AlfalfaGEDB | MsG0580027826.01

M. sativa cultivar ZhongmuNo.1 / MsG0580027826.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027826.01.T01	XP_003615100.1	95.775	213	6	2	1	210	1	213	2.79E-142	405

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027826.01.T01	G7KB77	95.775	213	6	2	1	210	1	213	1.33e-142	405

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048065.01	MsG0580027826.01	0.814900	1.217543e-51	8.271607e-49
MsG0080048773.01	MsG0580027826.01	0.835703	1.438202e-56	1.772838e-53
MsG0080048878.01	MsG0580027826.01	0.809880	1.520549e-50	9.032651e-48
MsG0080049040.01	MsG0580027826.01	0.804894	1.734766e-49	9.051474e-47
MsG0080049068.01	MsG0580027826.01	0.807741	4.358934e-50	2.447118e-47
MsG0180000355.01	MsG0580027826.01	0.800498	1.401270e-48	6.534551e-46
MsG0180000404.01	MsG0580027826.01	0.812929	3.310484e-51	2.132177e-48
MsG0180000782.01	MsG0580027826.01	0.848723	5.108828e-60	9.463676e-57
MsG0180000784.01	MsG0580027826.01	0.817971	2.500680e-52	1.847826e-49
MsG0180000785.01	MsG0580027826.01	0.835584	1.541413e-56	1.893068e-53
MsG0180001085.01	MsG0580027826.01	0.817991	2.474672e-52	1.829695e-49
MsG0180001100.01	MsG0580027826.01	0.822150	2.762331e-53	2.293077e-50
MsG0180001270.01	MsG0580027826.01	0.812693	3.728211e-51	2.386492e-48
MsG0180001610.01	MsG0580027826.01	0.804695	1.909615e-49	9.911595e-47
MsG0180001727.01	MsG0580027826.01	0.800393	1.471641e-48	6.844671e-46
MsG0180002278.01	MsG0580027826.01	0.833295	5.789265e-56	6.638624e-53
MsG0180002279.01	MsG0580027826.01	0.826054	3.348183e-54	3.105875e-51
MsG0180002399.01	MsG0580027826.01	0.820522	6.561134e-53	5.203317e-50
MsG0180003353.01	MsG0580027826.01	0.842853	2.006761e-58	3.082190e-55
MsG0180003633.01	MsG0580027826.01	0.805033	1.622369e-49	8.494297e-47
MsG0180003741.01	MsG0580027826.01	0.837429	5.224057e-57	6.784232e-54
MsG0180003821.01	MsG0580027826.01	0.831256	1.850445e-55	1.997075e-52
MsG0180003885.01	MsG0580027826.01	0.827764	1.306723e-54	1.273452e-51
MsG0180004103.01	MsG0580027826.01	0.817379	3.399515e-52	2.471792e-49
MsG0180004192.01	MsG0580027826.01	0.802707	4.937717e-49	2.435413e-46
MsG0180004246.01	MsG0580027826.01	0.832537	8.933536e-56	1.001481e-52
MsG0180004493.01	MsG0580027826.01	0.819569	1.083930e-52	8.372024e-50
MsG0180004735.01	MsG0580027826.01	0.830008	3.739993e-55	3.891624e-52
MsG0180004814.01	MsG0580027826.01	0.812591	3.925519e-51	2.506013e-48
MsG0180005039.01	MsG0580027826.01	0.811952	5.411361e-51	3.395665e-48
MsG0180005113.01	MsG0580027826.01	0.819048	1.424784e-52	1.084667e-49
MsG0180005153.01	MsG0580027826.01	0.820194	7.800928e-53	6.131299e-50
MsG0180005169.01	MsG0580027826.01	0.810256	1.261579e-50	7.568318e-48
MsG0180005807.01	MsG0580027826.01	0.843343	1.485857e-58	2.318063e-55
MsG0180006017.01	MsG0580027826.01	0.812377	4.371756e-51	2.775048e-48
MsG0180006031.01	MsG0580027826.01	0.839631	1.411685e-57	1.961082e-54
MsG0180006096.01	MsG0580027826.01	0.815147	1.072747e-51	7.336972e-49
MsG0280006309.01	MsG0580027826.01	0.811818	5.788714e-51	3.619334e-48
MsG0280010564.01	MsG0580027826.01	0.814878	1.230951e-51	8.357809e-49
MsG0280010569.01	MsG0580027826.01	0.841940	3.501885e-58	5.226859e-55
MsG0280011255.01	MsG0580027826.01	0.813197	2.890818e-51	1.875409e-48
MsG0380012208.01	MsG0580027826.01	0.800326	1.518582e-48	7.051187e-46
MsG0480018584.01	MsG0580027826.01	0.848228	7.006373e-60	1.276985e-56
MsG0480018850.01	MsG0580027826.01	0.808421	3.123038e-50	1.784916e-47
MsG0480018911.01	MsG0580027826.01	0.813183	2.912160e-51	1.888468e-48
MsG0480019711.01	MsG0580027826.01	0.824544	7.622900e-54	6.772926e-51
MsG0480019971.01	MsG0580027826.01	0.809074	2.264328e-50	1.316615e-47
MsG0480020024.01	MsG0580027826.01	0.819296	1.251569e-52	9.592424e-50
MsG0480020315.01	MsG0580027826.01	0.819140	1.357876e-52	1.036239e-49
MsG0480020408.01	MsG0580027826.01	0.813656	2.292286e-51	1.505830e-48
MsG0480020690.01	MsG0580027826.01	0.826308	2.914004e-54	2.722663e-51
MsG0480020783.01	MsG0580027826.01	0.824762	6.770409e-54	6.053776e-51
MsG0480020889.01	MsG0580027826.01	0.823511	1.331892e-53	1.149175e-50
MsG0480020890.01	MsG0580027826.01	0.828397	9.197466e-55	9.131481e-52
MsG0480021060.01	MsG0580027826.01	0.803332	3.667546e-49	1.837948e-46
MsG0480021381.01	MsG0580027826.01	0.820650	6.130955e-53	4.879335e-50
MsG0480022355.01	MsG0580027826.01	0.846023	2.817070e-59	4.784623e-56
MsG0480022537.01	MsG0580027826.01	0.843347	1.481788e-58	2.311971e-55
MsG0480022624.01	MsG0580027826.01	0.838951	2.119740e-57	2.883781e-54
MsG0480022647.01	MsG0580027826.01	0.820949	5.233181e-53	4.200349e-50
MsG0480023381.01	MsG0580027826.01	0.837019	6.654670e-57	8.534420e-54
MsG0480023384.01	MsG0580027826.01	0.821321	4.296619e-53	3.484165e-50
MsG0480023385.01	MsG0580027826.01	0.807583	4.710011e-50	2.633099e-47
MsG0480023553.01	MsG0580027826.01	0.816844	4.485446e-52	3.213870e-49
MsG0480023565.01	MsG0580027826.01	0.802187	6.319124e-49	3.075582e-46
MsG0480023739.01	MsG0580027826.01	0.803956	2.721817e-49	1.386167e-46
MsG0480023988.01	MsG0580027826.01	0.802285	6.031384e-49	2.943121e-46
MsG0480023992.01	MsG0580027826.01	0.814564	1.444808e-51	9.727975e-49
MsG0480024030.01	MsG0580027826.01	0.822324	2.517411e-53	2.100291e-50
MsG0580024569.01	MsG0580027826.01	0.800176	1.629535e-48	7.537069e-46
MsG0580025055.01	MsG0580027826.01	0.814458	1.524972e-51	1.023802e-48
MsG0580025411.01	MsG0580027826.01	0.807821	4.192239e-50	2.358342e-47
MsG0580025668.01	MsG0580027826.01	0.841249	5.328958e-58	7.783443e-55
MsG0380014926.01	MsG0580027826.01	0.814864	1.240155e-51	8.417049e-49
MsG0380015784.01	MsG0580027826.01	0.804994	1.653764e-49	8.650041e-47
MsG0380015896.01	MsG0580027826.01	0.810412	1.167450e-50	7.032239e-48
MsG0380016284.01	MsG0580027826.01	0.821543	3.818499e-53	3.115726e-50
MsG0380016540.01	MsG0580027826.01	0.807359	5.252919e-50	2.919747e-47
MsG0380016671.01	MsG0580027826.01	0.800053	1.726351e-48	7.960141e-46
MsG0380016864.01	MsG0580027826.01	0.838180	3.351848e-57	4.453675e-54
MsG0380017087.01	MsG0580027826.01	0.846059	2.754023e-59	4.682580e-56
MsG0380017202.01	MsG0580027826.01	0.808497	3.008939e-50	1.723104e-47
MsG0380017338.01	MsG0580027826.01	0.805346	1.395622e-49	7.366087e-47
MsG0380017423.01	MsG0580027826.01	0.810108	1.358099e-50	8.116072e-48
MsG0380017931.01	MsG0580027826.01	0.815609	8.468921e-52	5.866786e-49
MsG0380018037.01	MsG0580027826.01	0.825589	4.315707e-54	3.950690e-51
MsG0380018069.01	MsG0580027826.01	0.809990	1.439584e-50	8.576810e-48
MsG0480018200.01	MsG0580027826.01	0.810812	9.570534e-51	5.826797e-48
MsG0480018218.01	MsG0580027826.01	0.814302	1.651315e-51	1.103987e-48
MsG0480018423.01	MsG0580027826.01	0.834222	3.395085e-56	4.002809e-53
MsG0580027394.01	MsG0580027826.01	0.819821	9.496907e-53	7.386813e-50
MsG0580027655.01	MsG0580027826.01	0.896290	4.250337e-76	4.568951e-72
MsG0580027726.01	MsG0580027826.01	0.812424	4.269041e-51	2.713506e-48
MsG0580027763.01	MsG0580027826.01	0.821995	3.002006e-53	2.480985e-50
MsG0580027826.01	MsG0580027980.01	0.815001	1.155907e-51	7.875044e-49
MsG0580027826.01	MsG0580028024.01	0.810688	1.018033e-50	6.177734e-48
MsG0580027826.01	MsG0580028059.01	0.801080	1.065883e-48	5.044722e-46
MsG0580027826.01	MsG0580028407.01	0.821365	4.197812e-53	3.408111e-50
MsG0580027826.01	MsG0580028551.01	0.802543	5.338392e-49	2.621990e-46
MsG0580027826.01	MsG0580028723.01	0.810543	1.094129e-50	6.613452e-48
MsG0580027826.01	MsG0580029414.01	0.820257	7.545199e-53	5.940386e-50
MsG0580027826.01	MsG0580029529.01	0.850308	1.847338e-60	3.603286e-57
MsG0580027826.01	MsG0580029736.01	0.866801	2.186032e-65	7.463389e-62
MsG0580027826.01	MsG0580029879.01	0.806022	1.006327e-49	5.404664e-47
MsG0580027826.01	MsG0680030328.01	0.820476	6.723092e-53	5.325235e-50
MsG0580027826.01	MsG0680030515.01	0.827790	1.287847e-54	1.255996e-51
MsG0580027826.01	MsG0680030680.01	0.827619	1.415201e-54	1.373282e-51
MsG0580027826.01	MsG0680030784.01	0.852084	5.820938e-61	1.202715e-57
MsG0580027826.01	MsG0680030858.01	0.809516	1.820427e-50	1.071050e-47
MsG0580027826.01	MsG0680030895.01	0.836040	1.181236e-56	1.470834e-53
MsG0580027826.01	MsG0680030905.01	0.822709	2.049112e-53	1.728399e-50
MsG0580027826.01	MsG0680030908.01	0.810991	8.750980e-51	5.354136e-48
MsG0580027826.01	MsG0680031010.01	0.803074	4.146735e-49	2.064325e-46
MsG0580027826.01	MsG0680031621.01	0.833342	5.635020e-56	6.471596e-53
MsG0580027826.01	MsG0680031708.01	0.802981	4.333615e-49	2.152306e-46
MsG0580027826.01	MsG0680031939.01	0.835291	1.828070e-56	2.225879e-53
MsG0580027826.01	MsG0680032827.01	0.801444	8.978072e-49	4.288260e-46
MsG0580027826.01	MsG0680032830.01	0.820712	5.933453e-53	4.730643e-50
MsG0580027826.01	MsG0680032832.01	0.802115	6.538391e-49	3.176500e-46
MsG0580027826.01	MsG0680033377.01	0.834734	2.525374e-56	3.023132e-53
MsG0580027826.01	MsG0680033459.01	0.803568	3.276632e-49	1.652196e-46
MsG0580027826.01	MsG0680034064.01	0.821969	3.043691e-53	2.513559e-50
MsG0580027826.01	MsG0680034235.01	0.815910	7.258121e-52	5.068885e-49
MsG0580027826.01	MsG0680034767.01	0.803849	2.863852e-49	1.454499e-46
MsG0580027826.01	MsG0680035307.01	0.829798	4.206550e-55	4.351554e-52
MsG0580027826.01	MsG0680035859.01	0.805576	1.248636e-49	6.629349e-47
MsG0580027826.01	MsG0780036307.01	0.809311	2.014463e-50	1.178766e-47
MsG0580027826.01	MsG0780036585.01	0.801706	7.934635e-49	3.814912e-46
MsG0580027826.01	MsG0780036593.01	0.811842	5.718847e-51	3.577983e-48
MsG0580027826.01	MsG0780036659.01	0.830293	3.185699e-55	3.342294e-52
MsG0580027826.01	MsG0780036800.01	0.819239	1.288999e-52	9.864609e-50
MsG0580027826.01	MsG0780036979.01	0.829058	6.363396e-55	6.440000e-52
MsG0580027826.01	MsG0780037330.01	0.822882	1.867285e-53	1.582766e-50
MsG0580027826.01	MsG0780037341.01	0.836917	7.065392e-57	9.032855e-54
MsG0580027826.01	MsG0780037395.01	0.822133	2.788752e-53	2.313942e-50
MsG0580027826.01	MsG0780038784.01	0.811240	7.727445e-51	4.758407e-48
MsG0580027826.01	MsG0780038846.01	0.806519	7.906688e-50	4.300488e-47
MsG0580027826.01	MsG0780038847.01	0.804889	1.738840e-49	9.071627e-47
MsG0580027826.01	MsG0780038948.01	0.825633	4.214226e-54	3.862629e-51
MsG0580027826.01	MsG0780039186.01	0.809965	1.458004e-50	8.680586e-48
MsG0580027826.01	MsG0780039369.01	0.809775	1.601549e-50	9.488228e-48
MsG0580027826.01	MsG0780039439.01	0.818132	2.299696e-52	1.706899e-49
MsG0580027826.01	MsG0780039975.01	0.848473	5.992215e-60	1.101082e-56
MsG0580027826.01	MsG0780040278.01	0.834115	3.611263e-56	4.244303e-53
MsG0580027826.01	MsG0780040430.01	0.871283	7.691136e-67	3.085074e-63
MsG0580027826.01	MsG0780040431.01	0.880362	5.841769e-70	3.282117e-66
MsG0580027826.01	MsG0780041091.01	0.808398	3.157888e-50	1.803788e-47
MsG0580027826.01	MsG0780041259.01	0.806592	7.633158e-50	4.159294e-47
MsG0580027826.01	MsG0780041391.01	0.800846	1.189868e-48	5.598174e-46
MsG0580027826.01	MsG0780041440.01	0.813800	2.131059e-51	1.405391e-48
MsG0580027826.01	MsG0780041634.01	0.808393	3.166144e-50	1.808259e-47
MsG0580027826.01	MsG0780041673.01	0.809886	1.515678e-50	9.005068e-48
MsG0580027826.01	MsG0880041834.01	0.818310	2.095177e-52	1.562801e-49
MsG0580027826.01	MsG0880041947.01	0.803811	2.917128e-49	1.480048e-46
MsG0580027826.01	MsG0880044005.01	0.816187	6.292699e-52	4.428451e-49
MsG0580027826.01	MsG0880044036.01	0.804005	2.657863e-49	1.355291e-46
MsG0580027826.01	MsG0880044392.01	0.806652	7.411907e-50	4.044807e-47
MsG0580027826.01	MsG0880044487.01	0.830614	2.659743e-55	2.816761e-52
MsG0580027826.01	MsG0880044538.01	0.802399	5.715928e-49	2.797242e-46
MsG0580027826.01	MsG0880044704.01	0.820936	5.269853e-53	4.228084e-50
MsG0580027826.01	MsG0880045044.01	0.800065	1.716714e-48	7.918137e-46
MsG0580027826.01	MsG0880045223.01	0.803221	3.865424e-49	1.931671e-46
MsG0580027826.01	MsG0880045558.01	0.836181	1.087583e-56	1.360282e-53
MsG0580027826.01	MsG0880045668.01	0.827604	1.427526e-54	1.384659e-51
MsG0580027826.01	MsG0880045765.01	0.809575	1.768503e-50	1.042164e-47
MsG0580027826.01	MsG0880046314.01	0.805452	1.325639e-49	7.016034e-47
MsG0580027826.01	MsG0880046382.01	0.810418	1.163943e-50	7.012210e-48
MsG0580027826.01	MsG0880046583.01	0.800268	1.560780e-48	7.236198e-46
MsG0580027826.01	MsG0880046724.01	0.805353	1.390696e-49	7.341478e-47
MsG0580027826.01	MsG0880046736.01	0.824162	9.369869e-54	8.234626e-51
MsG0580027826.01	MsG0880046765.01	0.818714	1.697391e-52	1.280233e-49
MsG0580027826.01	MsG0880047135.01	0.844799	6.042967e-59	9.868139e-56
MsG0580027826.01	MsG0880047394.01	0.846298	2.371468e-59	4.063048e-56
MsG0580027826.01	MsG0880047538.01	0.848812	4.827877e-60	8.968458e-57
MsG0580027826.01	MsG0880047696.01	0.826840	2.174854e-54	2.063207e-51
MsG0580027826.01	MsG0880047703.01	0.805138	1.542822e-49	8.099783e-47
MsG0580027826.01	MsG0880047754.01	0.811367	7.252290e-51	4.480764e-48
MsG0280006592.01	MsG0580027826.01	0.833076	6.562293e-56	7.476738e-53
MsG0280006937.01	MsG0580027826.01	0.861439	1.025401e-63	2.901894e-60
MsG0280006996.01	MsG0580027826.01	0.816086	6.627756e-52	4.651514e-49
MsG0280007112.01	MsG0580027826.01	0.827616	1.418034e-54	1.375903e-51
MsG0280007118.01	MsG0580027826.01	0.807081	6.015049e-50	3.319280e-47
MsG0280007575.01	MsG0580027826.01	0.834470	2.942743e-56	3.495724e-53
MsG0280007976.01	MsG0580027826.01	0.834642	2.664029e-56	3.180671e-53
MsG0280008390.01	MsG0580027826.01	0.810805	9.601355e-51	5.844583e-48
MsG0280008392.01	MsG0580027826.01	0.804135	2.497901e-49	1.277934e-46
MsG0280008418.01	MsG0580027826.01	0.815334	9.748262e-52	6.701890e-49
MsG0280008709.01	MsG0580027826.01	0.806381	8.456755e-50	4.583347e-47
MsG0280008899.01	MsG0580027826.01	0.808608	2.849204e-50	1.636260e-47
MsG0280008929.01	MsG0580027826.01	0.802894	4.517454e-49	2.238756e-46
MsG0280008942.01	MsG0580027826.01	0.802301	5.985959e-49	2.922174e-46
MsG0280009256.01	MsG0580027826.01	0.821641	3.624304e-53	2.965358e-50
MsG0280009685.01	MsG0580027826.01	0.814778	1.295269e-51	8.771105e-49
MsG0280009756.01	MsG0580027826.01	0.800375	1.484327e-48	6.900645e-46
MsG0280010184.01	MsG0580027826.01	0.806874	6.653086e-50	3.651814e-47
MsG0280010503.01	MsG0580027826.01	0.823740	1.176982e-53	1.022249e-50

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027826.01.T01	MTR_5g063720	95.775	213	6	2	1	210	1	213	3.37e-146	405
MsG0580027826.01.T01	MTR_3g074450	56.140	228	70	11	1	209	1	217	1.10e-58	184

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027826.01.T01	AT1G78170	37.339	233	94	10	1	202	1	212	1.41e-26	102
MsG0580027826.01.T01	AT1G22250	42.609	115	52	3	109	209	85	199	4.75e-16	73.6
MsG0580027826.01.T01	AT4G08910	41.071	112	43	2	106	194	86	197	8.08e-16	73.2

Find 35 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACCACTTTCTGAATTCTTTA+AGG	0.050885	5:+69206146	MsG0580027826.01.T01:CDS
AATCACATAGACTTATTTAT+TGG	0.127321	5:-69206925	None:intergenic
CACCTTTGCCTTCAGATAAA+TGG	0.203598	5:+69206229	MsG0580027826.01.T01:CDS
CTTCACAAAAGAAGAGAAAA+TGG	0.266395	5:+69206115	MsG0580027826.01.T01:CDS
GCGAAGTTCTATATCAAATA+TGG	0.295622	5:-69206199	None:intergenic
AAACCACTCTTGTTTGAATA+TGG	0.302002	5:+69206032	MsG0580027826.01.T01:exon
ATCAATTAGCAAGAATAAAA+AGG	0.319819	5:+69206677	MsG0580027826.01.T01:CDS
CAAGAACAACCACTTCTAAT+TGG	0.345178	5:+69206901	MsG0580027826.01.T01:three_prime_UTR
AAGGATCTTACACATGATCT+AGG	0.356603	5:+69206165	MsG0580027826.01.T01:CDS
GGATGCATGAATTTGCAATT+AGG	0.374086	5:-69206816	None:intergenic
TCATTATATATTAAAACAAA+AGG	0.380890	5:+69206970	MsG0580027826.01.T01:three_prime_UTR
GTCAAATTACAACTCCTTAG+AGG	0.386148	5:-69207014	None:intergenic
TGGTTGTTCTTGATTATGTC+AGG	0.431587	5:-69206890	None:intergenic
TTAATCTACCTCTACCTCTA+AGG	0.434008	5:+69207000	MsG0580027826.01.T01:three_prime_UTR
GCAACAATACCTCACAGTTC+AGG	0.455526	5:+69206251	MsG0580027826.01.T01:CDS
AATTTGGAAGGGTTCTGATC+TGG	0.466878	5:-69206843	None:intergenic
GAAGGGTTCTGATCTGGTGG+TGG	0.491999	5:-69206837	None:intergenic
CTAAGGAGTTGTAATTTGAC+TGG	0.508158	5:+69207017	MsG0580027826.01.T01:three_prime_UTR
TTCACAAAAGAAGAGAAAAT+GGG	0.522716	5:+69206116	MsG0580027826.01.T01:CDS
CATTATATATTAAAACAAAA+GGG	0.524638	5:+69206971	MsG0580027826.01.T01:three_prime_UTR
TTACAACTCCTTAGAGGTAG+AGG	0.525637	5:-69207008	None:intergenic
CACATGTCAAATTAAGCTCA+AGG	0.527855	5:-69206596	None:intergenic
TATTGTTGCCATTTATCTGA+AGG	0.533510	5:-69206237	None:intergenic
AGGATCTTACACATGATCTA+GGG	0.553583	5:+69206166	MsG0580027826.01.T01:CDS
TCCTTAAAGAATTCAGAAAG+TGG	0.554882	5:-69206147	None:intergenic
TGTGAAGCATCAAAATCTGT+AGG	0.557840	5:-69206099	None:intergenic
AATTTGCAATTAGGACAAGA+AGG	0.564845	5:-69206807	None:intergenic
TTTATTGGACCAATTAGAAG+TGG	0.571145	5:-69206910	None:intergenic
TTGGAAGGGTTCTGATCTGG+TGG	0.597114	5:-69206840	None:intergenic
TGCCATTTATCTGAAGGCAA+AGG	0.597180	5:-69206231	None:intergenic
TGGTTATCATCATCATCAGA+TGG	0.614533	5:+69206711	MsG0580027826.01.T01:CDS
AGACCATATTCAAACAAGAG+TGG	0.639805	5:-69206035	None:intergenic
TTGTATGAAGTGTCACATGT+TGG	0.683627	5:+69206764	MsG0580027826.01.T01:CDS
TATTATATACCTGAACTGTG+AGG	0.723748	5:-69206260	None:intergenic
TATGATGATTATGATACACA+AGG	0.728153	5:-69207073	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CATTATATATTAAAACAAAA+GGG	+	Chr5:69206971-69206990	MsG0580027826.01.T01:three_prime_UTR	10.0%
!!	TCATTATATATTAAAACAAA+AGG	+	Chr5:69206970-69206989	MsG0580027826.01.T01:three_prime_UTR	10.0%
!!	AAAATGAAATATATTAACCT+CGG	-	Chr5:69206339-69206358	None:intergenic	15.0%
!!	AATCACATAGACTTATTTAT+TGG	-	Chr5:69206928-69206947	None:intergenic	20.0%
!!	ATAAAATATTATTGCTACCT+CGG	-	Chr5:69206385-69206404	None:intergenic	20.0%
!!	ATCAATTAGCAAGAATAAAA+AGG	+	Chr5:69206677-69206696	MsG0580027826.01.T01:CDS	20.0%
!	ATAAAAAGGATTCTTCATCA+TGG	+	Chr5:69206691-69206710	MsG0580027826.01.T01:CDS	25.0%
!	CGAAAATGTGCTTATAAAAA+GGG	-	Chr5:69206295-69206314	None:intergenic	25.0%
!	TATGATGATTATGATACACA+AGG	-	Chr5:69207076-69207095	None:intergenic	25.0%
!	TTCACAAAAGAAGAGAAAAT+GGG	+	Chr5:69206116-69206135	MsG0580027826.01.T01:CDS	25.0%
!	TTCTCTTCAAAAATTTGGAA+GGG	-	Chr5:69206857-69206876	None:intergenic	25.0%
	AATGTGCTTATAAAAAGGGA+CGG	-	Chr5:69206291-69206310	None:intergenic	30.0%
	AATTTGCAATTAGGACAAGA+AGG	-	Chr5:69206810-69206829	None:intergenic	30.0%
	ACCACTTTCTGAATTCTTTA+AGG	+	Chr5:69206146-69206165	MsG0580027826.01.T01:CDS	30.0%
	CCTTGTTCTCTTCAAAAATT+TGG	-	Chr5:69206862-69206881	None:intergenic	30.0%
	CTTCACAAAAGAAGAGAAAA+TGG	+	Chr5:69206115-69206134	MsG0580027826.01.T01:CDS	30.0%
	GCGAAAATGTGCTTATAAAA+AGG	-	Chr5:69206296-69206315	None:intergenic	30.0%
	GCGAAGTTCTATATCAAATA+TGG	-	Chr5:69206202-69206221	None:intergenic	30.0%
	GTTCTCTTCAAAAATTTGGA+AGG	-	Chr5:69206858-69206877	None:intergenic	30.0%
	TATTATATACCTGAACTGTG+AGG	-	Chr5:69206263-69206282	None:intergenic	30.0%
	TCCTTAAAGAATTCAGAAAG+TGG	-	Chr5:69206150-69206169	None:intergenic	30.0%
	TTACCTGAAAAATCATCTCT+AGG	+	Chr5:69206558-69206577	MsG0580027826.01.T01:CDS	30.0%
	TTTATTGGACCAATTAGAAG+TGG	-	Chr5:69206913-69206932	None:intergenic	30.0%
!	AAACCACTCTTGTTTGAATA+TGG	+	Chr5:69206032-69206051	MsG0580027826.01.T01:exon	30.0%
!	TAAATTTTCTCATGCAGTCA+GGG	+	Chr5:69206472-69206491	MsG0580027826.01.T01:intron	30.0%
!	TATTGTTGCCATTTATCTGA+AGG	-	Chr5:69206240-69206259	None:intergenic	30.0%
!	TTAAATTTTCTCATGCAGTC+AGG	+	Chr5:69206471-69206490	MsG0580027826.01.T01:intron	30.0%
!!	CCAAATTTTTGAAGAGAACA+AGG	+	Chr5:69206859-69206878	MsG0580027826.01.T01:CDS	30.0%
!!!	AGAGATGATTTTTCAGGTAA+AGG	-	Chr5:69206558-69206577	None:intergenic	30.0%
!!!	AGATTCTTTTTTTGGACTCA+TGG	-	Chr5:69206079-69206098	None:intergenic	30.0%
!!!	GAGATGATTTTTCAGGTAAA+GGG	-	Chr5:69206557-69206576	None:intergenic	30.0%
!!!	TCTGTAGGAGATTCTTTTTT+TGG	-	Chr5:69206087-69206106	None:intergenic	30.0%
	AAGGATCTTACACATGATCT+AGG	+	Chr5:69206165-69206184	MsG0580027826.01.T01:CDS	35.0%
	AGACCATATTCAAACAAGAG+TGG	-	Chr5:69206038-69206057	None:intergenic	35.0%
	AGGATCTTACACATGATCTA+GGG	+	Chr5:69206166-69206185	MsG0580027826.01.T01:CDS	35.0%
	CAAGAACAACCACTTCTAAT+TGG	+	Chr5:69206901-69206920	MsG0580027826.01.T01:three_prime_UTR	35.0%
	CACATGTCAAATTAAGCTCA+AGG	-	Chr5:69206599-69206618	None:intergenic	35.0%
	CTAAGGAGTTGTAATTTGAC+TGG	+	Chr5:69207017-69207036	MsG0580027826.01.T01:three_prime_UTR	35.0%
	GGATGCATGAATTTGCAATT+AGG	-	Chr5:69206819-69206838	None:intergenic	35.0%
	GTCAAATTACAACTCCTTAG+AGG	-	Chr5:69207017-69207036	None:intergenic	35.0%
	TGGTTATCATCATCATCAGA+TGG	+	Chr5:69206711-69206730	MsG0580027826.01.T01:CDS	35.0%
	TGGTTGTTCTTGATTATGTC+AGG	-	Chr5:69206893-69206912	None:intergenic	35.0%
	TTAATCTACCTCTACCTCTA+AGG	+	Chr5:69207000-69207019	MsG0580027826.01.T01:three_prime_UTR	35.0%
	TTCAAAACAAATTAGAGCCG+AGG	+	Chr5:69206365-69206384	MsG0580027826.01.T01:intron	35.0%
!	TGACCTAGAGATGATTTTTC+AGG	-	Chr5:69206564-69206583	None:intergenic	35.0%
!	TGTGAAGCATCAAAATCTGT+AGG	-	Chr5:69206102-69206121	None:intergenic	35.0%
!	TTGTATGAAGTGTCACATGT+TGG	+	Chr5:69206764-69206783	MsG0580027826.01.T01:CDS	35.0%
!!	CTAGTTTTTAATTAGAGCCG+AGG	+	Chr5:69206319-69206338	MsG0580027826.01.T01:intron	35.0%
	CACCTTTGCCTTCAGATAAA+TGG	+	Chr5:69206229-69206248	MsG0580027826.01.T01:CDS	40.0%
	TTACAACTCCTTAGAGGTAG+AGG	-	Chr5:69207011-69207030	None:intergenic	40.0%
!	AATTTGGAAGGGTTCTGATC+TGG	-	Chr5:69206846-69206865	None:intergenic	40.0%
!	TGCCATTTATCTGAAGGCAA+AGG	-	Chr5:69206234-69206253	None:intergenic	40.0%
	GCAACAATACCTCACAGTTC+AGG	+	Chr5:69206251-69206270	MsG0580027826.01.T01:CDS	45.0%
	TTCTCTCCAGTAGTTGTGAG+CGG	-	Chr5:69205998-69206017	None:intergenic	45.0%
!!!	CTGAAGGCAAAGGTGTTTTG+AGG	-	Chr5:69206224-69206243	None:intergenic	45.0%
!	TTGGAAGGGTTCTGATCTGG+TGG	-	Chr5:69206843-69206862	None:intergenic	50.0%
!	GAAGGGTTCTGATCTGGTGG+TGG	-	Chr5:69206840-69206859	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	69205997	69207131	69205997	ID=MsG0580027826.01;Name=MsG0580027826.01
Chr5	mRNA	69205997	69207131	69205997	ID=MsG0580027826.01.T01;Parent=MsG0580027826.01;Name=MsG0580027826.01.T01;_AED=0.14;_eAED=0.14;_QI=54\|1\|1\|1\|1\|1\|2\|232\|210
Chr5	exon	69205997	69206272	69205997	ID=MsG0580027826.01.T01:exon:7017;Parent=MsG0580027826.01.T01
Chr5	exon	69206489	69207131	69206489	ID=MsG0580027826.01.T01:exon:7018;Parent=MsG0580027826.01.T01
Chr5	five_prime_UTR	69205997	69206050	69205997	ID=MsG0580027826.01.T01:five_prime_utr;Parent=MsG0580027826.01.T01
Chr5	CDS	69206051	69206272	69206051	ID=MsG0580027826.01.T01:cds;Parent=MsG0580027826.01.T01
Chr5	CDS	69206489	69206899	69206489	ID=MsG0580027826.01.T01:cds;Parent=MsG0580027826.01.T01
Chr5	three_prime_UTR	69206900	69207131	69206900	ID=MsG0580027826.01.T01:three_prime_utr;Parent=MsG0580027826.01.T01

Gene Sequence

>MsG0580027826.01.T01

ATGGTCTCACTTCACAAAGCACTAGCCATGAGTCCAAAAAAAGAATCTCCTACAGATTTTGATGCTTCACAAAAGAAGAGAAAATGGGAAGAATCACCACTTTCTGAATTCTTTAAGGATCTTACACATGATCTAGGGAAAAGAAAATCCATATTTGATATAGAACTTCGCCTCAAAACACCTTTGCCTTCAGATAAATGGCAACAATACCTCACAGTTCAGTCAGGGCAAATACAATTGTGTAACACAAAGATGAATATGAAAACACAAGAATCAAAAAGAGAGTACCCTTTACCTGAAAAATCATCTCTAGGTCATTTAAGCTTAGACCTTGAGCTTAATTTGACATGTGAAACATTGAAGAAAAAAGAAGAAAGTTATTTTGAAAAGAAGAATAATCTAGTGAATGAATCAATTAGCAAGAATAAAAAGGATTCTTCATCATGGTTATCATCATCATCAGATGGTGATGATTACAAAGAAATGATTGCAACAGTTTGTATGAAGTGTCACATGTTGGTAATGCTTTGCAAATCATCTCCTTCTTGTCCTAATTGCAAATTCATGCATCCACCACCAGATCAGAACCCTTCCAAATTTTTGAAGAGAACAAGGTTTAGTTTCCTGACATAA

Protein sequence

>MsG0580027826.01.T01

MVSLHKALAMSPKKESPTDFDASQKKRKWEESPLSEFFKDLTHDLGKRKSIFDIELRLKTPLPSDKWQQYLTVQSGQIQLCNTKMNMKTQESKREYPLPEKSSLGHLSLDLELNLTCETLKKKEESYFEKKNNLVNESISKNKKDSSSWLSSSSDGDDYKEMIATVCMKCHMLVMLCKSSPSCPNCKFMHPPPDQNPSKFLKRTRFSFLT*