AlfalfaGEDB | MsG0480018297.01

M. sativa cultivar ZhongmuNo.1 / MsG0480018297.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018297.01.T01	KEH28665.1	66.667	240	18	4	1	239	1	179	1.33E-92	281

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018297.01.T01	Q9T072	36.806	144	75	5	105	235	164	304	4.09E-14	73.9

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018297.01.T01	A0A072US12	66.667	240	18	4	1	239	1	179	6.34e-93	281

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048198.01	MsG0480018297.01	0.811812	5.804885e-51	3.628977e-48
MsG0080048773.01	MsG0480018297.01	0.844116	9.224717e-59	1.474411e-55
MsG0080048845.01	MsG0480018297.01	0.800047	1.731264e-48	7.981513e-46
MsG0080049078.01	MsG0480018297.01	0.826643	2.423722e-54	2.286299e-51
MsG0080049079.01	MsG0480018297.01	0.800238	1.583128e-48	7.334060e-46
MsG0180000120.01	MsG0480018297.01	0.804480	2.117416e-49	1.092897e-46
MsG0180000843.01	MsG0480018297.01	0.812974	3.236185e-51	2.086855e-48
MsG0180001170.01	MsG0480018297.01	0.825957	3.530094e-54	3.265709e-51
MsG0180001371.01	MsG0480018297.01	0.824815	6.579282e-54	5.891744e-51
MsG0180001929.01	MsG0480018297.01	0.800459	1.427281e-48	6.649118e-46
MsG0180003268.01	MsG0480018297.01	0.802689	4.978803e-49	2.454531e-46
MsG0180003933.01	MsG0480018297.01	0.820821	5.600047e-53	4.478500e-50
MsG0180004542.01	MsG0480018297.01	0.806659	7.387607e-50	4.032278e-47
MsG0180004987.01	MsG0480018297.01	0.801319	9.521786e-49	4.533728e-46
MsG0180005039.01	MsG0480018297.01	0.822079	2.869466e-53	2.377147e-50
MsG0180005568.01	MsG0480018297.01	0.820869	5.461638e-53	4.373769e-50
MsG0180005806.01	MsG0480018297.01	0.805266	1.450290e-49	7.638677e-47
MsG0180005807.01	MsG0480018297.01	0.809546	1.794119e-50	1.056410e-47
MsG0280010569.01	MsG0480018297.01	0.833930	4.018999e-56	4.697662e-53
MsG0280010926.01	MsG0480018297.01	0.843139	1.683816e-58	2.609459e-55
MsG0280011252.01	MsG0480018297.01	0.803854	2.858162e-49	1.451776e-46
MsG0380013177.01	MsG0480018297.01	0.829851	4.084823e-55	4.232117e-52
MsG0380013927.01	MsG0480018297.01	0.821978	3.028737e-53	2.501857e-50
MsG0380013955.01	MsG0480018297.01	0.828681	7.852399e-55	7.860291e-52
MsG0380013958.01	MsG0480018297.01	0.821344	4.245109e-53	3.444638e-50
MsG0380013970.01	MsG0480018297.01	0.837695	4.466990e-57	5.849257e-54
MsG0380014007.01	MsG0480018297.01	0.843732	1.169601e-58	1.846934e-55
MsG0380014012.01	MsG0480018297.01	0.821920	3.123421e-53	2.575967e-50
MsG0380014013.01	MsG0480018297.01	0.816239	6.126362e-52	4.317565e-49
MsG0380014022.01	MsG0480018297.01	0.833874	4.150491e-56	4.843131e-53
MsG0380014037.01	MsG0480018297.01	0.825956	3.532405e-54	3.267726e-51
MsG0380014053.01	MsG0480018297.01	0.834642	2.662961e-56	3.179513e-53
MsG0380016074.01	MsG0480018297.01	0.802448	5.584579e-49	2.736276e-46
MsG0380016137.01	MsG0480018297.01	0.803505	3.375817e-49	1.699417e-46
MsG0380016206.01	MsG0480018297.01	0.815634	8.359424e-52	5.794889e-49
MsG0380016751.01	MsG0480018297.01	0.800487	1.408643e-48	6.567105e-46
MsG0380016762.01	MsG0480018297.01	0.814771	1.300053e-51	8.801458e-49
MsG0380017087.01	MsG0480018297.01	0.800499	1.400303e-48	6.530255e-46
MsG0480018297.01	MsG0480018324.01	0.805683	1.185882e-49	6.313830e-47
MsG0480018297.01	MsG0480018844.01	0.801038	1.087160e-48	5.140043e-46
MsG0480018297.01	MsG0480018905.01	0.819237	1.290605e-52	9.875987e-50
MsG0480018297.01	MsG0480019592.01	0.804376	2.225518e-49	1.145666e-46
MsG0480018297.01	MsG0480020122.01	0.812678	3.757820e-51	2.404397e-48
MsG0480018297.01	MsG0480020408.01	0.832130	1.126719e-55	1.247863e-52
MsG0480018297.01	MsG0480021367.01	0.815604	8.492207e-52	5.881922e-49
MsG0480018297.01	MsG0480021817.01	0.811900	5.554066e-51	3.480227e-48
MsG0480018297.01	MsG0480022537.01	0.805309	1.420464e-49	7.490101e-47
MsG0480018297.01	MsG0480022804.01	0.817649	2.955250e-52	2.164616e-49
MsG0480018297.01	MsG0480023505.01	0.815296	9.940786e-52	6.826951e-49
MsG0480018297.01	MsG0480023656.01	0.800209	1.604223e-48	7.426386e-46
MsG0480018297.01	MsG0580024782.01	-0.808750	2.656825e-50	1.531490e-47
MsG0480018297.01	MsG0580025054.01	0.810315	1.225215e-50	7.361402e-48
MsG0480018297.01	MsG0580026430.01	0.805085	1.582556e-49	8.296990e-47
MsG0480018297.01	MsG0580028925.01	0.810830	9.481991e-51	5.775916e-48
MsG0480018297.01	MsG0580029291.01	-0.804219	2.399118e-49	1.230014e-46
MsG0480018297.01	MsG0580029529.01	0.836332	9.957858e-57	1.251051e-53
MsG0480018297.01	MsG0580029918.01	0.811832	5.747410e-51	3.594868e-48
MsG0480018297.01	MsG0680030680.01	0.802924	4.452772e-49	2.208372e-46
MsG0480018297.01	MsG0680030776.01	0.809899	1.505961e-50	8.950507e-48
MsG0480018297.01	MsG0680034724.01	0.806001	1.016938e-49	5.458684e-47
MsG0480018297.01	MsG0680035308.01	0.805904	1.065462e-49	5.704869e-47
MsG0480018297.01	MsG0780037705.01	0.808205	3.471928e-50	1.973141e-47
MsG0480018297.01	MsG0780038571.01	0.835834	1.331783e-56	1.648008e-53
MsG0480018297.01	MsG0780038653.01	0.803438	3.485277e-49	1.751439e-46
MsG0480018297.01	MsG0780039186.01	0.803015	4.264754e-49	2.119878e-46
MsG0480018297.01	MsG0780039975.01	0.833814	4.296829e-56	5.004666e-53
MsG0480018297.01	MsG0780040080.01	0.809260	2.066093e-50	1.207323e-47
MsG0480018297.01	MsG0780040700.01	0.824809	6.600977e-54	5.910160e-51
MsG0480018297.01	MsG0780040825.01	-0.806594	7.627078e-50	4.156107e-47
MsG0480018297.01	MsG0780040935.01	0.800460	1.426228e-48	6.644488e-46
MsG0480018297.01	MsG0780041004.01	0.814066	1.861568e-51	1.236582e-48
MsG0480018297.01	MsG0880042555.01	0.800934	1.141742e-48	5.383762e-46
MsG0480018297.01	MsG0880042876.01	0.822528	2.257372e-53	1.894365e-50
MsG0480018297.01	MsG0880043250.01	0.805259	1.455331e-49	7.663707e-47
MsG0480018297.01	MsG0880044538.01	0.821329	4.279068e-53	3.470802e-50
MsG0480018297.01	MsG0880044558.01	0.802131	6.490006e-49	3.154323e-46
MsG0480018297.01	MsG0880044884.01	0.850838	1.310760e-60	2.601574e-57
MsG0480018297.01	MsG0880045283.01	0.817603	3.027009e-52	2.214359e-49
MsG0480018297.01	MsG0880045668.01	0.800554	1.365162e-48	6.375164e-46
MsG0480018297.01	MsG0880046255.01	0.819288	1.256265e-52	9.626614e-50
MsG0480018297.01	MsG0880046456.01	0.802631	5.119377e-49	2.520081e-46
MsG0480018297.01	MsG0880046735.01	0.800834	1.196909e-48	5.629403e-46
MsG0480018297.01	MsG0880047156.01	0.814784	1.291292e-51	8.745675e-49
MsG0480018297.01	MsG0880047460.01	-0.806172	9.360498e-50	5.046399e-47
MsG0480018297.01	MsG0880047656.01	0.801792	7.618999e-49	3.671211e-46
MsG0280006848.01	MsG0480018297.01	0.814453	1.528849e-51	1.026273e-48
MsG0280007041.01	MsG0480018297.01	0.809987	1.441675e-50	8.588645e-48
MsG0280007628.01	MsG0480018297.01	0.821787	3.353385e-53	2.755212e-50
MsG0280007976.01	MsG0480018297.01	0.812992	3.207005e-51	2.069090e-48
MsG0280008233.01	MsG0480018297.01	0.804130	2.504103e-49	1.280926e-46
MsG0280008709.01	MsG0480018297.01	0.802380	5.766076e-49	2.820533e-46
MsG0280008966.01	MsG0480018297.01	0.803784	2.955326e-49	1.498389e-46
MsG0280009635.01	MsG0480018297.01	0.808671	2.761946e-50	1.588809e-47
MsG0280009869.01	MsG0480018297.01	0.828529	8.548530e-55	8.519400e-52
MsG0280009953.01	MsG0480018297.01	0.814205	1.734695e-51	1.156583e-48
MsG0280010198.01	MsG0480018297.01	0.818320	2.084559e-52	1.555288e-49
MsG0280010417.01	MsG0480018297.01	-0.801962	7.030005e-49	3.401993e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018297.01.T01	MTR_4g009420	66.667	240	18	4	1	239	1	179	1.61e-96	281
MsG0480018297.01.T01	MTR_4g009420	65.823	237	19	4	1	236	1	176	1.11e-93	273
MsG0480018297.01.T01	MTR_7g451650	63.750	240	25	5	1	239	1	179	2.32e-87	257
MsG0480018297.01.T01	MTR_7g451650	62.869	237	26	5	1	236	1	176	2.34e-84	250
MsG0480018297.01.T01	MTR_4g009510	55.738	244	52	5	1	242	1	190	4.93e-74	224
MsG0480018297.01.T01	MTR_7g050350	80.000	115	21	2	123	236	1	114	2.65e-53	169
MsG0480018297.01.T01	MTR_7g451650	80.000	115	21	2	123	236	1	114	2.65e-53	169
MsG0480018297.01.T01	MTR_2g104550	45.736	129	55	3	121	236	165	291	3.67e-21	90.5
MsG0480018297.01.T01	MTR_4g009110	33.143	175	100	4	77	236	157	329	3.82e-20	88.2
MsG0480018297.01.T01	MTR_4g009540	36.667	150	76	3	105	237	164	311	1.27e-19	86.3
MsG0480018297.01.T01	MTR_4g009540	36.242	149	76	3	105	236	164	310	1.25e-18	84.0
MsG0480018297.01.T01	MTR_5g014520	38.636	132	71	1	121	242	184	315	4.73e-18	82.0
MsG0480018297.01.T01	MTR_7g060475	44.954	109	48	4	83	191	55	151	1.06e-17	78.6
MsG0480018297.01.T01	MTR_3g116670	72.727	44	12	0	82	125	72	115	4.47e-17	75.5
MsG0480018297.01.T01	MTR_2g104560	39.200	125	66	3	121	236	81	204	1.68e-16	76.3
MsG0480018297.01.T01	MTR_2g104500	39.496	119	65	2	124	236	134	251	2.45e-16	76.6
MsG0480018297.01.T01	MTR_5g014600	38.129	139	63	4	121	236	176	314	2.64e-16	77.4
MsG0480018297.01.T01	MTR_2g104530	39.837	123	68	1	120	236	131	253	4.16e-16	76.3
MsG0480018297.01.T01	MTR_2g436990	49.383	81	39	1	45	125	208	286	5.40e-16	76.6
MsG0480018297.01.T01	MTR_5g014560	36.364	132	74	2	121	242	158	289	1.72e-15	74.7
MsG0480018297.01.T01	MTR_0250s0040	34.091	132	65	4	121	236	115	240	3.15e-15	73.6
MsG0480018297.01.T01	MTR_7g070905	67.442	43	14	0	83	125	12	54	7.25e-15	68.2
MsG0480018297.01.T01	MTR_2g104490	38.168	131	67	2	121	240	164	291	8.44e-15	72.8
MsG0480018297.01.T01	MTR_2g010450	28.279	244	147	7	7	238	105	332	1.21e-14	72.8
MsG0480018297.01.T01	MTR_5g023580	73.171	41	11	0	85	125	287	327	1.24e-14	72.8
MsG0480018297.01.T01	MTR_8g009020	33.766	154	74	4	105	236	155	302	3.63e-14	71.2
MsG0480018297.01.T01	MTR_7g091670	70.455	44	13	0	82	125	29	72	3.67e-14	67.8
MsG0480018297.01.T01	MTR_8g009010	35.897	117	48	2	121	236	161	251	3.84e-14	70.9
MsG0480018297.01.T01	MTR_5g014550	37.705	122	66	2	124	235	54	175	4.18e-14	69.3
MsG0480018297.01.T01	MTR_2g104490	37.795	127	65	2	121	236	164	287	4.65e-14	70.9
MsG0480018297.01.T01	MTR_4g092700	31.724	145	88	4	105	239	170	313	1.16e-13	69.7
MsG0480018297.01.T01	MTR_3g449780	69.767	43	13	0	83	125	14	56	1.70e-13	64.7
MsG0480018297.01.T01	MTR_4g067010	34.109	129	66	2	121	236	174	296	2.92e-13	68.6
MsG0480018297.01.T01	MTR_0048s0210	57.407	54	21	1	83	136	15	66	2.98e-13	65.5
MsG0480018297.01.T01	MTR_0048s0210	64.103	39	14	0	83	121	93	131	5.65e-11	58.9
MsG0480018297.01.T01	MTR_8g097110	59.184	49	20	0	77	125	13	61	3.35e-13	63.5
MsG0480018297.01.T01	MTR_1g060110	66.667	42	14	0	84	125	10	51	3.38e-13	63.9
MsG0480018297.01.T01	MTR_4g066460	34.884	129	65	3	121	236	182	304	4.72e-13	67.8
MsG0480018297.01.T01	MTR_4g129250	68.293	41	13	0	83	123	40	80	8.10e-13	62.8
MsG0480018297.01.T01	MTR_4g066380	52.542	59	28	0	178	236	243	301	1.07e-12	67.0
MsG0480018297.01.T01	MTR_3g014560	76.471	34	8	0	92	125	3	36	1.10e-12	63.9
MsG0480018297.01.T01	MTR_5g014640	34.677	124	74	2	121	237	181	304	1.48e-12	66.6
MsG0480018297.01.T01	MTR_0011s0260	31.746	126	76	1	121	236	210	335	1.66e-12	66.6
MsG0480018297.01.T01	MTR_6g074890	49.180	61	31	0	82	142	62	122	2.01e-12	63.5
MsG0480018297.01.T01	MTR_6g047570	34.091	132	65	4	121	236	186	311	2.05e-12	66.2
MsG0480018297.01.T01	MTR_0011s0210	30.952	126	77	1	121	236	210	335	2.39e-12	66.2
MsG0480018297.01.T01	MTR_7g093150	65.000	40	14	0	83	122	44	83	2.57e-11	58.9
MsG0480018297.01.T01	MTR_7g101000	71.795	39	11	0	83	121	34	72	4.18e-11	58.5
MsG0480018297.01.T01	MTR_8g040390	64.583	48	16	1	83	130	94	140	5.57e-11	59.3
MsG0480018297.01.T01	MTR_6g016695	60.000	40	16	0	83	122	40	79	5.89e-11	57.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018297.01.T01	AT4G37850	36.806	144	75	5	105	235	164	304	4.17e-15	73.9
MsG0480018297.01.T01	AT4G37850	36.806	144	75	5	105	235	164	304	4.17e-15	73.9
MsG0480018297.01.T01	AT2G22760	34.559	136	76	3	121	243	146	281	8.03e-14	69.7
MsG0480018297.01.T01	AT2G22760	33.333	129	73	3	121	236	146	274	1.37e-12	66.2
MsG0480018297.01.T01	AT2G22770	32.624	141	70	2	121	236	159	299	3.89e-11	62.4

Find 62 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAGTAGAGTCTTGAATTT+TGG	0.151614	4:-3034928	MsG0480018297.01.T01:CDS
AAATGCTATTTAATGAATTA+TGG	0.190163	4:-3034745	MsG0480018297.01.T01:three_prime_UTR
ATAATAATATATATTCTATT+TGG	0.210339	4:-3034815	MsG0480018297.01.T01:three_prime_UTR
TACATTACATATATAGTTCT+TGG	0.239735	4:-3038421	MsG0480018297.01.T01:five_prime_UTR
TTGGGTTGTTATATAATATA+GGG	0.248624	4:+3038486	None:intergenic
AGTTCTTGGAACAAAATTAA+TGG	0.286364	4:-3038407	MsG0480018297.01.T01:exon
TTGGCCTTCTAATTCTGATT+TGG	0.292992	4:-3038368	MsG0480018297.01.T01:intron
TTTACCAAATCAGAATTAGA+AGG	0.359501	4:+3038364	None:intergenic
GTTGGGTTGTTATATAATAT+AGG	0.359629	4:+3038485	None:intergenic
TGGAGGAGTCGGGAGAAAAT+TGG	0.405975	4:-3038387	MsG0480018297.01.T01:CDS
AACACTCTTGCTTTGACCTC+TGG	0.412988	4:+3035048	None:intergenic
GGTTGTGAAGGTGAGTAATA+TGG	0.419505	4:+3038569	None:intergenic
ACGAACCTTCTTTAAGCCTA+TGG	0.424699	4:+3038066	None:intergenic
TGGGTTGTTATATAATATAG+GGG	0.430477	4:+3038487	None:intergenic
GGAGATCACAGAGATGTTGC+AGG	0.441683	4:-3038101	MsG0480018297.01.T01:CDS
GTCCAATGTGAGGAACAAAA+TGG	0.442895	4:-3035003	MsG0480018297.01.T01:CDS
AGATTCTCCATCTTTAAGAA+TGG	0.468345	4:+3038213	None:intergenic
ACTTCTACTATAACATCTAC+TGG	0.470607	4:+3034716	None:intergenic
GGATCATTATTGTGAAGATC+AGG	0.473237	4:-3038164	MsG0480018297.01.T01:CDS
GAAAGTACAGTGTGGATAAG+AGG	0.476342	4:+3038459	None:intergenic
GTGTGGATAAGAGGTGGGTT+GGG	0.484793	4:+3038468	None:intergenic
CAATGAGTGAGGGGTATCCT+TGG	0.492087	4:-3034650	None:intergenic
AGTGTGGATAAGAGGTGGGT+TGG	0.494204	4:+3038467	None:intergenic
CTAACACAATCCCACAAAAC+CGG	0.498603	4:+3036693	None:intergenic
AAACAAGAGAATGGTGGTTG+AGG	0.500954	4:+3038548	None:intergenic
TACCCCTCACTCATTGATCA+TGG	0.501812	4:+3034657	None:intergenic
GATGAAGCCATTCTTAAAGA+TGG	0.508496	4:-3038220	MsG0480018297.01.T01:CDS
GTCATATATCTGATAGAAGA+TGG	0.509177	4:-3036760	MsG0480018297.01.T01:CDS
AACAAAATTAATGGAGGAGT+CGG	0.509366	4:-3038398	MsG0480018297.01.T01:exon
TCCAACCACGATTTCTAAGA+TGG	0.518746	4:-3036669	MsG0480018297.01.T01:intron
AGTTCAAATTACTACAATTG+TGG	0.519204	4:-3035093	MsG0480018297.01.T01:CDS
CAGGAACTTTCAGACGCCAT+AGG	0.519378	4:-3038082	MsG0480018297.01.T01:CDS
AGACGCCATAGGCTTAAAGA+AGG	0.520059	4:-3038071	MsG0480018297.01.T01:CDS
GATGAATTGAGTCGCTCCAC+AGG	0.530531	4:-3038602	MsG0480018297.01.T01:five_prime_UTR
ATTGACTTGTATATGTACCT+TGG	0.538882	4:+3034876	None:intergenic
AGAGAGCTAGAACAAGAAGC+TGG	0.540149	4:-3035147	MsG0480018297.01.T01:CDS
AGTACAGTGTGGATAAGAGG+TGG	0.567387	4:+3038462	None:intergenic
ACAAAATTAATGGAGGAGTC+GGG	0.571828	4:-3038397	MsG0480018297.01.T01:exon
CATTATTGTGAAGATCAGGT+TGG	0.572263	4:-3038160	MsG0480018297.01.T01:CDS
GATTCAACATTTGTAACGAA+AGG	0.572379	4:-3035125	MsG0480018297.01.T01:CDS
TGACATCACCATTGTTGCCA+AGG	0.577300	4:-3034893	MsG0480018297.01.T01:CDS
ACCATCTTAGAAATCGTGGT+TGG	0.578198	4:+3036668	None:intergenic
GTACAGTGTGGATAAGAGGT+GGG	0.582768	4:+3038463	None:intergenic
TACAAAGGAAAACAATGAGA+AGG	0.583439	4:-3038122	MsG0480018297.01.T01:CDS
GGGTTGTTATATAATATAGG+GGG	0.595894	4:+3038488	None:intergenic
TATCCATGATCAATGAGTGA+GGG	0.597713	4:-3034660	MsG0480018297.01.T01:three_prime_UTR
TGGCAACAATGGTGATGTCA+AGG	0.603091	4:+3034896	None:intergenic
GGTTGTTATATAATATAGGG+GGG	0.613478	4:+3038489	None:intergenic
AATGGTGGTTGAGGTTGTGA+AGG	0.616577	4:+3038557	None:intergenic
TCTTGGAACAAAATTAATGG+AGG	0.624977	4:-3038404	MsG0480018297.01.T01:exon
TGATACCATCTTAGAAATCG+TGG	0.627201	4:+3036664	None:intergenic
TATATGTACCTTGGCAACAA+TGG	0.630484	4:+3034885	None:intergenic
ATCCATGATCAATGAGTGAG+GGG	0.635555	4:-3034659	MsG0480018297.01.T01:three_prime_UTR
ATATCCATGATCAATGAGTG+AGG	0.651570	4:-3034661	MsG0480018297.01.T01:three_prime_UTR
GGCAACAATGGTGATGTCAA+GGG	0.668997	4:+3034897	None:intergenic
ATATGGTGAGTAGAGTCCTG+TGG	0.682152	4:+3038586	None:intergenic
TGTATGTGATGATGATGATG+AGG	0.683020	4:-3038185	MsG0480018297.01.T01:CDS
AGATCCCACAGATAATACAA+AGG	0.692675	4:-3038137	MsG0480018297.01.T01:CDS
AACAATGGTGATGTCAAGGG+TGG	0.697013	4:+3034900	None:intergenic
TGATATAATTACTCTTCCAG+AGG	0.698719	4:-3035064	MsG0480018297.01.T01:CDS
ACTCGTCATAGTCCAATGTG+AGG	0.715682	4:-3035013	MsG0480018297.01.T01:CDS
TGAAGAATGAAAGTACAGTG+TGG	0.725809	4:+3038451	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTTTTATTATAATTTAA+AGG	-	Chr4:3037075-3037094	MsG0480018297.01.T01:intron	0.0%
!!	AATCATATGTATTATATAAT+AGG	+	Chr4:3034953-3034972	None:intergenic	10.0%
!!	ATATTTATACTTTAAATGAA+TGG	-	Chr4:3035259-3035278	MsG0480018297.01.T01:intron	10.0%
!!!	AATATTTTTTAGTATAAAGA+AGG	+	Chr4:3035472-3035491	None:intergenic	10.0%
!!!	ATTTTTGTAAATGTTATTAA+TGG	+	Chr4:3035850-3035869	None:intergenic	10.0%
!!!	CTTCATATAATATATTTTTA+AGG	-	Chr4:3038424-3038443	MsG0480018297.01.T01:five_prime_UTR	10.0%
!!	ATATGCACTTATAAATAATA+GGG	-	Chr4:3037999-3038018	MsG0480018297.01.T01:intron	15.0%
!!	TATATGCACTTATAAATAAT+AGG	-	Chr4:3037998-3038017	MsG0480018297.01.T01:intron	15.0%
!!	TTATTTAGATTGAAAGAAAA+GGG	-	Chr4:3037717-3037736	MsG0480018297.01.T01:intron	15.0%
!!	TTTAACTATCTACTAATTAA+TGG	+	Chr4:3036245-3036264	None:intergenic	15.0%
!!!	AAATGCTATTTAATGAATTA+TGG	-	Chr4:3038527-3038546	MsG0480018297.01.T01:five_prime_UTR	15.0%
!!!	AATATAAGGAACTTTATTTA+TGG	+	Chr4:3035973-3035992	None:intergenic	15.0%
!!!	TTTTTGTACCAATTAATTTT+GGG	+	Chr4:3037128-3037147	None:intergenic	15.0%
!!	AAGAAAAAAAAACAAGAGAA+TGG	+	Chr4:3034736-3034755	None:intergenic	20.0%
!!	ATATCCATGGATAAAATAAA+CGG	+	Chr4:3036272-3036291	None:intergenic	20.0%
!!	CTTATTTAGATTGAAAGAAA+AGG	-	Chr4:3037716-3037735	MsG0480018297.01.T01:intron	20.0%
!!	CTTCAACAAAAAAAAAAAAC+TGG	+	Chr4:3035309-3035328	None:intergenic	20.0%
!!	TAAATTCCTCGATTAAATAA+CGG	-	Chr4:3036173-3036192	MsG0480018297.01.T01:intron	20.0%
!!	TACATTACATATATAGTTCT+TGG	-	Chr4:3034851-3034870	MsG0480018297.01.T01:exon	20.0%
!!	TAGATTTCATATTTATCCAA+CGG	+	Chr4:3035747-3035766	None:intergenic	20.0%
!!	TATTTAGATTGAAAGAAAAG+GGG	-	Chr4:3037718-3037737	MsG0480018297.01.T01:intron	20.0%
!!	TTTCTTTCAATCTAAATAAG+TGG	+	Chr4:3037716-3037735	None:intergenic	20.0%
!!!	ACAATTTTCATATTGTACAA+CGG	-	Chr4:3037908-3037927	MsG0480018297.01.T01:intron	20.0%
!!!	ACTTTTTTTTTAGGAAATCT+TGG	+	Chr4:3038588-3038607	None:intergenic	20.0%
!!!	CAGTTTTTTTTTTTTGTTGA+AGG	-	Chr4:3035307-3035326	MsG0480018297.01.T01:intron	20.0%
!!!	CTTTTTGTACCAATTAATTT+TGG	+	Chr4:3037129-3037148	None:intergenic	20.0%
!!!	GGATATATGACTTTTTTTTT+AGG	+	Chr4:3038597-3038616	None:intergenic	20.0%
!!!	GGTTTTATCATATTATTTGT+AGG	+	Chr4:3037473-3037492	None:intergenic	20.0%
!!!	TGTTTTTTGCATTTATTCAT+AGG	-	Chr4:3035021-3035040	MsG0480018297.01.T01:CDS	20.0%
!!!	TTGGGTTGTTATATAATATA+GGG	+	Chr4:3034789-3034808	None:intergenic	20.0%
!!!	TTTTGCTTAAGAAAGTATTA+AGG	-	Chr4:3036141-3036160	MsG0480018297.01.T01:intron	20.0%
!!!	TTTTTTAGTATAAAGAAGGT+TGG	+	Chr4:3035468-3035487	None:intergenic	20.0%
!	AAAAAAAAACAAGAGAATGG+TGG	+	Chr4:3034733-3034752	None:intergenic	25.0%
!	AAATTCCTCGATTAAATAAC+GGG	-	Chr4:3036174-3036193	MsG0480018297.01.T01:intron	25.0%
!	ACAAATTCAACCAATGATTT+AGG	+	Chr4:3037540-3037559	None:intergenic	25.0%
!	AGTTCAAATTACTACAATTG+TGG	-	Chr4:3038179-3038198	MsG0480018297.01.T01:CDS	25.0%
!	AGTTCTTGGAACAAAATTAA+TGG	-	Chr4:3034865-3034884	MsG0480018297.01.T01:CDS	25.0%
!	CAATGAAAAGAAATCATTGT+CGG	-	Chr4:3037186-3037205	MsG0480018297.01.T01:intron	25.0%
!	GAAATCACAAAAAACACAAA+AGG	-	Chr4:3037668-3037687	MsG0480018297.01.T01:intron	25.0%
!	TAAAATCTTACTTAAGAGTG+TGG	-	Chr4:3036451-3036470	MsG0480018297.01.T01:intron	25.0%
!	TGGGTTGTTATATAATATAG+GGG	+	Chr4:3034788-3034807	None:intergenic	25.0%
!	TTATTATACGCTCCTTTAAA+AGG	+	Chr4:3036099-3036118	None:intergenic	25.0%
!	TTTACCAAATCAGAATTAGA+AGG	+	Chr4:3034911-3034930	None:intergenic	25.0%
!	TTTCACAAAACCAATTTATG+AGG	+	Chr4:3037004-3037023	None:intergenic	25.0%
!!	ATTTTATCCACGAATATTCA+CGG	-	Chr4:3036324-3036343	MsG0480018297.01.T01:intron	25.0%
!!	GTTGGGTTGTTATATAATAT+AGG	+	Chr4:3034790-3034809	None:intergenic	25.0%
!!	TTTTCCTTTGTATTATCTGT+GGG	+	Chr4:3035142-3035161	None:intergenic	25.0%
!!!	ACCAATTAATTTTGGGAAAA+GGG	+	Chr4:3037121-3037140	None:intergenic	25.0%
!!!	ATATCCGTTTATTTTATCCA+TGG	-	Chr4:3036265-3036284	MsG0480018297.01.T01:intron	25.0%
!!!	TACCAATTAATTTTGGGAAA+AGG	+	Chr4:3037122-3037141	None:intergenic	25.0%
!!!	TTTTTAGTCGATGATCTTTA+TGG	+	Chr4:3035945-3035964	None:intergenic	25.0%
	AAAAGTCGATACCTTCAATT+AGG	+	Chr4:3036370-3036389	None:intergenic	30.0%
	AAAGCTAGCTCAAATGATAA+AGG	+	Chr4:3036773-3036792	None:intergenic	30.0%
	AAAGTCGATACCTTCAATTA+GGG	+	Chr4:3036369-3036388	None:intergenic	30.0%
	AACAAAATTAATGGAGGAGT+CGG	-	Chr4:3034874-3034893	MsG0480018297.01.T01:CDS	30.0%
	AACATCGCTAAAAACGTAAA+AGG	-	Chr4:3037392-3037411	MsG0480018297.01.T01:intron	30.0%
	ACTTCTACTATAACATCTAC+TGG	+	Chr4:3038559-3038578	None:intergenic	30.0%
	AGATCCAAACCTATTTAAGA+TGG	-	Chr4:3036799-3036818	MsG0480018297.01.T01:intron	30.0%
	ATATTGTACAACGGATAAGT+TGG	-	Chr4:3037917-3037936	MsG0480018297.01.T01:intron	30.0%
	ATCATCTTGAGTTTGTCATT+AGG	+	Chr4:3034653-3034672	None:intergenic	30.0%
	GACAATGTGTTTATAAGTGA+GGG	-	Chr4:3036538-3036557	MsG0480018297.01.T01:intron	30.0%
	GATACCATCTTAAATAGGTT+TGG	+	Chr4:3036806-3036825	None:intergenic	30.0%
	GATTCAACATTTGTAACGAA+AGG	-	Chr4:3038147-3038166	MsG0480018297.01.T01:CDS	30.0%
	GGGTTGTTATATAATATAGG+GGG	+	Chr4:3034787-3034806	None:intergenic	30.0%
	GGTTGTTATATAATATAGGG+GGG	+	Chr4:3034786-3034805	None:intergenic	30.0%
	GTCATATATCTGATAGAAGA+TGG	-	Chr4:3036512-3036531	MsG0480018297.01.T01:intron	30.0%
	TAAATGGATATCCATTGATG+TGG	-	Chr4:3035698-3035717	MsG0480018297.01.T01:intron	30.0%
	TACAAAGGAAAACAATGAGA+AGG	-	Chr4:3035150-3035169	MsG0480018297.01.T01:CDS	30.0%
	TCCAAGTTAATAGCATACAA+CGG	-	Chr4:3037440-3037459	MsG0480018297.01.T01:intron	30.0%
	TCTCTATATAAATGTGTAGC+TGG	-	Chr4:3038043-3038062	MsG0480018297.01.T01:intron	30.0%
	TCTTGGAACAAAATTAATGG+AGG	-	Chr4:3034868-3034887	MsG0480018297.01.T01:CDS	30.0%
	TGACAATGTGTTTATAAGTG+AGG	-	Chr4:3036537-3036556	MsG0480018297.01.T01:intron	30.0%
	TGATATAATTACTCTTCCAG+AGG	-	Chr4:3038208-3038227	MsG0480018297.01.T01:CDS	30.0%
!	AACAGTAGAGTCTTGAATTT+TGG	-	Chr4:3038344-3038363	MsG0480018297.01.T01:intron	30.0%
!	AACATAGCTTTTTGCTTCAA+CGG	-	Chr4:3036055-3036074	MsG0480018297.01.T01:intron	30.0%
!	AGATTCTCCATCTTTAAGAA+TGG	+	Chr4:3035062-3035081	None:intergenic	30.0%
!	ATTGACTTTCTTAGAAATCG+TGG	+	Chr4:3037042-3037061	None:intergenic	30.0%
!	CGACAATGATTTCTTTTCAT+TGG	+	Chr4:3037188-3037207	None:intergenic	30.0%
!	CTGAAAGATGAAGATTTTCA+AGG	+	Chr4:3038319-3038338	None:intergenic	30.0%
!	GTTTTCCTTTGTATTATCTG+TGG	+	Chr4:3035143-3035162	None:intergenic	30.0%
!	TAAGAGTGTGGTTTAAACAA+AGG	-	Chr4:3036463-3036482	MsG0480018297.01.T01:intron	30.0%
!	TCCCTTTTCCCAAAATTAAT+TGG	-	Chr4:3037117-3037136	MsG0480018297.01.T01:intron	30.0%
!	TTTTTGTCATCCCTAATTGA+AGG	-	Chr4:3036356-3036375	MsG0480018297.01.T01:intron	30.0%
!!	AAGATGAAGATTTTCAAGGT+TGG	+	Chr4:3038315-3038334	None:intergenic	30.0%
!!	AGATGTAGATGACTGATTTT+AGG	-	Chr4:3037748-3037767	MsG0480018297.01.T01:intron	30.0%
!!	ATTGACTTGTATATGTACCT+TGG	+	Chr4:3038399-3038418	None:intergenic	30.0%
!!!	TGGTTTTGTGAAATTGTGTT+AGG	-	Chr4:3037011-3037030	MsG0480018297.01.T01:intron	30.0%
!!!	TTATCATTTGAGCTAGCTTT+TGG	-	Chr4:3036773-3036792	MsG0480018297.01.T01:CDS	30.0%
	AATAGGTACGTGATTGCAAT+TGG	+	Chr4:3034936-3034955	None:intergenic	35.0%
	AATCACACTATCCACATCAA+TGG	+	Chr4:3035712-3035731	None:intergenic	35.0%
	ACAAAATTAATGGAGGAGTC+GGG	-	Chr4:3034875-3034894	MsG0480018297.01.T01:CDS	35.0%
	ACCGTTGTATGCTATTAACT+TGG	+	Chr4:3037444-3037463	None:intergenic	35.0%
	ACTGATGTCGATCTTTCAAT+AGG	-	Chr4:3037565-3037584	MsG0480018297.01.T01:intron	35.0%
	AGATCCCACAGATAATACAA+AGG	-	Chr4:3035135-3035154	MsG0480018297.01.T01:CDS	35.0%
	AGTGACTTAAGATTGAAGGT+AGG	-	Chr4:3035671-3035690	MsG0480018297.01.T01:intron	35.0%
	ATATCCATGATCAATGAGTG+AGG	-	Chr4:3038611-3038630	MsG0480018297.01.T01:five_prime_UTR	35.0%
	ATTGAAGGTAGGGATGTAAA+TGG	-	Chr4:3035682-3035701	MsG0480018297.01.T01:intron	35.0%
	CAAAACCAATTTATGAGGTG+AGG	+	Chr4:3036999-3037018	None:intergenic	35.0%
	CATTATTGTGAAGATCAGGT+TGG	-	Chr4:3035112-3035131	MsG0480018297.01.T01:CDS	35.0%
	GATGAAGCCATTCTTAAAGA+TGG	-	Chr4:3035052-3035071	MsG0480018297.01.T01:CDS	35.0%
	GCAAATAATGCAAATGCCTT+TGG	+	Chr4:3037249-3037268	None:intergenic	35.0%
	GCTCTGATACCATCTTAAAT+AGG	+	Chr4:3036811-3036830	None:intergenic	35.0%
	GGATCATTATTGTGAAGATC+AGG	-	Chr4:3035108-3035127	MsG0480018297.01.T01:CDS	35.0%
	GTGACTTAAGATTGAAGGTA+GGG	-	Chr4:3035672-3035691	MsG0480018297.01.T01:intron	35.0%
	TATATGTACCTTGGCAACAA+TGG	+	Chr4:3038390-3038409	None:intergenic	35.0%
	TATCCATGATCAATGAGTGA+GGG	-	Chr4:3038612-3038631	MsG0480018297.01.T01:five_prime_UTR	35.0%
	TATCTGCACGAATATGGATA+AGG	+	Chr4:3035772-3035791	None:intergenic	35.0%
	TATTTATATGCTCAACAGCC+CGG	+	Chr4:3036740-3036759	None:intergenic	35.0%
	TCGAAGTGACTTAAGATTGA+AGG	-	Chr4:3035667-3035686	MsG0480018297.01.T01:intron	35.0%
	TGAAAAGAAATCATTGTCGG+CGG	-	Chr4:3037189-3037208	MsG0480018297.01.T01:intron	35.0%
	TGATACCATCTTAGAAATCG+TGG	+	Chr4:3036611-3036630	None:intergenic	35.0%
	TGTAGCTTAAGCAAAGTGAA+AGG	-	Chr4:3037151-3037170	MsG0480018297.01.T01:intron	35.0%
	TTATCCATGGATATCCATTC+AGG	-	Chr4:3036278-3036297	MsG0480018297.01.T01:intron	35.0%
	TTCTATCAGGTATCCACTAT+CGG	-	Chr4:3036855-3036874	MsG0480018297.01.T01:intron	35.0%
!	AATGCAAATGCCTTTGGAAA+GGG	+	Chr4:3037243-3037262	None:intergenic	35.0%
!	CCACTTTCACTTCCTTTTAA+AGG	-	Chr4:3036084-3036103	MsG0480018297.01.T01:intron	35.0%
!	CCTTTAAAAGGAAGTGAAAG+TGG	+	Chr4:3036087-3036106	None:intergenic	35.0%
!	TAATGCAAATGCCTTTGGAA+AGG	+	Chr4:3037244-3037263	None:intergenic	35.0%
!	TACCATTTTGTTCCTCACAT+TGG	+	Chr4:3038274-3038293	None:intergenic	35.0%
!	TCTAAGATGGTATCAGAGTT+GGG	-	Chr4:3036616-3036635	MsG0480018297.01.T01:intron	35.0%
!	TGAAGAATGAAAGTACAGTG+TGG	+	Chr4:3034824-3034843	None:intergenic	35.0%
!	TGTATGTGATGATGATGATG+AGG	-	Chr4:3035087-3035106	MsG0480018297.01.T01:CDS	35.0%
!	TTGGCCTTCTAATTCTGATT+TGG	-	Chr4:3034904-3034923	MsG0480018297.01.T01:CDS	35.0%
!!	ACCTTAAAAACCGGTTTTGT+GGG	-	Chr4:3036569-3036588	MsG0480018297.01.T01:intron	35.0%
!!	GTTTGGTTTGTGGACATAAA+TGG	+	Chr4:3036889-3036908	None:intergenic	35.0%
!!	TTCTAAGATGGTATCAGAGT+TGG	-	Chr4:3036615-3036634	MsG0480018297.01.T01:intron	35.0%
	AAAAGGAAGTGAAAGTGGTC+AGG	+	Chr4:3036082-3036101	None:intergenic	40.0%
	AAACAAGAGAATGGTGGTTG+AGG	+	Chr4:3034727-3034746	None:intergenic	40.0%
	AAAGCCATATATCCTCGCAT+GGG	+	Chr4:3035609-3035628	None:intergenic	40.0%
	AACGGATATCTGCACGAATA+TGG	+	Chr4:3035778-3035797	None:intergenic	40.0%
	AACGTTGACGCCTAAATCAT+TGG	-	Chr4:3037527-3037546	MsG0480018297.01.T01:intron	40.0%
	ACCATCTTAGAAATCGTGGT+TGG	+	Chr4:3036607-3036626	None:intergenic	40.0%
	ACGAACCTTCTTTAAGCCTA+TGG	+	Chr4:3035209-3035228	None:intergenic	40.0%
	ATCCATGATCAATGAGTGAG+GGG	-	Chr4:3038613-3038632	MsG0480018297.01.T01:five_prime_UTR	40.0%
	ATGCGAGGATATATGGCTTT+CGG	-	Chr4:3035609-3035628	MsG0480018297.01.T01:intron	40.0%
	CCATACCCGTTATTTAATCG+AGG	+	Chr4:3036182-3036201	None:intergenic	40.0%
	CTAACACAATCCCACAAAAC+CGG	+	Chr4:3036582-3036601	None:intergenic	40.0%
	CTTAATGGTATATGCACCTG+CGG	+	Chr4:3036209-3036228	None:intergenic	40.0%
	CTTGAATTCACATCTTGTCG+CGG	+	Chr4:3037604-3037623	None:intergenic	40.0%
	GAAAAGAAATCATTGTCGGC+GGG	-	Chr4:3037190-3037209	MsG0480018297.01.T01:intron	40.0%
	GATAAAATCCACCACGGATA+CGG	+	Chr4:3036312-3036331	None:intergenic	40.0%
	GCAATCCTCACCTCATAAAT+TGG	-	Chr4:3036991-3037010	MsG0480018297.01.T01:intron	40.0%
	GCAATCCTCACCTTAAAAAC+CGG	-	Chr4:3036560-3036579	MsG0480018297.01.T01:intron	40.0%
	GGATACAGATGCAGACTTAA+TGG	+	Chr4:3036224-3036243	None:intergenic	40.0%
	GGTTGTGAAGGTGAGTAATA+TGG	+	Chr4:3034706-3034725	None:intergenic	40.0%
	GGTTTGTGGACATAAATGGT+GGG	+	Chr4:3036885-3036904	None:intergenic	40.0%
	GTCCAATGTGAGGAACAAAA+TGG	-	Chr4:3038269-3038288	MsG0480018297.01.T01:intron	40.0%
	TAACAACACTCACAGTACTG+AGG	-	Chr4:3036116-3036135	MsG0480018297.01.T01:intron	40.0%
	TCTATCAGGTATCCACTATC+GGG	-	Chr4:3036856-3036875	MsG0480018297.01.T01:intron	40.0%
	TGGGTTAAAGTTTGCGTTGT+GGG	-	Chr4:3036635-3036654	MsG0480018297.01.T01:intron	40.0%
	TTCGTGGATAAAATCCACCA+CGG	+	Chr4:3036318-3036337	None:intergenic	40.0%
	TTGGGTTAAAGTTTGCGTTG+TGG	-	Chr4:3036634-3036653	MsG0480018297.01.T01:intron	40.0%
	TTTGCATCCGTGAATATTCG+TGG	+	Chr4:3036334-3036353	None:intergenic	40.0%
!	ATGCAAATGCCTTTGGAAAG+GGG	+	Chr4:3037242-3037261	None:intergenic	40.0%
!	ATGGATTTTGCGGTATCAAC+GGG	-	Chr4:3035811-3035830	MsG0480018297.01.T01:intron	40.0%
!	CATGGATTTTGCGGTATCAA+CGG	-	Chr4:3035810-3035829	MsG0480018297.01.T01:intron	40.0%
!	GAAAGTACAGTGTGGATAAG+AGG	+	Chr4:3034816-3034835	None:intergenic	40.0%
!	GTGAGTTTAGAGTCCTACAT+CGG	-	Chr4:3036932-3036951	MsG0480018297.01.T01:intron	40.0%
!	TCCAACCACGATTTCTAAGA+TGG	-	Chr4:3036603-3036622	MsG0480018297.01.T01:intron	40.0%
!	TCCATGGATTACCCACTAAA+CGG	+	Chr4:3035796-3035815	None:intergenic	40.0%
!	TGGGTAATCCATGGATTTTG+CGG	-	Chr4:3035801-3035820	MsG0480018297.01.T01:intron	40.0%
!	TGGTTTGTGGACATAAATGG+TGG	+	Chr4:3036886-3036905	None:intergenic	40.0%
!	TTTTTCTCCTTCGTTCATCG+AGG	+	Chr4:3037803-3037822	None:intergenic	40.0%
!!	CACCTTAAAAACCGGTTTTG+TGG	-	Chr4:3036568-3036587	MsG0480018297.01.T01:intron	40.0%
!!	CCTCGATTAAATAACGGGTA+TGG	-	Chr4:3036179-3036198	MsG0480018297.01.T01:intron	40.0%
!!	GATAGTGGATACCTGATAGA+AGG	+	Chr4:3036856-3036875	None:intergenic	40.0%
!!	GGTTGGTTAGTATTTTGAGC+AGG	+	Chr4:3038298-3038317	None:intergenic	40.0%
!!	TCCCACAAAACCGGTTTTTA+AGG	+	Chr4:3036573-3036592	None:intergenic	40.0%
!!!	CAAAACCGGTTTTTAAGGTG+AGG	+	Chr4:3036568-3036587	None:intergenic	40.0%
	AACACTCTTGCTTTGACCTC+TGG	+	Chr4:3038227-3038246	None:intergenic	45.0%
	AAGCCATATATCCTCGCATG+GGG	+	Chr4:3035608-3035627	None:intergenic	45.0%
	AATGGTGGTTGAGGTTGTGA+AGG	+	Chr4:3034718-3034737	None:intergenic	45.0%
	ACTCGTCATAGTCCAATGTG+AGG	-	Chr4:3038259-3038278	MsG0480018297.01.T01:intron	45.0%
	AGACGCCATAGGCTTAAAGA+AGG	-	Chr4:3035201-3035220	MsG0480018297.01.T01:CDS	45.0%
	AGAGAGCTAGAACAAGAAGC+TGG	-	Chr4:3038125-3038144	MsG0480018297.01.T01:CDS	45.0%
	AGAGCCTCTTCTAGATCCAT+TGG	-	Chr4:3036826-3036845	MsG0480018297.01.T01:intron	45.0%
	AGTACAGTGTGGATAAGAGG+TGG	+	Chr4:3034813-3034832	None:intergenic	45.0%
	AGTGGATACCTGATAGAAGG+TGG	+	Chr4:3036853-3036872	None:intergenic	45.0%
	ATACCCCATGCGAGGATATA+TGG	-	Chr4:3035602-3035621	MsG0480018297.01.T01:intron	45.0%
	ATATGGTGAGTAGAGTCCTG+TGG	+	Chr4:3034689-3034708	None:intergenic	45.0%
	ATCTGTGAGCGTGGGTATTT+CGG	+	Chr4:3037503-3037522	None:intergenic	45.0%
	CAAACCAAACCCAATAGTGC+TGG	-	Chr4:3036899-3036918	MsG0480018297.01.T01:intron	45.0%
	CGGGTCAGTTATAAATGGAG+AGG	-	Chr4:3037209-3037228	MsG0480018297.01.T01:intron	45.0%
	CGTGCAGATATCCGTTTAGT+GGG	-	Chr4:3035782-3035801	MsG0480018297.01.T01:intron	45.0%
	CGTTGATACCGCAAAATCCA+TGG	+	Chr4:3035812-3035831	None:intergenic	45.0%
	GAAAGCCATATATCCTCGCA+TGG	+	Chr4:3035610-3035629	None:intergenic	45.0%
	GACCATGATCTATCCGATGT+AGG	+	Chr4:3036948-3036967	None:intergenic	45.0%
	GAGACCTGAATGGATATCCA+TGG	+	Chr4:3036285-3036304	None:intergenic	45.0%
	GATAATAGATACCCCATGCG+AGG	-	Chr4:3035594-3035613	MsG0480018297.01.T01:intron	45.0%
	GGATCTTTACCCCTTTCCAA+AGG	-	Chr4:3037230-3037249	MsG0480018297.01.T01:intron	45.0%
	GGGTTAAAGTTTGCGTTGTG+GGG	-	Chr4:3036636-3036655	MsG0480018297.01.T01:intron	45.0%
	GTACAGTGTGGATAAGAGGT+GGG	+	Chr4:3034812-3034831	None:intergenic	45.0%
	GTCCTACATCGGATAGATCA+TGG	-	Chr4:3036943-3036962	MsG0480018297.01.T01:intron	45.0%
	GTGATTTCGTCGTCTAAGTG+CGG	+	Chr4:3037656-3037675	None:intergenic	45.0%
	GTGTAGTTAGTCGTCTTGTG+TGG	+	Chr4:3037629-3037648	None:intergenic	45.0%
	TACCCCTCACTCATTGATCA+TGG	+	Chr4:3038618-3038637	None:intergenic	45.0%
	TCGTGCAGATATCCGTTTAG+TGG	-	Chr4:3035781-3035800	MsG0480018297.01.T01:intron	45.0%
	TGACATCACCATTGTTGCCA+AGG	-	Chr4:3038379-3038398	MsG0480018297.01.T01:CDS	45.0%
	TGGTCCAATGGATCTAGAAG+AGG	+	Chr4:3036833-3036852	None:intergenic	45.0%
	TTGTTTGTCTCACATCCCAC+AGG	-	Chr4:3036711-3036730	MsG0480018297.01.T01:CDS	45.0%
!	AACAATGGTGATGTCAAGGG+TGG	+	Chr4:3038375-3038394	None:intergenic	45.0%
!	GGCAACAATGGTGATGTCAA+GGG	+	Chr4:3038378-3038397	None:intergenic	45.0%
!	TCCGTTTAGTGGGTAATCCA+TGG	-	Chr4:3035792-3035811	MsG0480018297.01.T01:intron	45.0%
!	TGGCAACAATGGTGATGTCA+AGG	+	Chr4:3038379-3038398	None:intergenic	45.0%
!	TTGTGGACATAAATGGTGGG+CGG	+	Chr4:3036882-3036901	None:intergenic	45.0%
!!	CGGTTTTGTGGGATTGTGTT+AGG	-	Chr4:3036580-3036599	MsG0480018297.01.T01:intron	45.0%
!!	GCACTATTGGGTTTGGTTTG+TGG	+	Chr4:3036899-3036918	None:intergenic	45.0%
!!	ATAATAATATATATTCTATT+TGG	-	Chr4:3038457-3038476	MsG0480018297.01.T01:five_prime_UTR	5.0%
!!!	ATTATTTTTTAAATAAAGTA+GGG	-	Chr4:3035544-3035563	MsG0480018297.01.T01:intron	5.0%
!!!	CTTTATTTTTATTAAATATA+AGG	+	Chr4:3035987-3036006	None:intergenic	5.0%
!!!	TATTATTTTTTAAATAAAGT+AGG	-	Chr4:3035543-3035562	MsG0480018297.01.T01:intron	5.0%
!!!	TTCATATAATATATTTTTAA+GGG	-	Chr4:3038425-3038444	MsG0480018297.01.T01:five_prime_UTR	5.0%
	ACGACCAGCACTATTGGGTT+TGG	+	Chr4:3036906-3036925	None:intergenic	50.0%
	AGTGTGGATAAGAGGTGGGT+TGG	+	Chr4:3034808-3034827	None:intergenic	50.0%
	CAGGAACTTTCAGACGCCAT+AGG	-	Chr4:3035190-3035209	MsG0480018297.01.T01:CDS	50.0%
	CCATTGGACCACCTTCTATC+AGG	-	Chr4:3036842-3036861	MsG0480018297.01.T01:intron	50.0%
	CCTGATAGAAGGTGGTCCAA+TGG	+	Chr4:3036845-3036864	None:intergenic	50.0%
	CCTTCACGACCAGCACTATT+GGG	+	Chr4:3036911-3036930	None:intergenic	50.0%
	CGTGGGTATTTCGGTGACTT+AGG	+	Chr4:3037494-3037513	None:intergenic	50.0%
	GATGAATTGAGTCGCTCCAC+AGG	-	Chr4:3034670-3034689	MsG0480018297.01.T01:three_prime_UTR	50.0%
	GGAGATCACAGAGATGTTGC+AGG	-	Chr4:3035171-3035190	MsG0480018297.01.T01:CDS	50.0%
	GTCGGCGGGTCAGTTATAAA+TGG	-	Chr4:3037204-3037223	MsG0480018297.01.T01:intron	50.0%
	TCTCACATCCCACAGGTTTC+CGG	-	Chr4:3036718-3036737	MsG0480018297.01.T01:CDS	50.0%
	TCTCCTTCGTTCATCGAGGA+GGG	+	Chr4:3037799-3037818	None:intergenic	50.0%
	TGGAGGAGTCGGGAGAAAAT+TGG	-	Chr4:3034885-3034904	MsG0480018297.01.T01:CDS	50.0%
	TTCTCCTTCGTTCATCGAGG+AGG	+	Chr4:3037800-3037819	None:intergenic	50.0%
!	CATCGAGGAGGGGTAGTTTT+AGG	+	Chr4:3037788-3037807	None:intergenic	50.0%
!	GATTTTCGTGTCTGCTCCGT+TGG	-	Chr4:3035728-3035747	MsG0480018297.01.T01:intron	50.0%
!	GTGTGGATAAGAGGTGGGTT+GGG	+	Chr4:3034807-3034826	None:intergenic	50.0%
!	TAACGGGTATGGATATCCGC+AGG	-	Chr4:3036190-3036209	MsG0480018297.01.T01:intron	50.0%
!!	CAATAGTGCTGGTCGTGAAG+GGG	-	Chr4:3036910-3036929	MsG0480018297.01.T01:intron	50.0%
!!	CCAATAGTGCTGGTCGTGAA+GGG	-	Chr4:3036909-3036928	MsG0480018297.01.T01:intron	50.0%
	AATCCACCACGGATACGGAG+CGG	+	Chr4:3036307-3036326	None:intergenic	55.0%
	ACGTTGCAGATCTGTGAGCG+TGG	+	Chr4:3037512-3037531	None:intergenic	55.0%
	AGTTTGCGTTGTGGGGACTC+AGG	-	Chr4:3036643-3036662	MsG0480018297.01.T01:intron	55.0%
	ATACGGAGCGGAGACCTGAA+TGG	+	Chr4:3036295-3036314	None:intergenic	55.0%
	CCCTTCACGACCAGCACTAT+TGG	+	Chr4:3036912-3036931	None:intergenic	55.0%
	CGTTGCAGATCTGTGAGCGT+GGG	+	Chr4:3037511-3037530	None:intergenic	55.0%
	CTCACATCCCACAGGTTTCC+GGG	-	Chr4:3036719-3036738	MsG0480018297.01.T01:CDS	55.0%
	CTCCTTCGTTCATCGAGGAG+GGG	+	Chr4:3037798-3037817	None:intergenic	55.0%
	TACCCCTCCTCGATGAACGA+AGG	-	Chr4:3037793-3037812	MsG0480018297.01.T01:intron	55.0%
	TCAACAGCCCGGAAACCTGT+GGG	+	Chr4:3036729-3036748	None:intergenic	55.0%
!!	CCCAATAGTGCTGGTCGTGA+AGG	-	Chr4:3036908-3036927	MsG0480018297.01.T01:intron	55.0%
	CTCAACAGCCCGGAAACCTG+TGG	+	Chr4:3036730-3036749	None:intergenic	60.0%
	ACCCACCCTCATGCCTAGCG+TGG	+	Chr4:3036670-3036689	None:intergenic	65.0%
	AGGTCTCCGCTCCGTATCCG+TGG	-	Chr4:3036298-3036317	MsG0480018297.01.T01:intron	65.0%
	CAGGCCCACGCTAGGCATGA+GGG	-	Chr4:3036662-3036681	MsG0480018297.01.T01:intron	65.0%
	CCCACCCTCATGCCTAGCGT+GGG	+	Chr4:3036669-3036688	None:intergenic	65.0%
	CCCACGCTAGGCATGAGGGT+GGG	-	Chr4:3036666-3036685	MsG0480018297.01.T01:intron	65.0%
	TCAGGCCCACGCTAGGCATG+AGG	-	Chr4:3036661-3036680	MsG0480018297.01.T01:intron	65.0%
	TCTCCGCTCCGTATCCGTGG+TGG	-	Chr4:3036301-3036320	MsG0480018297.01.T01:intron	65.0%
!!	AATGGTGGGCGGCCCGATAG+TGG	+	Chr4:3036871-3036890	None:intergenic	65.0%
	GCCCACGCTAGGCATGAGGG+TGG	-	Chr4:3036665-3036684	MsG0480018297.01.T01:intron	70.0%
	TGGGGACTCAGGCCCACGCT+AGG	-	Chr4:3036654-3036673	MsG0480018297.01.T01:intron	70.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	3034653	3038641	3034653	ID=MsG0480018297.01;Name=MsG0480018297.01
Chr4	mRNA	3034653	3038641	3034653	ID=MsG0480018297.01.T01;Parent=MsG0480018297.01;Name=MsG0480018297.01.T01;_AED=0.44;_eAED=0.44;_QI=231\|0.33\|0.5\|0.75\|0.33\|0.25\|4\|208\|245
Chr4	exon	3034653	3035232	3034653	ID=MsG0480018297.01.T01:exon:1309;Parent=MsG0480018297.01.T01
Chr4	exon	3036670	3036809	3036670	ID=MsG0480018297.01.T01:exon:1308;Parent=MsG0480018297.01.T01
Chr4	exon	3038068	3038251	3038068	ID=MsG0480018297.01.T01:exon:1307;Parent=MsG0480018297.01.T01
Chr4	exon	3038369	3038641	3038369	ID=MsG0480018297.01.T01:exon:1306;Parent=MsG0480018297.01.T01
Chr4	five_prime_UTR	3038411	3038641	3038411	ID=MsG0480018297.01.T01:five_prime_utr;Parent=MsG0480018297.01.T01
Chr4	CDS	3038369	3038410	3038369	ID=MsG0480018297.01.T01:cds;Parent=MsG0480018297.01.T01
Chr4	CDS	3038068	3038251	3038068	ID=MsG0480018297.01.T01:cds;Parent=MsG0480018297.01.T01
Chr4	CDS	3036670	3036809	3036670	ID=MsG0480018297.01.T01:cds;Parent=MsG0480018297.01.T01
Chr4	CDS	3034861	3035232	3034861	ID=MsG0480018297.01.T01:cds;Parent=MsG0480018297.01.T01
Chr4	three_prime_UTR	3034653	3034860	3034653	ID=MsG0480018297.01.T01:three_prime_utr;Parent=MsG0480018297.01.T01

Gene Sequence

>MsG0480018297.01.T01

ATGGAGGAGTCGGGAGAAAATTGGCCTTCTAATTCTGATTTGGAAATAAGTGATGAAGCCATTCTTAAAGATGGAGAATCTGAATCTGTATGTGATGATGATGATGAGGATCATTATTGTGAAGATCAGGTTGGAGATCCCACAGATAATACAAAGGAAAACAATGAGAAGGAGATCACAGAGATGTTGCAGGAACTTTCAGACGCCATAGGCTTAAAGAAGGTTCGTAACGATGAAGTGAAGTCTCTTAAAAGTCATATATCTGATAGAAGATGGTGTGACAATGTGTTTATAAGTGAGAGCAATCCTCACCTTAAAAACCGGTTTTGTGGGATTGTGTTAGGTCCAACCACGATTTCTAAGATGCTGGACGAAATTTCGATCATAGAGAAAGCTAGAGACTATGTAGCAACACTTCAAGAACGTGTGAGAGAGCTAGAACAAGAAGCTGGATTCAACATTTGTAACGAAAGGATCAAACTGAGTTCAAATTACTACAATTGTGGAACAAGTGATATAATTACTCTTCCAGAGGTCAAAGCAAGAGTGTTACAAAAAGTTGTACTCGTCATAGTCCAATGTGAGGAACAAAATGGTATCCTGCTCAAAATACTAACCAACCTTGAAAATCTTCATCTTTCAGTAGTTAACAGTAGAGTCTTGAATTTTGGAAAATCCACCCTTGACATCACCATTGTTGCCAAGGTACATATACAAGTCAATATATACTTCATATAA

Protein sequence

>MsG0480018297.01.T01

MEESGENWPSNSDLEISDEAILKDGESESVCDDDDEDHYCEDQVGDPTDNTKENNEKEITEMLQELSDAIGLKKVRNDEVKSLKSHISDRRWCDNVFISESNPHLKNRFCGIVLGPTTISKMLDEISIIEKARDYVATLQERVRELEQEAGFNICNERIKLSSNYYNCGTSDIITLPEVKARVLQKVVLVIVQCEEQNGILLKILTNLENLHLSVVNSRVLNFGKSTLDITIVAKVHIQVNIYFI*