Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017661.01.T01 | XP_013462033.2 | 87.478 | 575 | 54 | 2 | 1 | 557 | 1 | 575 | 0 | 1013 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048068.01 | MsG0380017661.01 | 0.806681 | 7.308977e-50 | 3.991712e-47 |
| MsG0080048773.01 | MsG0380017661.01 | 0.831578 | 1.542266e-55 | 1.680760e-52 |
| MsG0080048780.01 | MsG0380017661.01 | 0.806380 | 8.459142e-50 | 4.584582e-47 |
| MsG0080048801.01 | MsG0380017661.01 | 0.815364 | 9.603557e-52 | 6.607577e-49 |
| MsG0080048845.01 | MsG0380017661.01 | 0.842689 | 2.218395e-58 | 3.389862e-55 |
| MsG0080048938.01 | MsG0380017661.01 | 0.811439 | 6.998677e-51 | 4.332470e-48 |
| MsG0080049040.01 | MsG0380017661.01 | 0.834869 | 2.335310e-56 | 2.807342e-53 |
| MsG0080049068.01 | MsG0380017661.01 | 0.815880 | 7.367555e-52 | 5.141291e-49 |
| MsG0080049078.01 | MsG0380017661.01 | 0.819295 | 1.251686e-52 | 9.593300e-50 |
| MsG0080049079.01 | MsG0380017661.01 | 0.801769 | 7.702465e-49 | 3.709134e-46 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017661.01.T01 | MTR_3g109650 | 87.086 | 573 | 58 | 2 | 1 | 557 | 1 | 573 | 0.0 | 1004 |
| MsG0380017661.01.T01 | MTR_3g109650 | 94.316 | 475 | 27 | 0 | 83 | 557 | 1 | 475 | 0.0 | 919 |
| MsG0380017661.01.T01 | MTR_1g006695 | 60.398 | 452 | 164 | 9 | 1 | 442 | 1 | 447 | 0.0 | 519 |
| MsG0380017661.01.T01 | MTR_8g094410 | 28.355 | 462 | 251 | 16 | 17 | 462 | 15 | 412 | 2.11e-33 | 132 |
| MsG0380017661.01.T01 | MTR_8g094410 | 28.868 | 433 | 233 | 16 | 17 | 433 | 15 | 388 | 2.87e-33 | 131 |
| MsG0380017661.01.T01 | MTR_3g096780 | 26.556 | 482 | 289 | 17 | 1 | 471 | 4 | 431 | 2.89e-31 | 126 |
| MsG0380017661.01.T01 | MTR_8g094410 | 29.517 | 393 | 208 | 17 | 76 | 462 | 34 | 363 | 1.45e-26 | 112 |
| MsG0380017661.01.T01 | MTR_8g094410 | 27.515 | 338 | 170 | 14 | 17 | 330 | 15 | 301 | 1.30e-16 | 82.4 |
| MsG0380017661.01.T01 | MTR_8g094410 | 29.589 | 365 | 191 | 15 | 76 | 433 | 34 | 339 | 2.45e-26 | 110 |
| MsG0380017661.01.T01 | MTR_8g094410 | 27.219 | 338 | 171 | 14 | 17 | 330 | 15 | 301 | 6.07e-16 | 80.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017661.01.T01 | AT5G11580 | 50.707 | 566 | 226 | 11 | 11 | 552 | 12 | 548 | 0.0 | 538 |
| MsG0380017661.01.T01 | AT5G11580 | 53.555 | 422 | 164 | 8 | 138 | 552 | 1 | 397 | 7.64e-152 | 441 |
| MsG0380017661.01.T01 | AT5G63860 | 27.842 | 431 | 243 | 15 | 16 | 433 | 16 | 391 | 2.33e-32 | 129 |
| MsG0380017661.01.T01 | AT5G63860 | 26.359 | 368 | 194 | 14 | 3 | 342 | 51 | 369 | 4.83e-20 | 93.6 |
| MsG0380017661.01.T01 | AT5G16040 | 27.361 | 413 | 237 | 18 | 35 | 436 | 6 | 366 | 6.12e-20 | 92.4 |
| MsG0380017661.01.T01 | AT3G02300 | 26.995 | 426 | 204 | 19 | 10 | 417 | 67 | 403 | 8.33e-20 | 93.2 |
| MsG0380017661.01.T01 | AT3G02300 | 26.995 | 426 | 204 | 19 | 10 | 417 | 56 | 392 | 8.57e-20 | 92.8 |
| MsG0380017661.01.T01 | AT5G12350 | 24.766 | 428 | 209 | 17 | 17 | 431 | 310 | 637 | 1.83e-18 | 90.1 |
| MsG0380017661.01.T01 | AT5G12350 | 25.305 | 328 | 202 | 10 | 20 | 335 | 365 | 661 | 5.41e-15 | 79.0 |
| MsG0380017661.01.T01 | AT4G14368 | 28.713 | 303 | 182 | 12 | 133 | 431 | 327 | 599 | 9.34e-18 | 87.8 |
Find 175 sgRNAs with CRISPR-Local
Find 296 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACGAGCATGTCGTAGCTC+TGG | 0.521902 | 3:-99263091 | MsG0380017661.01.T01:CDS |
| AAAGATCAAAGAAATTATAA+AGG | 0.357258 | 3:-99262038 | MsG0380017661.01.T01:CDS |
| AAATATGCTAGTATACTTCA+TGG | 0.467821 | 3:-99262244 | MsG0380017661.01.T01:CDS |
| AAATTGCATGAGATGAGAAG+AGG | 0.559890 | 3:+99262595 | None:intergenic |
| AACAGATGAGAAATTATCCA+CGG | 0.644744 | 3:-99262314 | MsG0380017661.01.T01:CDS |
| AACTGGGGATGTGTGGTCAT+GGG | 0.432745 | 3:-99262692 | MsG0380017661.01.T01:CDS |
| AAGAGTGTGTGATTCACCAG+CGG | 0.708326 | 3:+99265494 | None:intergenic |
| AAGAGTTGTGTTCTCGCGGG+AGG | 0.610476 | 3:-99265580 | MsG0380017661.01.T01:five_prime_UTR |
| AAGATCATTGACATCGCCGC+TGG | 0.584854 | 3:-99265510 | MsG0380017661.01.T01:CDS |
| AAGATCTACTATGCTACTCT+TGG | 0.432359 | 3:-99263041 | MsG0380017661.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAGACTCCAAGCATATTA+AGG | + | Chr3:99263763-99263782 | None:intergenic | 30.0% | |
| AACAGATGAGAAATTATCCA+CGG | - | Chr3:99265059-99265078 | MsG0380017661.01.T01:intron | 30.0% | |
| AACCAATAATAATAAGCTGG+GGG | + | Chr3:99263022-99263041 | None:intergenic | 30.0% | |
| AGAAAGATTGATCAAGTGAA+GGG | + | Chr3:99265499-99265518 | None:intergenic | 30.0% | |
| AGTTGTAAAAATACTTGACG+AGG | - | Chr3:99264414-99264433 | MsG0380017661.01.T01:intron | 30.0% | |
| CAATGGATATTGCAAAAGAA+TGG | - | Chr3:99265214-99265233 | MsG0380017661.01.T01:intron | 30.0% | |
| CCAACATATTGATCATAATG+AGG | + | Chr3:99263654-99263673 | None:intergenic | 30.0% | |
| CCTCATTATGATCAATATGT+TGG | - | Chr3:99263651-99263670 | MsG0380017661.01.T01:intron | 30.0% | |
| CTGCCAATACTTGATAAAAA+AGG | + | Chr3:99262998-99263017 | None:intergenic | 30.0% | |
| CTTTGTGGCAATTCTTTAAT+AGG | + | Chr3:99264607-99264626 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 99261769 | 99265626 | 99261769 | ID=MsG0380017661.01;Name=MsG0380017661.01 |
| Chr3 | mRNA | 99261769 | 99265626 | 99261769 | ID=MsG0380017661.01.T01;Parent=MsG0380017661.01;Name=MsG0380017661.01.T01;_AED=0.31;_eAED=0.31;_QI=49|0.66|0.5|1|1|1|4|237|557 |
| Chr3 | exon | 99261769 | 99263292 | 99261769 | ID=MsG0380017661.01.T01:exon:27288;Parent=MsG0380017661.01.T01 |
| Chr3 | exon | 99263384 | 99263546 | 99263384 | ID=MsG0380017661.01.T01:exon:27287;Parent=MsG0380017661.01.T01 |
| Chr3 | exon | 99265239 | 99265368 | 99265239 | ID=MsG0380017661.01.T01:exon:27286;Parent=MsG0380017661.01.T01 |
| Chr3 | exon | 99265484 | 99265626 | 99265484 | ID=MsG0380017661.01.T01:exon:27285;Parent=MsG0380017661.01.T01 |
| Chr3 | five_prime_UTR | 99265578 | 99265626 | 99265578 | ID=MsG0380017661.01.T01:five_prime_utr;Parent=MsG0380017661.01.T01 |
| Chr3 | CDS | 99265484 | 99265577 | 99265484 | ID=MsG0380017661.01.T01:cds;Parent=MsG0380017661.01.T01 |
| Chr3 | CDS | 99265239 | 99265368 | 99265239 | ID=MsG0380017661.01.T01:cds;Parent=MsG0380017661.01.T01 |
| Chr3 | CDS | 99263384 | 99263546 | 99263384 | ID=MsG0380017661.01.T01:cds;Parent=MsG0380017661.01.T01 |
| Chr3 | CDS | 99262006 | 99263292 | 99262006 | ID=MsG0380017661.01.T01:cds;Parent=MsG0380017661.01.T01 |
| Chr3 | three_prime_UTR | 99261769 | 99262005 | 99261769 | ID=MsG0380017661.01.T01:three_prime_utr;Parent=MsG0380017661.01.T01 |
| Gene Sequence |
>MsG0380017661.01.T01 ATGGAAGACGCCGGAAGTTATCCGAACCCCGACAAGCTATCACACAAGATCATTGACATCGCCGCTGGTGAATCACACACTCTTCTTCTTACTGGAGATGGCAGTGTATACTGTTGGGGTAGAGGAATGTTTGGACGGCTTGGGAATGGTTCTCAACAAGATGAACTTTTCCCAAAAAACTCAACTTTGGAAATCCTAATGGAACTAAACACAGTGTTAAAATATATCTGTGTCCTTGACTACTTTATGGATGGTCAACTTGGTCTTAGTGGAGAGGAGGACTCGCATGATTCCCTAGTTCCTTGCTTACTTAGCAGGTTTCATGAGTTGCAACCGCCCAATTCTTCAACAGACCCATCTGAAGCAGAAGACAAAGCATCATTAAAGATGTGTACTGTCAAAGCAGGAGGAATGATGTCTCTGGCTATTGATAACCGCAGGACCCTATGGATGTGGGGAAACATCCCACAAGAAAGCAAAGAAGGTGGGATATCTATTGTAAGCAGTTTCACACCAACACCAGTCTGGGATTTTCACGGTCGAACTGTTGTCAAGGTTTCATGTGGAAACGAGCATGTCGTAGCTCTGGTTAGTGCTATAGACTCACATGAGGATGAAGATCTACTATGCTACTCTTGGGGTTATAACAGCCATGGCCAATTAGGTTTGGGGGATAGGCAGAGCAGGCTGCGTCCAGAAGTTGTAAAAATACTTGACGAGGAGTCTCCTTGGACAATTTATGAGGTAGCATGTGGTGCCTTTCACACTGCTCTGCTTACTCACAAAAAGAAACCTGATGGCACATTAGAAAGCATGTGCTGGACCTTTGGCCTTGGTGAGAATGGCCAACTTGGACACGGAACAACGCAAAGCACTTTATTTCCTACTCCTATTAAAGAATTGCCACAAAGCATACATCTTATTTCTGTTGACTGTGGCTTGTTTCATACAAGTGTTGTGTCCTCAACTGGGGATGTGTGGTCATGGGGAATGGAGAAGGGTCTTGGCTTATGTCCTGATGCTAATCGTGCTGAAACAAATTCTGGTGGTGACGCCCTCTCTCCTCTTCTCATCTCATGCAATTTACATCAACCCATTTTTCCGGGTCCCGTTAAAGTTGTGTGTGGGGCGGCACATACTGTTGTTGTTGCTCAGGAGGGACATAAGGTGTGGTCTTGGGGTAGGGGAAGGAGTGGAGTTCTTGGGAATGGAAAGGAAATGGATAGTTACACTCCCACCATTGTTCTGTGGCCTCCAACAATTGAGGGTCTCAAAGAAGAGGAATTAAATAGCTCTGATGAGCAAGATAAGGTCGGAGAAAAAAACCCCGAAATAATAGCAGAAACAGATGAGAAATTATCCACGGTGTTGAATGAGCTGAAGCTGCTTCAAACAAAGCTATCTATAATGGAGAAATATGCTAGTATACTTCATGGTTCAATTTTTGGAAAACCTTTTAATGAGCAAGATATCCCTGTTTCAATGCAGAATTCGGGTTCAATGGATATTGCAAAAGAATGGGAGAACATGTTAGAGGCAGCAGATGATAGGAAGCTAGTTAGGATGGAAATGTTTTACCGTGACATGCTAACTGGTGTGAAAGATAAATTAATGAAGAGAAAGATCAAAGAAATTATAAAGGAGTGTCTCCAATCTTTAGAGGTAAATAAATAA |
| Protein sequence |
>MsG0380017661.01.T01 MEDAGSYPNPDKLSHKIIDIAAGESHTLLLTGDGSVYCWGRGMFGRLGNGSQQDELFPKNSTLEILMELNTVLKYICVLDYFMDGQLGLSGEEDSHDSLVPCLLSRFHELQPPNSSTDPSEAEDKASLKMCTVKAGGMMSLAIDNRRTLWMWGNIPQESKEGGISIVSSFTPTPVWDFHGRTVVKVSCGNEHVVALVSAIDSHEDEDLLCYSWGYNSHGQLGLGDRQSRLRPEVVKILDEESPWTIYEVACGAFHTALLTHKKKPDGTLESMCWTFGLGENGQLGHGTTQSTLFPTPIKELPQSIHLISVDCGLFHTSVVSSTGDVWSWGMEKGLGLCPDANRAETNSGGDALSPLLISCNLHQPIFPGPVKVVCGAAHTVVVAQEGHKVWSWGRGRSGVLGNGKEMDSYTPTIVLWPPTIEGLKEEELNSSDEQDKVGEKNPEIIAETDEKLSTVLNELKLLQTKLSIMEKYASILHGSIFGKPFNEQDIPVSMQNSGSMDIAKEWENMLEAADDRKLVRMEMFYRDMLTGVKDKLMKRKIKEIIKECLQSLEVNK* |
