AlfalfaGEDB | MsG0580025664.01

M. sativa cultivar ZhongmuNo.1 / MsG0580025664.01

KEGG: -
GO: GO:0003700 GO:0005634 GO:0006355
PFAM: PF01486

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025664.01.T01	XP_003613086.1	79.646	113	9	1	1	99	111	223	1.77E-50	169

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025664.01.T01	Q9FVC1	54.762	126	30	2	1	99	111	236	6.88E-30	110

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025664.01.T01	G7JXQ9	79.646	113	9	1	1	99	111	223	8.46e-51	169

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048462.01	MsG0580025664.01	0.813048	3.117764e-51	2.014515e-48
MsG0080048964.01	MsG0580025664.01	0.809895	1.508960e-50	8.967215e-48
MsG0180000065.01	MsG0580025664.01	0.803384	3.577114e-49	1.795038e-46
MsG0180000235.01	MsG0580025664.01	0.811196	7.899626e-51	4.858800e-48
MsG0180000470.01	MsG0580025664.01	0.823344	1.457187e-53	1.251265e-50
MsG0180000555.01	MsG0580025664.01	0.818309	2.096439e-52	1.563690e-49
MsG0180000585.01	MsG0580025664.01	0.809722	1.644095e-50	9.726506e-48
MsG0180000822.01	MsG0580025664.01	0.832608	8.580041e-56	9.638719e-53
MsG0180000855.01	MsG0580025664.01	0.806708	7.213503e-50	3.942405e-47
MsG0180002894.01	MsG0580025664.01	0.830270	3.227594e-55	3.384064e-52
MsG0180002955.01	MsG0580025664.01	0.803161	3.977484e-49	1.984646e-46
MsG0180003031.01	MsG0580025664.01	0.810755	9.845064e-51	5.985126e-48
MsG0180003669.01	MsG0580025664.01	0.810851	9.384762e-51	5.719982e-48
MsG0180004388.01	MsG0580025664.01	0.836588	8.569694e-57	1.084771e-53
MsG0180004449.01	MsG0580025664.01	0.829624	4.637680e-55	4.772412e-52
MsG0180004689.01	MsG0580025664.01	0.842612	2.325647e-58	3.545406e-55
MsG0180005050.01	MsG0580025664.01	0.816665	4.919284e-52	3.507496e-49
MsG0180005233.01	MsG0580025664.01	0.808764	2.638738e-50	1.521606e-47
MsG0180005250.01	MsG0580025664.01	0.809871	1.527352e-50	9.070988e-48
MsG0180005439.01	MsG0580025664.01	0.812414	4.290535e-51	2.726419e-48
MsG0180005507.01	MsG0580025664.01	0.801054	1.079140e-48	5.104203e-46
MsG0180005842.01	MsG0580025664.01	0.815431	9.279566e-52	6.396559e-49
MsG0180005944.01	MsG0580025664.01	0.851899	6.570616e-61	1.349504e-57
MsG0180005993.01	MsG0580025664.01	0.814417	1.556874e-51	1.044113e-48
MsG0180006016.01	MsG0580025664.01	0.830757	2.453489e-55	2.609368e-52
MsG0180006155.01	MsG0580025664.01	0.802815	4.691152e-49	2.320263e-46
MsG0280006375.01	MsG0580025664.01	0.807665	4.522859e-50	2.534036e-47
MsG0280010560.01	MsG0580025664.01	0.814645	1.386162e-51	9.353377e-49
MsG0280010614.01	MsG0580025664.01	0.846095	2.694001e-59	4.585702e-56
MsG0280010698.01	MsG0580025664.01	0.815336	9.742633e-52	6.698250e-49
MsG0280010778.01	MsG0580025664.01	0.815810	7.639969e-52	5.321415e-49
MsG0280011043.01	MsG0580025664.01	0.812526	4.055231e-51	2.584495e-48
MsG0280011052.01	MsG0580025664.01	0.818127	2.305358e-52	1.710886e-49
MsG0280011141.01	MsG0580025664.01	0.810912	9.102253e-51	5.556928e-48
MsG0280011235.01	MsG0580025664.01	0.801044	1.083847e-48	5.125285e-46
MsG0280011334.01	MsG0580025664.01	0.817367	3.420716e-52	2.486309e-49
MsG0280011446.01	MsG0580025664.01	0.820648	6.138597e-53	4.885062e-50
MsG0380011663.01	MsG0580025664.01	0.823191	1.581567e-53	1.352343e-50
MsG0380012460.01	MsG0580025664.01	0.806755	7.049682e-50	3.857560e-47
MsG0380013176.01	MsG0580025664.01	0.802061	6.706665e-49	3.253894e-46
MsG0380014537.01	MsG0580025664.01	0.833136	6.340693e-56	7.237212e-53
MsG0380014573.01	MsG0580025664.01	0.831207	1.902335e-55	2.050163e-52
MsG0380014701.01	MsG0580025664.01	0.803409	3.534385e-49	1.774772e-46
MsG0480018640.01	MsG0580025664.01	0.832181	1.094181e-55	1.213689e-52
MsG0480018787.01	MsG0580025664.01	0.822413	2.401149e-53	2.008420e-50
MsG0480018970.01	MsG0580025664.01	0.835830	1.335039e-56	1.651854e-53
MsG0480019970.01	MsG0580025664.01	0.804346	2.258191e-49	1.161569e-46
MsG0480020447.01	MsG0580025664.01	0.819296	1.251054e-52	9.588686e-50
MsG0480020720.01	MsG0580025664.01	0.822508	2.282321e-53	1.914147e-50
MsG0480021215.01	MsG0580025664.01	0.817225	3.681678e-52	2.665549e-49
MsG0480021240.01	MsG0580025664.01	0.800378	1.482187e-48	6.891193e-46
MsG0480021465.01	MsG0580025664.01	0.809237	2.089402e-50	1.220257e-47
MsG0480021953.01	MsG0580025664.01	0.812393	4.336991e-51	2.754340e-48
MsG0480021994.01	MsG0580025664.01	0.801851	7.409991e-49	3.575940e-46
MsG0480022292.01	MsG0580025664.01	0.800629	1.317343e-48	6.163832e-46
MsG0480022318.01	MsG0580025664.01	0.802726	4.893393e-49	2.414776e-46
MsG0480022467.01	MsG0580025664.01	0.824780	6.706694e-54	5.999756e-51
MsG0480022480.01	MsG0580025664.01	0.808716	2.701630e-50	1.555870e-47
MsG0480022985.01	MsG0580025664.01	0.808165	3.541482e-50	2.010533e-47
MsG0480023014.01	MsG0580025664.01	0.806260	8.968529e-50	4.846054e-47
MsG0480023088.01	MsG0580025664.01	0.817664	2.933213e-52	2.149401e-49
MsG0480023224.01	MsG0580025664.01	0.812460	4.193773e-51	2.668161e-48
MsG0480023245.01	MsG0580025664.01	0.815344	9.701884e-52	6.671772e-49
MsG0480023258.01	MsG0580025664.01	0.800796	1.218009e-48	5.723067e-46
MsG0480023412.01	MsG0580025664.01	0.827196	1.787473e-54	1.713636e-51
MsG0480023415.01	MsG0580025664.01	0.824224	9.065296e-54	7.980583e-51
MsG0480023517.01	MsG0580025664.01	0.813120	3.006209e-51	1.946109e-48
MsG0480023554.01	MsG0580025664.01	0.813337	2.693227e-51	1.753999e-48
MsG0480023600.01	MsG0580025664.01	0.801487	8.800098e-49	4.207779e-46
MsG0480023670.01	MsG0580025664.01	0.802064	6.698091e-49	3.249927e-46
MsG0480023873.01	MsG0580025664.01	0.816667	4.912892e-52	3.503149e-49
MsG0580024114.01	MsG0580025664.01	0.813608	2.348977e-51	1.541078e-48
MsG0580024374.01	MsG0580025664.01	0.810785	9.697943e-51	5.900341e-48
MsG0580024418.01	MsG0580025664.01	0.812803	3.527321e-51	2.264197e-48
MsG0580024536.01	MsG0580025664.01	0.804050	2.601575e-49	1.328120e-46
MsG0580024733.01	MsG0580025664.01	0.843870	1.073615e-58	1.702860e-55
MsG0580024781.01	MsG0580025664.01	0.806766	7.013063e-50	3.838610e-47
MsG0580025008.01	MsG0580025664.01	0.805973	1.030770e-49	5.528878e-47
MsG0580025009.01	MsG0580025664.01	0.825167	5.432721e-54	4.914134e-51
MsG0580025022.01	MsG0580025664.01	0.800431	1.445973e-48	6.731805e-46
MsG0580025026.01	MsG0580025664.01	0.804891	1.737683e-49	9.065914e-47
MsG0580025328.01	MsG0580025664.01	0.803641	3.163388e-49	1.597996e-46
MsG0580025489.01	MsG0580025664.01	0.801461	8.905314e-49	4.255331e-46
MsG0580025651.01	MsG0580025664.01	0.848908	4.541792e-60	8.463796e-57
MsG0580025664.01	MsG0580025899.01	0.826523	2.589610e-54	2.434361e-51
MsG0580025664.01	MsG0580026225.01	0.814032	1.893706e-51	1.256756e-48
MsG0580025664.01	MsG0580028969.01	0.808113	3.633044e-50	2.059572e-47
MsG0580025664.01	MsG0580029008.01	0.810169	1.317113e-50	7.883452e-48
MsG0580025664.01	MsG0580029189.01	0.820486	6.688289e-53	5.299020e-50
MsG0580025664.01	MsG0580029594.01	0.807741	4.359524e-50	2.447439e-47
MsG0580025664.01	MsG0580029762.01	0.853943	1.710467e-61	3.758460e-58
MsG0580025664.01	MsG0580029794.01	0.805795	1.123283e-49	5.997678e-47
MsG0580025664.01	MsG0580030235.01	0.832094	1.149684e-55	1.272023e-52
MsG0580025664.01	MsG0680030685.01	0.840762	7.152039e-58	1.029053e-54
MsG0580025664.01	MsG0680031506.01	0.802648	5.078354e-49	2.500896e-46
MsG0580025664.01	MsG0680031651.01	0.844265	8.416154e-59	1.351340e-55
MsG0580025664.01	MsG0680034037.01	0.811859	5.669837e-51	3.548885e-48
MsG0580025664.01	MsG0680035340.01	0.805553	1.262571e-49	6.699265e-47
MsG0580025664.01	MsG0680035573.01	0.826368	2.819336e-54	2.638835e-51
MsG0580025664.01	MsG0680035844.01	0.820829	5.576754e-53	4.460912e-50
MsG0580025664.01	MsG0780036773.01	0.801736	7.822515e-49	3.763822e-46
MsG0580025664.01	MsG0780037287.01	0.831318	1.786611e-55	1.931851e-52
MsG0580025664.01	MsG0780038401.01	0.818485	1.912213e-52	1.433271e-49
MsG0580025664.01	MsG0780040176.01	0.823428	1.392916e-53	1.198915e-50
MsG0580025664.01	MsG0780040262.01	0.816868	4.430183e-52	3.176403e-49
MsG0580025664.01	MsG0780040704.01	0.800085	1.700642e-48	7.848035e-46
MsG0580025664.01	MsG0780040712.01	0.801769	7.700097e-49	3.708086e-46
MsG0580025664.01	MsG0780040726.01	0.820305	7.358108e-53	5.800665e-50
MsG0580025664.01	MsG0780041161.01	0.831184	1.927583e-55	2.075965e-52
MsG0580025664.01	MsG0780041305.01	0.800332	1.514479e-48	7.033120e-46
MsG0580025664.01	MsG0780041456.01	0.806364	8.527589e-50	4.619675e-47
MsG0580025664.01	MsG0780041469.01	0.808919	2.444164e-50	1.415215e-47
MsG0580025664.01	MsG0880042698.01	0.809907	1.499953e-50	8.916779e-48
MsG0580025664.01	MsG0880042939.01	0.814950	1.186596e-51	8.072336e-49
MsG0580025664.01	MsG0880043059.01	0.809130	2.203428e-50	1.283140e-47
MsG0580025664.01	MsG0880043116.01	0.808803	2.588505e-50	1.494167e-47
MsG0580025664.01	MsG0880043522.01	0.802039	6.778442e-49	3.286792e-46
MsG0580025664.01	MsG0880043532.01	0.813233	2.839419e-51	1.843807e-48
MsG0580025664.01	MsG0880044574.01	0.802988	4.319655e-49	2.145733e-46
MsG0580025664.01	MsG0880045106.01	0.820999	5.096927e-53	4.096818e-50
MsG0580025664.01	MsG0880045613.01	0.804311	2.295597e-49	1.179758e-46
MsG0580025664.01	MsG0880045736.01	0.812323	4.492144e-51	2.847407e-48
MsG0580025664.01	MsG0880045937.01	0.808054	3.739334e-50	2.116496e-47
MsG0580025664.01	MsG0880046020.01	0.810363	1.196315e-50	7.196924e-48
MsG0580025664.01	MsG0880046027.01	0.801264	9.772688e-49	4.646727e-46
MsG0580025664.01	MsG0880046244.01	0.803128	4.041965e-49	2.014989e-46
MsG0580025664.01	MsG0880047019.01	0.807001	6.255275e-50	3.444732e-47
MsG0580025664.01	MsG0880047143.01	0.817248	3.639505e-52	2.636625e-49
MsG0580025664.01	MsG0880047192.01	0.802746	4.847164e-49	2.393120e-46
MsG0580025664.01	MsG0880047199.01	0.809741	1.628790e-50	9.640872e-48
MsG0380015555.01	MsG0580025664.01	0.807310	5.379852e-50	2.986650e-47
MsG0380015779.01	MsG0580025664.01	0.800100	1.688652e-48	7.795534e-46
MsG0380015830.01	MsG0580025664.01	0.814868	1.237546e-51	8.400324e-49
MsG0380016227.01	MsG0580025664.01	0.831525	1.588716e-55	1.728577e-52
MsG0380016859.01	MsG0580025664.01	0.847111	1.421797e-59	2.501078e-56
MsG0380016971.01	MsG0580025664.01	0.829534	4.878282e-55	5.006695e-52
MsG0380017140.01	MsG0580025664.01	0.831297	1.808436e-55	1.954151e-52
MsG0380017375.01	MsG0580025664.01	0.807879	4.074205e-50	2.295374e-47
MsG0380017604.01	MsG0580025664.01	0.826124	3.222183e-54	2.994874e-51
MsG0380017631.01	MsG0580025664.01	0.829807	4.187179e-55	4.332617e-52
MsG0380017680.01	MsG0580025664.01	0.818086	2.354633e-52	1.745404e-49
MsG0380018009.01	MsG0580025664.01	0.803068	4.158783e-49	2.069986e-46
MsG0480018163.01	MsG0580025664.01	0.819527	1.108361e-52	8.550733e-50
MsG0280007088.01	MsG0580025664.01	0.805069	1.594804e-49	8.357948e-47
MsG0280007374.01	MsG0580025664.01	0.804834	1.786294e-49	9.305267e-47
MsG0280007529.01	MsG0580025664.01	0.829736	4.356959e-55	4.498687e-52
MsG0280007585.01	MsG0580025664.01	0.822573	2.203699e-53	1.851810e-50
MsG0280007710.01	MsG0580025664.01	0.812298	4.547950e-51	2.880870e-48
MsG0280008112.01	MsG0580025664.01	0.811525	6.702633e-51	4.158628e-48
MsG0280008295.01	MsG0580025664.01	0.803259	3.796781e-49	1.899190e-46
MsG0280008502.01	MsG0580025664.01	0.817974	2.496089e-52	1.844616e-49
MsG0280008556.01	MsG0580025664.01	0.817684	2.901728e-52	2.127519e-49
MsG0280009024.01	MsG0580025664.01	0.817782	2.758252e-52	2.027774e-49
MsG0280009235.01	MsG0580025664.01	0.816638	4.986892e-52	3.553007e-49
MsG0280009441.01	MsG0580025664.01	0.808063	3.723170e-50	2.107884e-47
MsG0280010233.01	MsG0580025664.01	0.810835	9.460523e-51	5.763603e-48
MsG0280010416.01	MsG0580025664.01	0.810706	1.008569e-50	6.123420e-48
MsG0280010447.01	MsG0580025664.01	0.803378	3.586798e-49	1.799624e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025664.01.T01	MTR_5g032520	79.646	113	9	1	1	99	111	223	2.15e-54	169
MsG0580025664.01.T01	MTR_5g032150	65.789	114	24	2	1	99	50	163	7.73e-42	135
MsG0580025664.01.T01	MTR_5g032150	65.789	114	24	2	1	99	111	224	2.00e-41	136
MsG0580025664.01.T01	MTR_5g066180	43.363	113	50	3	1	99	109	221	4.29e-15	68.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025664.01.T01	AT2G22540	54.762	126	30	2	1	99	106	231	6.96e-31	110
MsG0580025664.01.T01	AT2G22540	54.762	126	30	2	1	99	111	236	7.01e-31	110
MsG0580025664.01.T01	AT2G22540	54.762	126	30	2	1	99	111	236	7.01e-31	110
MsG0580025664.01.T01	AT4G24540	44.643	112	51	5	1	103	111	220	5.84e-16	70.9

Find 64 sgRNAs with CRISPR-Local

Find 323 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGTGTAAGTTGACTATTTA+TGG	0.095568	5:-22231250	None:intergenic
CTCGGACACCTCACTCAAAT+TGG	0.204382	5:+22236548	MsG0580025664.01.T01:CDS
CACTCTTTCTCTCTCTTCAT+TGG	0.247557	5:+22230913	MsG0580025664.01.T01:five_prime_UTR
TTGGAGAAGTCTCTTGAAAC+TGG	0.275036	5:+22234054	MsG0580025664.01.T01:CDS
ATCCAAAAGAGGATAAATTA+AGG	0.281250	5:+22231429	MsG0580025664.01.T01:intron
TCTCATCATTTCTCTTCTAC+TGG	0.281390	5:+22231593	MsG0580025664.01.T01:five_prime_UTR
AAGAAAAGAAACTTAGGTTT+TGG	0.281619	5:-22230984	None:intergenic
TGTTGTGGGTGGTGAGGGTT+TGG	0.314850	5:-22231052	None:intergenic
TTTGTGGAGGCGTTGGTATT+TGG	0.332464	5:-22231014	None:intergenic
CTCAGAGAAAGCATCAATTA+AGG	0.355556	5:+22233980	MsG0580025664.01.T01:five_prime_UTR
TCTGGAAGAATTACAACAAT+TGG	0.380411	5:+22234035	MsG0580025664.01.T01:CDS
TCGGACACCTCACTCAAATT+GGG	0.384967	5:+22236549	MsG0580025664.01.T01:CDS
AAGAAGAGGATTAATCAAAA+AGG	0.392982	5:+22231533	MsG0580025664.01.T01:five_prime_UTR
GAAATTAACTAGTGAAGAAA+TGG	0.398177	5:-22231114	None:intergenic
TCTTTGAATACTCCAACTTA+AGG	0.399297	5:+22231621	MsG0580025664.01.T01:five_prime_UTR
AAAACCGTCGCTAGATTTGC+AGG	0.401947	5:+22233169	MsG0580025664.01.T01:five_prime_UTR
GTGGGTGGTGAGGGTTTGGA+AGG	0.425658	5:-22231048	None:intergenic
TAGGTGACGGACATGTTTAA+TGG	0.427034	5:+22236414	MsG0580025664.01.T01:intron
TCTTTCTCTCTCTTCATTGG+TGG	0.439600	5:+22230916	MsG0580025664.01.T01:five_prime_UTR
AATTGTGTTGTGGGTGGTGA+GGG	0.444309	5:-22231057	None:intergenic
GAGAAAGAAAGACCTTAAGT+TGG	0.454100	5:-22231633	None:intergenic
ATGATAAAGAAAAGAAACTT+AGG	0.463704	5:-22230990	None:intergenic
ATTCTTCCAGACTCAAACCT+TGG	0.464341	5:-22234023	None:intergenic
AGATCTCCAAGGTTTGAGTC+TGG	0.468779	5:+22234017	MsG0580025664.01.T01:CDS
CAATGACACATTGAATGAAA+TGG	0.468787	5:-22236603	None:intergenic
TAATATAGAAATTGTGTTGT+GGG	0.468801	5:-22231066	None:intergenic
TCAGGGAGAGAAGATTATGA+TGG	0.473941	5:+22234177	MsG0580025664.01.T01:intron
CTCATCATTTCTCTTCTACT+GGG	0.477556	5:+22231594	MsG0580025664.01.T01:five_prime_UTR
AATTAAGGTAAAGAAAGATA+TGG	0.481532	5:+22231444	MsG0580025664.01.T01:five_prime_UTR
AAGTCTCTTGAAACTGGGTT+GGG	0.490075	5:+22234060	MsG0580025664.01.T01:CDS
CACTGAAGAAGGTCAATCTT+CGG	0.493342	5:+22236473	MsG0580025664.01.T01:CDS
ATGTTTAATGGTAAAAGTCT+TGG	0.495486	5:+22236426	MsG0580025664.01.T01:CDS
GAAGTCTCTTGAAACTGGGT+TGG	0.501534	5:+22234059	MsG0580025664.01.T01:CDS
TGGGTGGTGAGGGTTTGGAA+GGG	0.512771	5:-22231047	None:intergenic
TTTAATGGTAAAAGTCTTGG+AGG	0.517414	5:+22236429	MsG0580025664.01.T01:CDS
ATTGTACTTTGTGGAGGCGT+TGG	0.524049	5:-22231021	None:intergenic
GAGAACGTGGTCACTGAAGA+AGG	0.533782	5:+22236462	MsG0580025664.01.T01:CDS
ATTAACTAGTGAAGAAATGG+TGG	0.533811	5:-22231111	None:intergenic
GAAGGGTGAATTGTACTTTG+TGG	0.544904	5:-22231030	None:intergenic
TTTGTTTGTTTGGCTTTGTG+AGG	0.550317	5:-22230957	None:intergenic
TCCACAGGACTATGAGAGCT+CGG	0.560672	5:+22236530	MsG0580025664.01.T01:CDS
AGCATCAATTAAGGCAGATG+AGG	0.561688	5:+22233989	MsG0580025664.01.T01:exon
GATCAATGAGCTACAAACAA+AGG	0.563866	5:+22234201	MsG0580025664.01.T01:CDS
AAATTGTGTTGTGGGTGGTG+AGG	0.566310	5:-22231058	None:intergenic
AGACCTTGGTTTCAATTACA+CGG	0.567331	5:-22234083	None:intergenic
TGGAGAAGTCTCTTGAAACT+GGG	0.575456	5:+22234055	MsG0580025664.01.T01:CDS
CAACTCTACTAGTCCTCCAC+AGG	0.580687	5:+22236515	MsG0580025664.01.T01:CDS
GCATCAATTAAGGCAGATGA+GGG	0.582807	5:+22233990	MsG0580025664.01.T01:exon
TTAATATAGAAATTGTGTTG+TGG	0.595267	5:-22231067	None:intergenic
CAACTGTTCTAGACTGAGCA+AGG	0.612785	5:+22233952	MsG0580025664.01.T01:five_prime_UTR
GGGTGAATTGTACTTTGTGG+AGG	0.615816	5:-22231027	None:intergenic
TTTATAGGTCTGAAGTGATG+AGG	0.619657	5:-22231136	None:intergenic
TCCGAGCTCTCATAGTCCTG+TGG	0.619993	5:-22236531	None:intergenic
CGGACACCTCACTCAAATTG+GGG	0.635816	5:+22236550	MsG0580025664.01.T01:CDS
GAGCTCTCATAGTCCTGTGG+AGG	0.647005	5:-22236528	None:intergenic
GGGCCGTGTAATTGAAACCA+AGG	0.647098	5:+22234080	MsG0580025664.01.T01:CDS
AGATTGAAAACGCAACGGCG+AGG	0.667280	5:+22231491	MsG0580025664.01.T01:five_prime_UTR
AACTAACCCCAATTTGAGTG+AGG	0.674292	5:-22236556	None:intergenic
AAAGAAGATTGAAAACGCAA+CGG	0.680027	5:+22231486	MsG0580025664.01.T01:five_prime_UTR
TATAGAAATTGTGTTGTGGG+TGG	0.680894	5:-22231063	None:intergenic
CCAACCTGCAAATCTAGCGA+CGG	0.687238	5:-22233173	None:intergenic
ATGAGGGGAGAAGATCTCCA+AGG	0.693273	5:+22234006	MsG0580025664.01.T01:CDS
CATCAATTAAGGCAGATGAG+GGG	0.712596	5:+22233991	MsG0580025664.01.T01:exon
AGGAGTTGAATATGAGAACG+TGG	0.723449	5:+22236449	MsG0580025664.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAAAAATAATAATATAA+AGG	+	Chr5:22232076-22232095	MsG0580025664.01.T01:intron	0.0%
!!	AAAAAAATGTAGTATTAATT+AGG	+	Chr5:22234841-22234860	MsG0580025664.01.T01:intron	10.0%
!!	AAAAAATGTAGTATTAATTA+GGG	+	Chr5:22234842-22234861	MsG0580025664.01.T01:intron	10.0%
!!	AAAATTAGAGTTATAATTAA+TGG	-	Chr5:22236103-22236122	None:intergenic	10.0%
!!	AATTTAATACATATACAAAA+AGG	-	Chr5:22234806-22234825	None:intergenic	10.0%
!!	ATTAATAAATAGTTATTGTT+GGG	+	Chr5:22232515-22232534	MsG0580025664.01.T01:intron	10.0%
!!	GAAAACAATAAAAAATTTAA+GGG	+	Chr5:22232802-22232821	MsG0580025664.01.T01:intron	10.0%
!!!	TATTTTTATTTTTGCATATA+TGG	+	Chr5:22235478-22235497	MsG0580025664.01.T01:intron	10.0%
!!	AAAAATGTAGTATTAATTAG+GGG	+	Chr5:22234843-22234862	MsG0580025664.01.T01:intron	15.0%
!!	AATTATTTAACAACTAATGT+CGG	-	Chr5:22233802-22233821	None:intergenic	15.0%
!!	ATACAAACACTTTATATTAA+AGG	-	Chr5:22232872-22232891	None:intergenic	15.0%
!!	ATATAATAGTCTATTATTTG+TGG	+	Chr5:22231977-22231996	MsG0580025664.01.T01:intron	15.0%
!!	CACACAATATTTATATTATA+AGG	+	Chr5:22235008-22235027	MsG0580025664.01.T01:intron	15.0%
!!	CATTAATAAATAGTTATTGT+TGG	+	Chr5:22232514-22232533	MsG0580025664.01.T01:intron	15.0%
!!	GGAAAACAATAAAAAATTTA+AGG	+	Chr5:22232801-22232820	MsG0580025664.01.T01:intron	15.0%
!!	GTTAAATAATTACAAATCTT+TGG	+	Chr5:22233811-22233830	MsG0580025664.01.T01:intron	15.0%
!!	TAAAAAATTTAAGGGAATAT+AGG	+	Chr5:22232810-22232829	MsG0580025664.01.T01:intron	15.0%
!!	TATATGTAGCAAAAAAATAA+AGG	+	Chr5:22231376-22231395	MsG0580025664.01.T01:intron	15.0%
!!	TTAGTGAAAATAATAGTAAT+GGG	-	Chr5:22231687-22231706	None:intergenic	15.0%
!!	TTTAGTGAAAATAATAGTAA+TGG	-	Chr5:22231688-22231707	None:intergenic	15.0%
!!!	TATAAATATTGTGTGTTTTT+CGG	-	Chr5:22235003-22235022	None:intergenic	15.0%
!!!	TATATACTTAATTAGGATTT+TGG	-	Chr5:22234679-22234698	None:intergenic	15.0%
!!!	TGTGTACAATAATTTTTATA+TGG	+	Chr5:22233849-22233868	MsG0580025664.01.T01:intron	15.0%
!!!	TGTTTAGTATTTTTTACTAA+AGG	-	Chr5:22233761-22233780	None:intergenic	15.0%
!!!	TTTTAATAATAGTACCTAAT+AGG	-	Chr5:22232587-22232606	None:intergenic	15.0%
!!	AAAGAAAATCTTGAATAGAT+TGG	+	Chr5:22233307-22233326	MsG0580025664.01.T01:intron	20.0%
!!	AACTAACTATTATTTGATAG+AGG	-	Chr5:22233542-22233561	None:intergenic	20.0%
!!	AAGTAGAGAAAAAAATAATG+TGG	+	Chr5:22234404-22234423	MsG0580025664.01.T01:intron	20.0%
!!	AATGTGGGAAAAAAAAAATT+GGG	+	Chr5:22232388-22232407	MsG0580025664.01.T01:intron	20.0%
!!	AATTAAGGTAAAGAAAGATA+TGG	+	Chr5:22231444-22231463	MsG0580025664.01.T01:five_prime_UTR	20.0%
!!	AATTGTCACATATCATTATT+AGG	-	Chr5:22236282-22236301	None:intergenic	20.0%
!!	ATAAAATATTGAATGAGTGA+AGG	+	Chr5:22231937-22231956	MsG0580025664.01.T01:intron	20.0%
!!	ATAATTACAAATCTTTGGAT+AGG	+	Chr5:22233816-22233835	MsG0580025664.01.T01:intron	20.0%
!!	ATGATAAAGAAAAGAAACTT+AGG	-	Chr5:22230993-22231012	None:intergenic	20.0%
!!	ATTAAATTTAGCATCAAACA+TGG	-	Chr5:22233912-22233931	None:intergenic	20.0%
!!	TAATATAGAAATTGTGTTGT+GGG	-	Chr5:22231069-22231088	None:intergenic	20.0%
!!	TCTTGATGTAAAATAGATAT+GGG	-	Chr5:22231843-22231862	None:intergenic	20.0%
!!	TGAAACTATATATAAGGAAA+AGG	+	Chr5:22234538-22234557	MsG0580025664.01.T01:intron	20.0%
!!	TGTGATTGAAACTATATATA+AGG	+	Chr5:22234532-22234551	MsG0580025664.01.T01:intron	20.0%
!!	TTAATATAGAAATTGTGTTG+TGG	-	Chr5:22231070-22231089	None:intergenic	20.0%
!!	TTACATCAAGAGATTATAAA+TGG	+	Chr5:22231852-22231871	MsG0580025664.01.T01:intron	20.0%
!!!	AAATAATAAGACCTTTTCAT+AGG	+	Chr5:22233662-22233681	MsG0580025664.01.T01:intron	20.0%
!!!	AATAATAAGACCTTTTCATA+GGG	+	Chr5:22233663-22233682	MsG0580025664.01.T01:intron	20.0%
!!!	AGCTAATTTTAGTTTTGTTT+AGG	-	Chr5:22231164-22231183	None:intergenic	20.0%
!!!	AGTTTTGTTTAGGAATTTAT+AGG	-	Chr5:22231154-22231173	None:intergenic	20.0%
!!!	ATGGTGGATTTATTTATTTT+TGG	-	Chr5:22231098-22231117	None:intergenic	20.0%
!!!	ATGTGATGTTATATATGTTT+TGG	-	Chr5:22235911-22235930	None:intergenic	20.0%
!!!	ATTATTGTTTTATCCAAAAG+AGG	+	Chr5:22231418-22231437	MsG0580025664.01.T01:intron	20.0%
!!!	ATTTTATTGAAAAGTAGTGT+GGG	-	Chr5:22232464-22232483	None:intergenic	20.0%
!!!	ATTTTCACAGCTTTTAAAAT+AGG	+	Chr5:22235580-22235599	MsG0580025664.01.T01:intron	20.0%
!!!	TATTTTATTGAAAAGTAGTG+TGG	-	Chr5:22232465-22232484	None:intergenic	20.0%
!!!	TTTTTCTATGTCCTAAATAA+CGG	-	Chr5:22234339-22234358	None:intergenic	20.0%
!	AAGAAAAAAAAATGATGCCA+TGG	+	Chr5:22231893-22231912	MsG0580025664.01.T01:intron	25.0%
!	AAGAAGAGGATTAATCAAAA+AGG	+	Chr5:22231533-22231552	MsG0580025664.01.T01:five_prime_UTR	25.0%
!	AATGAGTGAAGGAAAATTAA+AGG	+	Chr5:22231948-22231967	MsG0580025664.01.T01:intron	25.0%
!	ACAAAAATAAAGTCAAATGG+TGG	+	Chr5:22234926-22234945	MsG0580025664.01.T01:intron	25.0%
!	AGGACATAGAAAAATGTTAT+TGG	+	Chr5:22234345-22234364	MsG0580025664.01.T01:intron	25.0%
!	ATCCAAAAGAGGATAAATTA+AGG	+	Chr5:22231429-22231448	MsG0580025664.01.T01:intron	25.0%
!	ATGTGGGAAAAAAAAAATTG+GGG	+	Chr5:22232389-22232408	MsG0580025664.01.T01:intron	25.0%
!	ATGTTTAATGGTAAAAGTCT+TGG	+	Chr5:22236426-22236445	MsG0580025664.01.T01:CDS	25.0%
!	CAATGTGGGAAAAAAAAAAT+TGG	+	Chr5:22232387-22232406	MsG0580025664.01.T01:intron	25.0%
!	CATATACAAAAAGGACAATA+TGG	-	Chr5:22234797-22234816	None:intergenic	25.0%
!	CCAAGTATATAGTAGATATA+TGG	-	Chr5:22234377-22234396	None:intergenic	25.0%
!	CCATATATCTACTATATACT+TGG	+	Chr5:22234374-22234393	MsG0580025664.01.T01:intron	25.0%
!	CTCTTGATGTAAAATAGATA+TGG	-	Chr5:22231844-22231863	None:intergenic	25.0%
!	GAAATTAACTAGTGAAGAAA+TGG	-	Chr5:22231117-22231136	None:intergenic	25.0%
!	GCAAGCAAACATATATAATA+TGG	-	Chr5:22233619-22233638	None:intergenic	25.0%
!	GCGACAAATTAAATTTCATA+GGG	+	Chr5:22235335-22235354	MsG0580025664.01.T01:intron	25.0%
!	GTTTAAGAACTCAAATGTAA+GGG	+	Chr5:22232652-22232671	MsG0580025664.01.T01:intron	25.0%
!	TAAAATCACTTGTATGTGTT+AGG	+	Chr5:22236395-22236414	MsG0580025664.01.T01:intron	25.0%
!	TAACAAAGGACATTGTATAT+AGG	+	Chr5:22235663-22235682	MsG0580025664.01.T01:intron	25.0%
!	TAATACTAATCAACACAGTT+TGG	-	Chr5:22231720-22231739	None:intergenic	25.0%
!	TCATCTATCAAAAGTCTAAA+TGG	-	Chr5:22232542-22232561	None:intergenic	25.0%
!	TGAAAATATGTGTAAGTTGT+AGG	-	Chr5:22232186-22232205	None:intergenic	25.0%
!	TGAAACATTTCTCATAGTTT+CGG	+	Chr5:22233872-22233891	MsG0580025664.01.T01:intron	25.0%
!	TGGAATCACATAAAGTATAA+AGG	-	Chr5:22235292-22235311	None:intergenic	25.0%
!	TGTTGGAATATGTGTTTATT+CGG	-	Chr5:22235971-22235990	None:intergenic	25.0%
!!	AAGAAAAGAAACTTAGGTTT+TGG	-	Chr5:22230987-22231006	None:intergenic	25.0%
!!	ATAATAAGACCTTTTCATAG+GGG	+	Chr5:22233664-22233683	MsG0580025664.01.T01:intron	25.0%
!!	ATGTGTAAGTTGACTATTTA+TGG	-	Chr5:22231253-22231272	None:intergenic	25.0%
!!	CTTACATTTGAGTTCTTAAA+CGG	-	Chr5:22232653-22232672	None:intergenic	25.0%
!!	TACCTTAATTTATCCTCTTT+TGG	-	Chr5:22231434-22231453	None:intergenic	25.0%
!!	TGTCCTTTGTTATGTTATTT+CGG	-	Chr5:22235655-22235674	None:intergenic	25.0%
!!	TTTTATTGAAAAGTAGTGTG+GGG	-	Chr5:22232463-22232482	None:intergenic	25.0%
!!!	AACATATGATTTGTGGTTTT+AGG	-	Chr5:22233454-22233473	None:intergenic	25.0%
!!!	ATATGTTTTGGTTAGTATGA+TGG	-	Chr5:22235899-22235918	None:intergenic	25.0%
!!!	CAATTTTTTTTTTCCCACAT+TGG	-	Chr5:22232389-22232408	None:intergenic	25.0%
!!!	TGTTTGGAAGTTTTTTTTTG+AGG	-	Chr5:22235836-22235855	None:intergenic	25.0%
	AAAAAAGTGATGTGTACATG+TGG	+	Chr5:22232613-22232632	MsG0580025664.01.T01:intron	30.0%
	AAAAAGTGATGTGTACATGT+GGG	+	Chr5:22232614-22232633	MsG0580025664.01.T01:intron	30.0%
	AAAAGAGGTTACATTAAGGA+CGG	-	Chr5:22234708-22234727	None:intergenic	30.0%
	AAAGAAGATTGAAAACGCAA+CGG	+	Chr5:22231486-22231505	MsG0580025664.01.T01:five_prime_UTR	30.0%
	AAATTACCACACAAAACTCT+TGG	-	Chr5:22235312-22235331	None:intergenic	30.0%
	AACAAATACCACCGTTATTT+AGG	+	Chr5:22234325-22234344	MsG0580025664.01.T01:intron	30.0%
	AACAATGGAGAGAGAAAAAA+AGG	+	Chr5:22235143-22235162	MsG0580025664.01.T01:intron	30.0%
	AAGCAAAAGAGGTTACATTA+AGG	-	Chr5:22234712-22234731	None:intergenic	30.0%
	ACAACTAATACAATAAGTGG+TGG	-	Chr5:22236006-22236025	None:intergenic	30.0%
	AGACCGAAATAACATAACAA+AGG	+	Chr5:22235649-22235668	MsG0580025664.01.T01:intron	30.0%
	ATATATGTTTGCTTGCGTTA+AGG	+	Chr5:22233623-22233642	MsG0580025664.01.T01:intron	30.0%
	ATCTTATATCCCCTATGAAA+AGG	-	Chr5:22233676-22233695	None:intergenic	30.0%
	ATTAACTAGTGAAGAAATGG+TGG	-	Chr5:22231114-22231133	None:intergenic	30.0%
	ATTACAAATCTTTGGATAGG+AGG	+	Chr5:22233819-22233838	MsG0580025664.01.T01:intron	30.0%
	CAAATTCGTTGTAACAATCT+AGG	-	Chr5:22235431-22235450	None:intergenic	30.0%
	CAACTAATACAATAAGTGGT+GGG	-	Chr5:22236005-22236024	None:intergenic	30.0%
	CAATGACACATTGAATGAAA+TGG	-	Chr5:22236606-22236625	None:intergenic	30.0%
	CACACAACTAATACAATAAG+TGG	-	Chr5:22236009-22236028	None:intergenic	30.0%
	CACTTATTGTATTAGTTGTG+TGG	+	Chr5:22236007-22236026	MsG0580025664.01.T01:intron	30.0%
	CATTTCCTAGATACATTGAA+GGG	-	Chr5:22231809-22231828	None:intergenic	30.0%
	CGTTTAAGAACTCAAATGTA+AGG	+	Chr5:22232651-22232670	MsG0580025664.01.T01:intron	30.0%
	CTCGAAAAACATATGATTTG+TGG	-	Chr5:22233461-22233480	None:intergenic	30.0%
	CTTAAGTCTGTTCTGATTAA+GGG	+	Chr5:22232717-22232736	MsG0580025664.01.T01:intron	30.0%
	CTTCTTATGGTCAAAAACAA+TGG	+	Chr5:22235128-22235147	MsG0580025664.01.T01:intron	30.0%
	GAAATGTGAAAAAGCAAAAG+AGG	-	Chr5:22234723-22234742	None:intergenic	30.0%
	GGCGACAAATTAAATTTCAT+AGG	+	Chr5:22235334-22235353	MsG0580025664.01.T01:intron	30.0%
	GGGACAAAAATAAAGTCAAA+TGG	+	Chr5:22234923-22234942	MsG0580025664.01.T01:intron	30.0%
	GGTCTTTACCATATAAGATT+CGG	+	Chr5:22232347-22232366	MsG0580025664.01.T01:intron	30.0%
	GTATGTGTATGCATATATGT+AGG	-	Chr5:22234584-22234603	None:intergenic	30.0%
	GTTGGAATATGTGTTTATTC+GGG	-	Chr5:22235970-22235989	None:intergenic	30.0%
	GTTTATTTGGATTCAGTTTG+TGG	-	Chr5:22235946-22235965	None:intergenic	30.0%
	TAATGATGTGATGAAAAGTG+AGG	+	Chr5:22234432-22234451	MsG0580025664.01.T01:intron	30.0%
	TAGAAAAAAAAGTCTGCATG+AGG	-	Chr5:22236234-22236253	None:intergenic	30.0%
	TCAAATGGTGGTTAGATATA+GGG	+	Chr5:22234938-22234957	MsG0580025664.01.T01:intron	30.0%
	TCAACAAAGTTGTCATCAAA+TGG	-	Chr5:22235092-22235111	None:intergenic	30.0%
	TCATTTCCTAGATACATTGA+AGG	-	Chr5:22231810-22231829	None:intergenic	30.0%
	TCTGGAAGAATTACAACAAT+TGG	+	Chr5:22234035-22234054	MsG0580025664.01.T01:CDS	30.0%
	TCTTTGAATACTCCAACTTA+AGG	+	Chr5:22231621-22231640	MsG0580025664.01.T01:five_prime_UTR	30.0%
	TGAACTCATGAGAAAATGTT+GGG	+	Chr5:22233584-22233603	MsG0580025664.01.T01:intron	30.0%
	TGTGGGAAAAAAAAAATTGG+GGG	+	Chr5:22232390-22232409	MsG0580025664.01.T01:intron	30.0%
	TGTTTATTCGGGTGTTTATT+TGG	-	Chr5:22235959-22235978	None:intergenic	30.0%
	TTAGATAGAATTGAAGACCT+TGG	-	Chr5:22234100-22234119	None:intergenic	30.0%
	TTGAACTCATGAGAAAATGT+TGG	+	Chr5:22233583-22233602	MsG0580025664.01.T01:intron	30.0%
	TTGAATCATTATGTGTCTTG+TGG	+	Chr5:22233216-22233235	MsG0580025664.01.T01:intron	30.0%
	TTGTTACAACGAATTTGTCA+TGG	+	Chr5:22235434-22235453	MsG0580025664.01.T01:intron	30.0%
	TTTAATGGTAAAAGTCTTGG+AGG	+	Chr5:22236429-22236448	MsG0580025664.01.T01:CDS	30.0%
!	ACATTTCTAAGAAAAGCTGA+CGG	+	Chr5:22234736-22234755	MsG0580025664.01.T01:intron	30.0%
!	ATTTGTCATGGAGAGAATTT+TGG	+	Chr5:22235446-22235465	MsG0580025664.01.T01:intron	30.0%
!	ATTTTTGCAATGAAGCATGA+GGG	+	Chr5:22233094-22233113	MsG0580025664.01.T01:intron	30.0%
!	GAGGGAGTATATACTTAATT+AGG	-	Chr5:22234686-22234705	None:intergenic	30.0%
!	GCTAACTGAGTTTTTCTATT+CGG	-	Chr5:22233400-22233419	None:intergenic	30.0%
!	GCTTTTGTCTCATCTTTTTT+AGG	+	Chr5:22231290-22231309	MsG0580025664.01.T01:five_prime_UTR	30.0%
!	TATAAGATTCGGTACCTAAT+AGG	+	Chr5:22232358-22232377	MsG0580025664.01.T01:intron	30.0%
!	TTTCATTCTGTTGCCTTATT+AGG	+	Chr5:22232921-22232940	MsG0580025664.01.T01:intron	30.0%
!!	AAAAACATCTTTCGAGTTTC+AGG	+	Chr5:22234159-22234178	MsG0580025664.01.T01:intron	30.0%
!!	AAAACATCTTTCGAGTTTCA+GGG	+	Chr5:22234160-22234179	MsG0580025664.01.T01:intron	30.0%
!!	ATTAGGTACCGAATCTTATA+TGG	-	Chr5:22232358-22232377	None:intergenic	30.0%
!!	CTCTAAAATCTGGATATAGA+AGG	-	Chr5:22235523-22235542	None:intergenic	30.0%
!!	GAGTTTTGTGTGGTAATTTT+AGG	+	Chr5:22235313-22235332	MsG0580025664.01.T01:intron	30.0%
!!	TATGTTATTTCGGTCTAGTT+TGG	-	Chr5:22235645-22235664	None:intergenic	30.0%
!!	TATTTTTGCAATGAAGCATG+AGG	+	Chr5:22233093-22233112	MsG0580025664.01.T01:intron	30.0%
!!	TCTATATCCAGATTTTAGAG+AGG	+	Chr5:22235523-22235542	MsG0580025664.01.T01:intron	30.0%
!!!	AACCACTTTTTGACAACTTT+TGG	-	Chr5:22235216-22235235	None:intergenic	30.0%
!!!	ATCATACATGTCGTTTTAGT+CGG	-	Chr5:22236184-22236203	None:intergenic	30.0%
!!!	GTTTTTTTTTGAGGTCATTC+AGG	-	Chr5:22235827-22235846	None:intergenic	30.0%
!!!	TTGTCTCATCTTTTTTAGGT+TGG	+	Chr5:22231294-22231313	MsG0580025664.01.T01:intron	30.0%
!!!	TTTTGGAGCTTTTGTTTGTT+TGG	-	Chr5:22230970-22230989	None:intergenic	30.0%
!!!	TTTTTCGAGTATCCTATGTA+TGG	+	Chr5:22233472-22233491	MsG0580025664.01.T01:intron	30.0%
!!!	TTTTTTTTTTCCCACATTGG+AGG	-	Chr5:22232386-22232405	None:intergenic	30.0%
!!!	TTTTTTTTTTGCGTTACCCT+CGG	-	Chr5:22232063-22232082	None:intergenic	30.0%
	AAATGTAAGGGTCTCTATTG+TGG	+	Chr5:22232664-22232683	MsG0580025664.01.T01:intron	35.0%
	ACTAACAGTTGTCCATACAT+AGG	-	Chr5:22233487-22233506	None:intergenic	35.0%
	AGACCTTGGTTTCAATTACA+CGG	-	Chr5:22234086-22234105	None:intergenic	35.0%
	AGAGCTAACCAATCAAAAGT+TGG	+	Chr5:22233717-22233736	MsG0580025664.01.T01:intron	35.0%
	ATACTCCCTTCAATGTATCT+AGG	+	Chr5:22231801-22231820	MsG0580025664.01.T01:intron	35.0%
	ATAGATGATCTCCATTGAAG+TGG	+	Chr5:22232554-22232573	MsG0580025664.01.T01:intron	35.0%
	ATGTATGGACAACTGTTAGT+GGG	+	Chr5:22233487-22233506	MsG0580025664.01.T01:intron	35.0%
	ATTAGTGTCAGGTCCAAAAA+TGG	-	Chr5:22233027-22233046	None:intergenic	35.0%
	CCATAAGAAGTGAGAAAACA+AGG	-	Chr5:22235118-22235137	None:intergenic	35.0%
	CTCATCATTTCTCTTCTACT+GGG	+	Chr5:22231594-22231613	MsG0580025664.01.T01:five_prime_UTR	35.0%
	GAGAAAGAAAGACCTTAAGT+TGG	-	Chr5:22231636-22231655	None:intergenic	35.0%
	GATCAATGAGCTACAAACAA+AGG	+	Chr5:22234201-22234220	MsG0580025664.01.T01:CDS	35.0%
	GATCACTGTAAAGCCTAATA+AGG	-	Chr5:22232937-22232956	None:intergenic	35.0%
	GCATACACATACACAAATCT+TGG	+	Chr5:22234592-22234611	MsG0580025664.01.T01:intron	35.0%
	GCTTAAGTCTGTTCTGATTA+AGG	+	Chr5:22232716-22232735	MsG0580025664.01.T01:intron	35.0%
	GGTAGACTATCATTATCTCA+CGG	+	Chr5:22231998-22232017	MsG0580025664.01.T01:intron	35.0%
	GTCAAATGGTGGTTAGATAT+AGG	+	Chr5:22234937-22234956	MsG0580025664.01.T01:intron	35.0%
	GTCCAAAAGTTGTCAAAAAG+TGG	+	Chr5:22235211-22235230	MsG0580025664.01.T01:intron	35.0%
	TAGGGGATATAAGATTGACT+TGG	+	Chr5:22233681-22233700	MsG0580025664.01.T01:intron	35.0%
	TATAGAAATTGTGTTGTGGG+TGG	-	Chr5:22231066-22231085	None:intergenic	35.0%
	TATGTATGGACAACTGTTAG+TGG	+	Chr5:22233486-22233505	MsG0580025664.01.T01:intron	35.0%
	TCTCATCATTTCTCTTCTAC+TGG	+	Chr5:22231593-22231612	MsG0580025664.01.T01:five_prime_UTR	35.0%
	TCTCTCTTTCAATTATACGC+TGG	+	Chr5:22231324-22231343	MsG0580025664.01.T01:intron	35.0%
	TTCTATGTCCTAAATAACGG+TGG	-	Chr5:22234336-22234355	None:intergenic	35.0%
	TTTGGACCTGACACTAATTA+GGG	+	Chr5:22233029-22233048	MsG0580025664.01.T01:intron	35.0%
!	GACTTTTCAAAGAGAAGAAG+AGG	+	Chr5:22231519-22231538	MsG0580025664.01.T01:five_prime_UTR	35.0%
!	GCGATCAAGTTATCTTTTGT+AGG	-	Chr5:22233378-22233397	None:intergenic	35.0%
!	TAGTATGATGGTCTTTTTGC+AGG	-	Chr5:22235887-22235906	None:intergenic	35.0%
!	TGCATTGTTCAGACCATTTT+TGG	+	Chr5:22233011-22233030	MsG0580025664.01.T01:intron	35.0%
!	TGGTCTTTTTGCAGGATTTT+TGG	-	Chr5:22235879-22235898	None:intergenic	35.0%
!	TTTATAGGTCTGAAGTGATG+AGG	-	Chr5:22231139-22231158	None:intergenic	35.0%
!	TTTTGGACCTGACACTAATT+AGG	+	Chr5:22233028-22233047	MsG0580025664.01.T01:intron	35.0%
!!	AGTATCCGAGTCTTATTTGA+AGG	+	Chr5:22234976-22234995	MsG0580025664.01.T01:intron	35.0%
!!	ATAAGATTGACTTGGTGGTT+GGG	+	Chr5:22233689-22233708	MsG0580025664.01.T01:intron	35.0%
!!	ATTGGTGTTTTTGATGTACG+AGG	-	Chr5:22235715-22235734	None:intergenic	35.0%
!!	CTCAGAGAAAGCATCAATTA+AGG	+	Chr5:22233980-22233999	MsG0580025664.01.T01:five_prime_UTR	35.0%
!!	TATAAGATTGACTTGGTGGT+TGG	+	Chr5:22233688-22233707	MsG0580025664.01.T01:intron	35.0%
!!	TCAATGTCCTCTCTAAAATC+TGG	-	Chr5:22235533-22235552	None:intergenic	35.0%
!!	TTTGTTTGTTTGGCTTTGTG+AGG	-	Chr5:22230960-22230979	None:intergenic	35.0%
!!!	CCTTGTTTTCTCACTTCTTA+TGG	+	Chr5:22235115-22235134	MsG0580025664.01.T01:intron	35.0%
	AACAACTCTCACTGAACTTG+CGG	+	Chr5:22236131-22236150	MsG0580025664.01.T01:intron	40.0%
	AACTAACCCCAATTTGAGTG+AGG	-	Chr5:22236559-22236578	None:intergenic	40.0%
	AAGGTGTGTTAATGTGTGTC+AGG	-	Chr5:22232853-22232872	None:intergenic	40.0%
	AAGTCTCTTGAAACTGGGTT+GGG	+	Chr5:22234060-22234079	MsG0580025664.01.T01:CDS	40.0%
	AGCATCAATTAAGGCAGATG+AGG	+	Chr5:22233989-22234008	MsG0580025664.01.T01:exon	40.0%
	AGGAGTTGAATATGAGAACG+TGG	+	Chr5:22236449-22236468	MsG0580025664.01.T01:CDS	40.0%
	AGGCTGTGTAAGCAATTCAT+TGG	-	Chr5:22232749-22232768	None:intergenic	40.0%
	AGGTGTGTTAATGTGTGTCA+GGG	-	Chr5:22232852-22232871	None:intergenic	40.0%
	ATGGTGGTTAGATATAGGGA+CGG	+	Chr5:22234942-22234961	MsG0580025664.01.T01:intron	40.0%
	ATTCAGTTTGTGGCAAGTCA+AGG	-	Chr5:22235936-22235955	None:intergenic	40.0%
	ATTCTTCCAGACTCAAACCT+TGG	-	Chr5:22234026-22234045	None:intergenic	40.0%
	CACTCTTTCTCTCTCTTCAT+TGG	+	Chr5:22230913-22230932	MsG0580025664.01.T01:five_prime_UTR	40.0%
	CACTGAAGAAGGTCAATCTT+CGG	+	Chr5:22236473-22236492	MsG0580025664.01.T01:CDS	40.0%
	CACTTGTATGTGTTAGGTGA+CGG	+	Chr5:22236401-22236420	MsG0580025664.01.T01:intron	40.0%
	CATCAATTAAGGCAGATGAG+GGG	+	Chr5:22233991-22234010	MsG0580025664.01.T01:exon	40.0%
	CTAATAGGTACCTCCAATGT+GGG	+	Chr5:22232373-22232392	MsG0580025664.01.T01:intron	40.0%
	GAACTTCCCTAATTAGTGTC+AGG	-	Chr5:22233038-22233057	None:intergenic	40.0%
	GAAGGGTGAATTGTACTTTG+TGG	-	Chr5:22231033-22231052	None:intergenic	40.0%
	GAAGTGGTTCAGTACCTATT+AGG	+	Chr5:22232570-22232589	MsG0580025664.01.T01:intron	40.0%
	GAGAAAAAAAGGAAGAGGAG+AGG	+	Chr5:22235154-22235173	MsG0580025664.01.T01:intron	40.0%
	GCATCAATTAAGGCAGATGA+GGG	+	Chr5:22233990-22234009	MsG0580025664.01.T01:exon	40.0%
	GCGGACGATAGATAAATGTT+TGG	-	Chr5:22235852-22235871	None:intergenic	40.0%
	GGAGAGAGAAAAAAAGGAAG+AGG	+	Chr5:22235149-22235168	MsG0580025664.01.T01:intron	40.0%
	GTCATTCAGGATGAAGTTTG+AGG	-	Chr5:22235814-22235833	None:intergenic	40.0%
	TCAGGGAGAGAAGATTATGA+TGG	+	Chr5:22234177-22234196	MsG0580025664.01.T01:intron	40.0%
	TCGGTCCTTCAAATAAGACT+CGG	-	Chr5:22234984-22235003	None:intergenic	40.0%
	TCTCTGTCTTTCTCTTTCCA+TGG	-	Chr5:22231913-22231932	None:intergenic	40.0%
	TCTTTCTCTCTCTTCATTGG+TGG	+	Chr5:22230916-22230935	MsG0580025664.01.T01:five_prime_UTR	40.0%
	TGAATTGCTTACACAGCCTA+AGG	+	Chr5:22232750-22232769	MsG0580025664.01.T01:intron	40.0%
	TGGAGAAGTCTCTTGAAACT+GGG	+	Chr5:22234055-22234074	MsG0580025664.01.T01:CDS	40.0%
	TTAAGACCCTCAATCTTACG+TGG	+	Chr5:22232436-22232455	MsG0580025664.01.T01:intron	40.0%
	TTAGCTTCTCAAATGTGCGA+GGG	+	Chr5:22233415-22233434	MsG0580025664.01.T01:intron	40.0%
	TTCTTAGGGAAAAGGGACTT+TGG	-	Chr5:22232039-22232058	None:intergenic	40.0%
	TTGGAGAAGTCTCTTGAAAC+TGG	+	Chr5:22234054-22234073	MsG0580025664.01.T01:CDS	40.0%
!	ACTCGAGACCAACTTTTGAT+TGG	-	Chr5:22233728-22233747	None:intergenic	40.0%
!	TTTTTGATGTACGAGGCGTA+TGG	-	Chr5:22235708-22235727	None:intergenic	40.0%
!!	GGGATATAAGATTGACTTGG+TGG	+	Chr5:22233684-22233703	MsG0580025664.01.T01:intron	40.0%
!!	GTGATTCCAAGAGTTTTGTG+TGG	+	Chr5:22235303-22235322	MsG0580025664.01.T01:intron	40.0%
!!	GTTTATGAGAGCATCCACAT+TGG	+	Chr5:22232321-22232340	MsG0580025664.01.T01:intron	40.0%
!!	TAAGATTGACTTGGTGGTTG+GGG	+	Chr5:22233690-22233709	MsG0580025664.01.T01:intron	40.0%
!!	TAGGTACTGAACCACTTCAA+TGG	-	Chr5:22232568-22232587	None:intergenic	40.0%
!!	TAGGTGACGGACATGTTTAA+TGG	+	Chr5:22236414-22236433	MsG0580025664.01.T01:intron	40.0%
!!	TGGAGGTTTGGACTTGTTTT+CGG	-	Chr5:22235760-22235779	None:intergenic	40.0%
!!	TTTGGACTTGTTTTCGGTGT+TGG	-	Chr5:22235754-22235773	None:intergenic	40.0%
!!!	TTGCAGGATTTTTGGTGTAG+CGG	-	Chr5:22235871-22235890	None:intergenic	40.0%
	AAAACCGTCGCTAGATTTGC+AGG	+	Chr5:22233169-22233188	MsG0580025664.01.T01:five_prime_UTR	45.0%
	AAATTGTGTTGTGGGTGGTG+AGG	-	Chr5:22231061-22231080	None:intergenic	45.0%
	AATTGTGTTGTGGGTGGTGA+GGG	-	Chr5:22231060-22231079	None:intergenic	45.0%
	ACAACTCTCACTGAACTTGC+GGG	+	Chr5:22236132-22236151	MsG0580025664.01.T01:intron	45.0%
	AGAGGTTACATTAAGGACGG+AGG	-	Chr5:22234705-22234724	None:intergenic	45.0%
	AGATCTCCAAGGTTTGAGTC+TGG	+	Chr5:22234017-22234036	MsG0580025664.01.T01:CDS	45.0%
	AGGGTCTTAAGTTAGACCCT+GGG	-	Chr5:22232426-22232445	None:intergenic	45.0%
	AGGTGGTTTCTGCTGTTATG+TGG	-	Chr5:22235780-22235799	None:intergenic	45.0%
	ATTATCTCACGGCCAAACTC+TGG	+	Chr5:22232009-22232028	MsG0580025664.01.T01:intron	45.0%
	CCTAATAGGTACCTCCAATG+TGG	+	Chr5:22232372-22232391	MsG0580025664.01.T01:intron	45.0%
	CCTCGGATTCTTAGGGAAAA+GGG	-	Chr5:22232046-22232065	None:intergenic	45.0%
	CTTACACAGCCTAAGGTCTA+GGG	+	Chr5:22232757-22232776	MsG0580025664.01.T01:intron	45.0%
	GAAGTCTCTTGAAACTGGGT+TGG	+	Chr5:22234059-22234078	MsG0580025664.01.T01:CDS	45.0%
	GACTTTGGTCATCCAGAGTT+TGG	-	Chr5:22232024-22232043	None:intergenic	45.0%
	GAGGTTACATTAAGGACGGA+GGG	-	Chr5:22234704-22234723	None:intergenic	45.0%
	GGCTTGAACGAGGTCTTTAT+TGG	-	Chr5:22235733-22235752	None:intergenic	45.0%
	GGGTGAATTGTACTTTGTGG+AGG	-	Chr5:22231030-22231049	None:intergenic	45.0%
	TAACAGCAGAAACCACCTTG+AGG	+	Chr5:22235782-22235801	MsG0580025664.01.T01:intron	45.0%
	TCGGACACCTCACTCAAATT+GGG	+	Chr5:22236549-22236568	MsG0580025664.01.T01:CDS	45.0%
	TCTGCTGTTATGTGGAGGTT+TGG	-	Chr5:22235772-22235791	None:intergenic	45.0%
	TGGTAAAGACCCTACCAATG+TGG	-	Chr5:22232338-22232357	None:intergenic	45.0%
	TGGTTAGATATAGGGACGGA+GGG	+	Chr5:22234946-22234965	MsG0580025664.01.T01:intron	45.0%
	TGGTTTCTGCTGTTATGTGG+AGG	-	Chr5:22235777-22235796	None:intergenic	45.0%
	TTCTTAAACGGTTCCCCCAT+GGG	-	Chr5:22232641-22232660	None:intergenic	45.0%
!	ATTGTACTTTGTGGAGGCGT+TGG	-	Chr5:22231024-22231043	None:intergenic	45.0%
!	CAACTGTTCTAGACTGAGCA+AGG	+	Chr5:22233952-22233971	MsG0580025664.01.T01:five_prime_UTR	45.0%
!	CCTTTTCCCTAAGAATCCGA+GGG	+	Chr5:22232044-22232063	MsG0580025664.01.T01:intron	45.0%
!	GGTACCACTGAAGTATCTGT+GGG	+	Chr5:22233328-22233347	MsG0580025664.01.T01:intron	45.0%
!	GTTAGCTTCTCAAATGTGCG+AGG	+	Chr5:22233414-22233433	MsG0580025664.01.T01:intron	45.0%
!	TGGTACCACTGAAGTATCTG+TGG	+	Chr5:22233327-22233346	MsG0580025664.01.T01:intron	45.0%
!	TTTTCCAATCGCTCCGTGTT+TGG	-	Chr5:22232787-22232806	None:intergenic	45.0%
!!	AGTTTGAGGCTTTTGCCTCA+AGG	-	Chr5:22235800-22235819	None:intergenic	45.0%
!!	ATGAGAGCATCCACATTGGT+AGG	+	Chr5:22232325-22232344	MsG0580025664.01.T01:intron	45.0%
!!	CCACATTGGAGGTACCTATT+AGG	-	Chr5:22232375-22232394	None:intergenic	45.0%
!!	TGAGAGCATCCACATTGGTA+GGG	+	Chr5:22232326-22232345	MsG0580025664.01.T01:intron	45.0%
!!	TTTGTGGAGGCGTTGGTATT+TGG	-	Chr5:22231017-22231036	None:intergenic	45.0%
!!	AAAAAAGAATAAAATTTAAT+AGG	-	Chr5:22234652-22234671	None:intergenic	5.0%
	ACTTGCGGGAAGGCAAATGA+TGG	+	Chr5:22236146-22236165	MsG0580025664.01.T01:intron	50.0%
	AGATTGAAAACGCAACGGCG+AGG	+	Chr5:22231491-22231510	MsG0580025664.01.T01:five_prime_UTR	50.0%
	ATGAGGGGAGAAGATCTCCA+AGG	+	Chr5:22234006-22234025	MsG0580025664.01.T01:CDS	50.0%
	ATGTGTACATGTGGGACCCA+TGG	+	Chr5:22232622-22232641	MsG0580025664.01.T01:intron	50.0%
	CAACTCTACTAGTCCTCCAC+AGG	+	Chr5:22236515-22236534	MsG0580025664.01.T01:CDS	50.0%
	CATGCCCACAGATACTTCAG+TGG	-	Chr5:22233335-22233354	None:intergenic	50.0%
	CCAACCTGCAAATCTAGCGA+CGG	-	Chr5:22233176-22233195	None:intergenic	50.0%
	CCCTCGGATTCTTAGGGAAA+AGG	-	Chr5:22232047-22232066	None:intergenic	50.0%
	CGGACACCTCACTCAAATTG+GGG	+	Chr5:22236550-22236569	MsG0580025664.01.T01:CDS	50.0%
	CTCGGACACCTCACTCAAAT+TGG	+	Chr5:22236548-22236567	MsG0580025664.01.T01:CDS	50.0%
	GAGAACGTGGTCACTGAAGA+AGG	+	Chr5:22236462-22236481	MsG0580025664.01.T01:CDS	50.0%
	GAGGGTCTTAAGTTAGACCC+TGG	-	Chr5:22232427-22232446	None:intergenic	50.0%
	GCGTTACCCTCGGATTCTTA+GGG	-	Chr5:22232053-22232072	None:intergenic	50.0%
	GCTTACACAGCCTAAGGTCT+AGG	+	Chr5:22232756-22232775	MsG0580025664.01.T01:intron	50.0%
	GGGCCGTGTAATTGAAACCA+AGG	+	Chr5:22234080-22234099	MsG0580025664.01.T01:CDS	50.0%
	GGTGGGATCTGAATGAGTGT+TGG	-	Chr5:22235988-22236007	None:intergenic	50.0%
	GTGGTTAGATATAGGGACGG+AGG	+	Chr5:22234945-22234964	MsG0580025664.01.T01:intron	50.0%
	GTTCTTAAACGGTTCCCCCA+TGG	-	Chr5:22232642-22232661	None:intergenic	50.0%
	TCCACAGGACTATGAGAGCT+CGG	+	Chr5:22236530-22236549	MsG0580025664.01.T01:CDS	50.0%
	TGCGTTACCCTCGGATTCTT+AGG	-	Chr5:22232054-22232073	None:intergenic	50.0%
	TGGGGTCCACGTAAGATTGA+GGG	-	Chr5:22232445-22232464	None:intergenic	50.0%
	TGTGTACATGTGGGACCCAT+GGG	+	Chr5:22232623-22232642	MsG0580025664.01.T01:intron	50.0%
!	CACTGAAGTATCTGTGGGCA+TGG	+	Chr5:22233333-22233352	MsG0580025664.01.T01:intron	50.0%
!	CCCTTTTCCCTAAGAATCCG+AGG	+	Chr5:22232043-22232062	MsG0580025664.01.T01:intron	50.0%
!	TTGAGGCTTTTGCCTCAAGG+TGG	-	Chr5:22235797-22235816	None:intergenic	50.0%
!	TTTGGTGGTCCCTAGACCTT+AGG	-	Chr5:22232769-22232788	None:intergenic	50.0%
!!	TTTCGGTGTTGGCTTGAACG+AGG	-	Chr5:22235743-22235762	None:intergenic	50.0%
	ACCACCAAACACGGAGCGAT+TGG	+	Chr5:22232780-22232799	MsG0580025664.01.T01:intron	55.0%
	CCGTCGCTAGATTTGCAGGT+TGG	+	Chr5:22233173-22233192	MsG0580025664.01.T01:intron	55.0%
	CTCTCACTGAACTTGCGGGA+AGG	+	Chr5:22236136-22236155	MsG0580025664.01.T01:intron	55.0%
	GAGCTCTCATAGTCCTGTGG+AGG	-	Chr5:22236531-22236550	None:intergenic	55.0%
	GCTTCTCAAATGTGCGAGGG+TGG	+	Chr5:22233418-22233437	MsG0580025664.01.T01:intron	55.0%
	GGTCTAGGGACCACCAAACA+CGG	+	Chr5:22232771-22232790	MsG0580025664.01.T01:intron	55.0%
	GTGGGGTCCACGTAAGATTG+AGG	-	Chr5:22232446-22232465	None:intergenic	55.0%
	GTGTACATGTGGGACCCATG+GGG	+	Chr5:22232624-22232643	MsG0580025664.01.T01:intron	55.0%
	TCCAATCGCTCCGTGTTTGG+TGG	-	Chr5:22232784-22232803	None:intergenic	55.0%
	TCCGAGCTCTCATAGTCCTG+TGG	-	Chr5:22236534-22236553	None:intergenic	55.0%
	TGGGGGTCTCTTAAGACCCA+GGG	+	Chr5:22232407-22232426	MsG0580025664.01.T01:intron	55.0%
	TGGGTGGTGAGGGTTTGGAA+GGG	-	Chr5:22231050-22231069	None:intergenic	55.0%
	TGTACATGTGGGACCCATGG+GGG	+	Chr5:22232625-22232644	MsG0580025664.01.T01:intron	55.0%
	TGTTGTGGGTGGTGAGGGTT+TGG	-	Chr5:22231055-22231074	None:intergenic	55.0%
	TTGGGGGTCTCTTAAGACCC+AGG	+	Chr5:22232406-22232425	MsG0580025664.01.T01:intron	55.0%
	GTGGGTGGTGAGGGTTTGGA+AGG	-	Chr5:22231051-22231070	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	22230906	22236632	22230906	ID=MsG0580025664.01;Name=MsG0580025664.01
Chr5	mRNA	22230906	22236632	22230906	ID=MsG0580025664.01.T01;Parent=MsG0580025664.01;Name=MsG0580025664.01.T01;_AED=0.31;_eAED=0.35;_QI=761\|0.8\|0.83\|1\|0\|0\|6\|0\|117
Chr5	exon	22230906	22231311	22230906	ID=MsG0580025664.01.T01:exon:23854;Parent=MsG0580025664.01.T01
Chr5	exon	22231438	22231642	22231438	ID=MsG0580025664.01.T01:exon:23855;Parent=MsG0580025664.01.T01
Chr5	exon	22233109	22233190	22233109	ID=MsG0580025664.01.T01:exon:23856;Parent=MsG0580025664.01.T01
Chr5	exon	22233938	22234101	22233938	ID=MsG0580025664.01.T01:exon:23857;Parent=MsG0580025664.01.T01
Chr5	exon	22234181	22234222	22234181	ID=MsG0580025664.01.T01:exon:23858;Parent=MsG0580025664.01.T01
Chr5	exon	22236417	22236632	22236417	ID=MsG0580025664.01.T01:exon:23859;Parent=MsG0580025664.01.T01
Chr5	five_prime_UTR	22230906	22231311	22230906	ID=MsG0580025664.01.T01:five_prime_utr;Parent=MsG0580025664.01.T01
Chr5	five_prime_UTR	22231438	22231642	22231438	ID=MsG0580025664.01.T01:five_prime_utr;Parent=MsG0580025664.01.T01
Chr5	five_prime_UTR	22233109	22233190	22233109	ID=MsG0580025664.01.T01:five_prime_utr;Parent=MsG0580025664.01.T01
Chr5	five_prime_UTR	22233938	22234005	22233938	ID=MsG0580025664.01.T01:five_prime_utr;Parent=MsG0580025664.01.T01
Chr5	CDS	22234006	22234101	22234006	ID=MsG0580025664.01.T01:cds;Parent=MsG0580025664.01.T01
Chr5	CDS	22234181	22234222	22234181	ID=MsG0580025664.01.T01:cds;Parent=MsG0580025664.01.T01
Chr5	CDS	22236417	22236632	22236417	ID=MsG0580025664.01.T01:cds;Parent=MsG0580025664.01.T01

Gene Sequence	>MsG0580025664.01.T01 ATGAGGGGAGAAGATCTCCAAGGTTTGAGTCTGGAAGAATTACAACAATTGGAGAAGTCTCTTGAAACTGGGTTGGGCCGTGTAATTGAAACCAAGGGAGAGAAGATTATGATGGAGATCAATGAGCTACAAACAAAGGTGACGGACATGTTTAATGGTAAAAGTCTTGGAGGAGTTGAATATGAGAACGTGGTCACTGAAGAAGGTCAATCTTCGGAATCAATCACTAATGTTTACAACTCTACTAGTCCTCCACAGGACTATGAGAGCTCGGACACCTCACTCAAATTGGGGTTAGTTCACTTTGATTCACACACATTTATACCATTTCATTCAATGTGTCATTGCATATAA
Protein sequence	>MsG0580025664.01.T01 MRGEDLQGLSLEELQQLEKSLETGLGRVIETKGEKIMMEINELQTKVTDMFNGKSLGGVEYENVVTEEGQSSESITNVYNSTSPPQDYESSDTSLKLGLVHFDSHTFIPFHSMCHCI*