AlfalfaGEDB | Msa0618220

M. sativa cultivar zhongmu-4 / Msa0618220

KEGG: -
GO: GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009534 GO:0009536 GO:0009579 GO:0030312 GO:0031976 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048046 GO:0071944
PFAM: PF00657.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0618220	XP_003629878.1	96.296	351	13	0	1	351	1	351	0.0	699

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0618220	sp\|Q9C7N4\|GDL15_ARATH	45.902	366	180	8	1	351	1	363	1.47e-107	322

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0044100	Msa0618220	0.824672	7.111167e-54	-8.615850e-47
Msa0141100	Msa0618220	0.842775	2.104313e-58	-8.615850e-47
Msa0183750	Msa0618220	0.837078	6.426958e-57	-8.615850e-47
Msa0311690	Msa0618220	0.802017	6.848327e-49	-8.615850e-47
Msa0315250	Msa0618220	0.814166	1.769052e-51	-8.615850e-47
Msa0374850	Msa0618220	0.838271	3.174588e-57	-8.615850e-47
Msa0375090	Msa0618220	0.833101	6.469485e-56	-8.615850e-47
Msa0385670	Msa0618220	0.845352	4.285407e-59	-8.615850e-47
Msa0435670	Msa0618220	0.834539	2.827784e-56	-8.615850e-47
Msa0470340	Msa0618220	0.850037	2.199662e-60	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0618220	MtrunA17_Chr8g0380031	96.296	351	13	0	1	351	56	406	0.0	699
Msa0618220	MtrunA17_Chr2g0284951	59.331	359	132	7	1	350	1	354	2.48e-140	402
Msa0618220	MtrunA17_Chr2g0284991	58.056	360	140	7	1	350	1	359	3.49e-138	397
Msa0618220	MtrunA17_Chr2g0284941	55.710	359	129	7	1	350	1	338	1.25e-127	370
Msa0618220	MtrunA17_Chr2g0285001	54.571	361	152	8	1	350	1	360	4.73e-127	369
Msa0618220	MtrunA17_Chr2g0284921	54.270	363	152	9	1	351	1	361	1.10e-125	365
Msa0618220	MtrunA17_Chr3g0085881	52.941	357	156	7	1	350	1	352	2.83e-125	363
Msa0618220	MtrunA17_Chr3g0086641	47.514	362	175	6	1	351	13	370	5.48e-113	333
Msa0618220	MtrunA17_Chr8g0380041	49.415	342	161	5	21	351	23	363	2.46e-112	331
Msa0618220	MtrunA17_Chr2g0285061	47.478	337	166	4	26	351	29	365	2.16e-106	316

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0618220	AT1G29670.1	45.902	366	180	8	1	351	1	363	1.49e-108	322
Msa0618220	AT1G29670.2	45.902	366	180	8	1	351	69	431	2.15e-107	321
Msa0618220	AT1G29660.1	43.137	357	188	5	6	351	12	364	1.94e-105	314
Msa0618220	AT4G18970.1	45.588	340	174	4	23	351	22	361	1.13e-103	309
Msa0618220	AT4G18970.2	45.588	340	174	4	23	351	22	361	1.26e-102	308
Msa0618220	AT5G45670.1	43.539	356	188	5	9	351	7	362	7.42e-102	305
Msa0618220	AT1G33811.1	43.490	361	186	9	7	351	12	370	9.96e-98	294
Msa0618220	AT2G19010.1	41.261	349	188	7	8	350	10	347	1.51e-84	260
Msa0618220	AT2G19010.2	41.261	349	188	7	8	350	40	377	6.36e-84	259
Msa0618220	AT2G19050.1	42.722	316	162	5	26	332	28	333	2.19e-82	254

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Find 95 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTTAATTACAGGCCTTA+TGG	0.291310	4_2:-69099435	Msa0618220:CDS
AAATGAAAAGACATGGAACT+TGG	0.425433	4_2:+69099511	None:intergenic
AAGTTAATTACAGGCCTTAT+GGG	0.326292	4_2:-69099434	Msa0618220:CDS
AATCTTCCCGTAGGACCAGC+AGG	0.519591	4_2:+69099402	None:intergenic
AATGAAAAGACATGGAACTT+GGG	0.513164	4_2:+69099512	None:intergenic
AATTATGCATCCGGTGCAGC+AGG	0.432883	4_2:-69098930	Msa0618220:CDS
AATTTGTTGACCACCTCAGT+TGG	0.533120	4_2:+69096223	None:intergenic
ACAAGACGACAGATATCCAT+TGG	0.523223	4_2:+69096154	None:intergenic
ACTGTTAATGAAGATGAATT+TGG	0.317652	4_2:+69097115	None:intergenic
AGAATTATCAACTTATTTAA+AGG	0.193593	4_2:-69098452	Msa0618220:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGGATCATAAGACTAAGTTA+TGG	-	chr4_2:69095872-69095891	Msa0618220:three_prime_UTR	30.0%
TCATAAGACTAAGTTATGGA+TGG	-	chr4_2:69095876-69095895	Msa0618220:three_prime_UTR	30.0%
AATGAAAAGACATGGAACTT+GGG	+	chr4_2:69095947-69095966	None:intergenic	30.0%
AAATGAAAAGACATGGAACT+TGG	+	chr4_2:69095948-69095967	None:intergenic	30.0%
TCTCCAAAAATGAAAAGACA+TGG	+	chr4_2:69095955-69095974	None:intergenic	30.0%
ATAAGGCCTGTAATTAACTT+TGG	+	chr4_2:69096022-69096041	None:intergenic	30.0%
AAAGTTAATTACAGGCCTTA+TGG	-	chr4_2:69096021-69096040	Msa0618220:three_prime_UTR	30.0%
TGATTATGTCAACTGAAGTA+CGG	+	chr4_2:69096089-69096108	None:intergenic	30.0%
CGCCCAATATAAAAATTTGT+CGG	+	chr4_2:69096144-69096163	None:intergenic	30.0%
TACAAAGACATGTATAAGGT+AGG	+	chr4_2:69096709-69096728	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	69095831	69099647	69095831	ID=Msa0618220;Name=Msa0618220
chr4_2	mRNA	69095831	69099647	69095831	ID=Msa0618220-mRNA-1;Parent=Msa0618220;Name=Msa0618220-mRNA-1;_AED=0.02;_eAED=0.02;_QI=40\|1\|1\|1\|1\|1\|5\|313\|351
chr4_2	exon	69095831	69096346	69095831	ID=Msa0618220-mRNA-1:exon:19934;Parent=Msa0618220-mRNA-1
chr4_2	exon	69097081	69097327	69097081	ID=Msa0618220-mRNA-1:exon:19933;Parent=Msa0618220-mRNA-1
chr4_2	exon	69098453	69098695	69098453	ID=Msa0618220-mRNA-1:exon:19932;Parent=Msa0618220-mRNA-1
chr4_2	exon	69098902	69099026	69098902	ID=Msa0618220-mRNA-1:exon:19931;Parent=Msa0618220-mRNA-1
chr4_2	exon	69099370	69099647	69099370	ID=Msa0618220-mRNA-1:exon:19930;Parent=Msa0618220-mRNA-1
chr4_2	five_prime_UTR	69099608	69099647	69099608	ID=Msa0618220-mRNA-1:five_prime_utr;Parent=Msa0618220-mRNA-1
chr4_2	CDS	69099370	69099607	69099370	ID=Msa0618220-mRNA-1:cds;Parent=Msa0618220-mRNA-1
chr4_2	CDS	69098902	69099026	69098902	ID=Msa0618220-mRNA-1:cds;Parent=Msa0618220-mRNA-1
chr4_2	CDS	69098453	69098695	69098453	ID=Msa0618220-mRNA-1:cds;Parent=Msa0618220-mRNA-1
chr4_2	CDS	69097081	69097327	69097081	ID=Msa0618220-mRNA-1:cds;Parent=Msa0618220-mRNA-1
chr4_2	CDS	69096144	69096346	69096144	ID=Msa0618220-mRNA-1:cds;Parent=Msa0618220-mRNA-1
chr4_2	three_prime_UTR	69095831	69096143	69095831	ID=Msa0618220-mRNA-1:three_prime_utr;Parent=Msa0618220-mRNA-1

Gene Sequence

>Msa0618220

ATGGATCATAAGACTAAGTTATGGATGGTGCTTTTCTTCTTCTCAAGTGCAACATATTGTGTTGTTGGGAAGCCCCAAGTTCCATGTCTTTTCATTTTTGGAGATTCTTTATCGGACAGTGGAAACAACAATAACCTAGCTACCGATGCCAAAGTTAATTACAGGCCTTATGGGATTGATTTTCCTGCTGGTCCTACGGGAAGATTTACCAATGGCCGTACTTCAGTTGACATAATCACTGAACTTTTGGGATTTGACCACTTTATCCCACCTTATGCAAATACCCATGGTGCAGACATAGTTCAAGGTGTTAATTATGCATCCGGTGCAGCAGGGATCCGCAATGAGACTGGAACACACTTGGGTCCAGATATCAGCATGGGATTGCAGTTACAACATCACACAGACATTGTTTCCCAAATTGCTAAGAAACTTGGATATGACAAAGTGCAACAACACCTTAATAAGTGTTTGTATTATGTGAATATAGGCAGCAACGATTACCTTAACAATTACCTCCTGCCACAACATTATCCAACTAAAGGAAAGTACACTACTGATCAGTATGCTGCAGCTCTTGTCCAAGAATTATCAACTTATTTAAAGGGTATACATGGGCTTGGAGCAAGAAAGTTTTCCTTAGTTGGATTAGGCCTTCTAGGTTGCGTTCCACATGAAATTTCTACCCATGGGAAAAATGATTCAAGGTGTATTCAAGAGGAGAATAATGCAGCACTACTTTTCAATGACAAACTTAAAGCCCTCGTTGATCGTTTGAATAAAGAATTAACTGATTCCAAATTCATCTTCATTAACAGTGCTGTCATAAGACATAGTCAGTTAAAGCTACCAGATCTTGTGAAATGTTGCAAAGTGGGGTCAAACGGACAGTGTATCCCTAATACGAAACCGTGCAAAGCAAGAAACCTACATCCATTTTTTGATGCATTTCATCCAACTGAGGTGGTCAACAAATTGTCTGCAAACTTAGCTTATAATGCTCCTGCTCCATCCTTTGCATATCCAATGGATATCTGTCGTCTTGTTAAATTGTAA

Protein sequence

>Msa0618220

MDHKTKLWMVLFFFSSATYCVVGKPQVPCLFIFGDSLSDSGNNNNLATDAKVNYRPYGIDFPAGPTGRFTNGRTSVDIITELLGFDHFIPPYANTHGADIVQGVNYASGAAGIRNETGTHLGPDISMGLQLQHHTDIVSQIAKKLGYDKVQQHLNKCLYYVNIGSNDYLNNYLLPQHYPTKGKYTTDQYAAALVQELSTYLKGIHGLGARKFSLVGLGLLGCVPHEISTHGKNDSRCIQEENNAALLFNDKLKALVDRLNKELTDSKFIFINSAVIRHSQLKLPDLVKCCKVGSNGQCIPNTKPCKARNLHPFFDAFHPTEVVNKLSANLAYNAPAPSFAYPMDICRLVKL*