AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0621640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0621640 MtrunA17_Chr2g0286911 95.349 86 4 0 1 86 49 134 2.17e-55 167
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0621640 AT5G18920.1 72.727 55 15 0 22 76 22 76 1.25e-23 85.9
Msa0621640 AT5G18920.2 78.049 41 9 0 22 62 22 62 1.08e-17 70.9

Find 35 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCGGTTGCGTTGGCGCTAA+TGG 0.266671 4_2:+73818502 None:intergenic
GTTGGCCTCTTGACTTGATA+TGG 0.290122 4_2:-73817142 Msa0621640:three_prime_UTR
ATTGCATTGTTCGTAGTAAC+AGG 0.329052 4_2:-73818282 Msa0621640:CDS
GCTCCCTTTGACCCTTTGTA+AGG 0.337999 4_2:-73818597 Msa0621640:five_prime_UTR
TTGCCAAGCTCTCAGCTTGT+TGG 0.358345 4_2:-73817160 Msa0621640:three_prime_UTR
AACTGGAAAGAATGCCAAAA+AGG 0.380062 4_2:-73818259 Msa0621640:intron
AGAAGCCAAAGCCATTAAAA+TGG 0.395335 4_2:-73818557 Msa0621640:exon
AGGTCCTCTACTTTCTTCCT+AGG 0.400357 4_2:-73817434 Msa0621640:three_prime_UTR
TAAAATGTAAAAGATTAATT+CGG 0.402628 4_2:-73818672 None:intergenic
TTGTTCGTAGTAACAGGAAC+TGG 0.435270 4_2:-73818276 Msa0621640:CDS
AATTCGGTACATAAATTTCA+AGG 0.435466 4_2:-73818656 Msa0621640:five_prime_UTR
TTCTCTCTATCTCTTGATGC+AGG 0.438371 4_2:-73818419 Msa0621640:intron
TGGCAGAAATACAAGCTTTG+AGG 0.454509 4_2:-73817532 Msa0621640:intron
TTCTTCCTAGGCATTGTTCA+CGG 0.467556 4_2:-73817422 Msa0621640:three_prime_UTR
GGCAGAAATACAAGCTTTGA+GGG 0.476446 4_2:-73817531 Msa0621640:intron
AACCCTTACAAAGGGTCAAA+GGG 0.481232 4_2:+73818594 None:intergenic
CTAACCTCAAAACTAATCAT+TGG 0.482313 4_2:+73817271 None:intergenic
AGGCCAACAAGCTGAGAGCT+TGG 0.502300 4_2:+73817157 None:intergenic
TAACCCTTACAAAGGGTCAA+AGG 0.502429 4_2:+73818593 None:intergenic
CTCCCTTTGACCCTTTGTAA+GGG 0.502722 4_2:-73818596 Msa0621640:five_prime_UTR
TTCATGCTAATGGGTGGAAA+CGG 0.509298 4_2:+73817373 None:intergenic
TTGTGAAGTGGCGGTTGCGT+TGG 0.513985 4_2:+73818493 None:intergenic
TAATCATTGGTATTTCCTAT+CGG 0.537641 4_2:+73817284 None:intergenic
TGATCAATAACCCTTACAAA+GGG 0.557467 4_2:+73818586 None:intergenic
TTCGCTTCACTCGCGTTCAG+TGG 0.566822 4_2:+73818526 None:intergenic
ATGATCAATAACCCTTACAA+AGG 0.568443 4_2:+73818585 None:intergenic
TCTGCATCGCTCTTGTGAAG+TGG 0.571677 4_2:+73818481 None:intergenic
AAAATATTCATGCTAATGGG+TGG 0.585507 4_2:+73817367 None:intergenic
TTAAACCATATCAAGTCAAG+AGG 0.587613 4_2:+73817137 None:intergenic
TAAAGCAAAATCAAGACGAG+AGG 0.598086 4_2:+73817239 None:intergenic
AAATAAATAGTGCTTCCGAT+AGG 0.618950 4_2:-73817299 Msa0621640:three_prime_UTR
GCATCGCTCTTGTGAAGTGG+CGG 0.644641 4_2:+73818484 None:intergenic
TGCCACCGTGAACAATGCCT+AGG 0.662026 4_2:+73817417 None:intergenic
AATGCCTAGGAAGAAAGTAG+AGG 0.669007 4_2:+73817430 None:intergenic
TTCCTAGGCATTGTTCACGG+TGG 0.686904 4_2:-73817419 Msa0621640:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATATAGAAAACTAATAT+TGG + chr4_2:73818177-73818196 None:intergenic 10.0%
!! TTAATATAGAAAACTAATAT+TGG + chr4_2:73818177-73818196 None:intergenic 10.0%
!! TCAATGAATCAAAAAATTTA+GGG + chr4_2:73817426-73817445 None:intergenic 15.0%
!! ATCAATGAATCAAAAAATTT+AGG + chr4_2:73817427-73817446 None:intergenic 15.0%
!! TCAATGAATCAAAAAATTTA+GGG + chr4_2:73817426-73817445 None:intergenic 15.0%
!! ATCAATGAATCAAAAAATTT+AGG + chr4_2:73817427-73817446 None:intergenic 15.0%
!! CAATGAATCAAAAAATTTAG+GGG + chr4_2:73817425-73817444 None:intergenic 20.0%
!!! TTTTTTTCCTATTAATTCTC+AGG - chr4_2:73817460-73817479 Msa0621640:exon 20.0%
!!! ACAAAAGAATTATACCTTTT+TGG + chr4_2:73817523-73817542 None:intergenic 20.0%
!!! AACTTAATCGTTTAAGATAT+AGG + chr4_2:73817950-73817969 None:intergenic 20.0%
!! AGCAATTCATAAAATTACAA+TGG + chr4_2:73818206-73818225 None:intergenic 20.0%
!! AAACAGAAAGAAAAATAAAC+AGG - chr4_2:73818267-73818286 Msa0621640:CDS 20.0%
!! CAAAAAATATTCATGCTAAT+GGG + chr4_2:73818404-73818423 None:intergenic 20.0%
!! CAATGAATCAAAAAATTTAG+GGG + chr4_2:73817425-73817444 None:intergenic 20.0%
!!! TTTTTTTCCTATTAATTCTC+AGG - chr4_2:73817460-73817479 Msa0621640:exon 20.0%
!!! ACAAAAGAATTATACCTTTT+TGG + chr4_2:73817523-73817542 None:intergenic 20.0%
!!! AACTTAATCGTTTAAGATAT+AGG + chr4_2:73817950-73817969 None:intergenic 20.0%
!! AGCAATTCATAAAATTACAA+TGG + chr4_2:73818206-73818225 None:intergenic 20.0%
!! AAACAGAAAGAAAAATAAAC+AGG - chr4_2:73818267-73818286 Msa0621640:CDS 20.0%
!! CAAAAAATATTCATGCTAAT+GGG + chr4_2:73818404-73818423 None:intergenic 20.0%
! AATTCGGTACATAAATTTCA+AGG - chr4_2:73817109-73817128 Msa0621640:three_prime_UTR 25.0%
! TCAAAAAATTTAGGGGTTTA+GGG + chr4_2:73817418-73817437 None:intergenic 25.0%
! ATCAAAAAATTTAGGGGTTT+AGG + chr4_2:73817419-73817438 None:intergenic 25.0%
! GGAAAACTTATGAAAATAAG+CGG + chr4_2:73817859-73817878 None:intergenic 25.0%
!!! GCTATTTTCATAAGTAATCT+TGG + chr4_2:73817880-73817899 None:intergenic 25.0%
! AAACTTATGTTTAACCAAAC+AGG - chr4_2:73817923-73817942 Msa0621640:intron 25.0%
! AACTTATGTTTAACCAAACA+GGG - chr4_2:73817924-73817943 Msa0621640:intron 25.0%
!! TAGACTCTAGAAAATTGATT+TGG + chr4_2:73818073-73818092 None:intergenic 25.0%
!!! GAAGTTTTTAGTTAGTTATG+AGG - chr4_2:73818311-73818330 Msa0621640:intron 25.0%
! GCAAAAAATATTCATGCTAA+TGG + chr4_2:73818405-73818424 None:intergenic 25.0%
! TAATCATTGGTATTTCCTAT+CGG + chr4_2:73818484-73818503 None:intergenic 25.0%
!!! AATACCAATGATTAGTTTTG+AGG - chr4_2:73818490-73818509 Msa0621640:CDS 25.0%
! AATTCGGTACATAAATTTCA+AGG - chr4_2:73817109-73817128 Msa0621640:three_prime_UTR 25.0%
! TCAAAAAATTTAGGGGTTTA+GGG + chr4_2:73817418-73817437 None:intergenic 25.0%
! ATCAAAAAATTTAGGGGTTT+AGG + chr4_2:73817419-73817438 None:intergenic 25.0%
! GGAAAACTTATGAAAATAAG+CGG + chr4_2:73817859-73817878 None:intergenic 25.0%
!!! GCTATTTTCATAAGTAATCT+TGG + chr4_2:73817880-73817899 None:intergenic 25.0%
! AAACTTATGTTTAACCAAAC+AGG - chr4_2:73817923-73817942 Msa0621640:intron 25.0%
! AACTTATGTTTAACCAAACA+GGG - chr4_2:73817924-73817943 Msa0621640:intron 25.0%
!! TAGACTCTAGAAAATTGATT+TGG + chr4_2:73818073-73818092 None:intergenic 25.0%
!!! GAAGTTTTTAGTTAGTTATG+AGG - chr4_2:73818311-73818330 Msa0621640:intron 25.0%
! GCAAAAAATATTCATGCTAA+TGG + chr4_2:73818405-73818424 None:intergenic 25.0%
! TAATCATTGGTATTTCCTAT+CGG + chr4_2:73818484-73818503 None:intergenic 25.0%
!!! AATACCAATGATTAGTTTTG+AGG - chr4_2:73818490-73818509 Msa0621640:CDS 25.0%
TGATCAATAACCCTTACAAA+GGG + chr4_2:73817182-73817201 None:intergenic 30.0%
ATGATCAATAACCCTTACAA+AGG + chr4_2:73817183-73817202 None:intergenic 30.0%
ATGCAATCCTGAGAATTAAT+AGG + chr4_2:73817470-73817489 None:intergenic 30.0%
CTCACTTATAAACACTTCAT+TGG + chr4_2:73817661-73817680 None:intergenic 30.0%
TCGTAAGTTAATCCCAAAAA+TGG + chr4_2:73817689-73817708 None:intergenic 30.0%
TGCCTATACATAAAAGCTTA+TGG - chr4_2:73817772-73817791 Msa0621640:intron 30.0%
! GTCCATAAGCTTTTATGTAT+AGG + chr4_2:73817777-73817796 None:intergenic 30.0%
!! TTAAGTTTTGTGAGACTGTT+TGG - chr4_2:73817800-73817819 Msa0621640:intron 30.0%
AACCTGAGAATGTGTTTATA+AGG - chr4_2:73817999-73818018 Msa0621640:intron 30.0%
ACCTGAGAATGTGTTTATAA+GGG - chr4_2:73818000-73818019 Msa0621640:intron 30.0%
!!! TTTTCTAGAGTCTATTCATC+TGG - chr4_2:73818079-73818098 Msa0621640:intron 30.0%
AAAACTAATATTGGCAAGCA+TGG + chr4_2:73818168-73818187 None:intergenic 30.0%
TTTATGAATTGCTGATCTTG+TGG - chr4_2:73818213-73818232 Msa0621640:intron 30.0%
AAAATATTCATGCTAATGGG+TGG + chr4_2:73818401-73818420 None:intergenic 30.0%
AAATAAATAGTGCTTCCGAT+AGG - chr4_2:73818466-73818485 Msa0621640:CDS 30.0%
CTAACCTCAAAACTAATCAT+TGG + chr4_2:73818497-73818516 None:intergenic 30.0%
TTAAACCATATCAAGTCAAG+AGG + chr4_2:73818631-73818650 None:intergenic 30.0%
TGATCAATAACCCTTACAAA+GGG + chr4_2:73817182-73817201 None:intergenic 30.0%
ATGATCAATAACCCTTACAA+AGG + chr4_2:73817183-73817202 None:intergenic 30.0%
ATGCAATCCTGAGAATTAAT+AGG + chr4_2:73817470-73817489 None:intergenic 30.0%
CTCACTTATAAACACTTCAT+TGG + chr4_2:73817661-73817680 None:intergenic 30.0%
TCGTAAGTTAATCCCAAAAA+TGG + chr4_2:73817689-73817708 None:intergenic 30.0%
TGCCTATACATAAAAGCTTA+TGG - chr4_2:73817772-73817791 Msa0621640:intron 30.0%
! GTCCATAAGCTTTTATGTAT+AGG + chr4_2:73817777-73817796 None:intergenic 30.0%
!! TTAAGTTTTGTGAGACTGTT+TGG - chr4_2:73817800-73817819 Msa0621640:intron 30.0%
AACCTGAGAATGTGTTTATA+AGG - chr4_2:73817999-73818018 Msa0621640:intron 30.0%
ACCTGAGAATGTGTTTATAA+GGG - chr4_2:73818000-73818019 Msa0621640:intron 30.0%
!!! TTTTCTAGAGTCTATTCATC+TGG - chr4_2:73818079-73818098 Msa0621640:intron 30.0%
AAAACTAATATTGGCAAGCA+TGG + chr4_2:73818168-73818187 None:intergenic 30.0%
TTTATGAATTGCTGATCTTG+TGG - chr4_2:73818213-73818232 Msa0621640:intron 30.0%
AAAATATTCATGCTAATGGG+TGG + chr4_2:73818401-73818420 None:intergenic 30.0%
AAATAAATAGTGCTTCCGAT+AGG - chr4_2:73818466-73818485 Msa0621640:CDS 30.0%
CTAACCTCAAAACTAATCAT+TGG + chr4_2:73818497-73818516 None:intergenic 30.0%
TTAAACCATATCAAGTCAAG+AGG + chr4_2:73818631-73818650 None:intergenic 30.0%
AGAAGCCAAAGCCATTAAAA+TGG - chr4_2:73817208-73817227 Msa0621640:three_prime_UTR 35.0%
!! TTGCTCCATTTTAATGGCTT+TGG + chr4_2:73817216-73817235 None:intergenic 35.0%
! TGGTTGTTGCTCCATTTTAA+TGG + chr4_2:73817222-73817241 None:intergenic 35.0%
AGATGAAGAAGAAGAGAATG+AGG + chr4_2:73817387-73817406 None:intergenic 35.0%
! ATTTAGGGGTTTAGGGTTAT+GGG + chr4_2:73817411-73817430 None:intergenic 35.0%
! AATTTAGGGGTTTAGGGTTA+TGG + chr4_2:73817412-73817431 None:intergenic 35.0%
ATTGCATTGTTCGTAGTAAC+AGG - chr4_2:73817483-73817502 Msa0621640:CDS 35.0%
AACTGGAAAGAATGCCAAAA+AGG - chr4_2:73817506-73817525 Msa0621640:CDS 35.0%
!! TAAGTGAGAGAAGCCATTTT+TGG - chr4_2:73817673-73817692 Msa0621640:intron 35.0%
!!! AAGTGAGAGAAGCCATTTTT+GGG - chr4_2:73817674-73817693 Msa0621640:intron 35.0%
! TTTAAGATATAGGCCCTGTT+TGG + chr4_2:73817940-73817959 None:intergenic 35.0%
AGTTGTTTGTCCATACATGA+CGG - chr4_2:73817966-73817985 Msa0621640:intron 35.0%
TCCCTTATAAACACATTCTC+AGG + chr4_2:73818004-73818023 None:intergenic 35.0%
!! CGTCTAACAATCAGTTTTGT+TGG - chr4_2:73818035-73818054 Msa0621640:intron 35.0%
TAAAGCAAAATCAAGACGAG+AGG + chr4_2:73818529-73818548 None:intergenic 35.0%
AGAAGCCAAAGCCATTAAAA+TGG - chr4_2:73817208-73817227 Msa0621640:three_prime_UTR 35.0%
!! TTGCTCCATTTTAATGGCTT+TGG + chr4_2:73817216-73817235 None:intergenic 35.0%
! TGGTTGTTGCTCCATTTTAA+TGG + chr4_2:73817222-73817241 None:intergenic 35.0%
AGATGAAGAAGAAGAGAATG+AGG + chr4_2:73817387-73817406 None:intergenic 35.0%
! ATTTAGGGGTTTAGGGTTAT+GGG + chr4_2:73817411-73817430 None:intergenic 35.0%
! AATTTAGGGGTTTAGGGTTA+TGG + chr4_2:73817412-73817431 None:intergenic 35.0%
ATTGCATTGTTCGTAGTAAC+AGG - chr4_2:73817483-73817502 Msa0621640:CDS 35.0%
AACTGGAAAGAATGCCAAAA+AGG - chr4_2:73817506-73817525 Msa0621640:CDS 35.0%
!! TAAGTGAGAGAAGCCATTTT+TGG - chr4_2:73817673-73817692 Msa0621640:intron 35.0%
!!! AAGTGAGAGAAGCCATTTTT+GGG - chr4_2:73817674-73817693 Msa0621640:intron 35.0%
! TTTAAGATATAGGCCCTGTT+TGG + chr4_2:73817940-73817959 None:intergenic 35.0%
AGTTGTTTGTCCATACATGA+CGG - chr4_2:73817966-73817985 Msa0621640:intron 35.0%
TCCCTTATAAACACATTCTC+AGG + chr4_2:73818004-73818023 None:intergenic 35.0%
!! CGTCTAACAATCAGTTTTGT+TGG - chr4_2:73818035-73818054 Msa0621640:intron 35.0%
TAAAGCAAAATCAAGACGAG+AGG + chr4_2:73818529-73818548 None:intergenic 35.0%
AACCCTTACAAAGGGTCAAA+GGG + chr4_2:73817174-73817193 None:intergenic 40.0%
TAACCCTTACAAAGGGTCAA+AGG + chr4_2:73817175-73817194 None:intergenic 40.0%
TTCTCTCTATCTCTTGATGC+AGG - chr4_2:73817346-73817365 Msa0621640:three_prime_UTR 40.0%
TATCTCTTGATGCAGGTAGT+AGG - chr4_2:73817353-73817372 Msa0621640:three_prime_UTR 40.0%
GAAGAAGAAGAGAATGAGGT+TGG + chr4_2:73817383-73817402 None:intergenic 40.0%
TTGTTCGTAGTAACAGGAAC+TGG - chr4_2:73817489-73817508 Msa0621640:CDS 40.0%
ACACATAGAGAGAGAGAAAG+AGG + chr4_2:73817552-73817571 None:intergenic 40.0%
TATGTGTGAGACTGTGATGT+AGG - chr4_2:73817565-73817584 Msa0621640:intron 40.0%
ATGTGTGAGACTGTGATGTA+GGG - chr4_2:73817566-73817585 Msa0621640:intron 40.0%
AGACTGTTTGGAAGAACTTG+TGG - chr4_2:73817812-73817831 Msa0621640:intron 40.0%
GTTGTTTGTCCATACATGAC+GGG - chr4_2:73817967-73817986 Msa0621640:intron 40.0%
TTGTTTGTCCATACATGACG+GGG - chr4_2:73817968-73817987 Msa0621640:intron 40.0%
TCATATATCCCCGTCATGTA+TGG + chr4_2:73817979-73817998 None:intergenic 40.0%
!!! CAGTTTTGTTGGATTGAGTG+CGG - chr4_2:73818046-73818065 Msa0621640:intron 40.0%
TGGCAGAAATACAAGCTTTG+AGG - chr4_2:73818233-73818252 Msa0621640:intron 40.0%
! GGCAGAAATACAAGCTTTGA+GGG - chr4_2:73818234-73818253 Msa0621640:intron 40.0%
AATGCCTAGGAAGAAAGTAG+AGG + chr4_2:73818338-73818357 None:intergenic 40.0%
! TTCTTCCTAGGCATTGTTCA+CGG - chr4_2:73818343-73818362 Msa0621640:intron 40.0%
! TTCATGCTAATGGGTGGAAA+CGG + chr4_2:73818395-73818414 None:intergenic 40.0%
AACCCTTACAAAGGGTCAAA+GGG + chr4_2:73817174-73817193 None:intergenic 40.0%
TAACCCTTACAAAGGGTCAA+AGG + chr4_2:73817175-73817194 None:intergenic 40.0%
TTCTCTCTATCTCTTGATGC+AGG - chr4_2:73817346-73817365 Msa0621640:three_prime_UTR 40.0%
TATCTCTTGATGCAGGTAGT+AGG - chr4_2:73817353-73817372 Msa0621640:three_prime_UTR 40.0%
GAAGAAGAAGAGAATGAGGT+TGG + chr4_2:73817383-73817402 None:intergenic 40.0%
TTGTTCGTAGTAACAGGAAC+TGG - chr4_2:73817489-73817508 Msa0621640:CDS 40.0%
ACACATAGAGAGAGAGAAAG+AGG + chr4_2:73817552-73817571 None:intergenic 40.0%
TATGTGTGAGACTGTGATGT+AGG - chr4_2:73817565-73817584 Msa0621640:intron 40.0%
ATGTGTGAGACTGTGATGTA+GGG - chr4_2:73817566-73817585 Msa0621640:intron 40.0%
AGACTGTTTGGAAGAACTTG+TGG - chr4_2:73817812-73817831 Msa0621640:intron 40.0%
GTTGTTTGTCCATACATGAC+GGG - chr4_2:73817967-73817986 Msa0621640:intron 40.0%
TTGTTTGTCCATACATGACG+GGG - chr4_2:73817968-73817987 Msa0621640:intron 40.0%
TCATATATCCCCGTCATGTA+TGG + chr4_2:73817979-73817998 None:intergenic 40.0%
!!! CAGTTTTGTTGGATTGAGTG+CGG - chr4_2:73818046-73818065 Msa0621640:intron 40.0%
TGGCAGAAATACAAGCTTTG+AGG - chr4_2:73818233-73818252 Msa0621640:intron 40.0%
! GGCAGAAATACAAGCTTTGA+GGG - chr4_2:73818234-73818253 Msa0621640:intron 40.0%
AATGCCTAGGAAGAAAGTAG+AGG + chr4_2:73818338-73818357 None:intergenic 40.0%
! TTCTTCCTAGGCATTGTTCA+CGG - chr4_2:73818343-73818362 Msa0621640:intron 40.0%
! TTCATGCTAATGGGTGGAAA+CGG + chr4_2:73818395-73818414 None:intergenic 40.0%
CTCCCTTTGACCCTTTGTAA+GGG - chr4_2:73817169-73817188 Msa0621640:three_prime_UTR 45.0%
AGGTCCTCTACTTTCTTCCT+AGG - chr4_2:73818331-73818350 Msa0621640:intron 45.0%
! GTTGGCCTCTTGACTTGATA+TGG - chr4_2:73818623-73818642 Msa0621640:five_prime_UTR 45.0%
CTCCCTTTGACCCTTTGTAA+GGG - chr4_2:73817169-73817188 Msa0621640:three_prime_UTR 45.0%
AGGTCCTCTACTTTCTTCCT+AGG - chr4_2:73818331-73818350 Msa0621640:intron 45.0%
! GTTGGCCTCTTGACTTGATA+TGG - chr4_2:73818623-73818642 Msa0621640:five_prime_UTR 45.0%
GCTCCCTTTGACCCTTTGTA+AGG - chr4_2:73817168-73817187 Msa0621640:three_prime_UTR 50.0%
TCTGCATCGCTCTTGTGAAG+TGG + chr4_2:73817287-73817306 None:intergenic 50.0%
! TTCCTAGGCATTGTTCACGG+TGG - chr4_2:73818346-73818365 Msa0621640:intron 50.0%
TTGCCAAGCTCTCAGCTTGT+TGG - chr4_2:73818605-73818624 Msa0621640:five_prime_UTR 50.0%
GCTCCCTTTGACCCTTTGTA+AGG - chr4_2:73817168-73817187 Msa0621640:three_prime_UTR 50.0%
TCTGCATCGCTCTTGTGAAG+TGG + chr4_2:73817287-73817306 None:intergenic 50.0%
! TTCCTAGGCATTGTTCACGG+TGG - chr4_2:73818346-73818365 Msa0621640:intron 50.0%
TTGCCAAGCTCTCAGCTTGT+TGG - chr4_2:73818605-73818624 Msa0621640:five_prime_UTR 50.0%
TTCGCTTCACTCGCGTTCAG+TGG + chr4_2:73817242-73817261 None:intergenic 55.0%
TTGTGAAGTGGCGGTTGCGT+TGG + chr4_2:73817275-73817294 None:intergenic 55.0%
GCATCGCTCTTGTGAAGTGG+CGG + chr4_2:73817284-73817303 None:intergenic 55.0%
TGCCACCGTGAACAATGCCT+AGG + chr4_2:73818351-73818370 None:intergenic 55.0%
AGGCCAACAAGCTGAGAGCT+TGG + chr4_2:73818611-73818630 None:intergenic 55.0%
TTCGCTTCACTCGCGTTCAG+TGG + chr4_2:73817242-73817261 None:intergenic 55.0%
TTGTGAAGTGGCGGTTGCGT+TGG + chr4_2:73817275-73817294 None:intergenic 55.0%
GCATCGCTCTTGTGAAGTGG+CGG + chr4_2:73817284-73817303 None:intergenic 55.0%
TGCCACCGTGAACAATGCCT+AGG + chr4_2:73818351-73818370 None:intergenic 55.0%
AGGCCAACAAGCTGAGAGCT+TGG + chr4_2:73818611-73818630 None:intergenic 55.0%
! GGCGGTTGCGTTGGCGCTAA+TGG + chr4_2:73817266-73817285 None:intergenic 65.0%
! GGCGGTTGCGTTGGCGCTAA+TGG + chr4_2:73817266-73817285 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 73817106 73818681 73817106 ID=Msa0621640;Name=Msa0621640
chr4_2 mRNA 73817106 73818681 73817106 ID=Msa0621640-mRNA-1;Parent=Msa0621640;Name=Msa0621640-mRNA-1;_AED=0.44;_eAED=0.44;_QI=121|1|1|1|1|1|3|369|86
chr4_2 exon 73817106 73817548 73817106 ID=Msa0621640-mRNA-1:exon:22193;Parent=Msa0621640-mRNA-1
chr4_2 exon 73818260 73818305 73818260 ID=Msa0621640-mRNA-1:exon:22192;Parent=Msa0621640-mRNA-1
chr4_2 exon 73818420 73818681 73818420 ID=Msa0621640-mRNA-1:exon:22191;Parent=Msa0621640-mRNA-1
chr4_2 five_prime_UTR 73818561 73818681 73818561 ID=Msa0621640-mRNA-1:five_prime_utr;Parent=Msa0621640-mRNA-1
chr4_2 CDS 73818420 73818560 73818420 ID=Msa0621640-mRNA-1:cds;Parent=Msa0621640-mRNA-1
chr4_2 CDS 73818260 73818305 73818260 ID=Msa0621640-mRNA-1:cds;Parent=Msa0621640-mRNA-1
chr4_2 CDS 73817475 73817548 73817475 ID=Msa0621640-mRNA-1:cds;Parent=Msa0621640-mRNA-1
chr4_2 three_prime_UTR 73817106 73817474 73817106 ID=Msa0621640-mRNA-1:three_prime_utr;Parent=Msa0621640-mRNA-1
Gene Sequence

>Msa0621640

ATGGAGCAACAACCACTGAACGCGAGTGAAGCGAAACCATTAGCGCCAACGCAACCGCCACTTCACAAGAGCGATGCAGATGACGATGATGAGAACGTCAAGCAGCTCGATGAGTGTTCTTCTCTCTATCTCTTGATGCAGGATTGCATTGTTCGTAGTAACAGGAACTGGAAAGAATGCCAAAAAGAAATACAAGCTTTGAGGGAATGCTCTGAAAACAGAAAGAAAAATAAACAGGAAGTCAGAGTTCATAAGCAATGA
Protein sequence

>Msa0621640

MEQQPLNASEAKPLAPTQPPLHKSDADDDDENVKQLDECSSLYLLMQDCIVRSNRNWKECQKEIQALRECSENRKKNKQEVRVHKQ*