AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047199.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047199.01.T01 MTR_4g122900 74.457 184 8 2 1 145 1 184 6.57e-17 73.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 45 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAAATTTACCAACAATTTC+AGG 0.148545 8:-83211253 None:intergenic
TAAACTGTTGTAGGAGGATT+TGG 0.243160 8:+83210725 MsG0880047199.01.T01:intron
TATTATCATGCAACATTGTT+AGG 0.247228 8:+83211098 MsG0880047199.01.T01:three_prime_UTR
AATTTATCTTAGTAATTTGT+AGG 0.260267 8:+83211197 MsG0880047199.01.T01:three_prime_UTR
CCTCTTCAGCGTTGCCTAAA+GGG 0.272875 8:-83210767 None:intergenic
AATTGTTGGTAAATTTATTC+TGG 0.273288 8:+83211258 MsG0880047199.01.T01:three_prime_UTR
TCCTCTTCAGCGTTGCCTAA+AGG 0.387128 8:-83210768 None:intergenic
GACTACCTTGTGCGCCCTTT+AGG 0.392973 8:+83210753 MsG0880047199.01.T01:CDS
CTGCCAAGGCTCCACCAAAA+AGG 0.395839 8:+83210871 MsG0880047199.01.T01:CDS
TTCTGACACCCTGAAATTGT+TGG 0.409195 8:+83211244 MsG0880047199.01.T01:three_prime_UTR
TAGGATTTCTAGTTTCTGTC+TGG 0.412200 8:+83211117 MsG0880047199.01.T01:three_prime_UTR
ATTTCTAGTTTCTGTCTGGT+TGG 0.442891 8:+83211121 MsG0880047199.01.T01:three_prime_UTR
GTTGGTGCATAGTCGATTGT+TGG 0.451108 8:+83211139 MsG0880047199.01.T01:three_prime_UTR
AAACTGTTGTAGGAGGATTT+GGG 0.458918 8:+83210726 MsG0880047199.01.T01:intron
TAAATTTACCAACAATTTCA+GGG 0.473222 8:-83211252 None:intergenic
GGATAAAGTTGAGGAGAAAG+AGG 0.478281 8:+83209614 MsG0880047199.01.T01:CDS
GAGATAAGATGAGTAGGTCC+TGG 0.502070 8:+83211052 MsG0880047199.01.T01:three_prime_UTR
GAATTTCAAGATGAAGGTTA+CGG 0.508118 8:+83209581 None:intergenic
CGTTGCCTAAAGGGCGCACA+AGG 0.528921 8:-83210758 None:intergenic
ATATTGATCTAATTGACCTA+TGG 0.530077 8:-83211008 None:intergenic
CCCTTTAGGCAACGCTGAAG+AGG 0.547020 8:+83210767 MsG0880047199.01.T01:CDS
AGAGAGAACAAAACAACTCC+AGG 0.557428 8:-83211070 None:intergenic
GAAAATGGTGAGGAAGATGA+AGG 0.566956 8:+83210819 MsG0880047199.01.T01:CDS
CGGCGATGACGACGGAGACG+AGG 0.569029 8:+83209695 MsG0880047199.01.T01:CDS
GAAGGTTACGGAAGATTCGA+AGG 0.578457 8:+83209593 MsG0880047199.01.T01:CDS
TGTTGGTTGAAACTTCTGCT+AGG 0.580755 8:+83211156 MsG0880047199.01.T01:three_prime_UTR
TGAGAACGACGACGATGATG+AGG 0.590523 8:+83209874 MsG0880047199.01.T01:CDS
CTGGGTCTATTATATGCTTG+AGG 0.593730 8:+83210971 MsG0880047199.01.T01:three_prime_UTR
TTTAGGCAACGCTGAAGAGG+AGG 0.618802 8:+83210770 MsG0880047199.01.T01:CDS
GGACTTAACAAGTCAACCAT+AGG 0.625038 8:+83210992 MsG0880047199.01.T01:three_prime_UTR
TGATGATGAGAAGGCTGCCA+AGG 0.626187 8:+83210857 MsG0880047199.01.T01:CDS
TGAAGATGATGATGATGAGA+AGG 0.635914 8:+83210848 MsG0880047199.01.T01:CDS
CTCAAGCATATAATAGACCC+AGG 0.638779 8:-83210970 None:intergenic
TGATGAGGAGAACGATGAAG+AGG 0.641685 8:+83209889 MsG0880047199.01.T01:CDS
AGATTCGAAGGATAAAGTTG+AGG 0.642562 8:+83209605 MsG0880047199.01.T01:CDS
TGAACCAGAAGAAAATGGTG+AGG 0.647866 8:+83210809 MsG0880047199.01.T01:CDS
TTGAGTGATGAAGATGACGA+AGG 0.667428 8:+83209645 MsG0880047199.01.T01:CDS
GTAGACGACGGCGATGACGA+CGG 0.674867 8:+83209687 MsG0880047199.01.T01:CDS
TGACGAAGGAGATGATTCCG+CGG 0.677137 8:+83209659 MsG0880047199.01.T01:CDS
GATGATTCAGATGATGACGA+TGG 0.684292 8:+83210912 MsG0880047199.01.T01:CDS
GATTCAGATGATGACGATGG+AGG 0.693060 8:+83210915 MsG0880047199.01.T01:CDS
TCCGCGGAGGAAGTAGACGA+CGG 0.695976 8:+83209675 MsG0880047199.01.T01:CDS
CGAAGGAGATGATTCCGCGG+AGG 0.706879 8:+83209662 MsG0880047199.01.T01:CDS
GCCGTCGTCTACTTCCTCCG+CGG 0.720282 8:-83209676 None:intergenic
GGAGACGAGGATGATGACGA+CGG 0.760759 8:+83209708 MsG0880047199.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTAAAACTACTTTTAA+AGG - Chr8:83210016-83210035 None:intergenic 10.0%
!! ATTAACAATTTCAACAAATT+AGG - Chr8:83209948-83209967 None:intergenic 15.0%
!! TAAAACAAGTATATATAAGT+CGG - Chr8:83209918-83209937 None:intergenic 15.0%
!! TTAACAATTTCAACAAATTA+GGG - Chr8:83209947-83209966 None:intergenic 15.0%
!!! AATTTATCTTAGTAATTTGT+AGG + Chr8:83211197-83211216 MsG0880047199.01.T01:three_prime_UTR 15.0%
!!! TAATTTTTCAATTTTGAGTT+AGG + Chr8:83210029-83210048 MsG0880047199.01.T01:intron 15.0%
!! ATAAATTTACCAACAATTTC+AGG - Chr8:83211256-83211275 None:intergenic 20.0%
!! ATAATTCAATTTCACTTATG+AGG + Chr8:83210701-83210720 MsG0880047199.01.T01:intron 20.0%
!! GTACTTATAAAAAATGATAG+AGG + Chr8:83210366-83210385 MsG0880047199.01.T01:intron 20.0%
!! TAAATTTACCAACAATTTCA+GGG - Chr8:83211255-83211274 None:intergenic 20.0%
!! TAAGTGAAATTGAATTATAG+AGG - Chr8:83210699-83210718 None:intergenic 20.0%
!!! AATTGTTGGTAAATTTATTC+TGG + Chr8:83211258-83211277 MsG0880047199.01.T01:three_prime_UTR 20.0%
! ATATTGATCTAATTGACCTA+TGG - Chr8:83211011-83211030 None:intergenic 25.0%
! TATTATCATGCAACATTGTT+AGG + Chr8:83211098-83211117 MsG0880047199.01.T01:three_prime_UTR 25.0%
! TGTTCGTTAATTTCATTCAT+AGG + Chr8:83209981-83210000 MsG0880047199.01.T01:intron 25.0%
!! TTTAGAATTATGCAGTATGT+TGG + Chr8:83210341-83210360 MsG0880047199.01.T01:intron 25.0%
!!! TCAATTGTTGTGTTTTGAAT+CGG + Chr8:83210571-83210590 MsG0880047199.01.T01:intron 25.0%
GCAGCAAAATTGAATTATAC+AGG - Chr8:83210600-83210619 None:intergenic 30.0%
TGCTGCAATTAGTAGTTAAA+AGG - Chr8:83210418-83210437 None:intergenic 30.0%
TGTATGTTATGAGTTCATAG+AGG - Chr8:83210529-83210548 None:intergenic 30.0%
TTATGAGGATAAACTGTTGT+AGG + Chr8:83210716-83210735 MsG0880047199.01.T01:intron 30.0%
! GATTTTGAACCAGAAGAAAA+TGG + Chr8:83210804-83210823 MsG0880047199.01.T01:CDS 30.0%
! TTTTCAGAGATAAGATGAGT+AGG + Chr8:83211046-83211065 MsG0880047199.01.T01:three_prime_UTR 30.0%
!!! TCAATTTTGAGTTAGGATGA+AGG + Chr8:83210036-83210055 MsG0880047199.01.T01:intron 30.0%
AAACTGTTGTAGGAGGATTT+GGG + Chr8:83210726-83210745 MsG0880047199.01.T01:intron 35.0%
ACCCTCAAAAAAATAGACCT+GGG + Chr8:83210953-83210972 MsG0880047199.01.T01:exon 35.0%
AGATTCGAAGGATAAAGTTG+AGG + Chr8:83209605-83209624 MsG0880047199.01.T01:CDS 35.0%
ATTATAGAGGTAGCAGAGAT+TGG - Chr8:83210686-83210705 None:intergenic 35.0%
TAAACTGTTGTAGGAGGATT+TGG + Chr8:83210725-83210744 MsG0880047199.01.T01:intron 35.0%
! TGAAGATGATGATGATGAGA+AGG + Chr8:83210848-83210867 MsG0880047199.01.T01:CDS 35.0%
! TTATCTGACCTCTTCCTTTT+TGG - Chr8:83210888-83210907 None:intergenic 35.0%
!! ATTTCTAGTTTCTGTCTGGT+TGG + Chr8:83211121-83211140 MsG0880047199.01.T01:three_prime_UTR 35.0%
!! TAGGATTTCTAGTTTCTGTC+TGG + Chr8:83211117-83211136 MsG0880047199.01.T01:three_prime_UTR 35.0%
!!! ACCCAGGTCTATTTTTTTGA+GGG - Chr8:83210957-83210976 None:intergenic 35.0%
AGAGAGAACAAAACAACTCC+AGG - Chr8:83211073-83211092 None:intergenic 40.0%
CTCAAGCATATAATAGACCC+AGG - Chr8:83210973-83210992 None:intergenic 40.0%
CTGGGTCTATTATATGCTTG+AGG + Chr8:83210971-83210990 MsG0880047199.01.T01:three_prime_UTR 40.0%
GAAAATGGTGAGGAAGATGA+AGG + Chr8:83210819-83210838 MsG0880047199.01.T01:CDS 40.0%
GACCCTCAAAAAAATAGACC+TGG + Chr8:83210952-83210971 MsG0880047199.01.T01:exon 40.0%
GGACTTAACAAGTCAACCAT+AGG + Chr8:83210992-83211011 MsG0880047199.01.T01:three_prime_UTR 40.0%
GGATAAAGTTGAGGAGAAAG+AGG + Chr8:83209614-83209633 MsG0880047199.01.T01:CDS 40.0%
TGAACCAGAAGAAAATGGTG+AGG + Chr8:83210809-83210828 MsG0880047199.01.T01:CDS 40.0%
TGAGGATAAACTGTTGTAGG+AGG + Chr8:83210719-83210738 MsG0880047199.01.T01:intron 40.0%
TTCTGACACCCTGAAATTGT+TGG + Chr8:83211244-83211263 MsG0880047199.01.T01:three_prime_UTR 40.0%
TTGAGTGATGAAGATGACGA+AGG + Chr8:83209645-83209664 MsG0880047199.01.T01:CDS 40.0%
! GATGATTCAGATGATGACGA+TGG + Chr8:83210912-83210931 MsG0880047199.01.T01:CDS 40.0%
! TCTTCCTCACCATTTTCTTC+TGG - Chr8:83210816-83210835 None:intergenic 40.0%
! TGTTGGTTGAAACTTCTGCT+AGG + Chr8:83211156-83211175 MsG0880047199.01.T01:three_prime_UTR 40.0%
!!! GACCCAGGTCTATTTTTTTG+AGG - Chr8:83210958-83210977 None:intergenic 40.0%
GAAGGTTACGGAAGATTCGA+AGG + Chr8:83209593-83209612 MsG0880047199.01.T01:CDS 45.0%
GAGATAAGATGAGTAGGTCC+TGG + Chr8:83211052-83211071 MsG0880047199.01.T01:three_prime_UTR 45.0%
GTTGGTGCATAGTCGATTGT+TGG + Chr8:83211139-83211158 MsG0880047199.01.T01:three_prime_UTR 45.0%
TGATGAGGACGATGATGAAG+AGG + Chr8:83209731-83209750 MsG0880047199.01.T01:intron 45.0%
TGATGAGGAGAACGATGAAG+AGG + Chr8:83209889-83209908 MsG0880047199.01.T01:CDS 45.0%
! GATTCAGATGATGACGATGG+AGG + Chr8:83210915-83210934 MsG0880047199.01.T01:CDS 45.0%
! TCTGACCTCTTCCTTTTTGG+TGG - Chr8:83210885-83210904 None:intergenic 45.0%
ACGACGATGAAAGAAGCAGC+AGG + Chr8:83209806-83209825 MsG0880047199.01.T01:intron 50.0%
AGACGACGATGATGAGGATG+AGG + Chr8:83209782-83209801 MsG0880047199.01.T01:intron 50.0%
AGGCTCCACCAAAAAGGAAG+AGG + Chr8:83210877-83210896 MsG0880047199.01.T01:CDS 50.0%
AGGTAGCAGAGATTGGCAAG+CGG - Chr8:83210679-83210698 None:intergenic 50.0%
ATGAAAGAAGCAGCAGGTGC+CGG + Chr8:83209812-83209831 MsG0880047199.01.T01:intron 50.0%
CCTCTTCAGCGTTGCCTAAA+GGG - Chr8:83210770-83210789 None:intergenic 50.0%
GACGACGATGATGAGGATGA+GGG + Chr8:83209783-83209802 MsG0880047199.01.T01:intron 50.0%
TCCTCTTCAGCGTTGCCTAA+AGG - Chr8:83210771-83210790 None:intergenic 50.0%
TGACGAAGGAGATGATTCCG+CGG + Chr8:83209659-83209678 MsG0880047199.01.T01:CDS 50.0%
TGAGAACGACGACGATGATG+AGG + Chr8:83209874-83209893 MsG0880047199.01.T01:CDS 50.0%
TTTAGGCAACGCTGAAGAGG+AGG + Chr8:83210770-83210789 MsG0880047199.01.T01:CDS 50.0%
! TGATGATGAGAAGGCTGCCA+AGG + Chr8:83210857-83210876 MsG0880047199.01.T01:CDS 50.0%
!! CTTCCTTTTTGGTGGAGCCT+TGG - Chr8:83210877-83210896 None:intergenic 50.0%
CCCTTTAGGCAACGCTGAAG+AGG + Chr8:83210767-83210786 MsG0880047199.01.T01:CDS 55.0%
CTGCCAAGGCTCCACCAAAA+AGG + Chr8:83210871-83210890 MsG0880047199.01.T01:CDS 55.0%
GACTACCTTGTGCGCCCTTT+AGG + Chr8:83210753-83210772 MsG0880047199.01.T01:CDS 55.0%
TGAAGAGGATGACGACGACG+AGG + Chr8:83209746-83209765 MsG0880047199.01.T01:intron 55.0%
! GGAGACGAGGATGATGACGA+CGG + Chr8:83209708-83209727 MsG0880047199.01.T01:CDS 55.0%
! GGATGATGACGACGGTGATG+AGG + Chr8:83209716-83209735 MsG0880047199.01.T01:intron 55.0%
AGGGTCACCACCTTGCTCAC+CGG - Chr8:83209834-83209853 None:intergenic 60.0%
CATCGTCGTCGTCGTCGTCA+GGG - Chr8:83209853-83209872 None:intergenic 60.0%
CGAAGGAGATGATTCCGCGG+AGG + Chr8:83209662-83209681 MsG0880047199.01.T01:CDS 60.0%
GTAGACGACGGCGATGACGA+CGG + Chr8:83209687-83209706 MsG0880047199.01.T01:CDS 60.0%
TCATCATCGTCGTCTCCGCC+AGG - Chr8:83209777-83209796 None:intergenic 60.0%
TCATCGTCGTCGTCGTCGTC+AGG - Chr8:83209854-83209873 None:intergenic 60.0%
TCCGCGGAGGAAGTAGACGA+CGG + Chr8:83209675-83209694 MsG0880047199.01.T01:CDS 60.0%
TGGCGGAGACGACGATGATG+AGG + Chr8:83209776-83209795 MsG0880047199.01.T01:intron 60.0%
! CGTTGCCTAAAGGGCGCACA+AGG - Chr8:83210761-83210780 None:intergenic 60.0%
GACGACGACGAGGATGCTCC+TGG + Chr8:83209756-83209775 MsG0880047199.01.T01:intron 65.0%
GACGACGAGGATGCTCCTGG+CGG + Chr8:83209759-83209778 MsG0880047199.01.T01:intron 65.0%
GCCGTCGTCTACTTCCTCCG+CGG - Chr8:83209679-83209698 None:intergenic 65.0%
CGGCGATGACGACGGAGACG+AGG + Chr8:83209695-83209714 MsG0880047199.01.T01:CDS 70.0%
!! GCAGCAGGTGCCGGTGAGCA+AGG + Chr8:83209821-83209840 MsG0880047199.01.T01:intron 70.0%
!! GCAGGTGCCGGTGAGCAAGG+TGG + Chr8:83209824-83209843 MsG0880047199.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr8 gene 83209591 83211303 83209591 ID=MsG0880047199.01;Name=MsG0880047199.01
Chr8 mRNA 83209591 83211303 83209591 ID=MsG0880047199.01.T01;Parent=MsG0880047199.01;Name=MsG0880047199.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0.5|0.33|1|1|1|3|335|145
Chr8 exon 83209591 83209729 83209591 ID=MsG0880047199.01.T01:exon:20623;Parent=MsG0880047199.01.T01
Chr8 exon 83209843 83209910 83209843 ID=MsG0880047199.01.T01:exon:20624;Parent=MsG0880047199.01.T01
Chr8 exon 83210738 83211303 83210738 ID=MsG0880047199.01.T01:exon:20625;Parent=MsG0880047199.01.T01
Chr8 CDS 83209591 83209729 83209591 ID=MsG0880047199.01.T01:cds;Parent=MsG0880047199.01.T01
Chr8 CDS 83209843 83209910 83209843 ID=MsG0880047199.01.T01:cds;Parent=MsG0880047199.01.T01
Chr8 CDS 83210738 83210968 83210738 ID=MsG0880047199.01.T01:cds;Parent=MsG0880047199.01.T01
Chr8 three_prime_UTR 83210969 83211303 83210969 ID=MsG0880047199.01.T01:three_prime_utr;Parent=MsG0880047199.01.T01
Gene Sequence

>MsG0880047199.01.T01

ATGAAGGTTACGGAAGATTCGAAGGATAAAGTTGAGGAGAAAGAGGTTATCGTTTTGAGTGATGAAGATGACGAAGGAGATGATTCCGCGGAGGAAGTAGACGACGGCGATGACGACGGAGACGAGGATGATGACGACGGTGGTGACCCTGACGACGACGACGACGATGATGAGAACGACGACGATGATGAGGAGAACGATGAAGAGGAGGATTTGGGAACAGACTACCTTGTGCGCCCTTTAGGCAACGCTGAAGAGGAGGAAGCATCTAGTGATTTTGAACCAGAAGAAAATGGTGAGGAAGATGAAGGTGCTGATGAAGATGATGATGATGAGAAGGCTGCCAAGGCTCCACCAAAAAGGAAGAGGTCAGATAAAGATGATGATTCAGATGATGACGATGGAGGAGAAGATGATGAGAGACCCTCAAAAAAATAG

Protein sequence

>MsG0880047199.01.T01

MKVTEDSKDKVEEKEVIVLSDEDDEGDDSAEEVDDGDDDGDEDDDDGGDPDDDDDDDENDDDDEENDEEEDLGTDYLVRPLGNAEEEEASSDFEPEENGEEDEGADEDDDDEKAAKAPPKRKRSDKDDDSDDDDGGEDDERPSKK*