.01.T01:CDS 40.0%
TCTTCCTTGGTAAAGTCTTC+TGG - Chr7:9908806-9908825 None:intergenic 40.0%
TTCAAGGAATAGGAGTTGCA+GGG + Chr7:9908940-9908959 MsG0780036567.01.T01:CDS 40.0%
! CGAACGGAATCCTTAACTTT+TGG - Chr7:9908489-9908508 None:intergenic 40.0%
!! CCGAAATAGTTTTCGGGAAT+TGG - Chr7:9908749-9908768 None:intergenic 40.0%
!! GAGACAACTTCAGTTGATAG+AGG + Chr7:9909020-9909039 MsG0780036567.01.T01:CDS 40.0%
TGAGCCAGAAGACTTTACCA+AGG + Chr7:9908799-9908818 MsG0780036567.01.T01:intron 45.0%
TTATGGAAGGCCTGCAAAAG+TGG + Chr7:9908998-9909017 MsG0780036567.01.T01:CDS 45.0%
!! GAAGTTGTCTCCACTTTTGC+AGG - Chr7:9909011-9909030 None:intergenic 45.0%
ATCTTCACGTGTGAGCTCGA+AGG - Chr7:9908517-9908536 None:intergenic 50.0%
CGAGCTCACACGTGAAGATT+TGG + Chr7:9908518-9908537 MsG0780036567.01.T01:CDS 50.0%
GTTGCAGGGTCAACAAGGTT+TGG + Chr7:9908954-9908973 MsG0780036567.01.T01:CDS 50.0%
TAGGAGTTGCAGGGTCAACA+AGG + Chr7:9908949-9908968 MsG0780036567.01.T01:CDS 50.0%
GAGCTCGAAGGCGACTCGAA+CGG - Chr7:9908505-9908524 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 9908468 9909058 9908468 ID=MsG0780036567.01;Name=MsG0780036567.01
Chr7 mRNA 9908468 9909058 9908468 ID=MsG0780036567.01.T01;Parent=MsG0780036567.01;Name=MsG0780036567.01.T01;_AED=0.40;_eAED=0.40;_QI=0|0|0|1|1|1|2|0|103
Chr7 exon 9908468 9908618 9908468 ID=MsG0780036567.01.T01:exon:1555;Parent=MsG0780036567.01.T01
Chr7 exon 9908898 9909058 9908898 ID=MsG0780036567.01.T01:exon:1556;Parent=MsG0780036567.01.T01
Chr7 CDS 9908468 9908618 9908468 ID=MsG0780036567.01.T01:cds;Parent=MsG0780036567.01.T01
Chr7 CDS 9908898 9909058 9908898 ID=MsG0780036567.01.T01:cds;Parent=MsG0780036567.01.T01
Gene Sequence

>MsG0780036567.01.T01

ATGTCAAATTTCCTTGAACCAAAAGTTAAGGATTCCGTTCGAGTCGCCTTCGAGCTCACACGTGAAGATTTGGAGAAATTAAACAAAAGTGTGTTGTCCAAATGGAACAATATTGAAGAAGGATCACAAGAACAAGAACAACCAAGAAATTATACATTGTTGTCTAAATATTTGGCTATGATTGGTGAAGGAATTCAAGGAATAGGAGTTGCAGGGTCAACAAGGTTTGGAGTATATGAGATTGATTTTGGTTATGGAAGGCCTGCAAAAGTGGAGACAACTTCAGTTGATAGAGGAATACAATTGGATTAA

Protein sequence

>MsG0780036567.01.T01

MSNFLEPKVKDSVRVAFELTREDLEKLNKSVLSKWNNIEEGSQEQEQPRNYTLLSKYLAMIGEGIQGIGVAGSTRFGVYEIDFGYGRPAKVETTSVDRGIQLD*