AlfalfaGEDB | Msa0430780

M. sativa cultivar zhongmu-4 / Msa0430780

KEGG: K22419
GO: GO:0003002 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006629 GO:0006950 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0008202 GO:0009611 GO:0010051 GO:0016491 GO:0016627 GO:0016628 GO:0032501 GO:0032502 GO:0035671 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0048856 GO:0050896 GO:0055114 GO:0071704 GO:1901360
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0430780	RHN70349.1	96.104	77	2	1	1	76	302	378	1.33e-42	152

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0430780	sp\|A0A221J5W8\|ISY1_NEPCA	64.935	77	26	1	1	76	312	388	1.00e-28	108

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0430780	A0A396IXB6	96.104	77	2	1	1	76	302	378	6.34e-43	152

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0014800	Msa0430780	0.815629	8.381334e-52	-8.615850e-47
Msa0015300	Msa0430780	0.807287	5.440937e-50	-8.615850e-47
Msa0017510	Msa0430780	0.812020	5.229941e-51	-8.615850e-47
Msa0037700	Msa0430780	0.810474	1.131931e-50	-8.615850e-47
Msa0067620	Msa0430780	0.803213	3.880449e-49	-8.615850e-47
Msa0091570	Msa0430780	0.806895	6.585859e-50	-8.615850e-47
Msa0092990	Msa0430780	0.805470	1.314440e-49	-8.615850e-47
Msa0113750	Msa0430780	0.802972	4.352041e-49	-8.615850e-47
Msa0157290	Msa0430780	0.811813	5.802298e-51	-8.615850e-47
Msa0398950	Msa0430780	0.805037	1.619570e-49	-8.615850e-47
Msa0430780	Msa0442200	0.804111	2.526353e-49	-8.615850e-47
Msa0430780	Msa0484780	0.801378	9.264531e-49	-8.615850e-47
Msa0430780	Msa0553220	-0.817069	3.991223e-52	-8.615850e-47
Msa0430780	Msa0608160	0.817396	3.370103e-52	-8.615850e-47
Msa0430780	Msa0631200	0.814948	1.187711e-51	-8.615850e-47
Msa0430780	Msa0647450	-0.802263	6.095365e-49	-8.615850e-47
Msa0430780	Msa0648080	0.819624	1.053173e-52	-8.615850e-47
Msa0430780	Msa0663920	0.803333	3.665234e-49	-8.615850e-47
Msa0430780	Msa0709500	0.822602	2.170249e-53	-8.615850e-47
Msa0430780	Msa0722020	0.811009	8.674557e-51	-8.615850e-47
Msa0430780	Msa0743720	0.808589	2.875253e-50	-8.615850e-47
Msa0430780	Msa0763020	0.821343	4.247619e-53	-8.615850e-47
Msa0430780	Msa0763070	0.818572	1.828073e-52	-8.615850e-47
Msa0430780	Msa0771720	0.802282	6.041265e-49	-8.615850e-47
Msa0430780	Msa0790850	0.817614	3.010179e-52	-8.615850e-47
Msa0430780	Msa0824680	0.809176	2.153888e-50	-8.615850e-47
Msa0430780	Msa0847080	0.814794	1.285056e-51	-8.615850e-47
Msa0430780	Msa0874500	0.801915	7.188671e-49	-8.615850e-47
Msa0430780	Msa0877410	0.811538	6.659399e-51	-8.615850e-47
Msa0430780	Msa0880140	0.810657	1.033473e-50	-8.615850e-47
Msa0430780	Msa0899230	0.801940	7.103214e-49	-8.615850e-47
Msa0430780	Msa0927670	0.806949	6.415429e-50	-8.615850e-47
Msa0430780	Msa0970030	0.801284	9.681393e-49	-8.615850e-47
Msa0430780	Msa1013040	0.803889	2.809565e-49	-8.615850e-47
Msa0430780	Msa1040400	0.818804	1.619395e-52	-8.615850e-47
Msa0430780	Msa1060220	0.816167	6.356723e-52	-8.615850e-47
Msa0430780	Msa1097840	0.804940	1.696756e-49	-8.615850e-47
Msa0430780	Msa1125760	0.818125	2.307830e-52	-8.615850e-47
Msa0430780	Msa1143930	-0.807667	4.520017e-50	-8.615850e-47
Msa0430780	Msa1171360	0.821583	3.737923e-53	-8.615850e-47
Msa0430780	Msa1171870	0.810976	8.815830e-51	-8.615850e-47
Msa0430780	Msa1174040	0.811215	7.826745e-51	-8.615850e-47
Msa0430780	Msa1190930	0.810805	9.600243e-51	-8.615850e-47
Msa0430780	Msa1204250	0.808381	3.185588e-50	-8.615850e-47
Msa0430780	Msa1218600	0.800071	1.711245e-48	-8.615850e-47
Msa0430780	Msa1247250	0.826138	3.197660e-54	-8.615850e-47
Msa0430780	Msa1252140	0.806717	7.181438e-50	-8.615850e-47
Msa0430780	Msa1275360	0.811980	5.335318e-51	-8.615850e-47
Msa0430780	Msa1302190	-0.805315	1.416578e-49	-8.615850e-47
Msa0430780	Msa1305340	0.818076	2.366728e-52	-8.615850e-47
Msa0430780	Msa1306430	0.815593	8.540278e-52	-8.615850e-47
Msa0430780	Msa1333110	0.808859	2.518350e-50	-8.615850e-47
Msa0430780	Msa1348950	0.803179	3.943943e-49	-8.615850e-47
Msa0430780	Msa1356440	0.801004	1.104740e-48	-8.615850e-47
Msa0430780	Msa1382410	0.804023	2.635558e-49	-8.615850e-47
Msa0430780	Msa1461280	0.831327	1.777876e-55	-8.615850e-47
Msa0242230	Msa0430780	0.803044	4.206903e-49	-8.615850e-47
Msa0269450	Msa0430780	0.809829	1.559278e-50	-8.615850e-47
Msa0291500	Msa0430780	0.800545	1.370834e-48	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0430780	MtrunA17_Chr3g0134571	96.104	77	2	1	1	76	302	378	1.22e-46	152
Msa0430780	MtrunA17_Chr3g0134551	80.519	77	14	1	1	76	314	390	2.17e-37	128
Msa0430780	MtrunA17_Chr3g0082001	80.519	77	14	1	1	76	314	390	2.31e-37	128
Msa0430780	MtrunA17_Chr3g0134561	74.026	77	19	1	1	76	314	390	7.30e-35	121
Msa0430780	MtrunA17_Chr1g0152791	68.831	77	23	1	1	76	131	207	3.39e-32	110
Msa0430780	MtrunA17_Chr1g0152811	70.130	77	22	1	1	76	349	425	7.18e-32	114
Msa0430780	MtrunA17_Chr1g0152831	70.667	75	21	1	1	74	347	421	2.68e-30	110
Msa0430780	MtrunA17_Chr4g0036961	41.026	78	44	1	1	76	295	372	2.19e-13	63.2
Msa0430780	MtrunA17_Chr4g0068121	41.026	78	44	1	1	76	295	372	7.55e-13	61.6
Msa0430780	MtrunA17_Chr4g0068141	39.744	78	45	1	1	76	295	372	3.30e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0430780	AT4G24220.1	64.935	77	26	1	1	76	312	388	7.99e-27	100
Msa0430780	AT4G24220.2	64.935	77	26	1	1	76	311	387	8.07e-27	100

Find 19 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCAAGAATTCGCTCGTAAAT+TGG	0.252485	3_2:+82662505	Msa0430780:CDS
TGGGTTGCGGATGCTACTTT+TGG	0.386076	3_2:+82662416	Msa0430780:CDS
TTCACAATCTCATCCCAAAC+TGG	0.408919	3_2:-82662347	None:intergenic
TGAAGGATAAGGGTCCAGTT+TGG	0.410394	3_2:+82662333	Msa0430780:CDS
GAAGGATAAGGGTCCAGTTT+GGG	0.418277	3_2:+82662334	Msa0430780:CDS
GGCTGAGATGATGAAGGATA+AGG	0.462501	3_2:+82662322	None:intergenic
CGGAACAATCTTGTAAGCTC+TGG	0.510489	3_2:-82662538	None:intergenic
GAGGTTGGCTGAGATGATGA+AGG	0.510652	3_2:+82662316	None:intergenic
ATGAACAAGGCGAAAGAGCA+TGG	0.531720	3_2:+82662462	Msa0430780:CDS
CGAGCTTCAACCTACGAAGT+TGG	0.543868	3_2:+82662376	Msa0430780:CDS
TGGAAGAAGTTGCTGAGTGG+TGG	0.544125	3_2:+82662396	Msa0430780:CDS
AGTTGCTGAGTGGTGGGTTG+CGG	0.546335	3_2:+82662403	Msa0430780:CDS
GCTGAGATGATGAAGGATAA+GGG	0.554744	3_2:+82662323	None:intergenic
GCAACTTCTTCCAACTTCGT+AGG	0.555598	3_2:-82662386	None:intergenic
GGAAGAAGTTGCTGAGTGGT+GGG	0.573473	3_2:+82662397	Msa0430780:CDS
TGAACAAGGCGAAAGAGCAT+GGG	0.609225	3_2:+82662463	Msa0430780:CDS
AGTTTGGGATGAGATTGTGA+AGG	0.658349	3_2:+82662349	Msa0430780:CDS
ATTTGTAGATAGCATGAACA+AGG	0.669774	3_2:+82662449	Msa0430780:intron
AGTTGGAAGAAGTTGCTGAG+TGG	0.765321	3_2:+82662393	Msa0430780:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATTTGTAGATAGCATGAACA+AGG	+	chr3_2:82662449-82662468	Msa0430780:intron	30.0%
	TTCACAATCTCATCCCAAAC+TGG	-	chr3_2:82662350-82662369	None:intergenic	40.0%
!	AGTTTGGGATGAGATTGTGA+AGG	+	chr3_2:82662349-82662368	Msa0430780:CDS	40.0%
	GCAAGAATTCGCTCGTAAAT+TGG	+	chr3_2:82662505-82662524	Msa0430780:CDS	40.0%
	TGAAGGATAAGGGTCCAGTT+TGG	+	chr3_2:82662333-82662352	Msa0430780:CDS	45.0%
	GAAGGATAAGGGTCCAGTTT+GGG	+	chr3_2:82662334-82662353	Msa0430780:CDS	45.0%
	GCAACTTCTTCCAACTTCGT+AGG	-	chr3_2:82662389-82662408	None:intergenic	45.0%
	AGTTGGAAGAAGTTGCTGAG+TGG	+	chr3_2:82662393-82662412	Msa0430780:CDS	45.0%
!!!	TGCGGATGCTACTTTTGGAA+TGG	+	chr3_2:82662421-82662440	Msa0430780:CDS	45.0%
	ATGAACAAGGCGAAAGAGCA+TGG	+	chr3_2:82662462-82662481	Msa0430780:CDS	45.0%
!	TGAACAAGGCGAAAGAGCAT+GGG	+	chr3_2:82662463-82662482	Msa0430780:CDS	45.0%
!!	GCGAAAGAGCATGGGTTTTT+GGG	+	chr3_2:82662471-82662490	Msa0430780:CDS	45.0%
	CGGAACAATCTTGTAAGCTC+TGG	-	chr3_2:82662541-82662560	None:intergenic	45.0%
	CGAGCTTCAACCTACGAAGT+TGG	+	chr3_2:82662376-82662395	Msa0430780:CDS	50.0%
	TGGAAGAAGTTGCTGAGTGG+TGG	+	chr3_2:82662396-82662415	Msa0430780:CDS	50.0%
	GGAAGAAGTTGCTGAGTGGT+GGG	+	chr3_2:82662397-82662416	Msa0430780:CDS	50.0%
!!!	TGGGTTGCGGATGCTACTTT+TGG	+	chr3_2:82662416-82662435	Msa0430780:CDS	50.0%
!!	GGCGAAAGAGCATGGGTTTT+TGG	+	chr3_2:82662470-82662489	Msa0430780:CDS	50.0%
!!	AGTTGCTGAGTGGTGGGTTG+CGG	+	chr3_2:82662403-82662422	Msa0430780:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_2	gene	82662329	82662563	82662329	ID=Msa0430780;Name=Msa0430780
chr3_2	mRNA	82662329	82662563	82662329	ID=Msa0430780-mRNA-1;Parent=Msa0430780;Name=Msa0430780-mRNA-1;_AED=0.48;_eAED=0.48;_QI=0\|0\|0\|1\|0\|0\|2\|0\|76
chr3_2	exon	82662329	82662452	82662329	ID=Msa0430780-mRNA-1:exon:22991;Parent=Msa0430780-mRNA-1
chr3_2	exon	82662457	82662563	82662457	ID=Msa0430780-mRNA-1:exon:22992;Parent=Msa0430780-mRNA-1
chr3_2	CDS	82662329	82662452	82662329	ID=Msa0430780-mRNA-1:cds;Parent=Msa0430780-mRNA-1
chr3_2	CDS	82662457	82662563	82662457	ID=Msa0430780-mRNA-1:cds;Parent=Msa0430780-mRNA-1

Gene Sequence	>Msa0430780 ATGATGAAGGATAAGGGTCCAGTTTGGGATGAGATTGTGAAGGAGAACGAGCTTCAACCTACGAAGTTGGAAGAAGTTGCTGAGTGGTGGGTTGCGGATGCTACTTTTGGAATGGAAGACATTTATAGCATGAACAAGGCGAAAGAGCATGGGTTTTTGGGATTCAGAAATAGCAAGAATTCGCTCGTAAATTGGATAGACAAGACCAGAGCTTACAAGATTGTTCCGTGA
Protein sequence	>Msa0430780 MMKDKGPVWDEIVKENELQPTKLEEVAEWWVADATFGMEDIYSMNKAKEHGFLGFRNSKNSLVNWIDKTRAYKIVP*