AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043028.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043028.01.T01 MTR_8g027805 82.418 91 4 1 1 79 68 158 1.53e-45 144
MsG0880043028.01.T01 MTR_3g090980 68.132 91 17 1 1 79 71 161 2.85e-37 124
MsG0880043028.01.T01 MTR_1g028380 64.835 91 20 1 1 79 69 159 3.51e-36 120
MsG0880043028.01.T01 MTR_3g071740 51.136 88 28 3 4 79 121 205 7.29e-20 80.1
MsG0880043028.01.T01 MTR_5g083270 50.000 88 28 3 3 77 100 184 6.06e-19 77.0
MsG0880043028.01.T01 MTR_1g016480 47.191 89 34 2 1 76 89 177 5.03e-18 74.7
MsG0880043028.01.T01 MTR_4g014460 44.186 86 36 1 3 76 102 187 1.48e-16 70.9
MsG0880043028.01.T01 MTR_5g030500 47.727 88 29 3 5 76 100 186 5.73e-15 66.6
MsG0880043028.01.T01 MTR_7g070050 50.000 94 30 3 3 79 66 159 2.03e-13 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043028.01.T01 AT1G01360 71.910 89 13 1 3 79 73 161 1.06e-37 124
MsG0880043028.01.T01 AT4G01026 69.231 91 16 1 1 79 73 163 1.60e-37 124
MsG0880043028.01.T01 AT5G53160 64.835 91 20 1 1 79 69 159 1.91e-34 116
MsG0880043028.01.T01 AT4G27920 58.696 92 25 2 1 79 64 155 1.13e-28 101
MsG0880043028.01.T01 AT2G40330 50.588 85 28 2 7 77 106 190 7.22e-18 74.3
MsG0880043028.01.T01 AT2G26040 46.154 91 33 3 1 76 73 162 1.04e-15 68.2
MsG0880043028.01.T01 AT4G17870 45.882 85 34 1 4 76 72 156 1.76e-15 67.8
MsG0880043028.01.T01 AT5G05440 47.191 89 31 3 1 76 96 181 2.48e-15 67.4
MsG0880043028.01.T01 AT2G38310 51.190 84 26 2 6 77 96 176 5.52e-13 61.6
MsG0880043028.01.T01 AT1G73000 38.889 90 38 2 4 76 96 185 1.75e-12 60.1

Find 25 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCCGGATGGACTGTGATTA+TGG 0.268603 8:-17895879 None:intergenic
TCGTCCTTCGTGTTCCCTTC+AGG 0.331294 8:-17895956 None:intergenic
TGATGATGAGGACATATTCT+TGG 0.357998 8:+17895337 MsG0880043028.01.T01:CDS
CTCTTACACTTCCAATACTA+AGG 0.431766 8:-17895256 None:intergenic
AGAGAAGTGAATGTTAAATC+TGG 0.439454 8:+17895275 MsG0880043028.01.T01:CDS
CTCTCAGTACTTGTTGTAGC+TGG 0.467121 8:-17895302 None:intergenic
TTCCATAATCACAGTCCATC+CGG 0.473119 8:+17895877 MsG0880043028.01.T01:CDS
ATGCAAGGTGACCTTAGTAT+TGG 0.474026 8:+17895245 MsG0880043028.01.T01:CDS
GTCCATCCGGAGGTTATTGA+CGG 0.527187 8:+17895890 MsG0880043028.01.T01:CDS
GAGGTTATTGACGGAAGACC+AGG 0.540051 8:+17895899 MsG0880043028.01.T01:CDS
TACAACAAGTACTGAGAGGT+TGG 0.543951 8:+17895307 MsG0880043028.01.T01:CDS
AGGACGAAACTTGTTACTTG+TGG 0.552324 8:+17895972 MsG0880043028.01.T01:CDS
GATTCAATCACCATTGTACC+TGG 0.555628 8:-17895917 None:intergenic
TTCGTCGTGGACGTACCTGA+AGG 0.560275 8:+17895941 MsG0880043028.01.T01:CDS
GGTCTTCCGTCAATAACCTC+CGG 0.572740 8:-17895896 None:intergenic
ACGAAACTTGTTACTTGTGG+AGG 0.583274 8:+17895975 MsG0880043028.01.T01:CDS
TTCCGTCAATAACCTCCGGA+TGG 0.584863 8:-17895892 None:intergenic
ATTAGCAGGTGTATTATGCA+AGG 0.592791 8:+17895230 None:intergenic
TCGTCGTGGACGTACCTGAA+GGG 0.600827 8:+17895942 MsG0880043028.01.T01:CDS
GTTGGAACAACTTGATGATG+AGG 0.610978 8:+17895325 MsG0880043028.01.T01:CDS
TGACGGAAGACCAGGTACAA+TGG 0.649685 8:+17895907 MsG0880043028.01.T01:CDS
GGTGATTGAATCATTCGTCG+TGG 0.681706 8:+17895928 MsG0880043028.01.T01:CDS
CAGCTACAACAAGTACTGAG+AGG 0.700141 8:+17895303 MsG0880043028.01.T01:CDS
CATAATCACAGTCCATCCGG+AGG 0.702023 8:+17895880 MsG0880043028.01.T01:CDS
CGTACCTGAAGGGAACACGA+AGG 0.780385 8:+17895952 MsG0880043028.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAGAAAAATATTTTGAATT+TGG - Chr8:17895425-17895444 None:intergenic 10.0%
!!! TTTTTACTTATTAATAGAAA+TGG + Chr8:17895721-17895740 MsG0880043028.01.T01:intron 10.0%
!! AAATGAAGAAATTGTATATA+TGG + Chr8:17895481-17895500 MsG0880043028.01.T01:intron 15.0%
!!! TTTTCCAAAAAAAAAATCAA+TGG + Chr8:17895615-17895634 MsG0880043028.01.T01:intron 15.0%
!! CAAAATACCAAACTTAAAAT+TGG - Chr8:17895593-17895612 None:intergenic 20.0%
!!! CAATTTTAAGTTTGGTATTT+TGG + Chr8:17895591-17895610 MsG0880043028.01.T01:intron 20.0%
!!! TTTTGAATTTGGAAAAACAA+AGG - Chr8:17895414-17895433 None:intergenic 20.0%
! GAGAGATTATCTTATAATCT+TGG + Chr8:17895664-17895683 MsG0880043028.01.T01:intron 25.0%
! TCTTATGTTTATGTCTTCAT+AGG + Chr8:17895785-17895804 MsG0880043028.01.T01:intron 25.0%
! TTACTATGATTGAAATTCAG+CGG + Chr8:17895529-17895548 MsG0880043028.01.T01:intron 25.0%
!!! GTGACCATTGATTTTTTTTT+TGG - Chr8:17895622-17895641 None:intergenic 25.0%
AAAAAATCAATGGTCACCAA+TGG + Chr8:17895625-17895644 MsG0880043028.01.T01:intron 30.0%
AGAGAAGTGAATGTTAAATC+TGG + Chr8:17895275-17895294 MsG0880043028.01.T01:CDS 30.0%
ATTCTTGGTATCAGGATTGT+TGG + Chr8:17895352-17895371 MsG0880043028.01.T01:intron 35.0%
CTCTTACACTTCCAATACTA+AGG - Chr8:17895259-17895278 None:intergenic 35.0%
! TGATGATGAGGACATATTCT+TGG + Chr8:17895337-17895356 MsG0880043028.01.T01:CDS 35.0%
!!! AGACGGTCCAATTTTAAGTT+TGG + Chr8:17895583-17895602 MsG0880043028.01.T01:intron 35.0%
AAAATTGGACCGTCTGATCT+GGG - Chr8:17895578-17895597 None:intergenic 40.0%
AGCATCAGTTGTAAGCTGAA+CGG + Chr8:17895815-17895834 MsG0880043028.01.T01:intron 40.0%
ATCGTTAAACCCAGATCAGA+CGG + Chr8:17895566-17895585 MsG0880043028.01.T01:intron 40.0%
ATGCAAGGTGACCTTAGTAT+TGG + Chr8:17895245-17895264 MsG0880043028.01.T01:CDS 40.0%
CATTCACACATTCAGTCCAT+TGG - Chr8:17895644-17895663 None:intergenic 40.0%
GAGGACATATTCTTGGTATC+AGG + Chr8:17895344-17895363 MsG0880043028.01.T01:intron 40.0%
GATTCAATCACCATTGTACC+TGG - Chr8:17895920-17895939 None:intergenic 40.0%
TAAAATTGGACCGTCTGATC+TGG - Chr8:17895579-17895598 None:intergenic 40.0%
TTCCATAATCACAGTCCATC+CGG + Chr8:17895877-17895896 MsG0880043028.01.T01:CDS 40.0%
!! ACGAAACTTGTTACTTGTGG+AGG + Chr8:17895975-17895994 MsG0880043028.01.T01:CDS 40.0%
!! AGGACGAAACTTGTTACTTG+TGG + Chr8:17895972-17895991 MsG0880043028.01.T01:CDS 40.0%
!! CAATGGACTGAATGTGTGAA+TGG + Chr8:17895642-17895661 MsG0880043028.01.T01:intron 40.0%
!! GTTGGAACAACTTGATGATG+AGG + Chr8:17895325-17895344 MsG0880043028.01.T01:CDS 40.0%
!! TACAACAAGTACTGAGAGGT+TGG + Chr8:17895307-17895326 MsG0880043028.01.T01:CDS 40.0%
AAATTCAGCGGTCAGCACAT+CGG + Chr8:17895541-17895560 MsG0880043028.01.T01:intron 45.0%
CTTGGTATCAGGATTGTTGG+TGG + Chr8:17895355-17895374 MsG0880043028.01.T01:intron 45.0%
GGTGATTGAATCATTCGTCG+TGG + Chr8:17895928-17895947 MsG0880043028.01.T01:CDS 45.0%
! TGGTGGTGATCATAGACTGA+GGG + Chr8:17895372-17895391 MsG0880043028.01.T01:intron 45.0%
!! CAGCTACAACAAGTACTGAG+AGG + Chr8:17895303-17895322 MsG0880043028.01.T01:CDS 45.0%
!! CTCTCAGTACTTGTTGTAGC+TGG - Chr8:17895305-17895324 None:intergenic 45.0%
!! TTGGTGGTGATCATAGACTG+AGG + Chr8:17895371-17895390 MsG0880043028.01.T01:intron 45.0%
!! TATTAATAAGTAAAAAAATA+TGG - Chr8:17895717-17895736 None:intergenic 5.0%
CATAATCACAGTCCATCCGG+AGG + Chr8:17895880-17895899 MsG0880043028.01.T01:CDS 50.0%
GAGGTTATTGACGGAAGACC+AGG + Chr8:17895899-17895918 MsG0880043028.01.T01:CDS 50.0%
GGTCTTCCGTCAATAACCTC+CGG - Chr8:17895899-17895918 None:intergenic 50.0%
GTCCATCCGGAGGTTATTGA+CGG + Chr8:17895890-17895909 MsG0880043028.01.T01:CDS 50.0%
TGACGGAAGACCAGGTACAA+TGG + Chr8:17895907-17895926 MsG0880043028.01.T01:CDS 50.0%
TTCCGTCAATAACCTCCGGA+TGG - Chr8:17895895-17895914 None:intergenic 50.0%
!! CTCCGGATGGACTGTGATTA+TGG - Chr8:17895882-17895901 None:intergenic 50.0%
CGTACCTGAAGGGAACACGA+AGG + Chr8:17895952-17895971 MsG0880043028.01.T01:CDS 55.0%
TCGTCCTTCGTGTTCCCTTC+AGG - Chr8:17895959-17895978 None:intergenic 55.0%
TCGTCGTGGACGTACCTGAA+GGG + Chr8:17895942-17895961 MsG0880043028.01.T01:CDS 55.0%
TTCGTCGTGGACGTACCTGA+AGG + Chr8:17895941-17895960 MsG0880043028.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 17895245 17896003 17895245 ID=MsG0880043028.01;Name=MsG0880043028.01
Chr8 mRNA 17895245 17896003 17895245 ID=MsG0880043028.01.T01;Parent=MsG0880043028.01;Name=MsG0880043028.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|82
Chr8 exon 17895245 17895358 17895245 ID=MsG0880043028.01.T01:exon:3531;Parent=MsG0880043028.01.T01
Chr8 exon 17895869 17896003 17895869 ID=MsG0880043028.01.T01:exon:3532;Parent=MsG0880043028.01.T01
Chr8 CDS 17895245 17895358 17895245 ID=MsG0880043028.01.T01:cds;Parent=MsG0880043028.01.T01
Chr8 CDS 17895869 17896003 17895869 ID=MsG0880043028.01.T01:cds;Parent=MsG0880043028.01.T01
Gene Sequence

>MsG0880043028.01.T01

ATGCAAGGTGACCTTAGTATTGGAAGTGTAAGAGAAGTGAATGTTAAATCTGGTCTTCCAGCTACAACAAGTACTGAGAGGTTGGAACAACTTGATGATGAGGACATATTCTTGAACTATTCTTCCATAATCACAGTCCATCCGGAGGTTATTGACGGAAGACCAGGTACAATGGTGATTGAATCATTCGTCGTGGACGTACCTGAAGGGAACACGAAGGACGAAACTTGTTACTTGTGGAGGCGTTGA

Protein sequence

>MsG0880043028.01.T01

MQGDLSIGSVREVNVKSGLPATTSTERLEQLDDEDIFLNYSSIITVHPEVIDGRPGTMVIESFVVDVPEGNTKDETCYLWRR*