AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780036810.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036810.01.T01 MTR_0003s0690 94.220 173 10 0 59 231 1 173 6.91e-122 343
MsG0780036810.01.T01 MTR_7g109780 73.057 193 47 2 44 231 1 193 8.79e-102 293
MsG0780036810.01.T01 MTR_7g109760 42.771 166 38 5 1 164 1 111 9.10e-32 112
MsG0780036810.01.T03 MTR_0003s0690 94.220 173 10 0 1 173 1 173 4.23e-121 339
MsG0780036810.01.T03 MTR_7g109780 74.157 178 41 2 1 173 16 193 3.43e-94 271
MsG0780036810.01.T03 MTR_7g109760 49.533 107 26 3 1 106 32 111 2.65e-26 97.1
MsG0780036810.01.T02 MTR_0003s0690 94.220 173 10 0 16 188 1 173 1.30e-121 341
MsG0780036810.01.T02 MTR_7g109780 73.057 193 47 2 1 188 1 193 1.23e-101 291
MsG0780036810.01.T02 MTR_7g109760 49.533 107 26 3 16 121 32 111 3.73e-26 97.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036810.01.T01 AT5G23710 37.209 215 128 3 21 231 19 230 2.57e-47 156
MsG0780036810.01.T03 AT5G23710 39.548 177 101 2 1 173 56 230 2.31e-41 139
MsG0780036810.01.T02 AT5G23710 38.021 192 113 2 1 188 41 230 3.23e-44 147

Find 37 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGATGACAAGTACTGGATA+TGG 0.326445 7:-14304960 MsG0780036810.01.T01:CDS
ATGAAACGTTTGCAAGAATC+TGG 0.362818 7:-14305452 MsG0780036810.01.T01:CDS
GATGTTTGGAGTGGGTTAAA+AGG 0.362955 7:-14305064 MsG0780036810.01.T01:CDS
GGCACTTATAAACAAGAGTA+TGG 0.381632 7:-14305273 MsG0780036810.01.T01:CDS
GAGCCTTCCAAGGATATAAC+TGG 0.383383 7:-14305191 MsG0780036810.01.T01:CDS
TGAAAATAGACACATGTTGT+TGG 0.391859 7:+14304798 None:intergenic
TTCCACATGTGATGGATGTT+TGG 0.402495 7:-14305078 MsG0780036810.01.T01:CDS
GTGCTTGCAATGCATTTCAT+TGG 0.426796 7:-14305108 MsG0780036810.01.T01:CDS
TAGAGTATACGCTCTTCATC+TGG 0.446970 7:+14305392 None:intergenic
TGTTTATTAAGCGCAGCAAT+AGG 0.447212 7:+14305302 None:intergenic
ATGCCCACCAGTTATATCCT+TGG 0.459091 7:+14305184 None:intergenic
TGGAGTGGGTTAAAAGGAGT+GGG 0.480413 7:-14305058 MsG0780036810.01.T01:CDS
AGATGACAAGTACTGGATAT+GGG 0.485810 7:-14304959 MsG0780036810.01.T01:CDS
CAAAGGTATAAAGATCTACA+TGG 0.502338 7:-14305225 MsG0780036810.01.T01:CDS
ATAATGCAGATGACAAGTAC+TGG 0.506988 7:-14304966 MsG0780036810.01.T01:CDS
ATACTTAGCAAGGAAAACTC+AGG 0.536886 7:-14305362 MsG0780036810.01.T01:CDS
TATAAACAAGAGTATGGTAA+AGG 0.546896 7:-14305267 MsG0780036810.01.T01:CDS
TTGGAGTGGGTTAAAAGGAG+TGG 0.562912 7:-14305059 MsG0780036810.01.T01:CDS
AACAGAATTTGAGCCTTCCA+AGG 0.568058 7:-14305201 MsG0780036810.01.T01:CDS
TATTAAGCGCAGCAATAGGA+AGG 0.568531 7:+14305306 None:intergenic
GAGAGAGTTTCCACATGTGA+TGG 0.576485 7:-14305086 MsG0780036810.01.T01:CDS
TTGTTACATATGCAAAAGCA+AGG 0.588438 7:-14304907 MsG0780036810.01.T01:CDS
GTCATCACAACTCAAGTCAC+GGG 0.589333 7:-14305035 MsG0780036810.01.T01:CDS
TGCGCTTAATAAACACATGA+AGG 0.598707 7:-14305294 MsG0780036810.01.T01:CDS
CTATGACCTTATACTTAGCA+AGG 0.601401 7:-14305372 MsG0780036810.01.T01:CDS
CCACCAGTTATATCCTTGGA+AGG 0.602371 7:+14305188 None:intergenic
GGTCATCACAACTCAAGTCA+CGG 0.608686 7:-14305036 MsG0780036810.01.T01:CDS
CCTTCCAAGGATATAACTGG+TGG 0.618508 7:-14305188 MsG0780036810.01.T01:CDS
CTCCAAACATCCATCACATG+TGG 0.618959 7:+14305076 None:intergenic
TACTTAGCAAGGAAAACTCA+GGG 0.624253 7:-14305361 MsG0780036810.01.T01:CDS
GATGACAAGTACTGGATATG+GGG 0.631855 7:-14304958 MsG0780036810.01.T01:CDS
GGAGTGGGTTAAAAGGAGTG+GGG 0.639610 7:-14305057 MsG0780036810.01.T01:CDS
CATGTGATGGATGTTTGGAG+TGG 0.639686 7:-14305073 MsG0780036810.01.T01:CDS
ATGTGATGGATGTTTGGAGT+GGG 0.644389 7:-14305072 MsG0780036810.01.T01:CDS
TGTTACATATGCAAAAGCAA+GGG 0.651007 7:-14304906 MsG0780036810.01.T01:CDS
AAAGGTATAAAGATCTACAT+GGG 0.652631 7:-14305224 MsG0780036810.01.T01:CDS
CTTCCAAGGATATAACTGGT+GGG 0.673279 7:-14305187 MsG0780036810.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTATACCTTTGTTTTTAA+TGG + Chr7:14304999-14305018 None:intergenic 20.0%
!! AAAGGTATAAAGATCTACAT+GGG - Chr7:14305007-14305026 MsG0780036810.01.T01:CDS 25.0%
!! TATAAACAAGAGTATGGTAA+AGG - Chr7:14304964-14304983 MsG0780036810.01.T01:CDS 25.0%
!!! ATACCTTTGTTTTTAATGGA+AGG + Chr7:14304995-14305014 None:intergenic 25.0%
CTTCCTTCCATTAAAAACAA+AGG - Chr7:14304989-14305008 MsG0780036810.01.T01:CDS 30.0%
TGAAAATAGACACATGTTGT+TGG + Chr7:14305436-14305455 None:intergenic 30.0%
TGACAAGAAAAACATGGAAA+GGG + Chr7:14305376-14305395 None:intergenic 30.0%
TGTTACATATGCAAAAGCAA+GGG - Chr7:14305325-14305344 MsG0780036810.01.T01:CDS 30.0%
TTGACAAGAAAAACATGGAA+AGG + Chr7:14305377-14305396 None:intergenic 30.0%
TTGTTACATATGCAAAAGCA+AGG - Chr7:14305324-14305343 MsG0780036810.01.T01:CDS 30.0%
! AGAAGAGATTTTGCAAACTT+TGG - Chr7:14305228-14305247 MsG0780036810.01.T01:CDS 30.0%
! AGTTTTCCTTGCTAAGTATA+AGG + Chr7:14304868-14304887 None:intergenic 30.0%
! CAAAGGTATAAAGATCTACA+TGG - Chr7:14305006-14305025 MsG0780036810.01.T01:CDS 30.0%
! CATGTGTCTATTTTCAGAAA+TGG - Chr7:14305440-14305459 MsG0780036810.01.T01:CDS 30.0%
! TGTCTATTTTCAGAAATGGT+TGG - Chr7:14305444-14305463 MsG0780036810.01.T01:CDS 30.0%
! TTCCATGTTTTTCTTGTCAA+CGG - Chr7:14305377-14305396 MsG0780036810.01.T01:CDS 30.0%
ACAGAAACGACAAAAACTGT+CGG - Chr7:14304811-14304830 MsG0780036810.01.T01:CDS 35.0%
ATAATGCAGATGACAAGTAC+TGG - Chr7:14305265-14305284 MsG0780036810.01.T01:CDS 35.0%
ATACTTAGCAAGGAAAACTC+AGG - Chr7:14304869-14304888 MsG0780036810.01.T01:CDS 35.0%
ATCCGTTGACAAGAAAAACA+TGG + Chr7:14305382-14305401 None:intergenic 35.0%
ATGAAACGTTTGCAAGAATC+TGG - Chr7:14304779-14304798 MsG0780036810.01.T01:CDS 35.0%
CTATGACCTTATACTTAGCA+AGG - Chr7:14304859-14304878 MsG0780036810.01.T01:CDS 35.0%
GGCACTTATAAACAAGAGTA+TGG - Chr7:14304958-14304977 MsG0780036810.01.T01:CDS 35.0%
TACTTAGCAAGGAAAACTCA+GGG - Chr7:14304870-14304889 MsG0780036810.01.T01:CDS 35.0%
TGCGCTTAATAAACACATGA+AGG - Chr7:14304937-14304956 MsG0780036810.01.T01:CDS 35.0%
TGTTTATTAAGCGCAGCAAT+AGG + Chr7:14304932-14304951 None:intergenic 35.0%
! ATGTAACAAGTTTTGCCAAC+AGG + Chr7:14305313-14305332 None:intergenic 35.0%
! GATTTTGCATCTATTCCTGT+TGG - Chr7:14305295-14305314 MsG0780036810.01.T01:CDS 35.0%
!! AGATGACAAGTACTGGATAT+GGG - Chr7:14305272-14305291 MsG0780036810.01.T01:CDS 35.0%
!! TTTTTGTCGTTTCTGTTGAG+AGG + Chr7:14304807-14304826 None:intergenic 35.0%
AACAGAATTTGAGCCTTCCA+AGG - Chr7:14305030-14305049 MsG0780036810.01.T01:CDS 40.0%
CTTCCAAGGATATAACTGGT+GGG - Chr7:14305044-14305063 MsG0780036810.01.T01:CDS 40.0%
GTGCTTGCAATGCATTTCAT+TGG - Chr7:14305123-14305142 MsG0780036810.01.T01:CDS 40.0%
TAGAGTATACGCTCTTCATC+TGG + Chr7:14304842-14304861 None:intergenic 40.0%
TATTAAGCGCAGCAATAGGA+AGG + Chr7:14304928-14304947 None:intergenic 40.0%
! ACGGATGAGCTTTTGTTCAA+CGG - Chr7:14305396-14305415 MsG0780036810.01.T01:CDS 40.0%
! ATGAGCTTTTGTTCAACGGA+TGG - Chr7:14305400-14305419 MsG0780036810.01.T01:CDS 40.0%
!! ATGTGATGGATGTTTGGAGT+GGG - Chr7:14305159-14305178 MsG0780036810.01.T01:CDS 40.0%
!! CAGATGACAAGTACTGGATA+TGG - Chr7:14305271-14305290 MsG0780036810.01.T01:CDS 40.0%
!! GATGACAAGTACTGGATATG+GGG - Chr7:14305273-14305292 MsG0780036810.01.T01:CDS 40.0%
!! GATGTTTGGAGTGGGTTAAA+AGG - Chr7:14305167-14305186 MsG0780036810.01.T01:CDS 40.0%
!! TTCCACATGTGATGGATGTT+TGG - Chr7:14305153-14305172 MsG0780036810.01.T01:CDS 40.0%
ATGCCCACCAGTTATATCCT+TGG + Chr7:14305050-14305069 None:intergenic 45.0%
CCACCAGTTATATCCTTGGA+AGG + Chr7:14305046-14305065 None:intergenic 45.0%
CCTTCCAAGGATATAACTGG+TGG - Chr7:14305043-14305062 MsG0780036810.01.T01:CDS 45.0%
CTCCAAACATCCATCACATG+TGG + Chr7:14305158-14305177 None:intergenic 45.0%
GAGAGAGTTTCCACATGTGA+TGG - Chr7:14305145-14305164 MsG0780036810.01.T01:CDS 45.0%
GAGCCTTCCAAGGATATAAC+TGG - Chr7:14305040-14305059 MsG0780036810.01.T01:CDS 45.0%
GGTCATCACAACTCAAGTCA+CGG - Chr7:14305195-14305214 MsG0780036810.01.T01:CDS 45.0%
GTCATCACAACTCAAGTCAC+GGG - Chr7:14305196-14305215 MsG0780036810.01.T01:CDS 45.0%
! GTGGGCATTTTTACAGCGAA+GGG - Chr7:14305062-14305081 MsG0780036810.01.T01:CDS 45.0%
!! CATGTGATGGATGTTTGGAG+TGG - Chr7:14305158-14305177 MsG0780036810.01.T01:CDS 45.0%
!! TGGAGTGGGTTAAAAGGAGT+GGG - Chr7:14305173-14305192 MsG0780036810.01.T01:CDS 45.0%
!! TTGGAGTGGGTTAAAAGGAG+TGG - Chr7:14305172-14305191 MsG0780036810.01.T01:CDS 45.0%
! GGTGGGCATTTTTACAGCGA+AGG - Chr7:14305061-14305080 MsG0780036810.01.T01:CDS 50.0%
!! GGAGTGGGTTAAAAGGAGTG+GGG - Chr7:14305174-14305193 MsG0780036810.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr7 gene 14304779 14305474 14304779 ID=MsG0780036810.01;Name=MsG0780036810.01
Chr7 mRNA 14304779 14305474 14304779 ID=MsG0780036810.01.T01;Parent=MsG0780036810.01;Name=MsG0780036810.01.T01;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|231
Chr7 exon 14304779 14305474 14304779 ID=MsG0780036810.01.T01:exon:2206;Parent=MsG0780036810.01.T01
Chr7 CDS 14304779 14305474 14304779 ID=MsG0780036810.01.T01:cds;Parent=MsG0780036810.01.T01
Chr7 mRNA 14304779 14305345 14304779 ID=MsG0780036810.01.T02;Parent=MsG0780036810.01;Name=MsG0780036810.01.T02;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|0|1|0|188
Chr7 exon 14304779 14305345 14304779 ID=MsG0780036810.01.T02:exon:2207;Parent=MsG0780036810.01.T02
Chr7 CDS 14304779 14305345 14304779 ID=MsG0780036810.01.T02:cds;Parent=MsG0780036810.01.T02
Chr7 mRNA 14304779 14305300 14304779 ID=MsG0780036810.01.T03;Parent=MsG0780036810.01;Name=MsG0780036810.01.T03;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|0|1|0|173
Chr7 exon 14304779 14305300 14304779 ID=MsG0780036810.01.T03:exon:2208;Parent=MsG0780036810.01.T03
Chr7 CDS 14304779 14305300 14304779 ID=MsG0780036810.01.T03:cds;Parent=MsG0780036810.01.T03
Gene Sequence

>MsG0780036810.01.T01

ATGAAACGTTTGCAAGAATCTGGTTCCTCTCAACAGAAACGACAAAAACTGTCGGCGCTGCCAGATGAAGAGCGTATACTCTATGACCTTATACTTAGCAAGGAAAACTCAGGGATCTCAAAAACAGACATGAGAAGAGAAACAAACCTTCCTATTGCTGCGCTTAATAAACACATGAAGGCACTTATAAACAAGAGTATGGTAAAGGAACTTCCTTCCATTAAAAACAAAGGTATAAAGATCTACATGGGAACAGAATTTGAGCCTTCCAAGGATATAACTGGTGGGCATTTTTACAGCGAAGGGAGTCTTGATACGCTTTTTATAGACGCTCTAAAGACAGTGTGCTTGCAATGCATTTCATTGGAGAGAGTTTCCACATGTGATGGATGTTTGGAGTGGGTTAAAAGGAGTGGGGTCATCACAACTCAAGTCACGGGAGAACAAATAGAAGAGATTTTGCAAACTTTGGTTTTAGATGATGAGATAATGCAGATGACAAGTACTGGATATGGGGATTTTGCATCTATTCCTGTTGGCAAAACTTGTTACATATGCAAAAGCAAGGGTGAAAAGAAATCTGCTGACTCGACTCCCTTTCCATGTTTTTCTTGTCAACGGATGAGCTTTTGTTCAACGGATGGCGTTGTCTCTCCAACAACATGTGTCTATTTTCAGAAATGGTTGGATTTCTGA

>MsG0780036810.01.T03

ATGAAGGCACTTATAAACAAGAGTATGGTAAAGGAACTTCCTTCCATTAAAAACAAAGGTATAAAGATCTACATGGGAACAGAATTTGAGCCTTCCAAGGATATAACTGGTGGGCATTTTTACAGCGAAGGGAGTCTTGATACGCTTTTTATAGACGCTCTAAAGACAGTGTGCTTGCAATGCATTTCATTGGAGAGAGTTTCCACATGTGATGGATGTTTGGAGTGGGTTAAAAGGAGTGGGGTCATCACAACTCAAGTCACGGGAGAACAAATAGAAGAGATTTTGCAAACTTTGGTTTTAGATGATGAGATAATGCAGATGACAAGTACTGGATATGGGGATTTTGCATCTATTCCTGTTGGCAAAACTTGTTACATATGCAAAAGCAAGGGTGAAAAGAAATCTGCTGACTCGACTCCCTTTCCATGTTTTTCTTGTCAACGGATGAGCTTTTGTTCAACGGATGGCGTTGTCTCTCCAACAACATGTGTCTATTTTCAGAAATGGTTGGATTTCTGA

>MsG0780036810.01.T02

ATGAGAAGAGAAACAAACCTTCCTATTGCTGCGCTTAATAAACACATGAAGGCACTTATAAACAAGAGTATGGTAAAGGAACTTCCTTCCATTAAAAACAAAGGTATAAAGATCTACATGGGAACAGAATTTGAGCCTTCCAAGGATATAACTGGTGGGCATTTTTACAGCGAAGGGAGTCTTGATACGCTTTTTATAGACGCTCTAAAGACAGTGTGCTTGCAATGCATTTCATTGGAGAGAGTTTCCACATGTGATGGATGTTTGGAGTGGGTTAAAAGGAGTGGGGTCATCACAACTCAAGTCACGGGAGAACAAATAGAAGAGATTTTGCAAACTTTGGTTTTAGATGATGAGATAATGCAGATGACAAGTACTGGATATGGGGATTTTGCATCTATTCCTGTTGGCAAAACTTGTTACATATGCAAAAGCAAGGGTGAAAAGAAATCTGCTGACTCGACTCCCTTTCCATGTTTTTCTTGTCAACGGATGAGCTTTTGTTCAACGGATGGCGTTGTCTCTCCAACAACATGTGTCTATTTTCAGAAATGGTTGGATTTCTGA

Protein sequence

>MsG0780036810.01.T01

MKRLQESGSSQQKRQKLSALPDEERILYDLILSKENSGISKTDMRRETNLPIAALNKHMKALINKSMVKELPSIKNKGIKIYMGTEFEPSKDITGGHFYSEGSLDTLFIDALKTVCLQCISLERVSTCDGCLEWVKRSGVITTQVTGEQIEEILQTLVLDDEIMQMTSTGYGDFASIPVGKTCYICKSKGEKKSADSTPFPCFSCQRMSFCSTDGVVSPTTCVYFQKWLDF*

>MsG0780036810.01.T03

MKALINKSMVKELPSIKNKGIKIYMGTEFEPSKDITGGHFYSEGSLDTLFIDALKTVCLQCISLERVSTCDGCLEWVKRSGVITTQVTGEQIEEILQTLVLDDEIMQMTSTGYGDFASIPVGKTCYICKSKGEKKSADSTPFPCFSCQRMSFCSTDGVVSPTTCVYFQKWLDF*

>MsG0780036810.01.T02

MRRETNLPIAALNKHMKALINKSMVKELPSIKNKGIKIYMGTEFEPSKDITGGHFYSEGSLDTLFIDALKTVCLQCISLERVSTCDGCLEWVKRSGVITTQVTGEQIEEILQTLVLDDEIMQMTSTGYGDFASIPVGKTCYICKSKGEKKSADSTPFPCFSCQRMSFCSTDGVVSPTTCVYFQKWLDF*