AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004188.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004188.01.T01 MTR_1g076720 98.630 73 1 0 6 78 295 367 2.01e-44 146
MsG0180004188.01.T01 MTR_6g060550 80.822 73 14 0 6 78 293 365 4.89e-37 127
MsG0180004188.01.T01 MTR_1g076730 82.192 73 13 0 6 78 449 521 2.15e-35 125
MsG0180004188.01.T01 MTR_3g077740 58.824 68 27 1 9 75 822 889 7.72e-18 76.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004188.01.T01 AT4G35360 82.192 73 13 0 6 78 293 365 1.16e-37 129
MsG0180004188.01.T01 AT4G35360 82.192 73 13 0 6 78 293 365 1.16e-37 129
MsG0180004188.01.T01 AT4G35360 82.192 73 13 0 6 78 196 268 2.79e-37 125
MsG0180004188.01.T01 AT2G17340 79.452 73 15 0 6 78 293 365 5.84e-36 124
MsG0180004188.01.T01 AT2G17320 78.082 73 16 0 6 78 287 359 7.95e-36 124
MsG0180004188.01.T01 AT2G17320 82.000 50 9 0 6 55 287 336 2.29e-22 88.2
MsG0180004188.01.T01 AT4G32180 52.941 68 31 1 9 75 829 896 6.24e-15 68.2

Find 17 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGATGATTACTTTCTTGC+AGG 0.279639 1:+74658434 None:intergenic
TTAAATTGAATTGAGTTTCA+TGG 0.319405 1:+74659359 MsG0180004188.01.T01:three_prime_UTR
TTCTGCCTTGTAAAAGAATC+AGG 0.323112 1:-74659281 None:intergenic
CTCTGAGAATTAACTATTTG+AGG 0.406617 1:+74659383 MsG0180004188.01.T01:three_prime_UTR
GATGAATTGTTTGATTATCA+AGG 0.417448 1:+74659475 MsG0180004188.01.T01:three_prime_UTR
CTAATCTGTATATGATCAAA+TGG 0.434621 1:-74659331 None:intergenic
CAAGATCTACATCACTGGTA+AGG 0.485155 1:-74658494 None:intergenic
ATGAATTGTTTGATTATCAA+GGG 0.497717 1:+74659476 MsG0180004188.01.T01:three_prime_UTR
TCAAGAAATTCTGCAACCTC+AGG 0.517783 1:-74659201 None:intergenic
TTGCAGAATTTCTTGAGTCA+CGG 0.536535 1:+74659208 MsG0180004188.01.T01:CDS
AAGATCTACATCACTGGTAA+GGG 0.573418 1:-74658493 None:intergenic
AATGACAAGATCTACATCAC+TGG 0.576486 1:-74658499 None:intergenic
GCAGGTTATTGATCTTACAA+AGG 0.592904 1:+74658452 MsG0180004188.01.T01:CDS
CTCAAATAGTTAATTCTCAG+AGG 0.608533 1:-74659382 None:intergenic
CTTACAGGTGAAACATCCTG+AGG 0.644122 1:+74659185 MsG0180004188.01.T01:intron
CATCTCTTATGACAGGGGCG+CGG 0.667603 1:+74659017 MsG0180004188.01.T01:intron
TGATTCTATGAAGATTGCAA+TGG 0.691158 1:+74659073 MsG0180004188.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAGTGTATTATAGTAAA+AGG + Chr1:74658767-74658786 MsG0180004188.01.T01:intron 15.0%
!! ATGAATTGTTTGATTATCAA+GGG + Chr1:74659476-74659495 MsG0180004188.01.T01:three_prime_UTR 20.0%
!! TTAAATTGAATTGAGTTTCA+TGG + Chr1:74659359-74659378 MsG0180004188.01.T01:three_prime_UTR 20.0%
!!! ATTAAAAGTACGAAGTTTAT+TGG + Chr1:74658801-74658820 MsG0180004188.01.T01:intron 20.0%
!!! TTATAAATCAATATTGACTG+TGG + Chr1:74658680-74658699 MsG0180004188.01.T01:intron 20.0%
! CAATAATTCTTGTCAGAAAT+TGG + Chr1:74658971-74658990 MsG0180004188.01.T01:intron 25.0%
! CTAATCTGTATATGATCAAA+TGG - Chr1:74659334-74659353 None:intergenic 25.0%
! GATGAATTGTTTGATTATCA+AGG + Chr1:74659475-74659494 MsG0180004188.01.T01:three_prime_UTR 25.0%
! GATTTATAAAAGTGACAGAA+AGG - Chr1:74658669-74658688 None:intergenic 25.0%
! TAACTAAATACACCTATTGA+AGG - Chr1:74658949-74658968 None:intergenic 25.0%
! TTGTCTAATCCAAATAGAAA+AGG - Chr1:74658565-74658584 None:intergenic 25.0%
!!! AATGTTGTTTTAGGAATAAG+AGG + Chr1:74659407-74659426 MsG0180004188.01.T01:three_prime_UTR 25.0%
!!! ATGTTGTTTTAGGAATAAGA+GGG + Chr1:74659408-74659427 MsG0180004188.01.T01:three_prime_UTR 25.0%
!!! ATTTGAGGTAATGTTGTTTT+AGG + Chr1:74659398-74659417 MsG0180004188.01.T01:three_prime_UTR 25.0%
!!! TAGATCTTGTCATTTTAGAA+GGG + Chr1:74658508-74658527 MsG0180004188.01.T01:CDS 25.0%
!!! TCTGTCATTATTTTTCTTAC+AGG + Chr1:74659170-74659189 MsG0180004188.01.T01:intron 25.0%
!!! TTCCTTTCTAGTTTCTTTTA+AGG - Chr1:74658849-74658868 None:intergenic 25.0%
ACACCTATTGAAGGAAAAAT+TGG - Chr1:74658940-74658959 None:intergenic 30.0%
AGCCTTAAAAGAAACTAGAA+AGG + Chr1:74658844-74658863 MsG0180004188.01.T01:intron 30.0%
CTCAAATAGTTAATTCTCAG+AGG - Chr1:74659385-74659404 None:intergenic 30.0%
TGATTCTATGAAGATTGCAA+TGG + Chr1:74659073-74659092 MsG0180004188.01.T01:CDS 30.0%
TTTGTAGAAAGGGGAAAAAA+GGG - Chr1:74658609-74658628 None:intergenic 30.0%
! CATTGTATGCCTTTTCTATT+TGG + Chr1:74658553-74658572 MsG0180004188.01.T01:intron 30.0%
! CTCTGAGAATTAACTATTTG+AGG + Chr1:74659383-74659402 MsG0180004188.01.T01:three_prime_UTR 30.0%
! TCTCCAATTTTTCCTTCAAT+AGG + Chr1:74658934-74658953 MsG0180004188.01.T01:intron 30.0%
! TTAAGCCTGATTCTTTTACA+AGG + Chr1:74659276-74659295 MsG0180004188.01.T01:three_prime_UTR 30.0%
!!! GTAGATCTTGTCATTTTAGA+AGG + Chr1:74658507-74658526 MsG0180004188.01.T01:CDS 30.0%
AAGAAACTAGAAAGGAACAG+AGG + Chr1:74658852-74658871 MsG0180004188.01.T01:intron 35.0%
AAGATCTACATCACTGGTAA+GGG - Chr1:74658496-74658515 None:intergenic 35.0%
AATGACAAGATCTACATCAC+TGG - Chr1:74658502-74658521 None:intergenic 35.0%
AGAAACTAGAAAGGAACAGA+GGG + Chr1:74658853-74658872 MsG0180004188.01.T01:intron 35.0%
CTTTGTAGAAAGGGGAAAAA+AGG - Chr1:74658610-74658629 None:intergenic 35.0%
GCAGGTTATTGATCTTACAA+AGG + Chr1:74658452-74658471 MsG0180004188.01.T01:CDS 35.0%
TTCTGCCTTGTAAAAGAATC+AGG - Chr1:74659284-74659303 None:intergenic 35.0%
TTGCAGAATTTCTTGAGTCA+CGG + Chr1:74659208-74659227 MsG0180004188.01.T01:CDS 35.0%
! ACTTCTGAGAGAGAAATATG+TGG + Chr1:74658730-74658749 MsG0180004188.01.T01:intron 35.0%
!!! TCTTGTCATTTTAGAAGGGA+TGG + Chr1:74658512-74658531 MsG0180004188.01.T01:CDS 35.0%
AACAGAGGGAGTAGTTGATT+GGG + Chr1:74658867-74658886 MsG0180004188.01.T01:intron 40.0%
AAGTGACAGAAAGGAACGAA+AGG - Chr1:74658660-74658679 None:intergenic 40.0%
ACTGACACATCTCTTATGAC+AGG + Chr1:74659010-74659029 MsG0180004188.01.T01:intron 40.0%
CAAGATCTACATCACTGGTA+AGG - Chr1:74658497-74658516 None:intergenic 40.0%
CTGACACATCTCTTATGACA+GGG + Chr1:74659011-74659030 MsG0180004188.01.T01:intron 40.0%
TCAAGAAATTCTGCAACCTC+AGG - Chr1:74659204-74659223 None:intergenic 40.0%
TGACACATCTCTTATGACAG+GGG + Chr1:74659012-74659031 MsG0180004188.01.T01:intron 40.0%
CTTACAGGTGAAACATCCTG+AGG + Chr1:74659185-74659204 MsG0180004188.01.T01:intron 45.0%
GAACAGAGGGAGTAGTTGAT+TGG + Chr1:74658866-74658885 MsG0180004188.01.T01:intron 45.0%
CCCCTTTCTACAAAGCCTAG+CGG + Chr1:74658615-74658634 MsG0180004188.01.T01:intron 50.0%
GTTGTGTGAATTTCGCCGCT+AGG - Chr1:74658633-74658652 None:intergenic 50.0%
! CCGCTAGGCTTTGTAGAAAG+GGG - Chr1:74658618-74658637 None:intergenic 50.0%
! GCCGCTAGGCTTTGTAGAAA+GGG - Chr1:74658619-74658638 None:intergenic 50.0%
CATCTCTTATGACAGGGGCG+CGG + Chr1:74659017-74659036 MsG0180004188.01.T01:intron 55.0%
! CGCCGCTAGGCTTTGTAGAA+AGG - Chr1:74658620-74658639 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 74658435 74659518 74658435 ID=MsG0180004188.01;Name=MsG0180004188.01
Chr1 mRNA 74658435 74659518 74658435 ID=MsG0180004188.01.T01;Parent=MsG0180004188.01;Name=MsG0180004188.01.T01;_AED=0.49;_eAED=0.51;_QI=0|1|0.66|1|1|1|3|252|78
Chr1 exon 74658435 74658533 74658435 ID=MsG0180004188.01.T01:exon:5635;Parent=MsG0180004188.01.T01
Chr1 exon 74659032 74659094 74659032 ID=MsG0180004188.01.T01:exon:5636;Parent=MsG0180004188.01.T01
Chr1 exon 74659192 74659518 74659192 ID=MsG0180004188.01.T01:exon:5637;Parent=MsG0180004188.01.T01
Chr1 CDS 74658435 74658533 74658435 ID=MsG0180004188.01.T01:cds;Parent=MsG0180004188.01.T01
Chr1 CDS 74659032 74659094 74659032 ID=MsG0180004188.01.T01:cds;Parent=MsG0180004188.01.T01
Chr1 CDS 74659192 74659266 74659192 ID=MsG0180004188.01.T01:cds;Parent=MsG0180004188.01.T01
Chr1 three_prime_UTR 74659267 74659518 74659267 ID=MsG0180004188.01.T01:three_prime_utr;Parent=MsG0180004188.01.T01
Gene Sequence

>MsG0180004188.01.T01

ATGATGATTACTTTCTTGCAGGTTATTGATCTTACAAAGGTGTCACAAGAGCTTGCTTCCCTTACCAGTGATGTAGATCTTGTCATTTTAGAAGGGATGGGGCGCGGAATAGAAACAAATCTCTATGCTCAATTTAAATGTGATTCTATGAAGATTGCAATGGTGAAACATCCTGAGGTTGCAGAATTTCTTGAGTCACGGATGTATGACTGTGTAATCAAATACAATGAAGTTTAG

Protein sequence

>MsG0180004188.01.T01

MMITFLQVIDLTKVSQELASLTSDVDLVILEGMGRGIETNLYAQFKCDSMKIAMVKHPEVAEFLESRMYDCVIKYNEV*