AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0701970

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0701970 MtrunA17_Chr8g0380031 81.766 351 11 1 1 298 56 406 0.0 568
Msa0701970 MtrunA17_Chr2g0284951 50.139 359 112 8 1 297 1 354 2.20e-104 309
Msa0701970 MtrunA17_Chr2g0284991 48.611 360 121 8 1 297 1 359 7.07e-101 300
Msa0701970 MtrunA17_Chr2g0284921 46.281 363 128 10 1 298 1 361 2.92e-94 283
Msa0701970 MtrunA17_Chr2g0285001 45.983 361 130 9 1 297 1 360 2.84e-92 278
Msa0701970 MtrunA17_Chr3g0085881 45.098 357 131 8 1 297 1 352 5.32e-92 277
Msa0701970 MtrunA17_Chr2g0284941 45.404 359 113 8 1 297 1 338 6.66e-89 269
Msa0701970 MtrunA17_Chr3g0086641 41.274 361 146 6 1 298 13 370 1.80e-86 263
Msa0701970 MtrunA17_Chr8g0380041 41.813 342 134 6 21 298 23 363 1.01e-81 251
Msa0701970 MtrunA17_Chr2g0285061 40.950 337 135 5 26 298 29 365 1.35e-79 245
Msa0701970 MtrunA17_Chr3g0077581 38.315 368 147 14 5 298 9 370 2.17e-65 209
Msa0701970 MtrunA17_Chr3g0077571 36.798 356 156 10 5 298 9 357 7.04e-62 199
Msa0701970 MtrunA17_Chr3g0131321 35.278 360 160 11 7 297 13 368 2.16e-58 191
Msa0701970 MtrunA17_Chr4g0017431 30.702 342 170 9 22 298 24 363 2.12e-44 154
Msa0701970 MtrunA17_Chr7g0275021 30.448 335 166 7 29 298 34 366 1.72e-43 152
Msa0701970 MtrunA17_Chr4g0068441 31.564 358 170 10 7 297 18 367 4.58e-43 151
Msa0701970 MtrunA17_Chr5g0438641 33.229 319 139 7 26 275 29 342 9.37e-42 147
Msa0701970 MtrunA17_Chr5g0438621 32.537 335 157 10 8 276 10 341 1.18e-40 144
Msa0701970 MtrunA17_Chr7g0274991 28.863 343 177 7 21 298 25 365 1.84e-39 142
Msa0701970 MtrunA17_Chr5g0396511 29.607 331 167 6 31 296 48 377 2.56e-39 141
Msa0701970 MtrunA17_Chr7g0275011 27.887 355 189 7 9 298 14 366 1.57e-38 139
Msa0701970 MtrunA17_Chr2g0320671 31.579 342 167 8 1 276 1 341 3.32e-38 138
Msa0701970 MtrunA17_Chr7g0275001 28.134 359 190 7 5 298 3 358 4.32e-38 138
Msa0701970 MtrunA17_Chr5g0434621 32.258 310 142 9 31 275 27 333 6.76e-38 137
Msa0701970 MtrunA17_Chr2g0294311 29.360 344 167 9 28 297 31 372 6.81e-38 137
Msa0701970 MtrunA17_Chr8g0374101 30.435 345 162 8 7 275 12 354 7.37e-38 137
Msa0701970 MtrunA17_Chr6g0457921 29.552 335 168 7 30 298 30 362 1.01e-37 137
Msa0701970 MtrunA17_Chr1g0156921 28.701 331 170 7 7 272 20 349 1.06e-37 137
Msa0701970 MtrunA17_Chr1g0189901 30.097 309 150 6 30 273 32 339 1.22e-37 137
Msa0701970 MtrunA17_Chr3g0120861 30.312 320 157 6 22 276 31 349 2.67e-37 135
Msa0701970 MtrunA17_Chr1g0156901 28.443 334 172 8 4 272 25 356 2.94e-37 135
Msa0701970 MtrunA17_Chr2g0294301 30.973 339 164 10 26 297 32 367 7.26e-37 135
Msa0701970 MtrunA17_Chr3g0105401 31.454 337 161 11 7 276 7 340 7.75e-37 134
Msa0701970 MtrunA17_Chr1g0156911 27.679 336 175 9 10 278 15 349 3.34e-36 132
Msa0701970 MtrunA17_Chr5g0438631 30.769 338 162 12 7 276 7 340 1.05e-35 131
Msa0701970 MtrunA17_Chr1g0156931 28.746 327 166 9 8 268 17 342 1.48e-34 128
Msa0701970 MtrunA17_Chr5g0444401 27.095 358 183 12 9 296 11 360 1.52e-34 128
Msa0701970 MtrunA17_Chr1g0189931 27.463 335 176 7 29 298 32 364 2.18e-34 128
Msa0701970 MtrunA17_Chr1g0189891 30.000 330 167 9 30 298 30 356 2.21e-34 128
Msa0701970 MtrunA17_Chr3g0099161 28.117 377 176 14 1 298 66 426 2.60e-34 129
Msa0701970 MtrunA17_Chr4g0060811 32.063 315 140 11 31 276 29 338 8.83e-33 124
Msa0701970 MtrunA17_Chr5g0405831 28.571 308 153 7 27 268 41 347 1.50e-32 123
Msa0701970 MtrunA17_Chr3g0099151 27.195 353 163 13 26 298 84 422 2.67e-32 123
Msa0701970 MtrunA17_Chr4g0008851 26.359 368 199 10 1 298 1 366 2.70e-32 122
Msa0701970 MtrunA17_Chr3g0142921 29.316 307 153 10 28 270 35 341 4.91e-32 122
Msa0701970 MtrunA17_Chr5g0432101 27.711 332 172 9 24 288 28 358 6.43e-32 121
Msa0701970 MtrunA17_Chr5g0420811 31.046 306 152 11 26 275 30 332 1.39e-31 120
Msa0701970 MtrunA17_Chr1g0182811 27.679 336 174 9 30 298 34 367 1.61e-31 120
Msa0701970 MtrunA17_Chr3g0099231 26.027 365 194 13 1 296 2 359 5.44e-31 119
Msa0701970 MtrunA17_Chr4g0027521 29.721 323 158 8 21 275 19 340 6.66e-31 118
Msa0701970 MtrunA17_Chr3g0142941 30.547 311 149 9 27 272 45 353 9.78e-31 118
Msa0701970 MtrunA17_Chr3g0085231 30.678 339 157 12 23 297 25 349 1.03e-30 118
Msa0701970 MtrunA17_Chr1g0156891 29.042 334 166 10 8 272 19 350 2.43e-30 117
Msa0701970 MtrunA17_Chr3g0092131 31.646 316 138 13 31 275 25 333 3.32e-30 117
Msa0701970 MtrunA17_Chr2g0330781 29.114 316 157 8 27 276 29 343 4.12e-30 116
Msa0701970 MtrunA17_Chr8g0341001 27.907 344 179 9 1 276 2 344 7.30e-30 115
Msa0701970 MtrunA17_Chr8g0370951 27.988 343 173 10 26 297 28 367 3.17e-29 114
Msa0701970 MtrunA17_Chr8g0371481 26.488 336 176 8 8 273 16 350 6.44e-29 113
Msa0701970 MtrunA17_Chr8g0371491 26.513 347 189 10 5 286 12 357 1.10e-28 112
Msa0701970 MtrunA17_Chr7g0229591 36.905 168 97 5 1 162 1 165 2.26e-28 108
Msa0701970 MtrunA17_Chr8g0344931 28.182 330 170 8 30 298 38 361 5.86e-28 110
Msa0701970 MtrunA17_Chr8g0371471 26.282 312 162 8 28 273 40 349 3.46e-27 108
Msa0701970 MtrunA17_Chr6g0455661 47.500 120 60 2 1 118 1 119 3.83e-27 110
Msa0701970 MtrunA17_Chr6g0455661 34.591 159 92 4 149 298 317 472 1.33e-18 85.5
Msa0701970 MtrunA17_Chr5g0405821 27.217 327 170 8 27 286 41 366 4.10e-27 108
Msa0701970 MtrunA17_Chr7g0215961 26.176 340 173 8 23 298 27 352 5.31e-27 108
Msa0701970 MtrunA17_Chr2g0284931 30.876 217 111 6 121 298 4 220 1.11e-26 104
Msa0701970 MtrunA17_Chr4g0027561 43.802 121 64 3 1 117 1 121 1.21e-26 102
Msa0701970 MtrunA17_Chr6g0469511 46.281 121 61 3 19 136 20 139 3.27e-26 100
Msa0701970 MtrunA17_Chr8g0349331 30.742 283 164 9 30 281 36 317 4.01e-26 105
Msa0701970 MtrunA17_Chr1g0157001 27.946 297 152 6 34 268 41 337 8.27e-26 105
Msa0701970 MtrunA17_Chr8g0349321 27.619 315 162 9 30 281 38 349 1.10e-23 99.4
Msa0701970 MtrunA17_Chr8g0370981 25.330 379 169 9 28 296 43 417 1.74e-23 99.4
Msa0701970 MtrunA17_Chr2g0301641 29.290 338 160 12 30 295 44 374 5.75e-23 97.8
Msa0701970 MtrunA17_Chr2g0285051 72.414 58 16 0 26 83 33 90 1.39e-20 85.1
Msa0701970 MtrunA17_Chr8g0349301 28.996 269 130 8 7 219 8 271 2.09e-20 89.4
Msa0701970 MtrunA17_Chr8g0349341 26.032 315 167 8 30 281 38 349 3.69e-20 89.4
Msa0701970 MtrunA17_Chr2g0283191 24.921 317 163 10 26 268 35 350 5.39e-20 89.0
Msa0701970 MtrunA17_Chr8g0344941 27.331 311 157 10 29 272 41 349 1.22e-19 88.2
Msa0701970 MtrunA17_Chr6g0459921 35.000 160 90 5 149 298 206 361 2.97e-19 86.7
Msa0701970 MtrunA17_Chr1g0189921 43.000 100 55 2 29 126 31 130 3.46e-19 82.4
Msa0701970 MtrunA17_Chr2g0285041 57.576 66 27 1 82 146 1 66 6.12e-19 81.6
Msa0701970 MtrunA17_Chr4g0046151 24.290 317 167 10 24 268 31 346 2.21e-18 84.3
Msa0701970 MtrunA17_Chr2g0308201 51.899 79 31 2 6 79 9 85 6.51e-18 77.4
Msa0701970 MtrunA17_Chr8g0370971 43.878 98 48 2 28 118 37 134 3.99e-17 77.4
Msa0701970 MtrunA17_Chr5g0437421 24.601 313 165 8 26 268 29 340 5.39e-17 80.5
Msa0701970 MtrunA17_Chr2g0321991 47.253 91 43 3 26 112 30 119 8.15e-17 75.5
Msa0701970 MtrunA17_Chr4g0018561 45.570 79 41 2 1 77 1 79 1.89e-14 68.2
Msa0701970 MtrunA17_Chr8g0370961 29.310 174 96 6 115 275 64 223 6.93e-14 70.1
Msa0701970 MtrunA17_Chr1g0198351 24.771 327 171 13 8 268 16 333 8.86e-14 70.9
Msa0701970 MtrunA17_Chr8g0352901 24.561 285 146 7 7 226 6 286 1.91e-13 70.1
Msa0701970 MtrunA17_Chr1g0178941 25.490 306 154 13 30 268 41 339 5.10e-13 68.6
Msa0701970 MtrunA17_Chr7g0229581 31.206 141 72 4 151 273 8 141 2.17e-12 63.9
Msa0701970 MtrunA17_Chr4g0027571 29.861 144 90 3 141 273 65 208 2.59e-12 65.1
Msa0701970 MtrunA17_Chr7g0275291 22.500 360 186 12 1 273 1 354 3.84e-12 66.2
Msa0701970 MtrunA17_Chr2g0285031 47.619 63 32 1 236 297 15 77 8.91e-12 60.1
Msa0701970 MtrunA17_Chr3g0104801 32.520 123 74 4 29 143 44 165 2.88e-11 61.2
Msa0701970 MtrunA17_Chr4g0027531 47.619 63 31 2 1 61 1 63 5.60e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0701970 AT1G29670.1 39.891 366 149 9 1 298 1 363 8.75e-81 249
Msa0701970 AT1G29670.2 39.891 366 149 9 1 298 69 431 8.12e-80 248
Msa0701970 AT1G29660.1 36.415 357 159 6 6 298 12 364 3.96e-77 239
Msa0701970 AT4G18970.1 39.412 340 142 5 23 298 22 361 8.07e-77 238
Msa0701970 AT4G18970.2 39.412 340 142 5 23 298 22 361 7.14e-76 238
Msa0701970 AT5G45670.1 37.921 356 155 6 9 298 7 362 1.05e-75 236
Msa0701970 AT1G33811.1 37.673 361 154 11 7 298 12 370 3.65e-69 219
Msa0701970 AT2G19010.1 35.860 343 162 8 8 297 10 347 8.99e-60 194
Msa0701970 AT2G19010.2 35.860 343 162 8 8 297 40 377 4.50e-59 193
Msa0701970 AT1G71691.2 37.015 335 144 8 27 298 53 383 1.28e-57 190
Msa0701970 AT2G19050.1 36.129 310 138 7 26 279 28 333 5.75e-55 182
Msa0701970 AT4G30140.1 36.308 325 148 7 26 297 30 348 6.17e-55 182
Msa0701970 AT5G15720.1 37.026 343 145 12 24 298 25 364 1.20e-54 181
Msa0701970 AT2G19050.2 39.834 241 97 4 26 219 28 267 6.21e-51 169
Msa0701970 AT1G71250.1 33.528 343 161 10 21 298 33 373 6.33e-49 167
Msa0701970 AT2G19060.1 34.756 328 149 9 27 296 28 348 7.38e-49 166
Msa0701970 AT5G08460.1 32.964 361 169 12 8 298 28 385 2.47e-48 166
Msa0701970 AT1G71691.3 36.508 252 104 3 23 221 49 297 2.80e-42 148
Msa0701970 AT4G28780.1 29.248 359 183 9 8 298 8 363 4.16e-42 149
Msa0701970 AT2G04570.1 30.966 352 174 9 1 286 1 349 4.78e-42 148
Msa0701970 AT5G18430.1 30.330 333 162 7 7 273 10 338 2.14e-39 142
Msa0701970 AT5G33370.1 28.212 358 190 7 6 298 7 362 4.35e-39 141
Msa0701970 AT5G15720.2 36.704 267 109 8 24 232 25 289 5.89e-39 139
Msa0701970 AT3G04290.1 29.532 342 170 10 25 298 23 361 7.34e-39 140
Msa0701970 AT2G42990.1 30.793 328 155 7 17 276 16 339 1.38e-37 136
Msa0701970 AT4G10950.2 29.042 334 169 10 25 292 67 398 3.61e-37 137
Msa0701970 AT5G37690.1 33.866 313 135 11 31 276 29 336 6.07e-36 132
Msa0701970 AT4G26790.1 30.696 316 153 7 26 276 26 340 1.70e-35 131
Msa0701970 AT4G26790.2 30.696 316 153 7 26 276 26 340 1.70e-35 131
Msa0701970 AT4G16230.1 37.288 236 87 7 10 189 15 245 3.58e-35 127
Msa0701970 AT4G10950.1 29.042 334 165 11 25 292 67 394 7.07e-35 130
Msa0701970 AT3G14820.1 32.248 307 138 11 27 268 30 331 1.64e-34 128
Msa0701970 AT1G75880.2 29.573 328 162 9 9 268 29 355 5.44e-34 127
Msa0701970 AT1G75880.1 29.268 328 164 8 9 268 29 356 2.26e-33 126
Msa0701970 AT5G03820.1 27.429 350 173 9 3 276 2 346 3.57e-33 125
Msa0701970 AT5G42170.1 30.284 317 153 8 27 276 48 363 5.48e-33 125
Msa0701970 AT5G03820.2 27.429 350 173 9 3 276 30 374 7.70e-33 124
Msa0701970 AT3G50400.1 30.321 343 164 10 9 276 11 353 9.11e-33 124
Msa0701970 AT5G41890.1 32.399 321 141 9 31 275 33 353 9.17e-33 124
Msa0701970 AT5G22810.1 29.193 322 159 9 23 276 28 348 1.20e-32 124
Msa0701970 AT1G20132.1 29.503 322 153 9 28 280 60 376 1.92e-32 124
Msa0701970 AT1G71691.1 30.986 284 129 8 78 298 3 282 2.15e-32 121
Msa0701970 AT3G43550.1 33.708 267 121 8 7 219 6 270 4.22e-32 120
Msa0701970 AT1G58520.3 30.537 298 149 8 27 268 28 323 4.60e-32 122
Msa0701970 AT1G58525.1 30.537 298 149 8 27 268 28 323 4.60e-32 122
Msa0701970 AT1G59406.1 30.422 332 165 11 1 268 1 330 8.75e-32 121
Msa0701970 AT1G59030.1 30.422 332 165 11 1 268 1 330 8.75e-32 121
Msa0701970 AT1G58725.1 30.422 332 165 11 1 268 1 330 8.75e-32 121
Msa0701970 AT2G03980.2 29.936 314 150 10 28 275 42 351 1.64e-31 120
Msa0701970 AT2G03980.3 29.936 314 150 10 28 275 42 351 1.64e-31 120
Msa0701970 AT2G03980.1 29.936 314 150 10 28 275 42 351 1.64e-31 120
Msa0701970 AT2G03980.4 29.936 314 150 10 28 275 42 351 1.64e-31 120
Msa0701970 AT1G74460.1 31.310 313 147 10 31 277 25 335 1.72e-31 120
Msa0701970 AT5G37690.2 31.949 313 133 12 31 276 29 328 3.96e-31 119
Msa0701970 AT1G75890.2 28.739 341 170 11 7 275 19 358 4.47e-31 119
Msa0701970 AT5G15720.3 32.258 279 120 10 88 298 56 333 6.71e-31 118
Msa0701970 AT1G75890.3 28.655 342 171 11 7 276 48 388 7.28e-31 119
Msa0701970 AT3G53100.1 27.378 347 142 11 16 275 16 339 1.04e-30 118
Msa0701970 AT2G23540.1 28.000 350 169 10 8 275 17 365 9.34e-29 114
Msa0701970 AT1G75900.1 27.193 342 181 8 6 280 17 357 1.64e-28 112
Msa0701970 AT1G75890.1 27.401 354 173 12 7 276 19 372 1.79e-28 112
Msa0701970 AT5G33370.2 29.259 270 135 4 6 220 7 275 3.86e-28 111
Msa0701970 AT1G20120.1 27.974 311 158 9 28 273 77 386 2.79e-27 110
Msa0701970 AT1G58430.1 26.480 321 168 8 24 277 30 349 7.84e-27 108
Msa0701970 AT2G24560.1 27.329 322 168 10 22 277 27 348 8.52e-27 108
Msa0701970 AT5G03810.1 26.562 320 168 8 23 276 24 342 1.33e-26 107
Msa0701970 AT3G16370.1 27.302 315 162 8 27 275 28 341 1.93e-26 107
Msa0701970 AT4G16220.1 53.982 113 45 4 9 119 14 121 2.07e-26 104
Msa0701970 AT1G73610.1 27.922 308 149 9 23 268 29 325 2.96e-26 106
Msa0701970 AT5G63170.1 28.750 320 156 10 11 268 10 319 4.68e-26 105
Msa0701970 AT1G20130.2 28.213 319 154 10 27 272 182 498 5.11e-26 107
Msa0701970 AT1G20130.1 28.213 319 154 10 27 272 202 518 5.36e-26 107
Msa0701970 AT2G40250.1 25.077 323 172 8 25 278 32 353 7.08e-26 105
Msa0701970 AT2G30220.1 26.480 321 168 10 24 277 28 347 8.83e-26 105
Msa0701970 AT1G06990.1 26.948 308 158 11 28 269 36 342 1.01e-25 105
Msa0701970 AT1G23500.1 30.351 313 162 8 10 268 16 326 2.45e-25 103
Msa0701970 AT5G55050.1 27.193 342 173 12 27 296 37 374 2.74e-25 104
Msa0701970 AT1G20135.1 27.759 299 142 8 33 268 33 320 2.74e-25 103
Msa0701970 AT2G30310.1 26.603 312 161 10 24 268 29 339 1.23e-24 102
Msa0701970 AT2G31540.1 26.480 321 168 8 24 277 30 349 1.84e-24 101
Msa0701970 AT2G24560.2 29.644 253 122 8 23 219 28 280 1.23e-23 98.6
Msa0701970 AT1G75930.1 28.758 306 152 9 30 272 30 332 1.91e-23 98.6
Msa0701970 AT3G43570.1 41.732 127 70 3 27 150 28 153 9.05e-23 96.3
Msa0701970 AT1G75920.2 27.729 339 173 11 1 275 1 331 5.77e-21 91.7
Msa0701970 AT1G53920.1 26.048 334 177 9 15 281 34 364 1.23e-20 91.3
Msa0701970 AT1G71120.1 25.545 321 166 8 27 281 29 342 3.62e-20 89.7
Msa0701970 AT5G45960.1 26.433 314 161 9 24 268 42 354 3.70e-20 89.7
Msa0701970 AT1G75910.1 28.614 332 159 12 5 268 3 324 7.65e-19 85.9
Msa0701970 AT2G04020.2 29.856 278 147 11 28 271 42 305 8.56e-19 85.5
Msa0701970 AT5G45950.1 25.000 328 180 13 21 286 32 355 1.67e-18 85.1
Msa0701970 AT3G14225.2 24.000 350 183 10 24 298 32 373 6.44e-18 83.6
Msa0701970 AT5G14450.1 24.931 361 178 14 8 281 19 373 1.13e-16 80.1
Msa0701970 AT1G75920.1 26.437 348 169 12 7 275 2 341 1.17e-16 79.7
Msa0701970 AT5G40990.1 25.460 326 169 8 29 283 37 359 1.20e-16 79.7
Msa0701970 AT2G04020.1 39.316 117 66 3 28 140 42 157 2.15e-16 77.8
Msa0701970 AT1G28570.1 34.965 143 77 6 9 136 8 149 2.64e-15 75.9
Msa0701970 AT3G09930.2 27.303 304 148 13 30 268 40 335 5.19e-15 74.7
Msa0701970 AT3G09930.1 27.303 304 148 13 30 268 40 335 5.19e-15 74.7
Msa0701970 AT1G75920.3 27.922 308 181 12 1 275 1 300 1.71e-14 73.2
Msa0701970 AT2G27360.1 35.507 138 74 5 9 132 8 144 2.86e-14 72.8
Msa0701970 AT5G03600.2 24.149 323 177 11 7 268 21 336 8.76e-14 71.2
Msa0701970 AT5G03600.1 23.826 298 165 10 26 268 11 301 3.71e-13 69.3
Msa0701970 AT1G28580.1 36.975 119 63 5 33 141 41 157 3.77e-13 69.7
Msa0701970 AT1G31550.1 37.838 111 54 4 33 132 40 146 4.87e-13 69.3
Msa0701970 AT1G31550.2 37.838 111 54 4 33 132 40 146 5.39e-13 69.3
Msa0701970 AT1G28610.1 33.577 137 70 6 13 132 10 142 7.87e-13 67.4
Msa0701970 AT1G28610.2 23.433 367 182 15 13 288 10 368 1.20e-12 68.2
Msa0701970 AT1G53940.1 25.620 242 122 6 29 215 39 277 2.14e-12 67.0
Msa0701970 AT1G28600.2 32.143 140 74 7 9 132 7 141 7.49e-12 64.3
Msa0701970 AT1G28600.1 31.387 137 79 6 9 132 7 141 1.88e-11 64.7
Msa0701970 AT1G28590.1 36.207 116 58 6 33 136 40 151 2.13e-11 64.3
Msa0701970 AT5G03590.2 34.426 122 66 4 1 113 1 117 6.71e-11 61.2

Find 85 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAAGTTTCTTAGCAATTT+TGG 0.108490 4_4:-73421182 None:intergenic
TAAAATTGATATTGTAAATT+TGG 0.109633 4_4:+73423813 Msa0701970:three_prime_UTR
ATATTGTAAATTTGGAAATA+TGG 0.180570 4_4:+73423821 Msa0701970:three_prime_UTR
GCTTTGCACGGTTTCGTATT+AGG 0.181534 4_4:-73423565 None:intergenic
TACTATTGCAGCTGAACTTT+TGG 0.243715 4_4:+73420818 Msa0701970:intron
TTAGTTGGATTAGGCCTATT+AGG 0.251903 4_4:+73422569 Msa0701970:CDS
CTTTGCACGGTTTCGTATTA+GGG 0.271934 4_4:-73423564 None:intergenic
AAAGTTAATTACAGGCCTTA+TGG 0.291310 4_4:+73420399 Msa0701970:CDS
TGCAACCCCATGCTGATATC+TGG 0.302214 4_4:-73421133 None:intergenic
ACTGTTAATGAAGATGAATT+TGG 0.317652 4_4:-73422726 None:intergenic
GAAATATGGAAAGTAGAATA+AGG 0.319984 4_4:+73423835 Msa0701970:three_prime_UTR
CATATGAGTAATTAAGCTTA+TGG 0.323376 4_4:+73423945 Msa0701970:three_prime_UTR
AAGTTAATTACAGGCCTTAT+GGG 0.326292 4_4:+73420400 Msa0701970:CDS
TCTTAGTAGATAAGCTAAAT+AGG 0.356753 4_4:+73423894 Msa0701970:three_prime_UTR
TATATATAAGCAGAACTATA+TGG 0.363670 4_4:-73424022 None:intergenic
CCGATGCCAAAGTTAATTAC+AGG 0.368345 4_4:+73420391 Msa0701970:CDS
TTGCATAAGGTGGGATAAAA+TGG 0.379618 4_4:-73420848 None:intergenic
TCCAAAATTGCTAAGAAACT+TGG 0.386860 4_4:+73421181 Msa0701970:CDS
ATCTACTAAGACATTAAGTA+TGG 0.393756 4_4:-73423882 None:intergenic
CATGTGGAACGCAACCTAAT+AGG 0.400237 4_4:-73422583 None:intergenic
AAATGAAAAGACATGGAACT+TGG 0.425433 4_4:-73420323 None:intergenic
GCAGGGATCCGCAATGAGAC+TGG 0.431672 4_4:+73420921 Msa0701970:CDS
AATTATGCATCCGGTGCAGC+AGG 0.432883 4_4:+73420903 Msa0701970:CDS
ATAAGGCCTGTAATTAACTT+TGG 0.440046 4_4:-73420397 None:intergenic
GAGCAGGCTATACATGGGCT+TGG 0.450690 4_4:+73422530 Msa0701970:intron
CAAGGTGTTAATTATGCATC+CGG 0.468458 4_4:+73420894 Msa0701970:CDS
ATTGCGGATCCCTGCTGCAC+CGG 0.476096 4_4:-73420913 None:intergenic
TTGAACTATGTCTGCACCAT+GGG 0.480194 4_4:-73420874 None:intergenic
TGTTGCAAAGTGGGGTCAAA+CGG 0.480806 4_4:+73423532 Msa0701970:CDS
TTGTTAAATTGTAAACAAGA+TGG 0.480914 4_4:+73423710 Msa0701970:exon
GTGCATGAGCAGGCTATACA+TGG 0.486706 4_4:+73422524 Msa0701970:intron
TAACTTTGGCATCGGTAGCT+AGG 0.487931 4_4:-73420383 None:intergenic
AGTGCAACATATTGTGTTGT+TGG 0.496193 4_4:+73420294 Msa0701970:CDS
GCACCATGGGTATTTGCATA+AGG 0.496690 4_4:-73420861 None:intergenic
AATGAAAAGACATGGAACTT+GGG 0.513164 4_4:-73420322 None:intergenic
TATCTACTCATGTAAGAAGA+TGG 0.513460 4_4:+73423751 Msa0701970:three_prime_UTR
TGTAGGTTTCTTGCTTTGCA+CGG 0.515290 4_4:-73423577 None:intergenic
CCATGGGTATTTGCATAAGG+TGG 0.517638 4_4:-73420858 None:intergenic
AATCTTCCCGTAGGACCAGC+AGG 0.519591 4_4:-73420432 None:intergenic
GTGCAACATATTGTGTTGTT+GGG 0.521073 4_4:+73420295 Msa0701970:CDS
ACAAGACGACAGATATCCAT+TGG 0.523223 4_4:-73423691 None:intergenic
CCACATGAAATTTCTACCCA+TGG 0.524099 4_4:+73422599 Msa0701970:CDS
TGGATCATAAGACTAAGTTA+TGG 0.524487 4_4:+73420250 Msa0701970:CDS
CAATGAGACTGGAACACACT+TGG 0.529479 4_4:+73420932 Msa0701970:CDS
GCAGGAAAATCAATCCCATA+AGG 0.529895 4_4:-73420414 None:intergenic
AATTTGTTGACCACCTCAGT+TGG 0.533120 4_4:-73423622 None:intergenic
CATGGTGCAGACATAGTTCA+AGG 0.533410 4_4:+73420876 Msa0701970:CDS
CATAGTCAGTTAAAGCTACC+AGG 0.536879 4_4:+73422761 Msa0701970:CDS
TCCATTGGATATGCAAAGGA+TGG 0.540736 4_4:-73423676 None:intergenic
CACATGAAATTTCTACCCAT+GGG 0.551621 4_4:+73422600 Msa0701970:CDS
GGATATGCAAAGGATGGAGC+AGG 0.552962 4_4:-73423670 None:intergenic
CCTGTAATTAACTTTGGCAT+CGG 0.559604 4_4:-73420391 None:intergenic
CAGGGTCCAGATATCAGCAT+GGG 0.559625 4_4:+73421127 Msa0701970:intron
ATGAAAAGACATGGAACTTG+GGG 0.561482 4_4:-73420321 None:intergenic
TATAAGCAGAACTATATGGA+GGG 0.563350 4_4:-73424018 None:intergenic
TTCCCTTCAAAACTATGTAG+TGG 0.565863 4_4:-73423980 None:intergenic
GTCAACTGAAGTACGGCCAT+TGG 0.569497 4_4:-73420457 None:intergenic
CTTGTGAAATGTTGCAAAGT+GGG 0.573274 4_4:+73423523 Msa0701970:CDS
TGATTATGTCAACTGAAGTA+CGG 0.593606 4_4:-73420464 None:intergenic
CTTGAACTATGTCTGCACCA+TGG 0.598177 4_4:-73420875 None:intergenic
CCATGGGTAGAAATTTCATG+TGG 0.599051 4_4:-73422599 None:intergenic
CATGGGTATTTGCATAAGGT+GGG 0.600399 4_4:-73420857 None:intergenic
GATATCCATTGGATATGCAA+AGG 0.603960 4_4:-73423680 None:intergenic
CCACCTTATGCAAATACCCA+TGG 0.606787 4_4:+73420858 Msa0701970:CDS
TGCATTTCATCCAACTGAGG+TGG 0.612589 4_4:+73423612 Msa0701970:CDS
TCCATCCTTTGCATATCCAA+TGG 0.616422 4_4:+73423675 Msa0701970:CDS
TTTATTCAAACGATCAACGA+GGG 0.616697 4_4:-73422690 None:intergenic
TCATAAGACTAAGTTATGGA+TGG 0.622468 4_4:+73420254 Msa0701970:CDS
TGATTCAAGGTGTATTCAAG+AGG 0.623109 4_4:+73422628 Msa0701970:CDS
ATCTAGTATATGATTATCAG+AGG 0.625891 4_4:-73423777 None:intergenic
ATAAGCAGAACTATATGGAG+GGG 0.629730 4_4:-73424017 None:intergenic
GGAGATTCTTTATCGGACAG+TGG 0.630401 4_4:+73420348 Msa0701970:CDS
TGATGCATTTCATCCAACTG+AGG 0.643283 4_4:+73423609 Msa0701970:CDS
CCAGGGTCCAGATATCAGCA+TGG 0.646651 4_4:+73421126 Msa0701970:intron
ATATAAGCAGAACTATATGG+AGG 0.647527 4_4:-73424019 None:intergenic
CTTTATTCAAACGATCAACG+AGG 0.650020 4_4:-73422691 None:intergenic
TTGTGAAATGTTGCAAAGTG+GGG 0.670003 4_4:+73423524 Msa0701970:CDS
ATTATGCATCCGGTGCAGCA+GGG 0.675210 4_4:+73420904 Msa0701970:CDS
TCTTGTGAAATGTTGCAAAG+TGG 0.678578 4_4:+73423522 Msa0701970:CDS
TAAGTATGGAGATTACACAT+TGG 0.683807 4_4:-73423868 None:intergenic
CCTACGGGAAGATTTACCAA+TGG 0.686839 4_4:+73420441 Msa0701970:CDS
CCATTGGTAAATCTTCCCGT+AGG 0.687811 4_4:-73420441 None:intergenic
TGCATGAGCAGGCTATACAT+GGG 0.691621 4_4:+73422525 Msa0701970:intron
AGGGTCCAGATATCAGCATG+GGG 0.720526 4_4:+73421128 Msa0701970:intron
AGTGTGTTCCAGTCTCATTG+CGG 0.749177 4_4:-73420929 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATTGATATTGTAAATT+TGG + chr4_4:73423813-73423832 Msa0701970:three_prime_UTR 10.0%
!!! TTAAATAAGTTGATAATTCT+TGG - chr4_4:73421451-73421470 None:intergenic 15.0%
!! AGAATTATCAACTTATTTAA+AGG + chr4_4:73421451-73421470 Msa0701970:intron 15.0%
!!! TTTTTAACACACTTTTTTAT+TGG - chr4_4:73421667-73421686 None:intergenic 15.0%
!!! CGTAATTCTTAAATTTTTAT+TGG + chr4_4:73421700-73421719 Msa0701970:intron 15.0%
!! AAAAAACACTTCAAATTATT+TGG - chr4_4:73423297-73423316 None:intergenic 15.0%
!! ATATTGTAAATTTGGAAATA+TGG + chr4_4:73423821-73423840 Msa0701970:three_prime_UTR 15.0%
!!! GACATTTTTTCTCTTTAATA+TGG + chr4_4:73420230-73420249 Msa0701970:exon 20.0%
!! GAAAATATGTTTATAAGTTG+CGG + chr4_4:73420553-73420572 Msa0701970:intron 20.0%
!! TGTAAGATTTGAATTAAGTT+CGG + chr4_4:73420596-73420615 Msa0701970:intron 20.0%
!!! TTTCCTTGTTATCAAAATTT+AGG - chr4_4:73420707-73420726 None:intergenic 20.0%
!!! CATTCTTTAATCAACTTTTT+TGG + chr4_4:73420770-73420789 Msa0701970:intron 20.0%
!! CATAATACAAACACTTATTA+AGG - chr4_4:73421228-73421247 None:intergenic 20.0%
!! TGTTTGTATTATGTGAATAT+AGG + chr4_4:73421235-73421254 Msa0701970:intron 20.0%
!! CTAAATAGAAAGGTAAAAAA+TGG - chr4_4:73421744-73421763 None:intergenic 20.0%
!! AGAAAATCAACTAAATAGAA+AGG - chr4_4:73421754-73421773 None:intergenic 20.0%
!!! AGTTGATTTTCTTTTTGTTT+AGG + chr4_4:73421762-73421781 Msa0701970:intron 20.0%
!!! TTTAGTTTTTTTTTTGGGAT+TGG + chr4_4:73421805-73421824 Msa0701970:intron 20.0%
!!! TATGGACTATTATGTTATAT+TGG + chr4_4:73421828-73421847 Msa0701970:intron 20.0%
!!! ATGGACTATTATGTTATATT+GGG + chr4_4:73421829-73421848 Msa0701970:intron 20.0%
!! CTGATACAATATTGTATATT+AGG + chr4_4:73421854-73421873 Msa0701970:intron 20.0%
!!! GAGTAGATTTTAAGAATAAT+CGG + chr4_4:73422089-73422108 Msa0701970:intron 20.0%
!!! AGTAGATTTTAAGAATAATC+GGG + chr4_4:73422090-73422109 Msa0701970:intron 20.0%
!! ATGAAATCCAAAAAAATGTA+AGG + chr4_4:73422276-73422295 Msa0701970:intron 20.0%
!! TAAAAAATGTTTCAAATACC+TGG - chr4_4:73422782-73422801 None:intergenic 20.0%
!!! ATTGTAAGTTCTAATATTCA+AGG + chr4_4:73423077-73423096 Msa0701970:intron 20.0%
!!! CAATGTTTTTATCTACTTTA+GGG - chr4_4:73423448-73423467 None:intergenic 20.0%
!!! TCAATGTTTTTATCTACTTT+AGG - chr4_4:73423449-73423468 None:intergenic 20.0%
!! TGATTGTATAACATTAACTA+TGG + chr4_4:73423466-73423485 Msa0701970:intron 20.0%
!! TTGTTAAATTGTAAACAAGA+TGG + chr4_4:73423710-73423729 Msa0701970:exon 20.0%
!!! CATTTTTGGAGATTCTTTAT+CGG + chr4_4:73420341-73420360 Msa0701970:CDS 25.0%
!! ATGCCGACAAATTTTTATAT+TGG + chr4_4:73420516-73420535 Msa0701970:intron 25.0%
! CGTCCAATATAAAAATTTGT+CGG - chr4_4:73420522-73420541 None:intergenic 25.0%
! TAAGATTAATTAAGCACCTT+TGG + chr4_4:73420628-73420647 Msa0701970:intron 25.0%
! GCAATCTTAGATTCAAATTT+AGG + chr4_4:73420650-73420669 Msa0701970:intron 25.0%
!! TTAGATTCAAATTTAGGACA+TGG + chr4_4:73420656-73420675 Msa0701970:intron 25.0%
!!! TCTCCTAAATTTTGATAACA+AGG + chr4_4:73420701-73420720 Msa0701970:intron 25.0%
! ATCTTACAAAGACATGTATA+AGG - chr4_4:73421067-73421086 None:intergenic 25.0%
! CACAAATTTATTAGTCACTT+TGG + chr4_4:73421092-73421111 Msa0701970:intron 25.0%
! TTTCAAAAGTTAGAACCATT+TGG + chr4_4:73421508-73421527 Msa0701970:intron 25.0%
! CTCAAAAACTAATTTAACTG+GGG - chr4_4:73421540-73421559 None:intergenic 25.0%
! CCTCAAAAACTAATTTAACT+GGG - chr4_4:73421541-73421560 None:intergenic 25.0%
!!! CCAGTTAAATTAGTTTTTGA+GGG + chr4_4:73421539-73421558 Msa0701970:intron 25.0%
! GAATCAATTTGAATTTCAAC+TGG - chr4_4:73421595-73421614 None:intergenic 25.0%
! TAGATCCCAAATAAATTTAG+CGG + chr4_4:73421626-73421645 Msa0701970:intron 25.0%
! AAATCCCGCTAAATTTATTT+GGG - chr4_4:73421634-73421653 None:intergenic 25.0%
! TAAATCCCGCTAAATTTATT+TGG - chr4_4:73421635-73421654 None:intergenic 25.0%
!!! TTTTTTTTTTGGGATTGGTA+TGG + chr4_4:73421810-73421829 Msa0701970:intron 25.0%
!! AAGCTTCCAAAGTAATTTTA+GGG - chr4_4:73421896-73421915 None:intergenic 25.0%
!! TAAAATTACTTTGGAAGCTT+GGG + chr4_4:73421896-73421915 Msa0701970:intron 25.0%
!!! GTAGATTTTAAGAATAATCG+GGG + chr4_4:73422091-73422110 Msa0701970:intron 25.0%
!!! AAGCTCCCTTTTTTTTTTTT+GGG + chr4_4:73422120-73422139 Msa0701970:intron 25.0%
! TATATGCCATTAGATCATAT+TGG + chr4_4:73422151-73422170 Msa0701970:intron 25.0%
! TAATCCGAACTAAATGTAAA+GGG - chr4_4:73422257-73422276 None:intergenic 25.0%
! ATAATCCGAACTAAATGTAA+AGG - chr4_4:73422258-73422277 None:intergenic 25.0%
!!! TTCAACACCTTACATTTTTT+TGG - chr4_4:73422286-73422305 None:intergenic 25.0%
!!! AAAAAAATGTAAGGTGTTGA+AGG + chr4_4:73422285-73422304 Msa0701970:intron 25.0%
!!! AAAAAATGTAAGGTGTTGAA+GGG + chr4_4:73422286-73422305 Msa0701970:intron 25.0%
! TACATGTGATAATTCATTCA+TGG - chr4_4:73422407-73422426 None:intergenic 25.0%
! ATGAATGAATTATCACATGT+AGG + chr4_4:73422406-73422425 Msa0701970:intron 25.0%
!! AAGTTTTCTTTAGTTGGATT+AGG + chr4_4:73422560-73422579 Msa0701970:CDS 25.0%
! ACTGTTAATGAAGATGAATT+TGG - chr4_4:73422729-73422748 None:intergenic 25.0%
!!! TGATTCGGTATATTTGTTAT+CGG - chr4_4:73422807-73422826 None:intergenic 25.0%
! ATTGAATGTTCATGTTCTTA+AGG - chr4_4:73422879-73422898 None:intergenic 25.0%
! CATGAACATTCAATAGTTTA+TGG + chr4_4:73422885-73422904 Msa0701970:intron 25.0%
!! TAGTTTTTCTTCCAAAAAAG+TGG + chr4_4:73422919-73422938 Msa0701970:intron 25.0%
!!! ACTTATAAAAGCCACTTTTT+TGG - chr4_4:73422933-73422952 None:intergenic 25.0%
!! TATAAAAAGTTGACTTTCTG+CGG - chr4_4:73423014-73423033 None:intergenic 25.0%
! CACTTCAAATTATTTGGTTA+CGG - chr4_4:73423291-73423310 None:intergenic 25.0%
! ATCTAGTATATGATTATCAG+AGG - chr4_4:73423780-73423799 None:intergenic 25.0%
! GAAATATGGAAAGTAGAATA+AGG + chr4_4:73423835-73423854 Msa0701970:three_prime_UTR 25.0%
! ATCTACTAAGACATTAAGTA+TGG - chr4_4:73423885-73423904 None:intergenic 25.0%
! TCTTAGTAGATAAGCTAAAT+AGG + chr4_4:73423894-73423913 Msa0701970:three_prime_UTR 25.0%
! CATATGAGTAATTAAGCTTA+TGG + chr4_4:73423945-73423964 Msa0701970:three_prime_UTR 25.0%
TGGATCATAAGACTAAGTTA+TGG + chr4_4:73420250-73420269 Msa0701970:CDS 30.0%
TCATAAGACTAAGTTATGGA+TGG + chr4_4:73420254-73420273 Msa0701970:CDS 30.0%
AATGAAAAGACATGGAACTT+GGG - chr4_4:73420325-73420344 None:intergenic 30.0%
AAATGAAAAGACATGGAACT+TGG - chr4_4:73420326-73420345 None:intergenic 30.0%
! GTTCCATGTCTTTTCATTTT+TGG + chr4_4:73420327-73420346 Msa0701970:CDS 30.0%
TCTCCAAAAATGAAAAGACA+TGG - chr4_4:73420333-73420352 None:intergenic 30.0%
ATAAGGCCTGTAATTAACTT+TGG - chr4_4:73420400-73420419 None:intergenic 30.0%
AAAGTTAATTACAGGCCTTA+TGG + chr4_4:73420399-73420418 Msa0701970:CDS 30.0%
! AAGTTAATTACAGGCCTTAT+GGG + chr4_4:73420400-73420419 Msa0701970:CDS 30.0%
TGATTATGTCAACTGAAGTA+CGG - chr4_4:73420467-73420486 None:intergenic 30.0%
! TTTGAATCTAAGATTGCCAA+AGG - chr4_4:73420647-73420666 None:intergenic 30.0%
TCCAAAATTGCTAAGAAACT+TGG + chr4_4:73421181-73421200 Msa0701970:CDS 30.0%
! TCCAAGTTTCTTAGCAATTT+TGG - chr4_4:73421185-73421204 None:intergenic 30.0%
AAAAACTAATTTAACTGGGG+CGG - chr4_4:73421537-73421556 None:intergenic 30.0%
!!! CCCAGTTAAATTAGTTTTTG+AGG + chr4_4:73421538-73421557 Msa0701970:intron 30.0%
CCCTCAAAAACTAATTTAAC+TGG - chr4_4:73421542-73421561 None:intergenic 30.0%
AGATCCCAAATAAATTTAGC+GGG + chr4_4:73421627-73421646 Msa0701970:intron 30.0%
! AGCTTCCAAAGTAATTTTAG+GGG - chr4_4:73421895-73421914 None:intergenic 30.0%
! CAAGCTTCCAAAGTAATTTT+AGG - chr4_4:73421897-73421916 None:intergenic 30.0%
! CTAAAATTACTTTGGAAGCT+TGG + chr4_4:73421895-73421914 Msa0701970:intron 30.0%
TTTCCCTATTCGAAATTACA+GGG - chr4_4:73422026-73422045 None:intergenic 30.0%
! TTTTCCCTATTCGAAATTAC+AGG - chr4_4:73422027-73422046 None:intergenic 30.0%
ACATCCAGATGAATAAATCT+GGG + chr4_4:73422063-73422082 Msa0701970:intron 30.0%
!!! GAAGCTCCCTTTTTTTTTTT+TGG + chr4_4:73422119-73422138 Msa0701970:intron 30.0%
!!! AGCTCCCTTTTTTTTTTTTG+GGG + chr4_4:73422121-73422140 Msa0701970:intron 30.0%
ATCCCCCCAAAAAAAAAAAA+GGG - chr4_4:73422128-73422147 None:intergenic 30.0%
AATCCCCCCAAAAAAAAAAA+AGG - chr4_4:73422129-73422148 None:intergenic 30.0%
!!! TTTTTTTTTTTTGGGGGGAT+TGG + chr4_4:73422128-73422147 Msa0701970:intron 30.0%
TAGAGACCAATATGATCTAA+TGG - chr4_4:73422160-73422179 None:intergenic 30.0%
AGTGAGTGTATACTATAAAG+TGG - chr4_4:73422380-73422399 None:intergenic 30.0%
! GCAAGAAAGTTTTCTTTAGT+TGG + chr4_4:73422554-73422573 Msa0701970:CDS 30.0%
TTTATTCAAACGATCAACGA+GGG - chr4_4:73422693-73422712 None:intergenic 30.0%
AATATACCGAATCATTTGAC+AGG + chr4_4:73422813-73422832 Msa0701970:intron 30.0%
ATATACCGAATCATTTGACA+GGG + chr4_4:73422814-73422833 Msa0701970:intron 30.0%
!! TAAGGCATTCTTTTGGTATT+AGG - chr4_4:73422861-73422880 None:intergenic 30.0%
!! ATGTTCTTAAGGCATTCTTT+TGG - chr4_4:73422868-73422887 None:intergenic 30.0%
AAATGAGAATCTGAAAAGAG+CGG + chr4_4:73422983-73423002 Msa0701970:intron 30.0%
ATGCATCAAAAAATGGATGT+AGG - chr4_4:73423597-73423616 None:intergenic 30.0%
GGATGAAATGCATCAAAAAA+TGG - chr4_4:73423604-73423623 None:intergenic 30.0%
TATCTACTCATGTAAGAAGA+TGG + chr4_4:73423751-73423770 Msa0701970:three_prime_UTR 30.0%
TAAGTATGGAGATTACACAT+TGG - chr4_4:73423871-73423890 None:intergenic 30.0%
AGTGCAACATATTGTGTTGT+TGG + chr4_4:73420294-73420313 Msa0701970:CDS 35.0%
! GTGCAACATATTGTGTTGTT+GGG + chr4_4:73420295-73420314 Msa0701970:CDS 35.0%
ATGAAAAGACATGGAACTTG+GGG - chr4_4:73420324-73420343 None:intergenic 35.0%
CCTGTAATTAACTTTGGCAT+CGG - chr4_4:73420394-73420413 None:intergenic 35.0%
AGCTGCAATAGTAGAATTCT+TGG - chr4_4:73420811-73420830 None:intergenic 35.0%
! TACTATTGCAGCTGAACTTT+TGG + chr4_4:73420818-73420837 Msa0701970:intron 35.0%
! ACTATTGCAGCTGAACTTTT+GGG + chr4_4:73420819-73420838 Msa0701970:intron 35.0%
TTGCATAAGGTGGGATAAAA+TGG - chr4_4:73420851-73420870 None:intergenic 35.0%
! CAAGGTGTTAATTATGCATC+CGG + chr4_4:73420894-73420913 Msa0701970:CDS 35.0%
CCACAACATTATCCAACTAA+GGG + chr4_4:73421389-73421408 Msa0701970:intron 35.0%
CAAACCCCCTAAAATTACTT+TGG + chr4_4:73421887-73421906 Msa0701970:intron 35.0%
! GCTTCCAAAGTAATTTTAGG+GGG - chr4_4:73421894-73421913 None:intergenic 35.0%
TTCCCTATTCGAAATTACAG+GGG - chr4_4:73422025-73422044 None:intergenic 35.0%
CACATCCAGATGAATAAATC+TGG + chr4_4:73422062-73422081 Msa0701970:intron 35.0%
TTTAAGAATAATCGGGGTGT+AGG + chr4_4:73422097-73422116 Msa0701970:intron 35.0%
!!! GCTCCCTTTTTTTTTTTTGG+GGG + chr4_4:73422122-73422141 Msa0701970:intron 35.0%
!!! CTCCCTTTTTTTTTTTTGGG+GGG + chr4_4:73422123-73422142 Msa0701970:intron 35.0%
TAGGAGTTTACATCACTTGT+AGG + chr4_4:73422425-73422444 Msa0701970:intron 35.0%
! TTAGTTGGATTAGGCCTATT+AGG + chr4_4:73422569-73422588 Msa0701970:CDS 35.0%
CACATGAAATTTCTACCCAT+GGG + chr4_4:73422600-73422619 Msa0701970:CDS 35.0%
!! CCTTGAATCATTTTTCCCAT+GGG - chr4_4:73422618-73422637 None:intergenic 35.0%
!! ACCTTGAATCATTTTTCCCA+TGG - chr4_4:73422619-73422638 None:intergenic 35.0%
TGATTCAAGGTGTATTCAAG+AGG + chr4_4:73422628-73422647 Msa0701970:CDS 35.0%
CTTTATTCAAACGATCAACG+AGG - chr4_4:73422694-73422713 None:intergenic 35.0%
TTCAACCCTGTCAAATGATT+CGG - chr4_4:73422822-73422841 None:intergenic 35.0%
AGGTGTTCCAAATTTGAACT+TGG + chr4_4:73423097-73423116 Msa0701970:intron 35.0%
GGTGTTCCAAATTTGAACTT+GGG + chr4_4:73423098-73423117 Msa0701970:intron 35.0%
GAAACTCAAACACACACAAA+TGG - chr4_4:73423128-73423147 None:intergenic 35.0%
TCTTGTGAAATGTTGCAAAG+TGG + chr4_4:73423522-73423541 Msa0701970:CDS 35.0%
CTTGTGAAATGTTGCAAAGT+GGG + chr4_4:73423523-73423542 Msa0701970:CDS 35.0%
TTGTGAAATGTTGCAAAGTG+GGG + chr4_4:73423524-73423543 Msa0701970:CDS 35.0%
GATATCCATTGGATATGCAA+AGG - chr4_4:73423683-73423702 None:intergenic 35.0%
!!! AAGCCACTACATAGTTTTGA+AGG + chr4_4:73423977-73423996 Msa0701970:three_prime_UTR 35.0%
!!! AGCCACTACATAGTTTTGAA+GGG + chr4_4:73423978-73423997 Msa0701970:three_prime_UTR 35.0%
TTCCCTTCAAAACTATGTAG+TGG - chr4_4:73423983-73424002 None:intergenic 35.0%
CCGATGCCAAAGTTAATTAC+AGG + chr4_4:73420391-73420410 Msa0701970:CDS 40.0%
GCAGGAAAATCAATCCCATA+AGG - chr4_4:73420417-73420436 None:intergenic 40.0%
!! TATGGGATTGATTTTCCTGC+TGG + chr4_4:73420417-73420436 Msa0701970:CDS 40.0%
CATGGGTATTTGCATAAGGT+GGG - chr4_4:73420860-73420879 None:intergenic 40.0%
TTGAACTATGTCTGCACCAT+GGG - chr4_4:73420877-73420896 None:intergenic 40.0%
!! ACTTTGGCAATGTTGATTCC+AGG + chr4_4:73421108-73421127 Msa0701970:intron 40.0%
!! CTTTGGCAATGTTGATTCCA+GGG + chr4_4:73421109-73421128 Msa0701970:intron 40.0%
TAGTTGGATAATGTTGTGGC+AGG - chr4_4:73421388-73421407 None:intergenic 40.0%
GCCACAACATTATCCAACTA+AGG + chr4_4:73421388-73421407 Msa0701970:intron 40.0%
CCCTTAGTTGGATAATGTTG+TGG - chr4_4:73421392-73421411 None:intergenic 40.0%
GTAGTGTACTTTCCCTTAGT+TGG - chr4_4:73421404-73421423 None:intergenic 40.0%
TTGGGTAGTTGTTGAGTTAG+AGG + chr4_4:73421914-73421933 Msa0701970:intron 40.0%
ACACCCCTGTAATTTCGAAT+AGG + chr4_4:73422019-73422038 Msa0701970:intron 40.0%
CACCCCTGTAATTTCGAATA+GGG + chr4_4:73422020-73422039 Msa0701970:intron 40.0%
TCCAGATGAATAAATCTGGG+TGG + chr4_4:73422066-73422085 Msa0701970:intron 40.0%
CCAGATGAATAAATCTGGGT+GGG + chr4_4:73422067-73422086 Msa0701970:intron 40.0%
! ATTGGTCTCTAATACCATGC+TGG + chr4_4:73422169-73422188 Msa0701970:intron 40.0%
TCTACAGTAAAAGTCCAGCA+TGG - chr4_4:73422186-73422205 None:intergenic 40.0%
TTACTGTAGAACCCAGTTAG+TGG + chr4_4:73422196-73422215 Msa0701970:intron 40.0%
CGCACCCTTTACATTTAGTT+CGG + chr4_4:73422250-73422269 Msa0701970:intron 40.0%
! AGAGTGCTTGAGAAACTAGT+AGG + chr4_4:73422335-73422354 Msa0701970:intron 40.0%
CCATGGGTAGAAATTTCATG+TGG - chr4_4:73422602-73422621 None:intergenic 40.0%
CCACATGAAATTTCTACCCA+TGG + chr4_4:73422599-73422618 Msa0701970:CDS 40.0%
CCCATGGGAAAAATGATTCA+AGG + chr4_4:73422615-73422634 Msa0701970:CDS 40.0%
CATAGTCAGTTAAAGCTACC+AGG + chr4_4:73422761-73422780 Msa0701970:CDS 40.0%
GGAGATCCCAAGTTCAAATT+TGG - chr4_4:73423107-73423126 None:intergenic 40.0%
! GTGTGTGTTTGAGTTTCACT+TGG + chr4_4:73423131-73423150 Msa0701970:intron 40.0%
CTTTGCACGGTTTCGTATTA+GGG - chr4_4:73423567-73423586 None:intergenic 40.0%
TGTAGGTTTCTTGCTTTGCA+CGG - chr4_4:73423580-73423599 None:intergenic 40.0%
TGATGCATTTCATCCAACTG+AGG + chr4_4:73423609-73423628 Msa0701970:CDS 40.0%
!! AATTTGTTGACCACCTCAGT+TGG - chr4_4:73423625-73423644 None:intergenic 40.0%
TCCATCCTTTGCATATCCAA+TGG + chr4_4:73423675-73423694 Msa0701970:CDS 40.0%
TCCATTGGATATGCAAAGGA+TGG - chr4_4:73423679-73423698 None:intergenic 40.0%
ACAAGACGACAGATATCCAT+TGG - chr4_4:73423694-73423713 None:intergenic 40.0%
GGAGATTCTTTATCGGACAG+TGG + chr4_4:73420348-73420367 Msa0701970:CDS 45.0%
!! TAACTTTGGCATCGGTAGCT+AGG - chr4_4:73420386-73420405 None:intergenic 45.0%
! TGATTTTCCTGCTGGTCCTA+CGG + chr4_4:73420425-73420444 Msa0701970:CDS 45.0%
CCATTGGTAAATCTTCCCGT+AGG - chr4_4:73420444-73420463 None:intergenic 45.0%
CCTACGGGAAGATTTACCAA+TGG + chr4_4:73420441-73420460 Msa0701970:CDS 45.0%
CCATGGGTATTTGCATAAGG+TGG - chr4_4:73420861-73420880 None:intergenic 45.0%
CCACCTTATGCAAATACCCA+TGG + chr4_4:73420858-73420877 Msa0701970:CDS 45.0%
GCACCATGGGTATTTGCATA+AGG - chr4_4:73420864-73420883 None:intergenic 45.0%
CTTGAACTATGTCTGCACCA+TGG - chr4_4:73420878-73420897 None:intergenic 45.0%
CATGGTGCAGACATAGTTCA+AGG + chr4_4:73420876-73420895 Msa0701970:CDS 45.0%
AGTGTGTTCCAGTCTCATTG+CGG - chr4_4:73420932-73420951 None:intergenic 45.0%
CAATGAGACTGGAACACACT+TGG + chr4_4:73420932-73420951 Msa0701970:CDS 45.0%
TTGGATAATGTTGTGGCAGG+AGG - chr4_4:73421385-73421404 None:intergenic 45.0%
CCCACCCAGATTTATTCATC+TGG - chr4_4:73422070-73422089 None:intergenic 45.0%
ACCCAGTTAGTGGAGTAATG+CGG + chr4_4:73422206-73422225 Msa0701970:intron 45.0%
ACCGCATTACTCCACTAACT+GGG - chr4_4:73422210-73422229 None:intergenic 45.0%
ATTTGTGTGAGTGCATGAGC+AGG + chr4_4:73422514-73422533 Msa0701970:intron 45.0%
TGCATGAGCAGGCTATACAT+GGG + chr4_4:73422525-73422544 Msa0701970:intron 45.0%
CATGTGGAACGCAACCTAAT+AGG - chr4_4:73422586-73422605 None:intergenic 45.0%
TGTTGCAAAGTGGGGTCAAA+CGG + chr4_4:73423532-73423551 Msa0701970:CDS 45.0%
GCTTTGCACGGTTTCGTATT+AGG - chr4_4:73423568-73423587 None:intergenic 45.0%
TGCATTTCATCCAACTGAGG+TGG + chr4_4:73423612-73423631 Msa0701970:CDS 45.0%
!!! ATTATATTTATCTTAAATTT+TGG + chr4_4:73420979-73420998 Msa0701970:intron 5.0%
!!! TTTATTTTTAGTTTTTTTTT+TGG + chr4_4:73421799-73421818 Msa0701970:intron 5.0%
!!! TTATTTTTAGTTTTTTTTTT+GGG + chr4_4:73421800-73421819 Msa0701970:intron 5.0%
!!! TAAAAATTACTTTTTTTATA+TGG - chr4_4:73423168-73423187 None:intergenic 5.0%
!!! AAAAATATATATATAACTTT+TGG - chr4_4:73423378-73423397 None:intergenic 5.0%
! GATTTTCCTGCTGGTCCTAC+GGG + chr4_4:73420426-73420445 Msa0701970:CDS 50.0%
GTCAACTGAAGTACGGCCAT+TGG - chr4_4:73420460-73420479 None:intergenic 50.0%
AATTATGCATCCGGTGCAGC+AGG + chr4_4:73420903-73420922 Msa0701970:CDS 50.0%
ATTATGCATCCGGTGCAGCA+GGG + chr4_4:73420904-73420923 Msa0701970:CDS 50.0%
CAGGGTCCAGATATCAGCAT+GGG + chr4_4:73421127-73421146 Msa0701970:intron 50.0%
AGGGTCCAGATATCAGCATG+GGG + chr4_4:73421128-73421147 Msa0701970:intron 50.0%
TGCAACCCCATGCTGATATC+TGG - chr4_4:73421136-73421155 None:intergenic 50.0%
GACCGCATTACTCCACTAAC+TGG - chr4_4:73422211-73422230 None:intergenic 50.0%
GTGCATGAGCAGGCTATACA+TGG + chr4_4:73422524-73422543 Msa0701970:intron 50.0%
GGATATGCAAAGGATGGAGC+AGG - chr4_4:73423673-73423692 None:intergenic 50.0%
AATCTTCCCGTAGGACCAGC+AGG - chr4_4:73420435-73420454 None:intergenic 55.0%
CCATGCTGATATCTGGACCC+TGG - chr4_4:73421129-73421148 None:intergenic 55.0%
CCAGGGTCCAGATATCAGCA+TGG + chr4_4:73421126-73421145 Msa0701970:intron 55.0%
TAACTGGGGCGGAGTCCAAA+TGG - chr4_4:73421526-73421545 None:intergenic 55.0%
GAGCAGGCTATACATGGGCT+TGG + chr4_4:73422530-73422549 Msa0701970:intron 55.0%
ATTGCGGATCCCTGCTGCAC+CGG - chr4_4:73420916-73420935 None:intergenic 60.0%
GCAGGGATCCGCAATGAGAC+TGG + chr4_4:73420921-73420940 Msa0701970:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 73420209 73424035 73420209 ID=Msa0701970;Name=Msa0701970
chr4_4 mRNA 73420209 73424035 73420209 ID=Msa0701970-mRNA-1;Parent=Msa0701970;Name=Msa0701970-mRNA-1;_AED=0.29;_eAED=0.29;_QI=40|0.75|0.8|1|1|1|5|312|298
chr4_4 exon 73420209 73420486 73420209 ID=Msa0701970-mRNA-1:exon:22594;Parent=Msa0701970-mRNA-1
chr4_4 exon 73420829 73420953 73420829 ID=Msa0701970-mRNA-1:exon:22595;Parent=Msa0701970-mRNA-1
chr4_4 exon 73421130 73421213 73421130 ID=Msa0701970-mRNA-1:exon:22596;Parent=Msa0701970-mRNA-1
chr4_4 exon 73422536 73422782 73422536 ID=Msa0701970-mRNA-1:exon:22597;Parent=Msa0701970-mRNA-1
chr4_4 exon 73423521 73424035 73423521 ID=Msa0701970-mRNA-1:exon:22598;Parent=Msa0701970-mRNA-1
chr4_4 five_prime_UTR 73420209 73420248 73420209 ID=Msa0701970-mRNA-1:five_prime_utr;Parent=Msa0701970-mRNA-1
chr4_4 CDS 73420249 73420486 73420249 ID=Msa0701970-mRNA-1:cds;Parent=Msa0701970-mRNA-1
chr4_4 CDS 73420829 73420953 73420829 ID=Msa0701970-mRNA-1:cds;Parent=Msa0701970-mRNA-1
chr4_4 CDS 73421130 73421213 73421130 ID=Msa0701970-mRNA-1:cds;Parent=Msa0701970-mRNA-1
chr4_4 CDS 73422536 73422782 73422536 ID=Msa0701970-mRNA-1:cds;Parent=Msa0701970-mRNA-1
chr4_4 CDS 73423521 73423723 73423521 ID=Msa0701970-mRNA-1:cds;Parent=Msa0701970-mRNA-1
chr4_4 three_prime_UTR 73423724 73424035 73423724 ID=Msa0701970-mRNA-1:three_prime_utr;Parent=Msa0701970-mRNA-1
Gene Sequence

>Msa0701970

ATGGATCATAAGACTAAGTTATGGATGGTGCTTTTCTTCTTCTCAAGTGCAACATATTGTGTTGTTGGGAAGCCCCAAGTTCCATGTCTTTTCATTTTTGGAGATTCTTTATCGGACAGTGGAAACAACAATAACCTAGCTACCGATGCCAAAGTTAATTACAGGCCTTATGGGATTGATTTTCCTGCTGGTCCTACGGGAAGATTTACCAATGGCCGTACTTCAGTTGACATAATCACTGAACTTTTGGGATTTGACCATTTTATCCCACCTTATGCAAATACCCATGGTGCAGACATAGTTCAAGGTGTTAATTATGCATCCGGTGCAGCAGGGATCCGCAATGAGACTGGAACACACTTGGGTCCAGATATCAGCATGGGGTTGCAGTTACAACATCACACAGACATTGTTTCCAAAATTGCTAAGAAACTTGGATATGACAAAGCTATACATGGGCTTGGAGCAAGAAAGTTTTCTTTAGTTGGATTAGGCCTATTAGGTTGCGTTCCACATGAAATTTCTACCCATGGGAAAAATGATTCAAGGTGTATTCAAGAGGAGAATAATGCAGCACTACTTTTCAATGACAAACTTAAAGCCCTCGTTGATCGTTTGAATAAAGAATTAACTGATTCCAAATTCATCTTCATTAACAGTGCTGTCATAAGACATAGTCAGTTAAAGCTACCAGATCTTGTGAAATGTTGCAAAGTGGGGTCAAACGGACAGTGTATCCCTAATACGAAACCGTGCAAAGCAAGAAACCTACATCCATTTTTTGATGCATTTCATCCAACTGAGGTGGTCAACAAATTGTCTGCAAACTTAGCTTATAATGCTCCTGCTCCATCCTTTGCATATCCAATGGATATCTGTCGTCTTGTTAAATTGTAA
Protein sequence

>Msa0701970

MDHKTKLWMVLFFFSSATYCVVGKPQVPCLFIFGDSLSDSGNNNNLATDAKVNYRPYGIDFPAGPTGRFTNGRTSVDIITELLGFDHFIPPYANTHGADIVQGVNYASGAAGIRNETGTHLGPDISMGLQLQHHTDIVSKIAKKLGYDKAIHGLGARKFSLVGLGLLGCVPHEISTHGKNDSRCIQEENNAALLFNDKLKALVDRLNKELTDSKFIFINSAVIRHSQLKLPDLVKCCKVGSNGQCIPNTKPCKARNLHPFFDAFHPTEVVNKLSANLAYNAPAPSFAYPMDICRLVKL*