Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1364110 | XP_003597562.1 | 99.758 | 413 | 1 | 0 | 1 | 413 | 1 | 413 | 0.0 | 850 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1364110 | sp|Q40465|IF411_TOBAC | 93.947 | 413 | 25 | 0 | 1 | 413 | 1 | 413 | 0.0 | 802 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0011060 | Msa1364110 | -0.801990 | 6.935386e-49 | -8.615850e-47 |
| Msa0016710 | Msa1364110 | 0.804960 | 1.680399e-49 | -8.615850e-47 |
| Msa0021290 | Msa1364110 | 0.827083 | 1.902978e-54 | -8.615850e-47 |
| Msa0021320 | Msa1364110 | 0.833588 | 4.892720e-56 | -8.615850e-47 |
| Msa0027590 | Msa1364110 | 0.806671 | 7.345920e-50 | -8.615850e-47 |
| Msa0037180 | Msa1364110 | 0.827577 | 1.449153e-54 | -8.615850e-47 |
| Msa0037650 | Msa1364110 | -0.812992 | 3.206700e-51 | -8.615850e-47 |
| Msa0040220 | Msa1364110 | 0.811957 | 5.398920e-51 | -8.615850e-47 |
| Msa0044010 | Msa1364110 | 0.864063 | 1.591780e-64 | -8.615850e-47 |
| Msa0066680 | Msa1364110 | -0.810335 | 1.213432e-50 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1364110 | MtrunA17_Chr2g0329931 | 99.758 | 413 | 1 | 0 | 1 | 413 | 1 | 413 | 0.0 | 850 |
| Msa1364110 | MtrunA17_Chr8g0342861 | 94.189 | 413 | 24 | 0 | 1 | 413 | 1 | 413 | 0.0 | 808 |
| Msa1364110 | MtrunA17_Chr2g0299681 | 92.978 | 413 | 29 | 0 | 1 | 413 | 1 | 413 | 0.0 | 800 |
| Msa1364110 | MtrunA17_Chr4g0070981 | 91.525 | 413 | 35 | 0 | 1 | 413 | 1 | 413 | 0.0 | 781 |
| Msa1364110 | MtrunA17_Chr2g0297261 | 64.611 | 373 | 132 | 0 | 41 | 413 | 34 | 406 | 0.0 | 517 |
| Msa1364110 | MtrunA17_Chr1g0169331 | 61.168 | 394 | 116 | 5 | 56 | 413 | 6 | 398 | 3.37e-164 | 467 |
| Msa1364110 | MtrunA17_Chr8g0342851 | 63.584 | 346 | 94 | 4 | 71 | 413 | 3 | 319 | 8.18e-145 | 414 |
| Msa1364110 | MtrunA17_Chr7g0247181 | 40.984 | 366 | 212 | 4 | 42 | 406 | 141 | 503 | 7.04e-89 | 278 |
| Msa1364110 | MtrunA17_Chr7g0247191 | 41.257 | 366 | 211 | 4 | 42 | 406 | 151 | 513 | 8.71e-89 | 278 |
| Msa1364110 | MtrunA17_Chr7g0247221 | 40.984 | 366 | 212 | 4 | 42 | 406 | 144 | 506 | 1.05e-88 | 278 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1364110 | AT1G54270.1 | 92.995 | 414 | 26 | 2 | 1 | 413 | 1 | 412 | 0.0 | 792 |
| Msa1364110 | AT3G13920.1 | 92.512 | 414 | 28 | 2 | 1 | 413 | 1 | 412 | 0.0 | 788 |
| Msa1364110 | AT1G54270.2 | 91.787 | 414 | 26 | 3 | 1 | 413 | 1 | 407 | 0.0 | 775 |
| Msa1364110 | AT3G13920.4 | 91.304 | 414 | 28 | 3 | 1 | 413 | 1 | 407 | 0.0 | 770 |
| Msa1364110 | AT1G72730.1 | 89.372 | 414 | 43 | 1 | 1 | 413 | 1 | 414 | 0.0 | 769 |
| Msa1364110 | AT3G13920.3 | 94.330 | 388 | 21 | 1 | 27 | 413 | 15 | 402 | 0.0 | 755 |
| Msa1364110 | AT3G13920.2 | 92.366 | 393 | 27 | 2 | 1 | 392 | 1 | 391 | 0.0 | 746 |
| Msa1364110 | AT3G13920.5 | 92.366 | 393 | 27 | 2 | 1 | 392 | 5 | 395 | 0.0 | 745 |
| Msa1364110 | AT3G19760.1 | 62.725 | 389 | 138 | 1 | 25 | 413 | 27 | 408 | 0.0 | 511 |
| Msa1364110 | AT1G51380.1 | 58.108 | 370 | 155 | 0 | 40 | 409 | 22 | 391 | 1.33e-162 | 462 |
Find 118 sgRNAs with CRISPR-Local
Find 213 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACTATCTCCATCGTATT+GGG | 0.393330 | tig0018106:-181816 | Msa1364110:CDS |
| AAATAAGGGTTGCTAGCATA+AGG | 0.415054 | tig0018106:+181432 | None:intergenic |
| AACTCCACGGCGCAGATCCG+AGG | 0.568103 | tig0018106:-184548 | Msa1364110:five_prime_UTR |
| AACTCCTCGGATCTGCGCCG+TGG | 0.597296 | tig0018106:+184544 | None:intergenic |
| AAGACAGCGACGTTCTGTTC+TGG | 0.339543 | tig0018106:-182883 | Msa1364110:CDS |
| AAGCATGAACCTTAACACCA+AGG | 0.715621 | tig0018106:+182749 | None:intergenic |
| AAGGTTCATGCTTGTGTTGG+TGG | 0.466235 | tig0018106:-182739 | Msa1364110:CDS |
| AATGCTTTCACGTGGTTTCA+AGG | 0.432515 | tig0018106:-182572 | Msa1364110:CDS |
| AATTACAGGCTTTCCACTGA+AGG | 0.540134 | tig0018106:-183650 | Msa1364110:intron |
| ACACAAGGCGCAAGGTGGAC+TGG | 0.505122 | tig0018106:-182038 | Msa1364110:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GTATGTATTCTACTACTACT+AGG | + | tig0018106:181385-181404 | None:intergenic | 30.0% | |
| CAAGAAAACACATAACACAA+GGG | + | tig0018106:182094-182113 | None:intergenic | 30.0% | |
| AGAACAAAACAGTACACTTT+AGG | + | tig0018106:182198-182217 | None:intergenic | 30.0% | |
| TTGATAGAAAATCTTCTGAG+AGG | - | tig0018106:182441-182460 | Msa1364110:intron | 30.0% | |
| AATTGTGATAGGCACTAATT+GGG | + | tig0018106:182562-182581 | None:intergenic | 30.0% | |
| AAATTGTGATAGGCACTAAT+TGG | + | tig0018106:182563-182582 | None:intergenic | 30.0% | |
| ATATAGTGACAACTATCAAG+GGG | + | tig0018106:182736-182755 | None:intergenic | 30.0% | |
| CTATATAGTGACAACTATCA+AGG | + | tig0018106:182738-182757 | None:intergenic | 30.0% | |
| TATATACCAATGACAAGCAT+GGG | + | tig0018106:182762-182781 | None:intergenic | 30.0% | |
| ATATATACCAATGACAAGCA+TGG | + | tig0018106:182763-182782 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0018106 | gene | 181376 | 184659 | 181376 | ID=Msa1364110;Name=Msa1364110 |
| tig0018106 | mRNA | 181376 | 184659 | 181376 | ID=Msa1364110-mRNA-1;Parent=Msa1364110;Name=Msa1364110-mRNA-1;_AED=0.05;_eAED=0.05;_QI=184|1|1|1|0.75|0.6|5|302|413 |
| tig0018106 | exon | 181376 | 182313 | 181376 | ID=Msa1364110-mRNA-1:exon:13866;Parent=Msa1364110-mRNA-1 |
| tig0018106 | exon | 182567 | 182997 | 182567 | ID=Msa1364110-mRNA-1:exon:13865;Parent=Msa1364110-mRNA-1 |
| tig0018106 | exon | 183558 | 183664 | 183558 | ID=Msa1364110-mRNA-1:exon:13864;Parent=Msa1364110-mRNA-1 |
| tig0018106 | exon | 183744 | 183813 | 183744 | ID=Msa1364110-mRNA-1:exon:13863;Parent=Msa1364110-mRNA-1 |
| tig0018106 | exon | 184478 | 184659 | 184478 | ID=Msa1364110-mRNA-1:exon:13862;Parent=Msa1364110-mRNA-1 |
| tig0018106 | five_prime_UTR | 184478 | 184659 | 184478 | ID=Msa1364110-mRNA-1:five_prime_utr;Parent=Msa1364110-mRNA-1 |
| tig0018106 | five_prime_UTR | 183812 | 183813 | 183812 | ID=Msa1364110-mRNA-1:five_prime_utr;Parent=Msa1364110-mRNA-1 |
| tig0018106 | CDS | 183744 | 183811 | 183744 | ID=Msa1364110-mRNA-1:cds;Parent=Msa1364110-mRNA-1 |
| tig0018106 | CDS | 183558 | 183664 | 183558 | ID=Msa1364110-mRNA-1:cds;Parent=Msa1364110-mRNA-1 |
| tig0018106 | CDS | 182567 | 182997 | 182567 | ID=Msa1364110-mRNA-1:cds;Parent=Msa1364110-mRNA-1 |
| tig0018106 | CDS | 181678 | 182313 | 181678 | ID=Msa1364110-mRNA-1:cds;Parent=Msa1364110-mRNA-1 |
| tig0018106 | three_prime_UTR | 181376 | 181677 | 181376 | ID=Msa1364110-mRNA-1:three_prime_utr;Parent=Msa1364110-mRNA-1 |
| Gene Sequence |
>Msa1364110 ATGGCAGGTTTAGCTCCGGAAGGTTCACAATTTGATACCAAGCAGTTTGACACTAAGATGAATGAATTGCTTTCCACTGAAGGACAAGAATTCTTCACCTCATACGATGAAGTTCATGAAACTTTTGATGCAATGGGATTGATAGAAAATCTTCTGAGAGGCATATATGCTTATGGTTTTGAGAAGCCATCTGCAATACAGCAAAGGGGAATTGTTCCTTTTATCAAGGGTCTCGATGTGATTCAGCAGGCTCAGTCAGGAACTGGAAAGACAGCGACGTTCTGTTCTGGAATTTTGCAGCAGCTTGATTATGGTTTGGTTCAGTGCCAGGCTTTGGTTTTGGCCCCAACAAGGGAGCTGGCACAGCAGATTGAGAAGGTTATGAGAGCACTTGGTGATTACCTTGGTGTTAAGGTTCATGCTTGTGTTGGTGGCACTAGTGTTCGTGAGGATCAGCGTATTCTCCAAACTGGTGTCCATGTTGTTGTTGGTACTCCTGGACGTGTGTTTGACATGCTGCGAAGACAGTCTCTTCGGTCAGATTGCATTAAAATGTTTGTTTTGGATGAAGCTGATGAAATGCTTTCACGTGGTTTCAAGGACCAGATCTACGACATCTTCCAGTTGCTGCCACCCAAAATTCAGGTTGGAGTGTTCTCTGCTACAATGCCTCCAGAGGCTCTGGAGATCACAAGGAAGTTCATGAATAAGCCAGTTAGAATTCTGGTAAAGCGTGATGAATTGACCCTTGAGGGTATCAAGCAGTTCTACGTGAATGTTGACAAGGAAGAGTGGAAACTAGAGACATTGTGTGATCTTTATGAGACTCTGGCCATCACTCAGAGTGTCATTTTTGTGAACACAAGGCGCAAGGTGGACTGGCTCACTGACAAGATGCGAGGCAGAGACCATACTGTCTCAGCCACCCATGGAGACATGGACCAAAACACTCGTGATATCATCATGCGTGAATTCCGCTCTGGCTCTTCTCGAGTTCTCATTACCACTGACCTTTTGGCCCGTGGTATTGATGTTCAGCAAGTGTCTCTGGTCATAAATTATGATTTGCCTACCCAACCTGAAAACTATCTCCATCGTATTGGGCGAAGTGGGCGTTTTGGAAGAAAAGGTGTTGCTATCAACTTCGTCACATTGGAAGATGCAAGAATGCTGGCTGATATTCAGAAGTTCTATAATGTGATCGTAGAGGAACTGCCTTCAAATGTTGCTGATCTACTCTGA |
| Protein sequence |
>Msa1364110 MAGLAPEGSQFDTKQFDTKMNELLSTEGQEFFTSYDEVHETFDAMGLIENLLRGIYAYGFEKPSAIQQRGIVPFIKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQTGVHVVVGTPGRVFDMLRRQSLRSDCIKMFVLDEADEMLSRGFKDQIYDIFQLLPPKIQVGVFSATMPPEALEITRKFMNKPVRILVKRDELTLEGIKQFYVNVDKEEWKLETLCDLYETLAITQSVIFVNTRRKVDWLTDKMRGRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTLEDARMLADIQKFYNVIVEELPSNVADLL* |
