Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047223.01.T01 | XP_003609850.1 | 96.585 | 205 | 7 | 0 | 1 | 205 | 61 | 265 | 7.15E-143 | 410 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047223.01.T01 | G7JQ18 | 96.585 | 205 | 7 | 0 | 1 | 205 | 61 | 265 | 3.42e-143 | 410 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048701.01 | MsG0880047223.01 | 0.832815 | 7.621595e-56 | 8.614826e-53 |
| MsG0080048781.01 | MsG0880047223.01 | 0.848124 | 7.485303e-60 | 1.359608e-56 |
| MsG0080048996.01 | MsG0880047223.01 | 0.800795 | 1.218550e-48 | 5.725386e-46 |
| MsG0180000076.01 | MsG0880047223.01 | 0.846397 | 2.228952e-59 | 3.831592e-56 |
| MsG0180000111.01 | MsG0880047223.01 | 0.821499 | 3.909531e-53 | 3.185928e-50 |
| MsG0180000353.01 | MsG0880047223.01 | 0.811539 | 6.654838e-51 | 4.130493e-48 |
| MsG0180000360.01 | MsG0880047223.01 | 0.825895 | 3.653090e-54 | 3.373493e-51 |
| MsG0180000373.01 | MsG0880047223.01 | 0.826557 | 2.541822e-54 | 2.391783e-51 |
| MsG0180001293.01 | MsG0880047223.01 | 0.812281 | 4.587830e-51 | 2.904852e-48 |
| MsG0180002397.01 | MsG0880047223.01 | 0.809601 | 1.745233e-50 | 1.029199e-47 |
| MsG0180002421.01 | MsG0880047223.01 | 0.801612 | 8.295623e-49 | 3.978930e-46 |
| MsG0880044989.01 | MsG0880047223.01 | 0.805225 | 1.479148e-49 | 7.782719e-47 |
| MsG0880045584.01 | MsG0880047223.01 | 0.809718 | 1.647619e-50 | 9.746057e-48 |
| MsG0880045612.01 | MsG0880047223.01 | 0.814772 | 1.299662e-51 | 8.799046e-49 |
| MsG0880046208.01 | MsG0880047223.01 | 0.802254 | 6.122931e-49 | 2.985286e-46 |
| MsG0880046431.01 | MsG0880047223.01 | 0.844207 | 8.722363e-59 | 1.398083e-55 |
| MsG0880046885.01 | MsG0880047223.01 | 0.810832 | 9.473245e-51 | 5.770934e-48 |
| MsG0880047223.01 | MsG0880047226.01 | 0.837463 | 5.122075e-57 | 6.658738e-54 |
| MsG0880047223.01 | MsG0880047431.01 | 0.823170 | 1.599846e-53 | 1.367136e-50 |
| MsG0880047223.01 | MsG0880047466.01 | 0.854704 | 1.030647e-61 | 2.322158e-58 |
| MsG0880047223.01 | MsG0880047649.01 | 0.831955 | 1.244387e-55 | 1.371327e-52 |
| MsG0180003427.01 | MsG0880047223.01 | 0.820135 | 8.050528e-53 | 6.317252e-50 |
| MsG0180003485.01 | MsG0880047223.01 | 0.827814 | 1.270825e-54 | 1.240262e-51 |
| MsG0180003658.01 | MsG0880047223.01 | 0.835678 | 1.459229e-56 | 1.797331e-53 |
| MsG0180003698.01 | MsG0880047223.01 | 0.821446 | 4.020540e-53 | 3.271669e-50 |
| MsG0180003796.01 | MsG0880047223.01 | 0.881023 | 3.386505e-70 | 1.952225e-66 |
| MsG0180003857.01 | MsG0880047223.01 | 0.838785 | 2.339131e-57 | 3.166341e-54 |
| MsG0180004126.01 | MsG0880047223.01 | 0.872977 | 2.099362e-67 | 8.957365e-64 |
| MsG0180004185.01 | MsG0880047223.01 | 0.816104 | 6.568495e-52 | 4.612104e-49 |
| MsG0180004299.01 | MsG0880047223.01 | 0.849436 | 3.238444e-60 | 6.138631e-57 |
| MsG0180004806.01 | MsG0880047223.01 | 0.808204 | 3.474825e-50 | 1.974718e-47 |
| MsG0180005002.01 | MsG0880047223.01 | 0.816554 | 5.207854e-52 | 3.701843e-49 |
| MsG0180005435.01 | MsG0880047223.01 | 0.805352 | 1.391593e-49 | 7.345895e-47 |
| MsG0180005835.01 | MsG0880047223.01 | 0.886393 | 3.557301e-72 | 2.536421e-68 |
| MsG0180006133.01 | MsG0880047223.01 | 0.813124 | 3.000314e-51 | 1.942484e-48 |
| MsG0780040364.01 | MsG0880047223.01 | 0.852459 | 4.553233e-61 | 9.520159e-58 |
| MsG0780040533.01 | MsG0880047223.01 | 0.803319 | 3.689023e-49 | 1.848113e-46 |
| MsG0780040543.01 | MsG0880047223.01 | 0.816867 | 4.432420e-52 | 3.177886e-49 |
| MsG0780040564.01 | MsG0880047223.01 | 0.802928 | 4.444804e-49 | 2.204615e-46 |
| MsG0780040600.01 | MsG0880047223.01 | 0.828624 | 8.105942e-55 | 8.100693e-52 |
| MsG0780040620.01 | MsG0880047223.01 | 0.801874 | 7.328306e-49 | 3.538748e-46 |
| MsG0780040828.01 | MsG0880047223.01 | 0.813794 | 2.136670e-51 | 1.408893e-48 |
| MsG0780041132.01 | MsG0880047223.01 | 0.825677 | 4.114273e-54 | 3.775714e-51 |
| MsG0780041141.01 | MsG0880047223.01 | 0.830037 | 3.680071e-55 | 3.832382e-52 |
| MsG0780041143.01 | MsG0880047223.01 | 0.861862 | 7.614285e-64 | 2.185242e-60 |
| MsG0780041340.01 | MsG0880047223.01 | 0.822792 | 1.960104e-53 | 1.657183e-50 |
| MsG0780041756.01 | MsG0880047223.01 | 0.837869 | 4.028205e-57 | 5.302804e-54 |
| MsG0880041837.01 | MsG0880047223.01 | 0.812132 | 4.944611e-51 | 3.118021e-48 |
| MsG0880042366.01 | MsG0880047223.01 | 0.802630 | 5.121021e-49 | 2.520814e-46 |
| MsG0880042906.01 | MsG0880047223.01 | 0.822769 | 1.984096e-53 | 1.676487e-50 |
| MsG0880042927.01 | MsG0880047223.01 | 0.861207 | 1.206342e-63 | 3.386427e-60 |
| MsG0880042929.01 | MsG0880047223.01 | 0.813960 | 1.964432e-51 | 1.301116e-48 |
| MsG0880043297.01 | MsG0880047223.01 | 0.812002 | 5.278628e-51 | 3.316844e-48 |
| MsG0880043516.01 | MsG0880047223.01 | 0.820753 | 5.805725e-53 | 4.634139e-50 |
| MsG0280010579.01 | MsG0880047223.01 | 0.813440 | 2.556759e-51 | 1.669821e-48 |
| MsG0280010795.01 | MsG0880047223.01 | 0.811945 | 5.431451e-51 | 3.407595e-48 |
| MsG0280010868.01 | MsG0880047223.01 | 0.808855 | 2.523241e-50 | 1.458525e-47 |
| MsG0280011154.01 | MsG0880047223.01 | 0.822465 | 2.334975e-53 | 1.955897e-50 |
| MsG0280011331.01 | MsG0880047223.01 | 0.812920 | 3.324626e-51 | 2.140811e-48 |
| MsG0380012472.01 | MsG0880047223.01 | 0.830875 | 2.295819e-55 | 2.450108e-52 |
| MsG0380012788.01 | MsG0880047223.01 | 0.816712 | 4.799925e-52 | 3.426730e-49 |
| MsG0380012808.01 | MsG0880047223.01 | 0.800674 | 1.289852e-48 | 6.042084e-46 |
| MsG0380014171.01 | MsG0880047223.01 | 0.821754 | 3.412230e-53 | 2.800802e-50 |
| MsG0380014754.01 | MsG0880047223.01 | 0.833007 | 6.827327e-56 | 7.762182e-53 |
| MsG0680031616.01 | MsG0880047223.01 | 0.830673 | 2.573098e-55 | 2.729799e-52 |
| MsG0680033696.01 | MsG0880047223.01 | 0.812327 | 4.482155e-51 | 2.841490e-48 |
| MsG0480018526.01 | MsG0880047223.01 | 0.837973 | 3.788251e-57 | 5.002948e-54 |
| MsG0480018785.01 | MsG0880047223.01 | 0.811778 | 5.904589e-51 | 3.687954e-48 |
| MsG0480018885.01 | MsG0880047223.01 | 0.830060 | 3.632472e-55 | 3.785330e-52 |
| MsG0480018918.01 | MsG0880047223.01 | 0.820122 | 8.104321e-53 | 6.357399e-50 |
| MsG0480019588.01 | MsG0880047223.01 | 0.846564 | 2.007082e-59 | 3.468711e-56 |
| MsG0480019635.01 | MsG0880047223.01 | 0.873873 | 1.048728e-67 | 4.623030e-64 |
| MsG0480019699.01 | MsG0880047223.01 | 0.819613 | 1.059573e-52 | 8.193137e-50 |
| MsG0480020263.01 | MsG0880047223.01 | 0.816670 | 4.906517e-52 | 3.498823e-49 |
| MsG0480020856.01 | MsG0880047223.01 | 0.804125 | 2.510002e-49 | 1.283799e-46 |
| MsG0480020965.01 | MsG0880047223.01 | 0.828407 | 9.146602e-55 | 9.083596e-52 |
| MsG0480021013.01 | MsG0880047223.01 | 0.860259 | 2.340291e-63 | 6.357746e-60 |
| MsG0480021156.01 | MsG0880047223.01 | 0.863641 | 2.152923e-64 | 6.573235e-61 |
| MsG0480022331.01 | MsG0880047223.01 | 0.830228 | 3.304903e-55 | 3.461118e-52 |
| MsG0480022559.01 | MsG0880047223.01 | 0.829110 | 6.181839e-55 | 6.266248e-52 |
| MsG0480023064.01 | MsG0880047223.01 | 0.830303 | 3.169236e-55 | 3.325816e-52 |
| MsG0480023194.01 | MsG0880047223.01 | 0.835611 | 1.517481e-56 | 1.865223e-53 |
| MsG0480023262.01 | MsG0880047223.01 | 0.813074 | 3.077063e-51 | 1.989529e-48 |
| MsG0480023296.01 | MsG0880047223.01 | 0.844618 | 6.762553e-59 | 1.097932e-55 |
| MsG0480023450.01 | MsG0880047223.01 | 0.813047 | 3.118832e-51 | 2.015142e-48 |
| MsG0480023458.01 | MsG0880047223.01 | 0.804850 | 1.772416e-49 | 9.236859e-47 |
| MsG0480023484.01 | MsG0880047223.01 | 0.803174 | 3.953761e-49 | 1.973453e-46 |
| MsG0480023532.01 | MsG0880047223.01 | 0.808725 | 2.690102e-50 | 1.549592e-47 |
| MsG0480023672.01 | MsG0880047223.01 | 0.844103 | 9.299082e-59 | 1.485714e-55 |
| MsG0580024489.01 | MsG0880047223.01 | 0.805511 | 1.288519e-49 | 6.829540e-47 |
| MsG0580024657.01 | MsG0880047223.01 | 0.835393 | 1.722787e-56 | 2.103955e-53 |
| MsG0580024787.01 | MsG0880047223.01 | 0.847422 | 1.168430e-59 | 2.075700e-56 |
| MsG0580024813.01 | MsG0880047223.01 | 0.833141 | 6.322142e-56 | 7.216899e-53 |
| MsG0580024954.01 | MsG0880047223.01 | 0.833789 | 4.358844e-56 | 5.072993e-53 |
| MsG0580025056.01 | MsG0880047223.01 | 0.830158 | 3.437682e-55 | 3.592735e-52 |
| MsG0580025093.01 | MsG0880047223.01 | 0.870112 | 1.865580e-66 | 7.172029e-63 |
| MsG0580025385.01 | MsG0880047223.01 | 0.844534 | 7.123773e-59 | 1.153586e-55 |
| MsG0580025464.01 | MsG0880047223.01 | 0.819453 | 1.152372e-52 | 8.871702e-50 |
| MsG0580025515.01 | MsG0880047223.01 | 0.881187 | 2.957118e-70 | 1.715644e-66 |
| MsG0580025775.01 | MsG0880047223.01 | 0.832179 | 1.095519e-55 | 1.215102e-52 |
| MsG0580025822.01 | MsG0880047223.01 | 0.807324 | 5.343908e-50 | 2.967726e-47 |
| MsG0380015161.01 | MsG0880047223.01 | 0.850349 | 1.798265e-60 | 3.512112e-57 |
| MsG0380015394.01 | MsG0880047223.01 | 0.855916 | 4.574370e-62 | 1.073023e-58 |
| MsG0380015548.01 | MsG0880047223.01 | 0.825539 | 4.435751e-54 | 4.054684e-51 |
| MsG0380015552.01 | MsG0880047223.01 | 0.816574 | 5.154828e-52 | 3.666286e-49 |
| MsG0380015816.01 | MsG0880047223.01 | 0.806440 | 8.219389e-50 | 4.461440e-47 |
| MsG0380015954.01 | MsG0880047223.01 | 0.822949 | 1.801860e-53 | 1.530175e-50 |
| MsG0380016091.01 | MsG0880047223.01 | 0.801467 | 8.881618e-49 | 4.244632e-46 |
| MsG0380016217.01 | MsG0880047223.01 | 0.846578 | 1.989434e-59 | 3.439699e-56 |
| MsG0380016703.01 | MsG0880047223.01 | 0.807446 | 5.035956e-50 | 2.805527e-47 |
| MsG0380017164.01 | MsG0880047223.01 | 0.824691 | 7.037172e-54 | 6.279724e-51 |
| MsG0380017239.01 | MsG0880047223.01 | 0.860635 | 1.800761e-63 | 4.955582e-60 |
| MsG0380017361.01 | MsG0880047223.01 | 0.848288 | 6.742416e-60 | 1.231404e-56 |
| MsG0380017632.01 | MsG0880047223.01 | 0.803255 | 3.803771e-49 | 1.902499e-46 |
| MsG0580027974.01 | MsG0880047223.01 | 0.810618 | 1.054061e-50 | 6.384327e-48 |
| MsG0580028756.01 | MsG0880047223.01 | 0.842946 | 1.895536e-58 | 2.919684e-55 |
| MsG0580028923.01 | MsG0880047223.01 | 0.812509 | 4.089741e-51 | 2.605314e-48 |
| MsG0580028927.01 | MsG0880047223.01 | 0.809058 | 2.282100e-50 | 1.326353e-47 |
| MsG0580029123.01 | MsG0880047223.01 | 0.815315 | 9.848063e-52 | 6.766714e-49 |
| MsG0580029261.01 | MsG0880047223.01 | 0.814039 | 1.886799e-51 | 1.252433e-48 |
| MsG0580029298.01 | MsG0880047223.01 | 0.810190 | 1.303545e-50 | 7.806196e-48 |
| MsG0580029591.01 | MsG0880047223.01 | 0.827012 | 1.979007e-54 | 1.886804e-51 |
| MsG0280006540.01 | MsG0880047223.01 | 0.853841 | 1.829570e-61 | 4.006842e-58 |
| MsG0280006563.01 | MsG0880047223.01 | 0.848967 | 4.371252e-60 | 8.161380e-57 |
| MsG0280006884.01 | MsG0880047223.01 | 0.812674 | 3.765255e-51 | 2.408927e-48 |
| MsG0280007359.01 | MsG0880047223.01 | 0.844128 | 9.157079e-59 | 1.464072e-55 |
| MsG0280007419.01 | MsG0880047223.01 | 0.825928 | 3.586268e-54 | 3.314964e-51 |
| MsG0280007761.01 | MsG0880047223.01 | 0.857965 | 1.139162e-62 | 2.861797e-59 |
| MsG0280007983.01 | MsG0880047223.01 | 0.808787 | 2.608540e-50 | 1.505111e-47 |
| MsG0280008407.01 | MsG0880047223.01 | 0.847899 | 8.636060e-60 | 1.557691e-56 |
| MsG0280009241.01 | MsG0880047223.01 | 0.800514 | 1.390571e-48 | 6.487371e-46 |
| MsG0280009752.01 | MsG0880047223.01 | 0.813849 | 2.078381e-51 | 1.372452e-48 |
| MsG0680035878.01 | MsG0880047223.01 | 0.801831 | 7.479434e-49 | 3.607669e-46 |
| MsG0780036360.01 | MsG0880047223.01 | 0.808103 | 3.649656e-50 | 2.068479e-47 |
| MsG0780038393.01 | MsG0880047223.01 | 0.801948 | 7.074895e-49 | 3.422581e-46 |
| MsG0780038402.01 | MsG0880047223.01 | 0.820712 | 5.933483e-53 | 4.730661e-50 |
| MsG0780038810.01 | MsG0880047223.01 | 0.815897 | 7.303181e-52 | 5.098682e-49 |
| MsG0780039533.01 | MsG0880047223.01 | 0.800640 | 1.310760e-48 | 6.134674e-46 |
| MsG0780039861.01 | MsG0880047223.01 | 0.877847 | 4.523996e-69 | 2.309534e-65 |
| MsG0780039878.01 | MsG0880047223.01 | 0.825672 | 4.126324e-54 | 3.786298e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047223.01.T01 | MTR_4g123700 | 96.585 | 205 | 7 | 0 | 1 | 205 | 61 | 265 | 8.66e-147 | 410 |
| MsG0880047223.01.T01 | MTR_4g105550 | 36.111 | 216 | 120 | 8 | 4 | 203 | 62 | 275 | 1.52e-35 | 127 |
| MsG0880047223.01.T01 | MTR_8g090055 | 28.436 | 211 | 130 | 7 | 3 | 203 | 63 | 262 | 1.50e-17 | 79.7 |
| MsG0880047223.01.T01 | MTR_5g037310 | 30.516 | 213 | 132 | 8 | 4 | 203 | 57 | 266 | 1.68e-17 | 79.3 |
| MsG0880047223.01.T01 | MTR_5g037310 | 30.516 | 213 | 132 | 8 | 4 | 203 | 57 | 266 | 2.07e-17 | 79.3 |
| MsG0880047223.01.T01 | MTR_2g023970 | 29.730 | 222 | 127 | 11 | 1 | 203 | 51 | 262 | 1.45e-14 | 71.6 |
| MsG0880047223.01.T01 | MTR_4g132450 | 30.097 | 206 | 119 | 11 | 6 | 194 | 71 | 268 | 1.42e-13 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047223.01.T01 | AT3G17350 | 62.673 | 217 | 71 | 3 | 1 | 208 | 58 | 273 | 1.56e-95 | 280 |
| MsG0880047223.01.T01 | AT3G17350 | 63.850 | 213 | 67 | 3 | 1 | 204 | 58 | 269 | 3.20e-95 | 280 |
| MsG0880047223.01.T01 | AT5G50290 | 32.579 | 221 | 130 | 8 | 4 | 207 | 52 | 270 | 1.90e-31 | 117 |
| MsG0880047223.01.T01 | AT1G10380 | 34.146 | 205 | 111 | 8 | 16 | 203 | 73 | 270 | 5.12e-23 | 94.7 |
| MsG0880047223.01.T01 | AT1G10380 | 34.146 | 205 | 111 | 8 | 16 | 203 | 73 | 270 | 5.45e-23 | 94.4 |
| MsG0880047223.01.T01 | AT1G11915 | 27.363 | 201 | 132 | 5 | 7 | 195 | 66 | 264 | 4.37e-15 | 73.2 |
Find 38 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTACTACTTGGTTTCTTATT+TGG | 0.088679 | 8:-83536360 | MsG0880047223.01.T01:CDS |
| GGTCCTTTGGATTGGGTTTA+TGG | 0.099494 | 8:-83536525 | MsG0880047223.01.T01:CDS |
| GTTTATGAGTATGAATATTT+TGG | 0.176981 | 8:-83536598 | MsG0880047223.01.T01:CDS |
| GTCCTTTGGATTGGGTTTAT+GGG | 0.234143 | 8:-83536524 | MsG0880047223.01.T01:CDS |
| TTATTCTTTCAAACTCCTTC+TGG | 0.239774 | 8:-83536951 | MsG0880047223.01.T01:CDS |
| CACACTTGTGATGAGCTTTA+TGG | 0.305918 | 8:-83536702 | MsG0880047223.01.T01:CDS |
| ATTGAGAGGTGTTGGTCCTT+TGG | 0.340253 | 8:-83536538 | MsG0880047223.01.T01:CDS |
| CAACTTTCACATCCAGTATC+AGG | 0.395675 | 8:+83536480 | None:intergenic |
| CACAATCTGGTGGGATTTGT+GGG | 0.413624 | 8:-83536455 | MsG0880047223.01.T01:CDS |
| TAAAACAAAGATATTTGTAA+TGG | 0.422370 | 8:+83536736 | None:intergenic |
| AGGTGTTGGTCCTTTGGATT+GGG | 0.424565 | 8:-83536532 | MsG0880047223.01.T01:CDS |
| GAGGTGTTGGTCCTTTGGAT+TGG | 0.424668 | 8:-83536533 | MsG0880047223.01.T01:CDS |
| ATGGAGCAAGTTGACATTGA+TGG | 0.431134 | 8:+83536873 | None:intergenic |
| TATTTGTAATGGTTAAGAAC+AGG | 0.444608 | 8:+83536747 | None:intergenic |
| ACACAATCTGGTGGGATTTG+TGG | 0.447706 | 8:-83536456 | MsG0880047223.01.T01:CDS |
| TGCTATTATCCCTCCATCAC+AGG | 0.479220 | 8:-83536808 | MsG0880047223.01.T01:CDS |
| GAATGTGGTAAGCTACTACT+TGG | 0.493321 | 8:-83536372 | MsG0880047223.01.T01:CDS |
| ATCATGATGAGGTTGAAGAA+TGG | 0.498307 | 8:+83536854 | None:intergenic |
| TGGAGCAAGTTGACATTGAT+GGG | 0.527863 | 8:+83536874 | None:intergenic |
| TGTGAAAGTTGTACACAATC+TGG | 0.535884 | 8:-83536468 | MsG0880047223.01.T01:CDS |
| AGTGATAAATTGAGAGGTGT+TGG | 0.537265 | 8:-83536546 | MsG0880047223.01.T01:CDS |
| CAAGTTTCACTAATTCAACA+AGG | 0.561620 | 8:-83536395 | MsG0880047223.01.T01:CDS |
| AAAACAGTGTCCTGTGATGG+AGG | 0.573412 | 8:+83536798 | None:intergenic |
| ACTAATTCAACAAGGGAATG+TGG | 0.573682 | 8:-83536387 | MsG0880047223.01.T01:CDS |
| ATCCCATAAACCCAATCCAA+AGG | 0.604071 | 8:+83536522 | None:intergenic |
| TGAACTTTGTAAGAACCAGA+AGG | 0.628532 | 8:+83536936 | None:intergenic |
| AAACAGTGTCCTGTGATGGA+GGG | 0.633615 | 8:+83536799 | None:intergenic |
| TTTAATAGTGATAAATTGAG+AGG | 0.647028 | 8:-83536552 | MsG0880047223.01.T01:CDS |
| TTATAGCTCGTGAAACAACA+AGG | 0.671730 | 8:+83536630 | None:intergenic |
| TGATGGGTCGTAGAGTACCA+TGG | 0.674328 | 8:+83536890 | None:intergenic |
| ACTTGTGATGAGCTTTATGG+TGG | 0.679797 | 8:-83536699 | MsG0880047223.01.T01:CDS |
| GGGTTTGATACTGAAACTGA+AGG | 0.680082 | 8:-83536435 | MsG0880047223.01.T01:CDS |
| AAGTTTCACTAATTCAACAA+GGG | 0.686221 | 8:-83536394 | MsG0880047223.01.T01:CDS |
| GAAAGTTGTACACAATCTGG+TGG | 0.691833 | 8:-83536465 | MsG0880047223.01.T01:CDS |
| GTCATGAGAAAATCATGATG+AGG | 0.693658 | 8:+83536843 | None:intergenic |
| AAAGTTGTACACAATCTGGT+GGG | 0.724462 | 8:-83536464 | MsG0880047223.01.T01:CDS |
| AGTTTGAAAGAATAACTCAG+TGG | 0.732725 | 8:+83536959 | None:intergenic |
| TAGCTCGTGAAACAACAAGG+TGG | 0.804087 | 8:+83536633 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAACAAAGATATTTGTAA+TGG | + | Chr8:83536595-83536614 | None:intergenic | 15.0% |
| !! | TTTAATAGTGATAAATTGAG+AGG | - | Chr8:83536776-83536795 | MsG0880047223.01.T01:CDS | 20.0% |
| !!! | GTTTATGAGTATGAATATTT+TGG | - | Chr8:83536730-83536749 | MsG0880047223.01.T01:CDS | 20.0% |
| !!! | TATCTTTGTTTTAACTTTGA+AGG | - | Chr8:83536602-83536621 | MsG0880047223.01.T01:CDS | 20.0% |
| ! | AAGTTTCACTAATTCAACAA+GGG | - | Chr8:83536934-83536953 | MsG0880047223.01.T01:CDS | 25.0% |
| ! | TATTTGTAATGGTTAAGAAC+AGG | + | Chr8:83536584-83536603 | None:intergenic | 25.0% |
| AGTTTGAAAGAATAACTCAG+TGG | + | Chr8:83536372-83536391 | None:intergenic | 30.0% | |
| CAAGTTTCACTAATTCAACA+AGG | - | Chr8:83536933-83536952 | MsG0880047223.01.T01:CDS | 30.0% | |
| CTACTACTTGGTTTCTTATT+TGG | - | Chr8:83536968-83536987 | MsG0880047223.01.T01:CDS | 30.0% | |
| TGACTACAACAAAAAAACCA+TGG | - | Chr8:83536421-83536440 | MsG0880047223.01.T01:CDS | 30.0% | |
| TTATTCTTTCAAACTCCTTC+TGG | - | Chr8:83536377-83536396 | MsG0880047223.01.T01:CDS | 30.0% | |
| !!! | GGTTTTTTTGTTGTAGTCAA+TGG | + | Chr8:83536420-83536439 | None:intergenic | 30.0% |
| AAAGTTGTACACAATCTGGT+GGG | - | Chr8:83536864-83536883 | MsG0880047223.01.T01:CDS | 35.0% | |
| ACTAATTCAACAAGGGAATG+TGG | - | Chr8:83536941-83536960 | MsG0880047223.01.T01:CDS | 35.0% | |
| ATCATGATGAGGTTGAAGAA+TGG | + | Chr8:83536477-83536496 | None:intergenic | 35.0% | |
| GTCATGAGAAAATCATGATG+AGG | + | Chr8:83536488-83536507 | None:intergenic | 35.0% | |
| TGAACTTTGTAAGAACCAGA+AGG | + | Chr8:83536395-83536414 | None:intergenic | 35.0% | |
| TGTGAAAGTTGTACACAATC+TGG | - | Chr8:83536860-83536879 | MsG0880047223.01.T01:CDS | 35.0% | |
| TTATAGCTCGTGAAACAACA+AGG | + | Chr8:83536701-83536720 | None:intergenic | 35.0% | |
| ! | AGTGATAAATTGAGAGGTGT+TGG | - | Chr8:83536782-83536801 | MsG0880047223.01.T01:CDS | 35.0% |
| ACAAAAACAGTGTCCTGTGA+TGG | + | Chr8:83536536-83536555 | None:intergenic | 40.0% | |
| ATCCCATAAACCCAATCCAA+AGG | + | Chr8:83536809-83536828 | None:intergenic | 40.0% | |
| CAACTTTCACATCCAGTATC+AGG | + | Chr8:83536851-83536870 | None:intergenic | 40.0% | |
| CACACTTGTGATGAGCTTTA+TGG | - | Chr8:83536626-83536645 | MsG0880047223.01.T01:CDS | 40.0% | |
| GAAAGTTGTACACAATCTGG+TGG | - | Chr8:83536863-83536882 | MsG0880047223.01.T01:CDS | 40.0% | |
| GAATGTGGTAAGCTACTACT+TGG | - | Chr8:83536956-83536975 | MsG0880047223.01.T01:CDS | 40.0% | |
| GGGTTTGATACTGAAACTGA+AGG | - | Chr8:83536893-83536912 | MsG0880047223.01.T01:CDS | 40.0% | |
| ! | ACTTGTGATGAGCTTTATGG+TGG | - | Chr8:83536629-83536648 | MsG0880047223.01.T01:CDS | 40.0% |
| ! | ATGGAGCAAGTTGACATTGA+TGG | + | Chr8:83536458-83536477 | None:intergenic | 40.0% |
| ! | ATGGTGGTTGCAATGCTTTT+AGG | - | Chr8:83536645-83536664 | MsG0880047223.01.T01:CDS | 40.0% |
| ! | GTCCTTTGGATTGGGTTTAT+GGG | - | Chr8:83536804-83536823 | MsG0880047223.01.T01:CDS | 40.0% |
| ! | TCTTTTAGTGTGCCTGATAC+TGG | - | Chr8:83536836-83536855 | MsG0880047223.01.T01:CDS | 40.0% |
| ! | TGGAGCAAGTTGACATTGAT+GGG | + | Chr8:83536457-83536476 | None:intergenic | 40.0% |
| ! | TGGTGGTTGCAATGCTTTTA+GGG | - | Chr8:83536646-83536665 | MsG0880047223.01.T01:CDS | 40.0% |
| AAAACAGTGTCCTGTGATGG+AGG | + | Chr8:83536533-83536552 | None:intergenic | 45.0% | |
| ACACAATCTGGTGGGATTTG+TGG | - | Chr8:83536872-83536891 | MsG0880047223.01.T01:CDS | 45.0% | |
| CACAATCTGGTGGGATTTGT+GGG | - | Chr8:83536873-83536892 | MsG0880047223.01.T01:CDS | 45.0% | |
| TAGCTCGTGAAACAACAAGG+TGG | + | Chr8:83536698-83536717 | None:intergenic | 45.0% | |
| TGCTATTATCCCTCCATCAC+AGG | - | Chr8:83536520-83536539 | MsG0880047223.01.T01:CDS | 45.0% | |
| ! | AAACAGTGTCCTGTGATGGA+GGG | + | Chr8:83536532-83536551 | None:intergenic | 45.0% |
| ! | GGTCCTTTGGATTGGGTTTA+TGG | - | Chr8:83536803-83536822 | MsG0880047223.01.T01:CDS | 45.0% |
| !! | AGGTGTTGGTCCTTTGGATT+GGG | - | Chr8:83536796-83536815 | MsG0880047223.01.T01:CDS | 45.0% |
| !! | ATTGAGAGGTGTTGGTCCTT+TGG | - | Chr8:83536790-83536809 | MsG0880047223.01.T01:CDS | 45.0% |
| ! | TGATGGGTCGTAGAGTACCA+TGG | + | Chr8:83536441-83536460 | None:intergenic | 50.0% |
| !! | GAGGTGTTGGTCCTTTGGAT+TGG | - | Chr8:83536795-83536814 | MsG0880047223.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 83536353 | 83536997 | 83536353 | ID=MsG0880047223.01;Name=MsG0880047223.01 |
| Chr8 | mRNA | 83536353 | 83536997 | 83536353 | ID=MsG0880047223.01.T01;Parent=MsG0880047223.01;Name=MsG0880047223.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|214 |
| Chr8 | exon | 83536353 | 83536997 | 83536353 | ID=MsG0880047223.01.T01:exon:20762;Parent=MsG0880047223.01.T01 |
| Chr8 | CDS | 83536353 | 83536997 | 83536353 | ID=MsG0880047223.01.T01:cds;Parent=MsG0880047223.01.T01 |
| Gene Sequence |
| Protein sequence |
